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Biotechnol Bioeng ; 119(12): 3632-3646, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36073082

RESUMO

Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial-scale production processes and media, the CHO cell has remained largely unchanged. Due to the cost and complexity of whole-genome sequencing and gene-editing it has been difficult to obtain the tools necessary to improve the CHO cell line. With the advent of next-generation sequencing and the discovery of the CRISPR/Cas9 system it has become more cost effective to sequence and manipulate the CHO genome. Here, we provide a comprehensive de novo assembly and annotation of the CHO-K1 based CHOZN® GS-/- genome. Using this platform, we designed, built, and confirmed the functionality of a whole genome CRISPR guide RNA library that will allow the bioprocessing community to design a more robust CHO cell line leading to the production of life saving medications in a more cost-effective manner.


Assuntos
Sistemas CRISPR-Cas , Genoma , Cricetinae , Animais , Cricetulus , Células CHO , Sistemas CRISPR-Cas/genética , Genoma/genética , RNA Guia de Cinetoplastídeos/genética
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