RESUMO
Between 2011 and 2013, three breeders of variegated squirrels (Sciurus variegatoides) had encephalitis with similar clinical signs and died 2 to 4 months after onset of the clinical symptoms. With the use of a metagenomic approach that incorporated next-generation sequencing and real-time reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR), the presence of a previously unknown bornavirus was detected in a contact squirrel and in brain samples from the three patients. Phylogenetic analyses showed that this virus, tentatively named variegated squirrel 1 bornavirus (VSBV-1), forms a lineage separate from that of the known bornavirus species. (Funded by the Federal Ministry of Food and Agriculture [Germany] and others.).
Assuntos
Bornaviridae/genética , Encéfalo/patologia , Encefalite Viral/virologia , Infecções por Mononegavirales/virologia , Sciuridae/virologia , Idoso , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/líquido cefalorraquidiano , Bornaviridae/classificação , Bornaviridae/isolamento & purificação , Encefalite Viral/patologia , Evolução Fatal , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Infecções por Mononegavirales/patologia , Infecções por Mononegavirales/transmissão , Filogenia , RNA Viral , Análise de Sequência de DNA , Zoonoses/transmissão , Zoonoses/virologiaRESUMO
In 2013, an adult red squirrel (Sciurus vulgaris) was found dead showing, during necropsy, acute diffuse catarrhal enteritis. Using a virus screening pipeline based on a pan-viral DNA microarray with downstream next-generation sequencing a novel squirrel-associated virus was identified with mastadenovirus-typical sequence elements. Phylogenetic analysis of hexon protein amino acid sequences demonstrated the highest similarity to Equine adenovirus (AdV) 2, but a strong divergence to a squirrel AdV from Korea and other rodent AdVs. Shorter hexon gene segment investigations confirmed a close relationship with other squirrel-derived AdVs from Europe. The novel virus species was tentatively designated as Squirrel Adenovirus-1.
Assuntos
Infecções por Adenoviridae/veterinária , Adenoviridae/isolamento & purificação , DNA Viral/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Análise de Sequência com Séries de Oligonucleotídeos , Sciuridae/virologia , Adenoviridae/genética , Infecções por Adenoviridae/virologia , Animais , FilogeniaRESUMO
A brain sample of a Natterer's bat tested positive for rabies with classical virological techniques. Molecular techniques confirmed the presence of Bokeloh bat lyssavirus (BBLV) in Germany for the second time. Sequence analysis revealed a close genetic relationship to the initial German BBLV case. Using a TaqMan RT-PCR specific for BBLV viral RNA was detected in various other organs albeit with differences in the relative viral load.