RESUMO
Early and sensitive detection of tobacco mosaic virus (TMV) is of great significance for improving crop yield and protecting germplasm resources. Herein, we constructed a novel fluorescence sensor to detect TMV RNA (tRNA) through double strand specific nuclease (DSN) cycle and activator regenerative electron transfer atom transfer radical polymerization (ARGET ATRP) dual signal amplification strategy. The hairpin DNA complementarily paired with tRNA was used as a recognition unit to specifically capture tRNA. By the double-stranded DNA hydrolyzed with DSN, tRNA is released to open more hairpin DNA, and more complementary DNA (cDNA) is bound to the surface of the magnetic beads (MBs) to achieve the first amplification. After binding with the initiator, the cDNA employed ARGET ATRP to attach more fluorescent signal molecules to the surface of MBs, thus achieving the second signal amplification. Under the optimal experimental conditions, the logarithm of fluorescence intensity versus tRNA concentration showed a good linear relationship in the range of 0.01-100 pM, with a detection limit of 1.03 fM. The limit of detection (LOD) was calculated according to LOD = 3 N/S. Besides, the sensor showed good reproducibility and stability, which present provided new method for early and highly sensitive detection for plant viruses.
Assuntos
RNA Viral , Vírus do Mosaico do Tabaco , Vírus do Mosaico do Tabaco/genética , Vírus do Mosaico do Tabaco/química , RNA Viral/análise , Fluorescência , Limite de Detecção , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química , Espectrometria de FluorescênciaRESUMO
A novel aptamer-based sensor was developed using the signal amplification strategy of ring-opening metathesis polymerization (ROMP) and polyethyleneimine modified graphene oxide to achieve trace detection of carbendazim (CBZ). The dual identification of aptamer and antibody was used to avoid false positive results and improve the selectivity. Polyethyleneimine modified graphene oxide (GO-PEI), as a substrate material with excellent conductivity, was modified on the surface of a glassy carbon electrode (GCE) to increase the grafting amount of aptamer on the electrode surface. Moreover, a large number of cyclopentenyl ferrocene (CFc) was aggregated to form long polymer chains through ring-opening metathesis polymerization (ROMP), so as to significantly improve the detection sensitivity of the biosensor. The linear range of this sensor was 1 pg/mL-100 ng/mL with a detection limit as low as 7.80 fg/mL. The sensor exhibited excellent reproducibility and stability, and also achieved satisfactory results in actual sample detection. The design principle of such a sensor could provide innovative ideas for sensors in the detection of other types of targets.
Assuntos
Aptâmeros de Nucleotídeos , Benzimidazóis , Técnicas Biossensoriais , Carbamatos , Técnicas Eletroquímicas , Grafite , Limite de Detecção , Polietilenoimina , Polimerização , Grafite/química , Carbamatos/química , Carbamatos/análise , Técnicas Eletroquímicas/métodos , Técnicas Eletroquímicas/instrumentação , Polietilenoimina/química , Técnicas Biossensoriais/métodos , Benzimidazóis/química , Aptâmeros de Nucleotídeos/química , Eletrodos , Reprodutibilidade dos TestesRESUMO
A unique combination of a specific nucleic acid restriction endonuclease (REase) and atom transfer radical polymerization (ATRP) signal amplification strategy was employed for the detection of T790M mutations prevalent in the adjuvant diagnosis of lung cancer. REase selectively recognizes and cleaves T790M mutation sites on double-stranded DNA formed by hybridization of a capture sequence and a target sequence. At the same time, the ATRP strategy resulted in the massive aggregation of upconverted nanoparticles (UCNPs), which significantly improved the sensitivity of the biosensor. In addition, the UCNPs have excellent optical properties and can eliminate the interference of autofluorescence in the samples, thus further improving the detection sensitivity. The proposed upconversion fluorescent biosensor is characterized by high specificity, high sensitivity, mild reaction conditions, fast response time, and a detection limit as low as 0.14 fM. The performance of the proposed biosensor is comparable to that of clinical PCR methods when applied to clinical samples. This work presents a new perspective for assisted diagnosis in the pre-intervention stage of tumor diagnostics in the early stage of precision oncology treatments.
Assuntos
Técnicas Biossensoriais , Neoplasias Pulmonares , Humanos , Neoplasias Pulmonares/genética , Enzimas de Restrição do DNA , Receptores ErbB/genética , Polimerização , Clivagem do DNA , Limite de Detecção , Mutação , Medicina de Precisão , Inibidores de Proteínas Quinases , Técnicas Biossensoriais/métodosRESUMO
The levels of KRAS G12C point mutation is recognized to be closely related to the earlier diagnosis of non-small cell lung cancer (NSCLC). Here, based on nitrogen-doped graphene quantum dots (NGQDs) and photo-induced electron/energy transfer reversible addition-fragment chain transfer (PET-RAFT) signal amplification strategy, we fabricated a novel electrochemiluminescence (ECL) biosensor for the detection of KRAS G12C mutation for the first time. NGQDs as ECL-emitting species with cathodic ECL were prepared by a simple calcination method. Firstly, KRAS G12C mutation DNA, i. e., target DNA (tDNA), was captured by specific identification with hairpin DNA (hDNA). Then, PET-RAFT was initiated by blue light, and large numbers of monomers were successfully polymerized to form controllable polymer chains. Lastly, massive NGQDs was introduced via amidation reaction with N-(3-aminopropyl)methacrylamide hydrochloride (APMA), which significantly amplified the ECL signal intensity. Under optimal conditions, this biosensor achieved a good linear relationship between ECL intensity and logarithm of the levels of KRAS G12C mutation in the range from 10â fM to 10â nM. Moreover, this strategy exhibited high selectivity and excellent applicability for KRAS G12C mutation detection in the serum samples. Therefore, this biosensor has great potential in clinical diagnosis and practical application.
Assuntos
Técnicas Biossensoriais , Carcinoma Pulmonar de Células não Pequenas , Grafite , Neoplasias Pulmonares , Pontos Quânticos , Humanos , Proteínas Proto-Oncogênicas p21(ras)/genética , Nitrogênio , Medições Luminescentes/métodos , DNA , Técnicas Biossensoriais/métodos , Mutação , Tomografia por Emissão de PósitronsRESUMO
Exosome is an emerging tumor marker, whose concentration level can reflect the occurrence and development of tumors. The development of rapid and sensitive exosome detection platform is of great significance for early warning of cancer occurrence. Here, a strategy for electrochemical detection of A549-cell-derived exosomes was established based on DNA/ferrocene-modified single-walled carbon nanotube complex (DNA/SWCNT-Fc). DNA/SWCNT-Fc complexes function as a signal amplification platform to promote electron transfer between electrochemical signal molecules and electrodes, thereby improving sensitivity. At the same time, the exosomes can be attached to DNA/SWCNT-Fc nanocomposites via the established PO43--Ti4+-PO43- method. Moreover, the application of EGFR antibody, which can specifically capture A549 exosomes, could improve the accuracy of this sensing system. Under optimal experimental conditions, the biosensor showed good linear relationship between the peak current and the logarithm of exosomes concentration from 4.66 × 106 to 9.32 × 109 exosomes/mL with a detection limit of 9.38 × 104 exosomes/mL. Furthermore, this strategy provides high selectivity for exosomes of different cancer cells, which can be applied to the detection of exosomes in serum samples. Thus, owing to its advantages of high sensitivity and good selectivity, this method provides a diversified platform for exosomes identification and has great potential in early diagnosis and biomedical applications.
Assuntos
Exossomos , Nanotubos de Carbono , Metalocenos , DNARESUMO
An ochratoxin A (OTA) electrochemical biosensor based on a cascade signal amplification strategy with Ag nanoparticles (AgNPs) and ring opening polymerization (ROP) was constructed. The large specific surface area of AgNPs was used to increase the loading of OTA aptamer on the electrode surface, enhancing the ability to capture OTA as a way to achieve the first signal amplification. The OTA antibody modified with polyethylenimine specifically recognizes the OTA, forming an aptamer-OTA-antibody sandwich structure. The amino group on polyethylenimine initiates the ROP reaction with α-amino acid-n-carboxylic anhydride-ferrocene (NCA-Fc) as the monomer. A large number of electrochemical signal units of ferrocene are introduced into the sensing system for a second signal amplification. By amplifying the signal twice, the sensitivity of the sensor is improved. Under the optimal conditions, the detection range of the sensor is 1 pg·mL-1 ~ 1 µg·mL-1, while the detection limit is as low as 117 fg·mL-1. Moreover, the sensor has the advantages of high sensitivity, good stability and selectivity. Standard addition recovery experiment proved that the sensing system can be successfully used for the detection of OTA in four actual samples with recoveries in the range 90.0 to 113% with RSDs of 0.6 to 5.2%, providing a new idea for the pollution assessment of mycotoxins.
Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Metalocenos/química , Nanopartículas Metálicas/química , Polietilenoimina , Polimerização , Técnicas Eletroquímicas , PrataRESUMO
Herein, an electroluminescence (ECL) biosensor was constructed by combining click chemistry with activators regenerated by electron transfer-atom transfer radical polymerization (ARGET-ATRP) to sensitively assay tobacco mosaic virus (TMV) RNA for the first time. First, hairpin DNA (hDNA) was self-assembled on the gold electrode surface through Au-S bonding. The hDNA hybridized with the tDNA to form tRNA/hDNA hybrids in the presence of TMV RNA (tRNA), so that the azide group labelled at the end of the hDNA was kept away from the electrode surface. Subsequently, the initiator for the ARGET-ATRP reaction was modified on the electrode surface by chemical bonds via click chemistry. Then, N-acryloxysuccinimide (NAS)-labelled polymer chains were successfully formed on the electrode surface by ARGET-ATRP. Under the optimized conditions, a good linear relationship existed with the ECL signal and the logarithm of tRNA concentration in the range of 0.1 pM-10 nM, and the limit of detection was 2.61 fM. In addition, this strategy can identify mismatched bases and performs well in recovery assays in real samples. For its high sensitivity, selectivity, simplicity and economy, the ECL biosensor shows great potential for practical applications.
Assuntos
Técnicas Biossensoriais , Vírus do Mosaico do Tabaco , Química Click , Polimerização , RNA , Vírus do Mosaico do Tabaco/genéticaRESUMO
Alkaline phosphatase (ALP), an important hydrolase involved in dephosphorylation, is a common clinical indicator of many diseases. In the present study, we constructed a novel electrochemical sensor using amifostine as the substrate of ALP and activators regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) as a signal amplification strategy for sensitive determination of ALP activity. In particular, in the presence of ALP, the phosphate group of amifostine was hydrolyzed to form a sulfhydryl group, which could attach to a gold electrode via a sulfur-gold bond. Then, the initiator α-bromophenylacetic acid (BPAA) was linked to the hydrolysis product of amifostine through an amide bond, resulting in the production of electroactive polymer chains on the gold electrode by the monomer ferrocenylmethyl methacrylate (FMMA) via ARGET ATRP. Under optimal parameters, the electrochemical sensor demonstrated a limit of detection (LOD) of 1.71 mU mL-1 with a linear range of 5-100 mU mL-1. In addition to satisfactory selectivity, the potential application of this approach for ALP activity detection in human serum samples was demonstrated. Due to its efficiency, simplicity of operation, and cost-effectiveness, the proposed electrochemical sensor has great promise as a universal method for ALP assays and inhibitor screening.
Assuntos
Amifostina , Técnicas Biossensoriais , Fosfatase Alcalina , Técnicas Biossensoriais/métodos , DNA/química , Técnicas Eletroquímicas/métodos , Ouro/química , Humanos , Limite de DetecçãoRESUMO
The concentration level of cytokeratin fragment antigen 21-1 (CYFRA21-1) can be used as an important indicator for predicting non-small cell lung cancer (NSCLC). Here, a sandwich-type electrochemical immunosensor for ultrasensitive detection of CYFRA21-1 is developed. The sensor based on a combination of gold nanoparticle (AuNPs) decorated Ti3C2Tx-MXene (Au-Ti3C2Tx) as the substrate enhancer, and toluidine blue (TB) modified AuNPs doped covalent organic framework (COF) polymer as the signal tag (TB-Au-COF). The Au-Ti3C2Tx is used to capture numerous primary antibodies and accelerate the electron transfer rate of the substrate, while the TB-Au-COF can be applied to provide a large number of signal units TB and secondary antibodies. These features of composites endow the proposed immunosensor with high sensitivity and current response to CYFRA21-1. Under optimum conditions, the immunosensor offers a wide current response for CYFRA21-1 from 0.5-1.0 × 104 pg·mL-1 with a detection limit of 0.1 pg·mL-1. Furthermore, the biosensing platform can be applied for CYFRA21-1 detection to analyze real serum samples, providing an effective and useful avenue for the applicability of Au-Ti3C2Tx and TB-Au-COF composite materials in biosensing field.
Assuntos
Antígenos de Neoplasias/sangue , Técnicas Biossensoriais/métodos , Queratina-19/sangue , Estruturas Metalorgânicas/química , Titânio/química , Anticorpos Imobilizados/química , Antígenos de Neoplasias/análise , Carcinoma Pulmonar de Células não Pequenas/sangue , Técnicas Eletroquímicas/métodos , Ouro/química , Humanos , Imunoensaio/métodos , Queratina-19/análise , Limite de Detecção , Neoplasias Pulmonares/sangue , Nanopartículas Metálicas/químicaRESUMO
As a nonspecific phosphomonoesterase, alkaline phosphatase (ALP) plays a pivotal role in tissue mineralization and osteogenesis which is an important biomarker for the clinical diagnosis of bone and hepatobiliary diseases. Herein, we described a novel electrochemical method that used aminoferrocene (AFC) as an electroactive probe for the ALP activity detection. In the condition with imidazole and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), the AFC probe could be directly labeled on single-stranded DNA (ssDNA) by one-step conjugation. Specifically, thiolated ssDNA at 3'-terminals was modified to the electrode surface through Au-S bond. In the condition without ALP, AFC could be labeled on ssDNA by conjugating with phosphate groups. In the presence of ALP, phosphate groups were catalyzed to be removed from the 5'-terminal of ssDNA. The AFC probe cannot be labeled on ssDNA. Thus, the electrochemical detection of ALP activity was achieved. Under optimal conditions, the strategy presented a good linear relationship between current intensity and ALP concentration in the range of 20 to 100 mU/mL with the limit of detection (LOD) of 1.48 mU/mL. More importantly, the approach rendered high selectivity and satisfactory applicability for ALP activity detection. In addition, this method has merits of ease of operation, low cost, and environmental friendliness. Thus, this strategy presents great potential for ALP activity detection in practical applications. An easy, sensitive and reliable strategy was developed for the detection of alkaline phosphatase activity via electrochemical "Signal off".
Assuntos
Fosfatase Alcalina/análise , DNA de Cadeia Simples/análise , Eletroquímica/métodos , Enzimas/química , Compostos Ferrosos/química , Metalocenos/química , Fosfatase Alcalina/sangue , Animais , Técnicas Biossensoriais , Catálise , Bovinos , DNA de Cadeia Simples/sangue , Enzimas/sangue , Compostos Ferrosos/sangue , Glucose Oxidase/análise , Ouro/química , Humanos , Imidazóis/análise , Limite de Detecção , Metalocenos/sangue , Fosforilação , Reprodutibilidade dos Testes , Soro/química , Soroalbumina Bovina/análise , Enxofre/químicaRESUMO
Improving the sensitivity of detection is crucial to monitor biomarker, assess toxicity, and track therapeutic agent. Herein, a sensitivity-improved immunosensor is reported for the first time via functionalized graphene oxide (GO) and a "grafting-to" ring-opening polymerization (ROP) dual signal amplification strategy. Through the ROP reaction using 2-[(4-ferrocenylbutoxy)methyl] oxirane (FcEpo) as the monomer, lots of electroactive tags are linked in situ from multiple initiation sites on the GO surface modified with ethanol amine (GO-ETA), thereby achieving high sensitivity even in the case of trace amounts of tumor markers. The utmost important factor for achieving this high sensitivity is to select functionalized GO as the initiator that contains a large number of repeated hydroxyl functional groups so as to trigger additional ROP reaction. Under the optimal conditions, the high sensitivity and applicability is demonstrated by the use of GO-ETA-mediated ROP-based immunosensor to detect non-small cell lung cancer (NSCLC)-specific biomarker down to 72.58 ag/mL (equivalent to ~6 molecules in a 5 µL sample). Furthermore, the satisfactory results for the determination of biomarkers in clinical serum samples highlighted that this immunosensor holds a huge potential in practical clinical application. This work described an electrochemical immunosensor for ultrasensitive detection of CYFRA 21-1 via the functionalized graphene oxide (GO) and a "grafting-to" ring-opening polymerization (ROP) dual signal amplification strategy, which hold the merits of high sensitivity, applicability, selectivity, efficiency, easy operation and environmental friendliness.
Assuntos
Antígenos de Neoplasias/sangue , Biomarcadores Tumorais/sangue , Grafite/química , Queratina-19/sangue , Fragmentos de Peptídeos/análise , Anticorpos Imobilizados/imunologia , Antígenos de Neoplasias/imunologia , Biomarcadores Tumorais/imunologia , Técnicas Eletroquímicas/métodos , Humanos , Imunoensaio/métodos , Queratina-19/imunologia , Limite de Detecção , Reprodutibilidade dos TestesRESUMO
The cytokeratin fragment antigen 21-1 (CYFRA 21-1) protein is a critical tumor biomarker tightly related to non-small cell lung cancer (NSCLC). Herein, we prepared an effective electrochemiluminescence (ECL) immunosensor for CYFRA 21-1 detection using electrochemically mediated atom transfer radical polymerization (eATRP). The CYFRA 21-1 antigen was fixed on the electrode surface by constructing a sandwich type antibody-antigen-antibody immune system. The sensitivity of ECL was improved by using the eATRP reaction. In this method, eATRP was applied to CYFRA 21-1 detection antibody with N-acryloyloxysuccinimide as functional monomer. This is the first time that ECL and eATRP signal amplification technology had been combined. Under the optimized testing conditions, the immunosensor showed a good linear relation in the range from 1 fg mL-1 to 1 µg mL-1 at a limit of detection of 0.8 fg mL-1 (equivalent to ~ 134 molecules in a 10 µL sample). The ECL immunosensing system based on eATRP signal amplification technology provided a new way for rapid diagnosis of lung cancer by detecting CYFRA 21-1. The paper prepared an electrochemiluminescence biosensor for ultrasensitive detection of CYFRA 21-1 via eATRP signal amplification strategy, which had the advantages of high sensitivity, reproducibility, and held potential prospect for analysis of low-abundance.
Assuntos
Antígenos de Neoplasias/sangue , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Imunoensaio/métodos , Queratina-19/sangue , Acrilatos/química , Anticorpos Imobilizados/imunologia , Antígenos de Neoplasias/imunologia , Técnicas Biossensoriais/instrumentação , Técnicas Eletroquímicas/instrumentação , Eletrodos , Humanos , Imunoensaio/instrumentação , Queratina-19/imunologia , Limite de Detecção , Luminescência , Substâncias Luminescentes/química , Luminol/química , Polimerização , Reprodutibilidade dos Testes , Succinimidas/químicaRESUMO
Convenient and sensitive detection of biomolecules is of great significance to disease diagnosis. In this work, a metal-free photoinduced atom transfer radical polymerization (photoATRP) by a reductive quenching pathway as a novel strategy is applied to achieve lung cancer DNA detection. Thiolated PNA is exploited to specifically recognize target DNA, and the initiator of photoATRP is linked to the electrode surface via phosphate-Zr4+ -carboxylate. Under the excitation of blue light, the reductive quenching pathway is activated with eosin Y (EY) as photoredox catalyst and N,N,N',N'',N'-pentamethyldiethylenetriamine (PMDETA) as electron donor, and numerous polymeric chains are formed. Under optimal conditions, the linear range of this strategy is from 0.1â pm to 10â nm (R2 =0.989) with a limit of detection (LOD) of 1.4â fm (14â zmol in 10â µL). The variety of possible light sources for photoATRP and simple operation endow this biosensor with great potential for practical applications.
Assuntos
Técnicas Biossensoriais/métodos , DNA/química , Radicais Livres/química , Neoplasias Pulmonares/genética , Metais/química , Polímeros/química , Catálise , DNA/genética , Eletrodos , Humanos , Limite de Detecção , Neoplasias Pulmonares/química , PolimerizaçãoRESUMO
An ultrasensitive fluorescence biosensor for detecting cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA of non-small cell lung carcinoma (NSCLC) is designed using polysaccharide and activator regenerated by electron transfer atom transfer radical polymerization (ARGET ATRP) signal amplification strategy. Thiolated peptide nucleic acid (PNA) is fixed on magnetic nanoparticles (MNPs) by a cross-linking agent and hybridized with CYFRA 21-1 DNA. Hyaluronic acid (HA) is linked to PNA/tDNA heteroduplexes in the form of carboxy-Zr4+-phosphate. Subsequently, multiple 2-bromo-2-methylpropionic acid (BMP) molecules are linked with HA to initiate ARGET ATRP reaction. Finally, a large number of fluorescein o-acrylate (FA) monomers are polymerized on the macro-initiators, and the fluorescence signal is significantly amplified. Under optimal conditions, this biosensor shows a significant linear correlation between the fluorescence intensity and logarithm of CYFRA 21-1 DNA concentration (0.1 fM to 0.1 nM), and the limit of detection is as low as 78 aM. Furthermore, the sensor has a good ability to detect CYFRA 21-1 DNA in serum samples and to recognize mismatched bases. It suggests that the strategy has broad application in early diagnosis by virtue of its high sensitivity and selectivity. Graphical abstract A novel and highly sensitive fluorescence biosensor for quantitatively detecting CYFRA 21-1 DNA via dual signal amplification of hyaluronic acid and ARGET ATRP reaction was developed. This proposed method has a low detection limit, wide detection range, high selectivity, and strong anti-interference.
Assuntos
Antígenos de Neoplasias/sangue , Técnicas Biossensoriais/métodos , Carcinoma Pulmonar de Células não Pequenas/sangue , DNA/sangue , Queratina-19/sangue , Neoplasias Pulmonares/sangue , Espectrometria de Fluorescência/métodos , Antígenos de Neoplasias/genética , Carcinoma Pulmonar de Células não Pequenas/genética , DNA/genética , Humanos , Queratina-19/genética , Limite de Detecção , Neoplasias Pulmonares/genética , Nanopartículas de Magnetita/química , Hibridização de Ácido Nucleico , Ácidos Nucleicos Peptídicos/química , Polimerização , Polissacarídeos/químicaRESUMO
In this work, a new method of CYFRA21-1 DNA (tDNA) detection based on electrochemically mediated atom transfer radical polymerization (e-ATRP) and surface-initiated reversible addition-fragmentation chain transfer polymerization (SI-RAFT) cascade polymerization and AgNP deposition is proposed. Firstly, the peptide nucleic acid (PNA) probe is captured on a gold electrode by Au-S bonds for specific recognition of tDNA. After hybridization, PNA/DNA strands provide high-density phosphate groups for the subsequent ATRP initiator by the identified carboxylate-Zr4+-phosphate chemistry. Then, a large number of monomers are successfully grafted from the DNA through the e-ATRP reaction. After that, the chain transfer agent of SI-RAFT and methacrylic acid (MAA) are connected by recognized carboxylate-Zr4+-carboxylate chemistry. Subsequently, through SI-RAFT, the resulting polymer introduces numerous aldehyde groups, which could deposit many AgNPs on tDNA through silver mirror reaction, causing significant amplification of the electrochemical signal. Under optimal conditions, this designed method exhibits a low detection limit of 0.487 aM. Moreover, the method enables us to detect DNA at the level of PCR-like and shows high selectivity and strong anti-interference ability in the presence of serum. It suggests that this new sensing signal amplification technology exhibits excellent potential of application in the early diagnosis of non-small cell lung cancer (NSCLC). Graphical abstract Electrochemical detection principle for CYFRA21-1 DNA based on e-ATRP and SI-RAFT signal amplification technology.
Assuntos
Antígenos de Neoplasias/genética , Técnicas Biossensoriais/métodos , DNA/sangue , Queratina-19/genética , Nanopartículas Metálicas/química , Ácidos Nucleicos Peptídicos/química , Prata/química , Carcinoma Pulmonar de Células não Pequenas/sangue , Carcinoma Pulmonar de Células não Pequenas/genética , DNA/genética , Técnicas Eletroquímicas/métodos , Eletrodos , Ouro/química , Humanos , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/genética , Hibridização de Ácido Nucleico , Ácidos Nucleicos Peptídicos/genética , PolimerizaçãoRESUMO
Herein, a novel aptasensor was constructed for ultrasensitive detection of bisphenol A (BPA). In this method, an electrochemically mediated atom transfer radical polymerization (eATRP) signal amplification strategy was applied to BPA detection for the first time. The 5'-end modified sulfhydryl group and the 3'-end modified azide group hairpin DNA were immobilized on a gold electrode through an Au-S bond. The double-stranded DNA was formed by the hybridization of an aptamer and a single-stranded DNA partially paired with the hairpin DNA. In the presence of BPA, the aptamer combined with BPA and the single-stranded DNA was released to open the hairpin structure, making the azide groups at the 3' end exposed. Subsequently the initiator of eATRP was introduced into hairpin DNA through click chemistry reaction and eATRP was conducted for the polymerization of the electroactive probe ferrocene methyl methacrylate (FMMA). As a result, the ultrasensitive detection of BPA was realized, and the detection limit of this aptasensor was as low as 59 aM and a good selectivity was obtained in the presence of 100-fold structural analogs. The application of this aptasensor was evaluated by detecting BPA in pure water samples, and recoveries were in the range of 95.23-98.40%, holding promising applications in biological analysis.
Assuntos
Aptâmeros de Nucleotídeos/química , Compostos Benzidrílicos/análise , DNA de Cadeia Simples/química , Fenóis/análise , Aptâmeros de Nucleotídeos/genética , Técnicas Biossensoriais/métodos , DNA de Cadeia Simples/genética , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Compostos Ferrosos/química , Ouro/química , Sequências Repetidas Invertidas , Limite de Detecção , Metilmetacrilato/química , Hibridização de Ácido Nucleico , Polimerização , Reprodutibilidade dos Testes , Poluentes Químicos da Água/análiseRESUMO
Given the gigantic harmfulness of bisphenol A (BPA), a novel and ultrasensitive aptasensor, which employs the truncated BPA aptamer, click chemistry, and activators generated by electron transfer for atom transfer radical polymerization (AGET ATRP), was developed herein for the quantitative determination of BPA. Firstly, hairpin DNAs (hairpins) with a thiol at the 5' end and an azide group at the 3' end were conjugated with aminated magnetic beads (MBs) through heterobifunctional cross-linkers. BPA truncated aptamer (ssDNA-A) hybridizes with its complementary single-stranded DNA (ssDNA-B) to form double-stranded DNA. In the presence of BPA, ssDNA-A specifically captures BPA, and then ssDNA-B is released. Subsequently, the ssDNA-B hybridizes with hairpins to expose the azide group near the surface of the MBs. Then, propargyl-2-bromoisobutyrate (PBIB), the initiator of AGET ATRP containing alkynyl group, was conjugated with azide group of hairpins via the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Consequently, a large number of fluorescein-o-acrylate (FA) were introduced to the MBs through AGET ATRP, resulting in that the fluorescence intensity was increased dramatically. Obviously, the fluorescence intensity was especially sensitive to the change of BPA concentration, and this method can be used in quantitative determination of BPA. Under optimal conditions, a broad liner range from 100 fM to 100 nM and a low limit of detection (LOD) of 6.6 fM were obtained. Moreover, the method exhibits not only excellent specificity for BPA detection over BPA analogues but high anti-interference ability in real water sample detection, indicating that it has huge application prospect in food safety and environment monitoring.
Assuntos
Aptâmeros de Nucleotídeos/química , Compostos Benzidrílicos/análise , Fenóis/análise , Poluentes Químicos da Água/análise , Limite de Detecção , Reprodutibilidade dos Testes , Espectrometria de FluorescênciaRESUMO
A core-shell structured magnetic polyimide composite has been synthesized by the covalent coating of a mesoporous polyimide polymer onto the surface of magnetite nanoparticles. The nanocomposite was characterized by scanning electron microscopy, transmission electron microscopy, N2 adsorption-desorption isotherms, X-ray diffraction, infrared spectroscopy, and vibrating sample magnetometry. The results showed that the prepared composite had a large surface area (306.45 m²/g), a unique pore size (2.15 nm), and strong magnetic properties (45.7 emµ/g), rendering it a promising sorbent material for magnetic solid-phase extraction. The parameters that affect the extraction efficiency of rhodamine B were optimized with the assistance of response surface methodology. Under the optimal conditions, the developed method has been successfully applied to determine the rhodamine B in food samples. The linearities and limits of detection of rhodamine B in hot pepper, red wine, and chili powder samples were measured. Satisfactory recoveries in the range of 94.8-103.3% with relative standard deviations <5.5% were obtained. Investigation of the adsorption mechanism of magnetic polyimide composite indicated that multiple interactions, including hydrophobic, π-π, and hydrogen bonding interactions, were involved in the adsorption process.
Assuntos
Capsicum/química , Magnetismo/métodos , Nanocompostos/química , Rodaminas/isolamento & purificação , Extração em Fase Sólida/métodos , Vinho/análise , Adsorção , Corantes Fluorescentes , Contaminação de Alimentos/análise , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Magnetismo/instrumentação , Porosidade , Pós/química , Resinas Sintéticas/química , Rodaminas/química , Extração em Fase Sólida/instrumentaçãoRESUMO
The article describes an on-chip amplification scheme initiated by a terminal deoxynucleotidyl transferase (TdT) for highly sensitive fluorometric determination of protein. Two thrombin-binding aptamers were designed to capture thrombin as they can form a sandwich structure for improved specificity. An amino-modified aptamer (TBA29) was first immobilized on a silicon chip. After capture of thrombin, a second aptamer (TBA15) was conjugated to the second binding site of thrombin. The 3'-terminal of aptamer TBA15 is exposed on the chip surface, and then fluorescein-labeled 12-dATP associates to the 3'-terminal with the help of TdT. This results in signal amplification, and eventually leads to highly sensitive detection. Under optimal conditions, fluorescence intensity is linearly related to the logarithm of thrombin concentration in the range of 100 fM - 0.1 µM, and the detection limit is as low as 2.0 fM. The assay is sensitive and selective even over potentially interfering proteins and in the presence of human serum. Graphical abstract Schematic strategy for thrombin detection. Two thrombin-binding aptamers were designed to capture thrombin to form a sandwich structure for improved specificity. The protein detection is based on TdT initiated on-chip fluorescent amplification.
Assuntos
Técnicas Biossensoriais/métodos , DNA Nucleotidilexotransferase/metabolismo , Fluorometria/métodos , Limite de Detecção , Trombina/análise , Aptâmeros de Nucleotídeos/metabolismo , Humanos , Silício/química , Trombina/metabolismoRESUMO
BACKGROUND: Patients undergoing total knee arthroplasty (TKA) need to tolerate the effects of noise. MATERIALS AND METHODS: This study retrospectively analyzed the clinical data of 167 TKA patients at The Affiliated Hospital of Southwest Medical University from April 2019 to April 2021. A total of 154 patients who met inclusion criteria were divided into the conventional noise reduction management group (CMG) and the noise reduction earplug group (EPG), following different management schemes. The CMG received routine noise reduction management after surgery, while the EPG used noise reduction earplugs based on the CMG. The clinical indexes of the two groups were compared. RESULTS: In this study, 79 patients were included in the CMG, and 75 patients were included in the EPG. The results showed that the Pittsburgh Sleep Quality Index (PSQI) scores of both groups 2 weeks after surgery were significantly lower than those before management (ZEPG = 5.995, ZCMG = 4.109, all P < 0.001), and the EPG exhibited a significantly lower PSQI score than the CMG (Z = -2.442, P < 0.05). Two weeks after surgery, the EPG had significantly lower levels of systolic blood pressure (ZSBP = -4.303) and diastolic blood pressure (ZDBP = -3.115), as well as lower scores on the Hospital Anxiety and Depression Scale-Anxiety (HADS-A; ZHADS-A = -7.140) and Hospital Anxiety and Depression Scale-Depression (HADS-D; ZHADS-D = -4.545) compared to the CMG (all P < 0.05). In addition, no significant correlation existed between the duration of wearing earplugs and the HADS-A and HADS-D scores (r = -0.201, r = -0.002, P > 0.05). CONCLUSION: Noise reduction earplugs can improve sleep quality and regulate negative emotions of patients undergoing TKA treatment through a complex mechanism involving noise, which is beneficial to the prognosis of the disease.