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1.
Mol Cell ; 83(18): 3268-3282.e7, 2023 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-37689068

RESUMO

Heritable non-genetic information can regulate a variety of complex phenotypes. However, what specific non-genetic cues are transmitted from parents to their descendants are poorly understood. Here, we perform metabolic methyl-labeling experiments to track the heritable transmission of methylation from ancestors to their descendants in the nematode Caenorhabditis elegans (C. elegans). We find heritable methylation in DNA, RNA, proteins, and lipids. We find that parental starvation elicits reduced fertility, increased heat stress resistance, and extended longevity in fed, naïve progeny. This intergenerational hormesis is accompanied by a heritable increase in N6'-dimethyl adenosine (m6,2A) on the 18S ribosomal RNA at adenosines 1735 and 1736. We identified DIMT-1/DIMT1 as the m6,2A and BUD-23/BUD23 as the m7G methyltransferases in C. elegans that are both required for intergenerational hormesis, while other rRNA methyltransferases are dispensable. This study labels and tracks heritable non-genetic material across generations and demonstrates the importance of rRNA methylation for regulating epigenetic inheritance.


Assuntos
Caenorhabditis elegans , Hormese , Animais , RNA Ribossômico 18S , Caenorhabditis elegans/genética , Metiltransferases/genética , Adenosina
2.
Genes Dev ; 30(21): 2404-2416, 2016 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-27881602

RESUMO

N-terminal acetylation of the first two amino acids on proteins is a prevalent cotranslational modification. Despite its abundance, the biological processes associated with this modification are not well understood. Here, we mapped the pattern of protein N-terminal acetylation in Caenorhabditis elegans, uncovering a conserved set of rules for this protein modification and identifying substrates for the N-terminal acetyltransferase B (NatB) complex. We observed an enrichment for global protein N-terminal acetylation and also specifically for NatB substrates in the nucleus, supporting the importance of this modification for regulating biological functions within this cellular compartment. Peptide profiling analysis provides evidence of cross-talk between N-terminal acetylation and internal modifications in a NAT substrate-specific manner. In vivo studies indicate that N-terminal acetylation is critical for meiosis, as it regulates the assembly of the synaptonemal complex (SC), a proteinaceous structure ubiquitously present during meiosis from yeast to humans. Specifically, N-terminal acetylation of NatB substrate SYP-1, an SC structural component, is critical for SC assembly. These findings provide novel insights into the biological functions of N-terminal acetylation and its essential role during meiosis.


Assuntos
Caenorhabditis elegans/metabolismo , Acetiltransferase N-Terminal B/metabolismo , Complexo Sinaptonêmico/metabolismo , Acetilação , Animais , Caenorhabditis elegans/enzimologia , Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Núcleo Celular/metabolismo , Meiose/genética , Mutação , Acetiltransferase N-Terminal B/genética , Proteínas Nucleares/metabolismo , Proteoma , Complexo Sinaptonêmico/química , Complexo Sinaptonêmico/genética
3.
Nucleic Acids Res ; 49(2): e9, 2021 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-33264395

RESUMO

There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to monitor and manipulate processes within organs and individual cells. It this study, we developed a labeling-free method for measuring organ- and cell-type-specific translation elongation rates in vivo. It is based on time-resolved delivery of translation initiation and elongation inhibitors in live animals followed by ribosome profiling. It also reports translation initiation sites in an organ-specific manner. Using this method, we found that the elongation rates differ more than 50% among mouse organs and determined them to be 6.8, 5.0 and 4.3 amino acids per second for liver, kidney, and skeletal muscle, respectively. We further found that the elongation rate is reduced by 20% between young adulthood and mid-life. Thus, translation, a major metabolic process in cells, is tightly regulated at the level of elongation of nascent polypeptide chains.


Assuntos
Envelhecimento/metabolismo , Rim/metabolismo , Fígado/metabolismo , Músculo Esquelético/metabolismo , Elongação Traducional da Cadeia Peptídica , Envelhecimento/genética , Animais , Análise por Conglomerados , Cavidades Cranianas , Cicloeximida/administração & dosagem , Cicloeximida/farmacologia , Esquema de Medicação , Harringtoninas/administração & dosagem , Harringtoninas/farmacologia , Sequenciamento de Nucleotídeos em Larga Escala , Injeções Intravenosas , Cinética , Longevidade , Macrolídeos/administração & dosagem , Macrolídeos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Órbita , Especificidade de Órgãos , Elongação Traducional da Cadeia Peptídica/efeitos dos fármacos , Iniciação Traducional da Cadeia Peptídica , Piperidonas/administração & dosagem , Piperidonas/farmacologia , Ribossomos/metabolismo , Cauda , Transcriptoma
4.
Proc Natl Acad Sci U S A ; 117(27): 15581-15590, 2020 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-32576685

RESUMO

Protein synthesis represents a major metabolic activity of the cell. However, how it is affected by aging and how this in turn impacts cell function remains largely unexplored. To address this question, herein we characterized age-related changes in both the transcriptome and translatome of mouse tissues over the entire life span. We showed that the transcriptome changes govern those in the translatome and are associated with altered expression of genes involved in inflammation, extracellular matrix, and lipid metabolism. We also identified genes that may serve as candidate biomarkers of aging. At the translational level, we uncovered sustained down-regulation of a set of 5'-terminal oligopyrimidine (5'-TOP) transcripts encoding protein synthesis and ribosome biogenesis machinery and regulated by the mTOR pathway. For many of them, ribosome occupancy dropped twofold or even more. Moreover, with age, ribosome coverage gradually decreased in the vicinity of start codons and increased near stop codons, revealing complex age-related changes in the translation process. Taken together, our results reveal systematic and multidimensional deregulation of protein synthesis, showing how this major cellular process declines with age.


Assuntos
Envelhecimento/fisiologia , Regulação da Expressão Gênica/fisiologia , Biossíntese de Proteínas/fisiologia , Ribossomos/metabolismo , Animais , Códon de Iniciação/metabolismo , Biologia Computacional , Masculino , Camundongos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA-Seq , Ribossomos/genética , Transdução de Sinais/fisiologia , Serina-Treonina Quinases TOR/metabolismo , Transcriptoma/fisiologia
5.
PLoS Genet ; 15(2): e1007917, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30707697

RESUMO

Hbs1 has been established as a central component of the cell's translational quality control pathways in both yeast and prokaryotic models; however, the functional characteristics of its human ortholog (Hbs1L) have not been well-defined. We recently reported a novel human phenotype resulting from a mutation in the critical coding region of the HBS1L gene characterized by facial dysmorphism, severe growth restriction, axial hypotonia, global developmental delay and retinal pigmentary deposits. Here we further characterize downstream effects of the human HBS1L mutation. HBS1L has three transcripts in humans, and RT-PCR demonstrated reduced mRNA levels corresponding with transcripts V1 and V2 whereas V3 expression was unchanged. Western blot analyses revealed Hbs1L protein was absent in the patient cells. Additionally, polysome profiling revealed an abnormal aggregation of 80S monosomes in patient cells under baseline conditions. RNA and ribosomal sequencing demonstrated an increased translation efficiency of ribosomal RNA in Hbs1L-deficient fibroblasts, suggesting that there may be a compensatory increase in ribosome translation to accommodate the increased 80S monosome levels. This enhanced translation was accompanied by upregulation of mTOR and 4-EBP protein expression, suggesting an mTOR-dependent phenomenon. Furthermore, lack of Hbs1L caused depletion of Pelota protein in both patient cells and mouse tissues, while PELO mRNA levels were unaffected. Inhibition of proteasomal function partially restored Pelota expression in human Hbs1L-deficient cells. We also describe a mouse model harboring a knockdown mutation in the murine Hbs1l gene that shared several of the phenotypic elements observed in the Hbs1L-deficient human including facial dysmorphism, growth restriction and retinal deposits. The Hbs1lKO mice similarly demonstrate diminished Pelota levels that were rescued by proteasome inhibition.


Assuntos
Proteínas de Ligação ao GTP/genética , Mamíferos/genética , Proteínas dos Microfilamentos/genética , Monossomia/genética , Animais , Linhagem Celular , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Mutação/genética , Fenótipo , Polirribossomos/genética , Complexo de Endopeptidases do Proteassoma/genética , RNA/genética , RNA Mensageiro/genética , Ribossomos/genética , Serina-Treonina Quinases TOR/genética , Regulação para Cima/genética
6.
Nucleic Acids Res ; 45(2): e6, 2017 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-27638886

RESUMO

Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is important to ensure both stability of ribosomal particles and complete conversion of polysomes to monosomes. We performed comparative ribosome profiling in yeast and mice with various ribonucleases including I, A, S7 and T1, characterized their cutting preferences, trinucleotide periodicity patterns and coverage similarities across coding sequences, and showed that they yield comparable estimations of gene expression when ribosome integrity is not compromised. However, ribosome coverage patterns of individual transcripts had little in common between the ribonucleases. We further examined their potency at converting polysomes to monosomes across other commonly used model organisms, including bacteria, nematodes and fruit flies. In some cases, ribonuclease treatment completely degraded ribosome populations. Ribonuclease T1 was the only enzyme that preserved ribosomal integrity while thoroughly converting polysomes to monosomes in all examined species. This study provides a guide for ribonuclease selection in ribosome profiling experiments across most common model systems.


Assuntos
Biossíntese de Proteínas , Ribonucleases/metabolismo , Ribossomos/metabolismo , Animais , Drosophila/genética , Drosophila/metabolismo , Helmintos/genética , Helmintos/metabolismo , Camundongos , Estabilidade Proteica , Estabilidade de RNA , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo
7.
Proc Natl Acad Sci U S A ; 112(34): 10685-90, 2015 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-26261310

RESUMO

Aerobic respiration is a fundamental energy-generating process; however, there is cost associated with living in an oxygen-rich environment, because partially reduced oxygen species can damage cellular components. Organisms evolved enzymes that alleviate this damage and protect the intracellular milieu, most notably thiol peroxidases, which are abundant and conserved enzymes that mediate hydrogen peroxide signaling and act as the first line of defense against oxidants in nearly all living organisms. Deletion of all eight thiol peroxidase genes in yeast (∆8 strain) is not lethal, but results in slow growth and a high mutation rate. Here we characterized mechanisms that allow yeast cells to survive under conditions of thiol peroxidase deficiency. Two independent ∆8 strains increased mitochondrial content, altered mitochondrial distribution, and became dependent on respiration for growth but they were not hypersensitive to H2O2. In addition, both strains independently acquired a second copy of chromosome XI and increased expression of genes encoded by it. Survival of ∆8 cells was dependent on mitochondrial cytochrome-c peroxidase (CCP1) and UTH1, present on chromosome XI. Coexpression of these genes in ∆8 cells led to the elimination of the extra copy of chromosome XI and improved cell growth, whereas deletion of either gene was lethal. Thus, thiol peroxidase deficiency requires dosage compensation of CCP1 and UTH1 via chromosome XI aneuploidy, wherein these proteins support hydroperoxide removal with the reducing equivalents generated by the electron transport chain. To our knowledge, this is the first evidence of adaptive aneuploidy counteracting oxidative stress.


Assuntos
Adaptação Fisiológica/genética , Aneuploidia , Deleção Cromossômica , Cromossomos Fúngicos/genética , Transporte de Elétrons/fisiologia , Proteínas Mitocondriais/fisiologia , Proteínas de Saccharomyces cerevisiae/fisiologia , Saccharomyces cerevisiae/fisiologia , Antimicina A/farmacologia , Citocromo-c Peroxidase/genética , Citocromo-c Peroxidase/fisiologia , Deleção de Genes , Dosagem de Genes , Genes Fúngicos , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/fisiologia , Peróxido de Hidrogênio/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/fisiologia , Proteínas Mitocondriais/genética , Oligomicinas/farmacologia , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/genética , Oxirredutases atuantes sobre Doadores de Grupo Enxofre/fisiologia , Peroxidases/deficiência , Peroxidases/genética , Espécies Reativas de Oxigênio/metabolismo , Rotenona/farmacologia , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/enzimologia , Proteínas de Saccharomyces cerevisiae/genética
8.
PLoS Genet ; 10(1): e1004019, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24391512

RESUMO

Cells respond to accumulation of misfolded proteins in the endoplasmic reticulum (ER) by activating the unfolded protein response (UPR) signaling pathway. The UPR restores ER homeostasis by degrading misfolded proteins, inhibiting translation, and increasing expression of chaperones that enhance ER protein folding capacity. Although ER stress and protein aggregation have been implicated in aging, the role of UPR signaling in regulating lifespan remains unknown. Here we show that deletion of several UPR target genes significantly increases replicative lifespan in yeast. This extended lifespan depends on a functional ER stress sensor protein, Ire1p, and is associated with constitutive activation of upstream UPR signaling. We applied ribosome profiling coupled with next generation sequencing to quantitatively examine translational changes associated with increased UPR activity and identified a set of stress response factors up-regulated in the long-lived mutants. Besides known UPR targets, we uncovered up-regulation of components of the cell wall and genes involved in cell wall biogenesis that confer resistance to multiple stresses. These findings demonstrate that the UPR is an important determinant of lifespan that governs ER stress and identify a signaling network that couples stress resistance to longevity.


Assuntos
Estresse do Retículo Endoplasmático/genética , Longevidade/genética , Dobramento de Proteína , Resposta a Proteínas não Dobradas/genética , Envelhecimento/genética , Retículo Endoplasmático/genética , Humanos , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Mutação , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Ribossomos/genética , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Via Secretória/genética , Transdução de Sinais/genética
9.
Nucleic Acids Res ; 42(17): e134, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25056308

RESUMO

Ribosome profiling and high-throughput sequencing provide unprecedented opportunities for the analysis of mRNA translation. Using this novel method, several studies have demonstrated the widespread role of short upstream reading frames in translational control as well as slower elongation at the beginning of open reading frames in response to stress. Based on the initial studies, the importance of adding or omitting translation inhibitors, such as cycloheximide, was noted as it markedly affected ribosome coverage profiles. For that reason, many recent studies omitted translation inhibitors in the culture medium. Here, we investigate the influence of ranging cycloheximide concentrations on ribosome profiles in Saccharomyces cerevisiae and demonstrate that increasing the drug concentration can overcome some of the artifacts. We subjected cells to various manipulations and show that neither oxidative stress nor heat shock nor amino acid starvation affect translation elongation. Instead, the observations in the initial studies are the result of cycloheximide-inflicted artifacts. Likewise, we find little support for short upstream reading frames to be involved in widespread protein synthesis regulation under stress conditions. Our study highlights the need for better standardization of ribosome profiling methods.


Assuntos
Cicloeximida/farmacologia , Inibidores da Síntese de Proteínas/farmacologia , Ribossomos/efeitos dos fármacos , Artefatos , Resposta ao Choque Térmico/genética , Sequenciamento de Nucleotídeos em Larga Escala , Estresse Oxidativo/genética , Biossíntese de Proteínas/efeitos dos fármacos , RNA Mensageiro/química , RNA Mensageiro/metabolismo , Ribossomos/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Análise de Sequência de RNA
10.
Proc Natl Acad Sci U S A ; 109(43): 17394-9, 2012 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-23045643

RESUMO

Information on unique and coordinated regulation of transcription and translation in response to stress is central to the understanding of cellular homeostasis. Here we used ribosome profiling coupled with next-generation sequencing to examine the interplay between transcription and translation under conditions of hydrogen peroxide treatment in Saccharomyces cerevisiae. Hydrogen peroxide treatment led to a massive and rapid increase in ribosome occupancy of short upstream ORFs, including those with non-AUG translational starts, and of the N-terminal regions of ORFs that preceded the transcriptional response. In addition, this treatment induced the synthesis of N-terminally extended proteins and elevated stop codon read-through and frameshift events. It also increased ribosome occupancy at the beginning of ORFs and potentially the duration of the elongation step. We identified proteins whose synthesis was regulated rapidly by hydrogen peroxide posttranscriptionally; however, for the majority of genes increased protein synthesis followed transcriptional regulation. These data define the landscape of genome-wide regulation of translation in response to hydrogen peroxide and suggest that potentiation (coregulation of the transcript level and translation) is a feature of oxidative stress.


Assuntos
Genoma Fúngico , Estresse Oxidativo , Biossíntese de Proteínas , Ribossomos , Códon de Iniciação , Códon de Terminação , Peróxido de Hidrogênio/farmacologia , Fases de Leitura Aberta , Saccharomyces cerevisiae/efeitos dos fármacos , Transcrição Gênica
11.
Nat Commun ; 13(1): 355, 2022 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-35039495

RESUMO

The naked mole-rat (NMR) is an exceptionally long-lived rodent that shows no increase of mortality with age, defining it as a demographically non-aging mammal. Here, we perform bisulfite sequencing of the blood of > 100 NMRs, assessing > 3 million common CpG sites. Unsupervised clustering based on sites whose methylation correlates with age reveals an age-related methylome remodeling, and we also observe a methylome information loss, suggesting that NMRs age. We develop an epigenetic aging clock that accurately predicts the NMR age. We show that these animals age much slower than mice and much faster than humans, consistent with their known maximum lifespans. Interestingly, patterns of age-related changes of clock sites in Tert and Prpf19 differ between NMRs and mice, but there are also sites conserved between the two species. Together, the data indicate that NMRs, like other mammals, epigenetically age even in the absence of demographic aging of this species.


Assuntos
Envelhecimento/genética , Epigênese Genética , Ratos-Toupeira/crescimento & desenvolvimento , Ratos-Toupeira/genética , Envelhecimento/sangue , Animais , Relógios Biológicos/genética , Ilhas de CpG/genética , Metilação de DNA/genética , Demografia , Regulação da Expressão Gênica , Humanos , Camundongos , Ratos-Toupeira/sangue , Fatores de Processamento de RNA/genética , Fatores de Processamento de RNA/metabolismo , Telomerase/genética , Telomerase/metabolismo
12.
J Biol Chem ; 285(7): 4595-602, 2010 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-20018845

RESUMO

Mammalian cytosolic and mitochondrial thioredoxin reductases are essential selenocysteine-containing enzymes that control thioredoxin functions. Thioredoxin/glutathione reductase (TGR) is a third member of this enzyme family. It has an additional glutaredoxin domain and shows highest expression in testes. Herein, we found that human and several other mammalian TGR genes lack any AUG codons that could function in translation initiation. Although mouse and rat TGRs have such codons, we detected protein sequences upstream of them by immunoblot assays and direct proteomic analyses. Further gene engineering and expression analyses demonstrated that a CUG codon, located upstream of the sequences previously thought to initiate translation, is the actual start codon in mouse TGR. The use of this codon relies on the Kozak consensus sequence and ribosome-scanning mechanism. However, CUG serves as an inefficient start codon that allows downstream initiation, thus generating two isoforms of the enzyme in vivo and in vitro. The use of CUG evolved in mammalian TGRs, and in some of these organisms, GUG is used instead. The newly discovered longer TGR form shows cytosolic localization in cultured cells and is expressed in spermatids in mouse testes. This study shows that CUG codon is used as an inefficient start codon to generate protein isoforms in mouse.


Assuntos
Isoenzimas/genética , Complexos Multienzimáticos/genética , NADH NADPH Oxirredutases/genética , Testículo/enzimologia , Sequência de Aminoácidos , Animais , Células COS , Linhagem Celular , Chlorocebus aethiops , Códon de Iniciação/genética , Códon de Iniciação/fisiologia , Eletroforese em Gel de Poliacrilamida , Humanos , Imuno-Histoquímica , Técnicas In Vitro , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , NADH NADPH Oxirredutases/química , NADH NADPH Oxirredutases/metabolismo , Células NIH 3T3 , Ratos , Epitélio Seminífero/metabolismo , Homologia de Sequência de Aminoácidos
13.
Methods Mol Biol ; 2252: 239-248, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33765279

RESUMO

In the past 10 years, standard transcriptome sequencing protocols were optimized so well that no prior experience is required to prepare the sequencing library. Often, all enzymatic steps are designed to work in the same reaction tube minimizing handling time and reducing human errors. Ribosome profiling stands out from these methods. It is a very demanding technique that requires isolation of intact ribosomes, and thus there are multiple additional considerations that must be accounted for (McGlincy and Ingolia, Methods 126:112-129, 2017). In this chapter, we discuss how to select a ribonuclease to produce ribosomal footprints that will be later converted to the sequencing library. Several ribonucleases with different cutting patterns are commercially available. Selecting the right one for the experimental application can save a lot of time and frustration.


Assuntos
Ribonucleases/metabolismo , Ribossomos/metabolismo , Perfilação da Expressão Gênica/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Biossíntese de Proteínas , RNA Mensageiro/genética , Análise de Sequência de RNA/métodos
14.
Methods Mol Biol ; 2252: 189-200, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33765276

RESUMO

Modern methods of genome editing enable the rapid generation of mouse models to study the regulation of protein synthesis. At the same time, few options are available to study translation in rodents as the animal's complexity severely limits the repertoire of experimental tools. Here we describe a method to monitor translation in mice and other small animals. The technique is based on a ribosome profiling and specifically tailored toward measuring translation elongation. However, it can be easily applied for short upstream reading frames discovery. The advantage of this method is the ability to study translation in fully developed animals without extracting and subculturing cells, therefore, maintaining unperturbed physiological conditions.


Assuntos
Elongação Traducional da Cadeia Peptídica , Ribossomos/metabolismo , Análise de Sequência de RNA/métodos , Animais , Rim/metabolismo , Fígado/metabolismo , Camundongos , Músculo Esquelético/metabolismo , Especificidade de Órgãos , RNA Mensageiro/genética , Fases de Leitura
15.
Nat Commun ; 11(1): 588, 2020 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-32001709

RESUMO

Alteration of normal ploidy (aneuploidy) can have a number of opposing effects, such as unbalancing protein abundances and inhibiting cell growth but also accelerating genetic diversification and rapid adaptation. The interplay of these detrimental and beneficial effects remains puzzling. Here, to understand how cells develop tolerance to aneuploidy, we subject disomic (i.e. with an extra chromosome copy) strains of yeast to long-term experimental evolution under strong selection, by forcing disomy maintenance and daily population dilution. We characterize mutations, karyotype alterations and gene expression changes, and dissect the associated molecular strategies. Cells with different extra chromosomes accumulated mutations at distinct rates and displayed diverse adaptive events. They tended to evolve towards normal ploidy through chromosomal DNA loss and gene expression changes. We identify genes with recurrent mutations and altered expression in multiple lines, revealing a variant that improves growth under genotoxic stresses. These findings support rapid evolvability of disomic strains that can be used to characterize fitness effects of mutations under different stress conditions.


Assuntos
Adaptação Fisiológica/genética , Aneuploidia , Evolução Molecular , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/fisiologia , Alelos , Dano ao DNA , Dosagem de Genes , Regulação Fúngica da Expressão Gênica , Redes Reguladoras de Genes , Genoma Fúngico , Cariótipo , Mutação/genética , Fenótipo , Regiões Promotoras Genéticas/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Transcrição Gênica
16.
Aging Cell ; 19(10): e13230, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33006233

RESUMO

COVID-19 is an ongoing pandemic caused by the SARS-CoV-2 coronavirus that poses one of the greatest challenges to public health in recent years. SARS-CoV-2 is known to preferentially target older subjects and those with pre-existing conditions, but the reason for this age dependence is unclear. Here, we found that the case fatality rate for COVID-19 grows exponentially with age in all countries tested, with the doubling time approaching that of all-cause human mortality. In addition, men and those with multiple age-related diseases are characterized by increased mortality. Moreover, similar mortality patterns were found for all-cause pneumonia. We further report that the gene expression of ACE2, the SARS-CoV-2 receptor, grows in the lung with age, except for subjects on a ventilator. Together, our findings establish COVID-19 as an emergent disease of aging, and age and age-related diseases as its major risk factors. In turn, this suggests that COVID-19, and deadly respiratory diseases in general, may be targeted, in addition to antiviral approaches, by approaches that target the aging process.


Assuntos
Envelhecimento/imunologia , Infecções por Coronavirus/mortalidade , Pneumonia Viral/mortalidade , Fatores Etários , Idoso , Enzima de Conversão de Angiotensina 2 , Betacoronavirus , COVID-19 , Feminino , Saúde Global , Humanos , Masculino , Pandemias , Peptidil Dipeptidase A/metabolismo , SARS-CoV-2 , Fatores Sexuais
17.
Sci Adv ; 6(17): eaaz4370, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32494643

RESUMO

During stress, global translation is reduced, but specific transcripts are actively translated. How stress-responsive mRNAs are selectively translated is unknown. We show that METL-5 methylates adenosine 1717 on 18S ribosomal RNA in C. elegans, enhancing selective ribosomal binding and translation of specific mRNAs. One of these mRNAs, CYP-29A3, oxidizes the omega-3 polyunsaturated fatty acid eicosapentaenoic acid to eicosanoids, key stress signaling molecules. While metl-5-deficient animals grow normally under homeostatic conditions, they are resistant to a variety of stresses. metl-5 mutant worms also show reduced bioactive lipid eicosanoids and dietary supplementation of eicosanoid products of CYP-29A3 restores stress sensitivity of metl-5 mutant worms. Thus, methylation of a specific residue of 18S rRNA by METL-5 selectively enhances translation of cyp-29A3 to increase production of eicosanoids, and blocking this pathway increases stress resistance. This study suggests that ribosome methylation can facilitate selective translation, providing another layer of regulation of the stress response.

18.
Mol Ther Nucleic Acids ; 19: 252-266, 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-31855834

RESUMO

Translation is an essential biological process, and dysregulation is associated with a range of diseases including ribosomopathies, diabetes, and cancer. Here, we examine translation dysregulation in vivo using RNAi to knock down the m-subunit of the translation initiation factor eIF3 in the mouse liver. Transcriptome sequencing, ribosome profiling, whole proteome, and phosphoproteome analyses show that eIF3m deficiency leads to the transcriptional response and changes in cellular translation that yield few detectable differences in the translation of particular mRNAs. The transcriptional response fell into two main categories: ribosome biogenesis (increased transcription of ribosomal proteins) and cell metabolism (alterations in lipid, amino acid, nucleic acid, and drug metabolism). Analysis of ribosome biogenesis reveals inhibition of rRNA processing, highlighting decoupling of rRNA synthesis and ribosomal protein gene transcription in response to eIF3m knockdown. Interestingly, a similar reduction in eIF3m protein levels is associated with induction of the mTOR pathway in vitro but not in vivo. Overall, this work highlights the utility of a RNAi-based in vivo approach for studying the regulation of mammalian translation in vivo.

19.
Sci Rep ; 10(1): 15473, 2020 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-32968084

RESUMO

Due to breakthroughs in RNAi and genome editing methods in the past decade, it is now easier than ever to study fine details of protein synthesis in animal models. However, most of our understanding of translation comes from unicellular organisms and cultured mammalian cells. In this study, we demonstrate the feasibility of perturbing protein synthesis in a mouse liver by targeting translation elongation factor 2 (eEF2) with RNAi. We were able to achieve over 90% knockdown efficacy and maintain it for 2 weeks effectively slowing down the rate of translation elongation. As the total protein yield declined, both proteomics and ribosome profiling assays showed robust translational upregulation of ribosomal proteins relative to other proteins. Although all these genes bear the TOP regulatory motif, the branch of the mTOR pathway responsible for translation regulation was not activated. Paradoxically, coordinated translational upregulation of ribosomal proteins only occurred in the liver but not in murine cell culture. Thus, the upregulation of ribosomal transcripts likely occurred via passive mTOR-independent mechanisms. Impaired elongation sequesters ribosomes on mRNA and creates a shortage of free ribosomes. This leads to preferential translation of transcripts with high initiation rates such as ribosomal proteins. Furthermore, severe eEF2 shortage reduces the negative impact of positively charged amino acids frequent in ribosomal proteins on ribosome progression.


Assuntos
Quinase do Fator 2 de Elongação/metabolismo , Fígado/metabolismo , RNA Interferente Pequeno/metabolismo , Proteínas Ribossômicas/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Animais , Ciclo Celular , Feminino , Técnicas de Silenciamento de Genes , Camundongos , Biossíntese de Proteínas , Proteoma/metabolismo , RNA Mensageiro/metabolismo , Regulação para Cima
20.
Cell Biol Int ; 33(10): 1057-64, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19560548

RESUMO

The positioning of the nucleus is achieved by two interconnected processes, anchoring and migration, both of which are controlled by cytoskeleton structures. Rotation is a special type of nuclear motility in many cell types, but its significance remains unclear. We used a vimentin-null cell line, MFT-16, which shows extensive nuclear rotation to study the phenomenon in detail. By selective disruption of cytoskeletal structures and video-microscopic analysis, nuclear rotation was a microtubule-dependent process that F-actin partially impedes. The dynein-dynactin complex is responsible and inhibiting this motor by expression of a dominant negative mutant of its component P-150 completely stops it. Nuclear rotation is powered by dynein associated with the nuclear envelope along stationary microtubules, centrosomes remaining immobile. We confirmed that vimentin IFs inhibit nuclear rotation, and variant proteins of the mutated wild type gene for vimentin that lacked considerable fragments of the N- and C-terminal domains restored nuclear anchoring. Immunochemical analysis showed that these mutated IFs also bound plectin, arguing for a key role of this cytolinker protein in nuclear anchoring. It is proposed that this versatile machinery guarantees not only rotation and the correct location of a nucleus, but also its orientation in a cell.


Assuntos
Actinas/metabolismo , Núcleo Celular/metabolismo , Dineínas/metabolismo , Filamentos Intermediários/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Vimentina/metabolismo , Animais , Linhagem Celular , Complexo Dinactina , Humanos , Camundongos , Plectina/metabolismo , Transfecção , Vimentina/genética
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