RESUMO
We retrospectively analyzed the findings of a prospective cohort of 75 children referred for thyroid nodules between 2008 and 2013. Prevalence of papillary differentiated thyroid carcinoma was 18.7%. Thyrotropin >2.5 mIU/L, multinodular goiter, solid nodules, irregular margins, and pathologic lymphadenopathies were identified as independent predictors of malignancy.
Assuntos
Neoplasias da Glândula Tireoide/diagnóstico , Nódulo da Glândula Tireoide/patologia , Adenoma/diagnóstico , Adolescente , Biópsia por Agulha Fina , Carcinoma Papilar/diagnóstico , Criança , Feminino , Seguimentos , Bócio Nodular/patologia , Humanos , Doenças Linfáticas/epidemiologia , Masculino , Valor Preditivo dos Testes , Prevalência , Estudos Prospectivos , Estudos Retrospectivos , Sensibilidade e Especificidade , Tireotropina/sangue , Adulto JovemRESUMO
CONTEXT: Pheochromocytomas and paragangliomas (pheo/pgl) are neuroendocrine tumours derived from chromaffin cells. Although mostly benign, up to 26% of pheo/pgl will undergo malignant transformation. Reliable histological signs to differentiate benign pheo/pgl from malignant tumours are currently lacking. Increased IGF-1R expression has been shown during progression to metastatic phenotypes of several types of cancer. OBJECTIVE: To analyse the distribution and expression of the IGF-1R in pheo/pgl of different genetic origin and degree of malignancy. MEASUREMENTS: We studied the expression of the IGF-1R protein by immunohistochemistry, in 40 primary tumours from patients with pheo/pgl from different genetic aetiology (11 of 29 metastatic/nonmetastatic diseases). RESULTS: We found a strong association between increased expression of IGF-1R and malignant behaviour regardless of the age at diagnosis and the genetic aetiology. IGF-1R labelling was mostly weak in primary tumours from patients with nonmetastatic pheo/pgl. Conversely, intense IGF-1R labelling was predominant in cases of pheo/pgl with confirmed metastatic disease. The risk of metastases was 11·7 times higher if tumour IGF-1R labelling was intense independently of age at diagnosis. The probability of remaining free of metastases was higher in patients with pheo/pgl scored weak for IGF-1R at 60 months and more than twofold higher at 120 months of follow-up than in patients with intense IGF-1R labelling in their primary tumours. CONCLUSIONS: Our results strongly suggest that IGF-1R is associated with malignancy in familial pheo/pgl and that IGF-1R expression in the primary tumour might be a useful tool to detect those patients harbouring pheo/pgl who have an increased risk of metastasis.
Assuntos
Paraganglioma/metabolismo , Paraganglioma/patologia , Feocromocitoma/metabolismo , Feocromocitoma/patologia , Receptor IGF Tipo 1/metabolismo , Adolescente , Neoplasias das Glândulas Suprarrenais/metabolismo , Neoplasias das Glândulas Suprarrenais/patologia , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
Gliomas are the most frequent solid tumors in children. Among these, high-grade gliomas are less common in children than in adults, though they are similar in their aggressive clinical behavior. In adults, glioblastoma is the most lethal tumor of the central nervous system. Insulin-like growth factor 1 receptor (IGF1R) plays an important role in cancer biology, and its nuclear localization has been described as an adverse prognostic factor in different tumors. Previously, we have demonstrated that, in pediatric gliomas, IGF1R nuclear localization is significantly associated with high-grade tumors, worst clinical outcome, and increased risk of death. Herein we explore the role of IGF1R intracellular localization by comparing two glioblastoma cell lines that differ only in their IGF1R capacity to translocate to the nucleus. In vitro, IGF1R nuclear localization enhances glioblastoma cell motility and metabolism without affecting their proliferation. In vivo, IGF1R has the capacity to translocate to the nucleus and allows not only a higher proliferation rate and the earlier development of tumors but also renders the cells sensitive to OSI906 therapy. With this work, we provide evidence supporting the implications of the presence of IGF1R in the nucleus of glioma cells and a potential therapeutic opportunity for patients harboring gliomas with IGF1R nuclear localization.
Assuntos
Glioblastoma , Glioma , Adulto , Carcinogênese/metabolismo , Núcleo Celular/metabolismo , Criança , Glioblastoma/metabolismo , Glioma/metabolismo , Humanos , Receptores de Somatomedina/metabolismoAssuntos
Duplicação Gênica/genética , Transtornos Ovotesticulares do Desenvolvimento Sexual/genética , Fatores de Transcrição SOXB1/genética , Proteína da Região Y Determinante do Sexo , Transtornos Testiculares 46, XX do Desenvolvimento Sexual/sangue , Transtornos Testiculares 46, XX do Desenvolvimento Sexual/genética , Transtornos Testiculares 46, XX do Desenvolvimento Sexual/patologia , Pré-Escolar , Criptorquidismo/genética , Criptorquidismo/patologia , Humanos , Hipospadia/genética , Masculino , Transtornos Ovotesticulares do Desenvolvimento Sexual/sangue , Transtornos Ovotesticulares do Desenvolvimento Sexual/patologiaRESUMO
Transgenic mice bearing a construct in which the expression of the SV40 oncogene is directed by the AMH promoter (AT mice) develop testicular tumours in adult life. We aimed to study early steps of tumour development and characterize tumours at different ages by histological, morphometric, and immunohistochemical techniques. One- to 3-month-old AT mice depicted multifocal Leydig cell hyperplasia. The testicular volume occupied by interstitial tissue was significantly higher in 3-month-old AT mice in comparison with littermate controls. Between 5 1/2 and 7 months, microscopic interstitial tumours developed that progressively evolved to form large confluent areas of high mitotic index in 7- to 14-month-old AT mice. Tumour cells had the characteristics and histoarchitecture of Leydig cells, or formed solid cord-like structures reminiscent of those seen in Sertoli cell tumours. Hyperplastic areas and tumours diffusely expressed 3beta-hydroxysteroid dehydrogenase (3beta-HSD) in Leydig cell areas. AMH expression was negative in Leydig cell conglomerates and tumours and variable in cord-like tumours. The SV40 T antigen and markers of cell proliferation (PCNA) were intensely positive in hyperplastic cells and tumours. Control mice of similar ages showed neither hyperplasia nor tumours, and SV40 T expression was always negative. In conclusion, transgenic mice develop large testicular tumours that are preceded by interstitial hyperplasia and microtumours. The histological and immunohistochemical phenotype of tumours (Leydig and Sertoli cell differentiation, positive 3beta-HSD, and variable AMH) suggests a mixed differentiation of somatic cells of the specialized gonadal stroma. The finding that an oncogene directed by a promoter specifically active in fetal Sertoli cells has given rise to testicular tumours of mixed differentiation is compatible with a common origin of Leydig and Sertoli cells from the specific stroma of the gonadal ridge, as supported by double labelling experiments in fetal mice showing co-localization of the transgene with Sertoli and Leydig cell markers.
Assuntos
Tumor de Células de Leydig/patologia , Neoplasias Testiculares/patologia , Animais , Antígenos Transformantes de Poliomavirus/metabolismo , Diferenciação Celular , Transformação Celular Neoplásica/metabolismo , Transformação Celular Neoplásica/patologia , Hiperplasia/patologia , Tumor de Células de Leydig/metabolismo , Células Intersticiais do Testículo/patologia , Masculino , Camundongos , Camundongos Transgênicos , Células de Sertoli/patologia , Neoplasias Testiculares/metabolismoRESUMO
UNLABELLED: To review our Pediatric Endocrinology Division's experience with differentiated thyroid carcinoma (DTC) we analyzed retrospectively the records of patients with DTC that had been seen between June 1988 and June 2008. RESULTS: Forty-five patients (median age 13.7 years, 36 female) were diagnosed (papillary: 40, follicular: 5) with DTC presenting as a solitary nodule (n: 25), thyroid nodule with cervical adenopathy (n: 9) and multinodular goiter (n: 11). All underwent total thyroidectomy with resection of suspicious cervical lymph nodes (CLN). DTC was multicentric in 59% and revealed extrathyroidal extension in 44%. Initially, 44% had CLN metastases and 24% distant metastases. All patients underwent thyroid remnant ablation with 131I and suppressive treatment. Median follow-up was 5.1 years with a disease-free survival rate at 5 years of follow-up of 75%. Eleven percent presented recurrences. CONCLUSION: Pediatric DTC has an aggressive behavior at presentation. Higher preoperative TSH levels were significantly associated with a more advanced disease at diagnosis. CLT was present concomitantly in a quarter of the patients and further studies are needed to establish differences in these patients' outcome. Diagnostic approach, total thyroidectomy, 131I treatment and thyrotropin suppression allowed a good progression-free survival rate.
Assuntos
Adenocarcinoma Folicular/patologia , Carcinoma Papilar/patologia , Neoplasias da Glândula Tireoide/patologia , Tireoidectomia , Adenocarcinoma Folicular/mortalidade , Adenocarcinoma Folicular/radioterapia , Adenocarcinoma Folicular/cirurgia , Adolescente , Carcinoma Papilar/mortalidade , Carcinoma Papilar/radioterapia , Carcinoma Papilar/cirurgia , Diferenciação Celular , Criança , Intervalo Livre de Doença , Feminino , Seguimentos , Bócio Nodular/patologia , Bócio Nodular/radioterapia , Bócio Nodular/cirurgia , Humanos , Radioisótopos do Iodo/uso terapêutico , Estimativa de Kaplan-Meier , Masculino , Recidiva Local de Neoplasia/diagnóstico , Recidiva Local de Neoplasia/mortalidade , Estudos Retrospectivos , Neoplasias da Glândula Tireoide/mortalidade , Neoplasias da Glândula Tireoide/radioterapia , Neoplasias da Glândula Tireoide/cirurgia , Resultado do Tratamento , Adulto JovemRESUMO
The importance of cytochrome P450 (CYP)-derived arachidonic acid (AA) metabolites, 20-hydroxyeicosatetraenoic acid (20-HETE) and epoxyeicosatrienoic acids (EETs) as tumor growth promotors has already been described in several cancer types. The aim of this study was to evaluate the role of these compounds in the biology of pheochromocytoma/paraganglioma. These tumors originate from chromaffin cells derived from adrenal medulla (pheochromocytomas) or extra-adrenal autonomic paraganglia (paragangliomas), and they represent the most common hereditary endocrine neoplasia. According to mutations in the driver genes, these tumors are divided in two clusters: pseudo-hypoxic and kinase-signaling EETs, but not 20-HETE, exhibited a potent ability to sustain growth in a murine pheochromocytoma cell line (MPC) in vitro, EETs promoted an increase in cell proliferation and a decrease in cell apoptosis. In a mouse model of pheochromocytoma, the inhibition of CYP-mediated AA metabolism using 1-aminobenzotriazol resulted in slower tumor growth, a decreased vascularization, and a lower final volume. Also, the expression of AA-metabolizing CYP monooxygenases was detected in tumor samples from human origin, being their apparent abundance and the production of both metabolites higher in tumors from the kinase-signaling cluster. This is the first evidence of the importance of CYP- derived AA metabolites in the biology and development of pheochromocytoma/paraganglioma tumors.
Assuntos
Ácido 8,11,14-Eicosatrienoico/análogos & derivados , Neoplasias das Glândulas Suprarrenais/induzido quimicamente , Sistema Enzimático do Citocromo P-450/metabolismo , Ácidos Hidroxieicosatetraenoicos/farmacologia , Feocromocitoma/induzido quimicamente , Ácido 8,11,14-Eicosatrienoico/farmacologia , Adolescente , Neoplasias das Glândulas Suprarrenais/patologia , Adulto , Animais , Linhagem Celular Tumoral , Criança , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Neovascularização Patológica , Feocromocitoma/patologia , Adulto JovemRESUMO
Anti-Müllerian hormone (AMH) is secreted by Sertoli cells of the testes from early fetal life until puberty, when it is downregulated by androgens. In conditions like complete androgen insensitivity syndrome (CAIS), AMH downregulation does not occur and AMH increases at puberty, due in part to follicle-stimulating hormone (FSH) effect. However, other conditions like Peutz-Jeghers syndrome (PJS), characterised by low FSH, also have increased AMH. Because both CAIS and PJS may present as hyperoestrogenic states, we tested the hypothesis that oestradiol (E2) upregulates AMH expression in peripubertal Sertoli cells and explored the molecular mechanisms potentially involved. The results showed that E2 is capable of inducing an upregulation of endogenous AMH and of the AMH promoter activity in the prepubertal Sertoli cell line SMAT1, signalling through ERα binding to a specific ERE sequence present on the hAMH promoter. A modest action was also mediated through the membrane oestrogen receptor GPER. Additionally, the existence of ERα expression in Sertoli cells in patients with CAIS was confirmed by immunohistochemistry. The evidence presented here provides biological plausibility to the hypothesis that testicular AMH production increases in clinical conditions in response to elevated oestrogen levels.
Assuntos
Síndrome de Resistência a Andrógenos/metabolismo , Hormônio Antimülleriano/metabolismo , Receptor alfa de Estrogênio/biossíntese , Proteínas de Neoplasias/biossíntese , Síndrome de Peutz-Jeghers/metabolismo , Elementos de Resposta , Células de Sertoli/metabolismo , Síndrome de Resistência a Andrógenos/patologia , Animais , Linhagem Celular , Criança , Pré-Escolar , Estradiol/metabolismo , Feminino , Humanos , Masculino , Camundongos , Síndrome de Peutz-Jeghers/patologia , Células de Sertoli/patologiaRESUMO
Insulin-like growth factor 1 (IGF1) has a critical role in maintaining tumor phenotype and survival of already transformed murine pheochromocytoma (pheo) cells (MPC4/30) and it is required for the initial establishment of these tumors. However, the role of local IGF1/IGF1R system in tumor microenvironment has not been fully understood. In vivo, by subcutaneous injection of pheo cells in heterozygous IGF1R knockout mice (L/n), we found that the time of noticeable tumor appearance was delayed, and incidence was decreased in L/n group compared to control (L/L) mice. Once established, tumor proliferation, vascularization or growth rate did not differ between groups. In vitro, fibroblast from L/n and L/L mice were cultured to generate conditioned media (CM) and differential matrixes on which pheo cells were seeded. Proliferation rate was higher when pheo cells were cultured with CM, or in differential matrix generated by L/L murine fibroblasts. A diminished fibronectin (FN) expression and secretion from L/n fibroblast was associated with decreased expression of integrin subunits in tumor cells. Also, soluble factors as IGF1 and insulin-like growth factor binding protein 2 (IGFBP2) were reduced. Our data suggest that IGF1 signaling through IGF1R may contribute to tumor cells anchorage and survival by interaction with both matrix and soluble factors produced by tumor microenvironment fibroblasts.
Assuntos
Neoplasias das Glândulas Suprarrenais/fisiopatologia , Proliferação de Células , Fibroblastos/metabolismo , Haploinsuficiência , Feocromocitoma/fisiopatologia , Receptor IGF Tipo 1/genética , Microambiente Tumoral , Neoplasias das Glândulas Suprarrenais/genética , Neoplasias das Glândulas Suprarrenais/metabolismo , Animais , Fibronectinas/genética , Regulação Neoplásica da Expressão Gênica , Masculino , Camundongos , Neovascularização Patológica , Feocromocitoma/genética , Feocromocitoma/metabolismoRESUMO
Sertoli cells are necessary to provide adequate levels of lactate for germ cell development. Lactate production is hormonally regulated by follicle-stimulating hormone (FSH) and by a large set of intratesticular regulators such as interleukin-1 beta (IL1 beta) and basic fibroblast growth factor (bFGF). Little is known regarding the critical step in the production of this metabolite, viz., the entrance of glucose into the cell as mediated by GLUTs. The aim of the present study was to investigate the expression of the glucose transporters GLUT1 and GLUT3 and its possible regulation by FSH, IL1 beta, and bFGF in Sertoli cells at two different time-points in sexual development. Sertoli cells retaining the ability to undergo mitosis (obtained from 8-day-old rats) and in the process of terminal differentiation (obtained from 20-day-old rats) were examined. Testicular tissue sections and Sertoli cell monolayers obtained from 8- and 20-day-old rats showed positive immunostaining for GLUT1 and GLUT3 proteins. GLUT1 and GLUT3 mRNA levels were detected at the two ages analyzed. Treatment of Sertoli cells obtained from 8- and 20-day-old rats with FSH, IL1 beta, and bFGF for various periods of time (12, 24, and 48 h) increased GLUT1 without changing GLUT3 mRNA levels. Our results thus show that Sertoli cells express GLUT1 and GLUT3 throughout pubertal development, and that, in Sertoli cells, only GLUT1 is regulated by hormones during pubertal development. Hormonal regulation of GLUT1 expression and consequently glucose uptake and lactate production may be a key molecular event in the regulation of spermatogenesis by hormones.
Assuntos
Transportador de Glucose Tipo 1/biossíntese , Transportador de Glucose Tipo 3/biossíntese , Células de Sertoli/metabolismo , Animais , Fator 2 de Crescimento de Fibroblastos/farmacologia , Fator 2 de Crescimento de Fibroblastos/fisiologia , Hormônio Foliculoestimulante/farmacologia , Hormônio Foliculoestimulante/fisiologia , Transportador de Glucose Tipo 1/efeitos dos fármacos , Transportador de Glucose Tipo 3/efeitos dos fármacos , Interleucina-1beta/farmacologia , Interleucina-1beta/fisiologia , Ácido Láctico/biossíntese , Masculino , Ratos , Ratos Sprague-Dawley , Células de Sertoli/citologia , Células de Sertoli/efeitos dos fármacosRESUMO
Nuclear localization of insulin-like growth factor receptor type 1 (IGF-1R) has been described as adverse prognostic factor in some cancers. We studied the expression and localization of IGF-1R in paediatric patients with gliomas, as well as its association with World Health Organization (WHO) grading and survival. We conducted a single cohort, prospective study of paediatric patients with gliomas. Samples were taken at the time of the initial surgery; IGF-1R expression and localization were characterized by immunohistochemistry (IHC), subcellular fractionation and western blotting. Tumours (47/53) showed positive staining for IGF-1R by IHC. IGF-1R nuclear labelling was observed in 10/47 cases. IGF-1R staining was mostly non-nuclear in low-grade tumours, while IGF-1R nuclear labelling was predominant in high-grade gliomas (p = 0.0001). Survival was significantly longer in patients with gliomas having non-nuclear IGF-1R localization than in patients with nuclear IGF-1R tumours (p = 0.016). In gliomas, IGF-1R nuclear localization was significantly associated with both high-grade tumours and increased risk of death. Based on a prospective design, we provide evidence of a potential usefulness of intracellular localization of IGF-1R as prognostic factor in paediatric patients with gliomas.
Assuntos
Biomarcadores Tumorais/metabolismo , Neoplasias Encefálicas/metabolismo , Núcleo Celular/metabolismo , Glioma/metabolismo , Receptor IGF Tipo 1/metabolismo , Transporte Ativo do Núcleo Celular , Adolescente , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/mortalidade , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Glioma/diagnóstico , Glioma/mortalidade , Humanos , Imuno-Histoquímica , Lactente , Masculino , Estadiamento de Neoplasias , Prognóstico , Estudos Prospectivos , Receptor IGF Tipo 1/genética , Análise de Sobrevida , Organização Mundial da SaúdeRESUMO
Testicular dysgenesis derives from abnormal gonadal development caused by chromosome aberrations/mosaicisms or mutations/deletions in SRY or other genes responsible for testicular differentiation. Dysgenetic male pseudohaermaphroditism has bilateral dysgenetic testes characterized by a cortical network of anastomosing seminiferous cords that penetrate a thin albuginea. In asymmetric gonadal differentiation (or Mixed Gonadal Dysgenesis) a dysgenetic testis associates with a streak gonad with primitive sex cords embedded in an ovarian-like stroma. Uni- or bilateral ovotestes identify true haermaphroditism. Fluorescent in situ hybridisation studies demonstrate that the sex chromosomes of mosaic patients do not distribute homogeneously in asymmetric gonads. 45,X lines predominate over 46,XY in streak gonads, while the relationship between these two is more equivalent in dysgenetic testes, suggesting that testicular or streak differentiation is related to the balance between X0 and XY lines. Testicular dys-genesis is more severe when there is a frank predominance of X0 or XX cells. Higher percentages of XY cells coincide with lesser degrees of dysgenesis. DNA densitometry indicate a higher incidence of neoplastic transformation than previously anticipated. Various specimens showed clear aneuploid histograms but no clear indication of a cytological CIS phenotype. There was a wide cytological variation in aneuploid germ cells, ranging from normally looking big infantile spermatogonia to gonocyte/CIS cells. Aneuploidy probably precedes the full expression of the CIS phenotype. In case of doubt we recommend DNA densitometry to either confirm or discard their neoplastic nature. The earliest recognizable change in germ cell tumorigenesis is probably the polyploidisation of fetal germ cells, followed by the expression of the CIS phenotype in isolated germ cells scattered along infantile seminiferous tubules that later proliferate to give an adult type CIS pattern.
Assuntos
Disgenesia Gonadal/patologia , Lesões Pré-Cancerosas/patologia , Neoplasias Testiculares/patologia , Testículo/anormalidades , Criança , Cromossomos Humanos X/genética , Cromossomos Humanos Y/genética , DNA/análise , Densitometria , Transtornos do Desenvolvimento Sexual/genética , Disgenesia Gonadal/genética , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Masculino , Fenótipo , Lesões Pré-Cancerosas/genética , Neoplasias Testiculares/genética , Testículo/patologiaRESUMO
Thyroidectomy augments the natriuretic response to volume expansion; however, the mechanism remains unknown. This study assessed the role of 20-hydroxyeicosatetraenoic acid (20-HETE) in the natriuretic response to an acute volume expansion in hypothyroid rats. Urine flow (1.9-fold), sodium excretion (2.4-fold), fractional sodium excretion (3.8-fold), and distal delivery of sodium (4.1-fold) increased to a greater extent in thyroidectomized rats (TX) than in sham-operated controls (SHAM) following i.v. infusion of isotonic saline (5% body weight) over 60 min. This was associated with inhibition of both proximal and distal tubular reabsorption of sodium. Administration of two mechanistic and chemical dissimilar inhibitors of the synthesis of 20-HETE, 1-aminobenzotriazole (ABT), and N-hydroxy-N'-(-4-butyl-2-methylphenyl)formamidine (HET0016) decreased the natriuretic response in TX rats. Glomerular filtration rate was lower in TX than in SHAM rats and was not altered by the CYP4A inhibitors. The expression, intrarenal distribution, and the formation of 20-HETE and expoxygenase metabolites of arachidonic acid were similar in the cortex and medulla of SHAM and TX rats. These results suggest that CYP4A-derived metabolites of arachidonic acid play an important role in the enhanced natriuretic response to volume expansion in hypothyroid rats even though TX did not alter the expression or activity of these enzymes.
RESUMO
Introducción: La caracterización de los nódulos tiroideos en pediatría no ha sido aún bien comunicada. El riesgo de malignidad es mayor que en adultos por lo que requieren una evaluación exhaustiva. Objetivo: caracterizar una cohorte de pacientes pediátricos con nódulos tiroideos e identificar predictores de malignidad. Pacientes y métodos: se analizaron los hallazgos demográficos, clínicos, bioquímicos, ecográficos y citológicos de una cohorte prospectiva de 106 pacientes <19 años que consultó por nódulo tiroideo a nuestro centro entre 2008-2017. 89 pacientes alcanzaron el diagnóstico de nódulo benigno o maligno por cirugía y 17 luego de un seguimiento clínico mínimo de 2 años. Retrospectivamente se analizaron las diferencias entre los nódulos benignos y malignos. Resultados: la edad mediana fue 13,9 años, con franco predominio de mujeres puberales. 88% presentó una función tiroidea normal. 88/106 nódulos fueron benignos. El carcinoma papilar de tiroides (CPT) fue la única lesión maligna hallada (17%). El análisis estadístico mostró una asociación significativa de CPT con TSH >2,5mU/l, nódulo sólido, márgenes irregulares, microcalcificaciones y adenopatías cervicales patológicas. Un resultado citológico Bethesda V/VI tuvo un valor predictivo (VP) positivo de 87,5% y VP negativo de 96,4%. Conclusiones: El nódulo tiroideo en pediatría es más frecuentemente maligno. La evaluación sistemática permitió identificar ciertos hallazgos clínicos, bioquímicos, ecográficos y citológicos predictores de malignidad que deben ser considerados al decidir el enfoque diagnóstico
Introduction: Published data on pediatric thyroid nodule´s characterization is scarce. With higher risk of malignancy than in adults, they require an exhaustive diagnostic work-up. Objective: To characterize a pediatric cohort with thyroid nodules to identify predictors of malignancy. Patients and methods: Demographic, clinical, biochemical, ultrasonographical and cytological data were analyzed prospectively in 106 patients <19 years that consulted with a thyroid nodule to our center (2008-2017). 89 patients reached final diagnosis (benign or malignant nodule) after surgery and 17 after a minimum follow-up of 2years. Differences between benign and malignant nodules were analyzed retrospectively. Results: median age was 13.9 years, with predominant pubertal females. 88% was euthyroid. 88/106 nodules were benign. Thyroid papillary carcinoma (TPC) was the only malignancy found (17%). Statistical analysis showed significant association of TPC with TSH levels >2.5mU/l, solid nodules, irregular margins, microcalcifications and pathologic adenopathies. Cytological results Bethesda V/VI showed positive and negative predictive values of 87.5% and 96.4% respectively. Conclusions: Pediatric thyroid nodules are more frequently malignant. The systematic evaluation of our cohort allowed the identification of clinical, biochemical ultrasonographical and cytological predictors of malignancy that have to be considered when deciding the diagnostic approach
Assuntos
Humanos , Nódulo da Glândula Tireoide , Pediatria , Criança , Adolescente , NeoplasiasRESUMO
El Síndrome de Turner es un desorden cromosómico causado por haploinsuficiencia completa o parcial de uno de los cromosomas sexuales. Incidencia 1: 2500 recién nacidas vivas. Clínicamente las pacientes presentan talla baja, un espectro amplio de anomalías somáticas y disgenesia gonadal. Desde el año 1968 hasta el presente se estudiaron clínica y citogenéticamente 630 niñas con fenotipo de Turner, sin ambigüedad genital y con cariotipos anormales, quienes consultaron en la División de Endocrinología del Hospital de Niños "Ricardo Gutiérrez". Se realizó cariotipo en sangre, al inicio con metodología estándar, luego con diferentes bandeos convencionales y de alta resolución. En casos especiales se aplicó la técnica FISH y el análisis molecular de los cromosomas X e Y. El número de metafases analizadas también varió con el tiempo, permitiendo evidenciar más de una línea celular. En casos de alta sospecha clínica, la lectura de 100 metafases permitió poner en evidencia mosaicismos bajos conteniendo la línea 45,X. En nuestra serie la monosomía de cromosoma sexual 45, X fue la más frecuente siguiendo los mosaicos numéricos y estructurales de uno de los cromosomas sexuales. Los diferentes hallazgos cromosómicos nos han permitido establecer una correlación fenotipo-cariotipo en regiones específicas de los cromosomas sexuales
Turner Syndrome is a common chromosomal disorder caused by total or partial haploinsufficiency of one of the sex chromosomes. Incidence: 1: 2500. Clinically is characterized by short stature, several typical somatic features, and gonadal dysgenesis. This is a retrospective study involving 630 girls with Turner phenotype and abnormal karyotype, evaluated at the Endocrinology Division of Children´s Hospital "Ricardo Gutiérrez" between 1968 and 2018. The karyotype was done in leucocytes from peripheral blood and the metaphases were analyzed at the beginning with standard methodology and then with different banding techniques, standard and high resolution. In special cases, the FISH technique and the molecular analysis of the X and Y chromosomes were applied. The number of metaphases analyzed also changed with time, allowing the finding of more than one cellular line. In cases where the clinical suspicion was strong, the analysis of 100 metaphases allowed us to put in evidence low mosaicisms containing the 45,X line. In our study the monosomy of sexual chromosome 45, X was the most frequent following the numerical and structural mosaics of one of the sex chromosomes. The different chromosomal constitutions have contributed to establish in our patients phenotype-karyotype correlation with specific regions of the sex chromosomes
Assuntos
Humanos , Cromossomos Sexuais , Síndrome de Turner , Fenótipo , Argentina , GenótipoRESUMO
IGFs are involved in malignant transformation and growth of several tissues, including the adrenal medulla. The present study was designed to evaluate the impact of IGF-I on pheochromocytoma development. We used a murine pheochromocytoma (MPC) cell line (MPC4/30) and an animal model with a reduction of 75% in circulating IGF-I levels [liver-IGF-I-deficient (LID) mice] to perform studies in vitro and in vivo. We found that, in culture, IGF-I stimulation increases proliferation, migration, and anchorage-independent growth, whereas it inhibits apoptosis of MPC cells. When injected to control and to LID mice, MPC cells grow and form tumors with features of pheochromocytoma. Six weeks after cell inoculation, all control mice developed sc tumors. In contrast, in 73% of LID mice, tumor development was delayed to 7-12 wk, and the remaining 27% did not develop tumors up to 12 wk after inoculation. LID mice harboring MPC cells and treated with recombinant human IGF-I (LID+) developed tumors as controls. Tumors developed in control, LID, and LID+ mice had similar histology and were similarly positive for IGF-I receptor expression. The apoptotic index was higher in tumors from LID mice compared with those from control mice, whereas vascular density was decreased. In summary, our work demonstrates that IGF-I has a critical role in maintaining tumor phenotype and survival of already transformed pheochromocytoma cells and is required for the initial establishment of these tumors, providing encouragement to carry on research studies to address the IGF-I/IGF-I receptor system as a target of therapeutic strategies for pheochromocytoma treatment in the future.
Assuntos
Fator de Crescimento Insulin-Like I/farmacologia , Feocromocitoma/metabolismo , Animais , Apoptose/efeitos dos fármacos , Western Blotting , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Humanos , Imuno-Histoquímica , Imunoprecipitação , Masculino , Camundongos , Células PC12 , Ratos , Receptor IGF Tipo 1/metabolismoRESUMO
From fetal life to adulthood, the testis evolves through maturational phases showing specific morphologic and functional features in its different compartments. The seminiferous cords contain Sertoli and germ cells, surrounded by peritubular cells, and the interstitial tissue contains Leydig cells and connective tissue. Sertoli cells secrete anti-Müllerian hormone (AMH), whereas Leydig cells secrete androgens. In the fetal and early postnatal testis, Leydig cells actively secrete androgens. Sertoli cells are morphologically and functionally immature--e.g., they secrete high levels of AMH--and germ cells proliferate by mitosis but do not enter meiosis. During infancy and childhood, Leydig cells regress and testosterone secretion declines dramatically. Sertoli cells remain immature and spermatogenesis is arrested at the premeiotic stage. At puberty, Leydig cells differentiate again, and testosterone concentration increases and provokes Sertoli cell maturation--e.g., down-regulation of AMH expression--and germ cells undergo meiosis, the hallmark of adult spermatogenesis driving to sperm production. An intriguing feature of testicular development is that, although testosterone production is as active in the fetal and early postnatal periods as in puberty, Sertoli cells and spermatogenesis remain immature until pubertal onset. Here, we review the ontogeny of the androgen receptor expression in the testis and its impact on Sertoli cell maturation and the onset of pubertal spermatogenesis. We show that the absence of androgen receptor expression in Sertoli cells underlies a physiological stage of androgen insensitivity within the male gonad in the fetal and early postnatal periods.
Assuntos
Receptores Androgênicos/biossíntese , Células de Sertoli/fisiologia , Espermatogênese/fisiologia , Testículo/crescimento & desenvolvimento , Testículo/fisiologia , Adolescente , Androgênios/fisiologia , Criança , Pré-Escolar , Feto , Humanos , Lactente , Recém-Nascido , MasculinoRESUMO
McCune-Albright syndrome (MAS), usually presenting with polyostotic bone dysplasia, café-au-lait skin lesions and sexual precocity, results from a somatic activating mutation of the GNAS1 gene, which encodes the Gs-alpha protein involved in signalling of several G-protein-coupled receptors. The clinical spectrum depends on tissue distribution of mutant-bearing cells. Sexual precocity has been ascribed to the occurrence of a mutant GNAS1 allele in the gonadal anlage, from which all somatic cells of the differentiated gonads arise. In boys, precocious activation of Leydig cell androgen secretion results in pubertal spermatogenesis, leading to testicular enlargement, and in the development of secondary sex characteristics. However, sexual precocity is rare in MAS males while isolated testicular enlargement is frequently observed. We recently reported the case of a boy with macro-orchidism and signs of Sertoli cell hyperactivity but no signs of hyperandrogenism, which was unexpected since Gs-alpha is functional in both Sertoli and Leydig cells. To understand its pathophysiology, we microdissected an available testicular biopsy to separate Sertoli from Leydig cells. The R201H-GNAS1 allele was present only in Sertoli cells, resulting in isolated Sertoli cell hyperfunction, evidenced by increased AMH expression and cell hyperplasia leading to prepubertal macro-orchidism, with no signs of Leydig cell activation. The different early embryologic origin of precursors contributing to Sertoli and Leydig cell lineages may underlie the differential existence of the mutated GNAS1 gene. Lack of occurrence of the mutation in Leydig cells may explain why sexual precocity is rarely observed in boys with MAS.
Assuntos
Displasia Fibrosa Poliostótica/genética , Subunidades alfa Gs de Proteínas de Ligação ao GTP/genética , Mosaicismo , Mutação , Puberdade Precoce/genética , Testículo/metabolismo , Sequência de Bases , Linhagem Celular , Criança , Pré-Escolar , Cromograninas , Displasia Fibrosa Poliostótica/fisiopatologia , Subunidades alfa Gs de Proteínas de Ligação ao GTP/fisiologia , Humanos , Células Intersticiais do Testículo/metabolismo , Células Intersticiais do Testículo/patologia , Luciferases/genética , Luciferases/metabolismo , Masculino , Modelos Biológicos , Regiões Promotoras Genéticas , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Células de Sertoli/metabolismo , Células de Sertoli/patologia , Transdução de Sinais , Testículo/patologia , TransfecçãoRESUMO
Introducción.La pancreatitis en la infancia es considerada una enfermedad poco frecuente.Se estudiaron retrospectivamente 47 necropsias con diagnóstico histopatológico de pancreatitis con el fin de analizar las características clínicas y anatomopatológicos de esta enfermedad poco frecuente.Se estudiaron retrospectivamente 47 necropsias con diagnóstico histopatológico de pancreatitis con el fin de analizar las características clínicas y anatomopatológicas de esta enfermedad.material y métodos.Las observaciones se agruparon de acuerdo a la etiología de la pamcreatitisinfecciosa en 29 pacientes,secundaria a drogas o tóxicos en 4,post-quirúrgica o secundaria a la obstrucción del flujo biliar en 3 de casa uno y 8 necropsias con causas diversas.Se analizaron las historias clínicas y se encontró que existían síntomas sugerentes de pancreatitis en 10(21 por ciento) pacientes del total,sin que se observara ninguna relación con las etiologías.Resultados.No hubo correlación entre etiología y tipo histológico,se observaron 19 pancreatitis agudas hemorrágicas,17 agudas intersticiales,7 crónicas y 4 granulomatosas.Se consideró luego del análisis de cada necropsia que la pancreatitis contribuyó a la muerte en 35 niños(74 por ciento).Conclusiones.En esta muestra la pancreatitis frecuentemente fue la etiología infecciosa,tuvo cuadro histopatológico no específico,produjo escasos síntomasy,por la severidad del daño estuvo,probablemente relacionada con la muerte
Assuntos
Humanos , Criança , Pancreatite/fisiopatologia , Pancreatite/história , PediatriaRESUMO
Introducción.La pancreatitis en la infancia es considerada una enfermedad poco frecuente.Se estudiaron retrospectivamente 47 necropsias con diagnóstico histopatológico de pancreatitis con el fin de analizar las características clínicas y anatomopatológicos de esta enfermedad poco frecuente.Se estudiaron retrospectivamente 47 necropsias con diagnóstico histopatológico de pancreatitis con el fin de analizar las características clínicas y anatomopatológicas de esta enfermedad.material y métodos.Las observaciones se agruparon de acuerdo a la etiología de la pamcreatitisinfecciosa en 29 pacientes,secundaria a drogas o tóxicos en 4,post-quirúrgica o secundaria a la obstrucción del flujo biliar en 3 de casa uno y 8 necropsias con causas diversas.Se analizaron las historias clínicas y se encontró que existían síntomas sugerentes de pancreatitis en 10(21 por ciento) pacientes del total,sin que se observara ninguna relación con las etiologías.Resultados.No hubo correlación entre etiología y tipo histológico,se observaron 19 pancreatitis agudas hemorrágicas,17 agudas intersticiales,7 crónicas y 4 granulomatosas.Se consideró luego del análisis de cada necropsia que la pancreatitis contribuyó a la muerte en 35 niños(74 por ciento).Conclusiones.En esta muestra la pancreatitis frecuentemente fue la etiología infecciosa,tuvo cuadro histopatológico no específico,produjo escasos síntomasy,por la severidad del daño estuvo,probablemente relacionada con la muerte