Cysteine-cysteine chemokine receptor 5 (CCR5) profile of HIV-infected subjects attending University of Calabar Teaching Hospital, Calabar, Southern Nigeria.

BACKGROUND: Cysteine-cysteine chemokine receptor 5 is the main HIV co-receptor involved in the virus and cell-to-cell spread. A variant of the CCR5 gene known as CCR5-Δ32 which is a product of 32 base pair deletion in the gene plays critical role in the infection and progression to AIDS. The study was carried out to determine the CCR5 genotype of HIV-infected subjects attending University of Calabar Teaching Hospital, Calabar. METHODS: A total of 100 subjects attending HIV clinic, University of Calabar Teaching Hospital were purposively recruited for this study. DNA was extracted from each sample using the Quick gDNA miniprep DNA extraction kit, Zymo Research. Polymerase chain reaction (PCR) was used in the amplification of CCR5 gene in each DNA in a 9700 ABI Thermo cycler and then resolved on 4% agarose gel electrophoresis. RESULT: Out of the 100 samples assessed, 100 (100%) were homozygous for the CCR5 wild type gene (CCR5-wt), while none (0%) was homozygous for the CCR5-Δ32 (mutant type), and heterozygosity was not observed. CONCLUSION: This study observed absence of CCR5-Δ32 deletion gene among the studied subjects in Calabar, implying lack of genetic advantage in HIV infection and possible rapid progression towards AIDS if other precautions are not checked.

Prevalence of asymptomatic malaria in HIV-infected subjects on cotrimoxazole antimalarial prophylaxis attending a tertiary health care center in southern Nigeria: a cross-sectional study.

INTRODUCTION: Co-infection has become a major contributor to increased burden of morbidity and mortality in HIV infection. The aim of this study was to assess the prevalence of asymptomatic malaria in the HIV-infected subjects on antimalarial prophylaxis and provide information to improve management of HIV subjects. METHODS: This was a cross-sectional study with a purposive sampling. Microscopy method was used for the confirmation of malaria parasitemia status. The study was performed in University of Calabar Teaching Hospital, Calabar, Cross River State, Nigeria, a major tertiary health institution within the period of January to June 2016 involving 100 participants. RESULTS: The majority (65%) of the study participants were females. The majority of the studied population belonged to the age range 33-38 years old. Most (45%) of the patients had CD4 count ≥500 cells/µL. The prevalence of asymptomatic malaria was found to be 13% (13/100). The distribution of asymptomatic malaria based on gender and age were found not to be statistically significant (P>0.05). Subjects with CD4 count in the range of 200-499 cells/µL had the highest prevalence (24.39%) of asymptomatic malaria. CONCLUSIONS: Considering that all the studied participants were on antimalarial prophylaxis, it signals a public health concern to employ more intensive preventive methods in addition to antimalaria prophylaxis.