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The overdose of acetaminophen (AP) can cause serious acute liver injury even the irreversible liver necrosis. The quantitative detection of AP is of great significance not only for clinical applications but also for the quantity control of its pharmaceutical formulations. In this paper, a sensitive molecularly imprinted voltammetric sensor towards AP was constructed based on synergistic enhancement of nitrogen-vacancy graphitized carbon nitride (NV-g-C3 N4 ) and carboxylated MWCNTs loaded with silver nanoparticles (Ag-MWCNTs). The powder X-Ray diffraction spectrum, field emission scanning and transmission electron microscopes, cyclic voltammetry (CV), and electrochemical impedance spectrum were used to characterize the composites. The results show that NV-g-C3 N4 and Ag-MWCNTs closely embedded each other, forming loose porous hybrid structure by hydrogen bond. The prepared sensor molecular imprinting polymer (MIP)/C3 N4 /Ag-CNTs/GCE shows a strong synergistic enhancement of electroanalytical response by CV and differential pulse voltammetry (DPV) tests when compared with NV-g-C3 N4 /GCE, Ag-CNTs/GCE, and MIP/GCE. Through the optimization of the ratio of monomer and template, electropolymerization cycle, elution cycle, incubation time, and pH, linear ranges of 0.007-5 and 5-100 µM were found with the limit of detection of 2.33 nM by DPV. Moreover, its selectivity towards AP was satisfied when compared with detection towards ascorbic acid, dopamine, and glucose. The recovery range of 96.3%-100.5% was obtained in the spiked human serum and urine samples with the SD below 3.0%. In addition, the prepared sensor shows great detecting robustness with good anti-interference, reproducibility, and stability.
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Nanopartículas Metálicas , Impressão Molecular , Humanos , Técnicas Eletroquímicas/métodos , Acetaminofen , Nitrogênio , Eletrodos , Limite de Detecção , Reprodutibilidade dos Testes , Prata , Impressão Molecular/métodos , Polímeros/química , Carbono/químicaRESUMO
BACKGROUND: Evidence regarding the relationship between serum vitamin C levels and human papillomavirus (HPV) infection is limited. Therefore, this study aimed to investigate whether serum vitamin C levels are independently associated with HPV infection. METHODS: Data for this cross-sectional study were obtained from the National Health and Nutrition Examination Survey 2003-2006. A total of 2174 women, 18-59 years of age, were enrolled in this study. The associations between serum vitamin C levels (continuous and categorical forms) and cervicovaginal HPV infection were estimated using weighted logistic regression. RESULTS: The adjusted binary logistic regression showed that serum vitamin C was not associated with the risk of HPV infection after adjusting for age, race, poverty income ratio, alcohol consumption, smoking, body mass index, education, and health condition (odds ratio [OR] 0.998, 95% confidence interval [CI] 0.994-1.001). Serum vitamin C levels were converted from a continuous variable to a categorical variable for the analysis. Compared with the vitamin C deficiency and hypovitaminosis groups, there was a negative correlation between vitamin C and HPV infection when vitamin C was adequate (OR 0.7, 95% CI: 0.52-0.94); however, when the serum vitamin C level was inadequate and saturated, this negative correlation was weaker or nonexistent (OR 0.76, 95% CI 0.56-1.03 and OR 0.76, 95% CI 0.55-1.04, respectively). A nonlinear relationship was detected between vitamin C level and HPV infection. Furthermore, we performed subgroup analysis of different models and found that serum vitamin C concentration was negatively associated with HPV infection in women ≥ 25 years of age; however, in women < 25 years of age, serum vitamin C levels were not associated with HPV infection. CONCLUSION: The results from this United States nationally representative sample supported the hypothesis that there was a U-shaped relationship between serum vitamin C levels and HPV infection. Future studies are warranted to assess the association between vitamin C and HPV persistence and clarify the underlying mechanisms of these associations.
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Infecções por Papillomavirus , Estudos Transversais , Feminino , Humanos , Inquéritos Nutricionais , Razão de Chances , Fatores de Risco , Estados Unidos/epidemiologia , VitaminasRESUMO
A novel and practical palladium-catalyzed aerobic oxyarylthiolation of alkynone O-methyloximes for the assembly of 4-sulfenylisoxazole derivatives using S8 and arylhydrazines as the S-aryl sources is accomplished. In the presence of 0.1 mol% of IPr-Pd-allyl-Cl as the catalyst and O2 (1 atm) as the sole oxidant, both alkynone O-methyloximes and arylhydrazines are suitable substrates, delivering diverse 4-sulfenyl isoxazoles in moderate to good yields with good functional group tolerance. Notably, the phenyl diazonium salt and sodium phenyl sulfinate are also suitable arylation reagents, providing an alternative synthetic strategy to access structurally diverse 4-sulfenyl isoxazoles.
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Immobilized TiO2 nanotube arrays (NTAs) co-modified with Pt and CdS nanoparticles were fabricated by using the combination of photoreduction and chemical bath deposition methods. XRD, SEM, TEM, XPS, UV-Vis and EDX methods were employed to characterize the microstructure and composition of samples, and the results showed that CdS and Pt NPs were uniformly deposited on the surface of TiO2 nanotubes. The CdS/Pt/TiO2 NTAs exhibited a much higher photocatalytic activity compared to pure TiO2 NTAs and binary CdS (or Pt)/TiO2 NTAs under visible light irradiation. A kinetic study showed that the rate constants of Pt/TiO2, CdS/TiO2 and CdS/Pt/TiO2 NTAs are 0.00736, 0.01717 and 0.02077 min(-1), respectively, revealing a remarkable kinetic enhancement in the ternary heteronanostructures due to the synergistic effect of the three components. Besides, the CdS/Pt/TiO2 NTAs exhibit high stability after being used 22 times. Thus we proposed that such ternary heteronanostructures show great promise as immobilized catalysts for high efficient visible-light-driven photocatalysis.
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A simple and rapid electrochemical sensing method with high sensitivity and specificity of aptamers was developed for the detection of methylamphetamine (MAMP). A short anti-MAMP thiolated aptamer (Apt) with a methylene blue (MB) probe at 3'-end was immobilized on the surface of a gold electrode (MB-Apt-S/GE). The electrochemical signal appeared when MAMP presenting in the sample solution competed with cDNA for binding with MB-Apt-S. Under optimized conditions, the liner range of this signal-on electrochemical aptasensor for the detection of MAMP achieved from 1.0 to 10.0 nmol/L and 10.0-400 nmol/L. LOD 0.88 nmol/L were obtained. Satisfactory spiked recoveries of saliva and urine were also obtained. In this method, only 5 min were needed to incubate before the square wave voltammetry (SWV) analysis, which was much more rapid than other electrochemical sensors, leading to a bright and broad prospect for the detection of MAMP in biological sample. This method can be used for on-site rapid detection on special occasions, such as drug driving scenes, entertainment venues suspected of drug use, etc.
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Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Técnicas Eletroquímicas , Metanfetamina , Técnicas Eletroquímicas/métodos , Técnicas Biossensoriais/métodos , Aptâmeros de Nucleotídeos/química , Humanos , Metanfetamina/urina , Metanfetamina/análise , DNA Complementar/genética , Saliva/química , Saliva/metabolismo , Eletrodos , Limite de Detecção , Ouro/química , Azul de Metileno/químicaRESUMO
A facile and green method was developed to synthesize the graphene-carbon nanotubes (Gr-CNTs) nanocomposite with a sandwich lamination structure. Pt nanoparticles were loaded on the as-synthesized Gr-CNTs nanocomposite to prepare an electrochemical sensor for determining bisphenol A (BPA) in thermal printing paper. The electrochemical behavior of BPA on the Pt/Gr-CNTs nanocomposite was investigated by cyclic voltammetry (CV) and chronocoulometry (CC). The direct determination of BPA was accomplished by using differential pulse voltammetry (DPV) under optimized conditions. The oxidation peak current was proportional to the BPA concentration in the range from 6.0 × 10(-8) to 1.0 × 10(-5) M and 1.0 × 10(-5) to 8.0 × 10(-5) M with a correlation coefficient of 0.987 and 0.998, respectively. The detection limit was 4.2 × 10(-8) M (S/N = 3). The fabricated electrode showed good reproducibility, stability and selectivity. The proposed method was successfully applied to determine BPA in thermal printing papers samples and the results were satisfactory.
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Compostos Benzidrílicos/análise , Técnicas Biossensoriais , Técnicas Eletroquímicas , Disruptores Endócrinos/análise , Fenóis/análise , Compostos Benzidrílicos/química , Grafite/química , Nanopartículas Metálicas/química , Nanocompostos/química , Nanotubos de Carbono/química , Papel , Fenóis/química , Platina/químicaRESUMO
Heavy metals such as lead, mercury, and cadmium have been identified to have negative impacts on human health. Although the individual effects of these metals have been extensively researched, the present study aims to explore their combined effects and their association with serum sex hormones among adults. Data for this study were obtained from the general adult population of the 2013-2016 National Health and Nutrition Survey (NHANES) and included five metal (mercury, cadmium, manganese, lead, and selenium) exposures and three sex hormones (total testosterone [TT], estradiol [E2], and sex hormone-binding globulin [SHBG]) levels. The free androgen index (FAI) and TT/E2 ratio were also calculated. The relationships between blood metals and serum sex hormones were analysed using linear regression and restricted cubic spline regression. The effect of blood metal mixtures on sex hormone levels was examined using the quantile g-computation (qgcomp) model. There were 3,499 participants in this study, including 1,940 males and 1,559 females. In males, positive relationships between blood cadmium and serum SHBG (ß=0.049 [0.006, 0.093]), lead and SHBG (ß=0.040 [0.002, 0.079]), manganese and FAI (ß=0.080 [0.016, 0.144]), and selenium and FAI (ß=0.278 [0.054, 0.502]) were observed. In contrast, manganese and SHBG (ß=-0.137 [-0.237, -0.037]), selenium and SHBG (ß=-0.281 [-0.533, -0.028]), and manganese and TT/E2 ratio (ß=-0.094 [-0.158, -0.029]) were negative associations. In females, blood cadmium and serum TT (ß=0.082 [0.023, 0.141]), manganese and E2 (ß=0.282 [0.072, 0.493]), cadmium and SHBG (ß=0.146 [0.089, 0.203]), lead and SHBG (ß=0.163 [0.095, 0.231]), and lead and TT/E2 ratio (ß=0.174 [0.056, 0.292]) were positive relationships, while lead and E2 (ß=-0.168 [-0.315, -0.021]) and FAI (ß=-0.157 [-0.228, -0.086]) were negative associations. This correlation was stronger among elderly women (>50 years old). The qgcomp analysis revealed that the positive effect of mixed metals on SHBG was mainly driven by cadmium, while the negative effect of mixed metals on FAI was mainly driven by lead. Our findings indicate that exposure to heavy metals may disrupt hormonal homeostasis in adults, particularly in older women.
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Mercúrio , Metais Pesados , Selênio , Masculino , Humanos , Adulto , Feminino , Idoso , Pessoa de Meia-Idade , Estudos Transversais , Cádmio , Manganês , Inquéritos Nutricionais , Hormônios Esteroides Gonadais , Testosterona , EstradiolRESUMO
OBJECTIVE: We aimed to construct and validate a prognostic nomogram to predict cancer-specific survival (CSS) after surgery in patients with advanced endometrial carcinoma (EC). DESIGN: Retrospective cohort study. SETTING AND PARTICIPANTS: The Surveillance, Epidemiology, and End Results (SEER) Database contains cancer incidence and survival data from population-based cancer registries in the USA. A total of 5445 patients from the SEER Database diagnosed with advanced EC between 2004 and 2015 were included and randomised 7:3 into a training cohort (n=3812) and a validation cohort (n=1633). OUTCOME MEASURE: CSS. RESULTS: The nomograms for CSS included 10 variables (positive regional nodes, age, tumour size, International Federation of Gynecology and Obstetrics (FIGO) stage, grade, ethnicity, income, radiation, chemotherapy and historical stage) based on the forward stepwise regression results. They revealed discrimination and calibration using the concordance index (C-index) and area under the time-dependent receiver operating characteristic curve, with a C-index value of 0.7324 (95% CI=0.7181 to 0.7468) and 0.7511 (95% CI=0.7301 to 0.7722) for the training and validation cohorts, respectively. Using calibration plots, a high degree of conformance was shown between the predicted and observed results. Additionally, a comparison of the nomogram and FIGO staging based on changes in the C-index, net reclassification index and integrated discrimination improvement demonstrated that the nomogram had better accuracy and efficacy. CONCLUSIONS: We successfully constructed an accurate and effective nomogram to predict CSS in patients with advanced EC, which may help clinicians determine optimal individualised treatment strategies for patients with advanced EC. The predictive performance of the nomogram was evaluated thoroughly, but only internally. Therefore, further validation using different data sources is warranted in future related studies.
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Neoplasias do Endométrio , Nomogramas , Feminino , Gravidez , Humanos , Prognóstico , Estudos Retrospectivos , Neoplasias do Endométrio/cirurgia , CalibragemRESUMO
Trace amounts of Co3O4 modified TiO2 nanorod arrays were successfully fabricated through the photochemical deposition method without adding any nocuous reagents. The Co3O4/TiO2 nanorod arrays fabricated in acid solution had the highest photo-electrochemical activity. We elaborated on the mechanism of Co3O4-TiO2 fabricated in different pH value solutions. The Co3O4-TiO2 had a more remarkable photo-electrochemical performance than the pure TiO2 nanorod arrays owing to the heterojunction between Co3O4 and TiO2. The degradation of methylene blue and hydroquinone was selected as the model reactions to evaluate the photo-electrochemical performance of Co3O4-TiO2 nanorod arrays. The Co3O4/TiO2 nanorod arrays had great potential in waste water treatment.
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MicroRNAs (miRNAs) play an important role in multiple biological processes and can be used as biomarkers for clinical disease diagnosis, so their detection is of great importance. Here, manganese dioxide (MnO2) nanosheet acts as carrier to deliver DNAzyme probes into cells through endocytosis, where intracellular glutathione (GSH) reduces the MnO2 nanosheet to manganese ions (Mn2+) and releases the probes. The generated Mn2+ can be further used as an effective cofactor to activate the DNAzyme probe, and cleave the DNA strand into two fragments. Then, the miRNA-155 in the cells can hybridize with the cleaved fragment to cause the fluorescence signal change of the probe. The proposed proportional fluorescent method has been applied to the imaging of miRNA-155 in HeLa cells and HepG2 cells with the estimated detection limit (LOD) as 1.6 × 10-12 M. The new method can provide great help for cancer diagnosis and biological research related to miRNA.
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DNA Catalítico , MicroRNAs , DNA Catalítico/genética , Corantes Fluorescentes , Células HeLa , Humanos , Compostos de Manganês , MicroRNAs/genética , ÓxidosRESUMO
Nanocrystalline FeAl powder is synthesized by subsequent heat treating the Fe50Al50 (at.%) alloy prepared by mechanical alloying. During annealing the milled FeAl powder, the grain growth of B2-FeAl occurs with the order transformation from Fe(Al) to B2-FeAl. The activation energy for the nanocrystalline FeAl growth is calculated to be 534.9 kJ/mol, according to the kinetics theory of nanocrystalline growth. The grain growth of FeAl is significantly inhibited especially at elevated temperature.
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In clinical practice, the excess concentration of bilirubin can trigger diseases such as neonatal jaundice, hepatic failure, septicemia, and so on. The concentration of bilirubin is one of important clinical indexes to evaluate patients with hepatic function disease in clinical practice. Therefore, it is very necessary to develop a rapid detection technique detecting the bilirubin in body fluids. Here, a new electrochemical sensor based on Au nanoparticles/tetrathiafulvalene-carboxylate functionalized reduced grapheneoxide 0D-2D heterojunction(AuNPs/TTF-COOH/RGO) was fabricated for the discrimination of bilirubin in real human blood. The TTF-COOH could effectively repair electron conductivity of RGO nanosheets, decrease interface resistance, and also enhance the dispersity of TTF-COOH/RGO nanosheets in water. What's more, the S atoms of TTF-COOH can bonding the gold nano-particles (AuNPs) to fabricate a 0D-2D heterojunction with excellent biocompatibility and enhanced specific surface area. After bilirubin oxidases were self-assembled on the surface of AuNPs, a specific recognition interface was formed as a sensor for the detection of bilirubin. The heterojunction showed enhanced interface electron transfer rate, excellent biocompatibility, and also prominent electrocatalytic activity for the high efficiency catalysis of bilirubin. The sensor shows a linear response for bilirubin from 2.66 to 83â¯â¯µmolâ¯L-1 and a low detection limit of 0.74⯵molâ¯L-1 at 3σ. This work provides one novel approach to detection of bilirubin by functional RGO nanosheets, and broadens the application area of RGO nanosheets in selective catalysis and detection of biomolecule in biological specimens, such as blood, urine.
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Bilirrubina/sangue , Ácidos Carboxílicos/química , Técnicas Eletroquímicas , Ouro/química , Grafite/química , Compostos Heterocíclicos/química , Nanopartículas Metálicas/química , Eletrodos , Humanos , Prata/químicaRESUMO
The development of efficient solar driven catalytic system for the degradation of antibiotics has become increasingly important in environmental protection and remediation. Non-noble-metal NiS and MoS2 nanosheet co-modified graphitic C3N4 ternary heterostructure has been synthesized via a facile combination of hydrothermal and ultrasound method, and the ternary heterostructure has been utilized for photocatalytic degradation of antibiotic agents. The antibiotics of ciprofloxacin (CIP) and tetracycline hydrochloride (TC) were photodegraded by the hybrid under the visible light. The optimal photodegradation rate of the ternary heterostructure reaches about 96% after 2h irradiation, which is 2.1 times higher than that of pure g-C3N4 for TC degradation. The photocatalytic degradation rates of the ternary heterostructure for both CIP and TC obey the pseudo-first-order kinetic model. The enhanced visible light adsorption and charge separation efficiency contribute to the photocatalytic performance of the ternary heterostructure. This work provides new insights and pathways by which efficient degradation of antibiotics can be achieved and will stimulate further studies in this important field.
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Antibacterianos/química , Dissulfetos , Grafite , Luz , Molibdênio , Nanocompostos , Níquel , Nitrilas , Compostos Azo/química , Ciprofloxacina/química , Corantes/química , Dissulfetos/química , Dissulfetos/efeitos da radiação , Grafite/química , Grafite/efeitos da radiação , Molibdênio/química , Molibdênio/efeitos da radiação , Nanocompostos/química , Nanocompostos/efeitos da radiação , Níquel/química , Níquel/efeitos da radiação , Nitrilas/química , Nitrilas/efeitos da radiação , Fotólise , Tetraciclina/químicaRESUMO
Aflatoxin B1 (AFB1) is the most toxic among the mycotoxins and causes detrimental health effects on human and animals. Selenium (Se) plays an important role in chemopreventive, antioxidant, anticarcinogen, and detoxification and involved in cell cycle regulation. The aim of this study was to explore the molecular mechanisms of selenium involved in inhibition of G2/M cell cycle arrest of broiler's jejunum. A total of 240 one-day-old healthy Cobb broilers were randomly divided into four groups and fed with basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se (AFB1 + Se group) for 21 days, respectively. The histological observation and morphological analysis revealed that 0.4 mg/kg Se prevented the AFB1-associated lesions of jejunum including the shedding of the apical region of villi, the decreased villus height, and villus height/crypt ratio. The cell cycle analysis by flow cytometry showed that 0.4 mg/kg Se ameliorated the AFB1-induced G2/M phase arrest in jejunal cells. Moreover, the expressions of ATM, Chk2, p53, Mdm2, p21, PCNA, Cdc25, cyclin B, and Cdc2 analyzed by immunohistochemistry and qRT-PCR demonstrated that 0.4 mg/kg Se restored these parameters to be close to those in the control group. In conclusion, Se promoted cell cycle recovery from the AFB1-induced G2/M phase arrest by the molecular regulation of ATM pathway in the jejunum of broilers. The outcomes from the present study may lead to a better understanding of the nature of selenium's essentiality and its protective roles against AFB1.
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Aflatoxina B1/antagonistas & inibidores , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Jejuno/efeitos dos fármacos , Selênio/farmacologia , Aflatoxina B1/toxicidade , Animais , Galinhas , Suplementos Nutricionais , Selênio/administração & dosagemRESUMO
Aflatoxin B1 (AFB1), one of most potent and common mycotoxins in human food and animal feed, has hepatotoxic and carcinogenic effects on humans and poultry. Recent studies indicated that selenium (Se) has a protective effect on apoptosis induced by toxin poisoning. The present study was designed to reveal the ameliorative effects of selenium on the expression of apoptosis related molecules in the jejunum of broilers exposed to an AFB1 diet for 3 weeks. A total of 216 one-day-old healthy Cobb broilers were randomly divided into the control group (0 mg kg-1 AFB1), AFB1 group (0.6 mg kg-1 AFB1), AFB1 + Se group (0.6 mg kg-1 AFB1 + 0.4 mg kg-1 supplement Se) and Se group (0.4 mg kg-1 supplement Se), respectively. TUNEL and flow cytometry assays both indicated that 0.4 mg kg-1 selenium could ameliorate excess apoptosis caused by AFB1 in jejunal cells. Moreover, the expressions of FAS, FASL, TNF-α, TNF-R1, CASPASE-3, CASPASE-8, CASPASE-10, GRP78 and GRP94 analyzed by qRT-PCR demonstrated that 0.4 mg kg-1 selenium restored these parameters to be close to those in the control group. In summary, supplementation of selenium at a concentration of 0.4 mg kg-1 selenium could protect the chicken's jejunum from excess apoptosis caused by 0.6 mg kg-1 AFB1 through down-regulating the expression of death receptor pathway and endoplasmic reticulum pathway related molecules. According to this conclusion, this study may contribute to a better understanding of selenium's protective role against AFB1 poisoning.
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Aflatoxin B1 (AFB1), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of mucosal humoral response in the cecal tonsil generated by AFB1. A total of 156 one-day-old broilers were divided into four groups and fed on basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se supplement (AFB1+Se group) respectively for 21 days. Our results showed that 0.4 mg/kg Se supplement in broiler's diets could improve the AFB1-induced histological lesions in the cecal tonsils including the depletion of lymphocytes in the lymphatic nodules as well as the shedding of microvilli in the absorptive cells. Moreover, Se could restore the decreased number of IgA+ cells and expression levels of pIgR, IgA, IgG, and IgM mRNA induced by AFB1 to be close to those in the control group. These results demonstrated that 0.4 mg/kg supplemented dietary Se in the form of sodium selenite could protect the cecal tonsils from the histological lesions and suppression of the mucosal humoral response provoked by 0.6 mg/kg AFB1. Our study may provide new experimental evidences for better understanding of AFB1-induced damage of mucosal immunity and protective effect of Se against this toxin.
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Aflatoxin B1 (AFB1) is a natural product of the Aspergillus genus of molds, which grow on several foodstuffs stored in hot moist conditions, and is among the most potent hepatocarcinogens and immunosuppression presently known. The latter was related to the up-regulated apoptosis of immune organs. However, the effect of expression of death receptor and endoplasmic reticulum molecules in AFB1-induced apoptosis of chicken splenocytes was largely unknown. The objective of this study was to investigate this unknown field. One hundred and forty four one-day-old chickens were randomly divided into control group (0 mg/kg AFB1) and AFB1 group (0.6 mg/kg AFB1), respectively and fed with AFB1 for 21 days. Histological observation demonstrated that AFB1 caused slight congestion and lymphocytic depletion in the spleen. TUNEL and flow cytometry assays showed the excessive apoptosis of splenocytes provoked by AFB1. Moreover, quantitative real-time PCR analysis revealed that AFB1 induced the elevated mRNA expression of Fas, FasL, TNF-α, TNF-R1, Caspase-3, Caspase-8, Caspase-10, Grp78 and Grp94 in the spleen. These findings suggested that AFB1 could lead the excessive apoptosis and alter the expression of death receptor and endoplasmic reticulum molecules in chicken spleen.
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Aflatoxin B1 (AFB1) is a common contaminant of poultry feeds in tropical and subtropical climates. Early researches have well established the hepatotoxic, carcinogenic, and immunotoxic effects of AFB1 on humans and animals. Recently, it has been shown that AFB1 could cause the up- or down-alteration of mitochondrial pathway molecule expression. However, the information on the expression of death receptor and endoplasmic reticulum molecules in the jejunal apoptosis induced by AFB1 were unavailable. So the present study was conducted to explore the expression of apoptotic molecules related to death receptor and endoplasmic reticulum in the jejunal cells of chickens exposed to AFB1 diet for 3 weeks. Total of 144 one-day-old chickens was randomly divided into two groups, namely control group (containing 0 mg/kg AFB1) and AFB1 group (containing 0.6 mg/kg AFB1). Histopathological observation and microscopic quantitative analysis revealed morphological changes in the jejunum such as the shedding of the mucosal epithelial cells in the apical region of villi along with the decrease of villus height, villus area and villus/crypt ratio in the AFB1 group. Both TUNEL and flow cytometry assays showed that AFB1 intake induced excessive apoptosis of jejunal cells. Quantitative real-time PCR test displayed the general upregulation of death receptors (FAS, FASL, TNF-α and TNF-R1), endoplasmic reticulum signals (GRP78 and GRP94) as well as initiator and executioner caspases (CASPASE-10, CASPASE-8 and CASPASE-3) in the jejunum of AFB1-intoxicated chickens. It's the first study demonstrating that AFB1 induced apoptosis of chickens' jejunum accompanied by the alteration of death receptor and endoplasmic reticulum molecule expression.
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Graphitic C3N4/ultrathin MoS2 (MoS2/g-C3N4) hybrids were synthesized via a facile bathing and ultrasound method. In this process, a well-bonded interface structure was formed between ultrathin MoS2 nanosheets and g-C3N4 through adjusting the amount of MoS2 in the MoS2/g-C3N4 hybrids. The MoS2/g-C3N4 hybrids were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy (Raman), transmission electron microscopy (TEM) and UV-vis spectroscopy (UV-vis). The MoS2/g-C3N4 photocatalyst showed excellent photocatalytic activity in the photodegradation of organic pollutants. In this work, methyl orange (MO) was used as the simulative pollutant; the highest photodegradation rate (92.4%) was obtained when the amount of MoS2 was 5 wt% in the MoS2/g-C3N4 hybrids (0.05-MC), with a kinetic constant of 0.0189 min-1 after being irradiated under visible light for 2 h. Besides, 0.05-MC also showed excellent recyclability and chemical stability, and a photodegradation rate of 79.93% was reached after being reused 10 times. For practical pollutants, the photocatalytic degradation rates of ciprofloxacin (CIP) and tetracycline hydrochloride (TC) have been increased dramatically under the visible light irradiation. The excellent photocatalytic properties of MoS2/g-C3N4 hybrids can be ascribed to the enhanced separation rate and accelerated mobility of photogenerated charges through ultrathin MoS2 nanosheets' modification.
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Novel mesoporous TiO2 nanotube arrays (TiO2 NTAs) were synthesized by an anodization method combined with chemical etching in HF solution, and the electrochemical performance was studied. Glucose oxidase (GOx) was immobilized on the mesoporous TiO2 NTAs to achieve an efficient biosensor for amperometric detection of glucose. The morphology, structure, component and electrochemical performance of mesoporous TiO2 NTAs were characterized by scanning electron microscopy, high resolution transmission electron microscopy, X-ray diffractometry, X-ray photoelectron spectrometry and an electrochemical workstation, respectively. The influence of the mesoporous structure on the electrochemical performance is discussed in detail by comparing the cyclic voltammograms and electrochemical impedance spectrum of TiO2 and mesoporous TiO2 NTAs in different conditions. High electrochemical active surface area and electron transfer rate play key roles in enhancing the electrochemical performance of mesoporous TiO2 NTAs. When used as the basis of a biosensor, the amperometric response of glucose on a GOx/TiO2-0.5 NTAs electrode is linearly proportion to the glucose concentration in the range from 0.1 to 6 mM with a sensitivity of 0.954 µA mM(-1) cm(-2), which is 14.3 times that of un-etched GOx/TiO2 NTAs.