Active Solid-State Nanopores: Self-Driven Flows/Chaos at the Liquid-Gas Nanofluidic Interface.

Here, we present a comprehensive study of self-driven flow dynamics at the liquid-gas interface within nanofluidic pores in the absence of external driving forces. The investigation focuses on the Rayleigh-Taylor instability phenomena that occur in sub-100 nm scale fluidic pores interfacing between 2 µm scale water and air reservoir. We obtain a flow velocity equation, and we validate it using simulations, concentrating on the mass transfer efficiency of these flow structures. Furthermore, we introduce the concept─"active solid-state nanopore"─that exhibits a self-driven flow switching behavior, transitioning between active and passive states without the need for mechanical components. We found a unique state of chaos at the nanoscale resembling the chaotic motion of fluid. This study contributes to the preliminary understanding of fluid dynamics at the classical-quantum interface. Implications of self-driven nanofluidics extend across diverse fields from biosensing and healthcare applications to advancing net-zero sustainable energy production and contributing to the fundamental understanding of fluid dynamics in confined spaces.

Atomic Defects Influenced Mechanics of II-VI Nanocrystals.

Mechanical properties of nanocrystals are influenced by atomic defects. Here, we demonstrate the effect of planar defects on the mechanics of ZnO nanorods using atomic force microscopy, high-resolution transmission electron microscopy, and large-scale atomistic simulation. We study two different conditionally grown single nanorods. One contains extended I1-type stacking fault (SF) and another is defect free. The SF containing nanorods show buckling behaviors with reduced critical loading, whereas the other kinds show linear elastic behavior. We also studied the size dependence of elastic modulus and yield strength. The elastic modulus in both nanorods is inversely proportional to their size. Similar trend is observed for yield strength in the SF containing nanorods; however, the opposite is observed in the SF-free nanorods. This first experimental and theoretical study will guide toward the development of reliable electromechanical devices.

Photoluminescence of carbon nanodots: dipole emission centers and electron-phonon coupling.

Inorganic carbon nanomaterials, also called carbon nanodots, exhibit a strong photoluminescence with unusual properties and, thus, have been the focus of intense research. Nonetheless, the origin of their photoluminescence is still unclear and the subject of scientific debates. Here, we present a single particle comprehensive study of carbon nanodot photoluminescence, which combines emission and lifetime spectroscopy, defocused emission dipole imaging, azimuthally polarized excitation dipole scanning, nanocavity-based quantum yield measurements, high resolution transmission electron microscopy, and atomic force microscopy. We find that photoluminescent carbon nanodots behave as electric dipoles, both in absorption and emission, and that their emission originates from the recombination of photogenerated charges on defect centers involving a strong coupling between the electronic transition and collective vibrations of the lattice structure.

Exosomal PTEN as a Predictive Marker of Aggressive Gliomas.

Background: Liquid biopsies have emerged as convenient alternative diagnostic methods to invasive biopsies, by evaluating disease-specific biomarkers and monitoring the disease risk noninvasively. Phosphatase and tensin homolog deleted in chromosome 10 (PTEN) is a potent tumor suppressor, and its deletion/mutations are common in gliomas. Objective: Evaluate the feasibility of non-invasive detection of PTEN and its downstream genes in serum exosomes of glioma patients. Materials and methods: PTEN, Yes-associated-protein 1 (YAP1), and lysyl oxidase (LOX) transcript expression were monitored through polymerase chain reaction (PCR) in serum exosomes and their paired tumor tissues. The impact of PTEN and its axis genes expression on the overall survival (OS) was monitored. Results: Out of the 106 glioma serum samples evaluated, PTEN was retained/lost in 65.4%/34.6% of the tumor samples while it was retained/lost in 67.1%/32.9% of their paired exosomal fractions. PTEN expression in both tissue and paired exosomal fractions was observed in 48.11% of the samples. Sanger sequencing detected three mutations (Chr10: 89720791(A>G), Chr10:89720749(C>T), and Chr10:89720850(A>G). Both PTEN-responsive downstream genes (YAP1) and LOX axis were upregulated in the PTEN-deficient samples. PTEN loss was associated with poor survival in the glioma patients (hazard ratio (HR) 0.68, confidence interval (CI): 0.35-1.31, P = 0.28). The OS of the exosomal PTEN cohort coincided with the tumor-tissue PTEN devoid group (HR 1.08, CI: 0.49-2.36, P = 0.85). While, old age yielded the worst prognosis; gender, location, and grade were not prognostic of OS in the multivariate analysis. Conclusions: PTEN and its responsive genes YAP1 and LOX can be detected in serum exosomes and can serve as essential tools for the non-invasive evaluation/identification of aggressive gliomas.

Single-molecule confinement with uniform electrodynamic nanofluidics.

To date, we could not engineer Nature's ability to dynamically handle diffusing single molecules in the liquid-phase as it takes place in pore-forming proteins and tunnelling nanotubes. Consistent handling of individual single molecules in a liquid is of paramount importance to fundamental molecular studies and technological benefits, like single-molecule level separation and sorting for early biomedical diagnostics, microscopic studies of molecular interactions and electron/optical microscopy of molecules and nanomaterials. We can consistently resolve the dynamics of diffusing single molecules if they are confined within a uniform dielectric environment at nanometre length-scales. A uniform dielectric environment is the key characteristic since intrinsic electronic properties of molecules were modified while interacting with any surfaces, and the effect is not the same from one dielectric surface to another. We present dynamic nanofluidic detection of optically active single molecules in a liquid. An all-silica nanofluidic environment was used to electrokinetically handle individual single-molecules where molecular shot noise was resolved. We recorded the single-molecule motion of small fragments of DNA, carbon-nanodots, and organic fluorophores in water. The electrokinetic 1D molecular mass transport under two-focus fluorescence correlation spectroscopy (2fFCS) showed confinement-induced modified molecular interactions (due to various inter-molecular repulsive and attractive forces), which have been theoretically interpreted as molecular shot noise. Our demonstration of high-throughput nanochannel fabrication, 2fFCS-based 1D confined detection of fast-moving single molecules and fundamental understanding of molecular shot noise may open an avenue for single-molecule experiments where physical manipulation of dynamics is necessary.

Computer-assisted delineation of cerebral infarct from diffusion-weighted MRI using Gaussian mixture model.

PURPOSE: Diffusion-weighted imaging (DWI) is a widely used medical imaging modality for diagnosis and monitoring of cerebral stroke. The identification of exact location of stroke lesion helps in perceiving its characteristics, an essential part of diagnosis and treatment planning. This task is challenging due to the typical shape of the stroke lesion. This paper proposes an efficient method for computer-aided delineation of stroke lesions from DWI images. METHOD: Proposed methodology comprises of three steps. At the initial step, image contrast has been improved by applying fuzzy intensifier leading to the better visual quality of the stroke lesion. In the following step, a two-class (stroke lesion area vs. non-stroke lesion area) segmentation technique based on Gaussian mixture model has been designed for the localization of stroke lesion. To eliminate the artifacts which would appear during segmentation process, a binary morphological post-processing through area operator has been defined for exact delineation of the lesion area. RESULT: The performance of the proposed methodology has been compared with the manually delineated images (ground truth) obtained from different experts, individually. Quantitative evaluation with respect to various performance measures (such as dice coefficient, Jaccard score, and correlation coefficient) shows the efficient performance of the proposed technique.

Single-photon-multi-layer-interference lithography for high-aspect-ratio and three-dimensional SU-8 micro-/nanostructures.

We report microstructures of SU-8 photo-sensitive polymer with high-aspect-ratio, which is defined as the ratio of height to in-plane feature size. The highest aspect ratio achieved in this work exceeds 250. A multi-layer and single-photon lithography approach is used in this work to expose SU-8 photoresist of thickness up to 100 µm. Here, multi-layer and time-lapsed writing is the key concept that enables nanometer localised controlled photo-induced polymerisation. We use a converging monochromatic laser beam of 405 nm wavelength with a controllable aperture. The reflection of the converging optics from the silicon substrate underneath is responsible for a trapezoidal edge profile of SU-8 microstructure. The reflection induced interfered point-spread-function and multi-layer-single-photon exposure helps to achieve sub-wavelength feature sizes. We obtained a 75 nm tip diameter on a pyramid shaped microstructure. The converging beam profile determines the number of multiple optical focal planes along the depth of field. These focal planes are scanned and exposed non-concurrently with varying energy dosage. It is notable that an un-automated height axis control is sufficient for this method. All of these contribute to realising super-high-aspect-ratio and 3D micro-/nanostructures using SU-8. Finally, we also address the critical problems of photoresist-based micro-/nanofabrication and their solutions.

Detection of quantum well induced single degenerate-transition-dipoles in ZnO nanorods.

Quantifying and characterising atomic defects in nanocrystals is difficult and low-throughput using the existing methods such as high resolution transmission electron microscopy (HRTEM). In this article, using a defocused wide-field optical imaging technique, we demonstrate that a single ultrahigh-piezoelectric ZnO nanorod contains a single defect site. We model the observed dipole-emission patterns from optical imaging with a multi-dimensional dipole and find that the experimentally observed dipole pattern and model-calculated patterns are in excellent agreement. This agreement suggests the presence of vertically oriented degenerate-transition-dipoles in vertically aligned ZnO nanorods. The HRTEM of the ZnO nanorod shows the presence of a stacking fault, which generates a localised quantum well induced degenerate-transition-dipole. Finally, we elucidate that defocused wide-field imaging can be widely used to characterise defects in nanomaterials to answer many difficult questions concerning the performance of low-dimensional devices, such as in energy harvesting, advanced metal-oxide-semiconductor storage, and nanoelectromechanical and nanophotonic devices.

Elucidation of procoagulant mechanism and pathophysiological significance of a new prothrombin activating metalloprotease purified from Daboia russelii russelii venom.

The procoagulant proteases present in Russell's Viper venom (RVV) are responsible for promoting consumption coagulopathy in victims. In this study, a procoagulant metalloprotease (Rusviprotease) possessing prothrombin activating and α-fibrinogenase properties has been purified and characterized from RVV. Rusviprotease is a 26.8 kDa glycoprotein which also exists in other multimeric forms. The peptide mass fingerprinting and secondary structure analyses of Rusviprotease revealed its similarity with snake venom prothrombin activators and metalloproteases. Similar to group A prothrombin activators, Rusviprotease cleaved prothrombin independent of any co-factor requirement generating meizothrombin which is further cleaved to form thrombin. The Km and Vmax values of Rusviprotease towards prothrombin were determined to be 1.73 µM, and 153.5 nM thrombin generated/min/µmoles of Rusviprotease, respectively. The Km and Vmax values of Rusviprotease towards fibrinogen were calculated to be 3.14 µM and 78.7 nmol/min, respectively. Spectrofluorometric study provided the evidence of interaction between Rusviprotease and factor Xa with a Kd value of 6.64 nM. This interaction augmented the prothrombin activating property of the factor Xa-prothrombinase-Rusviprotease complex by 2.5 fold. Intravenous injection of Rusviprotease to BALB/c mice (0.1 mg/kg) resulted in in vivo defibrinogenation rendering the blood incoagulable. In conclusion, Rusviprotease is the first example of a prothrombin activator with fibrinogenolytic property purified from Daboia russelii russelii venom.

Investigation of techniques for the measurement of articular cartilage surface roughness.

Articular cartilage is the bearing surface of synovial joints and plays a crucial role in the tribology to enable low friction joint movement. A detailed understanding of the surface roughness of articular cartilage is important to understand how natural joints behave and the parameters required for future joint replacement materials. Bovine articular cartilage on bone samples was prepared and the surface roughness was measured using scanning electron microscopy stereoscopic imaging at magnifications in the range 500× to 2000×. The surface roughness (two-dimensional, R(a), and three-dimensional, S(a)) of each sample was then measured using atomic force microscopy (AFM). For stereoscopic imaging the surface roughness was found to linearly increase with increasing magnification. At a magnification of 500× the mean surface roughness, R(a), was in the range 165.4±5.2 nm to 174±39.3 nm; total surface roughness S(a) was in the range 183-261 nm. The surface roughness measurements made using AFM showed R(a) in the range 82.6±4.6 nm to 114.4±44.9 nm and S(a) in the range 86-136 nm. Values obtained using SEM stereo imaging were always larger than those obtained using AFM. Stereoscopic imaging can be used to investigate the surface roughness of articular cartilage. The variations seen between measurement techniques show that when making comparisons between the surface roughness of articular cartilage it is important that the same technique is used.