Gene expression profiling of epidermal cell types in C. elegans using Targeted DamID.

The epidermis of Caenorhabditis elegans is an essential tissue for survival because it contributes to the formation of the cuticle barrier as well as facilitating developmental progression and animal growth. Most of the epidermis consists of the hyp7 hypodermal syncytium, the nuclei of which are largely generated by the seam cells, which exhibit stem cell-like behaviour during development. How seam cell progenitors differ transcriptionally from the differentiated hypodermis is poorly understood. Here, we introduce Targeted DamID (TaDa) in C. elegans as a method for identifying genes expressed within a tissue of interest without cell isolation. We show that TaDa signal enrichment profiles can be used to identify genes transcribed in the epidermis and use this method to resolve differences in gene expression between the seam cells and the hypodermis. Finally, we predict and functionally validate new transcription and chromatin factors acting in seam cell development. These findings provide insights into cell type-specific gene expression profiles likely associated with epidermal cell fate patterning.

The impact of cognitive flexibility on prospective EFL teachers' critical thinking disposition: the mediating role of self-efficacy.

Critical thinking as one of the key skills for success in the 21st-century has been considered by many scholars in teacher education. This study tries to examine the interaction of critical thinking disposition with two other key characteristics of successful teachers: cognitive flexibility and self-efficacy. To this end, a sample of pre-service English as a Foreign Language (EFL) teachers was selected for this study. Based on the findings, a positive and strong relationship between cognitive flexibility and critical thinking disposition, and a positive and robust correlation between self-efficacy and critical thinking disposition were observed. Hence, it can be suggested that teacher-educationists can use this link for designing teacher-training courses with tailored tasks for both in and pre-service teachers. The main contribution of the findings might be beneficial for homogenizing teacher-training courses around the globe with the 21st-century trends. In addition, this line of research can be followed by empirical studies for checking the effectiveness of tailored tasks for provoking teachers' critical thinking dispositions, cognitive flexibility, and self-efficacy in teaching activities.

The development of English language connected speech perception skills: an empirical study on Chinese EFL children.

Connected speech processes (CSPs) occur randomly in everyday conversations of native speakers; however, such phonological variations can bring about challenges for non-native listeners. Looking at CSP literature, there seems to be very few studies that involved young foreign language learners. Therefore, the present study aimed to explore the development of connected speech perception skills by focusing on 201 9- to 12-year-old Chinese EFL children. It also incorporated systematic error analysis to further probe into the specific perceptual difficulties. The results indicate that: (1) Despite a significantly ascending trend for the overall growth of perception skills, no significant differences were found between 11 and 12 year olds in elision and contraction, which suggests that the developmental trend varied depending on different CSP types; (2) Although random errors decreased with age, the number of lexicon and syntax errors gradually increased, and the distribution of perceptual errors shifted from the level of words and syllables to that of phonemes; (3) The primary types of errors resulting in the perception difficulties for elision and contraction were consonant errors, grammatical errors and morphology errors. Ergo, this study enhances the understanding of connected speech perception among EFL children and provides some implications for EFL/ESL listening instructions.

The Effect of Metacognitive Intervention on the Listening Performance and Metacognitive Awareness of High- and Low-Working Memory Capacity EFL Learners.

EFL listening comprehension has been a stark challenge for language learners, but little is known about the combined effect of individual differences, such as working memory capacity, and metacognitive intervention. Thus, the present experimental study investigates the effect of metacognitive intervention on the listening performance and metacognitive awareness of high- and low-WMC EFL learners. For this purpose, Oxford Placement Tests were distributed among 120 male Iranian EFL learners, of which 94 were identified as intermediate. Then, backward visual digit span tests were administered to measure their working memory capacity. Based on the median of all scores, 80 learners were selected and randomly assigned to two experimental groups and two control groups each with 20 participants. Next, their performance on the International English Language Testing System and the Metacognitive Awareness Listening Questionnaire was measured before and after the 8-session metacognitive intervention. Results showed that high-WMC experimental learners had a higher gain with a large effect size in terms of listening performance compared with all the other groups. In addition, the experimental group learners reported the significantly higher use of the metacognitive strategies with a moderate effect size. Interestingly, low-WMC learners' listening performance and metacognitive awareness also improved as a result of the intervention. Our findings bear pedagogical significance in that individual differences in WMC should be considered more in both EFL language classes and the future line of research involving the metacognitive intervention.

Unpacking Chinese EFL Students' Academic Engagement and Psychological Well-Being: The Roles of Language Teachers' Affective Scaffolding.

Over the past decade, there has appeared a surge of research interest in language learners' academic engagement and psychological well-being as important factors in improving the quality of education. However, research on the roles of English as a foreign language (EFL) teachers' affective scaffolding in enhancing the academic engagement and psychological well-being of their students is relatively scant. Inspired by this gap, the current study aimed to investigate the impact of Chinese EFL teachers' affective scaffolding on their learners' academic engagement and psychological well-being. To this end, a total number of 1968 Chinese EFL learners participated in this questionnaire survey. The results of the study showed that EFL teachers' affective scaffolding positively and significantly predicted students' academic engagement and psychological well-being. More specifically, it was found that teachers' affective scaffolding explained about 73% and 65% of variances in EFL students' academic engagement and psychological well-being. Moreover, it was found that psychological well-being and academic engagement were positively correlated and predicted 56% of each other's variances. In accordance with these findings, educators are recommended to build up a harmonious teacher-student relationship to foster students' psych-emotional development.

Exploring Academically Transitioning Students' Ongoing Experiences with Textbook-Based Learning: A Semester-Long Study.

This study reports on the ongoing experiences of academically transitioning students with textbook-based learning. The study was conducted at a Chinese university among first-semester English-as-a-foreign-language (EFL) students transitioning from high school to university. Qualitative analysis of interviews with the students, students' written reflections, and field notes about their learning activities revealed that their paths were not linear; instead, they experienced both progression and regression in textbook-based learning during their academic transition. Their initial passion for learning in a new context was soon replaced by their need to adjust, primarily due to differences between their prior and current learning experiences and the language demands. The students' adjustments were supported by their own agency and the use of adapted instructions. The study concludes that the students' ongoing experiences with textbook-based learning were complex and dynamic, but the students were also amenable to adjustment.

Teachers' Content, Pedagogical, and Technological Knowledge, and the Use of Technology in Teaching Pronunciation.

This study examined the correlations between three vital areas of teaching pronunciation in English as a Foreign Language (EFL) classes (i.e., content, pedagogical, and technological knowledge). This study also explored the relationships between teachers' majors, experience, and technology competence with using technology in teaching English pronunciation. Data was collected by using a questionnaire. The study tool was a model adapted from different studies. The participants of the study were sixty English language instructors at different Saudi universities. The result indicated that there was a statistically significant difference in the three constructs of the model based on the participants' technology competence. The results revealed that content knowledge had a small correlation with pedagogical knowledge, and with technological knowledge. Pedagogical knowledge had a strong positive correlation with technological knowledge.

A Comparative Study on the Impact of Lexical Inferencing, Extended Audio Glossing, and Frequency Mode of Input Instruction on EFL Learners' Lexical Collocation Knowledge.

This study shed light on the effects of four modes of vocabulary instruction, i.e., extended audio glossing, lexical inferencing, lexical translation, and frequency manipulation of input on the learning of lexical collocations by Iranian intermediate EFL learners. In so doing, 80 L1 Persian EFL students were divided into four 20-participant comparison groups: Lexical Inferencing (LI), Extended Audio Glossing (EAG), Frequency Manipulation of Input (FM), and the Lexical Translation group (LT). LI, EAG, FM, and LT were treated through lexical inferencing, extended audio glossing, skewed frequency of input, and lexical translation techniques, respectively. The participants were pretested and posttested through a piloted multiple-choice lexical collocation test and instructed for ten instructional sessions. The results of the data, analyzed through repeated measures ANCOVA, showed that all the techniques examined in this study were effective on learners' achievement in lexical collocations. Comparatively, FM treated through frequency manipulation of input, significantly outperformed the other groups in lexical collocation improvement. The ANCOVA results and paired comparisons also indicated that EAG had the least achievement in lexical collocation compared to the other three groups. These results can hopefully inform language teachers, learners, and syllabus designers.

EFL Listening, Metacognitive Awareness, and Motivation: The Magic of L1-Mediated Metacognitive Intervention.

This study strove to investigate the effect of L1-mediated metacognitive intervention (MI) on the listening comprehension performance, metacognitive awareness, and motivation of Iranian EFL learners. The participants were 330 upper-intermediate EFL learners in three groups, ranging from 17 to 25 years of age. The experimental groups (Ex1 = 110/Ex2 = 110) went through a guided lesson plan in metacognition in L2 (English) and L1 (Persian) for seventeen weeks, which focused on planning, monitoring, and evaluation. The control group (CG = 110), also instructed by the same teacher, listened to the same texts in L2 (English) without any guided attention to process. An actual IELTS TEST, the Metacognitive Awareness Listening Questionnaire (MALQ) and a motivation questionnaire (LLOS-IEA) were used before and after the intervention to track changes in the listening performance, metacognitive awareness, and motivation. The overall results showed that MI caused a considerable variance in the listening performance, metacognitive awareness, and motivation in both experimental groups. The results also illustrated that the medium for the delivery of metacognitive intervention (L1) assisted the learners in experimental group two, who went through L1-mediated metacognitive intervention, to outperform their peers in experimental group one, who were taught in L2, and the control group, who were taught conventionally.

The Case for Smiling? Nonverbal Behavior and Oral Corrective Feedback.

Oral Corrective Feedback is a widely used teaching strategy that has been found to help language acquisition. The factors that contribute to its effectiveness, however, remain elusive. In this study, the role of smiling during teachers' OCF provision is investigated in intact language classrooms by modifying the analytical framework developed by Lyster and Ranta (Stud Second Lang Acquis, 19(1):37-66, 1997), which determines OCF effectiveness by the success of the learner uptake. In addition to the feedback strategies used, this study examines teacher smiling during the feedback instances, and whether they were genuine or polite smiles. The Facial Action Coding System (Ekman & Friesen, Environ Psych Nonver, 1(1), 56-75, 1976; Ekman, Friesen, & Hager, Facial Action Coding System: The Manual on CD ROM. Salt Lake City, UT: Research Nexus division of Network Information Research Corporation, 2002) is utilized to operationalize smile genuineness. Significant findings indicate that when teacher smiling is genuine, learners are more likely to correct their errors, while polite smiles do not have the same effect.

Effects of Group-Dynamic Assessment and Process-Based Instruction on EFL Learners' Metacognitive Awareness and Listening Comprehension: A Mixed-Methods Inquiry.

Although a range of studies has explored the effectiveness of group-dynamic assessment (G-DA) and process-based instruction (PBI) in second language (L2) learning, no study has compared the effects of G-DA and PBI on EFL learners' metacognitive awareness (MA) and listening comprehension (LC). Thus, this study aimed to explore the effects of G-DA and PBI on fostering EFL learners' metacognitive awareness (MA) and listening comprehension (LC) in Iran. For this purpose, a total of one hundred and sixty intermediate EFL learners were selected through a convenience sampling method at Iran Language Institute (ILI) and were homogenized using the Key English Test (KET). The EFL learners whose scores fell around the mean score were chosen and randomly allocated as G-DA group (n = 30), PBI group (n = 30), and control group (n = 30). Afterward, they went through a pre-test, interventions (lasting 16 one-hour sessions held twice a week) and a post-test. The interactions in the classes were also meticulously recorded. The collected data were analyzed using a one-way ANOVA and a microgenetic development approach. Findings evidenced that the G-DA group and PBI group outperformed the control group concerning the gains in MA and LC. However, the findings evidenced that G-DA was more effective than PBI to foster the EFL learners' MA and LC. Additionally, the complementary qualitative results documented that the proper feedback offered in line with the principles and procedures of G-DA and PBI contributed to developing the participants' MA and LC. The study ends by offering some implications for the relevant stakeholders.

The Structural Relationship Between Teacher Support and Willingness to Communicate: The Mediation of L2 Anxiety and the Moderation of Growth Language Mindset.

The important role of willingness to communicate (WTC) in facilitating second language (L2) learning and use has been widely endorsed. However, few studies have examined how teacher support in an L2 class may predict students' L2 WTC. Such a relationship may also be mediated by learners' L2 anxiety, a typical predictor of L2 WTC, and moderated by learners' beliefs about the malleability of their language learning ability, a construct known as growth language mindset. Framed from the Control-Value Theory (Pekrun, in Educ Psychol Rev 18(4):315-341, 2006) and the Language-Mindset Meaning System (Lou and Noels, in: Lamb, Csizér, Henry, Ryan (eds) The Palgrave handbook of motivation for language learning, Palgrave Macmillan, 2019a, System 86:102126, 2019b), this study aimed to investigate the relationships between teacher support, L2 anxiety, growth language mindset, and L2 WTC. The data were collected from 551 English-as-a-Foreign-Language (EFL) learners in Iran and analyzed using structural equation modeling (SEM). The results showed that teacher support was directly and positively associated with L2 WTC, and this relationship was significantly mediated by L2 anxiety. The relationship between teacher support and L2 WTC, however, was only significant among learners with medium and high levels of growth language mindset. In addition, growth language mindset also moderated the negative relationship between L2 anxiety and L2 WTC, with this relationship being weaker among learners with higher levels of growth language mindset. Finally, theoretical and pedagogical implications and directions for future research are presented.

"Should I change myself or not?": Examining (Re)constructed language teacher identity during the COVID-19 pandemic through text-mining.

On the basis of interviews with 20 Chinese EFL teachers and text-mining analysis, this study investigates the reconstruction of language teacher identity during the COVID-19 pandemic in terms of context, agency, and practice. The word co-occurrence analysis and collocation statistics with modal verbs coded by KH Coder 3.0 demonstrate that English teachers face two types of identity reconstruction processes: (a) situational context related to various changes, and, (b) interactional context that causes communication difficulties with students. How teachers enact agency is influenced by their teaching experience. Through practice, teachers reconstruct their identity as solution seekers, positive learners, and confident professionals.

Probing into the boredom of online instruction among Chinese English language teachers during the Covid-19 pandemic.

Although researching emotions in language education has dramatically increased during the past decades, little is written about the effects of aversive feelings like teacher boredom in the English as a foreign language (EFL) context, especially in the context of online instruction during the COVID-19 pandemic. Against this shortcoming, this study examined the causes of and solutions to teacher boredom experienced by 216 Chinese EFL teachers, including both genders with their ages ranging from 19 to 58. In so doing, the researcher used maximum variation sampling to gather the data via an open-ended questionnaire and semi-structured interviews. The gleaned data were then thematically analyzed by MAXQDA (Version 2020) whose results indicated that most participants consider the online mode of delivery more boring than the face-to-face mode. Additionally, the findings demonstrated that both the antecedents of and the solutions to teacher boredom come under the macro-categories of student-related, task-related, IT-related, and teacher-related factors and solutions. Out of these, IT-related factors and teacher-related solutions were the most frequently raised themes extracted from the data. The study presents some practical implications and directions for future research.

Modeling the contributions of EFL university professors' emotion regulation to self-efficacy, work engagement, and anger.

As university professors' emotion regulation (ER) plays a pivotal role in their efficacy, it is essential to explore if it is significantly correlated with work engagement (WE), self-efficacy, and anger at the workplace. To respond to the call, this research inspected the contributions of university professors' ER to their WE, self-efficacy, and anger. For this purpose, a total of 278 university professors were selected using a convenience sampling method and asked to fill out Language Teacher Emotion Regulation Inventory, Engaged Teacher Scale, Teacher Sense of Efficacy Scale, and Teacher Anger Scale. A structural equation modeling was used to analyze the gathered data. Results disclosed that the participants' ER significantly contributed to their WE and self-efficacy. Additionally, the findings documented that the participants' ER was negatively correlated with their anger. The implications of the findings are discussed for pertinent stakeholders.

Exploring the effects of online learning on EFL learners' motivation, anxiety, and attitudes during the COVID-19 pandemic: a focus on Iran.

With the emergence and dissemination of the COVID-19 pandemic and the transformation of education to online classes, it is important to explore the effects of online learning on EFL learners' psychological factors in the Iranian EFL learners. Hence, this study aims to explore the effects of online learning on EFL learners' motivation, anxiety, and attitude. For this purpose, using a convenience sampling method, a total of 293 upper-intermediate EFL learners at Iran Language Institute in Ahvaz, Iran, took the Oxford Quick Placement Test, and 200 EFL learners whose scores fell around the mean were selected and assigned randomly to an experimental group and a control group. The participants' motivation, anxiety, and attitudes were measured before and after the treatments (lasting 10 one-hour sessions held once a week) using validated questionnaires. The collected data were analyzed through a one-way MANOVA test and a one-sample t-test. The findings evidenced that online learning positively affected the participants' motivation, anxiety, and attitudes. That is, due to the online learning, their motivation increased, their anxiety lowered, and positive attitudes were shaped toward L2 learning. Finally, based on the findings, some implications are proposed.

Social support in class and learning burnout among Chinese EFL learners in higher education: Are academic buoyancy and class level important?

The prevalence and adverse effects of learning burnout are a major concern in higher education. Based upon JD-R and COR theories, this study modeled the associations among social support that teachers and peers provide in class, academic buoyancy, learning burnout, and class level with respect to the degree of English proficiency. A sample of 1955 Chinese EFL learners in higher education participated in the cross-sectional survey. Structural equation modelling via partial least squares technique was utilized for statistical analysis. The results corroborated the protecting role that social support in class played against EFL students' learning burnout. In particular, the findings revealed that academic buoyancy both mediated and moderated the nexus between social support on EFL learners' burnout. Moreover, this study found that class level with respect to English proficiency moderated the relation between academic buoyancy and learning burnout and that the negative impact of academic buoyancy on burnout increased in classes in which students had lower English proficiency. Based upon the findings, certain targeted suggestions for educational practice were provided.

Influencing factors of Chinese EFL students' continuance learning intention in SPOC-based blended learning environment.

The online and offline blended learning mode based on SPOC has gradually become a popular teaching mode in higher institutions due to COVID-19 pandemic. However, students of English as a Foreign Language (EFL) in SPOC-based blended learning environments continue facing problems of low participation and persistent intention. In order to explore the influencing factors of EFL students' continuance intentions to learn under the SPOC blended learning environment, this study recruits 48 EFL students from three higher vocational colleges based on the grounded theory. Through the triple coding method of open, axis, and selective coding based on the grounded theory and the used of software Nvivo to conduct a triple coding analysis of the text data obtained from the in-depth interviews and focus group interviews, a theoretical model of the influencing factors of EFL students' continuous learning intention (pre-influencing factors, external situational factors, and continuance intention) is proposed. Besides, a systematic framework based on stakeholders response to improve EFL students' continuance learning intentions in SPOC-based blended learning environments is constructed accordingly. This study can provide reference for basic theory and variable selection for subsequent research on the influencing factors of EFL students' continuance learning intentions in China and elsewhere.

A triangulation method on the effectiveness of digital game-based language learning for vocabulary acquisition.

This research aimed to explore the views of 8th grade students on digital game-based English language learning as a foreign language (EFL). A total of 69 students between the ages of 12 and 14 participated in the study. Students' vocabulary acquisition skills were tested using a web 2.0 application called "Quizziz." The study utilized a triangulation method in which the results of a quasi-experiment and the metaphorical perceptions of the learners were obtained. The results of the tests were recorded at two-week intervals, and students' reactions to the results were collected using a data collection tool. The study used a pre-test, post-test, and control group design. The experimental and control groups took a pre-test before the study began. The experimental group then practiced vocabulary using Quizziz, while the control group practiced vocabulary by memorizing it in their mother tongue. The post-test results showed significant differences between the control and experimental groups. In addition, content analysis was used to analyse the data, with metaphors being grouped and frequencies calculated. The students generally expressed positive views on digital game-based EFL, stating that it was highly successful and that in-game power-ups, competing with other students, and receiving rapid feedback improved their motivation.

University-level EFL students' views on learning English online: a qualitative study.

The main aim of this article is to determine Turkish EFL (English as a foreign language) students' perceptions about learning English through distance education (henceforth DE) during the COVID-19 pandemic. To realize this aim, the study adopted a qualitative research design and used the metaphor elicitation technique, semi-structured telephone interviews, and an online focus group interview to collect data. The results of the analysis show that most of the students prefer face-to-face English education in a classroom setting rather than online education. Despite some positive comments about DE like it being more comfortable for shy students, students' attitudes toward DE were found to be mostly negative because of the challenges they faced like technical problems, health and focus issues, a lack of interaction and speaking practice in the target language, feelings of anxiety and isolation and low motivation, and DE not addressing all learning styles effectively. Using several qualitative data collection tools, this study provides valuable insights into the thoughts and experiences of students about online English language learning, which can be taken into account to enhance future online language teaching and learning efforts. This study also offers some suggestions to make online EFL education more productive and effective.