RESUMO
We developed a hue-based pH determination method to analyze digital images of samples in a 384-well plate after the addition of a universal pH indicator. The standard error of calibration for 69 pH standards was 0.078 pH units, and no sample gave an error greater than 0.23 units. We then used in-solution isoelectric focusing to determine the isoelectric point of Wnt3A protein in conditioned medium and after purification and applied the described method to assess the pH of these small-volume samples. End users may access our standard to assay the pH of their own samples with no additional calibration.
Assuntos
Focalização Isoelétrica/métodos , Animais , Calibragem , Bovinos , Concentração de Íons de Hidrogênio , Limite de DetecçãoRESUMO
A combined thread-paper microfluidic device (µTPAD) is presented for the determination of glucose in blood. The device is designed to include all the analytical operations needed: red blood cell separation, conditioning, enzymatic recognition, and colorimetric transduction. The signal is captured with a smartphone or tablet working in video mode and processed by custom Android-based software in real-time. The automatic detection of the region of interest on the thread allows for the use of either initial rate or equilibrium signal as analytical parameters. The time needed for analysis is 12â¯s using initial rate, and 100â¯s using the equilibrium measurement with a LOD of 48⯵M and 12⯵M, respectively, and a precision around 7%. The µTPAD allows a rapid determination of glucose in real samples using only 3⯵L of whole blood.
Assuntos
Glicemia/análise , Dispositivos Lab-On-A-Chip , Smartphone , Colorimetria/instrumentação , Humanos , SoftwareRESUMO
A new spectrometer, here denoted the SLIM (simple, low-power, inexpensive, microcontroller-based) spectrometer, was developed that exploits the small size and low cost of solid-state electronic devices. In this device, light-emitting diodes (LED), single-chip integrated circuit photodetectors, embedded microcontrollers, and batteries replace traditional optoelectronic components, computers, and power supplies. This approach results in complete customizable spectrometers that are considerably less expensive and smaller than traditional instrumentation. The performance of the SLIM spectrometer, configured with a flow cell, was evaluated and compared to that of a commercial spectrophotometer. Thionine was the analyte, and the detection limit was approximately 0.2 microM with a 1.5-mm-path length flow cell. Nonlinearity due to the broad emission profile of the LED light sources is discussed.