Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5'-monophosphate as the biological substrate.

Eukaryotic life benefits from-and ofttimes critically relies upon-the de novo biosynthesis and supply of vitamins and micronutrients from bacteria. The micronutrient queuosine (Q), derived from diet and/or the gut microbiome, is used as a source of the nucleobase queuine, which once incorporated into the anticodon of tRNA contributes to translational efficiency and accuracy. Here, we report high-resolution, substrate-bound crystal structures of the Sphaerobacter thermophilus queuine salvage protein Qng1 (formerly DUF2419) and of its human ortholog QNG1 (C9orf64), which together with biochemical and genetic evidence demonstrate its function as the hydrolase releasing queuine from queuosine-5'-monophosphate as the biological substrate. We also show that QNG1 is highly expressed in the liver, with implications for Q salvage and recycling. The essential role of this family of hydrolases in supplying queuine in eukaryotes places it at the nexus of numerous (patho)physiological processes associated with queuine deficiency, including altered metabolism, proliferation, differentiation and cancer progression.

The Effects of Low-Load Squat Jump and Maximal Isometric Priming Exercise on Muscular Performance and Perceptual State.

ABSTRACT: Harrison, PW, James, LP, Jenkins, DG, McGuigan, MR, Holmberg, PM, and Kelly, VG. The effects of low-load squat jump and maximal isometric priming exercise on muscular performance and perceptual state. J Strength Cond Res 38(1): 1-9, 2024-The aim of this study was to examine responses at 3 and 27 hours after low-load jump squat (LL) and maximal isometric half-squat (ISO) priming stimuli. Fifteen resistance-trained males performed LL (4 × 3 at 20% 1 repetition maximum [1RM]), ISO (4 × 3 seconds), and control (CON) activities (standardized warm-up) in a randomized and counterbalanced order. Countermovement jump (CMJ) and isometric midthigh pull tests were conducted to assess performance changes after priming and CON activities. No clear changes in CMJ measures were found after priming activities compared with CON. However, small effect size improvements were found after priming stimuli completed on the same day. A 2.9% decrease in concentric phase duration (CI = 0.3-5.9, p = 0.333, Cliff's delta = -0.156) and a 9.1% increase in RSImod (CI = 0.2-12.3, p = 0.151, Cliff's delta = -0.218) occurred at 3 hours after LL compared with CON. Braking phase duration (CI = 0.8-10.6, p = 0.333, Cliff's delta = -0.213) was 2.9% shorter at 3 hours after ISO compared with CON. No clear changes in isometric peak force occurred after priming activities compared with CON. Additionally, questionnaires were completed to assess perceptual state and perceived effectiveness of the priming stimulus to influence performance. An increase in the "effect of activity" was perceived at 3 hours after LL and ISO (p = 0.013-0.044, Cliff's delta = 0.578-0.6) and at 27 hours after ISO (p = 0.99, Cliff's delta = 0.173) compared with CON. An increase in "muscular heaviness" was also reported at 3 hours after ISO compared with CON (p = 0.199, Cliff's delta = 0.320). The collective findings suggest limited benefits over the day after LL and ISO priming stimuli. However, as there was substantial variation in individual responses, the relative nature of priming responses should be considered when prescribing similar strategies in practical environments.

Seasonal Muscular Power Changes: Considerations of Concurrent Resistance and Field-Based Training in Professional Rugby League.

ABSTRACT: Redman, KJ, Wade, L, Whitley, R, Connick, MJ, Kelly, VG, and Beckman, EM. Seasonal muscular power changes: considerations of concurrent resistance and field-based training in professional rugby league. J Strength Cond Res XX(X): 000-000, 2024-A rugby league player's ability to develop or maintain lower-body power will be significantly influenced by the resistance and field-based training loads completed. This study aimed to examine the power changes across a rugby league season and to investigate the relationship of concurrent training and training experience on power changes. Eighteen rugby league players participated in this study. Lower-body power was evaluated using a countermovement jump throughout the season. Four measures of external training loads were collected. A Friedman's test was used to assess differences in power throughout different phases of the season. A series of Spearman's rank-order correlations were conducted to assess the relationship between percentage changes in power during the training block, external training loads, and training experience. Countermovement jump peak velocity significantly decreased from the start (p = 0.006) and end of pre-season (p = 0.022) to the late competition. Correlation analysis revealed a large significant association between early competition field-based external loads (relative (rs = -0.571) and total distance (rs = -0.628)) and change in peak power. Very large significant negative relationships were also observed between mid-competition volume load with change in peak force (rs = -0.825) and peak power (rs = -0.736). Training experience was not significantly associated with changes in muscular power variables throughout this study. Coaches should seek to monitor changes in peak power when competition begins and to assist with decision-making for potential adjustments to running volume and intensity during field-based sessions.

Effects of Repeated Jump Testing and Diurnal Changes on Subsequent Countermovement Jump and Squat Jump Output and Force-Time Characteristics.

ABSTRACT: Harrison, PW, James, LP, Jenkins, DG, Holmberg, PM, and Kelly, VG. Effects of repeated jump testing and diurnal changes on subsequent countermovement jump and squat jump output and force-time characteristics. J Strength Cond Res 38(1): 174-179, 2024-The aim of this brief study was to investigate the effects of repeated jump testing on performance over 2 consecutive days while considering the possibility of diurnal changes. Fourteen male subjects and 14 recreationally active female subjects completed countermovement jump (CMJ) and squat jump (SJ) testing on 5 occasions (baseline [0,800], 5 minutes [0,820], 8 hours [1,600], 24 hours [0,800], and 32 hours [1,600]) over 32 hours. An additional rested baseline test was conducted on a separate day in the afternoon (1,600) to compare jump performance between morning and afternoon baseline values. Excluding small decreases in CMJ height at 24 hours (p = 0.292, Cliff's delta = -0.225) in male subjects and similar decreases in CMJ height at 5 minutes (p = 0.034, Cliff's delta = -0.245) in addition to SJ height:contraction time at 32 hours (p = 0.126, Cliff's delta = 0.153) in female subjects, findings generally showed no changes in jump performance over multiple assessments. Squat jump metrics may have showed small improvements between morning and afternoon baseline values in male subjects (SJ height:contraction time [p = 0.030, Cliff's delta = 0.225]) and female subjects (SJ height [p = 0.013, Cliff's delta = 0.173] and SJ height:contraction time [p = 0.091, Cliff's delta = 0.163)]. As jump performance was largely unaffected by repeated jump testing, the present findings support the use of monitoring practices and research designs that require multiple jump assessments within acute periods (∼32 hours).

The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.

Base-modification can occur throughout a transfer RNA molecule; however, elaboration is particularly prevalent at position 34 of the anticodon loop (the wobble position), where it functions to influence protein translation. Previously, we demonstrated that the queuosine modification at position 34 can be substituted with an artificial analogue via the queuine tRNA ribosyltransferase enzyme to induce disease recovery in an animal model of multiple sclerosis. Here, we demonstrate that the human enzyme can recognize a very broad range of artificial 7-deazaguanine derivatives for transfer RNA incorporation. By contrast, the enzyme displays strict specificity for transfer RNA species decoding the dual synonymous NAU/C codons, determined using a novel enzyme-RNA capture-release method. Our data highlight the broad scope and therapeutic potential of exploiting the queuosine incorporation pathway to intentionally engineer chemical diversity into the transfer RNA anticodon.

Does Moderate-Load Priming Activity Influence Maximal Upper-Body Performance and Perceptual State?

ABSTRACT: Harrison, PW, Kelly, VG, Jenkins, DG, McGuigan, MR, Holmberg, PM, and James, LP. Does moderate-load priming activity influence maximal upper-body performance and perceptual state?. J Strength Cond Res 37(11): e581-e587, 2023-The results of previous research indicate that resistance exercise "priming" may improve strength-power measures within 48 hours after their completion. Although researchers have primarily examined performance responses after lower-body priming stimuli, investigations examining the effects of upper-body resistance priming exercises are presently limited. Therefore, the aim of this study was to examine upper-body pushing and pulling performance in addition to perceptual responses 3 and 27 hours after moderate-load (ML) upper-body resistance priming exercise. Fourteen resistance-trained men were assigned to complete ML priming (4 × 3 bench press and bench pull at 65% 1RM [repetition maximum]) and control (rest) protocols in a randomized and counterbalanced order. Peak velocity during the bench throw and bench pull tests involving different loads (25, 50, and 75% 1RM) showed no practical change at 3 and 27 hours after the priming session (p = 0.216-0.99, Cliff's d = -0.041 to 0.225). Small effect size increases in perceptual measures ("physical feeling," "physical performance," "aggression" [p = 0.400-0.553, Cliff's d = 0.183-0.201], and "muscular heaviness" [p = 0.178, Cliff's d = 0.231]) were found at 3 hours postpriming. A moderate practical increase was observed in perceived "physical feeling" compared with control (p = 0.385, Cliff's d = 0.349) in addition to small effect size increases in perceived "physical performance" and "aggression" (Cliff's d = 0.243-0.290) at 27 hours after priming activities. These results indicate that upper-body strength-power changes within 27 hours after ML upper-body resistance exercise priming are not practically meaningful.

CRISPR/Cas9-mediated SARM1 knockout and epitope-tagged mice reveal that SARM1 does not regulate nuclear transcription, but is expressed in macrophages.

SARM1 is a toll/interleukin-1 receptor -domain containing protein, with roles proposed in both innate immunity and neuronal degeneration. Murine SARM1 has been reported to regulate the transcription of chemokines in both neurons and macrophages; however, the extent to which SARM1 contributes to transcription regulation remains to be fully understood. Here, we identify differential gene expression in bone-marrow-derived macrophages (BMDMs) from C57BL/6 congenic 129 ES cell-derived Sarm1-/- mice compared with wild type (WT). However, we found that passenger genes, which are derived from the 129 donor strain of mice that flank the Sarm1 locus, confound interpretation of the results, since many of the identified differentially regulated genes come from this region. To re-examine the transcriptional role of SARM1 in the absence of passenger genes, here we generated three Sarm1-/- mice using CRISPR/Cas9. Treatment of neurons from these mice with vincristine, a chemotherapeutic drug causing axonal degeneration, confirmed SARM1's function in that process; however, these mice also showed that lack of SARM1 has no impact on transcription of genes previously shown to be affected such as chemokines. To gain further insight into SARM1 function, we generated an epitope-tagged SARM1 mouse. In these mice, we observed high SARM1 protein expression in the brain and brainstem and lower but detectable levels in macrophages. Overall, the generation of these SARM1 knockout and epitope-tagged mice has clarified that SARM1 is expressed in mouse macrophages yet has no general role in macrophage transcriptional regulation and has provided important new models to further explore SARM1 function.

De-novo designed ß-lysine derivatives can both augment and diminish the proliferation rates of E. coli through the action of Elongation Factor P.

An investigation into the effect of modified ß-lysines on the growth rates of eubacterial cells is reported. It is shown that the effects observed are due to the post translational modification of Elongation Factor P (EFP) with these compounds catalysed by PoxA. PoxA was found to be remarkably promiscuous, which allowed the activity of a wide range of exogenous ß-lysines to be examined. Two chain-elongated ß-lysine derivatives which differ in aminoalkyl chain length by only 2 carbon units exhibited opposing biological activities - one promoting growth and the other retarding it. Both compounds were shown to operate through modification of EFP.

Altered countermovement jump force profile and muscle-tendon unit kinematics following combined ballistic training.

Combined heavy- and light-load ballistic training is often employed in high-performance sport to improve athletic performance and is accompanied by adaptations in muscle architecture. However, little is known about how training affects muscle-tendon unit (MTU) kinematics during the execution of a sport-specific skill (e.g., jumping), which could improve our understanding of how training improves athletic performance. The aim of this study was to investigate vastus lateralis (VL) MTU kinematics during a countermovement jump (CMJ) following combined ballistic training. Eighteen young, healthy males completed a 10-week program consisting of weightlifting derivatives, plyometrics, and ballistic tasks under a range of loads. Ultrasonography of VL and force plate measurements during a CMJ were taken at baseline, mid-test, and post-test. The training program improved CMJ height by 11 ± 13%. During the CMJ, VL's MTU and series elastic element (SEE) length changes and velocities increased from baseline to post-test, but VL's fascicle length change and velocity did not significantly change. It is speculated that altered lower limb coordination and increased force output of the lower limb muscles during the CMJ allowed more energy to be stored within VL's SEE. This may have contributed to enhanced VL MTU work during the propulsion phase and an improved CMJ performance following combined ballistic training.

Relationship Between Preseason Training Load, Match Performance, and Match Activities in Professional Rugby League.

ABSTRACT: Crang, ZL, Hewitt, A, Scott, TJ, Kelly, VG, and Johnston, RD. Relationship between pre-season training load, match performance and match activities in professional rugby league. J Strength Cond Res 36(9): 2581-2588, 2022-This study aimed to establish the relationship between preseason training loads, technical match performance, and physical match activities in rugby league. Twenty-two professional rugby league players (age: 24.7 ± 4.0 years; height: 184.3 ± 4.7 cm; and body mass: 101.1 ± 9.9 kg) participated in the study. Training loads and physical match activities were monitored using global positioning systems. Total distance (m), high-speed running distance (VT 1IFT ; distance covered above estimated first ventilatory threshold [≥68% of 30-15 intermittent fitness test]) and total session rating of perceived exertion from all field-based (32.5 ± 8.5) and gym-based sessions (39.1 ± 12.5) were used to quantify preseason loads. Physical match activities were measured as total and high-speed running distance relative to individual playing time, whereas technical match activities and performance scores were used to evaluate individual match performance. To examine the relationship between physical match activities, technical performance, and preseason load, Pearson's correlation coefficients were quantified for each in-season game before performing a Fisher Z Transformation. Preseason high-speed running distance was positively associated with high-speed match activities ( r = 0.34-0.51), whereas negatively associated with technical performance scores ( r = -0.51 to -0.35) and hit up m·min -1 of match-play ( r = -0.30 to -0.17). It seems high speed running performed in the preseason period positively influences in-season physical match activity profiles, however, are unlikely to positively impact technical match performance.

The Relationship Between Match Tackle Outcomes and Muscular Strength and Power in Professional Rugby League.

ABSTRACT: Redman, KJ, Wade, L, Whitley, R, Connick, MJ, Kelly, VG, and Beckman, EM. The relationship between match tackle outcomes and muscular strength and power in professional rugby league. J Strength Cond Res 36(10): 2853-2861, 2022-Tackling is a fundamental skill in collision sports, such as rugby league. Match success is largely dependent on a player's ability to complete tackles and tolerate physical collisions. High levels of strength and power are key physical qualities necessary for effective tackling because players are required to generate large forces while pushing and pulling their opponents. The aim of this study was to examine the relationship between tackle outcomes and strength and power qualities in professional rugby league. Fourteen rugby league players participated in this study. Maximal strength was assessed through 1 repetition maximum on the back squat, bench press, and bench pull. Lower-body vertical and horizontal powers were evaluated using a countermovement jump and standing broad jump (SBJ), respectively. Upper-body power was assessed on a plyometric push-up (PPU). Postmatch analysis of 5 National Rugby League matches was conducted to examine tackling outcomes. A series of Spearman's rank-order correlations were used to assess the relationship among match tackle outcomes and strength and power variables. Significant associations were observed between play-the-ball speed and SBJ peak power (rs = -0.74, p = 0.003), postcontact metres and PPU peak power (rs = 0.77, p = 0.002), losing the play-the-ball contest in defence with SBJ distance (rs = 0.70, p = 0.006), and ineffective tackles with PPU concentric impulse (rs = 0.70, p = 0.007). These results suggest the development and maintenance of full-body power to enhance the likelihood of positive tackle outcomes during professional rugby league match-play.

Heterozygous disruption of ALAS1 in mice causes an accelerated age-dependent reduction in free heme, but not total heme, in skeletal muscle and liver.

5-Aminolevulinic acid (ALA) is the rate-limiting intermediate in heme biosynthesis in vertebrate species; a reaction catalyzed by the mitochondrial ALA synthase 1 (ALAS1) enzyme. Previously we reported that knockdown of the ubiquitously expressed ALAS1 gene in mice disrupts normal glucose metabolism, attenuates mitochondrial function and results in a prediabetic like phenotype when animals pass 20-weeks of age (Saitoh et al., 2018). Contrary to our expectations, the cytosolic and mitochondrial heme content of ALAS1 heterozygous (A1+/-) mice were similar to WT animals. Therefore, we speculated that regulatory "free heme" may be reduced in an age dependent manner in A1+/- mice, but not total heme. Here, we examine free and total heme from the skeletal muscle and liver of WT and A1+/- mice using a modified acetone extraction method and examine the effects of aging on free heme by comparing the amounts at 8-12 weeks and 30-36 weeks of age, in addition to the mRNA abundance of ALAS1. We found an age-dependent reduction in free heme in the skeletal muscle and liver of A1+/- mice, while WT mice showed only a slight decrease in the liver. Total heme levels showed no significant difference between young and aged WT and A1+/- mice. ALAS1 mRNA levels showed an age-dependent reduction similar to that of free heme levels, indicating that ALAS1 mRNA expression levels are a major determinant for free heme levels. The free heme pools in skeletal muscle tissue were almost 2-fold larger than that of liver tissue, suggesting that the heme pool varies across different tissue types. The expression of heme oxygenase 1 (HO-1) mRNA, which is expressed proportionally to the amount of free heme, were similar to those of free heme levels. Taken together, this study demonstrates that the free heme pool differs across tissues, and that an age-dependent reduction in free heme levels is accelerated in mice heterozygous for ALAS1, which could account for the prediabetic phenotype and mitochondrial abnormality observed in these animals.

Queuine links translational control in eukaryotes to a micronutrient from bacteria.

In eukaryotes, the wobble position of tRNA with a GUN anticodon is modified to the 7-deaza-guanosine derivative queuosine (Q34), but the original source of Q is bacterial, since Q is synthesized by eubacteria and salvaged by eukaryotes for incorporation into tRNA. Q34 modification stimulates Dnmt2/Pmt1-dependent C38 methylation (m5C38) in the tRNAAsp anticodon loop in Schizosaccharomyces pombe. Here, we show by ribosome profiling in S. pombe that Q modification enhances the translational speed of the C-ending codons for aspartate (GAC) and histidine (CAC) and reduces that of U-ending codons for asparagine (AAU) and tyrosine (UAU), thus equilibrating the genome-wide translation of synonymous Q codons. Furthermore, Q prevents translation errors by suppressing second-position misreading of the glycine codon GGC, but not of wobble misreading. The absence of Q causes reduced translation of mRNAs involved in mitochondrial functions, and accordingly, lack of Q modification causes a mitochondrial defect in S. pombe. We also show that Q-dependent stimulation of Dnmt2 is conserved in mice. Our findings reveal a direct mechanism for the regulation of translational speed and fidelity in eukaryotes by a nutrient originating from bacteria.

Inside the Belly of a Beast: Individualizing Nutrition for Young, Professional Male Rugby League Players: A Review.

Professional rugby league (RL) football is a contact sport involving repeated collisions and high-intensity efforts; both training and competition involve high energy expenditure. The present review summarizes and critiques the available literature relating the physiological demands of RL to nutritional requirements and considers potential ergogenic supplements that could improve players' physical capacity, health, and recovery during the preparatory and competition phases of a season. Although there may not be enough data to provide RL-specific recommendations, the available data suggest that players may require approximately 6-8 g·kg-1·day-1 carbohydrate, 1.6-2.6 g·kg-1·day-1 protein, and 0.7-2.2 g·kg-1·day-1 fat, provided that the latter also falls within 20-35% of total energy intake. Competition nutrition should maximize glycogen availability by consuming 1-4 g/kg carbohydrate (∼80-320 g) plus 0.25 g/kg (∼20-30 g) protein, 1-4 hr preexercise for 80-120 kg players. Carbohydrate intakes of approximately 80-180 g (1.0-1.5 g/kg) plus 20-67 g protein (0.25-0.55 g/kg) 0-2 hr postexercise will optimize glycogen resynthesis and muscle protein synthesis. Supplements that potentially improve performance, recovery, and adaptation include low to moderate dosages of caffeine (3-6 mg/kg) and ∼300 mg polyphenols consumed ∼1 hr preexercise, creatine monohydrate "loading" (0.3 g·kg-1·day-1) and/or maintenance (3-5 g/day), and beta-alanine (65-80 mg·kg-1·day-1). Future research should quantify energy expenditures in young, professional male RL players before constructing recommendations.

Effects of a Rugby League Match Simulation on Decision-Making in Elite Junior Rugby League.

ABSTRACT: Redman, K, Steel, K, Kelly, V, and Siegler, J. Effects of a rugby league match simulation on decision-making in elite junior rugby league. J Strength Cond Res 35(7): 1972-1980, 2021-The paucity of research on decision-making in Rugby league within the context of a match, and the potential for age and playing experience to influence decision-making ability, warrants further investigation. Therefore, the aim of this study was to determine the effects of a simulated rugby league match on decision-making in elite junior rugby league players. Twenty male junior rugby league players (age 15.9 ± 0.9 years, body mass 87.3 ± 14.3 kg, height 1.82 ± 0.07 m, playing experience 10.2 ± 2.4 years) completed a match simulation protocol that replicated the physiological demands of an elite junior rugby league match. Decision-making performance was measured prematch, midmatch, and postmatch simulation through a reactive agility test and video-based temporal occlusion task. Upper- and lower-body neuromuscular measures as well as ratings of perceived exertion were obtained before decision-making assessments to quantify fatigue. A 2-way mixed analysis of variance with Bonferroni corrections was used to assess changes during the simulation, and significance was accepted at p < 0.05. The main finding of this study was despite observing a decline in neuromuscular function during the match simulation protocol (countermovement jump declined 1.9 ± 0.6 cm [95% confidence intervals 0.4-3.5]; p < 0.05), this decline did not significantly affect decision-making performance (reactive agility test [p = 0.58]; temporal occlusion [p = 0.88]). These results suggest elite junior rugby league players are capable of sustaining performance during a progressively fatiguing match simulation.

Lower-Body Aquatic Training Prescription for Athletes.

ABSTRACT: Holmberg, PM, Gorman, AD, Jenkins, DG, and Kelly, VG. Lower-body aquatic training prescription for athletes. J Strength Cond Res 35(3): 859-869, 2021-Traditionally prescribed to manage various medical ailments and promote healing, aquatic immersion may provide a favorable environment to undertake preparatory training tasks given its profound biological effects that extend across most homeostatic systems. In addition to understanding these effects, which are related to the fundamental principles of hydrodynamics, there is a need to consider the acute physiomechanical responses to alterations of key constraints associated with particular preparatory tasks. Evidence suggests that the manipulation of different constraints during aquatic training can enable a unique setting to complement, supplement, or supplant land-based programming to maintain or improve physical capacities in athletes without exacerbating physiological stress. Thus, this article focuses on the interplay of constraints and their associated outcomes to provide information that can be used to program lower-body aquatic training for athletes. In addition, the aim of the article is to summarize the literature on aquatic training to highlight the outcomes that occur when certain constraints are manipulated.

Relationship Between Tennis Serve Velocity and Select Performance Measures.

ABSTRACT: Hayes, MJ, Spits, DR, Watts, DG, and Kelly, VG. Relationship between tennis serve velocity and select performance measures. J Strength Cond Res 35(1): 190-197, 2021-The purpose of this study was to determine whether there was a relationship between tennis serve speed and isometric midthigh pull (IMTP) kinetic variables: countermovement jump (CMJ) height, shoulder internal and external rotation strength, and anthropometric measures in elite adolescent tennis players. Twenty-one elite junior tennis players from the Tennis Australia National Academy were recruited for this study (male, n = 12 and female, n = 9). Correlations between the performance variables and peak tennis serve speed were calculated using a Pearson's product-moment correlation coefficient. A significant positive correlation was found between peak serve speed and body height (r = 0.80, p < 0.01), IMTP peak force (r = 0.87, p < 0.01), CMJ height (r = 0.77, p ≤ 0.01), and impulse at 300 ms (r = 0.71, p ≤ 0.01). A significant, strong correlation was found between peak serve speed and impulse at 100 ms (r = 0.58, p ≤ 0.01), impulse at 200 ms (r = 0.64, p ≤ 0.01), internal rotation <90° (r = 0.63, p ≤ 0.01), and external rotation <90° (r = 0.63, p ≤ 0.01). Because of the significant positive correlations between IMTP variables, CMJ height and peak serve speed, strength and conditioning coaches with access to a force plate should consider using the IMTP to athletically profile athletes in regards to their strength, power, and injury risk.

Monitoring Prescribed and Actual Resistance Training Loads in Professional Rugby League.

ABSTRACT: Redman, KJ, Connick, MJ, Beckman, EM, and Kelly, VG. Monitoring prescribed and actual resistance training loads in professional rugby league. J Strength Cond Res 35(6): 1604-1610, 2021-Coaches devote a considerable amount of time and effort prescribing and selecting exercises to elicit training adaptations. Adherence to the prescribed resistance training load may vary for a number of reasons. The aim of this study was to quantify the difference between prescribed and actual resistance training loads in a team of professional rugby league players. Training loads were quantified using volume load and training intensity throughout a season. The competition was categorized into preseason, early competition, mid-competition, and late competition. Twenty-seven players participated in this study. Four exercises were monitored: back squat, bench press, bench pull, and clean pull. A Friedman's test was used to assess differences between prescribed and actual training loads throughout different phases of the season, for different exercises, and during different weeks in a training block. There were significantly greater differences in prescribed and actual volume loads during the mid-competition in comparison to all other phases of the season (p < 0.01). Although players adherence to prescribed training intensity was significantly greater during the preseason compared with the remainder of the season (p < 0.05), they completed significantly less prescribed training load during week 1 in comparison to week 4 within a training block (p < 0.05). The results of this study demonstrate that regular monitoring of completed resistance training loads may be of greater importance to strength and conditioning coaches to assist in examining potential progress and fatigue or allow for more accurate prescription of load to enhance adaptation throughout a season.

Seasonal Changes in Strength and Power in Elite Rugby League: A Systematic Review and Meta-Analysis.

The ability of a rugby league player to express high levels of strength and power is crucial for success in competition. Although researchers have shown relationships between physical attributes and performance, there is a lack of information related to changes in strength and power across various phases of the season. The purpose of this review was to identify the magnitude of change in muscular strength and power during different phases of an elite rugby league season. Percentage change and effect size were calculated to evaluate the magnitude of changes in strength and power. Meta-analyses were conducted to provide pooled estimates and 95% confidence intervals. Twelve studies were included; six reported changes following pre-season training, two during the competition phase and four examined changes over multiple seasons. Pooled estimates indicated large increases in upper and lower body strength 0.81 [0.43-1.19] and 1.35 [0.79-1.91], respectively, following pre-season training. Studies incorporating predominantly heavier loads (p 80% 1 RM) in training reported greater increases in maximal strength than studies completing lighter loads. Four studies used a wave-like programming strategy to obtain large improvements in strength and power 0.91 [0.36-1.45] and 0.90 [0.22-1.57], over multiple seasons. The results of this review highlight the limited current evidence and provides a preliminary reference point for strength and conditioning coaches aiming to develop and maintain strength and power across various stages, and over multiple seasons. Importantly, the results also indicate that higher loads result in greater increases in strength than lower loads.

Highly efficient CRISPR-targeting of the murine Hipp11 intergenic region supports inducible human transgene expression.

Safe harbor loci allow predicable integration of a transgene into the genome without perturbing endogenous gene activity and for decades have been exploited in the mouse to investigate gene function, generate humanised models and create tissue specific reporter and Cre recombinase expressing lines. Herein, we show that the murine Hipp11 intergenic region can facilitate highly efficient integration of a large transgene-the human CD1A promoter and coding region-by means of CRISPR-Cas9 mediated homology directed repair. The data shows that the single copy human CD1A transgene is faithfully expressed in an inducible manner in homozygous animals in both macrophage and dendritic cells. Our results validate the Hipp11 intergenic region as being a highly amenable target site for functional transgene integration in mouse.