RESUMO
A two-stage Partial Nitritation (PN)/Anammox process was carried out at lab-scale conditions to treat reject water from a municipal WWTP. PN was achieved in a granular SBR obtaining an effluent with a NH4(+)-N/NO2(-)-N molar ratio around 1.0. The microbial characterization of this reactor revealed a predominance of Betaproteobacteria, with a member of Nitrosomonas as the main autotrophic ammonium oxidizing bacterium (AOB). Nitrite oxidizing bacteria (NOB) were under the detection limit of 16S rRNA gene pyrosequencing, indicating their effective inhibition. The effluent of the PN reactor was fed to an Anammox SBR where stable operation was achieved with a NH4(+)-N:NO2(-)-N:NO3(-)-N stoichiometry of 1:1.25:0.14. The deviation to the theoretical stoichiometry could be attributed to the presence of heterotrophic biomass in the Anammox reactor (mainly members of Chlorobi and Chloroflexi). Planctomycetes accounted for 7% of the global community, being members of Brocadia (1.4% of the total abundance) the main anaerobic ammonium oxidizer detected.
Assuntos
Compostos de Amônio/metabolismo , Reatores Biológicos/microbiologia , Consórcios Microbianos/fisiologia , Eliminação de Resíduos Líquidos/métodos , Processos Autotróficos , Bactérias/genética , Bactérias/metabolismo , Biomassa , Consórcios Microbianos/genética , Nitritos/metabolismo , Nitrogênio/metabolismo , RNA Ribossômico 16S/genética , Eliminação de Resíduos Líquidos/instrumentaçãoRESUMO
An anaerobic membrane bioreactor and aerobic granulation technologies were tested at laboratory scale to treat winery wastewater, which is characterised by a high and variable biodegradable organic load. Both technologies have already been tested for alcohol fermentation wastewaters, but there is a lack of data relating to their application to winery wastewater treatment. The anaerobic membrane bioreactor, with an external microfiltration module, was started up for 230 days, achieving a biogas production of up to 0.35 L CH4L(-1)d(-1) when 1.5 kg COD m(-3)d(-1) was applied. Average flux was 10.5 L m(-2) h(-1) (LMH), obtaining a treated effluent free of suspended solids and a chemical oxygen demand (COD) concentration lower than 100 mg COD L(-1). In contrast, the aerobic granular sequencing batch reactor coped with 15 kg COD m(-3)d(-1), but effluent quality was slightly worse. Aerobic granulation was identified as a suitable technique to treat this kind of wastewater due to excellent settleability, high biomass retention and a good ability to handle high organic loads and seasonal fluctuations. However, energy generation from anaerobic digestion plays an important role, favouring anaerobic membrane bioreactor application, although it was observed to be sensitive to sudden load fluctuations, which led to a thorough pH control and alkali addition.
Assuntos
Eliminação de Resíduos Líquidos/métodos , Vinho , Análise da Demanda Biológica de Oxigênio , Reatores Biológicos , Resíduos IndustriaisRESUMO
Municipal solid waste is broadly generated in everyday activities and its treatment is a global challenge. Total solids (TS) and volatile solids (VS) are typical control parameters measured in biological treatments. In this study, the TS and VS were determined using the standard methods, as well as introducing some variants: (i) the drying temperature for the TS assays was 105°C, 70°C and 50°C and (ii) the VS were determined using different heating ramps from room tempature to 550°C. TS could be determined at either 105°C or 70°C, but oven residence time was tripled at 70°C, increasing from 48 to 144 h. The VS could be determined by smouldering the sample (where the sample is burnt without a flame), which avoids the release of fumes and odours in the laboratory. However, smouldering can generate undesired pyrolysis products as a consequence of carbonization, which leads to VS being underestimated. Carbonization can be avoided using slow heating ramps to prevent the oxygen limitation. Furthermore, crushing the sample cores decreased the time to reach constant weight and decreased the potential to underestimate VS.
Assuntos
Resíduos Sólidos , Dessecação , Temperatura Alta , VolatilizaçãoRESUMO
The impact of food waste (FW) composition on co-fermentation performance was studied to elucidate if adjusting FW composition can be used to drive the fermentation yield and profile, which is relevant for biorefinery applications. First, the impact of individual FW components (i.e., fruit, vegetables, pasta, rice, meat, fish, and cellulose) was assessed. Subsequently, the effect of mixing a protein-rich component and a carbohydrate-rich component was studied (i.e., fish/fruit and fish/cellulose, and meat/rice and meat/vegetable). All experiments were carried out in mesophilic batch assays using waste activated sludge (WAS) as main substrate, the same mixture ratio (70 % WAS +30 % FW on VS basis), and no pH control. Results showed that each FW component had a distinct effect on VFA yield and profile, with protein-rich components reaching the highest VFA yields; 502 and 442 mgCOD/gVS for WAS/Fish and WAS/Meat, respectively. A positive interaction on VFA yield was observed when mixing a protein-rich and a carbohydrate-rich component. This interaction was not proportional to the co-substrates proportion in the mixtures. On the other hand, the VFA profile was clearly driven by the components in the mixture, including both WAS and FW composition. Overall, these results indicate that predicting the VFA yield of WAS/FW co-fermentation is not just related to FW composition, but FW composition could be used to adjust the VFA profile to a certain extent.
Assuntos
Eliminação de Resíduos , Esgotos , Ácidos , Anaerobiose , Reatores Biológicos , Carboidratos , Celulose , Ácidos Graxos Voláteis , Fermentação , Alimentos , Eliminação de Resíduos/métodos , VerdurasRESUMO
A gas-permeable membrane (GPM) contactor was used to recover ammoniacal nitrogen from a synthetic and a biowaste fermentation broth under different pH (from 6 to 11) and temperatures (35 and 55 °C). Ammonia mass transfer constant (Km) increased as pH and temperature increased. For synthetic broth, pH 10 provided the best results, when considering the Km (9.2·10-7 m·s-1) and the reagents consumption (1.0 mol NaOH·mol-1 TAN and 0.6 mol H2SO4·mol-1 TAN). Biowaste fermentation generated a broth with a high concentration of ammoniacal nitrogen (4.9 g N·L-1) and volatile fatty acids (VFA) (41.1 g COD·L-1). Experiments using the biowaste broth showed a lower Km (5.0·10-7 m·s-1 at pH 10) than the synthetic broth, related to the solution matrix and other species interference. VFAs were not detected in the trapping solution. Overall, these results show that GPM is a suitable technology to efficiently separate ammoniacal nitrogen and VFA from fermentation broths.
Assuntos
Amônia , Reatores Biológicos , Ácidos Graxos Voláteis , Fermentação , Concentração de Íons de Hidrogênio , Nitrogênio , EsgotosRESUMO
It is a fact that the rapid increase of biodiesel production over the last years has resulted in the generation of large and constant amounts of glycerine, which is causing an oversupply problem. Since glycerine is a biodegradable organic compound exempt of nitrogen, it can be applied as a co-substrate in the anaerobic digestion process of pig manure (PM). In order to analyze the feasibility of a mixture of pig manure and glycerine in anaerobic processes and to define the effect originated by the nitrogen limitation when large amounts of glycerine are added, several biodegradability batch tests were performed with different mixtures. These were named as: 100% PM, 80% PM, 60% PM, 40% PM and 20% PM, in pig manure wet weight-basis. Furthermore, a modified model based on anaerobic digestion model no.1 (ADM1) was used to simulate the methane production profiles for the mixtures tested. Specifically, both experimental and model results show the power of the co-digestion technology. In particular, the mixture of 80% PM produced the highest methane production with 215 mL CH(4) g(-1) COD, almost 125% more methane than when pig manure was mono-digested. In contrast, the one with 20% PM was clearly inhibited by the volatile fatty acid due to the low nitrogen concentration of the mixture. In addition, the specific methane production predicted by the model was in good agreement with the experimental results, although in some samples the shape of the profiles did not match perfectly. Moreover, the modified ADM1 appears to be a useful tool to predict the methane production and the limitations related to the lack/excess of nitrogen during the co-digestion process of pig manure and glycerine.
Assuntos
Glicerol/metabolismo , Esterco , Metano/análise , Anaerobiose , Animais , Biodegradação Ambiental , Cromatografia Gasosa , Modelos Biológicos , SuínosRESUMO
Granular sludge formation was performed in a laboratory scale Sequencing Batch Reactor (SBR) fed with supernatant of anaerobic digestion of sewage sludge. This effluent was concentrated progressively in order to enhance biomass capacity without inhibiting it. During the first part of the study, ammonium nitrogen was converted to nitrate, so conventional nitrification took place. When a nitrogen load of 0.8 g N L(-1) d(-1) was treated, the effluent concentration of nitrite started to increase while the nitrate concentration decreased until it disappeared. So, partial nitrification was achieved. At the end of this study, a nitrogen load of 1.1 g N L(-1) d(-1) was treated obtaining an effluent with 50% ammonium and 50% nitrite. The volatile suspended solids concentration in the reactor reached 10 g VSS L(-1). At this point the granule morphology was quite round and no filamentous bacteria was observed. The Feret's diameter was in the range between 1 and 6 mm with an average value of 4.5 mm. Roundness value was all the time higher than 0.7. Granule density increased during the experimental period, obtaining a final value of 7.0 g L(-1).
Assuntos
Nitrificação , Esgotos/química , Água/química , Aerobiose , Anaerobiose , Biomassa , Reatores Biológicos/microbiologia , Nitratos/análise , Nitritos/análise , Nitrogênio/análise , Compostos de Amônio Quaternário/análise , Eliminação de Resíduos LíquidosRESUMO
This study investigated waste activated sludge (WAS) and food waste (FW) co-fermentation in batch assays to produce carboxylic acids. Three mixtures (50%, 70% and 90% WAS in VS basis) were studied under different conditions: with and without extra alkalinity, and with and without WAS auto-hydrolysis pre-treatment. All tests were carried out at 35 °C, without pH adjustment and without external inoculum. Experimental results showed that co-fermentation yields, including volatile fatty acids and lactic acid, were always higher than WAS and FW mono-fermentation yields (ca. 100 and 80 mgCOD/gVS, respectively). Co-fermentation yields increased as the proportion of FW in the mixture increased, indicating that the improvement was primarily due to a higher FW degradation under co-fermentation conditions. The maximum co-fermentation yield was on average 480 mgCOD/gVS for the WAS/FW_50/50 mixture. The importance of pH on co-fermentation performance was evident in the experiments carried out with extra alkalinity, which showed that the proportion of WAS in the mixture should be high enough to keep the pH above 5.0. However, fermenters operational conditions should also prevent the enrichment of acetic acid consuming microorganisms. WAS auto-hydrolysis pre-treatment did not enhance co-fermentation yields but showed minor kinetic improvements. Regarding the product profile, butyric acid was enriched as the proportion of FW in the mixture increased and the concomitant pH decreased to the detriment of propionic acid. Propionic acid prevailed under neutral pH in the WAS mono-fermentation and the WAS/FW_90/10 mixture.
Assuntos
Eliminação de Resíduos , Esgotos , Reatores Biológicos , Ácidos Carboxílicos , Ácidos Graxos Voláteis , Fermentação , Alimentos , Concentração de Íons de HidrogênioRESUMO
Aerobic granular sludge was cultivated in a sequencing batch reactor (SBR) in order to remove the organic matter present in winery wastewater. The formation of granules was performed using a synthetic substrate. The selection parameter was the settling time, as well as the alternation of feast-famine periods, the air velocity and the height/diameter ratio of the reactor. After 10 days of operation under these conditions, the first aggregates could be observed. Filamentous bacteria were still present in the reactor but they disappeared progressively. During the start-up, COD loading was increased from 2.7 to 22.5 kg COD/(m(3) day) in order to obtain a feast period between 30 and 60 minutes. At this point, granules were quite round, with a particle diameter between 3.0 and 4.0 mm and an average density of 6 g L(-1). After 120 days of operation, synthetic media was replaced by real winery wastewater, with a COD loading of 6 kg COD/(m(3) day). The decrease of the organic load implied a reduction of the aggregate diameter and a density increase up to 13.2 g L(-1). The effluent was free of organic matter and the solids concentration in the reactor reached 6 g VSS L(-1).
Assuntos
Reatores Biológicos/microbiologia , Resíduos Industriais/análise , Eliminação de Resíduos Líquidos/métodos , Purificação da Água/métodos , Vinho , Aerobiose , Indústria Alimentícia/métodos , Microscopia Eletrônica de Varredura , Oxigênio/isolamento & purificação , Esgotos/microbiologiaRESUMO
The anaerobic digestion of the organic fraction of municipal solid wastes is taking increasing importance in the recent years. The main problem of some anaerobic digestion process is the large quantity of ammonia that is released, especially when high solid digestion is implemented. A fraction of the supernatant is treated and the remaining is recirculated to maintain the reactor in the optimum solids concentration. The question arising is if this recirculation stream should also be treated to improve biogas production. However, when doing the latter the quantity of ammonia inside the reactor increases too which could lead to inhibit the reactor operation. In this paper it appears that not only free ammonia affects the methanogenic fermentation but also ammonium ion concentration. Biogas production profiles are estimated using the Gompertz model. On the other hand, inhibition constants are fitted using a non-competitive inhibition model equation Thus, 50% inhibition of biomethane production was observed at level of 215 and 468 mg NH3_N/L under mesophilic and thermophilic conditions. However, the methane generation under mesophilic and thermophilic conditions was reduced by 50% when ammonium ion reach concentrations of 3,860 and 5,600 mg NH4+_N/L respectively. Under mesophilic conditions, pH higher than 7 impacted the methanogens bacteria negatively. This threshold pH limit, is variable under thermophilic conditions, depending on the total ammonia concentration.
Assuntos
Amônia/química , Amônia/metabolismo , Eliminação de Resíduos/métodos , Esgotos/química , Anaerobiose , Concentração de Íons de HidrogênioRESUMO
The supernatant from mesophilic anaerobic digestion of piggery wastewater is characterised by a high amount of COD (4.1 g COD L(-1)), ammonium (2.3g NH(4)(+)-NL(-1)) and suspended solids (2.5 g SS L(-1)). This effluent can be efficiently treated by means of a Sequencing Batch Reactor (SBR) strategy for biological COD, SS and nitrogen removal including a Coagulation/Flocculation step. Total COD and SS reduction yields higher than 66% and 74%, respectively, and a total nitrogen removal (via nitrite) of more than 98% were reached when working with HRT 2.7 days, SRT 12 days, temperature 32 degrees C, three aerobic/anoxic periods, without external control of pH and under limited aeration flow. The inhibition of nitrite oxidizing biomass was achieved by the working free ammonia concentration and the restricted air supply (dissolved oxygen concentration below 1 mg O(2)L(-1)). Since a part of the total COD was colloidal and/or refractory, a Coagulation/Flocculation step was implemented inside the SBR operating strategy to meet a suitable effluent quality to be discharged. Several Jar-Tests demonstrated that the optimal concentration of FeCl(3) was 800 mg L(-1). A respirometric assay showed that this coagulant dosage did not affect the biological activity of nitrifying/denitrifying biomass.
Assuntos
Biomassa , Esgotos , Suínos , Eliminação de Resíduos Líquidos , Anaerobiose , Criação de Animais Domésticos/economia , Animais , Custos e Análise de Custo , Floculação , Óleos Combustíveis , Meios de Transporte/economia , Estados Unidos , Poluentes da Água/análiseRESUMO
The application of the Anammox process has been usually focused on the treatment of wastewater with temperatures around 30 degrees C in order to operate under optimum conditions. In this work, the feasibility of the application of the Anammox process at lower temperatures has been tested. First, the short-term effects of temperature on the Anammox biomass were studied using batch tests. An activation energy of 63 kJ mol(-1) was calculated and the maximum activity was found at 35-40 degrees C. Activity tests done at 45 degrees C showed an irreversible loss of the activity due to the biomass lysis. A SBR was operated at different temperatures (from 30 to 15 degrees C) to determine the long-term effects. The system was successfully operated at 18 degrees C but when temperature was decreased to 15 degrees C, nitrite started to accumulate and the system lost its stability. Adaptation of biomass to low temperatures was observed when the specific activities obtained during first batch tests are compared to those obtained during the operation of the SBR.
Assuntos
Reatores Biológicos , Compostos de Amônio Quaternário/metabolismo , Poluentes Químicos da Água/metabolismo , Anaerobiose , Bactérias/metabolismo , Nitritos/metabolismo , Oxirredução , Temperatura , Eliminação de Resíduos LíquidosRESUMO
An efficient biological treatment to treat reject water from anaerobic digestion of wastewater sludge is the SHARON denitrification process, which takes place in a chemostat reactor, where aerobic/anoxic periods are alternated under specific hydraulic retention time (HRT) and temperature conditions that favor ammonium oxidizers growth and ensure the total washout of nitrite oxidizers, achieving the biological nitrogen removal over nitrite. An optimized performance of this process to treat Spanish reject water was obtained using methanol and working at an HRT of 2 days, 33 degrees C, and cycle length of 2 hours. Supernatant of hydrolyzed primary sludge was tested to denitrify. Because biochemical oxygen demand was not extremely high in the primary sludge, the fluid dynamics of the system were changed, with respect to the strategy with methanol, but maintaining the reject water influent flowrate. The use of hydrolyzed primary sludge improved the process efficiency, because the alkalinity present in the primary sludge buffered the process until an optimum pH range.
Assuntos
Nitritos/metabolismo , Nitrogênio/metabolismo , Eliminação de Resíduos Líquidos/métodos , Purificação da Água/métodos , Aerobiose , Anaerobiose , Biodegradação Ambiental , Reatores Biológicos , HumanosRESUMO
SBR technology is used to treat the supernatant from mesophilic anaerobic digestion of piggery wastewater. The novelty of the treatment consists in the use of a final coagulation/flocculation step inside the SBR cycle to reach the legal COD effluent standard. The pH changes introduced by the use of FeCl(3) do not affect the nitrifying activity. The SBR treatment includes a strategy to the control of oxygen supply and ammonia concentrations inside the digester to favor the biological nitrogen removal over nitrite, which makes the process more economical. The influence of several of these parameters on the AOB biomass activity is studied in this paper.
Assuntos
Esterco/análise , Nitritos/isolamento & purificação , Nitrogênio/isolamento & purificação , Aerobiose , Amônia/análise , Anaerobiose , Animais , Biomassa , Digestão , Concentração de Íons de Hidrogênio , Cinética , Suínos , Eliminação de Resíduos Líquidos/métodosRESUMO
This paper focuses on the study of high ammonium concentrated wastewater with SBR reactors. Four type of wastewaters, landfill leachates (T=20 degrees C) and the reject water (T=35 degrees C) coming from mesophilic anaerobic digesters of sewage sludge, pig slurry and organic fraction of municipal solid waste (OFMSW), were studied in four SBR during 6 months. The removal of nitrogen was done in all the cases with nitrification/denitrification via nitrite obtaining high removal nitrogen conversions for the three types of reject water (0.75-0.85 kg N day(-1) m(-3)) and lower for landfill leachates due to temperature requirements (0.3 kg N day(-1) m(-3)).
Assuntos
Reatores Biológicos , Compostos de Amônio Quaternário/química , Purificação da Água/instrumentação , Purificação da Água/métodos , Agricultura , Anaerobiose , Animais , Nitritos , Esgotos/química , Suínos , Temperatura , Eliminação de Resíduos Líquidos/instrumentação , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da ÁguaRESUMO
In many anaerobic digestion processes for the treatment of the sludge produced in wastewater treatment plants, the hydrolysis of the organic matter has been identified as the rate limiting step. This study is focused on the effect of ultrasonic pretreatment of raw sewage sludge before being fed to the mesophilic and the thermophilic anaerobic digestion. From particle size reduction, COD disintegration degree and biodegradability test, 11,000kJ/kg TS was estimated as the optimal specific energy in ultrasonic pretreatment. Moreover, the use of pretreated sludge improved significantly the COD removal efficiency and biogas production in lab-scale anaerobic digesters when compared with the performance without pretreatment, specially under mesophilic conditions. During ultrasonic pretreatment, the diffusion of polycyclic aromatic hydrocarbons (PAH) compounds to the aqueous phase was stated by a reduction in the pretreated sludge micropollutants content. With sonication, naphthalene was better removed than without this pretreatment, particularly in the mesophilic digester. However, pyrene removal remained at same efficiency level with and without ultrasonic pretreatment.
Assuntos
Anaerobiose , Biodegradação Ambiental , Naftalenos/química , Pirenos/química , Ultrassom , Bactérias Anaeróbias/metabolismo , Reatores Biológicos , Esgotos , Temperatura , Eliminação de Resíduos Líquidos/métodosRESUMO
The aim of this study was the operation and model description of a sequencing batch reactor (SBR) for biological nitrogen removal (BNR) from a reject water (800-900 mg NH(4)(+)-NL(-1)) from a municipal wastewater treatment plant (WWTP). The SBR was operated with three cycles per day, temperature 30 degrees C, SRT 11 days and HRT 1 day. During the operational cycle, three alternating oxic/anoxic periods were performed to avoid alkalinity restrictions. Oxygen supply and working pH range were controlled to achieve the BNR via nitrite, which makes the process more economical. Under steady state conditions, a total nitrogen removal of 0.87 kg N (m(3)day)(-1) was reached. A four-step nitrogen removal model was developed to describe the process. This model enlarges the IWA activated sludge models for a more detailed description of the nitrogen elimination processes and their inhibitions. A closed intermittent-flow respirometer was set up for the estimation of the most relevant model parameters. Once calibrated, model predictions reproduced experimental data accurately.
Assuntos
Reatores Biológicos , Nitritos/química , Nitrogênio/química , Poluentes Químicos da Água/química , Purificação da Água/métodos , Bactérias/crescimento & desenvolvimento , Bactérias/metabolismo , Biodegradação Ambiental , Biomassa , Calibragem , Concentração de Íons de Hidrogênio , Cinética , Modelos Teóricos , Nitritos/metabolismo , Nitrogênio/metabolismo , Oxigênio/química , Água/química , Microbiologia da Água , Poluentes Químicos da Água/metabolismo , Purificação da Água/economia , Purificação da Água/instrumentaçãoRESUMO
An optimal sequencing batch reactor (SBR) strategy is proposed for Biological Nitrogen Removal (BNR) via nitrite of reject water (800-900 NH4+-N mg l(-1)) from mesophilic (35 degrees C) anaerobic sludge digester of a Spanish Municipal Wastewater Treatment Plant (WWTP). Two lab-scale SBR with control of temperature were studied with external COD addition for denitrification which was necessary due to the lack of readily biodegradable carbon source. Process kinetics were compared through the specific Ammonium Uptake Rate (sAUR) finding the appropriate operational sequences when working at 32 degrees C and 8 hour cycle length. Every operating cycle was carried out with a sludge retention time of 11 days, hydraulic retention time around 1 day and 2500+/-250 mg VSS l(-1). In order to avoid nitrate formation and thus save costs, the oxygen concentration was maintained below 1 mg V1 during aerobic periods and pH remained within an optimal range (7.5-9) alternating different aerobic-anoxic subcycles inside the operational cycle. With this strategy, the range of alkalinity could be controlled avoiding the addition of external additives and nitrite accumulation was prevented. Therefore, the reached sAUR was 22 mg NH4+-N g)-1) VSS h(-1) and the total nitrogen removal was 0.8 kg N(d m(3))(-1).
Assuntos
Nitratos , Nitritos , Nitrogênio/isolamento & purificação , Esgotos/microbiologia , Purificação da Água/métodos , Aerobiose , Amônia/química , Amônia/metabolismo , Carbono/química , Carbono/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Nitratos/química , Nitratos/metabolismo , Nitritos/química , Nitritos/metabolismo , Nitrogênio/metabolismo , Oxigênio/química , Oxigênio/metabolismo , Esgotos/química , Espanha , Temperatura , Purificação da Água/instrumentaçãoRESUMO
A comparison between three feasible ways of developing Biological Nitrogen Removal (BNR) via nitrite to treat real reject water of 800-900 mg NH4(+)-N l(-1) is proposed. A Sequencing Batch Reactor (SBR) and a chemostat SHARON (Single reactor High activity Ammonium Removal Over Nitrite) continuous reactor were operated. In the SBR operation 0.8 kg N (d m3)(-1) was achieved, whereas in SHARON/denitrification the removal reached was 0.4 kg N (d m3)(-1). SHARON was also developed with partial nitrification of ammonium in order to obtain a stream ready for Anammox (Anaerobic Ammonia Oxidation) process obtaining an effluent with an average composition of 400 mg NO2(-)-N l(-1) and 350 mg NH4(-)-N l(-1).
Assuntos
Nitritos/metabolismo , Eliminação de Resíduos Líquidos/métodos , Reatores Biológicos , Nitratos/análise , Nitratos/metabolismo , Nitritos/análise , Compostos de Amônio Quaternário/análise , Compostos de Amônio Quaternário/metabolismoRESUMO
Anaerobic digestion for the treatment of sludge in wastewater treatment plants has been reported to produce a low organic loaded effluent with an acceptable economic cost. But in the last years, new regulations and the increasing sludge production invite us to find an alternative and/or to improve the process efficiency. Moreover, the use of the effluent as fertilizer in agriculture imposes more restrictions on digestion process product and its micropollutant contents to protect the environment. In this study, a performance of the anaerobic digestion under mesophilic and thermophilic conditions at different hydraulic retention times (HRT) is assessed and the removal efficiencies of two important compounds or family compounds (Polycyclic Aromatic Hydrocarbons, PAH, and Di-2-(Ethyl-Hexyl)-Phthalate, DEHP) are evaluated. A positive effect of thermophilic temperature was observed on both micropollutants' biodegradation. However, HRT effect also had an important role for DEHP and low molecular weighted PAH removal.