RESUMO
Merocyanine dye based fluorescent organic compound has been synthesized for the detection of glutamine. The probe showed remarkable fluorescent intensity with glutamine through ICT (Intermolecular Charge Transfer Mechanism). Hence, it is tested for the detection of glutamine using colorimetric and fluorimetric techniques in physiological and neutral pH (7.2). Under optimized experimental conditions, the probe detects glutamine selectively among other interfering biomolecules. The probe has showed a LOD (lower limit of detection) of 9.6 × 10-8 mol/L at the linear range 0-180 µM towards glutamine. The practical application of the probe is successfully tested in human biofluids.
Assuntos
Técnicas Biossensoriais , Corantes Fluorescentes , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química , Glutamina , Humanos , Limite de Detecção , PirimidinonasRESUMO
A newly designed fluorescent and colorimetric probe was synthesized and selective detection of cu2+ was successful in aqueous medium. The design strategy exhibited strongly fluorescent when binding with cu2+ based on the change in structure between spirocyclic to a non-cyclic form of rhodamine based dye. The UV visible spectra of probe (6GS2) exhibited three absorption peaks at 229, 309 and 530 nm respectively. The emission spectra of fluorescent probe exhibited wavelength at 550 nm. The peak intensity increases during the addition of copper ion to probe through n-π transition. The probe characterized by different techniques like NMR, absorption, emission, mass and test strips methods. Graphical Abstract .