[A Large Number of Circulating Tumor Cells(CTCs)Can Be Isolated from Samples Obtained by Using Leukapheresis Procedures].

We hypothesized that a large number of circulating tumor cells(CTCs)may be isolated from samples obtained by using the leukapheresis procedures that are utilized to collect peripheral blood mononuclear cells for dendritic cell vaccine therapy. We utilized the CellSearch System to determine the number of CTCs in samples obtained by using leukapheresis in 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In all patients, a large number of CTCs were isolated. The mean number of CTCs per tumor was 17.1(range 10-34)in colorectal cancer, 10.0(range 2-27)in breast cancer, and 24.0(range 2-42)in gastric cancer. We succeeded in culturing the isolated CTCs from 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In conclusion, compared to conventional methods, a large number of CTCs can be obtained by using leukapheresis procedures. The molecular analyses of the CTCs isolated by using this method should be promising in the development of personalized cancer treatments.

Cholangiographic criteria for anomalous union of the pancreatic and biliary ducts.

BACKGROUND: Anomalous union of the pancreatic and biliary ducts (AUPBD) is characterized by the intersection of these ducts proximal to the sphincter of Oddi. Thus, the relative anatomic relationship between the pancreaticobiliary ductal union and the narrow distal segment of the bile duct, which represents the functional sphincter, on cholangiopancreatograms determines whether the ductal union is normal or anomalous. This study examined whether the length of the common channel or that of the narrow distal segment is more closely associated with AUPBD and defined radiographic criteria for AUPBD. METHODS: The length of the common channel and of the narrow distal segment were measured directly on cholangiopancreatograms from 350 patients. RESULTS: AUPBD was found in 36 patients (10%). The common channel was significantly longer in patients with AUPBD (p < 0.001), whereas the length of the narrow distal segment was comparable between patients with and without AUPBD (p = 0.38). There was considerable overlap in the range of the length of the common channel between patients with and without AUPBD. CONCLUSIONS: The length of the common channel is closely associated with the presence of AUPBD. However, a diagnosis of AUPBD based on the length of the common channel is not reliable. The diagnosis of AUPBD continues to rely on the radiographic criterion of a pancreaticobiliary ductal union located proximal to the narrow distal segment, which represents the sphincter of Oddi.

Primary jejunal malignant melanoma first noticed because of the presence of parotid lymph node metastasis.

The case of a 71-year-old Japanese man with primary jejunal melanoma with an unusual clinical course is reported. Several cases of primary malignant melanoma of the small intestine have been reported, but in most cases no clear evidence has shown whether the lesions were primary or metastatic. We discuss why the tumor in our case was considered primary, and we review similar cases of primary melanoma of the small intestine.