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1.
Molecules ; 29(1)2023 Dec 29.
Artigo em Inglês | MEDLINE | ID: mdl-38202779

RESUMO

Amphibians are widely known as a prolific source of bioactive metabolites. In this work, we isolated and characterized compounds with antiparasitic activity from the oocytes of the toad Rhinella alata collected in Panama. Bio-guided isolation and structural elucidation were carried out using chromatographic and spectroscopic techniques, respectively. The organic extract was subjected to solid phase extraction followed by HPLC purification of the fraction with in vitro activity against Trypanosoma cruzi trypomastigotes. Seven steroids (1-7) of the bufadienolide family were isolated, and their structures were determined using NMR and MS analyses; of these 19-formyl-dyscinobufotalin, (3) is reported as a new natural product. Compounds 1 and 3-7 resulted in a good anti-trypanosomal activity profile. Among these, 16ß-hydroxyl-hellebrigenin (1) and bufalin (7) showed significant selectivity values of >5 and 2.69, respectively, while the positive control benznidazole showed a selectivity of 18.81. Furthermore, molecular docking analysis showed compounds 1, 3 and 7 interact through H-bonds with the amino acid residues GLN-19, ASP-158, HIS-159 and TRP-177 from cruzipain at the catalytic site. Given the lack of therapeutic options to treat American trypanosomiasis, this work can serve as the basis for further studies that aim for the development of bufadienolides or their derivatives as drugs against Chagas disease.


Assuntos
Bufanolídeos , Doença de Chagas , Trypanosoma cruzi , Animais , Bufonidae , Simulação de Acoplamento Molecular , Oócitos , Bufanolídeos/farmacologia , Doença de Chagas/tratamento farmacológico
2.
J Nat Prod ; 84(5): 1434-1441, 2021 05 28.
Artigo em Inglês | MEDLINE | ID: mdl-33979168

RESUMO

In this study, eight natural isocoumarins (1-8) were isolated from a marine-derived Exserohilum sp. fungus. To explore their structure-activity relationship and discover potent antimalarial leads, a small library of 22 new derivatives (1a-1n, 2a, 3a-3c, 4a-4c, and 7a) were semisynthesized by varying the substituents of the aromatic ring and the aliphatic side chains. The natural compound (1) and three semisynthetic derivatives (1d, 1n, and 2a), possessing an all-cis stereochemistry, exhibited strong antiplasmodial activity with IC50 values of 1.1, 0.8, 0.4, and 2.6 µM, respectively. Mechanism studies show that 1n inhibits hemozoin polymerization and decreases the mitochondrial membrane potential but also inhibits P. falciparum DNA gyrase. 1n not only combines different mechanisms of action but also exhibits a high therapeutic index (CC50/IC50 = 675), high selectivity, and a notable drug-like profile.


Assuntos
Antimaláricos/farmacologia , Ascomicetos/química , Isocumarinas/farmacologia , Animais , Antozoários/microbiologia , Antimaláricos/síntese química , Organismos Aquáticos/química , China , Chlorocebus aethiops , DNA Girase , Hemeproteínas , Isocumarinas/síntese química , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Estrutura Molecular , Plasmodium falciparum/efeitos dos fármacos , Plasmodium falciparum/enzimologia , Espécies Reativas de Oxigênio/metabolismo , Relação Estrutura-Atividade , Inibidores da Topoisomerase II/farmacologia , Células Vero
3.
Molecules ; 26(14)2021 Jul 12.
Artigo em Inglês | MEDLINE | ID: mdl-34299492

RESUMO

Toads in the family Bufonidae contain bufadienolides in their venom, which are characterized by their chemical diversity and high pharmacological potential. American trypanosomiasis is a neglected disease that affects an estimated 8 million people in tropical and subtropical countries. In this research, we investigated the chemical composition and antitrypanosomal activity of toad venom from Rhinella alata collected in Panama. Structural determination using mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy led to the identification of 10 bufadienolides. Compounds identified include the following: 16ß-hydroxy-desacetyl-bufotalin-3-adipoyl-arginine ester (1), bufotalin (2), 16ß-hydroxy-desacetyl-bufotalin-3-pimeloyl-arginine ester (3), bufotalin-3-pimeloyl-arginine ester (4), 16ß-hydroxy-desacetyl-bufotalin-3-suberoyl-arginine ester (5), bufotalin-3-suberoyl-arginine ester (6), cinobufagin-3-adipoyl-arginine ester (7), cinobufagin-3-pimeloyl-arginine ester (8), cinobufagin-3-suberoyl-arginine ester (9), and cinobufagin (10). Among these, three new natural products, 1, 3, and 5, are described, and compounds 1-10 are reported for the first time in R. alata. The antitrypanosomal activity assessed in this study revealed that the presence of an arginyl-diacid attached to C-3, and a hydroxyl group at C-14 in the structure of bufadienolides that is important for their biological activity. Bufadienolides showed cytotoxic activity against epithelial kidney Vero cells; however, bufagins (2 and 10) displayed low mammalian cytotoxicity. Compounds 2 and 10 showed activity against the cancer cell lines MCF-7, NCI-H460, and SF-268.


Assuntos
Antiprotozoários/farmacologia , Bufanolídeos/farmacologia , Bufonidae/metabolismo , Pele/metabolismo , Venenos de Anfíbios/metabolismo , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Chlorocebus aethiops , Humanos , Células MCF-7 , Espectrometria de Massas/métodos , Panamá , Trypanosoma cruzi , Células Vero
4.
J Nat Prod ; 81(1): 211-215, 2018 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-29327931

RESUMO

We reported previously the discovery of the potent antimalarial 40-membered macrolide bastimolide A (1) from the tropical marine cyanobacterium Okeania hirsute. Continued investigation has led to the discovery of a new analogue, bastimolide B (2), a 24-membered polyhydroxy macrolide with a long aliphatic chain and unique terminal tert-butyl group. Its complete structure was determined by a combination of extensive spectroscopic methods and comparative analysis of its methanolysis products with those of bastimolide A. A methanolysis mechanism for bastimolide A is proposed, and one unexpected isomerization product of the C2-C3 double bond, 2-(E)-bastimolide A (3), was obtained. Bastimolide B (2) showed strong antimalarial activity against chloroquine-sensitive Plasmodium falciparum strain HB3. A preliminary investigation of the structure-activity relationship based on six analogues revealed the importance of the double bond as well as the 1,3-diol and 1,3,5-triol functionalities.


Assuntos
Antimaláricos/química , Organismos Aquáticos/química , Macrolídeos/química , Antimaláricos/farmacologia , Cloroquina/química , Cloroquina/farmacologia , Cianobactérias/química , Macrolídeos/farmacologia , Plasmodium falciparum/efeitos dos fármacos , Relação Estrutura-Atividade
5.
Molecules ; 23(9)2018 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-30158478

RESUMO

Chemical examination of the octocoral-associated Bacillus species (sp.) DT001 led to the isolation of pumilacidins A (1) and C (2). We investigated the effect of these compounds on the viability of Plasmodium falciparum and the mechanism of pumilacidin-induced death. The use of inhibitors of protein kinase C (PKC) and phosphoinositide 3-kinase (PI3K) was able to prevent the effects of pumilacidins A and C. The results indicated also that pumilacidins inhibit parasite growth via mitochondrial dysfunction and decreased cytosolic Ca2+.


Assuntos
Antimaláricos/farmacologia , Bacillus/química , Peptídeos/farmacologia , Plasmodium falciparum/crescimento & desenvolvimento , Animais , Antozoários/microbiologia , Antimaláricos/química , Cálcio/metabolismo , Mitocôndrias/efeitos dos fármacos , Estrutura Molecular , Peptídeos/química , Plasmodium falciparum/efeitos dos fármacos , Plasmodium falciparum/metabolismo , Inibidores de Proteínas Quinases/farmacologia
6.
J Nat Prod ; 80(6): 1827-1836, 2017 06 23.
Artigo em Inglês | MEDLINE | ID: mdl-28535042

RESUMO

A family of 2,2-dimethyl-3-hydroxy-7-octynoic acid (Dhoya)-containing cyclic depsipeptides, named dudawalamides A-D (1-4), was isolated from a Papua New Guinean field collection of the cyanobacterium Moorea producens using bioassay-guided and spectroscopic approaches. The planar structures of dudawalamides A-D were determined by a combination of 1D and 2D NMR experiments and MS analysis, whereas the absolute configurations were determined by X-ray crystallography, modified Marfey's analysis, chiral-phase GCMS, and chiral-phase HPLC. Dudawalamides A-D possess a broad spectrum of antiparasitic activity with minimal mammalian cell cytotoxicity. Comparative analysis of the Dhoya-containing class of lipopeptides reveals intriguing structure-activity relationship features of these NRPS-PKS-derived metabolites and their derivatives.


Assuntos
Antiparasitários/isolamento & purificação , Antiparasitários/farmacologia , Cianobactérias/química , Depsipeptídeos/isolamento & purificação , Depsipeptídeos/farmacologia , Animais , Antiparasitários/química , Cromatografia Líquida de Alta Pressão , Cristalografia por Raios X , Depsipeptídeos/química , Ensaios de Seleção de Medicamentos Antitumorais , Lipopeptídeos/química , Biologia Marinha , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Papua Nova Guiné , Peptídeos Cíclicos/química , Relação Estrutura-Atividade
7.
J Nat Prod ; 80(8): 2328-2334, 2017 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-28783331

RESUMO

A recent untargeted metabolomics investigation into the chemical profile of 10 organic extracts from cf. Symploca spp. revealed several interesting chemical leads for further natural product drug discovery. Subsequent target-directed isolation efforts with one of these, a Panamanian marine cyanobacterium cf. Symploca sp., yielded a phenethylamide metabolite that terminates in a relatively rare gem-dichlorovinylidene moiety, caracolamide A (1), along with a known isotactic polymethoxy-1-alkene (2). Detailed NMR and HRESIMS analyses were used to determine the structures of these molecules, and compound 1 was confirmed by a three-step synthesis. Pure compound 1 was shown to have in vitro calcium influx and calcium channel oscillation modulatory activity when tested as low as 10 pM using cultured murine cortical neurons, but was not cytotoxic to NCI-H460 human non-small-cell lung cancer cells in vitro (IC50 > 10 µM).


Assuntos
Cianobactérias/química , Canais Iônicos/química , Fenetilaminas/química , Carcinoma Pulmonar de Células não Pequenas , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Neoplasias Pulmonares , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Fenetilaminas/isolamento & purificação , Fenetilaminas/farmacologia
8.
Pharm Biol ; 54(11): 2723-2731, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27181630

RESUMO

CONTEXT: Marine cyanobacteria offer a robust resource for natural products drug discovery due to the secondary metabolites they produce. OBJECTIVE: To identify novel cyanobacterial compounds that exhibit CNS psychoactive effects. MATERIALS AND METHODS: Cyanobacteria were collected from Las Perlas Archipelago, Panama and subjected to dichloromethane/methanol extraction and fractionation by column chromatography before being screened for affinity against a panel of CNS targets. A 50:50 ethyl acetate:methanol fraction of one cyanobacterial extract (2064H) was subjected to HPLC and the major peak was isolated (2064H3). At a dose of 20 µg per animal, 2064H and 2064H3 were tested in mice using behavioral assays that included the forced swim, open field and formalin tests. RESULTS: 2064H was shown to bind to the serotonin 2C (5-HT2C) receptor, a known target for depression and pain treatment. 2064H showed 59.6% inhibition of binding of [3H]-mesulergine with an IC50 value of 179 ng/mL and did not show inhibition of binding greater than 45% with any other receptors tested. Both 2064H and 2064H3 decreased immobility time in the first minute of the tail suspension test. 2064H increased time, distance and number of entries in the center region in the first half of the open field test. 2064H increased overall nocifensive behaviors in the formalin test. DISCUSSION AND CONCLUSION: Overall, manipulating the 5-HT2C receptor with these receptor-specific ligands derived from cyanobacteria altered pain, depression and anxiety-like behaviors, illustrating the importance of this receptor in affective behaviors. These results demonstrate the potential of cyanobacteria as a source for CNS active compounds.


Assuntos
Cianobactérias/metabolismo , Psicotrópicos/farmacologia , Receptor 5-HT2C de Serotonina/efeitos dos fármacos , Animais , Ansiolíticos/farmacologia , Antidepressivos/farmacologia , Cromatografia Líquida de Alta Pressão , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Atividade Motora/efeitos dos fármacos
9.
Biochim Biophys Acta ; 1840(6): 2032-41, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24556123

RESUMO

BACKGROUND: Malaria is an extremely devastating disease that continues to affect millions of people each year. A distinctive attribute of malaria infected red blood cells is the presence of malarial pigment or the so-called hemozoin. Hemozoin is a biocrystal synthesized by Plasmodium and other blood-feeding parasites to avoid the toxicity of free heme derived from the digestion of hemoglobin during invasion of the erythrocytes. SCOPE OF REVIEW: Hemozoin is involved in several aspects of the pathology of the disease as well as in important processes such as the immunogenicity elicited. It is known that the once best antimalarial drug, chloroquine, exerted its effect through interference with the process of hemozoin formation. In the present review we explore what is known about hemozoin, from hemoglobin digestion, to its final structural analysis, to its physicochemical properties, its role in the disease and notions of the possible mechanisms that could kill the parasite by disrupting the synthesis or integrity of this remarkable crystal. MAJOR CONCLUSIONS: The importance and peculiarities of this biocrystal have given researchers a cause to consider it as a target for new antimalarials and to use it through unconventional approaches for diagnostics and therapeutics against the disease. GENERAL SIGNIFICANCE: Hemozoin plays an essential role in the biology of malarial disease. Innovative ideas could use all the existing data on the unique chemical and biophysical properties of this macromolecule to come up with new ways of combating malaria.


Assuntos
Hemeproteínas/fisiologia , Malária/tratamento farmacológico , Animais , Cloroquina/farmacologia , Cristalização , Hemeproteínas/antagonistas & inibidores , Hemeproteínas/química , Humanos , Malária/etiologia
10.
J Org Chem ; 80(16): 7849-55, 2015 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-26222145

RESUMO

Bastimolide A (1), a polyhydroxy macrolide with a 40-membered ring, was isolated from a new genus of the tropical marine cyanobacterium Okeania hirsuta. This novel macrolide was defined by spectroscopy and chemical reactions to possess one 1,3-diol, one 1,3,5-triol, six 1,5-diols, and one tert-butyl group; however, the relationships of these moieties to one another were obscured by a highly degenerate (1)H NMR spectrum. Its complete structure and absolute configuration were therefore unambiguously determined by X-ray diffraction analysis of the nona-p-nitrobenzoate derivative (1d). Pure bastimolide A (1) showed potent antimalarial activity against four resistant strains of Plasmodium falciparum with IC50 values between 80 and 270 nM, although with some toxicity to the control Vero cells (IC50 = 2.1 µM), and thus represents a potentially promising lead for antimalarial drug discovery. Moreover, rigorous establishment of its molecular arrangement gives fresh insight into the structures and biosynthesis of cyanobacterial polyhydroxymacrolides.


Assuntos
Antimaláricos/farmacologia , Cianobactérias/química , Macrolídeos/farmacologia , Plasmodium falciparum/efeitos dos fármacos , Antimaláricos/química , Antimaláricos/isolamento & purificação , Resistência a Medicamentos , Macrolídeos/química , Macrolídeos/isolamento & purificação , Conformação Molecular , Testes de Sensibilidade Parasitária , Relação Estrutura-Atividade
11.
Malar J ; 13: 18, 2014 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-24410874

RESUMO

BACKGROUND: Plinia cerrocampanensis is an endemic plant of Panama. The leaf essential oil of this plant has shown antibacterial activity. However, anti-malarial activity and chemical profiling by HS-SPME-GC-MS of this essential oil have not been reported before. METHODS: Anti-malarial activity of the essential oil (EO) was evaluated in vitro against chloroquine-sensitive HB3 and chloroquine-resistant W2 strains of Plasmodium falciparum. Synergistic effect of chloroquine and the EO on parasite growth was evaluated by calculating the combination index. A methodology involving headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) was developed to investigate the composition of Plinia cerrocampanensis EO. RESULTS: Plinia cerrocampanensis EO showed a high anti-malarial activity and a synergistic interaction with chloroquine. The Plinia cerrocampanensis EO inhibited P. falciparum growth in vitro at an IC50 of 7.3 µg/mL. Chloroquine together with the EO decreased the IC50 of chloroquine from 0.1 µg/mL to 0.05 µg/mL, and of the EO from 7.3 µg/mL to 1.1 µg/mL. The measured combination index was 0.58, which clearly indicates that the EO acts synergistically with chloroquine. Since the EO maintained its inhibitory activity on the chloroquine-sensitive strain of the parasite, it could be acting by a different mechanism of action than chloroquine. The best HS-SPME-GC-MS analytical conditions were obtained when the temperature of extraction was 49°C, incubation time 14 min, and the time of extraction 10 min. This method allowed for the identification of 53 volatile constituents in the EO, including new compounds not reported earlier. CONCLUSIONS: The anti-malarial activity exhibited by the Plinia cerrocampanensis EO may lend support for its possible use as an alternative for anti-malarial therapy.


Assuntos
Antimaláricos/farmacologia , Cloroquina/farmacologia , Cromatografia Gasosa-Espectrometria de Massas/métodos , Myrtaceae/química , Óleos Voláteis/farmacologia , Folhas de Planta/química , Plasmodium falciparum/efeitos dos fármacos , Microextração em Fase Sólida/métodos , Antimaláricos/análise , Cloroquina/análise , Óleos Voláteis/química , Panamá
12.
HardwareX ; 19: e00540, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38988372

RESUMO

Recently, a novel method for the growth inhibition of malaria parasites using microwaves was proposed. However, the apparatuses used to demonstrate this method are high-cost and immovable, hindering the progression in this field of research, which is still in its early stages. This paper presents the redesign, construction, and validation of an equivalent system, converting it into a portable and low-cost system, capable of replacing the existing one. The proposed system is mainly composed of an RF generator (MAX2870), an RF amplifier (SKYWORKS 66292-11) and a graphical user interface. Likewise, the RF applicator proposed by the original study was redesigned, resulting in a five-fold improvement in return loss. The obtained results indicate that the proposed system achieves 90% parasite growth inhibition, matching the performance of its counterpart at less than 1% of its cost. These results represent a breakthrough for the creation of smaller, enhanced devices that open new possibilities for an alternative treatment to combat this devastating disease.

13.
Cells ; 13(4)2024 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-38391947

RESUMO

Plasmodium parasites need to find red blood cells (RBCs) that, on the one hand, expose receptors for the pathogen ligands and, on the other hand, maintain the right geometry to facilitate merozoite attachment and entry into the red blood cell. Both characteristics change with the maturation of erythrocytes. Some Plasmodia prefer younger vs. older erythrocytes. How does the life evolution of the RBC affect the invasion of the parasite? What happens when the RBC ages? In this review, we present what is known up until now.


Assuntos
Malária Falciparum , Plasmodium falciparum , Humanos , Envelhecimento Eritrocítico , Malária Falciparum/parasitologia , Eritrócitos/parasitologia , Proteínas de Transporte
14.
Micromachines (Basel) ; 15(5)2024 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-38793163

RESUMO

Pathological processes often change the mechanical properties of cells. Increased rigidity could be a marker of cellular malfunction. Erythrocytes are a type of cell that deforms to squeeze through tiny capillaries; changes in their rigidity can dramatically affect their functionality. Furthermore, differences in the homeostatic elasticity of the cell can be used as a tool for diagnosis and even for choosing the adequate treatment for some illnesses. More accurate types of equipment needed to study biomechanical phenomena at the single-cell level are very costly and thus out of reach for many laboratories around the world. This study presents a simple and low-cost technique to study the rigidity of red blood cells (RBCs) through the application of electric fields in a hand-made microfluidic chamber that uses a capacitor principle. As RBCs are deformed with the application of voltage, cells are observed under a light microscope. From mechanical force vs. deformation data, the elastic constant of the cells is determined. The results obtained with the capacitor-based method were compared with those obtained using optical tweezers, finding good agreement. In addition, P. falciparum-infected erythrocytes were tested with the electric field applicator. Our technique provides a simple means of testing the mechanical properties of individual cells.

15.
Sci Rep ; 13(1): 285, 2023 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-36609676

RESUMO

Malaria cases and deaths keep being excessively high every year. Some inroads gained in the last two decades have been eroded especially due to the surge of resistance to most antimalarials. The search for new molecules that can replace the ones currently in use cannot stop. In this report, the synthesis of benzimidazole derivatives guided by structure-activity parameters is presented. Thirty-six molecules obtained are analyzed according to their activity against P. falciparum HB3 strain based on the type of substituent on rings A and B, their electron donor/withdrawing, as well as their dimension/spatial properties. There is a preference for electron donating groups on ring A, such as Me in position 5, or better, 5, 6-diMe. Ring B must be of the pyridine type such as picolinamide, other modifications are generally not favorable. Two molecules, 1 and 33 displayed antiplasmodial activity in the high nanomolar range against the chloroquine sensitive strain, with selectivity indexes above 10. Activity results of 1, 12 and 16 on a chloroquine resistance strain indicated an activity close to chloroquine for compound 1. Analysis of some of their effect on the parasites seem to suggest that 1 and 33 affect only the parasite and use a route other than interference with hemozoin biocrystallization, the route used by chloroquine and most antimalarials.


Assuntos
Antimaláricos , Malária Falciparum , Humanos , Antimaláricos/química , Plasmodium falciparum , Cloroquina/uso terapêutico , Relação Estrutura-Atividade , Malária Falciparum/parasitologia , Benzimidazóis/farmacologia , Benzimidazóis/uso terapêutico
16.
J Vis Exp ; (194)2023 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-37154542

RESUMO

Protozoan parasites of the genus Leishmania cause leishmaniasis, a disease with variable clinical manifestations that affects millions of people worldwide. Infection with L. donovani can result in fatal visceral disease. In Panama, Colombia, and Costa Rica, L. panamensis is responsible for most of the reported cases of cutaneous and mucocutaneous leishmaniasis. Studying a large number of drug candidates with the methodologies available to date is quite difficult, given that they are very laborious for evaluating the activity of compounds against intracellular forms of the parasite or for performing in vivo assays. In this work, we describe the generation of L. panamensis and L. donovani strains with constitutive expression of the gene that encodes for an enhanced green fluorescent protein (eGFP) integrated into the locus that encodes for 18S rRNA (ssu). The gene encoding eGFP was obtained from a commercial vector and amplified by polymerase chain reaction (PCR) to enrich it and add restriction sites for the BglII and KpnI enzymes. The eGFP amplicon was isolated by agarose gel purification, digested with the enzymes BglII and KpnI, and ligated into the Leishmania expression vector pLEXSY-sat2.1 previously digested with the same set of enzymes. The expression vector with the cloned gene was propagated in E. coli, purified, and the presence of the insert was verified by colony PCR. The purified plasmid was linearized and used to transfect L. donovani and L. panamensis parasites. The integration of the gene was verified by PCR. The expression of the eGFP gene was evaluated by flow cytometry. Fluorescent parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity were selected using flow cytometry.


Assuntos
Leishmania donovani , Leishmania , Leishmaniose , Humanos , Escherichia coli , Leishmania/genética , Leishmaniose/parasitologia , Proteínas de Fluorescência Verde/genética , Leishmania donovani/genética
17.
Front Cell Infect Microbiol ; 13: 955134, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36816585

RESUMO

Malaria, which infected more than 240 million people and killed around six hundred thousand only in 2021, has reclaimed territory after the SARS-CoV-2 pandemic. Together with parasite resistance and a not-yet-optimal vaccine, the need for new approaches has become critical. While earlier, limited, studies have suggested that malaria parasites are affected by electromagnetic energy, the outcomes of this affectation vary and there has not been a study that looks into the mechanism of action behind these responses. In this study, through development and implementation of custom applicators for in vitro experimentation, conditions were generated in which microwave energy (MW) killed more than 90% of the parasites, not by a thermal effect but via a MW energy-induced programmed cell death that does not seem to affect mammalian cell lines. Transmission electron microscopy points to the involvement of the haemozoin-containing food vacuole, which becomes destroyed; while several other experimental approaches demonstrate the involvement of calcium signaling pathways in the resulting effects of exposure to MW. Furthermore, parasites were protected from the effects of MW by calcium channel blockers calmodulin and phosphoinositol. The findings presented here offer a molecular insight into the elusive interactions of oscillating electromagnetic fields with P. falciparum, prove that they are not related to temperature, and present an alternative technology to combat this devastating disease.


Assuntos
COVID-19 , Malária Falciparum , Malária , Parasitos , Animais , Humanos , Micro-Ondas , SARS-CoV-2 , Malária Falciparum/parasitologia , Plasmodium falciparum , Mamíferos
18.
PLoS Pathog ; 6(6): e1000968, 2010 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-20585558

RESUMO

Plasmodium falciparum is a highly lethal malaria parasite of humans. A major portion of its life cycle is dedicated to invading and multiplying inside erythrocytes. The molecular mechanisms of erythrocyte invasion are incompletely understood. P. falciparum depends heavily on sialic acid present on glycophorins to invade erythrocytes. However, a significant proportion of laboratory and field isolates are also able to invade erythrocytes in a sialic acid-independent manner. The identity of the erythrocyte sialic acid-independent receptor has been a mystery for decades. We report here that the complement receptor 1 (CR1) is a sialic acid-independent receptor for the invasion of erythrocytes by P. falciparum. We show that soluble CR1 (sCR1) as well as polyclonal and monoclonal antibodies against CR1 inhibit sialic acid-independent invasion in a variety of laboratory strains and wild isolates, and that merozoites interact directly with CR1 on the erythrocyte surface and with sCR1-coated microspheres. Also, the invasion of neuraminidase-treated erythrocytes correlates with the level of CR1 expression. Finally, both sialic acid-independent and dependent strains invade CR1 transgenic mouse erythrocytes preferentially over wild-type erythrocytes but invasion by the latter is more sensitive to neuraminidase. These results suggest that both sialic acid-dependent and independent strains interact with CR1 in the normal red cell during the invasion process. However, only sialic acid-independent strains can do so without the presence of glycophorin sialic acid. Our results close a longstanding and important gap in the understanding of the mechanism of erythrocyte invasion by P. falciparum that will eventually make possible the development of an effective blood stage vaccine.


Assuntos
Eritrócitos/metabolismo , Eritrócitos/parasitologia , Malária Falciparum/metabolismo , Glicoproteínas de Membrana/metabolismo , Ácido N-Acetilneuramínico/metabolismo , Plasmodium falciparum/fisiologia , Receptores de Complemento/metabolismo , Receptores Imunológicos/metabolismo , Animais , Western Blotting , Citometria de Fluxo , Imunofluorescência , Humanos , Imunoprecipitação , Malária Falciparum/virologia , Merozoítos/fisiologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Lectina 1 Semelhante a Ig de Ligação ao Ácido Siálico
19.
J Nat Prod ; 75(1): 60-6, 2012 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-22148360

RESUMO

Credneramides A (1) and B (2), two vinyl chloride-containing metabolites, were isolated from a Papua New Guinea collection of cf. Trichodesmium sp. nov. and expand a recently described class of vinyl chloride-containing natural products. The precursor fatty acid, credneric acid (3), was isolated from both the aqueous and organic fractions of the parent fraction as well as from another geographically and phylogenetically distinct cyanobacterial collection (Panama). Credneramides A and B inhibited spontaneous calcium oscillations in murine cerebrocortical neurons at low micromolar concentrations (1, IC(50) 4.0 µM; 2, IC(50) 3.8 µM).


Assuntos
Aminas/isolamento & purificação , Aminas/farmacologia , Ácidos Graxos/química , Ácidos Graxos/farmacologia , Neurotransmissores/isolamento & purificação , Neurotransmissores/farmacologia , Fenetilaminas/farmacologia , Aminas/química , Animais , Sequência de Bases , Cianobactérias , Ácidos Graxos/isolamento & purificação , Camundongos , Estrutura Molecular , Neurotransmissores/química , Papua Nova Guiné , Fenetilaminas/química , Fenetilaminas/isolamento & purificação , Percepção de Quorum/fisiologia
20.
Tetrahedron Lett ; 53(8): 919-922, 2012 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-25177062

RESUMO

Three new terpenoids of mixed biosynthetic origin were isolated from the culture filtrate of the endophytic fungus Pycnoporus sanguineus. Their structures were determined by extensive spectroscopic analyses. We have named these tricyclic and tetracyclic metabolites 'coibanoles A-C' in reference to Coiba Island and Coiba National Park, Panamá, from which the plant and endophyte were collected. The extract was inactive to the human parasites Trypanosoma cruzi, Leishmania donovani, and Plasmodium falciparum at a test concentration of 10 µg/mL.

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