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1.
Sensors (Basel) ; 24(6)2024 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-38544065

RESUMO

Computer vision and optical technologies have become attractive alternatives for metrology, chemical analysis, and non-destructive structural health monitoring and the evaluation of aerospace, civil, and mechanical engineering structures [...].

2.
Matern Child Health J ; 27(9): 1607-1615, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37394617

RESUMO

OBJECTIVE: This cohort study was to investigate maternal knowledge of the oral health of woman and children during pregnancy and after childbirth, and the associated factors. METHODS: Groups of women participating in a public prenatal dental care programme in Brazil were assessed, in two different stages. In the first stage, pregnant women were assessed for their own oral health. In the second stage, after childbirth, women were assessed for the oral health of their child. The examiner assigned a maternal knowledge score to the questionnaires, considering the ideal alternatives within the context of oral health promotion as correct answers. Statistical analysis included the Kruskal-Wallis and multiple linear regression tests, considering a level of significance of P < 0.05. RESULTS: Ninety-eight women were included in the study, with a mean age 26.27 years (SD 6.51). In the regression analysis, the maternal knowledge score was associated with the presence of myths about oral health (P < 0.01), the children first dental appointment in the first year of life (P = 0.07), the presence of a non-nutritious sucking habit (P < 0.01), considering dental treatment to be important during pregnancy (P < 0.01), and having been instructed on oral health during pregnancy (P < 0.01) and after the children birth (P = 0.02). CONCLUSION: This study suggests that the women showed a regular score of knowledge about their oral health and that of their children, as they still believed in some myths about oral health and the risks of dental treatment during pregnancy. Women who received guidance on oral health in pregnancy and after birth showed higher knowledge of their oral health and that of their children, demonstrating the importance of carrying out health promotion actions during pregnancy and the first years of the child's life.


Assuntos
Saúde Bucal , Gestantes , Criança , Feminino , Gravidez , Humanos , Adulto , Estudos de Coortes , Parto , Mães
3.
J Mater Sci Mater Med ; 34(5): 21, 2023 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-37118367

RESUMO

High implant survival rates have been achieved in recent decades due to continual modifications in implant design and surface topography, however there is still an ongoing quest to control peri-implant bone loss. The objective of this work was to develop Ti-35Nb-7Zr-5Ta (TNZT) alloys, perform physicochemical and morphological characterization of their surface modified by electrolytic oxidative plasma technique with ions related to osseointegration and lastly evaluate bacterial colonization in vitro. Three groups were evaluated: C group (polished TNZT), CaP group (sodium ß glycerophosphate + calcium acetate) and Mg group (magnesium acetate). Before and after anodizing the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy with field emission gun (FEG-SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (DRX), wettability (goniometer) and roughness (rugometer). Controlled and treated specimens were contaminated with unstimulated saliva collected from 10 healthy volunteers. Then, biofilm samples were collected and up to 35 microbial species, including commensal and pathogenic microorganisms, were identified and quantified by the Checkerboard DNA-DNA Hybridization method. The CaP group modified the surface morphology in the form of pores, while the Mg group modified it in the form of flakes. The contact angle was significantly smaller in the CaP group. The average roughness was higher in the CaP and Mg groups. A smaller total amount of bacteria was identified in the Mg group and relevant differences were found in the microbial profile associated with different surface treatments. Therefore, considering the microbiological profile and for the prevention of peri-implantitis, the Mg group presented more satisfactory and encouraging results for the manufacture of dental implants.


Assuntos
Cálcio , Implantes Dentários , Humanos , Fosfatos , Magnésio , Ligas/química , Microscopia Eletrônica de Varredura , DNA , Titânio/química , Propriedades de Superfície
4.
Br J Nutr ; 127(8): 1143-1152, 2022 04 28.
Artigo em Inglês | MEDLINE | ID: mdl-34121648

RESUMO

Methionine is a precursor of s-adenosylmethionine, the main donor of methyl radicals for methylation of DNA and other compounds. Previous studies have shown that reduced availability of methyl radicals during pregnancy/lactation decreased offspring perigonadal white adipose tissue (PWAT) and body weight. Therefore, we aimed to evaluate the effects of methionine supplementation during early development, a time of great ontogenic plasticity, by assessing the biometric, biochemical and behavioural parameters of the offspring of adult Swiss female mice supplemented with 1 % methionine in water 1 month before pregnancy, during pregnancy or pregnancy/lactation. After birth, the offspring were distributed into three groups: control (CT), methionine supplementation during pregnancy (SP) and methionine supplementation during pregnancy and lactation (SPL), and were followed until postnatal day (PND) 300. No changes were observed in offspring birth weight in both sexes. At PND 5, 28 and 90, no differences in body weight were found in females; however, at PND 300, SP and SPL females showed an increase in body weight when compared with the control group. This increase in body weight was accompanied by a total and relative increase in PWAT, and a decrease in locomotor activity in these groups. No differences in the body and organ weights were found in male offspring. In conclusion, the increased availability of methyl radicals during pregnancy and lactation impacted long-term body composition and locomotor activity in female offspring.


Assuntos
Lactação , Metionina , Animais , Peso Corporal , Suplementos Nutricionais , Feminino , Locomoção , Masculino , Metionina/farmacologia , Camundongos , Gravidez
5.
Sensors (Basel) ; 22(11)2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35684834

RESUMO

In this paper, we describe an image-based approach for estimating the speed of a moving vessel using the wakes that remain on the surface of water after the vessel has passed. The proposed method calculates the speed of the vessel using only one RGB image. In this study, we used the vanishing line of the mean water plane, the camera height concerning the level of the tide, and the intrinsic parameters of the camera to perform geometric rectification on the surface plane of the water. We detected the location of troughs on one of the wake arms and computed the distance between them in the rectified image to estimate the speed of the vessel as a so-called inverse ship wake problem. We used a radar that was designed to monitor ships to validate the proposed method. We used statistical studies to determine the reliability and error propagation of the estimated values throughout the calculation process. The experiments showed that the proposed method produced precise and accurate results that agreed with the actual radar data when using a simple capture device, such as a conventional camera.


Assuntos
Radar , Reprodução , Intervalos de Confiança , Reprodutibilidade dos Testes , Água
6.
Sensors (Basel) ; 21(22)2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34833700

RESUMO

Submarines are considered extremely strategic for any naval army due to their stealth capability. Periscopes are crucial sensors for these vessels, and emerging to the surface or periscope depth is required to identify visual contacts through this device. This maneuver has many procedures and usually has to be fast and agile to avoid exposure. This paper presents and implements a novel architecture for real submarine periscopes developed for future Brazilian naval fleet operations. Our system consists of a probe that is connected to the craft and carries a 360 camera. We project and take the images inside the vessel using traditional VR/XR devices. We also propose and implement an efficient computer vision-based MR technique to estimate and display detected vessels effectively and precisely. The vessel detection model is trained using synthetic images. So, we built and made available a dataset composed of 99,000 images. Finally, we also estimate distances of the classified elements, showing all the information in an AR-based interface. Although the probe is wired-connected, it allows for the vessel to stand in deep positions, reducing its exposure and introducing a new way for submarine maneuvers and operations. We validate our proposal through a user experience experiment using 19 experts in periscope operations.


Assuntos
Realidade Aumentada , Brasil , Interface Usuário-Computador
7.
Dent Traumatol ; 34(2): 93-99, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29282852

RESUMO

BACKGROUND/AIMS: The prognosis of traumatized teeth can be optimized if appropriate procedures are following at the time of the accident. The aim of this study was to evaluate the level of knowledge of students' parents from a group of Brazilian schools regarding fracture and tooth avulsion in permanent teeth. METHODS: Parents of 802 students were interviewed using a pre-tested questionnaire designed to explore how they would manage their child's dental avulsion or fracture. RESULTS: Among the parents, 8.6% reported their child had already suffered tooth injuries. Concerning dental fracture, 49.5% considered the possibility of bonding the tooth fragment and, for dental avulsion, 65.5% considered tooth replantation. Approximately 33.5% of parents would take the child to the dental office in case of fracture or dental avulsion, and of those, 66.2% would do so immediately. Further, 69.5% (for fracture) and 70.4% (for avulsion) would do so regardless of symptoms; only 24.2% and 21.3%, respectively, would seek care only if there was pain. In case of avulsion, 22.3% of those interviewed would store the tooth in liquid, such as saline solution (17.8%), water (16.4%) and fresh milk (12.2%). CONCLUSION: Parents had partial and insufficient knowledge as to the proper approach to be adopted in cases of fracture and dental avulsion. Previous experience of dental trauma was not related to more appropriate behavior regarding these injuries.


Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Pais/psicologia , Traumatismos Dentários/epidemiologia , Traumatismos Dentários/terapia , Adolescente , Adulto , Brasil/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Prognóstico , Inquéritos e Questionários
8.
Lasers Med Sci ; 30(2): 677-83, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23912780

RESUMO

The aim of the present study was to evaluate the antimicrobial effect of antimicrobial photodynamic therapy (aPDT) in alveolar treatment of areas with induced periodontitis. Thirty male Wistar rats were subjected to ligature-induced periodontal disease (PD) in the first left inferior molars, while the right side molars did not receive ligatures. After 7 days of PD evolution, ligatures were removed from the left side, and the first left and right mandibular molars were extracted. Afterwards, animals were divided into groups according to the following treatments: control (C)--no treatment; mechanical debridement (MD)--mechanical debridement and irrigation with saline solution; and aPDT--mechanical debridement, irrigation with toluidine blue O (TBO), and 1 min of laser irradiation (GaAlAs, 660 nm, 30 mW, 32 J/cm(2), 60 s). Ligatures were removed and samples of the alveolar content after extraction and after each treatment were collected for microbial processing by real-time polymerase chain reaction with specific primers for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola. Data were submitted to statistical analysis by multiple comparison tests (McNemar test; p < 0.05). T. denticola was not found in the collected samples. A. actinomycetemcomitans and P. gingivalis were found in ligature samples. Tooth socket samples without periodontitis induction presented lesser microbial charge than samples with induced periodontitis (p < 0.05). aPDT significantly reduced A. actinomycetemcomitans levels on the left side (p < 0.05). It was concluded that aPDT was an effective antimicrobial treatment for tooth sockets in areas affected by induced periodontitis.


Assuntos
Dente Molar/microbiologia , Periodontite/tratamento farmacológico , Fotoquimioterapia/métodos , Alvéolo Dental/microbiologia , Aggregatibacter actinomycetemcomitans , Animais , Lasers , Masculino , Porphyromonas gingivalis , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Cloreto de Tolônio
9.
Int J Dent ; 2024: 5945033, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38957372

RESUMO

Objective: To evaluate the solubility and the volumetric and morphological alterations of bioceramic filling material (Bio-CP) for primary teeth. Materials and Methods: Bio-CP, Calen thickened with zinc oxide (Calen-ZO), and with zinc oxide eugenol (ZOE) were placed in 1- or 2-mm-diameter polyethylene tubes and immersed in water or phosphate-buffered saline (PBS) for 30 days. The solubility (mass loss) was assessed using methodology modified from ISO 6876. Filling capacity, volumetric changes, and presence of voids were assessed by microcomputed tomography (micro-CT). The surface distribution of the chemical elements and the crystalline phases was evaluated by energy scattering X-ray scanning electron microscopy (SEM-EDX) and X-ray diffraction (XRD) to detect hydroxyapatite precipitate and components. The Shapiro-Wilk, Kruskal-Wallis, and Dunn's or two-way ANOVA and Tukey post hoc test were used (α = 0.05). Results: The solubility was ZOE > Calen-ZO = Bio-CP. Calen-ZO and Bio-CP were more soluble in water than in PBS. All the materials showed greater solubility in 2-mm tube diameter in both PBS and distilled water, except for Bio-CP in distilled water, which showed no difference between both tube diameters (1 and 2 mm). Only Calen-ZO and ZOE were analyzed by micro-CT, because Bio-CP separated into two phases during scanning. Calen-ZO had greater volumetric loss and presence of voids than ZOE in water, but there was no difference in PBS. The hydroxyapatite precipitate on the surface of Bio-CP and Calen-ZO was detected after immersion in PBS. Conclusion: Although Bio-CP had acceptable solubility and filling capacity, its composition did not allow a proper volumetric and void assessment. From a clinical perspective, Bio-CP has the potential to become a suitable material for root canal filling in primary teeth. Nonetheless, its composition must first be revised to achieve better chemical stability prior to its recommendation.

10.
Lasers Med Sci ; 28(2): 407-14, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22370617

RESUMO

The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm(2) on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Regeneração Óssea/efeitos da radiação , Difosfonatos/farmacologia , Terapia com Luz de Baixa Intensidade/métodos , Crânio/efeitos da radiação , Animais , Conservadores da Densidade Óssea/farmacologia , Doenças Ósseas Metabólicas/cirurgia , Interpretação Estatística de Dados , Feminino , Processamento de Imagem Assistida por Computador , Ovariectomia , Ratos , Ratos Wistar , Crânio/efeitos dos fármacos , Crânio/patologia , Crânio/cirurgia
11.
Lasers Med Sci ; 28(1): 349-52, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22825319

RESUMO

Low-level laser (LLL) has been used on peri-implant tissues for accelerating bone formation. However, the effect of one session of LLL in the strength of bone-implant interface during early healing process remains unclear. The present study aims to evaluate the removal torque of titanium implants irradiated with LLL during surgical preparation of implant bed, in comparison to non-irradiation. Sixty-four Wistar rats were used. Half of the animals were included in LLL group, while the other half remained as control. All animals had the tibia prepared with a 2 mm drill, and a titanium implant (2.2 × 4 mm) was inserted. Animals from LLL group were irradiated with laser (gallium aluminum arsenide), with a wavelength of 808 nm, a measured power output of 50 mW, to emit radiation in collimated beams (0.4 cm(2)), for 1 min and 23 s, and an energy density of 11 J/cm(2). Two applications (22 J/cm(2)) were performed immediately after bed preparation for implant installation. Flaps were sutured, and animals from both groups were sacrificed 7, 15, 30, and 45 days after implant installation, when load necessary for removing implant from bone was evaluated by using a torquimeter. In both groups, torque values tended to increase overtime; and at 30 and 45 days periods, values were statistically higher for LLL group in comparison to control (ANOVA test, p < 0.0001). Thus, it could be suggested that a single session of irradiation with LLL was beneficial to improve bone-implant interface strength, contributing to the osseointegration process.


Assuntos
Implantação Dentária Endóssea/métodos , Implantes Dentários , Terapia com Luz de Baixa Intensidade/métodos , Osseointegração/efeitos da radiação , Análise de Variância , Animais , Fenômenos Biomecânicos , Modelos Animais , Osteogênese/efeitos da radiação , Ratos , Ratos Wistar , Retalhos Cirúrgicos , Tíbia , Titânio , Torque , Cicatrização/efeitos da radiação
12.
Lasers Med Sci ; 28(1): 143-50, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22526974

RESUMO

The aim of this study was to histologically and histometrically evaluate the influence of repeated adjunctive antimicrobial photodynamic therapy (aPDT) on bone loss (BL) in furcation areas in rats. Periodontitis was induced by placing a ligature around the mandibular molar in 75 rats. The animals were divided into five groups: the SS group was treated with saline solution (SS); the SRP group received scaling and root planing (SRP); the aPDT1 group received SRP as well as toluidine blue (TBO) and low-level laser therapy (LLLT; InGaAlP, 660 nm; 4.94 J/cm(2)/point) postoperatively at 0 h; the aPDT2 group received SRP as well as TBO and LLLT postoperatively at 0, 24, 28, and 72 h; and the aPDT3 group received SRP, TBO, and LLLT postoperatively at 0, 48, 96, and 144 h. The area of BL in the furcation region of the molar was histometrically analyzed. Data were analyzed statistically (P < 0.05). Animals treated with a single episode of aPDT showed less BL at days 7 and 30 than those who received only SRP treatment. No significant differences were found among the aPDT groups (P > 0.05). Repeated aPDT did not improve BL reduction when compared to a single episode of aPDT.


Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Periodontite/radioterapia , Perda do Osso Alveolar , Análise de Variância , Animais , Raspagem Dentária , Masculino , Periodontite/microbiologia , Ratos , Ratos Wistar , Aplainamento Radicular , Cloreto de Sódio/farmacologia , Cloreto de Tolônio/farmacologia
13.
Polymers (Basel) ; 15(24)2023 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-38139901

RESUMO

Electronic devices are sensitive to electromagnetic (EM) emissions, and require electromagnetic shielding protection to ensure good operation, and prevent noise, malfunctioning, or even burning. To ensure protection, it is important to develop suitable material and design solutions for electronic enclosures. Most common enclosures are made with metal alloys using traditional manufacturing methods. However, using thermoplastic composites combined with additive manufacturing (AM) technologies emerges as an alternative that enables the fabrication of complex parts that are lightweight, consolidated, and oxidation- and corrosion-resistant. In this research, an AM technique based on material extrusion was used to print 2 mm-thick specimens with a multi-material made of micro-carbon fiber (CF)-filled polyamide that was reinforced at specific layers using continuous carbon fibers stacked with a 90° rotation to each other. The specimens' electromagnetic shielding effectiveness (EMSE) was evaluated in the frequency band of 0.03-3 GHz using the coaxial transmission line method. Depending on the number of CF layers, the EM shielding obtained can be up to 70 dB, with a specific shielding up to 60 dB.cm3/g, predominantly by the absorption mechanism, being 22 times higher than without the CF layers. These findings promote this innovative approach to lightweight customizable solutions for EM shielding applications.

14.
J Periodontal Implant Sci ; 52(1): 39-53, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35187872

RESUMO

PURPOSE: This study evaluated the systemic and local effects of doxycycline (DOX) and low-intensity laser (LIL) treatment as adjuvants to scaling and root planing (SRP) in the treatment of experimental periodontitis in rats. METHODS: The sample consisted of 180 male rats (Rattus norvegicus albinus, Wistar), of which 30 did not receive induction of periodontal disease (negative control [NC] group) and 150 received induction of periodontal disease in the lower first molar. After 7 days, the ligature was removed, and the animals were divided into the following groups: NT (no treatment), SRP (SRP), DOX (SRP and DOX irrigation), LIL (SRP and laser irradiation), and DOX+LIL (SRP, DOX, and LIL). The animals were euthanized at 7, 15, and 30 days; thereafter, biochemical, radiographic, histological, and immunohistochemical analyses were performed. RESULTS: In the intragroup analysis, lower concentrations of α-1-glycoprotein acid (α-1-Ga) and complement 3 (C3) were observed in the DOX+LIL group than in all other groups at all time points, as well as lower levels of complement 4 (C4) at 15 and 30 days (P<0.001). Less bone loss was observed in the DOX, LIL, and DOX+LIL groups than in the NC and SRP groups at all time points (P<0.001). There was a smaller number of tartrate-resistant acid phosphatase (TRAP)-positive cells in the DOX+LIL group than in the other groups at all time points (P<0.001). Positive correlations were observed between the systemic levels of α-1-Ga, C3, and C4 and the number of TRAP-positive cells. CONCLUSIONS: The combination of DOX with LIL as SRP adjuvants was effective both systemically and locally for the treatment of experimental periodontitis in rats.

15.
J Clin Periodontol ; 38(12): 1106-14, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22092666

RESUMO

BACKGROUND: The aim of this study was to compare antimicrobial photodynamic therapy (aPDT) as an adjunctive treatment to scaling and root planing (SRP) for induced periodontitis in nicotine-modified rats. MATERIAL & METHODS: A total of 240 rats were evenly divided into two groups: C - saline solution treatment; N - nicotine treatment. Periodontal disease was induced in both groups at the first mandibular molar. After 7 days, the ligature was removed. All animals were submitted to SRP and were divided according to the following treatments: SRP - irrigation with saline solution; Toluidine Blue-O (TBO) - irrigation with phenothiazinium dye (100 µg/ml); LLLT - laser irradiation (660 nm; 0.03 W; 4 J); and aPDT - TBO and laser irradiation. Ten animals in each group/treatment were euthanized at 7, 15 and 30 days. The histometric and immunohistochemical values were statistically analysed. RESULTS: Intragroup analysis demonstrated that in both groups the aPDT treatment resulted in lower bone loss (BL) when compared to SRP in all experimental periods. Intergroup analysis demonstrated that aPDT treatment resulted in lower BL in Group N than in Group C treated with SRP in all experimental periods. CONCLUSION: Antimicrobial photodynamic therapy was an effective adjunctive treatment to SRP for induced periodontitis in nicotine-modified rats.


Assuntos
Profilaxia Dentária/métodos , Imunossupressores/efeitos adversos , Nicotina/efeitos adversos , Periodontite/terapia , Fotoquimioterapia/métodos , Perda do Osso Alveolar/induzido quimicamente , Perda do Osso Alveolar/metabolismo , Perda do Osso Alveolar/terapia , Animais , Bactérias/efeitos da radiação , Terapia Combinada , Modelos Animais de Doenças , Imuno-Histoquímica , Masculino , Mandíbula , Dente Molar , Osteoprotegerina/metabolismo , Periodontite/induzido quimicamente , Periodontite/microbiologia , Fármacos Fotossensibilizantes/uso terapêutico , Ligante RANK/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar
16.
J Craniofac Surg ; 22(6): 2011-6, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22067857

RESUMO

The objective of the current study was to assess histomorphometrically the healing process of recession defects associated with scraped roots treated with subepithelial connective tissue graft (SCTG). Six dogs were used. Bone dehiscence defects (6 × 8 mm) and root planing were carried out on maxillary canine teeth. Following a split-mouth model, according to the treatment, left canines (control) were covered with coronally positioned flap (CPF). Right canines were submitted to treatments with SCTG. After a 3-month postoperative period, the animals were killed, and the blocks processed for the histomorphometric assessment. Data assessment demonstrated that the CPF group showed parameters of a new connective tissue attachment, length of new cement (NC), length of new bone (mean ± SD: 0.95 ± 0.53, 2.44 ± 1.97, and 1.96 ± 2.29 mm, respectively), which were higher than those of SCTG group (mean ± SD: 0.71 ± 0.36, 2.21 ± 1.28, and 1.52 ± 1.31 mm, respectively), although not significantly (P > 0.05). The length of both epithelial tissue and connective tissue apposition in the SCTG group (mean ± SD: 1.70 ± 0.53 and 2.62 ± 1.52 mm, respectively) were higher than those of the CPF group (mean ± SD: 1.18 ± 0.49 and 2.03 ± 1.03 mm, respectively), although showing no significant differences (P > 0.05). Within the limits of the current study, it was possible to conclude that there were no significant differences between the groups according to the histologic parameters assessed.


Assuntos
Tecido Conjuntivo/transplante , Retração Gengival/cirurgia , Gengivoplastia/métodos , Aplainamento Radicular , Retalhos Cirúrgicos , Animais , Dente Canino , Cães , Masculino
17.
Steroids ; 174: 108899, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34358557

RESUMO

Our aim was to evaluate the independent and associated effects of nandrolone decanoate (DECA) and resistance exercise (REx) on central and peripheral hormones and neuropeptides related to energy balance in male rats. The experimental protocol was performed for eight weeks and comprised four groups: control (C) - exposed to vehicle 3x/wk; trained (T) - REx 5x/wk and vehicle 3x/wk; decanoate (D) - exposed to DECA (5 mg/kg) 3x/wk, and REx with DECA (TD) - submitted to REx 5x/wk and DECA (5 mg/kg) 3x/wk. Cross-sectional area analysis of the gastrocnemius muscle was higher in the T and TD groups compared to the C group. Biometrical analyses showed a decrease in body weight only in the TD compared to the C group, however, a reduction in total fat mass was observed in both the T and TD when compared to the C group. In respect of hypothalamic mRNA expression, there was an increase in prepro-orexin in the T compared to the C group. In mesenteric fat there was a decrease in leptin expression in the T and TD compared to the C group. Plasma evaluations showed reduced leptin concentrations in D, T and TD compared to C, and an increase in orexin-A in the D group compared to the C and T groups. Our data showed that REx was related to central and peripheral changes in energy metabolism, while DECA changed only peripheral components. REx associated with DECA promoted peripheral changes in energy metabolism and decreased body and fat weights.


Assuntos
Decanoato de Nandrolona
18.
Photochem Photobiol ; 97(5): 1104-1115, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33713346

RESUMO

This study evaluated the influence of Doxycycline (DOX) and Low-Intensity Laser (LIL) (InGaAlP diode laser) as scaling and root planing (SRP) adjuvants in the treatment of periodontitis induced in rats. The rats received periodontal disease induction, and after 7 days, the ligature was removed, and the animals were divided into groups/treatments: NT-receive no treatment; SRP-submitted only to SRP; DOX-submitted to SRP and DOX irrigation; LIL-submitted to SRP and LIL irradiation; and DOX + LIL-submitted to SRP treatments, DOX irrigation and LIL irradiation. The animals were sacrificed at 7, 15 and 30 days, and then, the analyses were performed. A lower concentration of Alpha-glycoprotein acid and Complement 3 was observed in the DOX + LIL group compared to all the other groups in all the periods, and for Complement 4 at 15 and 30 days (P < 0.01). A lower bone loss (BL) was observed in the DOX + LIL group compared to all the other groups in all the periods (P < 0.01). It can be concluded that LIL was effective in the reduction of proteins, and its association with DOX was effective in the reduction of proteins and BL, in the treatment of periodontal induction in rats.


Assuntos
Lasers Semicondutores , Periodontite , Animais , Terapia Combinada , Doxiciclina/farmacologia , Doxiciclina/uso terapêutico , Lasers Semicondutores/uso terapêutico , Periodontite/tratamento farmacológico , Ratos , Ratos Wistar , Aplainamento Radicular
19.
Lasers Med Sci ; 25(2): 221-8, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19533211

RESUMO

The aim of this study was to conduct a histological assessment of the effect of photodynamic therapy (PDT) on the repairing of third-degree-burn wounds made on the backs of rats with a heated scalpel. Ninety-six rats were divided into groups: G1, control (n = 24), cold scalpel; G2, burned, heated scalpel (n = 24); G3, low-level laser therapy (LLLT) (n = 24), on burns; and G4, photodynamic therapy (PDT) (n = 24), toluidine-O blue (100 microg/ml) and LLLT treatment on burns. The laser (685 nm) was applied in continuous mode, 50 mW, 4.5 J/cm(2), contact mode at nine points (9 s/point). Eight animals in each group were killed at 3 days, 7 days or 14 days after surgery, and tissue specimens containing the whole wounded area were removed and processed for histological analysis; the results were statistically analyzed with Kruskal-Wallis and Dunn's tests (P < 0.05). The results demonstrated significant differences between G2 and G3, and between G2 and G4, at both 3 days and 7 days, with regard to acute inflammation scores; G1 and G2 showed significant differences when compared with G4 at 3 days, with regard to neo-angiogenesis scores; G1 and G2 were statistically different from G3 and G4 at both 3 days and 7 days, with regard to re-epithelization scores; G2 showed statistically significant differences when compared with G3 and G4 with regard to collagen fiber scores at 7 days. LLLT and PDT acted as a biostimulating coadjuvant agent, balancing the undesirable effect of the burn on the wound healing process, acting mainly in the early healing stages, hastening inflammation and increasing collagen deposition.


Assuntos
Queimaduras/tratamento farmacológico , Fotoquimioterapia , Cicatrização/efeitos dos fármacos , Animais , Queimaduras/patologia , Queimaduras/radioterapia , Terapia com Luz de Baixa Intensidade , Masculino , Fármacos Fotossensibilizantes/uso terapêutico , Ratos , Ratos Wistar , Cloreto de Tolônio/uso terapêutico , Cicatrização/efeitos da radiação
20.
Lasers Med Sci ; 25(2): 197-206, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19440786

RESUMO

The aim of this study was to compare low-level laser therapy (LLLT) as adjuvant treatment for induced periodontitis with scaling and root planing (SRP) in dexamethasone-treated rats. One-hundred twenty rats were divided into groups: D group (n = 60), treated with dexamethasone; ND group (n = 60) treated with saline solution. In both groups, periodontal disease was induced by ligature at the left first mandibular molar. After 7 days, the ligature was removed and all animals were subjected to SRP and were divided according to the following treatments: SRP, irrigation with saline solution (SS); SRP + LLLT, SS and laser irradiation (660 nm; 24 J; 0.428 W/cm(2)). Ten animals in each treatment were killed after 7 days, 15 days and 30 days. The radiographic and histometric values were statistically analyzed. In all groups radiographic and histometric analysis showed less bone loss (P < 0.05) in animals treated with SRP + LLLT in all experimental periods. SRP + LLLT was an effective adjuvant conventional treatment for periodontitis in rats treated with dexamethasone.


Assuntos
Terapia com Luz de Baixa Intensidade , Periodontite/radioterapia , Perda do Osso Alveolar/patologia , Perda do Osso Alveolar/radioterapia , Perda do Osso Alveolar/terapia , Animais , Terapia Combinada , Raspagem Dentária , Dexametasona/toxicidade , Masculino , Periodontite/etiologia , Periodontite/patologia , Periodontite/terapia , Ratos , Ratos Wistar , Aplainamento Radicular
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