Differential modulation of the lipoxygenase cascade during typical and latent Pectobacterium atrosepticum infections.

BACKGROUND AND AIMS: Plant diseases caused by Pectobacterium atrosepticum are often accompanied by extensive rot symptoms. In addition, these bacteria are able to interact with host plants without causing disease for long periods, even throughout several host plant generations. There is, to date, no information on the comparative physiology/biochemistry of symptomatic and asymptomatic plant-P. atrosepticum interactions. Typical (symptomatic) P. atrosepticum infections are associated with the induction of plant responses mediated by jasmonates, which are one of the products of the lipoxygenase cascade that gives origin to many other oxylipins with physiological activities. In this study, we compared the functioning of the lipoxygenase cascade following typical and latent (asymptomatic) infections to gain better insight into the physiological basis of the asymptomatic and antagonistic coexistence of plants and pectobacteria. METHODS: Tobacco plants were mock-inoculated (control) or infected with the wild type P. atrosepticum (typical infection) or its coronafacic acid-deficient mutant (latent infection). The expression levels of the target lipoxygenase cascade-related genes were assessed by Illumina RNA sequencing. Oxylipin profiles were analysed by GC-MS. With the aim of revising the incorrect annotation of one of the target genes, its open reading frame was cloned to obtain the recombinant protein, which was further purified and characterized using biochemical approaches. KEY RESULTS: The obtained data demonstrate that when compared to the typical infection, latent asymptomatic P. atrosepticum infection is associated with (and possibly maintained due to) decreased levels of 9-lipoxygenase branch products and jasmonic acid and increased level of cis-12-oxo-10,15-phytodienoic acid. The formation of 9-oxononanoic acid and epoxyalcohols in tobacco plants was based on the identification of the first tobacco hydroperoxide lyase (HPL) with additional epoxyalcohol synthase (EAS) activity. CONCLUSIONS: Our results contribute to the hypothesis of the oxylipin signature, indicating that different types of plant interactions with a particular pathogen are characterized by the different oxylipin profiles of the host plant. In addition, the tobacco LOC107825278 gene was demonstrated to encode an NtHPL (CYP74C43) enzyme yielding volatile aldehydes and aldoacids (HPL products) as well as oxiranyl carbinols (EAS products).

Rotting Grapes Don't Improve with Age: Cluster Rot Disease Complexes, Management, and Future Prospects.

Cluster rots can be devastating to grape production around the world. There are several late-season rots that can affect grape berries, including Botrytis bunch rot, sour rot, black rot, Phomopsis fruit rot, bitter rot, and ripe rot. Tight-clustered varieties such as 'Pinot gris', 'Pinot noir', and 'Vignoles' are particularly susceptible to cluster rots. Symptoms or signs for these rots range from discolored berries or gray-brown sporulation in Botrytis bunch rot to sour rot, which smells distinctly of vinegar due to the presence of acetic acid bacteria. This review discusses the common symptoms and disease cycles of these different cluster rots. It also includes useful updates on disease diagnostics and management practices, including cultural practices in commercial vineyards and future prospects for disease management. By understanding what drives the development of different cluster rots, researchers will be able to identify new avenues for research to control these critical pathogens.

The Role of ABA in the Interaction between Citrus Fruit and Penicillium digitatum.

Abscisic acid (ABA) protects citrus fruit against Penicillium digitatum infection. The global mechanisms involved in the role of ABA in the P. digitatum-citrus fruit interaction are unknown. Here, we determine the transcriptome differences between the Navelate (Citrus sinensis (L.) Osbeck) orange and its ABA-deficient mutant Pinalate, which is less resistant to infection. Low ABA levels may affect both the constitutive mechanisms that protect citrus fruit against P. digitatum and early responses to infection. The repression of terpenoid, phenylpropanoid and glutation metabolism; of oxidation-reduction processes; and of processes related to the defense response to fungus and plant hormone signal transduction may be one part of the constitutive defense reduced in the mutant against P. digitatum. Our results also provide potential targets for developing P. digitatum-citrus fruit-resistant varieties. Of those up-regulated by ABA, a thaumatin protein and a bifunctional inhibitor/LTP, which are relevant in plant immunity, were particularly remarkable. It is also worth highlighting chlorophyllase 1 (CLH1), induced by infection in Pinalate, and the OXS3 gene, which was down-regulated by ABA, because the absence of OXS3 activates ABA-responsive genes in plants.

Diversity, Pathogenicity, and Fungicide Sensitivity of Fungal Species Associated with Late-Season Rots of Wine Grape in the Mid-Atlantic United States.

Late-season bunch rots cause major losses in grape production every year in the Mid-Atlantic United States, but the causal agents are not well characterized. In this study, 265 fungal isolates were collected from rotten grapes from 2014 to 2020 and identified to the genus level according to internal transcribed spacer sequences. The most prevalent of the 15 genera were Botrytis, Colletotrichum, Aspergillus, Alternaria, Pestalotiopsis, and Neopestalotiopsis. Of these, isolates within three prevalent, yet understudied, genera were identified to be Aspergillus uvarum, Alternaria alternata, and Neopestalotiopsis rosae. The pathogenicity of these three fungal species was evaluated in two field trials by artificially inoculating wounded and nonwounded grapes (Vitis vinifera) of four cultivars at the phenological stages of bloom, véraison, and preharvest. Upon ripening, fruit were weighed and assessed for severity of multiple diseases. On nonwounded fruit, A. uvarum caused significantly higher disease severity than the control in both seasons. On wounded fruit, each inocula caused significantly higher disease than the respective controls in the first season, but only A. uvarum and Botrytis cinerea caused this in the second season. Also, wounding was found to have a detrimental effect on cluster weight, which was significantly influenced by inoculation timing and cultivar. Lastly, A. uvarum and N. rosae were tested for sensitivity to azoxystrobin, boscalid, and difenoconazole. The A. uvarum isolates were found to be more sensitive to boscalid and difenoconazole in general, with varying sensitivity to azoxystrobin. N. rosae isolates were resistant to boscalid and azoxystrobin but displayed much higher sensitivity to difenoconazole. Evidence from the isolate collection and field trials demonstrates that A. uvarum could be a significant pathogen of wine grapes in the Mid-Atlantic United States. Results from this study will be useful for the identification and management of the understudied Alternaria, Aspergillus, and Neopestalotiopsis fruit rots of wine grapes.

How the Global Tea Industry Copes With Fungal Diseases - Challenges and Opportunities.

Tea (Camellia sinensis [L.] O. Kuntze) is a plantation crop, grown commercially in Asia, Africa, and South America. Among biotic threats to tea production, diseases caused by fungal pathogens are most significant. Worldwide, tea plants are challenged by several root, stem, and foliar diseases. Foliar diseases, blister blight, gray blight, and brown blight are particularly important as they adversely affect the bud and the two youngest leaves, causing loss of harvestable shoots. Over the past several decades, climate change and field management practices have influenced the risk of crop damage by several fungal pathogens, as well as the development and spread of diseases. Management interventions, such as the adoption of good cultural/agronomic practices, use of fungicides and microbial biocontrol agents, plant defense elicitors, and deployment of resistant cultivars, have mitigated damage to tea plants caused by fungal diseases. A clearer understanding of knowledge gaps and the benefits of plant disease management strategies available is needed. The present article reviews the prevailing knowledge of major fungal pathogens of the tea crop, their genetic variability, the damage they cause and its economic impact, and the need for new disease management strategies as climate change intensifies. We will also emphasize important knowledge gaps that are priority targets for future research.

Antifungal activity of propyl disulphide from neem against Lasiodiplodia theobromae and Neofusicoccum parvum causing stem end rot in mango.

AIM: To investigate the antifungal effect of the neem extract volatile propyl disulphide against the fungal plant pathogens. METHODS AND RESULTS: Postharvest pathogens were isolated and identified as Lasiodiplodia theobromae and Neofusicoccum parvum causing the stem end rot of mango fruit. Antifungal activity of propyl disulphide at different concentrations was determined in comparison with ethanol and distilled water. Both diffusion assays were effective in controlling the mycelial growth of the tested fungi. The high coefficient of determination (R2 , 0·970-0·997) of the growth curves indicated the good fit of the experimental data to the Gompertz growth model. Propyl disulphide significantly (P ≤ 0·05) decreased the mycelial growth during the stationary phase (A) and increased the lag phase (λ) than both control (ethanol and distilled water). CONCLUSION: This compound can potentially be applied to other tropical and subtropical fruits for the successful control of postharvest losses of fresh produce. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of propyl disulphide from neem extract against plant pathogens and could be beneficial for effective microbial decay prevention in food industries.

Volatile organic compounds from Hypoxylon anthochroum endophytic strains as postharvest mycofumigation alternative for cherry tomatoes.

The antifungal activity and chemical composition of the volatile organic compounds (VOCs) produced by four Hypoxylon anthochroum endophytic strains were analyzed. The bioactivity of the VOCs synthesized at different periods of incubation on rice medium was assessed, both in vivo and in vitro, against the phytopathogen Fusarium oxysporum. The in vivo effect was evaluated on cherry tomatoes, while the mechanism of action was determined in vitro analyzing the phytopathogen's growth, respiration and cell membrane permeability. In general, the VOCs from all strains and incubation periods significantly inhibited the growth of F. oxysporum on cherry tomatoes with percentages over 50%. They significantly inhibited the pathogen growth and respiration, and altered the cell membrane permeability and hyphal morphology. The chemical composition was analyzed after solid phase microextraction. In total, 36 VOCs were identified in the four strains, mainly sesquiterpenes and monoterpenes. Among the monoterpenes, eucalyptol had the highest fiber affinity (>60% area) in three of the four strains studied; thus, it could be considered as a chemical marker for H. antochroum. Chemical markers are important for the identification and differentiation of species. The H. anthochroum strains are potential mycofumigation agents against postharvest diseases caused by F. oxysporum.

Development of a powder formulation based on Bacillus cereus sensu lato strain B25 spores for biological control of Fusarium verticillioides in maize plants.

Maize is an economically important crop in northern Mexico. Different fungi cause ear and root rot in maize, including Fusarium verticillioides (Sacc.) Nirenberg. Crop management of this pathogen with chemical fungicides has been difficult. By contrast, the recent use of novel biocontrol strategies, such as seed bacterization with Bacillus cereus sensu lato strain B25, has been effective in field trials. These approaches are not without their problems, since insufficient formulation technology, between other factors, can limit success of biocontrol agents. In response to these drawbacks, we have developed a powder formulation based on Bacillus B25 spores and evaluated some of its characteristics, including shelf life and efficacy against F. verticillioides, in vitro and in maize plants. A talc-based powder formulation containing 1 × 10(9) c.f.u. g(-1) was obtained and evaluated for seed adherence ability, seed germination effect, shelf life and antagonism against F. verticillioides in in vitro and in planta assays. Seed adherence of viable bacterial spores ranged from 1.0 to 1.41 × 10(7) c.f.u. g(-1). Bacteria did not display negative effects on seed germination. Spore viability for the powder formulation slowly decreased over time, and was 53 % after 360 days of storage at room temperature. This formulation was capable of controlling F. verticillioides in greenhouse assays, as well as eight other maize phytopathogenic fungi in vitro. The results suggest that a talc-based powder formulation of Bacillus B25 spores may be sufficient to produce inoculum for biocontrol of maize ear and root rots caused by F. verticillioides.

Optimization of Statistical Methods Impact on Quantitative Proteomics Data.

As tools for quantitative label-free mass spectrometry (MS) rapidly develop, a consensus about the best practices is not apparent. In the work described here we compared popular statistical methods for detecting differential protein expression from quantitative MS data using both controlled experiments with known quantitative differences for specific proteins used as standards as well as "real" experiments where differences in protein abundance are not known a priori. Our results suggest that data-driven reproducibility-optimization can consistently produce reliable differential expression rankings for label-free proteome tools and are straightforward in their application.

Use of Pseudomonas protegens to Control Root Rot Disease Caused by Boeremia exigua var. exigua in Industrial Chicory (Cichorium intybus var. sativum Bisch.).

Boeremia exigua var. exigua is a recurrent pathogen causing root rot in industrial chicory. Currently, there is no chemical or varietal control for this disease, and thus, management strategies need to be developed. This study determined the biocontrol effect of strains of Pseudomonas protegens bacteria with antimicrobial compounds on the fungus B. exigua var. exigua under in vitro, in vivo, and field conditions. In addition, root colonization by these bacteria was estimated by the phlD-specific PCR-based dilution end point assay. Eighteen isolates of Pseudomonas spp were evaluated, and the strains that showed the greatest in vitro inhibition of fungal mycelial growth (mm), Ca10A and ChB7, were selected. Inoculation with the strain ChB7 showed less severity (necrotic area) under in vivo conditions (root trials) compared with the control inoculated with the pathogen (p ≤ 0.05). The molecular analysis revealed that the root colonization of plants grown in pots was equal to or greater than 70%. Similar levels were observed in the field trials conducted at the Selva Negra and Canteras experimental stations (2015-2016 season), with values ranging from 85.7 to 70.5% and from 75.0 to 79.5%, respectively. Regarding yield (ton ha-1), values were higher in the treatments inoculated with strains Ca10A and ChB7 (p ≤ 0.05) at both experimental sites, while a lower incidence and severity of root rot were observed at Selva Negra. These results suggest that the Chilean strains of P. protegens are a promising tool for the control of root diseases in industrial chicory.

Integrated Management of Pathogens and Microbes in Cannabis sativa L. (Cannabis) under Greenhouse Conditions.

The increased cultivation of high THC-containing Cannabis sativa L. (cannabis), particularly in greenhouses, has resulted in a greater incidence of diseases and molds that can negatively affect the growth and quality of the crop. Among them, the most important diseases are root rots (Fusarium and Pythium spp.), bud rot (Botrytis cinerea), powdery mildew (Golovinomyces ambrosiae), cannabis stunt disease (caused by hop latent viroid), and a range of microbes that reduce post-harvest quality. An integrated management approach to reduce the impact of these diseases/microbes requires combining different approaches that target the reproduction, spread, and survival of the associated pathogens, many of which can occur on the same plant simultaneously. These approaches will be discussed in the context of developing an integrated plan to manage the important pathogens of greenhouse-grown cannabis at different stages of plant development. These stages include the maintenance of stock plants, propagation through cuttings, vegetative growth of plants, and flowering. The cultivation of cannabis genotypes with tolerance or resistance to various pathogens is a very important approach, as well as the maintenance of pathogen-free stock plants. When combined with cultural approaches (sanitation, management of irrigation, and monitoring for diseases) and environmental approaches (greenhouse climate modification), a significant reduction in pathogen development and spread can be achieved. The use of preventive applications of microbial biological control agents and reduced-risk biorational products can also reduce disease development at all stages of production in jurisdictions where they are registered for use. The combined use of promising strategies for integrated disease management in cannabis plants during greenhouse production will be reviewed. Future areas for research are identified.

Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum.

We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1-5) that are non-overlapping and linearly arranged. The largest, ORF5, encodes a large polypeptide L containing a conserved regions corresponding to Mononegavirales RNA-dependent RNA polymerase and mRNA-capping enzyme region V; the putative functions of the remaining four ORFs are unknown. The L protein encoded by ORF5 shared a high amino acid identity of 65% with that of Hubei rhabdo-like virus 4, a mymonavirus that associated with arthropods. However, the L protein of FoMyV1 also showed amino acid similarity (27-36%) with proteins of mynonaviruses that infect the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cineaea. Phylogenetic analysis based on L protein showed that FoMyV1 is clustered with the members of the genus Hubramonavirus in the family Mymonaviridae. Moreover, we found that FoMyV1 could successfully transfer by hyphal anastomosis to a virus-free strain. FoMyV1 reduced the vegetative growth and conidium production of its fungal host but did not alter its virulence. To the best of our knowledge, this is not only the first mymonavirus described in the species F. oxysporum, but also the first Hubramonavirus species found to infect a fungus. However, the incidence of FoMyV1 infections in the tested F. oxysporum strains was only 1%.

Potential Role of Biocontrol Agents for Sustainable Management of Fungal Pathogens Causing Canker and Fruit Rot of Pistachio in Italy.

Pistachio (Pistacia vera) is an important Mediterranean crop. In Italy, pistachio is cultivated in the southern regions, of which Sicily is the main production area. Recently, the phytopathological situation of this crop has started to be updated, and new diseases have been discovered, studied, and reported. Botryosphaeriaceae spp. and Leptosillia pistaciae are major canker/rot pathogens, and Cytospora pistaciae and Eutypa lata have been reported as minor canker pathogens. In this paper, we evaluated different biological control agents, belonging to Trichodermaasperellum, T. atroviride and T. harzianum, as well as some Bacillus amyloliquefaciens strains, against above-mentioned pathogens. Results of dual culture assays showed that all the biological products, both fungi and bacteria, were able to inhibit the mycelial growth of the pathogens in vitro. Experiments using detached twigs showed no effect of biocontrol agents in reducing infections, except for Neofusicoccum hellenicum treated with T. harzianum T22 and Leptosillia pistaciae treated with B. amyloliquefaciens D747. Results of detached fruit experiments showed an efficacy ranging from 32.5 to 66.9% of all the biological products in reducing the lesions caused by N. mediterraneum. This study provides basic information for future research on biological control of pistachio diseases and future prospects for search of more effective biological control agents for canker diseases than those studied here.

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize.

Fusarium (FER) and Gibberella ear rots (GER) are the two most devastating diseases of maize (Zea mays L.) which reduce yield and affect grain quality worldwide, especially by contamination with mycotoxins. Genetic improvement of host resistance to effectively tackle FER and GER diseases requires the identification of stable quantitative trait loci (QTL) to facilitate the application of genomics-assisted breeding for improving selection efficiency in breeding programs. We applied improved meta-analysis algorithms to re-analyze 224 QTL identified in 15 studies based on dense genome-wide single nucleotide polymorphisms (SNP) in order to identify meta-QTL (MQTL) and colocalized genomic loci for fumonisin (FUM) and deoxynivalenol (DON) accumulation, silk (SR) and kernel (KR) resistances of both FER and GER, kernel dry-down rate (KDD) and husk coverage (HC). A high-resolution genetic consensus map with 36,243 loci was constructed and enabled the projection of 164 of the 224 collected QTL. Candidate genes (CG) mining was performed within the most refined MQTL, and identified CG were cross-validated using publicly available transcriptomic data of maize under Fusarium graminearum infection. The meta-analysis revealed 40 MQTL, of which 29 were associated each with 2-5 FER- and/or GER-related traits. Twenty-eight of the 40 MQTL were common to both FER and GER resistances and 19 MQTL were common to silk and kernel resistances. Fourteen most refined MQTL on chromosomes 1, 2, 3, 4, 7 and 9 harbored a total of 2,272 CG. Cross-validation identified 59 of these CG as responsive to FER and/or GER diseases. MQTL ZmMQTL2.2, ZmMQTL9.2 and ZmMQTL9.4 harbored promising resistance genes, of which GRMZM2G011151 and GRMZM2G093092 were specific to the resistant line for both diseases and encoded "terpene synthase21 (tps21)" and "flavonoid O-methyltransferase2 (fomt2)", respectively. Our findings revealed stable refined MQTL harboring promising candidate genes for use in breeding programs for improving FER and GER resistances with reduced mycotoxin accumulation. These candidate genes can be transferred into elite cultivars by integrating refined MQTL into genomics-assisted backcross breeding strategies.

Updating the Methodology of Identifying Maize Hybrids Resistant to Ear Rot Pathogens and Their Toxins-Artificial Inoculation Tests for Kernel Resistance to Fusarium graminearum, F. verticillioides, and Aspergillus flavus.

Resistance to toxigenic fungi and their toxins in maize is a highly important research topic, as mean global losses are estimated at about 10% of the yield. Resistance and toxin data of the hybrids are mostly not given, so farmers are not informed about the food safety risks of their grown hybrids. According to the findings aflatoxin regularly occurs at preharvest in Hungary and possibly other countries in the region can be jeopardized. We tested, with an improved methodology (two isolates, three pathogens, and a toxin control), 18 commercial hybrids (2017-2020) for kernel resistance (%), and for toxin contamination separately by two-two isolates of F. graminearum, F. verticillioides (mg/kg), and A. flavus (µg/kg). The preharvest toxin contamination was measured in the controls. Highly significant kernel resistance and toxin content differences were identified between hybrids to the different fungi. Extreme high toxin production was found for each toxic species. Only about 10-15% of the hybrids showed higher resistance to the fungal species tested and lower contamination level of their toxins. The lacking correlations between resistance to different fungi and toxins suggest that resistance to different fungi and response to toxin contamination inherits independently, so a toxin analysis is necessary. For safety risk estimation, separated artificial and natural kernel infection and toxin data are needed against all pathogens. Higher resistance to A. flavus and F. verticillioides stabilizes or improves feed safety in hot and dry summers, balancing the harmful effect of climate changes. Resistance and toxin tests during variety registration is an utmost necessity. The exclusion of susceptible or highly susceptible hybrids from commercial production results in reduced toxin contamination.

Reproducibility-optimized detection of differential DNA methylation.

Aim: DNA methylation is a key epigenetic mechanism regulating gene expression. Identifying differentially methylated regions is integral to DNA methylation analysis and there is a need for robust tools reliably detecting regions with significant differences in their methylation status. Materials & methods: We present here a reproducibility-optimized test statistic (ROTS) for detection of differential DNA methylation from high-throughput sequencing or array-based data. Results: Using both simulated and real data, we demonstrate the ability of ROTS to identify differential methylation between sample groups. Conclusion: Compared with state-of-the-art methods, ROTS shows competitive sensitivity and specificity in detecting consistently differentially methylated regions.

An Extract Purified from the Mycelium of a Tomato Wilt-Controlling Strain of Fusarium sambucinum Can Protect Wheat against Fusarium and Common Root Rots.

An approach to manage seed-transmitted Fusarium crown-foot-root rot (FCR, Fusarium spp.) and common root rot (CRR, Bipolaris sorokiniana) on wheat, avoiding environmental risks of chemicals, is seed treatments with microbial metabolites. F. sambucinum strain FS-94 that induces resistance to tomato wilt was shown by this study to be a source of non-fungitoxic wheat-protecting metabolites, which were contained in a mycelium extract purified by gel-chromatography and ultrafiltration. Plant-protecting effect of the purified mycelial extract (PME) was demonstrated in vegetation experiments using a rolled-towel assay and by small-plot field trials. To elucidate mechanisms putatively underlying PME protective activity, tests with cultured Triticum aestivum and T. kiharae cells, particularly the extracellular alkalinization assay, as well as gene expression analysis in germinated wheat seeds were used. Pre-inoculation treatments of seeds with PME significantly decreased the incidence (from 30 to 40%) and severity (from 37 to 50%) of root rots on seedlings without any inhibition of the seed germination and potentiation of deoxynivalenol (DON), DON monoacetylated derivatives and zearalenon production in FCR agents. In vegetation experiments, reductions in the DON production were observed with doses of 0.5 and 1 mg/mL of PME. Pre-sowing PME application on seeds of two spring wheat cultivars naturally infected with FCR and CRR provided the mitigation of both diseases under field conditions during four growing seasons (2013⁻2016). PME-induced ion exchange response in cultured wheat cells, their increased survivability, and up-regulated expression of some defensins' genes in PME-exposed seedlings allow the suggestion of the plant-mediated character of disease-controlling effect observed in field.

Genome-Wide Association Study on Resistance to Stalk Rot Diseases in Grain Sorghum.

Stalk rots are important biotic constraints to sorghum production worldwide. Several pathogens may be associated with the disease, but Macrophomina phaseolina and Fusarium thapsinum are recognized as the major causal organisms. The diseases become more aggressive when drought and high-temperature stress occur during grain filling. Progress in genetic improvement efforts has been slow due to lack of effective phenotyping protocol and the strong environmental effect on disease incidence and severity. Deployment of modern molecular tools is expected to accelerate efforts to develop resistant hybrids. This study was aimed at identifying genomic regions associated with resistance to both causal organisms. A sorghum diversity panel consisting of 300 genotypes assembled from different parts of the world was evaluated for response to infection by both pathogens. Community resources of 79,132 single nucleotide polymorphic (SNP) markers developed on the panel were used in association studies using a multi-locus mixed model to map loci associated with stalk rot resistance. Adequate genetic variation was observed for resistance to both pathogens. Structure analysis grouped the genotypes into five subpopulations primarily based on the racial category of the genotypes. Fourteen loci and a set of candidate genes appear to be involved in connected functions controlling plant defense response. However, each associated SNP had relatively small effect on the traits, accounting for 19-30% of phenotypic variation. Linkage disequilibrium analyses suggest that significant SNPs are genetically independent. Estimation of frequencies of associated alleles revealed that durra and caudatum subpopulations were enriched for resistant alleles, but the results suggest complex molecular mechanisms underlying resistance to both pathogens.

Characterization of Erwinia Populations from Nursery Retention Ponds and Lakes Infecting Ornamental Plants in Florida.

Water shortages in Florida are occurring due to intense utilization of the aquifer system by municipalities and agriculture, and because of continued deficits in annual rainfall. Water districts therefore, are, recommending the use of recycled irrigation water, stormwater runoff, reclaimed municipal sewage water, and lakes for agricultural use. With recycled water, however, there is potential for both introducing and concentrating plant pathogens. In Florida, Erwinia soft-rot bacteria (synonym Pectobacterium) cause extensive crop losses in ornamental plant production. To determine Erwinia spp. population levels, samples were taken monthly for 1 year from four hypereutropic lakes and eight nursery retention ponds. Seventy-seven Erwinia strains were collected by both direct plating and by an enrichment process. With the direct plating method, 0 to 29 CFU/ml were detected on sodium polypectate medium. Significantly higher populations of Erwinia were detected in retention ponds of nurseries that were actively reutilizing their water. Erwinia strains were identified to species by fatty acid analysis and biochemical tests. Strains were further characterized by repetitive element-polymerase chain reaction (rep-PCR) and compared with 120 strains of Erwinia collected from ornamentals over a 17-year period in Florida. Using rep-PCR, most strains were clustered into two heterogeneous populations of E. chrysanthemi and E. carotovora subsp. carotovora in a 1:2 and a 1:4 ratio for isolates from ornamentals and from water, respectively. Within each population of E. chrysanthemi and E. carotovora, genetically different subpopulations could be identified that contained high percentages of Erwinia strains from water sources. Even though genetic differences exist, 99% of the strains from water sources were found to be pathogenic on dieffenbachia. Without water treatment of irrigation and stormwater runoff, there is a potential for both introducing and concentrating Erwinia populations within these water sources.

Adubação nitrogenada sobre a ocorrência de doenças em pós-colheita do morango / Effect of nitrogen doses on the occurrence of postharvest diseases in strawberry

Avaliou-se o efeito de doses de nitrogênio, aplicadas em campo, sobre as doenças pós-colheita nos cultivares de morango Camino Real, Festival e Oso Grande. O nitrogênio, tendo como fonte a ureia, foi avaliado na dose recomendada total (100%), com base na recomendação oficial para a cultura, e mais 3 doses inferiores (83, 67 e 50% do recomendado), tanto no plantio como em cobertura. Os frutos foram colhidos e pesados com 75% da superfície de cor vermelho-brilhante. Doze frutos por parcela foram amostrados em 3 coletas a partir da segunda florada e avaliados quanto à incidência das doenças durante 5 dias de armazenamento a 25°C e 85% de umidade relativa. A identificação das doenças foi feita diariamente com base nos sintomas e sinais dos patógenos. Não foi observado efeito das doses de nitrogênio na produção de frutos e na incidência das doenças, expressa em área abaixo da curva de progresso da doença (AACPD). A maior produção foi observada nos cultivares Festival e Oso Grande. A incidência total de doenças não diferiu significativamente entre os cultivares. O mofo cinzento foi a principal doença, com incidência superior a 30%, seguido da podridão mole de Rhizopus e podridão de levedura.(AU)

This study evaluated the effect of nitrogen doses on postharvest diseases of strawberry cultivars Camino Real, Festival and Oso Grande. The nitrogen in urea formulation was evaluated at the recommended dose (100%) based on the official recommendation to the culture, and 3 lower doses (83, 67 and 50% of recommended), considering the fertilization at planting and cover applications. Fruits were harvested and weighed at 75% of its surface with a bright red color. Twelve fruits per plot were sampled in 3 collections from the second flowering and assessed for disease incidence during five days of storage at 25°C and 85% relative humidity. The identification of disease was made daily, based on symptoms and pathogen signs. There was no effect of nitrogen doses on yield and incidence of postharvest diseases, expressed as area under the disease progress curve (AUDPC). Increased average production was observed in cultivars Festival and Oso Grand. The overall incidence of disease among the cultivars did not differ significantly. Gray mold was the main disease, affecting more than 30% of fruits, followed by Rhizopus and yeast rot.(AU)