RESUMO
Background: The safety and effectiveness of dual therapy (direct oral anticoagulant [DOAC] plus P2Y12 inhibitor) versus triple therapy (vitamin K antagonist plus aspirin and P2Y12 inhibitor) in patients with nonvalvular atrial fibrillation (AF) after percutaneous coronary intervention (PCI) is unclear. Purpose: To examine the effects of dual versus triple therapy on bleeding and ischemic outcomes in adults with AF after PCI. Data Sources: Searches of PubMed, EMBASE, and the Cochrane Library (inception to 31 December 2019) and ClinicalTrials.gov (7 January 2020) without language restrictions; journal Web sites; and reference lists. Study Selection: Randomized controlled trials that compared the effects of dual versus triple therapy on bleeding, mortality, and ischemic events in adults with AF after PCI. Data Extraction: Two independent investigators abstracted data, assessed the quality of evidence, and rated the certainty of evidence. Data Synthesis: Four trials encompassing 7953 patients were selected. At the median follow-up of 1 year, high-certainty evidence showed that dual therapy was associated with reduced risk for major bleeding compared with triple therapy (risk difference [RD], -0.013 [95% CI, -0.025 to -0.002]). Low-certainty evidence showed inconclusive effects of dual versus triple therapy on risks for all-cause mortality (RD, 0.004 [CI, -0.010 to 0.017]), cardiovascular mortality (RD, 0.001 [CI, -0.011 to 0.013]), myocardial infarction (RD, 0.003 [CI, -0.010 to 0.017]), stent thrombosis (RD, 0.003 [CI, -0.005 to 0.010]), and stroke (RD, -0.003 [CI, -0.010 to 0.005]). The upper bounds of the CIs for these effects were compatible with possible increased risks with dual therapy. Limitation: Heterogeneity of study designs, dosages of DOACs, and types of P2Y12 inhibitors. Conclusion: In adults with AF after PCI, dual therapy reduces risk for bleeding compared with triple therapy, whereas its effects on risks for death and ischemic end points are still unclear. Primary Funding Source: None.
Assuntos
Síndrome Coronariana Aguda/cirurgia , Fibrilação Atrial/tratamento farmacológico , Intervenção Coronária Percutânea , Terapia Trombolítica/métodos , Aspirina/uso terapêutico , Quimioterapia Combinada , Fibrinolíticos/uso terapêutico , Humanos , Inibidores da Agregação Plaquetária/uso terapêutico , Complicações Pós-Operatórias/tratamento farmacológico , Ensaios Clínicos Controlados Aleatórios como AssuntoRESUMO
OBJECTIVE: We previously showed that cholesterol loading in vitro converts mouse aortic vascular smooth muscle cells (VSMC) from a contractile state to one resembling macrophages. In human and mouse atherosclerotic plaques, it has become appreciated that ≈40% of cells classified as macrophages by histological markers may be of VSMC origin. Therefore, we sought to gain insight into the molecular regulation of this clinically relevant process. APPROACH AND RESULTS: VSMC of mouse (or human) origin were incubated with cyclodextrin-cholesterol complexes for 72 hours, at which time the expression at the protein and mRNA levels of contractile-related proteins was reduced and of macrophage markers increased. Concurrent was downregulation of miR-143/145, which positively regulate the master VSMC differentiation transcription factor myocardin. Mechanisms were further probed in mouse VSMC. Maintaining the expression of myocardin or miR-143/145 prevented and reversed phenotypic changes caused by cholesterol loading. Reversal was also seen when cholesterol efflux was stimulated after loading. Notably, despite expression of macrophage markers, bioinformatic analyses showed that cholesterol-loaded cells remained closer to the VSMC state, consistent with impairment in classical macrophage functions of phagocytosis and efferocytosis. In apoE-deficient atherosclerotic plaques, cells positive for VSMC and macrophage markers were found lining the cholesterol-rich necrotic core. CONCLUSIONS: Cholesterol loading of VSMC converts them to a macrophage-appearing state by downregulating the miR-143/145-myocardin axis. Although these cells would be classified by immunohistochemistry as macrophages in human and mouse plaques, their transcriptome and functional properties imply that their contributions to atherogenesis would not be those of classical macrophages.
Assuntos
Transdiferenciação Celular , Colesterol/metabolismo , Células Espumosas/metabolismo , MicroRNAs/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Proteínas Nucleares/metabolismo , Transativadores/metabolismo , Animais , Aorta Torácica/metabolismo , Aorta Torácica/patologia , Apolipoproteínas E/deficiência , Apolipoproteínas E/genética , Aterosclerose/genética , Aterosclerose/metabolismo , Aterosclerose/patologia , Sítios de Ligação , Linhagem da Célula , HDL-Colesterol/metabolismo , Técnicas de Cocultura , Modelos Animais de Doenças , Células Espumosas/patologia , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica , Humanos , Células Jurkat , Camundongos Endogâmicos C57BL , Camundongos Knockout , MicroRNAs/genética , Músculo Liso Vascular/patologia , Miócitos de Músculo Liso/patologia , Necrose , Proteínas Nucleares/genética , Análise de Sequência com Séries de Oligonucleotídeos , Fagocitose , Fenótipo , Placa Aterosclerótica , Transdução de Sinais , Proteína de Ligação a Elemento Regulador de Esterol 2/metabolismo , Fatores de Tempo , Transativadores/genética , TransfecçãoRESUMO
Punta Toro virus (PTV), a member of the PTV complex, is a relatively common causative agent of febrile illness in Panama that is often misdiagnosed as 'dengue' or 'influenza'. Currently, only two named members make up this species complex, PTV and Buenaventura virus (BUEV). Genomic and antigenic characterization of 17 members of the PTV complex, nine of which were isolated from human acute febrile illness cases, reveals that this species complex is composed of six distant viruses. We propose to add four additional new viruses, designated Leticia virus, Cocle virus, Campana virus and Capira virus.
Assuntos
Infecções por Bunyaviridae/virologia , Febre/virologia , Phlebovirus/isolamento & purificação , Animais , Anticorpos Antivirais , Infecções por Bunyaviridae/imunologia , Reações Cruzadas , Febre/imunologia , Humanos , Insetos Vetores/virologia , Dados de Sequência Molecular , Panamá , Phlebovirus/classificação , Phlebovirus/genética , Phlebovirus/imunologia , Filogenia , Psychodidae/virologiaRESUMO
Most alphaviruses and many other arboviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, humans, and nonhuman primates. Consequently, they can be propagated in most vertebrate and insect cell cultures. This ability of arboviruses to infect arthropods and vertebrates is usually essential for their maintenance in nature. However, several flaviviruses have recently been described that infect mosquitoes but not vertebrates, although the mechanism of their host restriction has not been determined. Here we describe a unique alphavirus, Eilat virus (EILV), isolated from a pool of Anopheles coustani mosquitoes from the Negev desert of Israel. Phylogenetic analyses placed EILV as a sister to the Western equine encephalitis antigenic complex within the main clade of mosquito-borne alphaviruses. Electron microscopy revealed that, like other alphaviruses, EILV virions were spherical, 70 nm in diameter, and budded from the plasma membrane of mosquito cells in culture. EILV readily infected a variety of insect cells with little overt cytopathic effect. However, in contrast to typical mosquito-borne alphaviruses, EILV could not infect mammalian or avian cell lines, and viral as well as RNA replication could not be detected at 37 °C or 28 °C. Evolutionarily, these findings suggest that EILV lost its ability to infect vertebrate cells. Thus, EILV seems to be mosquito-specific and represents a previously undescribed complex within the genus Alphavirus. Reverse genetic studies of EILV may facilitate the discovery of determinants of alphavirus host range that mediate disease emergence.
Assuntos
Alphavirus/genética , Alphavirus/fisiologia , Anopheles/virologia , Evolução Biológica , Interações Hospedeiro-Patógeno/fisiologia , Filogenia , Replicação Viral/fisiologia , Alphavirus/ultraestrutura , Animais , Sequência de Bases , Teorema de Bayes , Clonagem Molecular , Análise por Conglomerados , Eletroforese em Gel de Ágar , Israel , Funções Verossimilhança , Microscopia Eletrônica de Transmissão , Modelos Genéticos , Dados de Sequência Molecular , RNA Viral/genética , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Genomic and antigenic characterization of members of the Sandfly fever Naples virus (SFNV) complex reveals the presence of five clades that differ in their geographical distribution. Saint Floris and Gordil viruses, both found in Africa, form one clade; Punique, Granada and Massilia viruses, all isolated in the western Mediterranean, constitute a second; Toscana virus, a third; SFNV isolates from Italy, Cyprus, Egypt and India form a fourth; while Tehran virus and a Serbian isolate Yu 8/76, represent a fifth. Interestingly, this last clade appears not to express the second non-structural protein ORF. Karimabad virus, previously classified as a member of the SFNV complex, and Gabek Forest virus are distinct and form a new species complex (named Karimabad) in the Phlebovirus genus. In contrast with the high reassortment frequency observed in some South American phleboviruses, the only virus of the SFNV complex with evidence of reassortment was Granada virus.
Assuntos
Febre por Flebótomos/virologia , Phlebovirus/classificação , Phlebovirus/genética , Filogeografia , RNA Viral/genética , Humanos , Dados de Sequência Molecular , Phlebovirus/isolamento & purificação , Vírus Reordenados/classificação , Vírus Reordenados/genética , Recombinação Genética , Análise de Sequência de DNARESUMO
A thorough characterization of the genetic diversity of viruses present in vector and vertebrate host populations is essential for the early detection of and response to emerging pathogenic viruses, yet genetic characterization of many important viral groups remains incomplete. The Simbu serogroup of the genus Orthobunyavirus, family Bunyaviridae, is an example. The Simbu serogroup currently consists of a highly diverse group of related arboviruses that infect both humans and economically important livestock species. Here, we report complete genome sequences for 11 viruses within this group, with a focus on the large and poorly characterized Manzanilla and Oropouche species complexes. Phylogenetic and pairwise divergence analyses indicated the presence of high levels of genetic diversity within these two species complexes, on a par with that seen among the five other species complexes in the Simbu serogroup. Based on previously reported divergence thresholds between species, the data suggested that these two complexes should actually be divided into at least five species. Together these five species formed a distinct phylogenetic clade apart from the rest of the Simbu serogroup. Pairwise sequence divergences among viruses of this clade and viruses in other Simbu serogroup species complexes were similar to levels of divergence among the other orthobunyavirus serogroups. The genetic data also suggested relatively high levels of natural reassortment, with three potential reassortment events present, including two well-supported events involving viruses known to infect humans.
Assuntos
Genoma Viral , Orthobunyavirus/classificação , Orthobunyavirus/genética , Filogenia , RNA Viral/genética , Análise de Sequência de DNA , Análise por Conglomerados , Variação Genética , Dados de Sequência MolecularRESUMO
Evolutionary insights into the phleboviruses are limited because of an imprecise classification scheme based on partial nucleotide sequences and scattered antigenic relationships. In this report, the serologic and phylogenetic relationships of the Uukuniemi group viruses and their relationships with other recently characterized tick-borne phleboviruses are described using full-length genome sequences. We propose that the viruses currently included in the Uukuniemi virus group be assigned to five different species as follows: Uukuniemi virus, EgAn 1825-61 virus, Fin V707 virus, Chizé virus, and Zaliv Terpenia virus would be classified into the Uukuniemi species; Murre virus, RML-105-105355 virus, and Sunday Canyon virus would be classified into a Murre virus species; and Grand Arbaud virus, Precarious Point virus, and Manawa virus would each be given individual species status. Although limited sequence similarity was detected between current members of the Uukuniemi group and Severe fever with thrombocytopenia syndrome virus (SFTSV) and Heartland virus, a clear serological reaction was observed between some of them, indicating that SFTSV and Heartland virus should be considered part of the Uukuniemi virus group. Moreover, based on the genomic diversity of the phleboviruses and given the low correlation observed between complement fixation titers and genetic distance, we propose a system for classification of the Bunyaviridae based on genetic as well as serological data. Finally, the recent descriptions of SFTSV and Heartland virus also indicate that the public health importance of the Uukuniemi group viruses must be reevaluated.
Assuntos
Vírus Uukuniemi/classificação , Genoma Viral , Genótipo , RNA Viral/genética , Análise de Sequência de DNA , Sorotipagem , Vírus Uukuniemi/genética , Vírus Uukuniemi/imunologiaRESUMO
Six novel insect-specific viruses, isolated from mosquitoes and phlebotomine sand flies collected in Brazil, Peru, the United States, Ivory Coast, Israel, and Indonesia, are described. Their genomes consist of single-stranded, positive-sense RNAs with poly(A) tails. By electron microscopy, the virions appear as spherical particles with diameters of â¼45 to 55 nm. Based on their genome organization and phylogenetic relationship, the six viruses, designated Negev, Ngewotan, Piura, Loreto, Dezidougou, and Santana, appear to form a new taxon, tentatively designated Negevirus. Their closest but still distant relatives are citrus leposis virus C (CiLV-C) and viruses in the genus Cilevirus, which are mite-transmitted plant viruses. The negeviruses replicate rapidly and to high titer (up to 10(10) PFU/ml) in mosquito cells, producing extensive cytopathic effect and plaques, but they do not appear to replicate in mammalian cells or mice. A discussion follows on their possible biological significance and effect on mosquito vector competence for arboviruses.
Assuntos
Anopheles/virologia , Culex/virologia , Vírus de Insetos/classificação , Phlebotomus/virologia , Vírus de RNA/classificação , Animais , Sequência de Bases , Linhagem Celular , Chlorocebus aethiops/virologia , Cricetinae , Drosophila melanogaster/virologia , Vírus de Insetos/genética , Vírus de Insetos/isolamento & purificação , Filogenia , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , RNA Viral , Análise de Sequência de RNA , Células Vero , Replicação ViralRESUMO
Genomic and antigenic characterization of the Salehabad virus, a species of the genus Phlebovirus, and four other unclassified phleboviruses (Arbia, Adria, Arumowot and Odrenisrou) demonstrate a serological and genetic relation to one another and are distinct from the eight other recognized species within the genus Phlebovirus. We propose to incorporate these four unclassified viruses as part of the Salehabad species complex within the genus. The known geographical distribution for the members of this species group includes southern Europe, Central Asia and Africa.
Assuntos
Antígenos Virais/análise , Genoma Viral , Phlebovirus/química , Phlebovirus/genética , RNA Viral/genética , Análise de Sequência de DNA , África , Ásia Central , Análise por Conglomerados , Europa (Continente) , Dados de Sequência Molecular , Phlebovirus/classificação , Phlebovirus/isolamento & purificação , Filogeografia , Vírus não ClassificadosRESUMO
Globally, yellow fever virus infects nearly 200,000 people, leading to 30,000 deaths annually. Although the virus is endemic to Latin America, only a single genome from this region has been sequenced. Here, we report 12 Brazilian yellow fever virus complete genomes, their genetic traits, phylogenetic characterization, and phylogeographic dynamics. Variable 3' noncoding region (3'NCR) patterns and specific mutations throughout the open reading frame altered predicted secondary structures. Our findings suggest that whereas the introduction of yellow fever virus in Brazil led to genotype I-predominant dispersal throughout South and Central Americas, genotype II remained confined to Bolivia, Peru, and the western Brazilian Amazon.
Assuntos
Genoma Viral , Filogenia , Vírus da Febre Amarela/genética , Sequência de Bases , Brasil , Primers do DNA , Glicosilação , Reação em Cadeia da Polimerase , Vírus da Febre Amarela/classificaçãoRESUMO
Filoviruses, amongst the most lethal of primate pathogens, have only been reported as natural infections in sub-Saharan Africa and the Philippines. Infections of bats with the ebolaviruses and marburgviruses do not appear to be associated with disease. Here we report identification in dead insectivorous bats of a genetically distinct filovirus, provisionally named Lloviu virus, after the site of detection, Cueva del Lloviu, in Spain.
Assuntos
Quirópteros/virologia , Reservatórios de Doenças , Ebolavirus/isolamento & purificação , Doença pelo Vírus Ebola/veterinária , Animais , Sequência de Bases , DNA Viral/análise , Surtos de Doenças , Ebolavirus/genética , Genoma , Doença pelo Vírus Ebola/patologia , Doença pelo Vírus Ebola/virologia , Pulmão/patologia , Pulmão/virologia , Dados de Sequência Molecular , Filogenia , Espanha , Baço/patologia , Baço/virologiaRESUMO
BACKGROUND: Farmington virus (FARV) is a rhabdovirus that was isolated from a wild bird during an outbreak of epizootic eastern equine encephalitis on a pheasant farm in Connecticut, USA. FINDINGS: Analysis of the nearly complete genome sequence of the prototype CT AN 114 strain indicates that it encodes the five canonical rhabdovirus structural proteins (N, P, M, G and L) with alternative ORFs (> 180 nt) in the N and G genes. Phenotypic and genetic characterization of FARV has confirmed that it is a novel rhabdovirus and probably represents a new species within the family Rhabdoviridae. CONCLUSIONS: In sum, our analysis indicates that FARV represents a new species within the family Rhabdoviridae.
Assuntos
Doenças das Aves/virologia , Infecções por Rhabdoviridae/veterinária , Rhabdoviridae/classificação , Rhabdoviridae/isolamento & purificação , Animais , Aves , Chlorocebus aethiops , Análise por Conglomerados , Connecticut , Ordem dos Genes , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Rhabdoviridae/genética , Infecções por Rhabdoviridae/virologia , Análise de Sequência de DNA , Células Vero , Proteínas Virais/genéticaRESUMO
Lymphocytic choriomeningitis virus (LCMV) was detected in 2 patients with acute meningitis in southern Spain within a 3-year period. Although the prevalence of LCMV infection was low (2 [1.3%] of 159 meningitis patients), it represents 2.9% of all pathogens detected. LCMV is a noteworthy agent of neurologic illness in immunocompetent persons.
Assuntos
Coriomeningite Linfocítica/diagnóstico , Coriomeningite Linfocítica/virologia , Vírus da Coriomeningite Linfocítica/isolamento & purificação , Adulto , Animais , Anticorpos Antivirais/sangue , Anticorpos Antivirais/líquido cefalorraquidiano , Anticorpos Antivirais/imunologia , Linhagem Celular , Chlorocebus aethiops , Feminino , Humanos , Vírus da Coriomeningite Linfocítica/classificação , Vírus da Coriomeningite Linfocítica/imunologia , Masculino , Dados de Sequência Molecular , Filogenia , Prevalência , RNA Viral/líquido cefalorraquidiano , RNA Viral/química , Espanha , Adulto JovemRESUMO
Jos virus (JOSV), originally isolated in Jos, Nigeria in 1967, has remained unclassified despite cultivation in tissue culture, development of animal models of infection and implementation of seroprevalence surveys for infection. Here, we report genetic, ultrastructural and serological evidence that JOSV is an orthomyxovirus distinct from but phylogenetically related to viruses of the genus Thogotovirus.
Assuntos
Antígenos Virais/imunologia , Genoma Viral , Thogotovirus/genética , Thogotovirus/imunologia , Proteínas Virais/imunologia , Animais , Análise por Conglomerados , Camundongos , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Nigéria , Filogenia , RNA Viral/genética , Análise de Sequência de DNA , Thogotovirus/classificação , Thogotovirus/ultraestrutura , Vírion/ultraestruturaRESUMO
The genus Phlebovirus of the family Bunyaviridae consists of approximately 70 named viruses, currently assigned to nine serocomplexes (species) based on antigenic similarities. Sixteen other named viruses that show little serologic relationship to the nine recognized groups are also classified as tentative species in the genus. In an effort to develop a more precise classification system for phleboviruses, we are attempting to sequence most of the named viruses in the genus with the goal of clarifying their phylogenetic relationships. In this report, we describe the serologic and phylogenetic relationships of 13 viruses that were found to be members of the Candiru serocomplex; 6 of them cause disease in humans. Analysis of full genome sequences revealed branching inconsistencies that suggest five reassortment events, all involving the M segment, and thus appear to be natural reassortants. This high rate of reassortment illustrates the inaccuracy of a classification system based solely on antigenic relationships.
Assuntos
Variação Genética , Phlebovirus/classificação , Phlebovirus/isolamento & purificação , RNA Viral/genética , América , Análise por Conglomerados , Genoma Viral , Humanos , Dados de Sequência Molecular , Phlebovirus/genética , Filogenia , Vírus Reordenados/genética , Análise de Sequência de DNA , Sorotipagem , Clima TropicalRESUMO
The genetic relatedness of mountain gorillas and humans has led to concerns about interspecies transmission of infectious agents. Human-to-gorilla transmission may explain human metapneumovirus in 2 wild mountain gorillas that died during a respiratory disease outbreak in Rwanda in 2009. Surveillance is needed to ensure survival of these critically endangered animals.
Assuntos
Doenças dos Símios Antropoides/epidemiologia , Gorilla gorilla/virologia , Metapneumovirus/fisiologia , Infecções por Paramyxoviridae/veterinária , Animais , Doenças dos Símios Antropoides/mortalidade , Doenças dos Símios Antropoides/transmissão , Teorema de Bayes , Feminino , Humanos , Masculino , Metapneumovirus/genética , Dados de Sequência Molecular , Infecções por Paramyxoviridae/epidemiologia , Infecções por Paramyxoviridae/mortalidade , Infecções por Paramyxoviridae/transmissão , RNA Viral/genética , Ruanda/epidemiologia , Análise de SequênciaRESUMO
Genomic and antigenic characterization of Aguacate virus, a tentative species of the genus Phlebovirus, and three other unclassified viruses, Armero virus, Durania virus and Ixcanal virus, demonstrate a close relationship to one another. They are distinct from the other nine recognized species within the genus Phlebovirus. We propose to designate them as a new (tenth) serogroup or species (Aguacate virus) within the genus. The four viruses were all isolated from phlebotomine sandflies (Lutzomyia sp.) collected in Central and South America. Aguacate virus appears to be a natural reassortant and serves as one more example of the high frequency of reassortment in this genus.
Assuntos
Antígenos Virais/imunologia , Phlebovirus/classificação , Phlebovirus/isolamento & purificação , Psychodidae/virologia , Animais , Antígenos Virais/genética , Dados de Sequência Molecular , Phlebovirus/genética , Phlebovirus/imunologia , Filogenia , Vírus Reordenados/classificação , Vírus Reordenados/genética , Vírus Reordenados/imunologia , Vírus Reordenados/isolamento & purificação , Proteínas Virais/genética , Proteínas Virais/imunologiaRESUMO
An aborted mid-gestational male Steller sea lion fetus with an attached placenta was recovered on the floor of an open floating capture trap located off Norris Rock near Denman Island, British Columbia. Viral culture of the placenta demonstrated cytopathic effect. Although no specific signal was obtained in microarray experiments using RNA obtained from viral culture, elution and sequence analysis revealed the presence of a reovirus. Complete genome pyrosequencing led to the identification of an orthoreovirus that we have tentatively named Steller sea lion reovirus (SSRV). Phylogenetic analysis revealed similarities between SSRV and orthoreoviruses of birds, bats and other mammals that suggests potential for interspecies transmission.
Assuntos
Feto Abortado/virologia , Genoma Viral , Orthoreovirus/isolamento & purificação , RNA Viral/genética , Leões-Marinhos/virologia , Animais , Colúmbia Britânica , Análise por Conglomerados , Feminino , Masculino , Dados de Sequência Molecular , Filogenia , Placenta/virologia , Gravidez , Análise de Sequência de DNARESUMO
Leanyer virus (LEAV), currently classified as a member of the genus Orthobunyavirus, in the family Bunyaviridae, was originally isolated from a pool of Anopheles meraukensis mosquitoes, collected at Leanyer, Northern Territory, Australia in 1974. When it failed to react in serological tests with antisera from other known viruses, full-length genomic sequencing was pursued to determine the relationship of LEAV to other orthobunyavirus species. Genetic and serological characterization confirmed its antigenic distance from other orthobunyaviruses, including to its closest genetic neighbours, the Simbu group viruses, suggesting that it may represent a new antigenic complex.
Assuntos
Anopheles/virologia , Orthobunyavirus/classificação , Orthobunyavirus/genética , Filogenia , Animais , Austrália , Genoma Viral , Genômica , Dados de Sequência Molecular , Orthobunyavirus/isolamento & purificaçãoRESUMO
Orbiviruses infect a wide range of hosts, including humans. The ability to detect them has been hampered by their diversity. Here we present a simple consensus reverse transcription (RT)-PCR method targeting the polymerase gene for orbivirus recognition and characterization. Phylogenetic assignment is achieved by automated Web-based sequence analysis of amplification products.