Global patterns in endemicity and vulnerability of soil fungi.

Fungi are highly diverse organisms, which provide multiple ecosystem services. However, compared with charismatic animals and plants, the distribution patterns and conservation needs of fungi have been little explored. Here, we examined endemicity patterns, global change vulnerability and conservation priority areas for functional groups of soil fungi based on six global surveys using a high-resolution, long-read metabarcoding approach. We found that the endemicity of all fungi and most functional groups peaks in tropical habitats, including Amazonia, Yucatan, West-Central Africa, Sri Lanka, and New Caledonia, with a negligible island effect compared with plants and animals. We also found that fungi are predominantly vulnerable to drought, heat and land-cover change, particularly in dry tropical regions with high human population density. Fungal conservation areas of highest priority include herbaceous wetlands, tropical forests, and woodlands. We stress that more attention should be focused on the conservation of fungi, especially root symbiotic arbuscular mycorrhizal and ectomycorrhizal fungi in tropical regions as well as unicellular early-diverging groups and macrofungi in general. Given the low overlap between the endemicity of fungi and macroorganisms, but high conservation needs in both groups, detailed analyses on distribution and conservation requirements are warranted for other microorganisms and soil organisms.

Understanding costs and benefits of thermal plasticity for pest management: insights from the integration of laboratory, semi-field and field assessments of Ceratitis capitata (Diptera: Tephritidae).

The relative costs and benefits of thermal acclimation for manipulating field performance of pest insects depend upon a number of factors including which traits are affected and how persistent any trait changes are in different environments. By assessing plastic trait responses of Ceratitis capitata (Mediterranean fruit fly) across three distinct operational environments (laboratory, semi-field, and field), we examined the influence of different thermal acclimation regimes (cool, intermediate [or handling control], and warm) on thermal tolerance traits (chill-coma recovery, heat-knockdown time, critical thermal minimum and critical thermal maximum) and flight performance (mark-release-recapture). Under laboratory conditions, thermal acclimation altered thermal limits in a relatively predictable manner and there was a generally positive effect across all traits assessed, although some traits responded more strongly. By contrast, dispersal-related performance yielded strongly contrasting results depending on the specific operational environment assessed. In semi-field conditions, warm- or cold-acclimated flies were recaptured more often than the control group at cooler ambient conditions suggesting an overall stimulatory influence of thermal variability on low-temperature dispersal. Under field conditions, a different pattern was identified: colder flies were recaptured more in warmer field conditions relative to other treatment groups. This study highlights the trait- and context-specific nature of how thermal acclimation influences traits of thermal performance and tolerance. Consequently, laboratory and semi-field assessments of dispersal may not provide results that extend into the field setting despite the apparent continuum of environmental complexity among them (laboratory < semi-field < field).

Temperature and pH define the realised niche space of arbuscular mycorrhizal fungi.

The arbuscular mycorrhizal (AM) fungi are a globally distributed group of soil organisms that play critical roles in ecosystem function. However, the ecological niches of individual AM fungal taxa are poorly understood. We collected > 300 soil samples from natural ecosystems worldwide and modelled the realised niches of AM fungal virtual taxa (VT; approximately species-level phylogroups). We found that environmental and spatial variables jointly explained VT distribution worldwide, with temperature and pH being the most important abiotic drivers, and spatial effects generally occurring at local to regional scales. While dispersal limitation could explain some variation in VT distribution, VT relative abundance was almost exclusively driven by environmental variables. Several environmental and spatial effects on VT distribution and relative abundance were correlated with phylogeny, indicating that closely related VT exhibit similar niche optima and widths. Major clades within the Glomeraceae exhibited distinct niche optima, Acaulosporaceae generally had niche optima in low pH and low temperature conditions, and Gigasporaceae generally had niche optima in high precipitation conditions. Identification of the realised niche space occupied by individual and phylogenetic groups of soil microbial taxa provides a basis for building detailed hypotheses about how soil communities respond to gradients and manipulation in ecosystems worldwide.

Thermal limits and preferences of large branchiopods (Branchiopoda: Anostraca and Spinicaudata) from temporary wetland arid zone systems.

Large branchiopods are specialist crustaceans adapted for life in temporary, thermally dynamic wetland ecosystems. Certain large branchiopod species are, however, restricted to specific temporary wetland types, exemplified by their physico-chemical and hydroperiod characteristics. Here, we contrasted the thermal preference and critical thermal maxima (CTmax) and minima (CTmin) of southern African anostracans and spinicaudatans found exclusively in either temporary rock-pool or pan wetland types. We hypothesized that environment of origin would be a good predictor of thermal preference and critical thermal limits. To test this, Branchiopodopsis tridens (Anostraca) and Leptestheria brevirostris (Spinicaudata) were collected from rock-pool habitats, while Streptocephalus cafer (Anostraca) and a Gondwanalimnadia sp. (Spinicaudata) were collected from pan habitats. In contrast to our hypothesis, taxonomic relatedness was a better predictor of CTmax and temperature preference than environment of origin. Spinicaudatans were significantly more tolerant of high temperatures than anostracans, with L. brevirostris and Gondwanalimnadia sp. median CTmax values of 45.1 °C and 44.1 °C, respectively, followed by S. cafer (42.8 °C) and B. tridens (41.4 °C). Neither environment or taxonomic relatedness were good predictors of CTmin trends, with B. tridens (0.9 °C) and Gondwanalimnadia sp. (2.1 °C) having the lowest median CTmin values, followed by L. brevirostris (3.4 °C) and S. cafer (3.6 °C). On the contrary, temperature preferences differed according to taxa, with spinicaudatans significantly preferring higher temperatures than anostracans. Leptestheria brevirostris and Gondwanalimnadia sp. both spent most time at temperatures 30-32 °C, S. cafer at 18-20 °C and B. tridens at 21-23 °C. Constrained thermal traits reported here suggest that the studied anostracans might be more susceptible to projected climatic warming than the spinicaudatans, irrespective of habitat type, however, these taxa may also compensate through phenotypic plasticity.

Spatial Anopheles arabiensis (Diptera: Culicidae) insecticide resistance patterns across malaria-endemic regions of Botswana.

BACKGROUND: Since the advent of the Green Revolution, pesticides have played an important role in the global management of invertebrate pests including vector mosquitoes. Despite optimal efficacy, insects often display insensitivity to synthetic insecticides owing to prolonged exposure that may select for resistance development. Such insecticide insensitivity may regress national and regional coordination in mosquito vector management and indeed malaria control. In Botswana, prolonged use of synthetic insecticides against malaria vectors have been practiced without monitoring of targeted mosquito species susceptibility status. METHODS: Here, susceptibility status of a malaria vector (Anopheles arabiensis), was assessed against World Health Organization-recommended insecticides, across three malaria endemic districts. Adult virgin female mosquitoes (2-5 days old) emerging from wild-collected larvae were exposed to standardized insecticide-impregnated papers with discriminating doses. RESULTS: The results showed resistance dynamics were variable in space, presumably as a result of spatial differences in insecticide use across malaria endemic districts and the types of insecticides used in the country. Overall, there was a reduced susceptibility of An. arabiensis for the pyrethroid lambda-cyhalothrin and for dichloro diphenyl trichloroethane [DDT], which have similar modes of action and have been used in the country for many years. The Okavango district exhibited the greatest reduction in susceptibility, followed by Ngamiland and then Bobirwa, reflective of national intervention strategy spraying intensities. Vector mosquitoes were, however, highly susceptible to carbamates and organophosphates irrespective of region. CONCLUSIONS: These results provide important findings of vector susceptibility to insecticides recommended for vector control. The results highlight the need to implement insecticide application regimes that more effectively including regionally integrated resistance management strategies for effective malaria control and elimination.

Thermal plasticity in the invasive south American tomato pinworm Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae).

South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is a devastating invasive global insect pest of tomato, Solanum lycopersicum (Solanaceae). In nature, pests face multiple overlapping environmental stressors, which may significantly influence survival. To cope with rapidly changing environments, insects often employ a suite of mechanisms at both acute and chronic time-scales, thereby improving fitness at sub-optimal thermal environments. For T. absoluta, physiological responses to transient thermal variability remain under explored. Moreso, environmental effects and physiological responses may differ across insect life stages and this can have implications for population dynamics. Against this background, we investigated short and long term plastic responses to temperature of T. absoluta larvae (4th instar) and adults (24-48 h old) from field populations. We measured traits of temperature tolerance vis critical thermal limits [critical thermal minima (CTmin) and maxima (CTmax)], heat knockdown time (HKDT), chill coma recovery time (CCRT) and supercooling points (SCP). Our results showed that at the larval stage, Rapid Cold Hardening (RCH) significantly improved CTmin and HKDT but impaired SCP and CCRT. Heat hardening in larvae impaired CTmin, CCRT, SCP, CTmax but not HKDT. In adults, both heat and cold hardening generally impaired CTmin and CTmax, but had no effects on HKDT, SCP and CCRT. Low temperature acclimation significantly improved CTmin and HKDT while marginally compromising CCRT and CTmax, whereas high temperature acclimation had no significant effects on any traits except for HKDT in larvae. Similarly, low and high temperature acclimation had no effects on CTmin, SCPs and CTmax, while high temperature acclimation significantly compromised adult CCRT. Our results show that larvae are more thermally plastic than adults and can shift their thermal tolerance in short and long timescales. The larval plasticity reported here could be advantageous in new envirnments, suggesting an asymmetrical ecological role of larva relative to adults in facilitating T. absoluta invasion.

Life-stage related responses to combined effects of acclimation temperature and humidity on the thermal tolerance of Chilo partellus (Swinhoe) (Lepidoptera: Crambidae).

Adaptive thermal plasticity plays a key role in mitigating the effects of seasonal and diurnal thermal fluctuations among ectotherms at various life-stages. While the role of thermal history in conferring such plasticity is widely documented, its interaction with relative humidity (RH), another important driver of ectotherm survival and activity, is relatively underexplored. Yet the potential responses to these combinational stressors across ontogeny remain largely neglected. Against this background, we used a full-factorial design to test the combined acclimation effects of RH (45%, 65% and 85%) and temperature (23, 28 and 33 °C) on various indices of thermal sensitivity of laboratory reared spotted stemborer, Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) following acclimation beginning at larval, pupal and adult life-stages. Traits measured included critical thermal limits (CTLs), supercooling points (SCPs), chill coma recovery time (CCRT) and heat knockdown time (HKDT). Larval acclimation at 23 °C; 85% RH recorded the lowest critical thermal minima (CTmin) whereas adult acclimation at 28 °C; 45% RH recorded the highest critical thermal maxima (CTmax). There were no significant differences (P > 0.05) in SCPs across all temperature × RH acclimations. Larval and pupal acclimations at 23 °C; 85% RH and adult acclimation at 23 °C; 45% RH significantly improved CCRT. Similarly, commencing acclimation at larval, pupal and adult stages at 28 °C; 85% RH improved HKDT whereas larval and pupal acclimations at 33 °C; 45% RH impaired it. Our results indicate that combinational interactions of temperature and RH have significant thermal fitness costs and benefits and are dependent on the life-stage acclimation timing. Results also imply that both the vulnerability and adaptive potential of C. partellus populations under rapid climate variability varies with ontogeny. This therefore calls for the consideration of the role of ontogeny and multi-factors in better understanding the impact of environmental stress on ectotherms.

Food preference and foraging activity of ants: recommendations for field applications of low-toxicity baits.

Control of ants using baits of low toxicity cannot be effective without knowledge of bait distribution patterns and bait station densities, which are determined by ants' foraging activities. Furthermore, the success of toxic baits also depends upon attractiveness of bait carriers. Here, we assessed ground and vine foraging activity and food preferences for the three ant species ( Linepithema humile (Mayr) (Hymenoptera: Formicidae), Anoplolepis custodiens (F. Smith) and Crematogaster peringueyi Emery) under field conditions. We found that L. humile's vineyard foraging activity was high and that movement of ant bait by C. peringueyi and A. custodiens in the vineyard was relatively low. Consequently, more bait stations need to be dispensed for more effective control of C. peringueyi and A. custodiens than for L. humile. Different bait densities are discussed for the various ant species. Food preference trials indicated that vineyard foraging ants preferred wet bait attractants over dry ones, making liquids the most ideal carriers for baiting these ants. Linepithema humile was attracted to 25% sugar water, while C. peringueyi was attracted to both 25% sugar water and honey. Anoplolepis custodiens was attracted to tuna but was also attracted to 25% sugar water. Thus, future bait formulations should be tailor made to suit these specific food requirements if baits are to be successful in ant pest management.

The Fall Armyworm and Larger Grain Borer Pest Invasions in Africa: Drivers, Impacts and Implications for Food Systems.

Invasive alien species (IAS) are a major biosecurity threat affecting globalisation and the international trade of agricultural products and natural ecosystems. In recent decades, for example, field crop and postharvest grain insect pests have independently accounted for a significant decline in food quantity and quality. Nevertheless, how their interaction and cumulative effects along the ever-evolving field production to postharvest continuum contribute towards food insecurity remain scant in the literature. To address this within the context of Africa, we focus on the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), and the larger grain borer, Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae), two of the most important field and postharvest IAS, respectively, that have invaded Africa. Both insect pests have shown high invasion success, managing to establish themselves in >50% of the African continent within a decade post-introduction. The successive and summative nature of field and postharvest damage by invasive insect pests on the same crop along its value chain results in exacerbated food losses. This systematic review assesses the drivers, impacts and management of the fall armyworm and larger grain borer and their effects on food systems in Africa. Interrogating these issues is important in early warning systems, holistic management of IAS, maintenance of integral food systems in Africa and the development of effective management strategies.

Opportunities to improve goat production and food security in Botswana through forage nutrition and the use of supplemental feeds.

Goats fulfil a central role in food and nutritional security across Africa with over half of households owning or rearing goats in rural areas. However, goat performance is poor and mortality high. This study assessed the nutritional quality of commonly used feeds and proposes feed-baskets to enhance goat nutrition and health. Feeds were collected from 11 areas within the Central District of Botswana, and macronutrient analyses were conducted, including crude protein, fibre fractions, ash, and metabolizable energy (ME). Forage nutrition was compared across seasons and soil types. Additionally, seasonal supplementation trials were conducted to evaluate consumption rates of various supplements, including crop residues, pellets, Lablab purpureus, and Dichrostachys cinerea. Each supplement was provided ad libitum for a 24-h period, and consumption rates determined. Findings revealed significant differences in nutrition among various feed sources, across seasons, and in relation to soil types (p < 0.001). Consumption rates of supplements were higher during the dry season, possibly due to reduced forage availability. Supplement consumption rates varied across supplement type, with crop residues accounting for approximately 1% of dry matter intake, compared to up to 45% for pellets, 13% for L. purpureus, and 15% for D. cinerea. While wet season feed baskets exhibited higher ME values compared to dry-season feed-baskets, the relative impact of supplementation was more pronounced during the dry season. These results highlight the potential for optimizing goat diets through improved grazing and browsing management, especially during the reduced nutritional availability in the dry season in Botswana. Such diet optimisation may improve goat health and productivity, which may positively impact the food and financial security of smallholders by providing both increased yields and increased resilience. Importantly, rural communities can experience some of the lowest food security levels in the region. The interventions explored in this study utilise natural capital, often freely available, which can be deployed through existing husbandry systems, potentially making them accessible and practical to smallholders.

Sub-optimal host plants have developmental and thermal fitness costs to the invasive fall armyworm.

The fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) is a global invasive pest of cereals. Although this pest uses maize and sorghum as its main hosts, it is associated with a wide range of host plants due to its polyphagous nature. Despite the FAW's polyphagy being widely reported in literature, few studies have investigated the effects of the non-preferred conditions or forms (e.g., drought-stressed forms) of this pest's hosts on its physiological and ecological fitness. Thus, the interactive effects of biotic and abiotic stresses on FAW fitness costs or benefits have not been specifically investigated. We therefore assessed the effects of host plant quality on the developmental rates and thermal tolerance of the FAW. Specifically, we reared FAW neonates on three hosts (maize, cowpeas, and pearl millet) under two treatments per host plant [unstressed (well watered) and stressed (water deprived)] until the adult stage. Larval growth rates and pupal weights were determined. Thermal tolerance traits viz critical thermal maxima (CTmax), critical thermal minima (CTmin), heat knockdown time (HKDT), chill-coma recovery time (CCRT), and supercooling points (SCPs) were measured for the emerging adults from each treatment. The results showed that suboptimal diets significantly prolonged the developmental time of FAW larvae and reduced their growth rates and ultimate body weights, but did not impair their full development. Suboptimal diets (comprising non-cereal plants and drought-stressed cereal plants) increased the number of larval instars to eight compared to six for optimal natural diets (unstressed maize and pearl millet). Apart from direct effects, in all cases, suboptimal diets significantly reduced the heat tolerance of FAWs, but their effect on cold tolerance was recorded only in select cases (e.g., SCP). These results suggest host plant effects on the physical and thermal fitness of FAW, indicating a considerable degree of resilience against multiple stressors. This pest's resilience can present major drawbacks to its cultural management using suboptimal hosts (in crop rotations or intercrops) through its ability to survive on most host plants despite their water stress condition and gains in thermal fitness. The fate of FAW population persistence under multivariate environmental stresses is therefore not entirely subject to prior environmental host plant history or quality.

Connecting the multiple dimensions of global soil fungal diversity.

How the multiple facets of soil fungal diversity vary worldwide remains virtually unknown, hindering the management of this essential species-rich group. By sequencing high-resolution DNA markers in over 4000 topsoil samples from natural and human-altered ecosystems across all continents, we illustrate the distributions and drivers of different levels of taxonomic and phylogenetic diversity of fungi and their ecological groups. We show the impact of precipitation and temperature interactions on local fungal species richness (alpha diversity) across different climates. Our findings reveal how temperature drives fungal compositional turnover (beta diversity) and phylogenetic diversity, linking them with regional species richness (gamma diversity). We integrate fungi into the principles of global biodiversity distribution and present detailed maps for biodiversity conservation and modeling of global ecological processes.

Density-dependent ecosystem service delivery under shifting temperatures by dung beetles.

Increases in the frequency and magnitude of suboptimal temperatures as a result of climate change are subjecting insects to unprecedented stresses. This may negatively affect their fitness and the efficiency of their ecosystem service provision. Dung beetles are ecosystem service providers: through feeding on and burying dung, they facilitate nutrient recycling, secondary seed dispersal, parasite control, soil bioturbation and dung decomposition. As such, prediction of how dung beetles respond to multiple anthropogenic environmental changes is critical for the conservation of ecosystem services. Here, we quantified ecosystem services via dung utilisation and dung ball production in three telecoprid species: Allogymnopleurus indigaceous, Scarabaeus zambezianus and Khepher prodigiosus. We examined ecosystem service efficiency factorially under different beetle densities towards different dung masses and under three temperature treatments (21 °C, 28 °C and 35 °C). Khepher prodigiosus, exhibited greatest dung utilisation efficiency overall across dung masses, compared to both S. zambezianus and A. indigaceous. Dung removal was exhibited under all the tested temperatures by all tested species, and therefore the sub-optimal temperatures employed here did not fully inhibit ecosystem service delivery. However, emergent effects among temperatures, beetle species and beetle density further affected removal efficiency: S. zambezianus and A. indigaceous utilisation increased with both warming and beetle density, whereas K. prodigiosus performance was less temperature- and density-dependent. Beetles also tended to exhibit positive density-dependence as dung supply increased. The numbers of dung balls produced differed across species, and increased with temperature and densities, with S. zambezianus producing significantly most balls overall. Our study provides novel evidence for differential density-dependent ecosystem service delivery among species across stressful temperature regimes and emergent effects for dung mass utilisation. This information is essential for biodiversity-ecosystem-function and is critical for the conservation of functionally efficacious species, with implications for natural capital conservation policy in rapidly changing environments.

Context-dependent integrated stress resistance promotes a global invasive pest.

In nature, insects concurrently face multiple environmental stressors, a scenario likely increasing with climate change. Integrated stress resistance (ISR) thus often improves fitness and could drive invasiveness, but how physiological mechanisms influence invasion has lacked examination. Here, we investigated cross-tolerance to abiotic stress factors which may influence range limits in the South American tomato pinworm-a global invader that is an ecologically and socially damaging crop pest. Specifically, we tested the effects of prior rapid cold- and heat-hardening (RCH and RHH), fasting, and desiccation on cold and heat tolerance traits, as well as starvation and desiccation survivability between T. absoluta life stages. Acclimation effects on critical thermal minima (CTmin ) and maxima (CTmax ) were inconsistent, showing significantly deleterious effects of RCH on adult CTmax and CTmin and, conversely, beneficial acclimation effects of RCH on larval CTmin . While no beneficial effects of desiccation acclimation were recorded for desiccation tolerance, fasted individuals had significantly higher survival in adults, whereas fasting negatively affected larval tolerances. Furthermore, fasted and desiccation acclimated adults had significantly higher starvation tolerance, showing strong evidence for cross-tolerance. Our results show context-dependent ISR traits that may promote T. absoluta fitness and competitiveness. Given the frequent overlapping occurrence of these divergent stressors, ISR reported here may thus partly elucidate the observed rapid global spread of T. absoluta into more stressful environments than expected. This information is vital in determining the underpinnings of multistressor responses, which are fundamental in forecasting species responses to changing environments and management responses.

Biogeography of cereal stemborers and their natural enemies: forecasting pest management efficacy under changing climate.

BACKGROUND: Climate warming presents physiological challenges to insects, manifesting as loss of key life-history fitness traits and survival. For interacting host-parasitoid species, physiological responses to heat stress may vary, thereby potentially uncoupling trophic ecological relationships. Here, we assessed heat tolerance traits and sensitivity to prevailing and future maximum temperatures for the cereal stemborer pests, Chilo partellus, Busseola fusca and Sesamia calamistis and their endo-parasitoids, Cotesia sesamiae and Cotesia flavipes. We further used the machine learning algorithm, Maximum Entropy (MaxEnt), to model current and potential distribution of these species. RESULTS: The mean critical thermal maxima (CTmax ) ranged from 39.5 ± 0.9°C to 44.6 ± 0.6°C and from 46.8 ± 0.7°C to 48.5 ± 0.9°C for parasitoids and stemborers, with C. sesamiae and Ch. partellus exhibiting the lowest and highest CTmax respectively. From the current climate to the 2050s scenario, parasitoids recorded a significant reduction in warming tolerance compared with their hosts. Habitat suitability for all stemborer-parasitoid species was spatially heterogeneous under current and future climatic scenarios. Cotesia sesamiae C. flavipes and B. fusca exhibited significant habitat loss, whereas Ch. partellus and S. calamistis showed a significant habitat gain under future 2050s predictions. Model metrics based on mean area under the curve ranged from 0.72 to 0.84 for all species, indicating a good predictive performance of the models. CONCLUSION: These results suggest C. sesamiae and C. flavipes may face survival constraints or extirpation compared with their pest hosts when environmental temperature reaches their upper thermal limits earlier, likely reducing pest regulation through density-mediated effects. The results demonstrate potential destabilization of stemborer-parasitoid trophic systems potentially compromising biocontrol efficacy under climate warming. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Geographic dispersion of invasive crop pests: the role of basal, plastic climate stress tolerance and other complementary traits in the tropics.

Global pest invasions have significantly increased in recent years. These invasions together with climate warming directly impact agriculture. Tropical climates feature extreme weather events, including high temperatures and seasonal droughts. Thus, successful invasive pests in tropics have to adapt to these extreme climate features. The intrinsic factors relevant to tropical invasion of insects have been explored in many studies, but the knowledge is rather dispersed in contemporary literature. Here, we reviewed the potential biophysical characters of successful invasive pests' adaption to tropical environments including [1] inherent high basal stress tolerance and advanced life-history performances [2], phenotypic plasticity [3], rapid evolution to environmental stress, polyphagy, diverse reproductive strategies and high fecundity. We summarised how these traits and their interactive effects enhance pest invasions in the tropics. Comprehensive understanding of how these characters facilitate invasion improves models for predicting ecological consequences of climate change on invasive pest species for improved pest management.

Limited thermal plasticity may constrain ecosystem function in a basally heat tolerant tropical telecoprid dung beetle, Allogymnopleurus thalassinus (Klug, 1855).

Tropical organisms are more vulnerable to climate change and associated heat stress as they live close to their upper thermal limits (UTLs). UTLs do not only vary little across tropical species according to the basal versus plasticity 'trade-off' theory but may also be further constrained by low genetic variation. We tested this hypothesis, and its effects on ecosystem function using a diurnally active dung rolling beetle (telecoprid), Allogymnopleurus thalassinus (Klug, 1855) that inhabits arid environments. Specifically, (i) we tested basal heat tolerance (critical thermal maxima [CTmax] and heat knockdown time [HKDT]), and (ii) ecological functioning (dung removal) efficiency following dynamic chronic acclimation temperatures of variable high (VT-H) (28-45 °C) and variable low (VT-L) (28-16 °C). Results showed that A. thalassinus had extremely high basal heat tolerance (> 50 °C CTmax and high HKDT). Effects of acclimation were significant for heat tolerance, significantly increasing and reducing CTmax values for variable temperature high and variable temperature low respectively. Similarly, effects of acclimation on HKDT were significant, with variable temperature high significantly increasing HKDT, while variable temperature low reduced HKDT. Effects of acclimation on ecological traits showed that beetles acclimated to variable high temperatures were ecologically more efficient in their ecosystem function (dung removal) compared to those acclimated at variable low temperatures. Allogymnopleurus thalassinus nevertheless, had low acclimation response ratios, signifying limited scope for complete plasticity for UTLs tested here. This result supports the 'trade-off' theory, and that observed limited plasticity may unlikely buffer A. thalassinus against effects of climate change, and by extension, albeit with caveats to other tropical ecological service providing insect species. This work provides insights on the survival mechanisms of tropical species against heat and provides a framework for the conservation of these natural capital species that inhabit arid environments under rapidly changing environmental climate.

Disease Vector Relative Spatio-Temporal Abundances to Water Bodies and Thermal Fitness Across Malaria Endemic Semi-Arid Areas.

The biophysical environment plays an important role in the spatio-temporal abundance and distribution of mosquitoes. This has implications for the spread of vectors and diseases they cause across diverse landscapes. Here, we assessed vector mosquito abundances in relation to large water bodies, from three malaria districts in a semi-arid environment. Furthermore, we explored thermal limits to activity of the dominant and most medically important malaria vector across malaria-endemic areas. Mosquitoes were trapped near permanent water bodies across different districts. Critical thermal limits (critical thermal-maxima and -minima) to activity of wild adults and 4th instar larvae Anopheles arabiensis (Diptera: Culicidae) were assessed. Our results showed that Anopheles spp. dominate mosquito communities across all three districts, but that their numbers were far greater in Okavango than in other regions. At the Okavango sites, the numbers of Anopheles spp. decreased with distance from main water source. Anopheles spp. sampled in this region comprised Anopheles gambiae (Giles,1902) and Anopheles funestus (Giles, 1900) species complexes, with the former dominating in numbers. Thermal activity assays showed An. arabiensis females had wider thermal tolerance windows than males while larval thermal activity limits differed significantly across space. These results confirm that the Okavango district should be prioritized for vector control measures. Moreover, intervention strategies should consider recommendations for proximity effects to large water bodies, given the differential risk associated with distance from water. The wider thermal window on female vectors has implications for possible future malaria transmission and diverse habitat utilization under changing environments.

Cold hardiness of the South American tomato pinworm Tuta absoluta (Lepidoptera: Gelechiidae): both larvae and adults are chill-susceptible.

BACKGROUND: For many insects, including invasive species, overwintering survival is achieved behaviourally (e.g. through migration) or physiologically by entering diapause, a state of arrested physiological development that may be accompanied with depressed supercooling points (SCPs). Diapause allows in situ adaptation to adverse environmental conditions, providing sufficient parent propagules for insect pest proliferation when optimal conditions resurface. This phenomenon has however not been observed in the invasive South American tomato pinworm Tuta absoluta in its Mediterranean invaded areas. Moreover, no studies have looked at its overwintering survival in sub-Saharan Africa. Here, we thus investigated the cold hardiness of Tuta absoluta larvae and adults to better explain its local overwintering adaptation strategy. RESULTS: Larval lower lethal temperatures ranged from -1 to -17 °C for 0.5 to 4 h durations. Adults showed lower temperature activity limits than larvae albeit freeze strategy experiments showed neither survived internal freezing. Fasting and dehydration pre-treatment generally depressed SCPs, although asymmetrically, conferring more negative SCPs for larvae. Ramping rates, synonymic to diurnal temperature changes also significantly affected SCPs while, inoculative freezing significantly compromised freezing temperatures in both larvae and adults. CONCLUSION: Our results suggest that (i) Tuta absoluta larvae and adults are chill-susceptible and may successfully overwinter, (ii) larvae appear more cold hardy than adults and (iii) ecological factors e.g. inoculative freezing, cooling rates, feeding- and hydration-status may affect cold hardiness. These results are important in determining species range limits, population phenology, modelling pest risk status and allows temporal life-stage specific targeting of management strategies.

Heat tolerance may determine activity time in coprophagic beetle species (Coleoptera: Scarabaeidae).

Although reports have documented loss of species diversity and ecological services caused by stressful temperature changes that result from climate change, some species cope through behavioral compensation. As temperatures and magnitudes of temperature extremes increase, animals should compensate to maintain fitness (such as through temporary behavioral shifts in activity times). Appropriate timing of activity helps avoid competition across species. Although coprophagic dung beetles exhibit species-specific temporal activity times, it is unknown whether temperature drives evolution of these species-specific temporal activity times. Using nine dung beetle species (three each of diurnal, crepuscular, and nocturnal species), we explored differences in heat stress tolerance measured as critical thermal maxima (CTmax ; the highest temperature allowing activity) and heat knockdown time (HKDT; survival time under acute heat stress) across these species, and examined the results using a phylogenetically informed approach. Our results showed that day-active species had significantly higher CTmax (diurnal > crepuscular = nocturnal species), whereas crepuscular species had higher HKDT (crepuscular > nocturnal > diurnal species). There was no correlation between heat tolerance and body size across species with distinct temporal activity, and no significant phylogenetic constraint for activity. Species with higher CTmax did not necessarily have higher HKDT, which indicates that species may respond differently to diverse heat tolerance metrics. Acute heat tolerance for diurnal beetles indicates that this trait may constrain activity time and, under high acute temperatures with climate change, species may shift activity times in more benign environments. These results contribute to elucidate the evolution of foraging behavior and management of coprophagic beetle ecosystem services under changing environments.