RESUMO
Murine embryonal carcinoma F9 cells, a tissue culture model for early embryonic development, do not produce interferon (IFN) in response to poly(I-C), as determined by an antiviral assay. RNase protection analyses were used to examine total RNA extracted from the cells for the presence of beta-IFN RNA. Whereas F9 cells differentiated in vitro with retinoic acid produced a biologically active protein as well as beta-IFN RNA in response to poly(I-C), undifferentiated F9 cells produced no detectable beta-IFN RNA even in the presence of cycloheximide, an IFN-superinducing agent. These results show that undifferentiated embryonal carcinoma cells do not accumulate beta-IFN RNA in response to an IFN-inducing agent, suggesting a transcriptional regulatory mechanism. However, this control mechanism is altered upon differentiation, since the gene can be transcriptionally activated in retinoic acid-differentiated cells.
Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Interferon Tipo I/genética , Células-Tronco Neoplásicas/metabolismo , Animais , Diferenciação Celular/efeitos dos fármacos , Cicloeximida/farmacologia , Células-Tronco de Carcinoma Embrionário , Camundongos , Poli I-C/farmacologia , RNA Mensageiro/metabolismo , Ribonucleases , Tretinoína/farmacologia , Células Tumorais CultivadasRESUMO
Five clones of Chinese hamster cells transformed with simian virus 40 (SV40) were isolated from methylcellulose and characterized as to Giemsa-banded karyotype, DNA content, saturation density, agglutination with concanavalin A, and tumorigenicity. Chromosome analysis and DNA content studies at early passage revealed that the genetic complement for all clones was predominantly near tetraploid. All cultures examined contained a proportion of hypertetraploid cells. Nonrandom chromosome changes included at least one broken No 1 chromosone in 80% or more of the cells in each clone, and fewer sex chromosomes than anticipated from the ploidy of the cells. Several abnormal marker chromosomes tended to recur. These changes were more pronounced in the cells cultured from tumors formed by three of the clones. A karyotypically stable stem line was not noted for any of the clones or tumors. The functional significance of the karyotypic heterogeneity was assessed by means of cloning efficiencies both on plastic and in methylcellulose.
Assuntos
Transformação Celular Neoplásica , Aberrações Cromossômicas , Vírus 40 dos Símios , Animais , Antígenos Virais , Divisão Celular , Células Clonais/ultraestrutura , Cricetinae , DNA de Neoplasias/análise , Cariotipagem , Transplante de Neoplasias , Neoplasias Experimentais/análise , Neoplasias Experimentais/genética , Neoplasias Experimentais/imunologia , Ploidias , Vírus 40 dos Símios/imunologia , Transplante HomólogoRESUMO
A clone of Chinese hamster embryo cells transformed by tsA58, the temperature-sensitive mutant of simian virus 40, was analyzed for chromosome abnormalities at the permissive temperature (37 degrees C) and nonpermissive temperature (40.5 degrees C). Trypsin-Giemsa-banded metaphases were analyzed 1 week after the temperature shift. The metaphases from cells at both temperatures were pseudodiploid, with numerous chromosome changes primarily affecting chromosomes no. 1 and 2. Other chromosomes (no. 6, 11, and the X) were also frequently involved. A marker chromosome, LM, was present in 35% of the cells at 40.5 degrees C.
Assuntos
Transformação Celular Neoplásica , Aberrações Cromossômicas , Mutação , Vírus 40 dos Símios/genética , Temperatura , Animais , Células Clonais/patologia , CricetinaeRESUMO
Chinese hamster embryo cells transformed with the tRNA catabolite 1-methylguanine were characterized by Giemsa-banded karyotyping and by their tumorigenic potency in athymic nude mice. All seven 1-methylguanine-transformed cell lines were hyperdiploid with a modal chromosome number of 23. Three of these lines had an additional marker chromosome derived from the long (q) arm of chromosome no. 4, and they had alterations of chromosome no. 5 as well. Two of these three cell lines were tumorigenic. Nonrandom chromosome changes were observed in the other four 1-methylguanine-transformed cell lines, which included the addition of all or a portion of chromosome no. 6. One of these cell lines was also tumorigenic in nude mice, Specific cytogenetic changes were observed in most 1-methylguanine-transformed populations in contrast to the karyotypic heterogeneity of a benzo[a]pyrene-transformed cell line.
Assuntos
Transformação Celular Neoplásica , Aberrações Cromossômicas , Guanina/análogos & derivados , Neoplasias Experimentais/genética , Animais , Benzopirenos , Linhagem Celular , Cricetinae , Cricetulus , Embrião de Mamíferos , Camundongos , Camundongos Nus , Transplante de Neoplasias , Neoplasias Experimentais/induzido quimicamente , Transplante HeterólogoRESUMO
Doxorubicin does not kill L929 cells at concentrations that profoundly reduce clonogenic survival. Instead, the cell and nuclear volume progressively increase for at least 1 week following drug exposure leading to the production of characteristic giant cells. The increase in nuclear volume is due to continued DNA synthesis and increase in chromosome number without entry into mitosis. The implications of this finding for in vitro chemosensitivity assays and for the mechanism of doxorubicin cytotoxicity are discussed.
Assuntos
Replicação do DNA/efeitos dos fármacos , DNA/efeitos dos fármacos , Doxorrubicina/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , DNA/biossíntese , Citometria de Fluxo , Cariotipagem , Cinética , Células L/citologia , Células L/efeitos dos fármacos , CamundongosRESUMO
The lactate dehydrogenase (LDH) isozyme patterns of consecutive passages of Chinese hamster embryo cultures were monitored. At early passages the population displayed two LDH bands, M4 and M3H; however, at higher passages the cultures exhibited M2H2, M2H, and M4. When primary cultures of Chinese hamster embryo cells were infected with simian virus 40 (SV40), no change in the LDH pattern was observed; however, the total activity of LDH increased. Twenty-three of 25 transformed colonies isolated from SV40-infected primary cells by their ability to grow in methyl cellulose produced only M4 or M4-M3H isozymes bands. Four of the SV40-transformed clones that produced only the M4 isozyme were tested for LDH activity and found to have activities 2.5 to 3 times greater than the control cells. Chinese hamster kidney epithelial cells transformed with SV40 virus had a decrease in the H subunit production, from 57 to 31%, compared with normal kidney epithelial cells. This decrease in H subunit production led to an increase in the cathode-migrating isozymes. Therefore, a shift to the cathode-migrating isozyme was observed in SV40-transformed cells. This change in LDH pattern might represent a reversion to the enzyme pattern present in fetal cells.
Assuntos
Transformação Celular Neoplásica , L-Lactato Desidrogenase/metabolismo , Neoplasias Experimentais/enzimologia , Vírus 40 dos Símios , Animais , Células Cultivadas , Cricetinae , Eletroforese em Gel de Ágar , Embrião de Mamíferos , Isoenzimas , Rim , Fatores de TempoRESUMO
A series of human germ cell-derived tumors were examined for the presence of nucleoli which persist through mitosis. Embryonal carcinomas, seminomas, and the cytotrophoblasts of choriocarcinomas had persistent nucleoli in more than 70% of mitoses. The differentiated cells derived from embryonal carcinomas, endodermal sinus tumors, and the more differentiated elements of choriocarcinomas only rarely had persistent nucleoli. These nucleoli appeared to remain transcriptionally active.
Assuntos
Núcleo Celular/ultraestrutura , Disgerminoma/ultraestrutura , Neoplasias Testiculares/ultraestrutura , Coriocarcinoma/ultraestrutura , Humanos , Masculino , Mitose , Coloração e Rotulagem , Teratoma/ultraestruturaRESUMO
The multipotential stem cell of the murine tetratocarcinoma, embryonal carcinoma (EC), is capable of differentiation in vivo and in vitro to nonneoplastic progeny. Undifferentiated EC cells, spontaneously differentiating tetratocarcinoma cells, and differentiated cells derived from EC cells were analyzed for DNA content and chromosome number distributions. Flow microfluorometric and fluorescence cytophotometric analysis of DNA content showed that EC cells had a characteristic diploid (2c) distribution, whereas several differentiated cell lines derived from EC cells had 4c DNA distributions. The tetraploid cell populations studied were capable of cell division but had restricted differentiative potential and were either of low tumorigenicity or non-tumorigenic. In vivo teratocarcinomas, comprised of both EC cells and differentiated cell types, contained diploid and tetraploid populations. Chromosomally, EC cells were neardiploid (39 chromosomes) and differentiated cells were near-tetraploid (62 to 76 chromosomes). The teratocarcinoma provides a model for studying the basic mechanisms that control the growth dynamics of the rapidly and slowly proliferating cell populations present in many tumors.
Assuntos
DNA de Neoplasias/análise , Teratoma/análise , Neoplasias Testiculares/análise , Diferenciação Celular , Divisão Celular , Células Cultivadas , DNA/análise , Diploide , Fluorometria , Masculino , Neoplasias Experimentais/análise , PoliploidiaRESUMO
A detailed morphological analysis of Walker 256 cells sensitive and resistant to cis-diamminedichloroplatinum(II) has been performed. Two cell populations are identified by electron microscopy of differing differentiation corresponding structurally to cells reported in experimentally induced metastases. Phenotyping of the cells using a number of monoclonal antibodies by immunocytochemistry and flow cytometry showed the absence of epithelial cell markers: however, the cells stained intensely for markers for germ and/or hematopoietic cells. Further studies utilizing monoclonal antibodies to lymphoid, myeloid, and monocytoid cells showed the cells to be monocytoid in origin. No evidence of cell heterogeneity was evident from the phenotypic experiments (a biphasic pattern was not observed). Enzyme histochemistry showed strong focal acid phosphatase activity suggestive of cells of hematopoietic origin. Thus the concept that these cells reflect an epithelial cell of origin is not substantiated by phenotyping with two methodologies.
Assuntos
Carcinoma 256 de Walker/patologia , Animais , Linhagem Celular , Cisplatino/farmacologia , DNA/análise , Resistência a Medicamentos/genética , Citometria de Fluxo , Imuno-Histoquímica , Microscopia Eletrônica , Fenótipo , RatosRESUMO
Infection of normal human diploid fibroblasts (HF) with the DNA tumor virus simian virus 40 (SV) leads to an extension of lifespan and concomitant increase in the levels of the viral large tumor antigen (T antigen) and the cellular protein p53. The intracellular localization of T antigen and p53 was mostly nuclear in both SVpre-crisis and SVpost-crisis cells, however certain population doubling (PD) of the SVpre-crisis cells exhibited some cytoplasmic staining. The DNA content of SVpre-crisis cells shifted to tetraploidy and the SVpost-crisis cells were near-tetraploid. Quantitation of T antigen and p53 in single cells by flow cytometry demonstrated that for all antibodies tested the levels of T antigen were higher in the SVpre-crisis HF than in the SVpost-crisis. The quantity of p53 increased with increasing age of SVpre-crisis HF, and the levels of p53 were higher in the SVpost-crisis HF populations. Immunoprecipitation of p53, T antigen and complexes demonstrated that all p53 was bound to T antigen in SVpre-crisis HF and SVpost-crisis HF. The SVpre-crisis HF cells showed that 33% of all T antigen was bound to p53, while 67% was free, and the SVpost-crisis HF exhibited 50% free T antigen and 50% bound to p53. The half-life of p53 was similar in all SVpre-crisis HF; however, the half-life was 2-3 times greater in SVpost-crisis HF than in SVpre-crisis HF. These results suggest that the interaction of DNA (ploidy), T antigen, p53 and complexes may be involved in formation of a stable SV40-transformed human cell line.
Assuntos
Antígenos Transformantes de Poliomavirus/genética , Vírus 40 dos Símios/genética , Proteína Supressora de Tumor p53/genética , Anticorpos Monoclonais , Antígenos Transformantes de Poliomavirus/análise , Divisão Celular , Linhagem Celular Transformada , DNA/análise , Citometria de Fluxo , Humanos , Proteína Supressora de Tumor p53/análiseRESUMO
Simian virus 40 (SV40) infection of monkey kidney cells induces successive rounds of cellular DNA synthesis without intervening mitosis. To gain an understanding of the mechanisms responsible for disruption of cell cycle control during lytic infection, pRB phosphorylation and cell cycle distribution were examined following SV40 infection of CV-1 cells. The hypophosphorylated pRB present in confluent CV-1 cells was phosphorylated within 14 h following SV40 infection. Phosphorylated pRB then remained the predominant form as cells progressed from late G1 through S phase. Hypophosphorylated pRB reappeared as cells moved through G2 and acquired a tetraploid (> G2) DNA content. The reappearance of hypophosphorylated pRB in a population with decreasing numbers of cells in G1 phase and increasing numbers of cells in > G2 suggests that accumulation of hypophosphorylated pRB may be involved in T antigen-induced tetraploidy.
Assuntos
Ciclo Celular , Transformação Celular Neoplásica , DNA/metabolismo , Poliploidia , Proteína do Retinoblastoma/metabolismo , Vírus 40 dos Símios/genética , Animais , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , DNA/genética , Fase G1 , Cinética , Mimosina/farmacologia , Fosforilação , Proteína do Retinoblastoma/biossíntese , Fase SRESUMO
We have compared the structure and composition of adult and fetal bovine bone marrow extracellular matrices. In contrast to fetal bone marrow, adult bone marrow has more oval fenestration and accumulation of adipocytes as well as lower protein content. These differences could be due to remodeling of bone marrow tissue as it develops. Zymogram analysis of matrix metalloproteinase (MMP) and tissue inhibitor of MMP (TIMP) activities showed that fetal, but not adult bone marrow extract contained a 96-kDa MMP and TIMP-1 and -2. These activities may contribute to the structural differences between adult and fetal bone marrow tissues.
Assuntos
Medula Óssea/crescimento & desenvolvimento , Matriz Extracelular/ultraestrutura , Fatores Etários , Animais , Medula Óssea/metabolismo , Medula Óssea/ultraestrutura , Bovinos , Eletroforese em Gel de Poliacrilamida , Desenvolvimento Embrionário e Fetal , Matriz Extracelular/metabolismo , Secções Congeladas , Metaloendopeptidases/análise , Microscopia Eletrônica de Varredura , Extratos de Tecidos , Inibidores Teciduais de Metaloproteinases/análiseRESUMO
Wild type polyomavirus expression is suppressed in embryonal carcinoma (EC) cell lines. This suppression is alleviated when the EC cells are induced to differentiate. Several characterized host range mutants of polyoma overcome suppression and are able to express and replicate in the undifferentiated EC cells. These previously described isolates were obtained by serial passage of a wild type strain through PCC4 or F9 EC lines. We present a new pyPCC4 isolate (LPT) derived without selection in EC cells. Isolates with host range specificity for a given EC line have been reported to share several common rearrangements and features. These features are also observed in LPT. We report a novel feature shared by these mutants, including LPT, capable of expression in the EC cell line PCC4. In 8 of 10 isolates a novel sequence is created within the enhancer region by rearrangement junctions with near perfect homology to the AP-1 core consensus sequence, 'TGACT(C/A)A'. That the precise location of these junctions varies among these isolates suggest a functional role for this conserved sequence. Our goal is to understand the function of various mutations in host range mutants of polyoma. In order to understand the rearrangements necessary for expression and replication of polyoma in PCC4 cells, we have further characterized the limits of the B enhancer in these cells as compared to those described in permissive cell systems. We have been able to locate the origin proximal limit of the B enhancer for replication close to nt 5189 and distinguish it from the origin proximal limit of the B enhancer for transcription near nt 5215. The two B enhancer cores overlap but do not coincide and are conserved in both cell lines.
Assuntos
Regulação Neoplásica da Expressão Gênica , Células-Tronco Neoplásicas , Polyomavirus/genética , Teratoma/genética , Animais , Sequência de Bases , Sequência Consenso , Células-Tronco de Carcinoma Embrionário , Elementos Facilitadores Genéticos , Camundongos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Teratoma/microbiologia , TransativadoresRESUMO
We have explored the possibility that estrogens and progesterone could have different target uterine cell populations according to their cell cycle stage and localization in glands vs. lumen. Experiments were carried out in which rabbits were injected with [3H]thymidine for 3 days to label nuclei of dividing cells, then either 17 beta-estradiol, progesterone, or vehicle were administered. 17 beta-Estradiol induced a decrease in the percentage of cells with labeled nuclei or labeling index of either luminal or glandular epithelium. Since this steroid has been shown to have a significant proliferative effect on glands, the data suggest that its effect is exerted on unlabeled quiescent cells, which are then recruited into the cell cycle. Progesterone, on the other hand, was found to induce a significant increase in labeling index of both luminal and glandular epithelium. Therefore, it is concluded that dividing cells are a target for this hormone. Analysis of the number of nuclear grains according to cell location in luminal vs. glandular epithelia and the effect of hormone administration confirmed that each ovarian hormone acts on different target cell populations. Short and long term administration of estrogens resulted in a larger internal circumference of the uterus due to an increase in the number of luminal cells, whereas the number of glands and glandular cells per section did not appear to change. These findings, in combination with previous research, suggest that endometrial gland cells migrate towards the lumen and estrogen administration decreases the rate of cell loss in the luminal epithelium. The concept of cell migration is supported by experiments in which single administration of [3H]thymidine to rabbits was followed by determination at different times of the geographical distribution of cells with labeled nuclei. There was observed, as a function of time, a decrease in the number of labeled cells in the bottom of the glands with a concomitant increase in the same parameter in the upper part of the glands and luminal epithelia. Estradiol administration changed these kinetics.
Assuntos
Estradiol/farmacologia , Progesterona/farmacologia , Útero/fisiologia , Animais , Castração , Ciclo Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Replicação do DNA/efeitos dos fármacos , Epitélio/efeitos dos fármacos , Epitélio/fisiologia , Estradiol/análogos & derivados , Congêneres do Estradiol/farmacologia , Feminino , Cinética , Coelhos , Útero/efeitos dos fármacosRESUMO
Cells transformed by the simian virus 40 (SV40) large T antigen (Tag) contain elevated levels of cellular p53 protein. To quantify this relationship, levels of p53 were measured in NIH 3T3 cells that expressed different concentrations of Tag. Using immunoblotting, average p53 levels were shown to increase linearly with Tag concentrations in these cell lines. Single-cell measurements were also performed using flow cytometry to measure p53 immunofluorescence. Surprisingly, the flow cytometry experiments showed that two distinct cell populations, based on p53 content, were present in cells expressing high levels of Tag. One cell population contained elevated p53 levels. A second population did not contain elevated p53, even though high concentrations of Tag were present in the cells. This latter cell population did not appear to arise because of mutations in either Tag or p53. The two cell populations also had phenotypic differences. In exponentially growing cells, Tag alters the cell cycle distribution (decreases the percentage of G1 phase cells and increases the percentages of S and G2 + M phase cells). This phenotype was maximum in the cell population containing elevated p53. A lesser phenotype was found in the cell population that did not contain elevated p53. These data show, firstly, that cells can express significant levels of Tag and not contain elevated levels of p53 and, secondly, that elevated p53 correlates with the altered cell cycle distribution produced by Tag in growing cells.
Assuntos
Antígenos Transformantes de Poliomavirus/análise , Ciclo Celular/fisiologia , Proteína Supressora de Tumor p53/análise , Células 3T3 , Animais , Antígenos Transformantes de Poliomavirus/genética , Linhagem Celular Transformada/química , Linhagem Celular Transformada/citologia , Linhagem Celular Transformada/virologia , DNA/análise , Citometria de Fluxo , Imunofluorescência , Regulação Viral da Expressão Gênica , Immunoblotting , Imunofenotipagem , Camundongos , Proteína Supressora de Tumor p53/genéticaRESUMO
Proteoliposome delivery vesicles can be prepared by the protein-cochleate method [Gould-Fogerite and Mannino, Anal. Biochem. 148 (1985) 15-25; Mannino and Gould-Fogerite, Biotechniques 6 (1988) 682-690]. Proteins which mediate the entry of enveloped viruses into cells are integrated in the lipid bilayer, and materials are encapsulated at high efficiency within the aqueous interior of these vesicles. We describe proteoliposome-mediated delivery of proteins and drugs into entire populations of cells in culture. Material can be delivered gradually by Sendai-virus-glycoprotein-containing proteoliposomes. Alternatively, synchronous delivery to a population can be achieved by exposing cell-bound influenza glycoprotein vesicles briefly to low pH buffer. When DNA is encapsulated, chimeric proteoliposome gene-transfer vesicles (chimerasomes), which mediate high-efficiency gene transfer in vitro and in vivo, are produced. Stable expression of a bovine papilloma virus-based plasmid in tissue-cultured cells, at 100,000 times greater efficiency than Ca.phosphate precipitation of DNA, with respect to the quantity of DNA used, has been achieved. Stable gene transfer and expression in mice has been obtained by subcutaneous injection of chimerasomes containing a plasmid expressing the early region of polyoma virus. In one experimental group, 50% of the mice developed tumors which were shown to express polyoma virus early proteins and contain the transferred DNA. This is the first report of stable gene transfer in animals mediated by a liposome- or proteoliposome-based system.
Assuntos
Proteolipídeos , Transfecção , Proteínas do Envelope Viral/genética , Animais , Anticorpos/administração & dosagem , DNA Viral/genética , Portadores de Fármacos , Técnicas Genéticas , Glicoproteínas , Concentração de Íons de Hidrogênio , Isoquinolinas/imunologia , Camundongos , Camundongos Endogâmicos AKR , Orthomyxoviridae/genética , Vírus da Parainfluenza 1 Humana/genética , Plasmídeos/genética , Células Tumorais Cultivadas , Proteínas Virais/biossíntese , Proteínas Virais/genéticaRESUMO
Much work on psychosocial sequelae of breast cancer has been guided by the assumption that body image and partner reaction issues are focal. In a tri-ethnic sample of 223 women treated for early-stage breast cancer within the prior year, the authors assessed a wider range of concerns and relations to well-being. Strongest concerns were recurrence, pain, death, harm from adjuvant treatment, and bills. Body-image concerns were moderate; concern about rejection was minimal. Younger women had stronger sexual and partner-related concerns than older women. Hispanic women had many stronger concerns and more disruption than other women. Life and pain concerns and sexuality concerns contributed uniquely to predicting emotional and psychosexual disruption; life and pain concerns and rejection concerns contributed to predicting social disruption. In sum, adaptation to breast cancer is a process bearing on several aspects of the patient's life space.
Assuntos
Adaptação Psicológica , Neoplasias da Mama/psicologia , Etnicidade/psicologia , Papel do Doente , Ajustamento Social , Adulto , Idoso , Idoso de 80 Anos ou mais , Ansiedade/psicologia , Imagem Corporal , Neoplasias da Mama/patologia , Comparação Transcultural , Feminino , Identidade de Gênero , Humanos , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Qualidade de VidaRESUMO
The authors tested effects of a 10-week group cognitive-behavioral stress management intervention among 100 women newly treated for Stage 0-II breast cancer. The intervention reduced prevalence of moderate depression (which remained relatively stable in the control condition) but did not affect other measures of emotional distress. The intervention also increased participants' reports that having breast cancer had made positive contributions to their lives, and it increased generalized optimism. Both remained significantly elevated at a 3-month follow-up of the intervention. Further analysis revealed that the intervention had its greatest impact on these 2 variables among women who were lowest in optimism at baseline. Discussion centers on the importance of examining positive responses to traumatic events--growth, appreciation of life, shift in priorities, and positive affect-as well as negative responses.
Assuntos
Terapia Comportamental , Neoplasias da Mama/psicologia , Terapia Cognitivo-Comportamental , Transtorno Depressivo/prevenção & controle , Adulto , Idoso , Neoplasias da Mama/complicações , Neoplasias da Mama/terapia , Transtorno Depressivo/epidemiologia , Feminino , Humanos , Acontecimentos que Mudam a Vida , Pessoa de Meia-Idade , Prevalência , Estresse PsicológicoRESUMO
This study explored factors other than medical condition and treatments which contributed to the discharge experiences of 12 rural and 9 urban patients. Interpretive research methodology included document review, observation and in-depth interviews of all key participants. The purposefully selected sample consisted of a total of 21 patients, 22 informal caregivers, and 117 professionals involved in the hospital and/or home setting. Findings document a new perspective on how patients and professionals together contribute to the patient's threatened autonomy. Lack of clarity about goals, aspirations, and purpose in life and a generally negative frame of mind in the elderly combine with professional practice approaches to create a disempowering process. Faced with the biomedical orientation and paternalism of professionals, patients with a positive mindset and sense of direction and purpose in life did not experience threat to their autonomy. The researchers conclude that empowerment strategies must encompass a patient-centred approach, which includes an understanding of the patient's mindset, goals, aspirations, and sense of purpose within a larger life context. This consideration is essential to enable elderly patients to maintain autonomy despite continued health care requirements.
Assuntos
Idoso , Liberdade , Participação do Paciente , Autonomia Pessoal , Relações Médico-Paciente , Idoso de 80 Anos ou mais , Feminino , Serviços de Assistência Domiciliar , Humanos , Masculino , PaternalismoRESUMO
The changes in cell size and total protein were determined for G1-arrested, contact-inhibited CV-1 cells infected with Simian virus 40 (SV40). The assays used were the Biorad total protein assays (Bradford and DC protein assays) on a standard number of cells, total protein as assayed by fluorescein isothiocyanate (FITC) and SR101 by flow cytometry, orthoganol (90 degrees) light scatter by flow cytometry, and direct microscopic measurement with an ocular micrometer. Uninfected CV-1 cells and two cell lines with variations in DNA content (diploid vs. tetraploid) were used as controls for the studies presented. The results demonstrated a 40-60% increase in total protein at 32 to 42 h postinfection. These increases were similar to values obtained due to cellular changes resulting from viral replication and cell death.