RESUMO
A 78-year-old woman with hypertrophic cardiomyopathy underwent a septal myomectomy and valve replacement. In the immediate postoperative period she developed shock of mixed etiology and died. At autopsy, hepatomegaly and splenomegaly were identified, with PAS and Giemsa positive intracellular ceroid granular deposits. Sea-blue histiocytosis is an extremely rare, chronic and benign deposit disease. It is characterized by hepatosplenomegaly, thrombocytopenia and lymphadenopathy. The presence of ceroid substance in granules in PAS and Giemsa stains should establish the diagnosis of suspicion.
Assuntos
Síndrome do Histiócito Azul-Marinho , Feminino , Humanos , Idoso , Síndrome do Histiócito Azul-Marinho/complicações , Síndrome do Histiócito Azul-Marinho/diagnóstico , Ceroide , Esplenomegalia/complicações , Hepatomegalia/etiologiaRESUMO
[Figure: see text].
Assuntos
Artérias Carótidas/metabolismo , Estenose das Carótidas/metabolismo , Ceroide/metabolismo , Peroxidação de Lipídeos , Lipoproteínas LDL/metabolismo , Macrófagos/metabolismo , Microscopia de Fluorescência , Imagem Óptica , Placa Aterosclerótica , Espectroscopia de Luz Próxima ao Infravermelho , Idoso , Artérias Carótidas/patologia , Estenose das Carótidas/patologia , Feminino , Humanos , Macrófagos/patologia , Masculino , Pessoa de Meia-Idade , Células THP-1RESUMO
Atherosclerotic plaque formation starts early in life, develops silently over decades, and often displays clear evidence of accelerated biological aging. Lipofuscin has been classically defined as "the most consistent and phylogenetically conserved cellular morphologic change of aging," however, despite this traditional view different lines of evidence have recently demonstrated that, besides aging, various noxious influences can engeder its accumulation in cells and also that specific experimental conditions can revert this effect. Lipofuscin has been also proven to interact with disease-related factors to enhance cell loss. Along with lipofuscin, ceroid, another autofluorescent lipopigment usually produced under various pathological conditions unrelated to aging, has been suggested to jeopardize cell performance and viability by inducing membrane fragility, mitochondrial dysfunction, DNA damage, and oxidative stress-induced apoptosis. With regard to atherosclerosis, very few investigations have been conducted to assess whether a link could exist between lipofuscin/ceroid accumulation and the progression of the disease and no information still exist regarding the anatomy and the ultrastructural diversification of lipofuscin and ceroid in the lesional vascular tissue. At the same time, data concerning their potential toxicity at the cellular level are fragmentary, dated, and scarce. The present study investigates the occurrence and distribution of lipofuscin and ceroid in human atherosclerotic plaque and adjacent healthy tissues and analyzes the ultrastructural changes associated with their accumulation within the cell.
Assuntos
Aterosclerose/patologia , Ceroide/metabolismo , Lipofuscina/metabolismo , Placa Aterosclerótica/patologia , Adulto , Idoso , Envelhecimento/fisiologia , Aterosclerose/metabolismo , Feminino , Fibroblastos/metabolismo , Fibroblastos/ultraestrutura , Humanos , Lisossomos/metabolismo , Lisossomos/ultraestrutura , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica/metabolismoRESUMO
Autofluorescent storage material (ASM) is an aging pigment that accumulates during the normal course of senescence. Although the role of ASM has yet to be fully elucidated, ASM has been implicated in age-related neurodegeneration. In this study, we determined the level of ASM in chloride channel 3 (ClC-3) gene-deficient (KO) mice both in response to aging and following mild global ischemia. To understand the mechanism of action of the ASM, mice subjected to ischemia were treated with the cyclooxygenase (COX) inhibitor indomethacin or with the noncompetitive glutamate receptor antagonist MK-801. ClC-3 KO mice displayed age-related neurodegeneration of the neocortex as well as the hippocampus. The cortical layers in particular granular layers became thinner with aging. ASM accumulated in the brains of ClC-3 KO mice was increased seven- to 50-fold over that observed in the corresponding regions of their wild-type littermates. Young wild-type mice survived longer than age-matched ClC-3 KO mice after permanent global ischemia. However, in the case of older animals, the survival curves were similar. The ASM also increased four- to fivefold 10 days after mild global ischemia, an effect that was suppressed by treatment with indomethacin and MK-801. These results suggest that temporary ischemia might trigger a process similar to aging in the brain, mimicking the effect of age-related neurodegenerative diseases.
Assuntos
Envelhecimento/genética , Isquemia Encefálica/genética , Encéfalo/patologia , Ceroide/análise , Canais de Cloreto/deficiência , Lipofuscina/análise , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Encéfalo/metabolismo , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Canais de Cloreto/biossíntese , Canais de Cloreto/genética , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos ICR , Camundongos Knockout , Degeneração Neural/genética , Degeneração Neural/metabolismo , Degeneração Neural/patologia , Imagem ÓpticaRESUMO
Cellular deposits of oxidized and aggregated proteins are hallmarks of a variety of age-related disorders, but whether such proteins contribute to pathology is not well understood. We previously reported that oxidized proteins form lipofuscin/ceroid-like bodies with a lysosomal-type distribution and up-regulate the transcription and translation of proteolytic lysosomal enzymes in cultured J774 mouse macrophages. Given the recently identified role of lysosomes in the induction of apoptosis, we have extended our studies to explore a role for oxidized proteins in apoptosis. Oxidized proteins were biosynthetically generated in situ by substituting oxidized analogues for parent amino acids. Apoptosis was measured with Annexin-V/PI (propidium iodide), TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling), MMP (mitochondrial membrane permeabilization), caspase activation and cytochrome c release, and related to lysosomal membrane permeabilization. Synthesized proteins containing the tyrosine oxidation product L-DOPA (L-3,4-dihydroxyphenylalanine) were more potent inducers of apoptosis than proteins containing the phenylalanine oxidation product o-tyrosine. Apoptosis was dependent upon incorporation of oxidized residues, as indicated by complete abrogation in cultures incubated with the non-incorporation control D-DOPA (D-3,4-dihydroxyphenylalanine) or when incorporation was competed out by parent amino acids. The findings of the present study suggest that certain oxidized proteins could play an active role in the progression of age-related disorders by contributing to LMP (lysosomal membrane permeabilization)-initiated apoptosis and may have important implications for the long-term use of L-DOPA as a therapeutic agent in Parkinson's disease.
Assuntos
Apoptose , Levodopa/efeitos adversos , Levodopa/metabolismo , Monócitos/metabolismo , Biossíntese de Proteínas , Tirosina/efeitos adversos , Tirosina/metabolismo , Envelhecimento , Anexina A5/metabolismo , Caspase 3/metabolismo , Linhagem Celular , Ceroide/efeitos adversos , Fragmentação do DNA , Ativação Enzimática , Humanos , Membranas Intracelulares , Lipofuscina/efeitos adversos , Lisossomos , Potencial da Membrana Mitocondrial , Membranas Mitocondriais , Oxirredução , PermeabilidadeRESUMO
Deficiency in Cathepsin D (CtsD), the major cellular lysosomal aspartic proteinase, causes the congenital form of neuronal ceroid lipofuscinoses (NCLs). CtsD-deficient mice show severe visceral lesions like lymphopenia in addition to their central nervous system (CNS) phenotype of ceroid accumulation, microglia activation, and seizures. Here we demonstrate that re-expression of CtsD within the CNS but not re-expression of CtsD in visceral organs prevented both central and visceral pathologies of CtsD(-/-) mice. Our results suggest that CtsD was substantially secreted from CNS neurons and drained from CNS to periphery via lymphatic routes. Through this drainage, CNS-expressed CtsD acts as an important modulator of immune system maintenance and peripheral tissue homeostasis. These effects depended on enzymatic activity and not on proposed functions of CtsD as an extracellular ligand. Our results furthermore demonstrate that the prominent accumulation of ceroid/lipofuscin and activation of microglia in brains of CtsD(-/-) are not lethal factors but can be tolerated by the rodent CNS.
Assuntos
Catepsina D/metabolismo , Sistema Nervoso Central/metabolismo , Modelos Animais de Doenças , Linfopenia/metabolismo , Lipofuscinoses Ceroides Neuronais/metabolismo , Animais , Catepsina D/genética , Sistema Nervoso Central/patologia , Ceroide/metabolismo , Dependovirus/genética , Dependovirus/metabolismo , Vetores Genéticos , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microglia/metabolismo , Lipofuscinoses Ceroides Neuronais/patologia , Neurônios/metabolismo , Taxa de Sobrevida , Timo/citologia , Distribuição Tecidual , Vísceras/metabolismo , Vísceras/patologiaRESUMO
Neuronal ceroid lipofuscinoses (NCLs) are the most common hereditary neurodegenerative diseases of childhood. The infantile form, INCL, is caused by lysosomal palmitoyl-protein thioesterase (PPT) deficiency, which impairs the cleavage of thioester linkages in palmitoylated proteins, preventing their hydrolysis by lysosomal proteinases. Consequent accumulation of these lipid-modified proteins (constituents of ceroid) in lysosomes leads to INCL. Because thioester linkages are susceptible to nucleophilic attack, drugs with this property may have therapeutic potential for INCL. We report here that two such drugs, phosphocysteamine and N-acetylcysteine, disrupt thioester linkages in a model thioester compound, [14C]palmitoyl approximately CoA. Most importantly, in lymphoblasts derived from INCL patients, phosphocysteamine, a known lysosomotrophic drug, mediates the depletion of lysosomal ceroids, prevents their re-accumulation and inhibits apoptosis. Our results define a novel pharmacological approach to lysosomal ceroid depletion and raise the possibility that nucleophilic drugs such as phosphocysteamine hold therapeutic potential for INCL.
Assuntos
Ceroide/metabolismo , Lipofuscinoses Ceroides Neuronais/tratamento farmacológico , Lipofuscinoses Ceroides Neuronais/metabolismo , Acetilcisteína/farmacologia , Apoptose/efeitos dos fármacos , Células Cultivadas , Criança , Códon sem Sentido , Cistafos/farmacologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Fibroblastos/patologia , Glicoproteínas/metabolismo , Humanos , Lactente , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Linfócitos/patologia , Lisossomos/efeitos dos fármacos , Lisossomos/metabolismo , Mutação de Sentido Incorreto , Lipofuscinoses Ceroides Neuronais/patologia , Palmitoil Coenzima A/metabolismo , Palmitoil-CoA Hidrolase/deficiência , Palmitoil-CoA Hidrolase/genética , SaposinasRESUMO
Chediak-Higashi syndrome is characterized by oculocutaneous albinism, a bleeding tendency and severe recurrent infections. Age-dependent formations of autofluorescent ceroid-like substances have been noted in a variety of tissues. In this study, we isolated an autofluorescent ceroid-like aggregate from purified Beige mouse liver lysosomes and analyzed the composition of the aggregate by ion trap mass-spectrometry. In addition to lysosomal proteins, this aggregate contains proteins normally localized in the ER, mitochondria, peroxisomes, and the cytosol. Bip, a luminal ER protein was abundant in lysosomal ceroid. The ER, mitochondria, and cytosol proteins could arise in lysosomes through stimulation of autophagy, but we found no differences between normal and CHS fibroblasts in the degree of lysosomal acidity and in the level of conversion of soluble microtubular-associated protein 1 light chain 3 type I to membrane-associated type II, an accepted probe for hyper-autophagy suggesting that ceroid formation is unlikely to arise via this mechanism.
Assuntos
Ceroide/metabolismo , Síndrome de Chediak-Higashi/metabolismo , Fígado/metabolismo , Lisossomos/metabolismo , Animais , Autofagia , Células Cultivadas , Modelos Animais de Doenças , Fluorescência , Espectrometria de Massas , Camundongos , Proteínas/metabolismo , ProteômicaRESUMO
An 8-year-old neutered female domestic shorthair cat was presented for further management of an apocrine gland cystadenocarcinoma. Extensive nodal metastasis was diagnosed and the cat was humanely destroyed 2 months after presentation. Post-mortem histopathology of the cystadenocarcinoma revealed areas of yellow-brown granular pigmentation on light microscopy, staining positively for reducing substances with Schmorl's stain and demonstrating autofluorescence on confocal microscopy. The cat's urine was black and also exhibited autofluorescence, and further analysis revealed increased free pentosidine. Based on these findings, it was presumed that the apocrine gland cystadenocarcinoma was producing lipofuscin-like pigments and that the characteristics of the urine were at least partially secondary to advanced glycation end-products.
Assuntos
Glândulas Apócrinas/patologia , Doenças do Gato/patologia , Cistadenocarcinoma/veterinária , Neoplasias das Glândulas Sudoríparas/veterinária , Animais , Gatos , Ceroide/urina , Feminino , Produtos Finais de Glicação Avançada/urina , PigmentaçãoRESUMO
Botswana's Okavango Delta is a World Heritage Site and biodiverse wilderness. In 2016-2018, following arrival of the annual flood of rainwater from Angola's highlands, and using continuous oxygen logging, we documented profound aquatic hypoxia that persisted for 3.5 to 5 months in the river channel. Within these periods, dissolved oxygen rarely exceeded 3 mg/L and dropped below 0.5 mg/L for up to two weeks at a time. Although these dissolved oxygen levels are low enough to qualify parts of the Delta as a dead zone, the region is a biodiversity hotspot, raising the question of how fish survive. In association with the hypoxia, histological samples, collected from native Oreochromis andersonii (threespot tilapia), Coptodon rendalli (redbreast tilapia), and Oreochromis macrochir (greenhead tilapia), exhibited widespread hepatic and splenic inflammation with marked granulocyte infiltration, melanomacrophage aggregates, and ceroid and hemosiderin accumulations. It is likely that direct tissue hypoxia and polycythemia-related iron deposition caused this pathology. We propose that Okavango cichlids respond to extended natural hypoxia by increasing erythrocyte production, but with significant health costs. Our findings highlight seasonal hypoxia as an important recurring stressor, which may limit fishery resilience in the Okavango as concurrent human impacts rise. Moreover, they illustrate how fish might respond to hypoxia elsewhere in the world, where dead zones are becoming more common.
Assuntos
Oxigênio/química , Tilápia/metabolismo , Animais , Ceroide/metabolismo , Eritrócitos/citologia , Eritrócitos/metabolismo , Feminino , Hemossiderina/metabolismo , Hipóxia , Ferro/metabolismo , Fígado/metabolismo , Fígado/patologia , Masculino , Oxigênio/metabolismo , Rios , Baço/metabolismo , Baço/patologiaRESUMO
Acetyl ethyl tetramethyl tetralin (AETT), a component of soaps, deodorants, and cosmetics, produces hyperirritability and limb weakness in rats repeatedly exposed to the compound. Brain, spinal cord, and peripheral nerves are discolored blue, show progressive neuronal ceroid degeneration, and develop spectacular myelin bubbling. These neurotoxic properties of AETT provide the basis for industry's decision to withdraw the compound from consumer products. In addition, AETT offers the experimentalist a new probe to explore the etiology and pathogeneses of human ceroid and myelin diseases.
Assuntos
Doenças Desmielinizantes/induzido quimicamente , Naftalenos/toxicidade , Perfumes/toxicidade , Transtornos da Pigmentação/induzido quimicamente , Tetra-Hidronaftalenos/toxicidade , Animais , Ceroide , Doenças Desmielinizantes/patologia , Transtornos dos Movimentos/induzido quimicamente , Neurotoxinas , Ratos , Células de Schwann/patologiaRESUMO
The most striking morphologic change in neurons during normal aging is the accumulation of autophagic vacuoles filled with lipofuscin or neuromelanin pigments. These organelles are similar to those containing the ceroid pigments associated with neurologic disorders, particularly in diseases caused by lysosomal dysfunction. The pigments arise from incompletely degraded proteins and lipids principally derived from the breakdown of mitochondria or products of oxidized catecholamines. Pigmented autophagic vacuoles may eventually occupy a major portion of the neuronal cell body volume because of resistance of the pigments to lysosomal degradation and/or inadequate fusion of the vacuoles with lysosomes. Although the formation of autophagic vacuoles via macroautophagy protects the neuron from cellular stress, accumulation of pigmented autophagic vacuoles may eventually interfere with normal degradative pathways and endocytic/secretory tasks such as appropriate response to growth factors.
Assuntos
Envelhecimento/metabolismo , Autofagia/fisiologia , Encefalopatias/metabolismo , Encéfalo/metabolismo , Neurônios/metabolismo , Vacúolos/metabolismo , Animais , Encéfalo/patologia , Encéfalo/ultraestrutura , Encefalopatias/patologia , Ceroide/metabolismo , Corantes/metabolismo , Humanos , Lipofuscina/metabolismo , Melaninas/metabolismo , Neurônios/patologia , Neurônios/ultraestrutura , Vacúolos/patologia , Vacúolos/ultraestruturaRESUMO
PURPOSE: Late infantile neuronal ceroid lipofuscinosis (NCL) is an inherited disorder characterized by progressive vision loss. The disease results from mutations in the TPP1 (CLN2) gene. Studies were undertaken to characterize the effects of a TPP1 frameshift mutation on the retina in Dachshunds. METHODS: A litter of four puppies consisting of one homozygous affected dog, two heterozygotes, and one homozygous normal dog were monitored for neurologic and retinal changes through 10 months of age. The affected and homozygous normal dogs, as well as one of the heterozygotes, were then euthanatized, and the retinas were examined morphologically. RESULTS: The affected dog exhibited normal visual behavior and retinal function at 3 months of age, but vision was clearly impaired by 7 months, with markedly reduced ERG b-wave amplitudes. Beyond 7 months of age, the affected dog was functionally blind, and pupillary light reflexes and ERG response amplitudes continued to decline through 10 months of age. Both rod and cone system functions were severely impaired. The retina exhibited accumulation of autofluorescent storage bodies with distinctive curvilinear contents. Substantial cell loss occurred in the inner nuclear layer, with a smaller reduction in photoreceptor cell density. CONCLUSIONS: The canine TPP1 mutation results in progressive vision loss and retinal degeneration similar to that which occurs in human late infantile NCL. With the canine model, the natural history of disease progression in the retina provides a better understanding of the pathologic course of the disease and provides objective markers that can be used to assess the efficacy of therapeutic interventions.
Assuntos
Doenças do Cão/patologia , Lipofuscinoses Ceroides Neuronais/veterinária , Doenças Retinianas/veterinária , Aminopeptidases , Animais , Ceroide/metabolismo , Dipeptidil Peptidases e Tripeptidil Peptidases , Modelos Animais de Doenças , Doenças do Cão/genética , Cães , Eletrorretinografia/veterinária , Endopeptidases/genética , Endopeptidases/metabolismo , Feminino , Mutação da Fase de Leitura , Lipofuscina/metabolismo , Masculino , Lipofuscinoses Ceroides Neuronais/genética , Lipofuscinoses Ceroides Neuronais/patologia , Retina/metabolismo , Retina/patologia , Doenças Retinianas/genética , Doenças Retinianas/patologia , Serina Proteases , Tripeptidil-Peptidase 1RESUMO
The artificial ceroid/lipofuscin pigments originated from different organ tissues, including liver, brain, heart, and kidney of rats, and biomaterials were studied with improved fluorometric techniques. With all tissue materials exposed under ultraviolet (UV) light, a series of similar fluorescent colors were observed under microfluorometer. Analogous fluorescence spectra were also demonstrated with a three-dimensional (3-D) front-surface fluorometric technique despite of the tissue differences. Measured with 3-D fluorometry, relatively simple lipofuscin-like fluorophores were observed from the reactions of malondialdehyde (MDA) with critical biological macromolecules, such as bovine serum albumin (BSA) and DNA. Our results demonstrated that the biomaterials from different tissues have a similar fate under accelerated oxidative/carbonyl stresses but may be differentiated by a fluorescence intensity ratio.
Assuntos
Envelhecimento/metabolismo , Ceroide/análise , Lipofuscina/análise , Animais , Ceroide/biossíntese , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos da radiação , Lipofuscina/biossíntese , Masculino , Malondialdeído/farmacologia , Pigmentos Biológicos/análise , Pigmentos Biológicos/biossíntese , Ratos , Ratos Sprague-Dawley , Projetos de Pesquisa , Espectrometria de Fluorescência/métodos , Raios UltravioletaRESUMO
Oxidised low density lipoprotein (LDL) was considered to be important in the pathogenesis of atherosclerosis, but the large clinical trials of antioxidants, including the first one using probucol (the PQRST Trial), failed to show benefit and have cast doubt on the importance of oxidised LDL. We have shown previously that LDL oxidation can be catalysed by iron in the lysosomes of macrophages. The aim of this study was therefore to investigate the effectiveness of antioxidants in preventing LDL oxidation at lysosomal pH and also establish the possible mechanism of oxidation. Probucol did not effectively inhibit the oxidation of LDL at lysosomal pH, as measured by conjugated dienes or oxidised cholesteryl esters or tryptophan residues in isolated LDL or by ceroid formation in the lysosomes of macrophage-like cells, in marked contrast to its highly effective inhibition of LDL oxidation at pH 7.4. LDL oxidation at lysosomal pH was inhibited very effectively for long periods by N,N'-diphenyl-1,4-phenylenediamine, which is more hydrophobic than probucol and has been shown by others to inhibit atherosclerosis in rabbits, and by cysteamine, which is a hydrophilic antioxidant that accumulates in lysosomes. Iron-induced LDL oxidation might be due to the formation of the superoxide radical, which protonates at lysosomal pH to form the much more reactive, hydrophobic hydroperoxyl radical, which can enter LDL and reach its core. Probucol resides mainly in the surface monolayer of LDL and would not effectively scavenge hydroperoxyl radicals in the core of LDL. This might explain why probucol failed to protect against atherosclerosis in various clinical trials. The oxidised LDL hypothesis of atherosclerosis now needs to be re-evaluated using different and more effective antioxidants that protect against the lysosomal oxidation of LDL.
Assuntos
Antioxidantes/química , Lipoproteínas LDL/química , Lisossomos/química , Animais , Antioxidantes/uso terapêutico , Aterosclerose/tratamento farmacológico , Linhagem Celular , Ceroide/química , Cromatografia Líquida de Alta Pressão , Cisteamina/química , Compostos Ferrosos/química , Humanos , Peróxido de Hidrogênio/química , Concentração de Íons de Hidrogênio , Lipoproteínas LDL/análise , Oxirredução , Probucol/química , Probucol/uso terapêutico , CoelhosRESUMO
Accumulation of intracellular autofluorescent material or "aging pigment" has been characterized as a normal aging event. Certain diseases also exhibit a similar accumulation of intracellular autofluorescent material. However, autofluorescent storage material associated with aging and disease has distinct characteristics. Lipofuscin is a common term for aging pigments, whereas ceroid is used to describe pathologically derived storage material, for example, in the neuronal ceroid lipofuscinoses (NCLs). NCLs are a family of neurodegenerative diseases that are characterized by an accumulation of autofluorescent storage material (ceroid) in the lysosome, which has been termed "lipofuscin-like". There have been many studies that describe this autofluorescent storage material, but what is it? Is this accumulation lipofuscin or ceroid? In this review we will try to answer the following questions: (1) What is lipofuscin and ceroid? (2) What contributes to the accumulation of this storage material in one or the other? (3) Does this material have an effect on cellular function? Studying parallels between the accumulation of lipofuscin and ceroid may provide insight into the biological relevance of these phenomena.
Assuntos
Ceroide/análise , Lipofuscina/análise , Lisossomos/metabolismo , Lipofuscinoses Ceroides Neuronais/metabolismo , Envelhecimento/fisiologia , Animais , Autofagia/fisiologia , Humanos , Modelos Biológicos , Doenças do Sistema Nervoso/metabolismo , Lipofuscinoses Ceroides Neuronais/genética , OxirreduçãoRESUMO
Fluorescent characteristics of age pigment-related materials were re-examined with improved techniques. A series of fluorescent colors, from blue to yellow-red were observed in artificial ceroid/lipofuscin. A front-surface accessory attached to a spectrofluorometer was found very useful in studying the age pigment-like aggregates directly in its solid state. With a three-dimensional (3D)-fluorescence measurement, in addition to the front-surface application, entire fluorescence spectra of artificial ceroid/lipofuscin both in extractions and in non-extractable tissues were obtained. When the front-surface 3D-scan technique was applied to estimate collagen-related age pigments of rat-tails in situ, a dynamic process of age-related protein deterioration accompanied with age pigment development was recorded. The front-surface 3D-fluorescence technique introduced in this study may be used as a practical and effective tool in studying in situ pigment alterations during aging process.
Assuntos
Envelhecimento/metabolismo , Pigmentos Biológicos/análise , Animais , Ceroide/análise , Colágeno/análise , Cor , Lipofuscina/análise , Ratos , Ratos Sprague-Dawley , Espectrometria de Fluorescência/métodos , Cauda , Raios UltravioletaRESUMO
Cerebral lipopigments (LPs)--lipofuscin and ceroid--represent a significant marker in postmitotic normal and pathologic aging, connected with causal and associated neuropathologic damage. Therefore, LP processing, lysis, and elimination may be the main targets in anti-aging and rejuvenation therapies. The regenerative neuroactive factors improve neuron supply with specific nutrients from plasma. They enhance the antioxidative defense, have anti-LP-poietic actions, stimulate brain anabolism, support energetic metabolism, and elevate the reduced lysosomal enzymes. In the second stage, by cytoplasm rehydration, they initiate the breaking up of the neuronal aggregated LP conglomerates, by consecutive disintegration. Then, possibly by the localized exo-endocytosis process between neurons and adjacent glia (especially microglia), intercellular LP transfer can be realized. So, therapeutically activated glia turn into brain garbage collectors and transporters. Therapeutic processing of glial LPs increases in the capillary neighborhood. Highly processed LPs, by glio-endothelial transfer, reach capillary walls before being eliminated. Consequently, neuroactive therapies having these synergistic rejuvenative actions represent new prospects in deceleration of normal and pathological cerebral aging.
Assuntos
Envelhecimento/fisiologia , Encéfalo/metabolismo , Lipídeos/análise , Rejuvenescimento/fisiologia , Animais , Ceroide/metabolismo , Lipofuscina/metabolismoRESUMO
Neuronal ceroid lipopofuscinosis (Batten disease, NCL) represents a group of common childhood neurodegenerative diseases with a shared feature of deposition of abnormal metabolic products in neurons and other tissues, including peripheral blood lymphocytes. In most forms of NCL no specific enzyme defect is known and the diagnosis relies primarily on ultrastructural identification of characteristic membrane-bound inclusions containing the abnormal metabolic product. All buffy-coat specimens examined during a 7-year period (1997-2004) for the exclusion or confirmation of the diagnosis NCL were reviewed. From a total of 265 samples, 9 were inadequate and NCL was diagnosed in 56. Five showed granular osmophilic deposits of infantile Batten disease (NCL1), 10 showed curvilinear profiles of classical late infantile Batten disease (NCL2), and 17 showed vacuolated lymphocytes with fingerprint profiles, indicating classical juvenile Batten disease (NCL3). 24 samples (43%) demonstrated compact electron-dense deposits with fingerprint profiles in the absence of vacuolated lymphocytes, indicative of variant forms NCL. Ultrastructual examination of peripheral blood allows reliable and specific diagnosis of subtypes of Batten disease, including variants, and is a useful, minimally invasive test for the diagnosis of NCL in childhood.
Assuntos
Ceroide/metabolismo , Corpos de Inclusão/ultraestrutura , Leucócitos Mononucleares/ultraestrutura , Microscopia Eletrônica de Transmissão , Lipofuscinoses Ceroides Neuronais/diagnóstico , Pré-Escolar , Humanos , Corpos de Inclusão/metabolismo , Lactente , Leucócitos Mononucleares/metabolismo , Lipofuscinoses Ceroides Neuronais/sangueRESUMO
When mouse peritoneal macrophages as well as P388D1 cells, an established macrophage-like cell line, were cultured with liposomes composed of rat liver phosphatidylcholine and phosphatidylserine, storage of fluorescent products, ceroid-like pigments, within those cells was observed with light and fluorescence microscopy, and fluorescence spectrophotometry. The amounts of thiobarbituric acid-reactive substances and fluorescent products in macrophages were increased gradually to reach a maximal level to between 6 and 8 days of culture. The involvement of peroxidation of liposomal lipids in the formation of the pigments was further suggested by the 6 days that incorporation of alpha-tocopherol into liposomes decreased the storage of the pigments. No appreciable formation of the pigments was observed in macrophages cultured with liposomes containing dipalmitoylphosphatidylcholine instead of rat liver phosphatidylcholine. The fluorescent products formed in cultured cells were found in lipid-soluble and -insoluble fractions. Lipid-insoluble fluorescent products had an excitation maximum at 360 nm and a fluorescence maximum at 430 nm in SDS-aqueous solution (pH 7.4) and the intensity of the fluorescence was quenched at base pH, but it was not changed in acidic media. These findings indicate that the macrophages can store Schiff base fluorescent substances formed by the reaction between peroxidation products of exogenous lipids and amino compounds in the cells, under some pathological conditions.