Evaluation of semi-quantitative colorimetric assays based on loop-mediated isothermal amplification indicators by using image analysis.

Colorimetric assays are some of the most convenient detection methods, creating discoloration in solutions that is visible to the naked eye. However, colorimetric reactions have some limitations regarding the variability in the color perception of individuals caused by factors such as color blindness, experience, and gender. Semi-quantitative chromatic analysis has been used as an alternative method to differentiate between two colors and accurately interpret the results from a numerical value, with high confidence. Therefore, we developed and determined the optimal model between Red-Green-Blue (RGB) and Commission Internationale de l'Eclairage (CIE) Lab color spaces to establish a semi-quantitative colorimetric assay via image analysis by the ImageJ program for loop-mediated isothermal amplification (LAMP), using the dyes malachite green and phenol red. The semi-quantitative colorimetric assays using the color distance values of the CIELab color space (ΔEab) were more suitable than those using the RGB color space (ΔERGB) for chromatic differentiation between positive and negative reactions in both indicator dyes, demonstrating the feasibility of this assay to be applied in the detection of a wide range of pathogens and infectious diseases.

Masking ability of gingiva-colored resin-based composites over different tooth-colored substrates.

The purpose of the study was to investigate the influence of different tooth-colored substrates and restoration thicknesses on the final color of gingiva-colored resin-based composites (GCRBCs). Five different shades of GCRBCs [light pink (LP), dark pink (DP), orange (Or), brown (Br), and purple (P)] were used to prepare disc-shaped specimens with 2 different thicknesses: 1.0 mm, and 2.0 mm. GCRBC discs (n = 5) were placed over 3 different tooth-colored substrates (ND1, ND5, and ND9) and color parameters were assessed using a spectroradiometer. Color differences (∆E*ab and ∆E00) were calculated using CIELab and CIEDE2000 formulas and compared to 50:50% perceptibility (PT: ∆E*ab = 1.7, ΔE00 = 1.1) and acceptability (AT: ∆E*ab = 3.7, ΔE00 = 2.8) visual thresholds. Color variation data were statistically analyzed using two-way ANOVAs followed by Bonferroni's post hoc tests (a = 05). The ∆E*ab and ΔE00 values of GCRBCs placed over ND9 substrates were significantly higher in the LP-1.0 mm and Or-1.0 mm groups that presented values above AT (p< .001).Regardless of the substrate color and GCRBC thickness applied, ∆E*ab and ΔE00 values below AT were recorded in the gingival color groups of P. Substrate color significantly affected the color differences in the gingival color groups of LP, DP, and Or with a restoration thickness of 1.0 mm (p < .05). Gingival color, restoration thickness, and substrate color influenced the color differences of GCRBCs. When the gingival color was a lighter gingival color, rather than dark purple, the masking ability was decreased, especially with a restoration thickness of 1.0 mm.

Three-dimensional representation of the Vita Toothguide 3D-Master: An in vivo clinical study.

STATEMENT OF PROBLEM: The Vita Toothguide 3D-Master (Vita Zahnfabrik) is considered the dental shade guide in which the three dimensions of color - lightness, hue, and chroma - are most well-ordered in the CIELAB color space. No research has yet explored how well the Vita Toothguide 3D-Master is ordered in the 3D color space by recording color coordinates in vivo. PURPOSE: To evaluate the spatial color distribution of the Vita Toothguide 3D-Master's lightness, chroma, and hue groups and its 26 physical shade tabs. MATERIALS AND METHODS: The dental color (L*, C*, h°, a*, and b* color coordinates) of a healthy maxillary central incisor was recorded for 1361 participants (aged between 18 and 89 years) using a Vita Easyshade Compact spectrophotometer (Vita Zahnfabrik). The R 2.7.2. statistics program was used to create the visual representations. RESULTS: The five lightness levels are those that are best distributed in the color space, in relation to the L* coordinates, followed by the chroma group and, finally, the hue group. The 5M1, 5M2, and 5M3 physical shade tabs are situated at a greater distance apart from the other tabs in the color space. CONCLUSIONS: The Vita Toothguide 3D-Master's 26 physical shade tabs are satisfactorily distributed in three-dimensional space, although strict mathematical criteria are not followed. The natural dental shades that fall lower on the lightness scale are the most poorly represented by the physical shade tabs. CLINICAL IMPLICATIONS: Darker teeth are poorly represented by the Vita Toothguide 3D-Master's physical shade tabs. The spatial distribution of dental shade guides needs to be improved to ensure they provide homogeneous coverage of the entire chromatic spectrum corresponding to natural teeth. This would help reduce the errors inherent to the subjective visual color selection process.

A method for screening antifouling paints using the CIELAB coordinates of Ectocarpus sp. under a flow-through condition.

A laboratory test with a flow-through system was designed and its applicability to testing antifouling paints of varying efficacies was investigated. Six different formulations of antifouling paints were prepared to have increased contents (0 to 40 wt%) of Cu2O. The test plates were initially aged for 45 days by rotating them at a speed of 10 knots inside a cylinder drum. A bioassay was then conducted using the alga, Ectocarpus sp., as the test species. The new bioassay for screening antifouling paints under a flow-through system was successfully established using algae affixed on substrata. The correlation between the mean values of CIELAB parameters (L*, a* and b*), the total colour difference (ΔE*), and the cell survival rate of the algae was investigated. The paint performance estimated from the bioassay was confirmed using correlation patterns between colour parameters and the algal cell survival rate.

Investigating the Antioxidant and Color Properties of Bee Pollens of Various Plant Sources.

In our study, Central and Eastern European bee pollens of different botanical origins were compared, based on their antioxidant and color properties. Total phenolic content (TPC), total flavonoid content (TFC), and in vitro antioxidant capacity (by FRAP, CUPRAC, ABTS⋅+ and DPPH⋅ assays) were determined spectrophotometrically. Besides, Relative Antioxidant Capacity Indexes (RACI) were calculated. CIELAB color parameters (L*, a*, b*, chroma) were determined by using a tristimulus-based instrument. Potential correlations between the investigated parameters were also identified. Based on the results of the preliminary study, ethanol:distilled water (60 : 40) was chosen as an extraction solvent. The total phenolic content of our samples ranged between 9.41 and 27.49 mg GAE/g dw. Pollens showed TFC:TPC ratios between 9 and 44 %. RACI values indicate that rapeseed (Brassica napus), traveller's joy (Clematis vitalba) and phacelia (Phacelia tanacetifolia) pollens have relatively high, while pollens of certain plants of the Asteraceae family possess low antioxidant potential. Antioxidant properties correlated significantly in most cases. RACI values showed strong positive correlation with each of the other antioxidant capacity parameters, suggesting that this approach is well applicable for comparing the antioxidant potential of bee pollens. No clear correlation was found between the antioxidant and color parameters.

Using artificial intelligence to predict the final color of leucite-reinforced ceramic restorations.

OBJECTIVES: The aim of this study was to evaluate the accuracy of machine learning regression models in predicting the final color of leucite-reinforced glass CAD/CAM ceramic veneer restorations based on substrate shade, ceramic shade, thickness and translucency. METHODS: Leucite-reinforced glass ceramics in four different shades were sectioned in thicknesses of 0.3, 0.5, 0.7, and 1.2 mm. The CIELab coordinates of each specimen were obtained over four different backgrounds (black, white, A1, and A3) interposed with an experimental translucent resin cement using a calibrated spectrophotometer. The color change (CIEDE2000) values, as well as all the CIELab values for each one of the experimental groups, were submitted to 28 different regression models. Each regression model was adjusted according to the weights of each dependent variable to achieve the best-fitting model. RESULTS: Different substrates, ceramic shades, and thicknesses influenced the L, a, and b of the final restoration. Of all variables, the substrate influenced the final ceramic shade most, followed by the ceramic thickness and the L, a, and b of the ceramic. The decision tree regression model had the lowest mean absolute error and highest accuracy to predict the shade of the ceramic restoration according to the substrate shade, ceramic shade and thickness. CLINICAL SIGNIFICANCE: The machine learning regression model developed in the study can help clinicians predict the final color of the ceramic veneers made with leucite-reinforced glass CAD/CAM ceramic HT and LT when cemented with translucent cements, based on the color of the substrate and ceramic thicknesses.

Study of attached gingiva space color according to gender and age in Caucasian population.

OBJECTIVE: To describe the color of the healthy attached gingiva adjacent to the maxillary incisors and to evaluate the effect of age and gender on CIELAB color coordinates. MATERIALS AND METHODS: The study included 216 Caucasian individuals (129 females and 87 males) divided into three age groups. A SpectroShade Micro spectrophotometer was used to register color coordinates at 2.5 mm apical of the zenith of the upper central incisors. Descriptive and inferential statistical analysis was performed. RESULTS: The minimum and maximum coordinates in which the CIELAB natural gingival space is delimited are: L* minimum 40.4 - L* maximum 61.2; a* minimum 17.0 - a* maximum 30.2; b* minimum 9.8 - and b* maximum 21.9. There are statistically significant differences between males and females for coordinates L*, a* and b* in the attached gingival area selected. Age had a significant effect on coordinate b* (p = 0.000). CONCLUSIONS: Statistically significant differences were found between the L*, a* and b* color coordinates of the attached gingiva between men and women, although the color difference is below the clinical acceptance threshold. The attached gingiva takes on a bluish color as the patients get older, which means that the b* coordinate decreases. CLINICAL SIGNIFICANCE: With a prosthodontic approach, knowledge of the CIELAB natural attached gingival coordinates based on the patient's age and gender will facilitate the clinician's work in selecting the appropriate color. The CIELAB system values found can be used as a gingival shade guide reference.

Evaluation of native canine skin color by smartphone-based dermatoscopy.

BACKGROUND: Human skin color, predominantly determined by the chromophores of melanin, hemoglobin, and exogenous carotenoids, is often measured to serve various medical and cosmetic applications. Although colorimetry has been used to evaluate the skin erythema in allergic dogs, the native canine skin color remains unknown. METHODS: We measured the skin color in 101 healthy dogs using a calibrated optical system with a smartphone and a mobile dermatoscope DermLite DL1. The results were retrieved in the CIELAB color system and compared to the human color ranges. RESULTS: The lightness (L*) of canine skin ranged from 28.5 to 78.3, which is slightly broader than that of human skin. There was a difference of 3.9 in redness (a*) between canine and human skin, but this variation could be attributed to the similarly valued colorimetric error of the optical system. Nonetheless, the skin yellowness was significantly different for dogs and humans (respective median b* of 12.3 versus 16.6, p < 0.01). This difference might be due to canids not being able to accumulate typically yellowish carotenoids. Furthermore, the native canine skin color did not exhibit a typical dependence between the coordinates of lightness (L*) and yellowness (b*), known as the individual typology angle, °ITA. CONCLUSION: We reported the first dataset of the native canine skin color in the CIELAB color space. We discovered a similarity in skin lightness and a difference in skin yellowness. However, further studies are needed for a more precise comparison of skin redness.

An approach to improve the baking properties and determine the onset of browning in fat-free mozzarella cheese.

Compared with low-moisture part-skim mozzarella and mozzarella cheese, bake performance of low-fat and fat-free mozzarella on pizza has a lot to desire. We hypothesized that a water-soaking pretreatment step of low-fat and fat-free cheese shreds before baking would improve pizza baking performance. The study also examined the correlation of the onset of cheese browning with the rate of moisture loss, changes in cheese surface temperature, and 3-dimensional (3D) plot L* a* b* CIELAB color analysis. The pretreatment of soaking cheese shreds in water improved the baking properties of fat-free mozzarella cheese on pizza. Compared with the control sample, which demonstrated significant shred identity, poor shred melt, fusion, and stretch during a pizza bake with fat-free mozzarella, the soaked cheese (SC) sample demonstrated satisfactory cheese melt, fusion, and stretch. In addition, the SC sample had desired browning as opposed to the control sample's excessive browning. The additional moisture from the soaking pretreatment aided in delaying the onset of cheese browning in the SC sample when compared with the control sample. For both the control and SC samples, there was a strong correlation between the onset of cheese browning with the peak of moisture-loss rate, and an increase in cheese surface temperature (>100°C). The color analysis of the 3D plot confirmed the relationship between the onset of cheese browning and the shift in L* (lightness), a* (red-green color), and b* (blue-yellow) values. According to the study's findings, soaking cheese shreds before baking can help improve bake performance on pizza. Furthermore, 3 measurement tools used in the study, (1) moisture-loss rate, (2) cheese surface temperature, and (3) 3D plot CIELAB color, were useful in determining the onset of cheese browning and can be applied to different intervention strategies to control cheese browning during pizza baking.

A Novel Method for Calibration of Digital Soil Images Captured under Irregular Lighting Conditions.

Soil color is commonly used as an indicator to classify soil and identify its properties. However, color-based soil assessments are susceptible to variations in light conditions and the subjectivity of visual evaluations. This study proposes a novel method of calibrating digital images of soil, regardless of lighting conditions, to ensure accurate identification. Two different color space models, RGB and CIELAB, were assessed in terms of their potential utility in calibrating changes to soil color in digital images. The latter system was determined to be suitable, as a result of its ability to accurately reflect illuminance and color temperature. Linear regression equations relating soil color and light conditions were developed based on digital images of four different types of soil samples, each photographed under 15 different light conditions. The proposed method can be applied to calibrate variations in the soil color obtained by digital images, thus allowing for more standardized, objective, and accurate classification and evaluation of soil based on its color.

Capability of the TrueColor Sensor Array for Determining the Nitrogen Supply in Winter Barley (Hordeum vulgare L.).

In agriculture, efforts are being made to reduce pesticides and fertilizers because of the possible negative environmental impacts, high costs, political requirements, and declining social acceptance. With precision farming, significant savings can be achieved by the site-specific application of fertilizers. In contrast to currently available single sensors and camera-based systems, arrays or line sensors provide a suitable spatial resolution without requiring complex signal processing and promise significant potential regarding price and precision. Such systems comprise a cost-effective and compact unit that can be extended to any working width by cascading into arrays. In this study, experiments were performed to evaluate the applicability of a TrueColor sensor array in monitoring the nitrogen supply of winter barley during its growth. This sensor is based on recording the reflectance values in various channels of the CIELab color space: luminosity, green-red, and blue-yellow. The unique selling point of this sensor is the detection of luminosity because only the CIELab color space provides this opportunity. Strong correlations were found between the different reflection channels and the nitrogen level (R² = 0.959), plant coverage (R² = 0.907), and fresh mass yield (R² = 0.866). The fast signal processing allows this sensor to meet stringent demands for the operating speed, spatial resolution, and price structure.

Effect of UV-C Irradiation on the Shelf Life of Fresh-Cut Potato and Its Sensory Properties after Cooking.

Research background: Potato tissue is damaged during fresh-cut production, which makes fresh-cut potato susceptible to the quality loss and microbiological spoilage. At the same time, such products are desirable due to their convenience; however, they are extremely sensitive and have short shelf life. The main challenge of the fresh-cut potato industry is to find possibilities to overcome these drawbacks. UV-C treatment, known for its antibacterial activity, is a promising technique and it shows a potential to improve shelf life of fresh-cut potato products. Experimental approach: The influence of the UV-C treatment on the safety and quality, as well as sensory traits of fresh-cut potato (Solanum tuberosum L. cv. Birgit) during storage was examined. For this purpose, 0-, 3-, 5- and 10-min UV-C irradiation was applied on vacuum-packed potato slices pretreated with sodium ascorbate solution. During 23 days of storage at (6±1) °C, microbiological, physicochemical and sensory properties of raw samples were monitored, along with sensory properties of boiled and fried fresh-cut potatoes. Results and conclusions: The 5- and 10-min UV-C treatments significantly reduced microbial growth, increased total solids and lightness (L*), and positively affected odour and firmness of raw potatoes. Cooked UV-C-treated samples were described with more pronounced characteristic potato odour and taste. Overall, UV-C-treated fresh-cut potato retained its good quality and sensory traits up to 15 days at (6±1) °C. Novelty and scientific contribution: To the best of our knowledge, this is the first scientific article dealing with the effect of UV-C light on durability (safety, quality and sensory traits) of fresh-cut potato cv. Birgit and its suitability for boiling and frying. In general, UV-C treatment is a known antimicrobial technique, but its application on fresh-cut potato is poorly explored. Results confirmed that vacuum-packed fresh-cut potato treated only with UV-C and sodium ascorbate as anti-browning agent, without the addition of chemical preservatives, had twofold longer shelf-life at (6±1) °C than the fresh-cut potato not treated with UV-C. Fresh-cut potato treated with UV-C retained good overall quality and sensory properties either raw, boiled or fried. Results of this study could also be useful for producers in terms of potential UV-C application as a strategy for prolonging the shelf-life of fresh-cut potato.

3D Master Toothguide Is Adequate to Subjective Shade Selection?

Background and Objectives: To study the validity and the degree of representability of the toothguide 3D Master, with 26 physically shade tabs, on the natural tooth colour on a sample of the Spanish population. Materials and Methods: Natural tooth colour was measured in a sample of 1361 Spanish participants of both genders distributed within an age range of 18 and 89 years of age. The colour coordinates were calculated and the frequency of the 26 physically shade tabs of the toothguide as well as the "intermediate shades" (without physical representation in toothguide) through the Easyshade Compact (Vita-Zahnfabrik) spectrophotometer using the 3D Master System nomenclature. The colour differences between the "intermediate shades" were calculated using the Euclidean formula (ΔEab*). The program used for the present descriptive statistical analysis of the results was SAS 9.1.3. Results: A total of 49 "intermediate shades" were registered in 816 participants (60%). The colour coordinates of the 49 'intermediate shades' cover colour coordinates ranging from 0M1.5 (L* 100.0, C* 7.70, h* 112.2) to 5M2.5 (L* 56.8, C* 35.8, h* 78.5). Not all possible 3D Master System's "intermediate shades" were registered in the population studied. 82.4% of the colour differences among the "intermediate shades" were clinically unacceptable (ΔEab* ≥ 5.5 units). Conclusions: Only 40% of the population studied presented a natural tooth colour belonging to the 3D Master Toothguide's physical shade tabs.

Metabolome and transcriptome profiling reveals anthocyanin contents and anthocyanin-related genes of chimeric leaves in Ananas comosus var. bracteatus.

BACKGROUND: Ananas comosus var. bracteatus is a colorful plant used as a cut flower or landscape ornamental. The unique foliage color of this plant includes both green and red leaves and, as a trait of interest, deserves investigation. In order to explore the pigments behind the red section of the chimeric leaves, the green and red parts of chimeric leaves of Ananas comosus var. bracteatus were sampled and analyzed at phenotypic, cellular and molecular levels in this study. RESULTS: The CIELAB results indicated that the a* values and L* values samples had significant differences between two parts. Freehand sections showed that anthocyanin presented limited accumulation in the green leaf tissues but obviously accumulation in the epidermal cells of red tissues. Transcriptomic and metabolomic analyses were performed by RNA-seq and LC-ESI-MS/MS. Among the 508 identified metabolites, 10 kinds of anthocyanins were detected, with 6 significantly different between the two samples. The cyanidin-3,5-O-diglucoside content that accounts for nearly 95.6% in red samples was significantly higher than green samples. RNA-Seq analyses showed that 11 out of 40 anthocyanin-related genes were differentially expressed between the green and red samples. Transcriptome and metabolome correlations were determined by nine quadrant analyses, and 9 anthocyanin-related genes, including MYB5 and MYB82, were correlated with 7 anthocyanin-related metabolites in the third quadrant in which genes and metabolites showing consistent change. Particularly, the PCCs between these two MYB genes and cyanidin-3,5-O-diglucoside were above 0.95. CONCLUSION: Phenotypic colors are closely related to the tissue structures of different leaf parts of Ananas comosus var. bracteatus, and two MYB transcription factors might contribute to differences of anthocyanin accumulation in two parts of Ananas comosus var. bracteatus chimeric leaves. This study lay a foundation for further researches on functions of MYBs in Ananas comosus var. bracteatus and provides new insights to anthocyanin accumulation in different parts of chimeric leaves.

Genome-wide association mapping reveals new loci associated with light-colored seed coat at harvest and slow darkening in carioca beans.

BACKGROUND: Common bean (Phaseolus vulgaris L.) is a legume whose grain can be stored for months, a common practice among Brazilian growers. Over time, seed coats become darker and harder to cook, traits that are undesirable to consumers, who associate darker-colored beans with greater age. Like commercial pinto and cranberry bean varieties, carioca beans that have darker seeds at harvest time and after storage are subject to decreased market values. RESULTS: The goal of our study was to identify the genetic control associated with lightness of seed coat color at harvest (HL) and with tolerance to post-harvest seed coat darkening (PHD) by a genome-wide association study. For that purpose, a carioca diversity panel previously validated for association mapping studies was used with 138 genotypes and 1,516 high-quality SNPs. The panel was evaluated in two environments using a colorimeter and the CIELAB scale. Shelf storage for 30 days had the most expressive results and the L* (luminosity) parameter led to the greatest discrimination of genotypes. Three QTL were identified for HL, two on chromosome Pv04 and one on Pv10. Regarding PHD, results showed that genetic control differs for L* after 30 days and for the ΔL* (final L*-initial L*); only ΔL* was able to properly express the PHD trait. Four phenotypic classes were proposed, and five QTL were identified through six significant SNPs. CONCLUSIONS: Lightness of seed coat color at harvest showed an oligogenic inheritance corroborated by moderate broad-sense heritability and high genotypic correlation among the experiments. Only three QTL were significant for this trait - two were mapped on Pv04 and one on Pv10. Considering the ΔL, six QTL were mapped on four different chromosomes for PHD. The same HL QTL at the beginning of Pv10 was also associated with ΔL* and could be used as a tool in marker-assisted selection. Several candidate genes were identified and may be useful to accelerate the genetic breeding process.

Low-Illumination Image Enhancement in the Space Environment Based on the DC-WGAN Algorithm.

Owing to insufficient illumination of the space station, the image information collected by the intelligent robot will be degraded, and it will not be able to accurately identify the tools required for the robot's on-orbit maintenance. This situation increases the difficulty of the robot's maintenance in a low-illumination environment. We proposes a novel enhancement method for images under low-illumination, namely, a deep learning algorithm based on the combination of deep convolutional and Wasserstein generative adversarial networks (DC-WGAN) in CIELAB color space. The original low-illuminance image is converted from the RGB space to the CIELAB color space which is relatively close to human vision, to accurately estimate the illumination image, and effectively reduce the effect of uneven illumination. DC-WGAN is applied to enhance the brightness component by increasing the width of the generation network to obtain more image features. Subsequently, the LAB is converted into RGB space to obtain the final enhanced image. The feasibility of the algorithm is verified by experiments on low-illuminance image under general, special, and actual conditions and comparing the experimental results with four commonly used algorithms. This study lays a technical foundation for robot target recognition and on-orbit maintenance in a space environment.

Analysis of color and fluorescence of different dentin ceramics and natural human dentin's to enhance shade matching.

OBJECTIVES: This study aimed to compare the color and fluorescence of five dentin ceramic systems with human dentin to enhance shade matching. MATERIALS AND METHODS: Five dentin ceramic systems, including Vita, GC, Creation, Noritake, and Ivoclar in 16 shades, and 12 human dentins, were prepared. The fluorescence and color coordinates were measured at 380-780 nm. A light source including a visible and a ultraviolet (UV) complemented with a geometry 45° illumination and 0° observer was used. The mean minimum difference in color coordinates and fluorescence efficiency was statistically analyzed before and after UV excitation by one-way ANOVA (p < 0.05). Post-hoc multiple comparisons were performed using the LSD test (p < 0.05). RESULTS: While the fluorescence efficiency of Noritake and Ivoclar ceramics displayed no significant difference with natural dentin, Vita, GC, and Creation ceramics presented a lower fluorescence compared to the natural dentin (p < 0.05). Noritake and Ivoclar had a better color match with human dentin than the other ceramics. Studying the color differences under UV showed that just Creation offers statistically worse performance than the other ceramic. CONCLUSIONS: The color and fluorescence of the used different brands of dentin ceramic are not the same as the human dentin. However, Noritake and Ivoclar presented the most similarity. CLINICAL SIGNIFICANCE: Knowledge of ceramic and natural teeth fluorescence is essential for acceptable reproduction of optical properties in esthetic restorations.

Impact of resin composite cement on color of computer-aided design/computer-aided manufacturing ceramics.

OBJECTIVE: To analyze the impact of the color of a resin composite cement (RCC) on the optical properties of different computer-aided design and computer-aided manufacturing (CAD/CAM) ceramics. MATERIALS AND METHODS: Specimens (N = 220, thickness: 0.9 ± 0.03 mm) were fabricated from: leucite (Initial LRF Block/IPS Empress CAD), lithium disilicate (Amber Mill/IPS e.max CAD), lithium metasilicate (Celtra Duo), and lithium alumina silicate ceramic (n!ce) in translucency levels HT and LT. All specimens were bonded with an RCC (Light+/Warm+). Color was analyzed (spectrophotometer) initially as well as after bonding of RCC with CAD/CAM ceramics using CIELab and CIEDE2000. Kolmogorov-Smirnov test, one-way ANOVA and t test served for analyzing (α = 0.05). RESULTS: Highest impact on ΔE presented the choice of ceramic (ηp2  = 0.155/p < 0.001), followed by translucency level (HT/LT; ηp2  = 0.050/p = 0.001) as well as interaction between ceramic and translucency level (ηp2  = 0.175/p < 0.001). ΔE00 was mainly influenced by the choice of ceramic (ηp2  = 0.490/p < 0.001), the shade of resin composite (ηp2  = 0.031/p = 0.012) as well as the interaction between ceramic and resin composite (ηp2  = 0.258/p < 0.001). CONCLUSIONS: RCC shades presented differential impacts on color change of CAD/CAM ceramics. CLINICAL SIGNIFICANCE: Knowledge of the impact of available RCC shades on different CAD/CAM ceramics is crucial for an esthetic outcome and proper selection of ceramic restorations.

The Influence of Temperature, Storage Conditions, pH, and Ionic Strength on the Antioxidant Activity and Color Parameters of Rowan Berry Extracts.

Recent trends in the food industry combined with novel methods in agriculture could transform rowan into a valuable raw material with potential technological applications. Thus, the aim of this research was to investigate the content of bioactive compounds in its fruits and to assess the color and antioxidant stability of the extracts prepared from such fruits during various thermal treatments and at different pH and ionic strength values. Various spectrophotometric methods, HPLC, and capillary electrophoresis were used to quantify the concentrations of bioactive compounds-polyphenols, carotenoids, organic acids, and to assess antioxidant activity and color. The results show that rowan berries contain circa 1.34-1.47 g/100 g of polyphenols among which include catechin, epicatechin, ferulic acid methyl ester, procyanidin B1, etc.; ca 21.65 mg/100 g of carotenoids including zeaxanthin, ß-cryptoxanthin, all-trans-ß-carotene, and various organic acids such as malic, citric, and succinic, which result in a high antioxidant activity of 5.8 mmol TE/100 g. Results also showed that antioxidant activity exhibited high stability when the extract was subjected to various thermal treatments, pHs, and ionic strengths, while color was mainly impacted negatively when a temperature of 100 °C was employed. This data confirms the technological potential of this traditional, yet often overlooked species.

Innovative Development of Batch Dyed 3D Printed Acrylonitrile/Butadiene/Styrene Objects.

According to the great impact of additive technology on the development of modern industry, a lot of research is being done to obtain 3D printed parts with better properties. This research is extremely important because there are no scientific papers in the field of post dyeing of acrylonitrile/butadiene/styrene (ABS) 3D printed parts. The experiment was carried out using disperse dyes on ABS specimens. The obtained coloration of the specimens was in the primary colors (yellow, red, and blue) in the specified dyestuff concentration range and was evaluated using an objective CIELab system. Based on the obtained color parameters, remission values and Kubelka-Munk coefficient, dye mixtures and an ombre effect were performed to obtain patterns in the desired hues. Abrasion resistance of disperse dyed specimens was tested using different abrasive materials over a wide range of fineness to simulate different indoor and outdoor soils and was compared to abrasion resistance of specimens produced from the industrially dyed wire with the master batch. The results show that 3D printed ABS products can be produced in one or more desired shades with satisfactory abrasion resistance. This undoubtedly represents the added value of 3D printed ABS parts and extends their application to the field of creative industries and design, specifically footwear design.