RESUMO
The expression of Sgs genes in the salivary gland of the third instar larva of Drosophila is a spatially restricted response to signalling by the steroid hormone 20-hydroxyecdysone. For Sgs-4, we have previously demonstrated that its strictly tissue and stage-specific expression is the result of combined action of the ecdysone receptor and secretion enhancer binding proteins (SEBPs). One of these SEBPs, SEBP2, was shown to be the product of the homeotic gene fork head. Together with SEBP3, SEBP2 appears to be responsible for the spatial restriction of the hormone response of Sgs-4. Here, we show that SEBP3 is a heterogeneous binding activity that consists of different helix-loop-helix (HLH) proteins. We cloned the Drosophila homologue of human transcription factor AP-4 (dAP-4) and identified it as one of these HLH proteins. The dAP-4 protein shows great similarity to its human and Caenorhabditis counterparts within the bHLHZip domain, the second leucine zipper dimerization motif, and a third region of unknown function. The expression pattern of dAP-4 indicates that it is a ubiquitously expressed HLH protein in Drosophila. As a second component of SEBP3 we identified the Daughterless (Da) protein, which is also ubiquitously expressed and binds to SEBP3 sites independent of dAP-4. Since both dAP-4 and Da can be detected in situ at transposed Sgs-4 transcriptional control elements in polytene salivary gland chromosomes, we propose that each of the two proteins contributes to the transcriptional control of Sgs-4.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/genética , Regulação da Expressão Gênica , Proteínas do Grude Salivar de Drosophila/genética , Proteínas de Homeodomínio/metabolismo , Proteínas Nucleares/metabolismo , Fatores de Transcrição/metabolismo , Sequência de Aminoácidos , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos , Sítios de Ligação , Elementos de DNA Transponíveis/genética , Proteínas de Ligação a DNA/química , Drosophila melanogaster/metabolismo , Fatores de Transcrição Forkhead , Sequências Hélice-Alça-Hélice , Humanos , Proteínas de Insetos/metabolismo , Proteínas dos Microfilamentos , Dados de Sequência Molecular , Proteínas Nucleares/química , Conformação Proteica , Sequências Reguladoras de Ácido Nucleico , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/química , Ativação TranscricionalRESUMO
The Drosophila melanogaster gene Sgs-1 belongs to the secretion protein genes, which are coordinately expressed in salivary glands of third instar larvae. Earlier analysis had implied that Sgs-1 is located at the 25B2-3 puff. We cloned Sgs-1 from a YAC covering 25B2-3. Despite using a variety of vectors and Escherichia coli strains, subcloning from the YAC led to deletions within the Sgs-1 coding region. Analysis of clonable and unclonable sequences revealed that Sgs-1 mainly consists of 48-bp tandem repeats encoding a threonine-rich protein. The Sgs-1 inserts from single lambda clones are heterogeneous in length, indicating that repeats are eliminated. By analyzing the expression of Sgs-1/lacZ fusions in transgenic flies, cis-regulatory elements of Sgs-1 were mapped to lie within 1 kb upstream of the transcriptional start site. Band shift assays revealed binding sites for the transcription factor fork head (FKH) and the factor secretion enhancer binding protein 3 (SEBP3) at positions that are functionally relevant. FKH and SEBP3 have been shown previously to be involved in the regulation of Sgs-3 and Sgs-4. Comparison of the levels of steady state RNA and of the transcription rates for Sgs-1 and Sgs-1/lacZ reporter genes indicates that Sgs-1 RNA is 100-fold more stable than Sgs-1/lacZ RNA. This has implications for the model of how Sgs transcripts accumulate in late third instar larvae.
Assuntos
Drosophila melanogaster/genética , Regulação da Expressão Gênica , Proteínas do Grude Salivar de Drosophila/genética , Sequência de Aminoácidos , Animais , Cromossomos Artificiais de Levedura , Clonagem Molecular , Elementos Facilitadores Genéticos , Proteínas do Grude Salivar de Drosophila/química , Larva , Dados de Sequência Molecular , Sequências Repetitivas de Ácido Nucleico , Mapeamento por Restrição , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Glândulas Salivares/fisiologia , Alinhamento de Sequência , Deleção de Sequência , Homologia de Sequência do Ácido Nucleico , TreoninaRESUMO
P transposon induced modifier mutations of position-effect variegation (PEV) were isolated with the help of hybrid dysgenic crosses (pi 2 strain) and after transposition of the mutator elements pUChsneory+ and P[lArB]. Enhancer mutations were found with a ten times higher frequency than suppressors. The 19 pUChsneory(+)- and 15 P[lArB]-induced enhancer mutations can be used for cloning of genomic sequences at the insertion sites of the mutator elements via plasmid rescue. Together with a large sample of X-ray-induced (48) and spontaneous (93) enhancer mutations a basic genetic analysis of this group of modifier genes was performed. On the basis of complementation and mapping data we estimate the number of enhancer genes at about 30 in the third chromosome and between 50 and 60 for the whole autosome complement. Therefore, enhancer of PEV loci are found in the Drosophila genome as frequently as suppressor genes. Many of the enhancer mutations display paternal effects consistent with the hypothesis that some of these mutations can induce genomic imprinting. First studies on the developmentally regulated gene expression of PEV enhancer genes were performed by beta-galactosidase staining in P[lArB] induced mutations.
Assuntos
Elementos de DNA Transponíveis/genética , Drosophila melanogaster/genética , Elementos Facilitadores Genéticos , Genes Supressores , Animais , Cruzamentos Genéticos , Elementos Facilitadores Genéticos/efeitos da radiação , Feminino , Teste de Complementação Genética , Hibridização Genética/genética , Masculino , Mutagênese , Ovário/química , Monoéster Fosfórico Hidrolases/genética , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Testículo/química , Dedos de Zinco/genéticaRESUMO
We identified two regulatory elements in the upstream region of the Drosophila Sgs-3 gene which are both able to bind the ecdysone receptor (EcR/USP) and the product of the fork head gene. Interestingly, only one of the EcR/USP binding sites is able to recognize in vitro-translated EcR/USP, which provides evidence for the existence of different receptor forms having different DNA binding specificities. Deletions of the elements lead to a reduced accumulation of Sgs-3 mRNA without altering the temporal expression profile of the gene. The data are consistent with the hypothesis that the ecdysone receptor directly contributes to the transcriptional activation of Sgs-3 by binding to at least one of the two elements. Since also the Sgs-4 gene is controlled by a functional EcR/USP binding site, a direct participation of EcR/USP in the formation of regulatory complexes may be of general importance for the hormonal control of Sgs genes.
Assuntos
Drosophila/genética , Proteínas do Grude Salivar de Drosophila/genética , Proteínas Nucleares/metabolismo , Receptores de Esteroides/metabolismo , Fatores de Transcrição/metabolismo , Animais , Sítios de Ligação , Ecdisona/metabolismo , Fatores de Transcrição Forkhead , Hormônios de Inseto/metabolismo , Transcrição GênicaRESUMO
Domina (Dom) is a novel member of the FKH/WH transcription factor gene family of Drosophila. Two alternatively polyadenylated Dom transcripts of 2.9 and 3.9 kb encode a 719-amino-acid protein with a FKH/WH domain and a putative acidic transactivation domain. Dom is mainly expressed in the central and peripheral nervous system. Homozygous mutants show rough eyes, irregular arrangement of bristles, extended wings, defective posterior wing margins, and a severely diminished vitality and fertility. Heterozygous Dom flies are morphologically wild type but show suppression of position-effect variegation. Consistently with this chromatin effect DOM protein is accumulated in the chromocenter and, as expected from a transcription factor, is found at specific euchromatic loci. Sequence comparison suggests that DOM of Drosophila is homologous to the chordate WHN proteins. The chromatin modifying capability of DOM is probably based on the FKH/WH domain, which shows a remarkable structural similarity to the winged-helix structures of H1 and the central globular domain of H5.
Assuntos
Drosophila/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Nucleares/genética , Fatores de Transcrição/genética , Alelos , Sequência de Aminoácidos , Animais , Núcleo Celular/metabolismo , Cromatina/química , Cromatina/metabolismo , Cromossomos/ultraestrutura , DNA Complementar/metabolismo , Drosophila/embriologia , Feminino , Fatores de Transcrição Forkhead , Heterozigoto , Homozigoto , Imuno-Histoquímica , Hibridização In Situ , Masculino , Modelos Genéticos , Dados de Sequência Molecular , Mutagênese , Mutação , Proteínas Nucleares/química , Fenótipo , Células Fotorreceptoras de Invertebrados/embriologia , Pigmentação/genética , Poli A/metabolismo , Estrutura Terciária de Proteína , RNA/metabolismo , RNA Mensageiro/metabolismo , Glândulas Salivares/metabolismo , Homologia de Sequência de Aminoácidos , Fatores Sexuais , Fatores de Tempo , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Ativação Transcricional , Asas de Animais/embriologiaRESUMO
The ring gland function of Drosophila melanogaster is controlled by the CNS. To identify genes that are active in brain cells and are involved in the ring gland control, we analysed enhancer trap lines with respect to CNS- and/or ring gland-specific lacZ expression in third-instar larvae. From one of the enhancer trap lines, which shows specific lacZ expression in the CNS and prothoracic part of the ring gland, the mub gene was cloned. The gene is strongly expressed in the mushroom bodies throughout development. Nucleotide sequence analysis of cDNA clones revealed a high degree of similarity to vertebrate RNA binding KH domain proteins, suggesting a function of the MUB protein in binding and stabilizing of specific mRNAs in the mushroom bodies. Null mutants of the mub gene do not exhibit a visible mutant phenotype. We speculate, therefore, that the mub gene is involved in learning and memory processes.
Assuntos
Proteínas de Ligação a DNA/genética , Drosophila melanogaster/genética , Gânglios dos Invertebrados/metabolismo , Genes de Insetos/genética , Proteínas de Insetos/genética , Proteínas de Ligação a RNA/genética , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação/genética , Encéfalo/citologia , Encéfalo/embriologia , Encéfalo/metabolismo , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , Drosophila melanogaster/química , Drosophila melanogaster/embriologia , Elementos Facilitadores Genéticos , Feminino , Gânglios dos Invertebrados/química , Gânglios dos Invertebrados/embriologia , Expressão Gênica/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Insetos/química , Masculino , Dados de Sequência Molecular , Mutação/genética , Estrutura Terciária de Proteína , RNA/análise , RNA/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Transformação Genética/genética , beta-Galactosidase/análise , beta-Galactosidase/genéticaRESUMO
In insects, peptidergic neurons of the central nervous system regulate the synthesis of the main developmental hormones. Neuropeptides involved in this neuroendocrine cascade have been identified in lepidopterans and dictyopterans. Since these organisms are not suitable for genetic research, we identified peptidergic brain neurons innervating the ring gland in Drosophila melanogaster. In larvae of Drosophila, ecdysteroids and juvenile hormones are produced by the ring gland, which is composed of the prothoracic gland, the corpus allatum, and the corpora cardiaca. Using the GAL4 enhancer trap system, we mapped those neurons of the central nervous system that innervate the ring gland. Eleven groups of neurosecretory neurons and their target tissues were identified. Five neurons of the lateral protocerebrum directly innervate the prothoracic gland or corpus allatum cells of the ring gland and are believed to regulate ecdysteroid and juvenile hormone titers. Axons of the circadian pacemaker neurons project onto dendritic fields of these five neurons. This connection might be the neuronal substrate of the circadian rhythms of molting and metamorphosis in Drosophila. Most of the neurons presented here have not been described before. The enhancer trap lines labeling them will be valuable tools for the analysis of neuronal as well as genetic regulation in insect development.
Assuntos
Drosophila melanogaster/fisiologia , Fenômenos Fisiológicos do Sistema Nervoso , Neurônios/fisiologia , Proteínas de Saccharomyces cerevisiae , Animais , Animais Geneticamente Modificados , Ritmo Circadiano/fisiologia , Proteínas de Ligação a DNA , Elementos Facilitadores Genéticos , Proteínas Fúngicas/genética , Genótipo , Proteínas de Fluorescência Verde , Hormônios de Inseto/fisiologia , Insetos , Larva , Lepidópteros , Proteínas Luminescentes/análise , Sistema Nervoso/citologia , Neurônios/citologia , Neuropeptídeos/fisiologia , Sistemas Neurossecretores/citologia , Sistemas Neurossecretores/fisiologia , Fatores de Transcrição/genéticaRESUMO
The number of genomic DNA or cDNA sequences of Schistosoma mekongi accessible in Genbank or EMBL is very limited up to now. Recently, two reports have appeared on the molecular phylogeny of Schistosoma species inferred from partial sequence data of rRNA genes; no further sequence data of S. mekongi is available yet. Knowledge of the molecular structure of protein coding genes of S. mekongi will provide a better understanding of gene function in the genus Schistosoma. A cDNA library of S. mekongi adult male was constructed and a cDNA encoding the 26 kDa glutathione S-transferase protein of this species was cloned. Sequence analysis of this cDNA confirmed the close phylogenetic relationship of S. mekongi to S. japonicum.
Assuntos
DNA Complementar/genética , DNA de Helmintos/genética , Glutationa Transferase/genética , Schistosoma japonicum/enzimologia , Schistosoma japonicum/genética , Análise de Sequência de DNA , Sequência de Aminoácidos , Animais , Sequência de Bases , Biblioteca Gênica , Masculino , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Schistosoma japonicum/classificação , Alinhamento de SequênciaRESUMO
A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28.7 kDa. It shows sequence identity values between 57.6 and 58.1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period.
Assuntos
Proteínas 14-3-3/genética , Proteínas 14-3-3/imunologia , Fasciola/genética , Fasciolíase/imunologia , Proteínas 14-3-3/química , Sequência de Aminoácidos , Animais , Anticorpos Anti-Helmínticos/sangue , Anticorpos Anti-Helmínticos/imunologia , Antígenos de Helmintos/química , Antígenos de Helmintos/genética , Antígenos de Helmintos/imunologia , Clonagem Molecular , Fasciola/crescimento & desenvolvimento , Fasciola/imunologia , Fasciola/metabolismo , Feminino , Biblioteca Gênica , Masculino , Camundongos , Dados de Sequência Molecular , Especificidade de Órgãos , RNA de Helmintos/genética , RNA de Helmintos/isolamento & purificação , RNA de Helmintos/metabolismo , Coelhos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Análise de Sequência de DNARESUMO
The structural gene Sgs-4 responsible for larval saliva protein 4 of Drosophila melanogaster was localized, with the help of Notch deficiencies, within the section between bands 3C10 and 3D1 of the X chromosome. In this chromosome section there is, very probably, only one fine band. In the third larval instar chromosome this section is transcriptionally active and forms a puff. When the ecdysone concentration increases, about 5 h before prepupa formation, it becomes inactive.--In section 3C of X chromosomes of third instar larvae of the stock Hikone-R no puff is formed. The saliva of these larvae lacks protein 4. However, female hybrids (H/B and H/O) from Hikone-R crossed with Berlin and Oregon respectively produce a Hikone-specific saliva protein 4h. The synthesis of protein 4h in the hybrids H/B and H/O is ascribed to an activation of the gene Sgs-4 in the Hikone chromosome.--In the saliva of heterozygotes (FM1/H) carrying one inversion chromosome In(1)FM1 and one X chromosome from Hikone, protein 4h could not be detected. In these inversion heterozygotes in 90% of all nuclei the homologues are not paired in 3C, and 3C is puffed only in the FM1 chromosome. This suggests that a precondition for the activation of Hikone gene Sgs-4 in heterozygotes may be intimate homologue pairing.--Intersexes with one of their X chromosomes from Hikone-R and the other from Berlin produce relatively more protein 4h than do diploid H/B females, indicating facilitated transcription as a result of dosage compensation.
Assuntos
Cromossomos , Drosophila melanogaster/metabolismo , Glândulas Salivares/metabolismo , Proteínas e Peptídeos Salivares/biossíntese , Animais , Ecdisona , Feminino , Genes , Hibridização Genética , Larva , Cromossomos SexuaisRESUMO
Secretion proteins from larval salivary glands of Drosophila melanogaster were analyzed with acrylamide gel electrophoresis. Four fractions were found; three showed electrophoretic variants in different wild-type stocks. Crossbreeding and cytogenetic techniques were used to localize the genes responsible for the two main fractions: The gene for fraction 3 was found to lie within a segment of the third chromosome which includes section 68C; the gene for fraction 4, Sgs-4, was found to lie within section 3C8-3D1 of the X chromosome (1 - 3-5). The puffs within these sections of the giant chromosomes are active before and during secretion synthesis and become inactive as secretion synthesis ceases. Larvae of one wild-type stock which lack protein fraction 4 do not exhibit any puffing in 3C. The relative amount of protein 4 in the salivary secretion shows a marked dependence on the dosage of the Sgs-4 gene in both duplication and deficiency genotypes. The active site within puff 3C11-12 apparently contains the structural gene for protein 4.
Assuntos
Cromossomos/metabolismo , Drosophila melanogaster/metabolismo , Biossíntese de Proteínas , Mapeamento Cromossômico , Genes , Larva , Proteínas/metabolismo , Recombinação Genética , Saliva , Glândulas Salivares/metabolismo , Glândulas Salivares/ultraestrutura , Cromossomos Sexuais , Fatores SexuaisRESUMO
Larval salivary gland secretion from seven wild-type stocks of Drosophila melanogaster was electrophoretically analyzed. Considerable variability occurs in the X-chromosomally coded secretion protein 4, both qualitatively, as expressed by differences in electrophoretic mobilities, and quantitatively as seen by its relative amount in the secretion. Drosophila stocks with "normal" amounts of protein 4 show approximately 80-90% dosage compensation in the males, whereas in two stocks with lower amounts of protein 4 there is no indication of dosage compensation. Genetic analysis showed that the properties of secretion protein 4 and the level of expression of the Sgs-4 gene are controlled by the X-chromosome. Recombination experiments indicate that the stock-specific characteristics of protein 4 are properties of the structural gene Sgs-4 itself or of a chromosome region immediately adjacent to Sgs-4. One recombinant (R+79), manifesting an intermediate level of dosage compensation, indicates that a chromosome segment closely distal to Sg-4 is responsible for the regulation of the gene and for dosage compensation in particular. Accordingly, Sgs-4 must be transcribed from distal to proximal. Its position on the genetic map is 3.6. Two stocks, Hikone-R and Kochi-R, which were originally described as 0-mutants produce very low amounts of a specific secretion protein, 4 h, as revealed by a transvection effect and also by fluorography of overloaded gels.
Assuntos
Drosophila melanogaster/genética , Genes , Variação Genética , Proteínas e Peptídeos Salivares/genética , Animais , Mapeamento Cromossômico , Drosophila melanogaster/fisiologia , Eletroforese em Gel de Poliacrilamida , Feminino , Larva/genética , MasculinoRESUMO
The X chromosomal gene Sgs-4 coding for a larval secretion protein of Drosophila melanogaster is expressed stage and tissue specifically and is hyperexpressed in male larvae of most Drosophila stocks which show dosage compensation. We analysed three Sgs-4 alleles which differ in the size of their coding region, in the intensity of their expression and in the level of dosage compensation. The size and amount of Sgs-4 proteins directly reflect those of RNAs. Different RNA sizes result from different numbers of 21 bp repeats within the structural genes. We sequenced 2.8 kb of DNA upstream of the transcription initiation site of the three alleles. Sequences known to be essential for correct gene expression were located. The only difference within DNA sequences from -1 to -1200 between two alleles with different degrees of expression and differing in dosage compensation is a C to T transition at -344 within a supposed consensus sequence for ecdysone receptor complex binding (ECR). This mutation is partly located within a region of dyad symmetry. Alleles with identical expression show identical mutations within a GTT-rich region at -1.2 kb, but differ within a GT-rich region at -2.0 kb. A polyadenylated 0.5 kb RNA was found to be transcribed from the GTT-rich region of the strand opposite to that of Sgs-4. The corresponding gene is active only in larval salivary glands and, therefore, is named gland specific gene, gsg.
Assuntos
Drosophila melanogaster/genética , Genes , Proteínas do Grude Salivar de Drosophila/genética , Proteínas e Peptídeos Salivares/genética , Alelos , Animais , Sequência de Bases , DNA/genética , Mecanismo Genético de Compensação de Dose , Feminino , Genes Reguladores , Masculino , Dados de Sequência Molecular , Cromossomo XRESUMO
Here we describe the identification of four binding sites of secretion enhancer binding protein 2 (SEBP2) in the regulatory region of the Drosophila salivary gland secretion protein gene 4 (Sgs-4) and show that despite these sites' correspondence with previously described Broad-Complex protein binding sites, SEBP2 is a Broad-Complex-independent factor encoded by the region-specific homeotic gene fork head (fkh). Two of the Fork head/SEBP2 binding sites are located within an ecdysone response unit which controls the tissue- and stage-specific responses of Sgs-4 to the steroid hormone 20-hydroxyecdysone. We demonstrate that these binding sites are relevant to the transcriptional activation of Sgs-4 and show that Fork head also binds to the Sgs-4 ecdysone response unit in vivo. Aside from being involved in the control of decisions during embryonic development, fkh thus participates directly in the control of specialized functions of differentiated cells at later stages of development.
Assuntos
Proteínas do Grude Salivar de Drosophila/genética , Hormônios de Inseto/genética , Proteínas Nucleares/genética , Fatores de Transcrição/genética , Animais , Sítios de Ligação , DNA/química , Drosophila , Ecdisona/metabolismo , Ecdisterona/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Fatores de Transcrição Forkhead , Proteínas do Grude Salivar de Drosophila/metabolismo , Hormônios de Inseto/metabolismo , Proteínas Nucleares/metabolismo , Conformação Proteica , Receptores de Esteroides/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Fatores de Transcrição/metabolismo , Transcrição GênicaRESUMO
The steroid hormone 20-hydroxyecdysone controls both induction and repression of the Drosophila 'intermolt gene' Sgs-4. We show here that the ecdysone receptor binds to two sites, element I and element II, in the regulatory region of Sgs-4. A functional analysis revealed that element II appears to be of no importance for Sgs-4 expression, while element I proved to be an ecdysone response element that is necessary, but not sufficient, for induction of Sgs-4 expression. Our results provide no evidence that repression of Sgs-4 expression is mediated by one of the two receptor binding sites. In the close vicinity of elements I and II, we detected two binding sites of secretion enhancer binding protein 3 (SEBP 3). Like receptor element I, one of these sites also proved to be necessary, but not sufficient, for expression of Sgs-4. Therefore, induction of Sgs-4 requires binding of both ecdysone receptor and SEBP 3 to a complex hormone response unit, which also contains binding sites for a third factor, SEBP 2. The SEBP 2 sites coincide with binding sites of products of the Broad-Complex locus, which has been implicated recently with transduction of the hormonal signal. Thus, the available data suggest that induction of Sgs-4, and possibly other 'intermolt genes', is a combination of a primary and a secondary response to the hormone.
Assuntos
Proteínas de Ligação a DNA/fisiologia , Drosophila melanogaster/genética , Ecdisona/farmacologia , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Proteínas do Grude Salivar de Drosophila/fisiologia , Proteínas de Choque Térmico/genética , Receptores de Esteroides/fisiologia , Fatores de Transcrição/fisiologia , Animais , Sequência de Bases , Genes de Insetos , Larva , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Sondas de Oligonucleotídeos , RNA Mensageiro/genética , Sequências Reguladoras de Ácido Nucleico , Glândulas Salivares , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Relação Estrutura-AtividadeRESUMO
The Drosophila nuclear proteins Bj6 and Bx42 characterized previously are detected in a series of developmentally active puffs on salivary gland chromosomes. Here the binding of both proteins at puff 3C11-12 containing the glue protein gene Sgs-4 is described in more detail. By deletion analysis we show that both proteins bind within a chromosomal segment containing 17-19 kb of DNA surrounding the Sgs-4 gene. They are detectable at this site during the intermoult stages, before the puff regresses in response to the moulting hormone ecdysone. If the Sgs-4 gene together with flanking DNA sequences is brought into a different chromosomal position by P element transfer, both proteins are detected at this new location. Both proteins are bound to the chromosome within the range of 2.5 kb DNA upstream of the Sgs-4 gene. A strain containing a 52 bp deletion within this region fails to bind Bx42 protein suggesting that the missing DNA, which overlaps a hypersensitive region, may be required for the binding of the Bx42 protein.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Drosophila melanogaster/genética , Genes , Proteínas do Grude Salivar de Drosophila/genética , Hormônios de Inseto/metabolismo , Proteínas Nucleares/metabolismo , Proteínas e Peptídeos Salivares/genética , Animais , Bandeamento Cromossômico , Cromossomos/metabolismo , DNA/metabolismo , Elementos de DNA Transponíveis , Fatores de Tempo , Transformação GenéticaRESUMO
Sgs-4 is one of the eight known genes coding for larval secretion proteins in Drosophila melanogaster. High-level transcription of the endogenous Sgs genes in salivary glands is accompanied by chromosome puffing at the Sgs gene loci. Naturally occurring mutations of the Sgs-4 promoter region diminish both the level of Sgs-4 expression and the puff size; in null-producers no puff is formed. P element-mediated transformation experiments were performed to clarify this apparent causal relation between transcription and puffing. Sgs-4 upstream sequences, unchanged or recombined with sequences from differently expressed alleles, were fused with Sgs-4 coding and downstream sequences or with the coding sequence of the viral oncogene v-mil. Analyses of the expression of these fragments at the RNA and protein levels and of their capacity for puff formation demonstrate uncoupling of transcription and puffing. That is, high-level transcription is independent of chromosome puffing and does not necessarily induce puffing, and developmentally regulated chromosome puffing is independent of significant transcriptional activity within the puff. Our results show that the strength of the Sgs-4 promoter located within the upstream region from -1 to -840 determines the formation of a puff. No specific effects could be detected on either transcription or puffing by decondensed versus compact chromatin adjoining the transposed DNA at the sites of insertion in transformants. A model in which trans-acting factors binding to the promoter region initiate puffing is proposed.
Assuntos
Mapeamento Cromossômico , Drosophila melanogaster/genética , Expressão Gênica , Regiões Promotoras Genéticas , Proteínas e Peptídeos Salivares/genética , Cromossomo X , Animais , Elementos de DNA Transponíveis , Drosophila melanogaster/crescimento & desenvolvimento , Feminino , Larva , Transcrição GênicaRESUMO
The Sgs-4 gene of Drosophila melanogaster encodes one of the larval secretion proteins and is active only in salivary glands at the end of larval development. This gene lies in the X chromosome and is controlled by dosage compensation--i.e., the gene is hyperexpressed in males. Therefore, males with one X chromosome produce nearly as much Sgs-4 products as females with two X chromosomes. We used a 4.9-kilobase-pair (kb) DNA fragment containing the Sgs-4d coding region embedded in 2.6 kb of upstream sequences and 1.3 kb of downstream sequences for P-element-mediated transformation of the Sgs-4h underproducer strain Kochi-R. Sgs-4d gene expression was found in all 15 transformed lines analyzed, varying with the site of chromosomal integration. The transposed gene was subject to tissue- and stage-specific regulation. At X-chromosomal sites, the levels of gene expression were similar in both sexes, signifying dosage compensation. At autosomal sites, it was on average 1.5 times higher in males than in females. The results indicate that the transforming DNA fragment contains all sequences necessary for tissue- and stage-specific regulation and for hyperexpression in males.
Assuntos
Drosophila melanogaster/genética , Proteínas e Peptídeos Salivares/genética , Fatores Etários , Animais , DNA Recombinante , Mecanismo Genético de Compensação de Dose , Feminino , Genes , Genes Reguladores , Masculino , RNA Mensageiro/genética , Distribuição Tecidual , Transformação Genética , Translocação GenéticaRESUMO
The X chromosomally located allele Sgs-4c for a larval secretion protein of Drosophila melanogaster is normally expressed in female larvae of the strain Oregon R and is hyperexpressed in male larvae exhibiting dosage compensation; the allele Sgs-4d in the strain Samarkand is weakly expressed and is not hyperexpressed in male larvae showing a dosage effect. P element-mediated transformation of upstream DNA sequences from both alleles combined with Sgs-4d coding and downstream sequences was performed to localize sequences which are responsible for the level of gene expression and for hyperexpression of Sgs-4c in male larvae. Our results demonstrate that weak expression and dosage effect are inherited with the upstream region from -1 to -838. This Samarkand fragment differs from the homologous Oregon R region only by a C to T transition at -344 which lies within an assumed binding sequence for the ecdysone receptor complex of dyad base symmetry. Replacing the Samarkand upstream region from -1 to -838 by the Oregon R region restores normal Sgs-4 expression and dosage compensation. Hyperexpression in male larvae displays high sensitivity to position effect and is nearly completely inhibited in one transformed line under heterozygous conditions. The integration of an Sgs-4d transposon into a weak spot of polytene chromosome 2L results in a decrease in gene expression. The GTT- and GT-rich regions at -1.2 and -2.0 kb do not obviously influence Sgs-4 expression but possibly play a role in induction of stage-specific chromosome puffing.
Assuntos
Drosophila melanogaster/genética , Genes Reguladores , Proteínas do Grude Salivar de Drosophila/genética , Proteínas e Peptídeos Salivares/genética , Animais , Elementos de DNA Transponíveis , Mecanismo Genético de Compensação de Dose , Feminino , Regulação da Expressão Gênica , Masculino , Plasmídeos , Transformação Genética , Cromossomo X/ultraestruturaRESUMO
Pipsqueak (Psq) belongs to a family of proteins defined by a phylogenetically old protein-protein interaction motif. Like the GAGA factor and other members of this family, Psq is an important developmental regulator in Drosophila, having pleiotropic functions during oogenesis, embryonic pattern formation, and adult development. The GAGA factor controls the transcriptional activation of homeotic genes and other genes by binding to control elements containing the GAGAG consensus motif. Binding is associated with formation of an open chromatin structure that makes the control regions accessible to transcriptional activators. We show here that Psq contains a novel DNA-binding domain, which binds, like the GAGA factor zinc finger DNA-binding domain, to target sites containing the GAGAG consensus motif. Binding is suppressed, as in the GAGA factor and other proteins of the family, by the associated protein-protein interaction motif. The DNA-binding domain, which we call the Psq domain, is identical with a previously identified region consisting of four tandem repeats of a conserved 50-amino acid sequence, the Psq motif. The Psq domain seems to be structurally related to known DNA-binding domains, both in its repetitive character and in the putative three-alpha-helix structure of the Psq motif, but it lacks the conserved sequence signatures of the classical eukaryotic DNA-binding motifs. Psq may thus represent the prototype of a new family of DNA-binding proteins.