CAIDE dementia risk score relates to severity and progression of cerebral small vessel disease in healthy midlife adults: the PREVENT-Dementia study.

BACKGROUND: Markers of cerebrovascular disease are common in dementia, and may be present before dementia onset. However, their clinical relevance in midlife adults at risk of future dementia remains unclear. We investigated whether the Cardiovascular Risk Factors, Ageing and Dementia (CAIDE) risk score was associated with markers of cerebral small vessel disease (SVD), and if it predicted future progression of SVD. We also determined its relationship to systemic inflammation, which has been additionally implicated in dementia and SVD. METHODS: Cognitively healthy midlife participants were assessed at baseline (n=185) and 2-year follow-up (n=158). To assess SVD, we quantified white matter hyperintensities (WMH), enlarged perivascular spaces (EPVS), microbleeds and lacunes. We derived composite scores of SVD burden, and subtypes of hypertensive arteriopathy and cerebral amyloid angiopathy. Inflammation was quantified using serum C-reactive protein (CRP) and fibrinogen. RESULTS: At baseline, higher CAIDE scores were associated with all markers of SVD and inflammation. Longitudinally, CAIDE scores predicted greater total (p<0.001), periventricular (p<0.001) and deep (p=0.012) WMH progression, and increased CRP (p=0.017). Assessment of individual CAIDE components suggested that markers were driven by different risk factors (WMH/EPVS: age/hypertension, lacunes/deep microbleeds: hypertension/obesity). Interaction analyses demonstrated that higher CAIDE scores amplified the effect of age on SVD, and the effect of WMH on poorer memory. CONCLUSION: Higher CAIDE scores, indicating greater risk of dementia, predicts future progression of both WMH and systemic inflammation. Findings highlight the CAIDE score's potential as both a prognostic and predictive marker in the context of cerebrovascular disease, identifying at-risk individuals who might benefit most from managing modifiable risk.

Colorimetry as a tool for description of some wood species marketed as "tauari" in Brazilian Amazon.

The aim of this study was to verify the potential of the colorimetric technique in the identification of some species marketed as "tauari" in the Brazilian Amazon. CIE L* a* b* parameters were applied to determine the colour of 35 wood samples from the main wood poles of the State of Para, Brazil, and the scientific collections of the Museu Paraense Emilio Goeldi (Walter A. Egler Collection) and Embrapa Amazônia Oriental. From each sample, data were obtained in the three wood surfaces: transversal, longitudinal tangential and longitudinal radial. The coordinate b*, which showed the yellow pigment, exerted greater weight in the color characterization of the 35 samples marketed as tauari, being more evident in the tangential and radial sections. In PCA, MGW wood samples showed considerably distinct color patterns in relation to PA and IAN samples, and the h and L* parameters provided better informations for distinguishing species from sample sources. The colorimetric technique can be used as an auxiliary tool in the identification of wood. However, the simultaneous use of colorimetry with the anatomical description of wood is suggested, given the complexity of the species-level separation in "tauari" group.

Proximity to dementia onset and multi-modal neuroimaging changes: The prevent-dementia study.

BACKGROUND: First-degree relatives of people with dementia (FH+) are at increased risk of developing Alzheimer's disease (AD). Here, we investigate "estimated years to onset of dementia" (EYO) as a surrogate marker of preclinical disease progression and assess its associations with multi-modal neuroimaging biomarkers. METHODS: 89 FH+ participants in the PREVENT-Dementia study underwent longitudinal MR imaging over 2 years. EYO was calculated as the difference between the parental age of dementia diagnosis and the current age of the participant (mean EYO = 23.9 years). MPRAGE, ASL and DWI data were processed using Freesurfer, FSL-BASIL and DTI-TK. White matter lesion maps were segmented from FLAIR scans. The SPM Sandwich Estimator Toolbox was used to test for the main effects of EYO and interactions between EYO, Time, and APOE-ε4+. Threshold free cluster enhancement and family wise error rate correction (TFCE FWER) was performed on voxelwise statistical maps. RESULTS: There were no significant effects of EYO on regional grey matter atrophy or white matter hyperintensities. However, a shorter EYO was associated with lower white matter Fractional Anisotropy and elevated Mean/Radial Diffusivity, particularly in the corpus callosum (TFCEFWERp < 0.05). The influence of EYO on white matter deficits were significantly stronger compared to that of normal ageing. APOE-ε4 carriers exhibited hyperperfusion with nearer proximity to estimated onset in temporo-parietal regions. There were no interactions between EYO and time, suggesting that EYO was not associated with accelerated imaging changes in this sample. CONCLUSIONS: Amongst cognitively normal midlife adults with a family history of dementia, a shorter hypothetical proximity to dementia onset may be associated with incipient brain abnormalities, characterised by white matter disruptions and perfusion abnormalities, particularly amongst APOE-ε4 carriers. Our findings also confer biological validity to the construct of EYO as a potential stage marker of preclinical progression in the context of sporadic dementia. Further clinical follow-up of our longitudinal sample would provide critical validation of these findings.

A contribution to the identification of charcoal origin in Brazil II - Macroscopic characterization of Cerrado species.

The Brazilian Cerrado is the richest savanna in the world. It is also one of the biomes more threatened in the country and a hotspot for conservation priorities. The main causes of deforestation in Cerrado are agricultural practices, livestock and charcoal production. Although charcoal has a minor impact, its consumption represents the deforestation of 16.000 Km² of the Cerrado. To contribute for the biomes's conservation it is very important to improve forestry supervision. Thus, in this work we present the macroscopic characterization of charcoal from 25 Cerrado's species. We simulate the real conditions of forest controllers by using the magnifications of 10x, 25x and 65x. Likewise, the charcoals micrographs are all of transverse sections due to the larger amount of anatomical information. We also analyzed texture, brightness, vitrification, ruptures and some special features. The species present several differences in their anatomical structure. Although some of them are very unique, this work does not intent to identify charcoals only by macroscopic analyses. But it might give directions to future identification of genera or species. It also provides knowledge for government agents to verify the documents of forestry origin by fast analyzing a sample of charcoal itself.

Assessment of Nano Cellulose from Peach Palm Residue as Potential Food Additive: Part II: Preliminary Studies.

High consumption of dietary fibers in the diet is related to the reduction of the risk of non-transmitting of chronic diseases, prevention of the constipation etc. Rich diets in dietary fibers promote beneficial effects for the metabolism. Considering the above and recognizing the multifaceted advantages of nano materials, there have been many attempts in recent times to use the nano materials in the food sector including as food additive. However, whenever new product for human and animal consumption is developed, it has to be tested for their effectiveness regarding improvement in the health of consumers, safety aspects and side effects. However, before it is tried with human beings, normally such materials would be assessed through biological tests on a living organism to understand its effect on health condition of the consumer. Accordingly, based on the authors' finding reported in a previous paper, this paper presents body weight, biochemical (glucose, cholesterol and lipid profile in blood, analysis of feces) and histological tests carried out with biomass based cellulose nano fibrils prepared by the authors for its possible use as food additive. Preliminary results of the study with mice have clearly brought out potential of these fibers for the said purpose.

Valorization of feather waste in Brazil: structure, methods of extraction, and applications of feather keratin.

This systematic review presents the potential of using feather waste as a ß-keratin source, including the Brazilian scenario in the generation of this byproduct. The structure and properties of α- and ß-keratin, the methods commonly reported to extract keratin from poultry feathers, and applications of feather keratin-based materials are also covered in this review. The literature search for poultry production data in Brazil was conducted for the last 2 years, for the period 2021-2022. A broad literature search for extraction methods and applications of feather keratin was done for the period 2001-2022. The poultry industry is one of the largest sectors of the food industry, and Brazil was the third-largest world producer of chicken meat with more than six billion chickens slaughtered in 2021. Poultry feathers constitute about 7% weight of broilers; thus, it can be estimated that about one million tons of poultry feathers were generated in Brazil in 2021, and the improper disposal of this byproduct contributes to environmental problems and disease transmission. The most common method of reusing feathers is the production of feather meal. From economic and environmental points of view, it is advantageous to develop processes to add value to this byproduct, including the extraction of keratin. Among natural biodegradable polymers, keratin-based materials have revolutionized the field of biomaterials due to their biocompatibility and biodegradability, allowing their application in biomedical, pharmaceutical, chemical, and engineering areas.

Nanocellulose-based tannin-immobilized biosorbent for efficient copper ion removal.

Tannins, which are water-soluble polyhydroxyphenols found in plant structures, offer a sustainable alternative to inorganic products for treating effluents. To overcome their solubility in water, new methods have been developed to immobilize tannin on materials such as nanocellulose, resulting in tannin-based adsorbents. This study investigates the production of different types of tannin-based adsorbents immobilized on nanocellulose nanofibrils using glutaraldehyde as a crosslinking agent. The adsorbents were characterized for their morphology, chemical composition, tannin leaching, thermal stability, and copper adsorption capacity. The main results showed that tannin leaching from the adsorbents in water was greatly reduced with glutaraldehyde. Scanning electron microscopy images showed tannin coating on the surface of nanocellulose nanofibrils due to immobilization. The lyophilized adsorbent was effective in adsorbing Cu (II) within 1 min, with the adsorbed amount increasing gradually until stabilization after 45 min. Overall, this study suggests that tannin immobilized on nanofibrillated cellulose has the potential to offer a sustainable and renewable alternative for effluent treatment.

Retinal Vascular Changes in Alzheimer's Dementia and Mild Cognitive Impairment: A Pilot Study Using Ultra-Widefield Imaging.

Purpose: Retinal microvascular abnormalities measured on retinal images are a potential source of prognostic biomarkers of vascular changes in the neurodegenerating brain. We assessed the presence of these abnormalities in Alzheimer's dementia and mild cognitive impairment (MCI) using ultra-widefield (UWF) retinal imaging. Methods: UWF images from 103 participants (28 with Alzheimer's dementia, 30 with MCI, and 45 with normal cognition) underwent analysis to quantify measures of retinal vascular branching complexity, width, and tortuosity. Results: Participants with Alzheimer's dementia displayed increased vessel branching in the midperipheral retina and increased arteriolar thinning. Participants with MCI displayed increased rates of arteriolar and venular thinning and a trend for decreased vessel branching. Conclusions: Statistically significant differences in the retinal vasculature in peripheral regions of the retina were observed among the distinct cognitive stages. However, larger studies are required to establish the clinical importance of our findings. UWF imaging may be a promising modality to assess a larger view of the retinal vasculature to uncover retinal changes in Alzheimer's disease. Translational Relevance: This pilot work reports an investigation into which retinal vasculature measurements may be useful surrogate measures of cognitive decline, as well as technical developments (e.g., measurement standardization), that are first required to establish their recommended use and translational potential.

Nonlinear decline of mini-mental state examination in Parkinson's disease.

The trajectory of cognitive functioning in patients with Parkinson's disease (PD) is not known. We used a random change point model to study the individual cognitive trajectory for up to 15 years in a prevalence sample of 238 PD patients, and used the mini-mental state examination (MMSE) to assess the longitudinal cognitive course. We observed that the rate of global cognitive decline was nonlinear. Following a relatively stable period, an inflection point was identified, after which the rate of decline gained momentum with an annual decline of 2.8 points on the MMSE. The course was similar in men and women. This inflection point was estimated to occur 13.3 years (95% credible interval 11.8, 13.6) after the diagnosis of PD; however, there were wide interindividual variations in the time from onset of PD to the inflection point.

Antioxidant, antibacterial and antitumoural activities of kraft lignin from hardwood fractionated by acid precipitation.

Kraft lignin, so far useful for energy generation, has been gathering considerable attention as an alternative material to replace fossil-based resources mainly due to its high phenolic content. However, the wide molecular weight distribution and chemical composition heterogeneity led to the development of fractionation methods. Herein, to narrow such characteristics we used eucalypt kraft lignin fractionated at pH's 9, 7, 5 and 3 by sequential acid precipitation. These lignin fractions were first characterised by simultaneous pyrolysis and trimethylsilylation (SPyT) with N-Methyl-N-(trimethylsilyl) trifluoroacetamide with posterior tests of antioxidant, antibacterial, and antitumour activities. We observed higher ratio of syringyl/guaiacyl groups and increase in antioxidant activity in those fractions with lower molecular weight (precipitated at lower pH's). Fractions precipitated at pH's 9 and 7 have shown an outstanding antibacterial activity against five bacteria. Moreover, fractions 7 and 5 presented at cytotoxicity tests higher ability to inhibit the growth of U87MG and T98G glioma cells, while only a slight inhibition of adult human fibroblasts (non-tumour cells) was detected.

Alternative methods for the pilot-scale production and characterization of chitosan nanoparticles.

This work describes the production/characterization of low molar mass chitosan nanoparticles derived from waste shrimp shells (SSC), as well as from a commercial chitosan (CC). The production of low molar mass nanochitosan employed thermal shock, alternating between 100 °C and ambient temperature, followed by grinding the dry material (SSC and CC) in a ball mill, producing around 500 g of nanochitosan per batch. A highlight of the methodology employed is that it enables nanochitosan to be obtained even from a low quality commercial raw material. All particles had diameters smaller than 223 nm, with an average diameter below 25 nm (determined by DLS), while reductions of molar mass were between 8.4-fold and 13.5-fold. The depolymerization process resulted in a reduction in crystallinity of 38.1 to 25.4% and 55.6 to 25.9% in the CC and SSC samples, respectively. The production of nanochitosans was also confirmed by TEM through the observation of crystalline domains with diameters between 5 and 10 nm. This work perfectly reproduces the results on bench scale from previous research. The simple and inexpensive processes enable easy scale-up, representing an important advance in the production chain of biopolymers. Graphical abstract.

Methylprednisolone in adults hospitalized with COVID-19 pneumonia : An open-label randomized trial (GLUCOCOVID).

PURPOSE: To determine whether a 6-day course of methylprednisolone (MP) improves outcome in patients with severe SARS-CoV­2 (Corona Virus Disease 2019 [COVID-19]). METHODS: The study was a multicentric open-label trial of COVID-19 patients who were aged ≥ 18 years, receiving oxygen without mechanical ventilation, and with evidence of systemic inflammatory response who were assigned to standard of care (SOC) or SOC plus intravenous MP (40 mg bid for 3 days followed by 20 mg bid for 3 days). The primary outcome was a composite of death, admission to the intensive care unit, or requirement for noninvasive ventilation. Both intention-to-treat (ITT) and per protocol (PP) analyses were performed. RESULTS: A total of 91 patients were screened, and 64 were randomized (mean age70 ± 12 years). In the ITT analysis, 14 of 29 patients (48%) in the SOC group and 14 of 35 (40%) in the MP group suffered the composite endpoint (40% versus 20% in patients under 72 years and 67% versus 48% in those over 72 years; p = 0.25). In the PP analysis, patients on MP had a significantly lower risk of experiencing the composite endpoint (age-adjusted risk ratio 0.42; 95% confidence interval, CI 0.20-0.89; p = 0.043). CONCLUSION: The planned sample size was not achieved, and our results should therefore be interpreted with caution. The use of MP had no significant effect on the primary endpoint in ITT analysis; however, the PP analysis showed a beneficial effect due to MP, which consistent with other published trials support the use of glucocorticoids in severe cases of COVID-19.

Feasibility of ramie fibers as raw material for the isolation of nanofibrillated cellulose.

In this study, a strategy was adopted to enhance the use of ramie fibers as raw material for isolation of cellulose nanofibers (CNFs). Ramie pulp was produced by alkaline organosolv followed by bleaching. CNFs were produced by mechanical defibrillation, and films were fabricated via casting. Effects of number of passes in the mechanical grinding on physical and mechanical properties of CNF films were comprehensively studied. Potential of ramie fibers was proved by fabricating homogeneous nanofibers with average thickness of 8.72 nm, which led to CNF films with dense and non-porous networks, and crystallinity index of 76-78%. Tensile strength (42-82 MPa) and dynamic mechanical (9-11 GPa) performance were good only for less severe mechanical defibrillation. Lower solubility (1.85-2.43%). and activity (0.69) in water, and outstanding barrier properties against water vapor and oxygen make ramie suitable for more sustainable extraction of cellulose nanofibers and production of CNF films for diverse applications.

Prediction of rapid amyloid and phosphorylated­Tau accumulation in cognitively healthy individuals.

Objective: To test the hypothesis that among cognitively healthy individuals, distinct groups exist in terms of amyloid and phosphorylated-tau accumulation rates; that if rapid accumulator groups exist, their membership can be predicted by Alzheimer's disease (AD) risk factors, and that time points of significant increase in AD protein accumulation will be evident. Methods: The analysis reports data from 263 individuals from the BIOCARD and 184 individuals from the Baltimore Longitudinal Study of Aging with repeated cerebrospinal fluid (CSF) and positron emission tomography (PET) sampling, respectively. We used latent class mixed-effect models to identify distinct classes of amyloid (CSF and PET) and p-Tau (CSF) accumulation rates and generalized additive modeling to investigate non-linear changes to AD biomarkers. Results: For both amyloid and p-Tau latent class models we confirmed the existence of two separate classes: accumulators and non-accumulators. The accumulator and non-accumulator groups differed significantly in terms of baseline AD protein levels and slope of change. APOE ε4 carrier status and episodic memory predicted amyloid class membership. Non-linear models revealed time points of significant increase in the rate of amyloid and p-Tau accumulation whereby APOE ε4 carrier status associated with earlier age at onset of rapid accumulation. Conclusions: The current analysis demonstrates the existence of distinct classes of amyloid and p-Tau accumulators. Predictors of class membership were identified but the overall accuracy of the models was modest, highlighting the need for additional biomarkers that are sensitive to early disease phenotypes.

Environmental prospecting of black yeast-like agents of human disease using culture-independent methodology.

Melanized fungi and black yeasts in the family Herpotrichiellaceae (order Chaetothyriales) are important agents of human and animal infectious diseases such as chromoblastomycosis and phaeohyphomycosis. The oligotrophic nature of these fungi enables them to survive in adverse environments where common saprobes are absent. Due to their slow growth, they lose competition with common saprobes, and therefore isolation studies yielded low frequencies of clinically relevant species in environmental habitats from which humans are thought to be infected. This problem can be solved with metagenomic techniques which allow recognition of microorganisms independent from culture. The present study aimed to identify species of the family Herpotrichiellaceae that are known to occur in Brazil by the use of molecular markers to screen public environmental metagenomic datasets from Brazil available in the Sequence Read Archive (SRA). Species characterization was performed with the BLAST comparison of previously described barcodes and padlock probe sequences. A total of 18,329 sequences was collected comprising the genera Cladophialophora, Exophiala, Fonsecaea, Rhinocladiella and Veronaea, with a focus on species related to the chromoblastomycosis. The data obtained in this study demonstrated presence of these opportunists in the investigated datasets. The used techniques contribute to our understanding of environmental occurrence and epidemiology of black fungi.

Nanocellulose obtained from residues of peach palm extraction (Bactris gasipaes).

The large amount of residues generated by the peach palm agroindustry and its cellulose content (34 g 100g-1) motivated the present investigation, where outer sheaths were used for nanocellulose production through ultrafine grinding. Protocols combining different chemical delignification with defibrillation intensity (10 and 20 cycles) were applied and their influence over some properties and characteristics of nanofibers evaluated. At all protocols applied it was possible produce cellulose at nanometric level (widths ˜100 nm), with high crystallinity index (49.8-54.5%) and great thermal stability. The delignification influenced the defibrillation, where lignin removal resulted in well dispersed bundles of fibrils with lower widths. Lower lignin contents combined with higher shear forces released more nanofibrils, with more adsorbed water with defibrillation extension. These behavior influenced the suspension stability, that was higher at larger number of cycles (20). The results evidenced that was possible produced FNC with appropriate technical characteristics from pupunha discards.

Adsorption of aquaculture pollutants using a sustainable biopolymer.

Intensive aquaculture needs to adopt techniques that are able to contribute towards sustainability. Closed systems that employ water recirculation can combine intensive production with environmental sustainability, since there is no exchange of water or discharge of effluents into the environment. In order to achieve this, effective filtration systems are required to ensure that the water quality is satisfactory for the cultivation of aquatic organisms. Chitosan, an industrial waste material derived from crustacean farming, is a renewable natural material that is biodegradable and possesses adsorbent characteristics. In this work, chitosan foam was incorporated in filters and was evaluated as an adsorbent of aquaculture pollutants, adding value to the material and at the same time providing a use for industrial waste. The foam was characterized by scanning electron microscopy and energy dispersive spectroscopy, apparent density, and water absorption capacity. It was used to remove ammonia, nitrite, orthophosphate, and turbidity from aquaculture effluents. The foam consisted of a bilayer with smooth and porous sides, which presented low density, flexibility, and high water absorption capacity. The best proportion of the foam, in terms of the mass of foam per volume of solution (% m v-1), was 0.10, which resulted in removal of 32.8, 57.2, 89.5, and 99.9% of ammonia, nitrite, orthophosphate, and turbidity, respectively. This biopolymer produced is biodegradable, and when saturated with organic compounds from aquaculture, and no longer suitable for reuse as a filter material, it can be employed as a fertilizer, hence closing the sustainability cycle of the aquaculture production chain.

Education and Successful Aging Trajectories: A Longitudinal Population-Based Latent Variable Modelling Analysis.

As the population ages, interest is increasing in studying aging well. However, more refined means of examining predictors of biopsychosocial conceptualizations of successful aging (SA) are required. Existing evidence of the relationship between early-life education and later-life SA is unclear. The Successful Aging Index (SAI) was mapped onto the Cognitive Function and Aging Study (CFAS), a longitudinal population-based cohort (n = 1,141). SAI scores were examined using growth mixture modelling (GMM) to identify SA trajectories. Unadjusted and adjusted (age, sex, occupational status) ordinal logistic regressions were conducted to examine the association between trajectory membership and education level. GMM identified a three-class model, capturing high, moderate, and low functioning trajectories. Adjusted ordinal logistic regression models indicated that individuals in higher SAI classes were significantly more likely to have higher educational attainment than individuals in the lower SAI classes. These results provide evidence of a life course link between education and SA.

Are Changes in Self-Rated Health Associated With Memory Decline in Older Adults?

OBJECTIVE: The association between patterns of change in self-rated health (SRH) and memory trajectories in older adults was examined using a systematic approach. METHOD: Data from the Health and Retirement Study ( n = 6,016) and the English Longitudinal Study of Ageing ( n = 734) were analyzed. Individuals were grouped into five categories according to their pattern of change in SRH over 8 years: stable excellent/very good/good, stable fair/poor, improvement, decline, and fluctuating pattern without a trend. Memory was measured using immediate and delayed recall tests. Kruskal-Wallis, chi-squares tests, and linear mixed models were used to examine the association. RESULTS: Different rates of decline in memory can be identified in the different patterns of change in SRH. Those who had a stable excellent/very good/good pattern had the slowest rate of decline. DISCUSSION: Our findings suggest that SRH status and patterns of change could be used as a marker of cognitive decline in prevention screening programs.

Fruit and vegetable intakes and bone mineral status: a cross sectional study in 5 age and sex cohorts.

BACKGROUND: Evidence is increasing for positive effects of fruit and vegetable intakes on bone health. However, most of the studies to date were conducted in adults, and few reports included adolescents. OBJECTIVE: We explored the association between bone mineral status and fruit and vegetable intakes in adolescent boys and girls (aged 16-18 y), young women (aged 23-37 y), and older men and women (aged 60-83 y). DESIGN: Bone mineral measurements of the whole body, hip, and spine were made in all subjects by using dual-energy X-ray absorptiometry. Information on health and lifestyle and physical activity was obtained by questionnaire. Fruit, vegetable, and nutrient intakes were ascertained from 7-d food diaries. RESULTS: In adolescent boys and girls and older women, significant positive associations were observed between spine size-adjusted bone mineral content (SA-BMC) and fruit intake. In boys only, femoral neck SA-BMC was also significantly and positively associated with the intakes of both fruit and dietary vitamin C. No significant associations were found in the young women or older men, or between bone measurements and intake of vegetables alone (after adjustments) in any of the groups. CONCLUSIONS: Higher fruit and vegetable intakes may have positive effects on bone mineral status in both younger and older age groups, especially at the spine and femoral neck. The specific mechanisms remain to be ascertained, but vitamin C, other fruit-specific antioxidants, and lifestyle may play a role.