Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 116
Filtrar
1.
Ren Fail ; 45(1): 2220418, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37288756

RESUMO

Our research explores the role of M1 macrophage polarization in endothelium-to-myofibroblast transition (EndMT) and chronic allograft dysfunction (CAD). GSE21374 transcriptome sequencing data were obtained. Transplanted nephrectomy specimens from CAD patients were collected and studied to explore the infiltration of M1 and M2 macrophages using immunofluorescence, PCR, and Western blotting (WB). A co-culture model of M1 macrophages, polarized from mouse bone marrow-derived macrophages (BMDM) or Raw264.7, and aortic endothelial cells was established, and EndMT was tested using PCR and WB. RNA-sequencing was performed on the macrophages from the mouse BMDM. The TNF-α secreted from the polarized M1 macrophages was verified using ELISA. Based on the GEO public database, it was observed that macrophages were significantly infiltrated in CAD allograft tissues, with CD68(+) iNOS(+) M1 macrophages significantly infiltrating the glomeruli of allograft tissues, and CD68(+)CD206(+) M2 macrophages notably infiltrating the allograft interstitial area. The mRNA expression of the M1 macrophage marker inducible nitric oxide synthase (iNOS) was significantly increased (p < 0.05) and M1 macrophages were found to significantly promote the EndMT process in vitro. RNA-Sequencing analysis revealed that TNF signaling could be involved in the EndMT induced by M1 macrophages, and in vitro studies confirmed that TNF-α in the supernatant was significantly higher. The renal allograft tissues of CAD patients were found to be significantly infiltrated by M1 macrophages and could promote the progression of CAD by secreting the cytokine TNF-α to induce EndMT in endothelial cells.


Assuntos
Transplante de Rim , Fator de Necrose Tumoral alfa , Camundongos , Animais , Fator de Necrose Tumoral alfa/metabolismo , Transplante de Rim/efeitos adversos , Células Endoteliais/metabolismo , Miofibroblastos/metabolismo , Macrófagos/metabolismo , Aloenxertos , Endotélio/metabolismo , RNA/metabolismo
2.
Ren Fail ; 45(1): 2210231, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37183797

RESUMO

BACKGROUND: The assessment and prevention of mineral and bone disorder (MBD) in kidney transplant recipients (KTRs) have not been standardized. This study aimed to evaluate MBD one year after kidney transplantation (KT) and identify the influencing factors of MBD. METHODS: A total of 95 KTRs in our center were enrolled. The changes in bone mineral density (BMD) and bone metabolism biochemical markers, including serum calcium (Ca), phosphorus(P), 25-hydroxyvitamin D(25(OH)vitD), intact parathyroid hormone (iPTH), bone alkaline phosphatase, osteocalcin (OC), type I collagen N-terminal peptide and type I collagen C-terminal peptide (CTx), over one year after KT were assessed. The possible influencing factors of BMD were analyzed. The relationships between bone metabolism biochemical markers were evaluated. The indicators between groups with or without iPTH normalization were also compared. RESULTS: MBD after KT was manifested as an increased prevalence of hypophosphatemia and bone loss, persistent 25(OH)vitD deficiency, and partially decreased PTH and bone turnover markers (BTMs). Femoral neck BMD was positively correlated with body mass index (BMI) and postoperative 25(OH)vitD, and negatively correlated with postoperative PTH. Lumbar spine BMD was positively correlated with BMI and preoperative TG, and negatively correlated with preoperative OC and CTx. BMD loss was positively associated with glucocorticoid accumulation. Preoperative and postoperative iPTH was negatively correlated with postoperative serum P and 25(OH)vitD, and positively correlated with postoperative Ca and BTMs. The recipients without iPTH normalization, who accounted for 41.0% of all KTRs, presented with higher Ca, lower P, higher BTMs, advanced age, and a higher prevalence of preoperative parathyroid hyperplasia. CONCLUSIONS: MBD persisted after KT, showing a close relationship with hyperparathyroidism, high bone turnover, and glucocorticoid accumulation.


Assuntos
Distúrbio Mineral e Ósseo na Doença Renal Crônica , Hiperparatireoidismo , Transplante de Rim , Humanos , Biomarcadores , Densidade Óssea , Remodelação Óssea , Estudos de Coortes , Colágeno Tipo I , Glucocorticoides , Transplante de Rim/efeitos adversos , Hormônio Paratireóideo , Peptídeos , Osteoporose
3.
Int Orthop ; 46(9): 2019-2028, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35616654

RESUMO

PURPOSE: We developed an augmentation technique for PCL reconstruction with independent internal brace reinforcement and evaluated the functional outcome after PCL reconstruction employing autologous hamstrings augmented with an internal brace system for patients with isolated or combined grade 3 posterior instability who were treated with this technique. METHODS: From January 2016 to January 2018, patients with isolated or combined grade 3 PCL tears who underwent single-bundle PCL reconstruction using autologous hamstrings augmented with independent internal braces were studied. The function of the operated knee was evaluated according to the International Knee Documentation Committee (IKDC) score, Lysholm score, and Tegner activity score. The patients were asked the level of returned to their previous sport. Posterior knee laxity was examined with a KT-1000 arthrometer, and data on range of motion (ROM), re-operation, and other complications were collected. RESULTS: A total of 33 consecutive patients who received single-bundle PCL reconstruction using autologous hamstrings augmented with independent internal braces with a minimum two years follow-up were included in this study. Two patients had undergone this procedure during the study period and were not included in this study (one had combined bone fractures, and one patient had previous meniscus surgery). Thirty-one patients were available for final analysis. The mean follow-up was 45.35 ± 10.88 months (range 29-66 months). The average IKDC subjective knee evaluation scores from 51.65 ± 12.35 to 84.52 ± 6.42, the Lysholm score from 53.90 ± 11.86 to 85.68 ± 4.99, and the Tegner score from 2.81 ± 0.79 to 6.71 ± 1.83 (P < 0.05 for all). The mean total posterior side-to-side difference in knee laxity, assessed using a KT-1000 arthrometer, decreased from 12.13 ± 2.66 mm pre-operatively to 1.87 ± 0.56 mm post-operatively at 70° (P < 0.05). Most patients (29/31) had normal or near normal knee ROM post-operatively; two patients revealed a 6-15° loss of knee flexion compared with the contralateral knee. Twenty-nine patients (93.55%) returned to a normal daily exercise level. Twenty-three patients (74.19%) returned to competitive sports with high-level sports (Tegner score of 6 or above; eleven patients (35.48%) reported to be on the same level as well as the Tegner level); six patients (19.35%) returned to recreational sports (Tegner score of 4 or 5). Two patients had Tegner scores of 2 and 3, indicating poor function level. No patient needed PCL revision surgery during the follow-up period. CONCLUSION: Single-bundle PCL reconstruction with internal brace augmentation for PCL injury exhibited satisfactory posterior stability and clinical outcomes in patients with isolated or combined grade 3 PCL injuries at a minimum two year follow-up.


Assuntos
Instabilidade Articular , Traumatismos do Joelho , Reconstrução do Ligamento Cruzado Posterior , Ligamento Cruzado Posterior , Seguimentos , Humanos , Instabilidade Articular/etiologia , Instabilidade Articular/cirurgia , Traumatismos do Joelho/cirurgia , Articulação do Joelho/cirurgia , Ligamento Cruzado Posterior/lesões , Ligamento Cruzado Posterior/cirurgia , Resultado do Tratamento
4.
Zhongguo Zhong Yao Za Zhi ; 47(20): 5610-5616, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36471979

RESUMO

This study aims to investigate the effect of Chaihu Shugan Powder(CHSG) on liver injury in rats with intrahepatic cholestasis by regulating farnesoid X receptor(FXR)/nuclear factor erythroid-2-related factor(Nrf2)/antioxidant response element(ARE) pathway. Eighty-four SD rats were classified into normal group, model group, CHSG-L group(0.5 g·kg~(-1)), CHSG-H group(2.5 g·kg~(-1)), ursodeoxycholic acid group(UDCA group, 100 mg·kg~(-1)), CHSG-H+sh-NC group(2.5 g·kg~(-1) CHSG+subcutaneous injection of sh-NC lentivirus), CHSG-H+sh-FXR group(2.5 g·kg~(-1) CHSG+subcutaneous injection of sh-FXR lentivirus), with 12 rats in each group. Rats were treated with corresponding drugs except for the normal group and the model group, once a day, for 7 days. On 5 th day, rats, except the normal group, were given α-naphthalene isothiocyanate(ANIT) at a dose of 100 mg·kg~(-1), once a day for 3 days to induce intrahepatic cholestasis, and the normal group was given the same amount of normal saline. Rats were anesthetized 1 h after the last administration and the 2 h bile flow was measured. Aeroset chemistry analyzer was employed to detect the levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bilirubin(TBIL), and total bile acid(TBA) in rat serum. Based on hematoxylin and eosin(HE) staining, the pathological changes of rat liver tissue were observed. Glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), and malondialdehyde(MDA) in rat liver tissue homogenate were monitored with corresponding kits. Western blot was used to detect the expression of FXR, Nrf2, and heme oxygenase-1(HO-1) proteins in rat liver tissue. Compared with the normal group, the model group showed many spots or concentrated necrotic areas in the liver tissue, infiltration of a large number of inflammatory cells, swelling liver cells with nuclear shrinkage. The 2 h bile flow, levels of GSH-Px and SOD, and relative expression of FXR, Nrf2, and HO-1 proteins were significantly lower, and the levels of ALT, AST, TBIL, TBA and MDA were significantly higher in the model group than in the normal group. Compared with the model group, CHSG-L group, CHSG-H group, and UDCA group demonstrated significant alleviation of pathological damage of the liver tissue, significantly high 2 h bile flow, levels of GSH-Px and SOD, and expression of FXR, Nrf2 and HO-1 proteins, and significantly low levels of ALT, AST, TBIL, TBA and MDA. Compared with the CHSG-H group, the CHSG-H+sh-FXR group had worse liver pathological damage, significantly low levels of 2 h bile flow, levels of GSH-Px and SOD, and expression of FXR, Nrf2, and HO-1 proteins, and significantly high levels of ALT, AST, TBIL, TBA, and MDA. CHSG may protect against liver injury in rats with intrahepatic cholestasis by activating the FXR/Nrf2/ARE pathway.


Assuntos
1-Naftilisotiocianato , Colestase Intra-Hepática , Ratos , Animais , 1-Naftilisotiocianato/toxicidade , Pós , Fator 2 Relacionado a NF-E2/genética , Ratos Sprague-Dawley , Colestase Intra-Hepática/tratamento farmacológico , Fígado , Superóxido Dismutase , Estresse Oxidativo
5.
Opt Express ; 27(4): 4329-4337, 2019 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-30876049

RESUMO

This work demonstrates thermal regeneration of gratings inscribed in a new type of multi-material glass-based photosensitive fiber. And isothermal annealing procedure has been carried out on a type-I seed grating (SG) imprinted in erbium-doped zirconia-yttria-alumina-germanium (Er-ZYAG) silica glass-based fiber, which is initiated from room temperature of 25°C up to 900°C. The findings show that the created regenerated grating (RG) has an ultrahigh thermal regeneration ratio with a value of 0.72.

6.
Appl Opt ; 57(9): 2050-2056, 2018 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-29603992

RESUMO

A Mach-Zehnder interferometric magnetic field sensor based on a photonic crystal fiber (PCF) and magnetic fluid (MF) was designed and experimentally demonstrated. The sensing probe consists of a single-mode-(SM)-multimode-PCF-SM fiber structure through arc fusion splicing. It was then laser engrave notched with the femtosecond laser so that the PCF cladding was selectively infilled MF. A well-defined interference pattern was obtained on account of the tunable refractive index of the MF infilled PCF cladding. The transmission spectra of the proposed sensor under different magnetic field intensities have been measured and theoretically analyzed. The results show that the sensitivity of the proposed sensor can reach -0.13 dB/mT and 0.07334 nm/mT in the magnetic field intensity from 1 mT to 20 mT and 2 mT to 20 mT, respectively.

7.
Opt Express ; 23(1): 452-63, 2015 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-25835690

RESUMO

In this work, we have demonstrated for the first time grating regeneration in hydrogenated fibers by direct CO(2) laser annealing. During the annealing process, the center wavelength redshifts as the intensity of the focused CO(2) laser on the grating is elevated. The reflectivity of the grating begins to decay as the temperature induced in the grating approaches the erasure temperature. The grating is completely erased and regenerated afterwards. The observed spectral results have provided the proof of occurrence of dehydroxylation and stress relaxation in the fiber core during the annealing process. Regenerated gratings with low loss, good temperature sensitivities and sustainability have been successfully developed by this technique.

8.
Opt Lett ; 40(5): 748-51, 2015 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-25723423

RESUMO

In this work, we have demonstrated thermal stress relaxation in regenerated fiber Bragg gratings (RFBGs) by using direct CO2-laser annealing technique. After the isothermal annealing and slow cooling process, the Bragg wavelength of the RFBG has been red-shifted. This modification is reversible by re-annealing and rapid cooling. It is repeatable with different cooling process in the subsequent annealing treatments. This phenomenon can be attributed to the thermal stress modification in the fiber core by means of manipulation of glass transition temperature with different cooling rates. This finding in this investigation is important for accurate temperature measurement of RFBG in dynamic environment.

9.
Cell Mol Neurobiol ; 35(5): 679-87, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25662849

RESUMO

Malignant gliomas are the most common primary brain tumors, and the molecular mechanisms involving their progression and recurrence are still largely unclear. Substantial data indicate that the oncogene miR-494-3p is significantly elevated in gliomas, but the molecular functions of miR-494-3p in gliomagenesis are largely unknown. The present study aimed to explore the role of miR-494-3p and its molecular mechanism in human brain gliomas, malignant glioma cell lines, and cancer stem-like cells. The expression level of miR-494-3p in 48 human glioma issues and 8 normal brain tissues was determined using stem-loop real-time polymerase chain reaction (PCR). To study the function of miR-494-3p inhibitor in glioma cells, the miR-494-3p inhibitor lentivirus was used to transfect glioma cells. Transwell invasion system was used to estimate the effects of miR-494-3p inhibitor on the invasiveness of glioma cells. A mouse model was used to test the effect of miR-494-3p inhibitor on glioma proliferation and invasion in vivo. Results showed that the expression of miR-494-3p in human brain glioma tissues was higher than in normal brain tissues. Downregulated expression of miR-494-3p can inhibit the invasion and proliferation and promote apoptosis in glioma cells. Quantitative reverse transcription PCR and Western blotting analysis revealed that the expression of PTEN was increased after downexpression of miR-494-3p in glioma cells (U87 and U251). miR-494-3p inhibitor could prevent migration, invasion, proliferation, and promote apotosis in gliomas through PTEN/AKT pathway. Therefore, the study results have shown that miR-494-3p may act as a therapeutic target in gliomas.


Assuntos
Apoptose , Movimento Celular , Glioblastoma/genética , Glioblastoma/patologia , MicroRNAs/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Regulação Neoplásica da Expressão Gênica , Glioblastoma/enzimologia , Humanos , Lentivirus/metabolismo , Masculino , Camundongos Nus , MicroRNAs/genética , Pessoa de Meia-Idade , Invasividade Neoplásica , PTEN Fosfo-Hidrolase/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais , Transfecção , Ensaios Antitumorais Modelo de Xenoenxerto
10.
Opt Lett ; 39(22): 6438-41, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-25490488

RESUMO

The work demonstrates for the first time a thermal regenerated grating (RG) operating at an ultra-high temperature up to 1400°C. A new class of photosensitive optical fiber based on erbium-doped yttrium stabilized zirconia-calcium-alumina-phospho silica (Er-YZCAPS) glass is fabricated using modified chemical vapor deposition (MCVD) process, followed by solution doping technique and conventional fiber drawing. A type-I seed grating inscribed in this fiber is thermal regenerated based on the conventional thermal annealing technique. The investigation result indicates that the produced RG has an ultrahigh temperature sustainability up to 1400°C. The measured temperature sensitivities are 14.1 and 15.1 pm/°C for the temperature ranges of 25°C-1000°C and 1000°C-1400°C, respectively.

11.
Clin Anat ; 27(4): 631-6, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-23526687

RESUMO

The aim of this study was to investigate the occurrence and patterns of the communicating branches of cords of the brachial plexus (BPs). This study was performed with 50 fixed adult cadavers (all 100 sides). The BPs were exposed, the presence of the communicating branches of BPs were determined, measured, and photographed. The communicating branches were identified in 27 sides of the BPs. According to enthesis, the communicating branches between the medial and lateral cords (25 sides) were divided into five types. The most common branches connected the lateral cord with the medial root of the median nerve (16 sides). All the communicating branches between the lateral and medial cords obliquely crossed anterior to the axillary artery and passed below the thoracoacromial artery trunk. The distance of the communicating branch with the origin of thoracoacromial artery trunk was 1.60 ± 0.64 cm. The length, transverse diameter, and anteroposterior diameter of communicating branch were 1.67 ± 0.62 cm, 1.77 ± 0.63 mm, and 1.91 ± 0.34 mm, respectively. These anatomical data about the communicating branches will be helpful for surgeons who perform surgical procedures in the cervical and axillary regions.


Assuntos
Plexo Braquial/anatomia & histologia , Feminino , Humanos , Masculino , Valores de Referência
12.
Front Cell Infect Microbiol ; 14: 1380209, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38812751

RESUMO

Introduction: The oral cavity and gut tract, being interconnected and rich in microbiota, may have a shared influence on gingivitis. However, the specific role of distinct gut microbiota taxa in gingivitis remains unexplored. Utilizing Mendelian Randomization (MR) as an ideal method for causal inference avoiding reverse causality and potential confounding factors, we conducted a comprehensive two-sample MR study to uncover the potential genetic causal impact of gut microbiota on gingivitis. Methods: Instrumental variables were chosen from single nucleotide polymorphisms (SNPs) strongly associated with 418 gut microbiota taxa, involving 14,306 individuals. Gingivitis, with 4,120 cases and 195,395 controls, served as the outcome. Causal effects were assessed using random-effect inverse variance-weighted, weighted median, and MR-Egger methods. For replication and meta-analysis, gingivitis data from IEU OpenGWAS were employed. Sensitivity analyses included Cochran's Q tests, funnel plots, leave-one-out analyses, and MR-Egger intercept tests. This study aimed to assess the genetic correlation between the genetically predicted gut microbiota and gingivitis using linkage disequilibrium score regression (LDSC). Results: Three gut microbiota taxa (class Actinobacteria id.419, family Defluviitaleaceae id.1924, genus Defluviitaleaceae UCG011 id.11287) are predicted to causally contribute to an increased risk of gingivitis (P< 0.05). Additionally, four gut microbiota taxa (class Actinobacteria id.419, genus Escherichia Shigella id.3504, genus Ruminococcaceae UCG002 id.11360) potentially exhibit inhibitory causal effects on the risk of gingivitis (P< 0.05). No significant evidence of heterogeneity or pleiotropy is detected. Our findings indicate a suggestive genetic correlation between class Actinobacteria id.419, class Bacteroidia id.912, family Defluviitaleaceae id.1924, genus Escherichia Shigella id.3504 and gingivitis. Conclusion: Our study establishes the genetic causal effect of 418 gut microbiota taxa on gingivitis, offering insights for clinical interventions targeting gingivitis. Subsequent research endeavors are essential to corroborate the findings of our present study.


Assuntos
Microbioma Gastrointestinal , Gengivite , Análise da Randomização Mendeliana , Polimorfismo de Nucleotídeo Único , Microbioma Gastrointestinal/genética , Humanos , Gengivite/microbiologia , Desequilíbrio de Ligação , Predisposição Genética para Doença , Actinobacteria/genética , Actinobacteria/isolamento & purificação
13.
Cell Prolif ; : e13699, 2024 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-38943534

RESUMO

Chronic allograft dysfunction (CAD) poses a significant challenge in kidney transplantation, with renal vascular endothelial-to-mesenchymal transition (EndMT) playing a vital role. While renal vascular EndMT has been verified as an important contributing factor to renal allograft interstitial fibrosis/tubular atrophy in CAD patients, its underlying mechanisms remain obscure. Currently, Src activation is closely linked to organ fibrosis development. Single-cell transcriptomic analysis in clinical patients revealed that Src is a potential pivotal mediator in CAD progression. Our findings revealed a significant upregulation of Src which closely associated with EndMT in CAD patients, allogeneic kidney transplanted rats and endothelial cells lines. In vivo, Src inhibition remarkably alleviate EndMT and renal allograft interstitial fibrosis in allogeneic kidney transplanted rats. It also had a similar antifibrotic effect in two endothelial cell lines. Mechanistically, the knockout of Src resulted in an augmented AMBRA1-mediated mitophagy in endothelial cells. We demonstrate that Src knockdown upregulates AMBRA1 level and activates mitophagy by stabilizing Parkin's ubiquitination levels and mitochondrial translocation. Subsequent experiments demonstrated that the knockdown of the Parkin gene inhibited mitophagy in endothelial cells, leading to increased production of Interleukin-6, thereby inducing EndMT. Consequently, our study underscores Src as a critical mediator of renal vascular EndMT and allograft interstitial fibrosis, exerting its impact through the regulation of AMBRA1/Parkin-mediated mitophagy.

14.
Opt Express ; 21(3): 2551-62, 2013 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-23481713

RESUMO

When an optical fiber is dipped in an etching solution, the internal stress profile in the fiber varies with the fiber diameter. We observed a physical contraction as much as 0.2% in the fiber axial dimension when the fiber was reduced from its original diameter to ~6 µm through analysis using high resolution microscope images of the grating period of an etched FBG at different fiber diameters. This axial contraction is related to the varying axial stress profile in the fiber when the fiber diameter is reduced. On top of that, the refractive index of fiber core increases with reducing fiber diameter due to stress-optic effect. The calculated index increment is as much as 1.8 × 10(-3) at the center of fiber core after the diameter is reduced down to ~6 µm. In comparison with the conventional model that assumes constant grating period and neglects the variation in stress-induced index change in fiber core, our proposed model indicates a discrepancy as much as 3nm in Bragg wavelength at a fiber diameter of ~6 µm.


Assuntos
Modelos Teóricos , Fibras Ópticas , Simulação por Computador , Desenho Assistido por Computador , Módulo de Elasticidade , Desenho de Equipamento , Análise de Falha de Equipamento , Estresse Mecânico
15.
Opt Express ; 21(12): 14808-15, 2013 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-23787668

RESUMO

We present a new theoretical model for the broadband reflection spectra of etched FBGs which includes the effects of axial contraction and stress-induced index change. The reflection spectra of the etched FBGs with several different taper profiles are simulated based on the proposed model. In our observation, decaying exponential profile produces a broadband reflection spectrum with good uniformity over the range of 1540-1560 nm. An etched FBG with similar taper profile is fabricated and the experimental result shows good agreement with the theoretical model.


Assuntos
Desenho Assistido por Computador , Tecnologia de Fibra Óptica/instrumentação , Modelos Teóricos , Refratometria/instrumentação , Simulação por Computador , Módulo de Elasticidade , Desenho de Equipamento , Análise de Falha de Equipamento , Luz , Espalhamento de Radiação
16.
Opt Lett ; 38(21): 4409-12, 2013 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-24177106

RESUMO

This Letter presents a simple mathematical model developed from coupled-mode theory to describe the relationship between Bragg transmission loss (BTL), grating length, coupling coefficients, and bending loss in a bent fiber Bragg grating. In our investigation, the finding indicates that the decrement of BTL can be attributed to the increasing bending loss and degradation of both dc and ac coupling coefficients as the bending radius decreases. Besides, the center wavelength shifts as a result of coupling coefficients degradation. The validity of the proposed model is supported by experimental result.

17.
Cell Death Discov ; 9(1): 271, 2023 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-37507403

RESUMO

Chronic allograft dysfunction (CAD) is a major factor that hinders kidney transplant survival in the long run. Epithelial-mesenchymal transition (EMT) has been confirmed to significantly contribute to interstitial fibrosis/tubular atrophy (IF/TA), which is the main histopathological feature of CAD. Aberrant expression of the regulator of calcineurin 1 (RCAN1), recognized as an endogenous inhibitor of the calcineurin phosphatase, has been shown to be extensively involved in various kidney diseases. However, it remains unclear how RCAN1.4 regulates IF/TA formation in CAD patients. Herein, an in vivo mouse renal transplantation model and an in vitro model of human renal tubular epithelial cells (HK-2) treated with tumor necrosis factor-α (TNF-α) were employed. Our results proved that RCAN1.4 expression was decreased in vivo and in vitro, in addition to the up-regulation of Yin Yang 1 (YY1), a transcription factor that has been reported to convey multiple functions in chronic kidney disease (CKD). Knocking in of RCAN1.4 efficiently attenuated chronic renal allograft interstitial fibrosis in vivo and inhibited TNF-α-induced EMT in vitro through regulating anti-oxidative stress and the calcineurin/nuclear factor of activated T cells cytoplasmic 1 (NFATc1) signaling pathway. In addition, suppression of YY1 mediated by shRNA or siRNA alleviated TNF-α-induced EMT through abolishing reactive species partly in an RCAN1.4-dependent manner. Notably, we confirmed that YY1 negatively regulated RCAN1.4 transcription by directly interacting with the RCAN1.4 promoter. In addition, histone deacetylase 2 (HDAC2) interacted with YY1 to form a multi-molecular complex, which was involved in TNF-α-induced RCAN1.4 transcriptional repression. Therefore, RCAN1.4 is suggested to be modulated by the YY1/HDAC2 transcription repressor complex in an epigenetic manner, which is a mediated nephroprotective effect partly through modulating O2⋅- generation and the calcineurin/NFATc1 signaling pathway. Thus, the YY1-RCAN1.4 axis constitutes an innovative target for IF/TA treatment in CAD patients.

18.
Arch Med Sci ; 19(5): 1497-1507, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37732052

RESUMO

Introduction: This study aims to investigate the effects of ivabradine (IVA) on ventricular electrophysiological remodeling after myocardial infarction (MI) in rats. Material and methods: A total of 60 male Sprague-Dawley rats were randomly divided into five groups: an MI group, an IVA group, a metoprolol (MET) group, an IVA + MET group, and a sham group. After a four-week intervention, the ventricular electrophysiological parameters were detected by multichannel electrophysiological polygraph. Then, the morphological characteristics were evaluated using hematoxylin and eosin (H&E) and Masson's staining, and the expression of phosphorylated connexin 43 (p-Cx43) in the left ventricular wall was detected through immunohistochemistry and the Western blot test. Results: The electrophysiological examination revealed that the induction rate and fatality rate of ventricular tachycardia (VT)/ventricular fibrillation (VF) were lower in both the IVA and the MET group, compared with the MI group (6/12, 6/12 vs. 10/11; and 1/12, 1/12 vs. 5/11; all p < 0.05), as well as the IVA + MET group (1/11 vs. 10/11, p < 0.01; and 1/11 vs. 5/11, p < 0.05). The induction rate of VT/VF was lower in the IVA + MET group, compared to the MET group (1/11 vs. 6/12, p < 0.05). H&E and Masson's staining revealed that compared with the MI group, the left ventricular infarction area was lower in the IVA, MET, and IVA + MET groups (p < 0.05, p < 0.05, and p < 0.01, respectively), while collagen volume fraction (CVF) also was lower in the other groups (all p < 0.01). The left ventricular infarction area and CVF both were lower in the IVA + MET group, compared to the MET group (p < 0.05 and p < 0.01, respectively). The immunohistochemistry and Western blot revealed that p-Cx43 expression was higher in the treatment groups, compared with the MI group (all p < 0.01). Conclusions: IVA can reduce the incidence of ventricular arrhythmia after MI in male rats by improving both structural and electrical remodeling, and the combination of IVA and MET is even more effective.

19.
Huan Jing Ke Xue ; 44(11): 6137-6148, 2023 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-37973097

RESUMO

To investigate the distribution characteristics of the cyanobacteria community and the driving factors in impounded lakes and reservoirs in Shandong on the east route of the South-to-North Water Diversion Project, monthly samples of phytoplankton and the aquatic environment from Nansi Lake, Dongping Lake, Datun Reservoir, Donghu Reservoir, and Shuangwangcheng Reservoir were collected from May to November during 2010 to 2019. A total of 44 planktonic cyanobacteria taxa were identified with 23 filamentous cyanobacteria taxa. Pseudanabaena limnetica, Cylindrospermopsis raciborskii, Microcystis aeruginosa, and Microcystis wesenbergii were the dominant harmful cyanobacteria species, with a high detection frequency and abundance in all lakes and reservoirs. By analyzing the distribution characteristics of the cyanobacteria community in impounded lakes and reservoirs, we found that filamentous cyanobacteria had growth advantages in the water with large hydraulic disturbances, which should be the key points of cyanobacteria prevention and control in the future. Pearson correlation analysis and generalized linear fitting curve results showed that total nitrogen, total phosphorus, water temperature, and water depth played a key role in affecting the growth of P. limnetica, C. raciborskii, M. aeruginosa, and M. wesenbergii. The nitrogen and phosphorus nutrients could promote the growth of harmful cyanobacteria. Due to the good temperature adaptability, P. limnetica could still become the dominant species in early summer and late autumn, and C. raciborskii, M. aeruginosa, and M. wesenbergii had growth advantages when the water temperature was higher than 25℃. In addition, shallow water was more conducive to the growth of C. raciborskii. It was suggested that based on strengthening of the control of nitrogen and phosphorus nutrient input in lakes and reservoirs, the key monitoring of P. limnetica in lakes should be conducted in early summer and late autumn, and the growth of C. raciborskii in shallow water areas should be paid close attention in the high temperature period to ensure the safety of water quality.


Assuntos
Cianobactérias , Lagos , Lagos/microbiologia , Monitoramento Ambiental , Fitoplâncton , Fósforo/análise , Nitrogênio/análise
20.
Chemosphere ; 307(Pt 1): 135642, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35817186

RESUMO

Iron (Fe) plaque is an important component of rice roots because it influences the uptake and transport of cadmium (Cd) in rice. In this study, a hydroponic experiment was developed to investigate the influence of phosphorus (P) on the formation of iron plaque on the root surface as well as the influence on Cd uptake in rice plants. Three important results were obtained, as follows. (1) During the formation of iron plaque induced by exogenous Fe, P supply was beneficial for the iron plaque formation, but it restrained the Cd retention capability, resulting in a decrease in Cd in iron plaque by 35.48-61.93%, and leading to an increase in Cd in rice roots from 72.13 mg kg-1 to 112.78 mg kg-1 (2) After the iron plaque induction, the formation of iron plaque was inhibited by P supply, resulting in the amount of iron plaque decreasing by 18.46-54.57%, and the Cd in iron plaque decreased by 3.93-31.78%. Then, the Cd retention capability and the prevention effect simultaneously decreased, and as a result, the Cd in rice roots increased from 100.83 mg kg-1 to 146.03 mg kg-1 (3) Without exogenous Fe induction, P sufficiency continued to increase the amount of iron plaque and decrease the Cd in iron plaque, and increase the Cd in rice plants. These results suggested that P supply increases the amount of iron plaque, which is ineffective for Cd retention, such as non-reddish-brown iron plaque (NIP), and then decreases the capacity of iron plaque to retain Cd. Additionally, the P supply decreased the amount of formed iron plaque, causing the decreasing prevention effect. Therefore, excessive application of P fertilizer should be avoided in Cd-contaminated paddy fields.


Assuntos
Oryza , Poluentes do Solo , Cádmio/análise , Fertilizantes/análise , Ferro/análise , Fósforo/farmacologia , Raízes de Plantas , Solo , Poluentes do Solo/análise
SELEÇÃO DE REFERÊNCIAS
Detalhe da pesquisa