An mRNA methylase and demethylase regulate sorghum salt tolerance by mediating N6-methyladenosine modification.

N 6-methyladenosine (m6A) modification is a crucial and widespread molecular mechanism governing plant development and stress tolerance. The specific impact of m6A regulation on plants with inherently high salt tolerance remains unclear. Existing research primarily focuses on the overexpression or knockout of individual writer or eraser components to alter m6A levels. However, a comprehensive study simultaneously altering overall m6A modification levels within the same experiment is lacking. Such an investigation is essential to determine whether opposing changes in m6A modification levels exert entirely different effects on plant salt tolerance. In this study, we identified the major writer member mRNA adenosine methylase A (SbMTA) in sorghum (Sorghum bicolor) as critical for sorghum survival. The sbmta mutant exhibits a phenotype characterized by reduced overall m6A, developmental arrest, and, ultimately, lethality. Overexpression of SbMTA increased m6A levels and salt tolerance, while overexpression of the m6A eraser alkylated DNA repair protein AlkB homolog 10B (SbALKBH10B) in sorghum showed the opposite phenotype. Comparative analyses between sorghum with different m6A levels reveal that SbMTA- and SbALKBH10B-mediated m6A alterations significantly impact the stability and expression levels of genes related to the ABA signaling pathway and growth under salt stress. In summary, this study unveils the intricate relationship between m6A modifications and salt tolerance in sorghum, providing valuable insights into how m6A modification levels on specific transcripts influence responses to salt stress.

MicroRNAs balance growth and salt stress responses in sweet sorghum.

Salt stress is one of the major causes of reduced crop production, limiting agricultural development globally. Plants have evolved with complex systems to maintain the balance between growth and stress responses, where signaling pathways such as hormone signaling play key roles. Recent studies revealed that hormones are modulated by microRNAs (miRNAs). Previously, two sweet sorghum (Sorghum bicolor) inbred lines with different salt tolerance were identified: the salt-tolerant M-81E and the salt-sensitive Roma. The levels of endogenous hormones in M-81E and Roma varied differently under salt stress, showing a different balance between growth and stress responses. miRNA and degradome sequencing showed that the expression of many upstream transcription factors regulating signal transduction and hormone-responsive genes was directly induced by differentially expressed miRNAs, whose levels were very different between the two sweet sorghum lines. Furthermore, the effects of representative miRNAs on salt tolerance in sorghum were verified through a transformation system mediated by Agrobacterium rhizogenes. Also, miR-6225-5p reduced the level of Ca2+ in the miR-6225-5p-overexpressing line by inhibiting the expression of the Ca2+ uptake gene SbGLR3.1 in the root epidermis and affected salt tolerance in sorghum. This study provides evidence for miRNA-mediated growth and stress responses in sweet sorghum.

The treatment of phosphogypsum leachate is more urgent than phosphogypsum.

Phosphogypsum(PG) is one of the typical bulk industrial solid wastes generated in the phosphate chemical industry. Due to its huge production volume and immature resource treatment technology, a large amount of PG can only be stored and disposed in slag yards, and its impact on the ecological environment is becoming increasingly significant during long-term storage. Up to now, many researchers have focused their research on PG, with less attention paid to the PG leachate(PG-L). On the basis of the resource utilization of PG, this article analyzed the migration and transformation of pollutants and their impact on the ecological environment during long-term storage of PG. The content of pollutants in PG-L and PG was compared, and it was found that the content of toxic and harmful substances in PG-L was significantly higher than that in PG itself, and the pollution diffusion ability was greater than that of PG, the pollution of PG to the ecological environment is mainly caused by PG-L, indicating that the harmless treatment of PG-L is more urgent than PG. On the basis of traditional leachate treatment methods, a new technology of valuable element recovery and electrochemical synergistic treatment is proposed to achieve high value-added treatment of PG-L.

Halotolerant Bacillus sp. strain RA coordinates myo-inositol metabolism to confer salt tolerance to tomato.

Soil salinity is a worldwide problem threatening crop yields. Some plant growth-promoting rhizobacteria (PGPR) could survive in high salt environment and assist plant adaptation to stress. Nevertheless, the genomic and metabolic features, as well as the regulatory mechanisms promoting salt tolerance in plants by these bacteria remain largely unknown. In the current work, a novel halotolerant PGPR strain, namely, Bacillus sp. strain RA can enhance tomato tolerance to salt stress. Comparative genomic analysis of strain RA with its closely related species indicated a high level of evolutionary plasticity exhibited by strain-specific genes and evolutionary constraints driven by purifying selection, which facilitated its genomic adaptation to salt-affected soils. The transcriptome further showed that strain RA could tolerate salt stress by balancing energy metabolism via the reprogramming of biosynthetic pathways. Plants exude a plethora of metabolites that can strongly influence plant fitness. The accumulation of myo-inositol in leaves under salt stress was observed, leading to the promotion of plant growth triggered by Bacillus sp. strain RA. Importantly, myo-inositol serves as a selective force in the assembly of the phyllosphere microbiome and the recruitment of plant-beneficial species. It promotes destabilizing properties in phyllosphere bacterial co-occurrence networks, but not in fungal networks. Furthermore, interdomain interactions between bacteria and fungi were strengthened by myo-inositol in response to salt stress. This work highlights the genetic adaptation of RA to salt-affected soils and its ability to impact phyllosphere microorganisms through the adjustment of myo-inositol metabolites, thereby imparting enduring resistance against salt stress in tomato.

R2R3 MYB transcription factor SbMYBHv33 negatively regulates sorghum biomass accumulation and salt tolerance.

KEY MESSAGE: SbMYBHv33 negatively regulated biomass accumulation and salt tolerance in sorghum and Arabidopsis by regulating reactive oxygen species accumulation and ion levels. Salt stress is one of the main types of environmental stress leading to a reduction in crop yield worldwide. Plants have also evolved a variety of corresponding regulatory pathways to resist environmental stress damage. This study aimed to identify a SbMYBHv33 transcription factor that downregulates in salt, drought, and abscisic acid (ABA) in the salt-tolerant inbred line sorghum M-81E. The findings revealed that overexpression of SbMYBHv33 in sorghum significantly reduced sorghum biomass accumulation at the seedling stage and also salinity tolerance. Meanwhile, a heterologous transformation of Arabidopsis with SbMYBHv33 produced a similar phenotype. The loss of function of the Arabidopsis homolog of SbMYBHv33 resulted in longer roots and increased salt tolerance. Under normal conditions, SbMYBHV33 overexpression promoted the expression of ABA pathway genes in sorghum and inhibited growth. Under salt stress conditions, the gene expression of SbMYBHV33 decreased in the overexpressed lines, and the promotion of these genes in the ABA pathway was attenuated. This might be an important reason for the difference in growth and stress resistance between SbMYBHv33-overexpressed sorghum and ectopic expression Arabidopsis. Hence, SbMYBHv33 is an important component of sorghum growth and development and the regulation of salt stress response, and it could negatively regulate salt tolerance and biomass accumulation in sorghum.

Harmless and resourceful utilization of solid waste: Multi physical field regulation in the microbiological treatment process of solid waste treatment.

Solid waste (SW) treatment methods mainly include physical, chemical, and biological methods, while physical and chemical methods have advantages such as fast effectiveness and short treatment time, but have high costs and were prone to secondary pollution. Due to the advantages of mild conditions and environmental protection, microbial methods have attracted the attention of numerous researchers. Recently, promotion of biological metabolic activity in biotreatment technology by applying multiple physical conditions, and reducing the biochemical reaction energy base to promote the transfer of protons and electrons, has made significant progress in harmless and resourceful utilization of SW. This paper main summarized the harmless and resourceful treatment methods of common bulk SW. The research of physical field-enhanced microbial treatment of inorganic solid waste (ISW) and organic solid waste (OSW) was discussed. The advantages and mechanisms of microbial treatment compared to traditional SW treatment methods were analyzed. The multi-physical field coupling enhanced microbial treatment technology was proposed to further improving the efficiency of large-scale treatment of bulk SW. The application prospects and potential opportunities of this technology were analyzed. Novel research ideas for the large-scale harmless and resourceful treatment of bulk SW were provided.

Soilization utilization of solid waste: Ecological regulation of phosphorus tailings-based soil with physicochemical improvement and Bacillus_cereus-addition.

Extraction and utilization of effective phosphorus from solid waste have been an important approach for alleviating phosphorus resource shortage. The extraction of available phosphorus by microbial method with low cost, mild conditions and simple process has been drawing attention from the majority of research scholars. However, relevant studies on special microbial communities for effective phosphorus extraction from solid waste are less. In this work，a functional Bacillus_cereus strain screened from phosphate tailings, phosphate ore and forest rhizosphere soil was inoculated into phosphate tailings (PT), modified phosphate tailings (IS) and highland red soil (SS). Compared with SS, the water-holding properties, fertility, leaching toxicity and microbial community diversity of PT and IS with and without bacteria were analyzed. PT+, SS+ and IS+ (after adding bacteria to PT, SS and IS) showed moderately alkaline pH, and the available phosphorus content enhanced by 31.73%, 20.05% and 39.41% respectively. The leaching toxicity phosphate of PT+ and IS + decreased by 4.89 mg/kg and 2.61 mg/kg respectively, while that of SS + increased by 5.45 mg/kg, indicating differences in the phosphorus solubilization mechanism of Bacillus_cereus for different soils. Furthermore, the modification and bacteria treatment improved the relative abundance of Pedobacter, Alcaligenaceae and Pseudomonas, thus enhancing the phosphorus solubility of the PT bacterial community. This work may achieve efficient utilization and ecological restoration of phosphorus tailings-based soil and contribute to long-term sustainable agricultural development.

Recycling and reutilization of smelting dust as a secondary resource: A review.

Smelting dust is a toxic waste produced in metal-mineral pyrometallurgical processes. To eliminate or reduce the adverse environmental impacts of smelting dust, valuable components need to be selectively separated from the toxic components present in the waste. This paper reviews the chemical composition, phase composition and particle size distribution characteristics of smelting dust, and the results show that smelting dust has excellent physicochemical characteristics for recovering valuable metals. The process flow, critical factors, development status, advantages and disadvantages of traditional technologies such as pyrometallurgy, hydrometallurgy and biometallurgy were discussed in depth. Conventional treatment methods typically prioritize separating and reclaiming specific elements with high concentrations. However, these methods face challenges such as excessive chemical usage and limited selectivity, which can hinder the sustainable utilization of smelting dust. With the increasing scarcity of resources and strict environmental requirements, a single treatment process can hardly fulfil the demand, and a physical field-enhanced technology for releasing and separating valuable metals is proposed. Through analysing the effect of electric field, microwave and ultrasound on recovering valuable metals from smelting dust, the enhancement mechanism of physical field on the extraction process was clarified. This paper aimed to provide reference for the resource utilization of smelting dust.

Aluminium sulfate synergistic electrokinetic separation of soluble components from phosphorus slag and simultaneous stabilization of fluoride.

In this study, fluoride (F) was stabilized and soluble components, namely phosphate (P), K, Ca, Cr, Mn, and Pb, were extracted from phosphorus slag (PS) by using aluminum sulfate (AS) synergistic electrokinetic. PHREEQC simulation was used to determine the occurrence form of each ion in the PS. The mechanisms by which various electrokinetic treatment methods affected conductivity and pH distribution were carefully investigated. Electrokinetic treatment increased P concentration of the anode chamber from 22.7 mg/L to 63.39 mg/L, whereas K concentration increased from 15.26 mg/L to 93.44 mg/L. After AS-enhanced electrokinetic treatments, the concentrations of the different components were as follows: P, 131.66 mg/L; K, 198.2 mg/L; and Ca, 331.3 mg/L. The removal rate of soluble P in PS slices increased to 80.88% by 1.5 V/cm of treatment, and it increased to 94.04% after AS enhancement treatment. For water-soluble F, the removal rate from the PS slices in the anode region was 86.03%, decreasing F concentration in the electrode chamber to 9.57 × 10-3 mg/L. Different extraction efficiencies and stability levels of each component in the PS were regulated at various electrode regions by using different processes such as electromigration, electro-osmotic flow, flocculation, and precipitation. Good results can be obtained if fluoride is solidified concurrently with the removal or recovery of P, K, Ca, and other elements using 2%-4% AS enhanced electrokinetic treatment. Furthermore, CaSO4·2H2O whiskers were produced in the electrode regions when AS content was 6%. The findings of this study indicated that the AS synergistic electrokinetic method is suitable for stabilizing F and removing heavy metals from PS, thus providing a promising technology for recycling valuable components such as P, K, Ca, and Sr and for the simultaneous production of CaSO4·2H2O whiskers. This study provides insights for developing novel technologies for the clean treatment and high-value utilization of PS.

SbWRKY55 regulates sorghum response to saline environment by its dual role in abscisic acid signaling.

KEY MESSAGE: SbWRKY55 functions as a key component of the ABA-mediated signaling pathway; transgenic sorghum regulates plant responses to saline environments and will help save arable land and ensure food security. Salt tolerance in plants is triggered by various environmental stress factors and endogenous hormonal signals. Numerous studies have shown that WRKY transcription factors are involved in regulating plant salt tolerance. However, the underlying mechanism for WRKY transcription factors regulated salt stress response and signal transduction pathways remains largely unknown. In this study, the SbWRKY55 transcription factor was found to be the key component for reduced levels of salt and abscisic acid in SbWRKY55 overexpression significantly reduced salt tolerance in sorghum and Arabidopsis. Mutation of the homologous gene AtWRKY55 in A. thaliana significantly enhanced salt tolerance, and SbWRKY55 supplementation in the mutants restored salt tolerance. In the transgenic sorghum with SbWRKY55 overexpression, the expression levels of genes involved in the abscisic acid (ABA) pathway were altered, and the endogenous ABA content decreased. Yeast one-hybrid assays and dual-luciferase reporter assay showed that SbWRKY55 binds directly to the promoter of SbBGLU22 and inhibits its expression level. In addition, both in vivo and in vitro biochemical analyses showed that SbWRKY55 interacts with the FYVE zinc finger protein SbFYVE1, blocking the ABA signaling pathway. This could be an important feedback regulatory pathway to balance the SbWRKY55-mediated salt stress response. In summary, the results of this study provide convincing evidence that SbWRKY55 functions as a key component in the ABA-mediated signaling pathway, highlighting the dual role of SbWRKY55 in ABA signaling. This study also showed that SbWRKY55 could negatively regulate salt tolerance in sorghum.

Identification and Transcriptome Analysis of Genes Related to Membrane Lipid Regulation in Sweet Sorghum under Salt Stress.

Sweet sorghum has strong stress resistance and is considered a promising energy crop. In the present study, the effects of salt on the membrane lipid metabolism of two sweet sorghum inbred lines (salt-tolerant M-81E and salt-sensitive Roma) were analyzed. After treatment with 150 mM NaCl, higher levels of fresh weight and chlorophyll fluorescence, as well as lower levels of malondialdehyde (MDA) were found in salt-tolerant M-81E. Concomitantly, 702 and 1339 differentially expression genes (DEGs) in M-81E and Roma were identified in response to salt stress. We determined that most DEGs were related to glycerophospholipid metabolism, glycerolipid metabolism, and other membrane lipid metabolisms. Under NaCl treatment, the expression of the membrane-associated phospholipase A1 was down-regulated at the transcriptional level, along with an increased content of phosphatidylcholine (PC) in both cultivars. The inhibition of triacylglycerol (TAG) mobilization in M-81E delayed salt-induced leaf senescence. Furthermore, enhanced levels of glycerol-3-phosphate acyltransferase (GPAT) expression contributed to improved salt resistance in M-81E. The results of this study demonstrate membrane the role of lipid regulation in mediating salt-defensive responses in sweet sorghum and expand our understanding of the relationship between changes in membrane lipid content and salt resistance.

Harmless treatment technology of phosphogypsum: Directional stabilization of toxic and harmful substances.

Phosphogypsum is one of the typical by-products of phosphorus chemical industry. As a strategic industry related to the national livelihood of China, phosphorus chemical industry has accumulated and produced a significant amount of phosphogypsum. In general, phosphogypsum contains approximately 80%-95% calcium sulfate dihydrate, and less than 5% toxic and harmful elements. In this paper, toxic and hazardous components in phosphogypsum were efficiently solidified and stabilized by highly targeted solidification and stabilization technology. Calcium carbide slag or lime was used as an alkali-base neutralizer of phosphogypsum, and polymeric ferric sulfate or polymeric aluminum chloride as a directional solidification stabilizer to analyze the leaching toxicity of the mixed powder in 1, 3, 5 and 15 days. The experimental results demonstrate excellent solidification and stabilization effect with the leaching pH of 6-9, the leaching concentration of P, F and heavy metals of less than 0.5 mg/L, 10 mg/L and 0.1 mg/L, respectively, which meets the requirements of relevant international standards. Mechanistic analysis indicates that the solidification and stabilization of toxic and hazardous substances in phosphogypsum is perfectly achieved owing to the generation, adsorption and encapsulation of insoluble substances. This technology can reduce the costs and difficulty in the phosphogypsum treatment, and has extensive application potentials.

Utilization path of bulk industrial solid waste: A review on the multi-directional resource utilization path of phosphogypsum.

Phosphogypsum is one of the hottest issues in the field of environmental solid waste treatment, with complex and changeable composition. Meanwhile, phosphogypsum contains a large number of impurities, thus leading to the low resource utilization rate, and it can only be stockpiled in large quantities. Phosphogypsum occupies a lot of land and poses a serious pollution threat to the ecological environment. This paper mainly summarizes the existing pretreatment and resource utilization technology of phosphogypsum. The pretreatment mainly includes dry method and wet method. The resource utilization technology mainly includes building materials, chemical raw materials, agriculture, environmental functional materials, filling materials, carbon sequestration and rare and precious extraction. Although there are many aspects of resource utilization of phosphogypsum, the existing technology is far from being able to consume a large amount of accumulated and generated phosphogypsum. Through the analysis, the comparison and mechanism analysis of the existing multifaceted and multi-level resource treatment technologies of phosphogypsum, the four promising resource utilization directions of phosphogypsum are put forward, mainly including prefabricated building materials, eco-friendly materials and soil materials, and new green functional materials and chemical fillers. Moreover, this paper summarizes the research basis of multi field and all-round treatment and disposal of phosphogypsum, which reduces repeated researches and development, as well as the treatment cost of phosphogypsum. This paper could provide a feasible research direction for the resource treatment technology of phosphogypsum in the future, so as to improve the consumption of phosphogypsum and reduce environmental risks.

Study of Semi-Dry High Target Solidification/Stabilization of Harmful Impurities in Phosphogypsum by Modification.

Phosphogypsum (PG) treatment is one of the research hotspots in the field of environmental protection. Many researchers both at home and abroad have devoted themselves to studies on harmless resource treatment of PG, but the treatment technology is unable to meet the demand of PG consumption due to the huge production and storage demands. In order to solve the problem of PG pollution, this study explored the different solidified effects of various modification formulations on the hazardous components in PG, using industrial solid waste calcium carbide slag (CCS) as an alkaline regulator; Portland cement (PC), polyaluminum chloride (PAC) and CaCl2 as the main raw materials of the solidification and stabilization formula and the water content in PG as the reaction medium. The results showed that CCS (0.5%), PC (0.4%) and PAC (0.3%) had a more significant solidified effect on phosphorus (P) and fluoride (F). PAC was added in two steps and reacted under normal temperature and pressure, and its leaching toxicity meets the requirements of relevant standards, which laid an excellent foundation for PG-based ecological restoration materials and filling materials, with low economic cost, simple process and strong feasibility. This will provide great convenience for the later mining and metallurgy.

Advances in the research of carbon-, silicon-, and polymer-based superhydrophobic nanomaterials: Synthesis and potential application.

With the rapid development of science and technology, superhydrophobic nanomaterials have become one of the hot topics from various subjects. Due to their distinct properties, such as superhydrophobicity, anti-icing and corrosion resistance, superhydrophobic nanomaterials are widely used in industry, agriculture, defense, medicine and other fields. Hence, the development of superhydrophobic materials with superior performance, economical, practical features, and environment-friendly properties are extremely important for industrial development and environmental protection. Aimed to provide a scientific and theoretical basis for the subsequent study on the preparation of composite superhydrophobic nanomaterials, this paper reviewed the latest progress in the research of superhydrophobic surface wettability and the theory of superhydrophobicity, summarized and analyzed the latest development of carbon-based, silicon-based and polymer-based superhydrophobic nanomaterials in terms of their synthesis, modification, properties and structure sizes (diameters), discussed the problems and unique application prospects of carbon-based, silicon-based and polymer-based superhydrophobic nanomaterials.

Nano Biomass Material functionalized by ß-CD@Ce(NO)3 as a high performance adsorbent to removal of fluorine from wastewater.

Fluorine pollution has become one of the key issues of water pollution, and the adsorption materials for efficient removal of fluorine ions have attracted much attention. It is rarely reported that the self-synthesized biomass materials were functionalized by the ß-CD@Ce(NO)3. This paper mainly proposed a new synthetic method of the self-synthesized biomass materials were modified by the ß-CD@Ce(NO)3 and removal of fluorine ions. The effects of this materials on the adsorption efficiency of fluorine ions under different conditions were explored, and the kinetic and thermodynamic simulations were carried out. The results show that the self-synthesized biomass materials were modified by the ß-CD@Ce(NO)3 has significant pore structure, large specific surface area and multi-functional group. Adsorption experiment showed that the reaction reached adsorption equilibrium at 30 min. The removal rate of fluorine ions reached 93.13%, and the fluorine ions adsorption capacity was 37.25 mg/g under neutral conditions. The material can be recycled for more than 5 times, and the adsorption efficiency remains above 94%. The adsorption kinetics accorded with the pseudo second-order model and the adsorption isotherm equation is in line with the Langmuir isotherm adsorption model. PO43- and CO32- have the most impact on fluorine ions adsorption. This method reduces the synthesis cost of high-performance adsorption materials and improves the adsorption performance, which is conducive to the popularization and application in the future.

Study on the mechanism of rapid degradation of Rhodamine B with Fe/Cu@antimony tailing nano catalytic particle electrode in a three dimensional electrochemical reactor.

A novel particle electrode based on antimony tailings microspheres was successfully constructed by ultrasonic immersion calcination method, and the degradation of RhB was studied in a three-dimensional electrochemical reactor (3DER). It was characterized by XRD, SEM, EDS, XPS, cyclic voltammetry and linear sweep voltammetry. When the pH value is 5.00, the dosage of Fe/Cu@antimony tailing is 1.50 g/L, the initial concentration is 100 mg/L, and the current density is 20 mA/cm2, the degradation efficiency is the best (99.40% for RhB and 98.81% for TOC) within 15 min. The results show that in the three-dimensional electrochemical oxidation system, electrochemical oxidation and electro Fenton oxidation occur at the same time to cause the increase of hydroxyl radicals. According to LC-MS analysis and EPR characterization, it can be found that the main degradation mechanism of RhB is that hydroxyl radicals continuously attack RhB, and realize rapid degradation of RhB through deethylation, deamination, dealkylation, decarboxylation, chromophore splitting, ring opening and mineralization. Fe/Cu@antimony tailing particles are both electrodes for electrochemical oxidation and catalysts for Fenton oxidation. The degradation effect of RhB remained at 94% after 6 cycles, and the leaching rates of Fe and Cu are only 1.20% and 0.79%, indicating that Fe/Cu@AT had significant stability. This work provides a new insight into the establishment of an efficient and stable three-dimensional electrocatalytic particle electrode.

Biological Functions of Strigolactones and Their Crosstalk With Other Phytohormones.

Phytohormones are small chemicals critical for plant development and adaptation to a changing environment. Strigolactones (SLs), carotenoid-derived small signalling molecules and a class of phytohormones, regulate multiple developmental processes and respond to diverse environmental signals. SLs also coordinate adjustments in the balance of resource distribution by strategic modification of the plant development, allowing plants to adapt to nutrient deficiency. Instead of operating independently, SL interplays with abscisic acid, cytokinin, auxin, ethylene, and some other plant phytohormones, forming elaborate signalling networks. Hormone signalling crosstalk in plant development and environmental response may occur in a fully concerted manner or as a cascade of sequential events. In many cases, the exact underlying mechanism is unclear because of the different effects of phytohormones and the varying backgrounds of their actions. In this review, we systematically summarise the synthesis, signal transduction, and biological functions of SLs and further highlight the significance of crosstalk between SLs and other phytohormones during plant development and resistance to ever-changing environments.

Single-cell profiling lights different cell trajectories in plants.

The molecular mechanism of the maintenance and differentiation of plant stem cells is an eternal theme in studies on plant growth and development. Recent advances in single-cell RNA sequencing (scRNA-seq) methods have completely changed the understanding of cell heterogeneity and cell function, allowing research precision to identify the differentiation trajectory of stem cells maintained and differentiated at the cellular level. This review aimed to mainly discuss the novel insights provided by scRNA-seq for the maintenance and initiation of plant stem cells, cell differentiation, cell response to environmental changes, and improvement strategies for scRNA-seq. In addition, it highlighted additional perspectives beyond scRNA-seq, such as spatial transcriptomes, epigenomes, and single-cell multiomics, for a renewed understanding of stem cell maintenance and cell differentiation, thus providing potential targets and theoretical foundations for crop improvement.

Plant metabolomics integrated with transcriptomics and rhizospheric bacterial community indicates the mitigation effects of Klebsiella oxytoca P620 on p-hydroxybenzoic acid stress in cucumber.

Accumulation of p-hydroxybenzoic acid (PHBA) in soil causes autotoxicity stress in cucumber. When the stress is mitigated by PHBA-degrading bacteria, plant metabolites have not been detected. To explore mechanisms underlining the mitigation, plant metabolites have not been combined with rhizospheric microbes, antioxidant and soil enzymes. In this study, a strain P620 of Klebsiella decomposed PHBA to acetyl CoA. Cucumber was sown into soil supplemented with P620 and/or PHBA. After addition with P620, P620 colonization and the enriched bacterial genera were observed in rhizosphere. Compared to PHBA stress alone, the combination of P620 application and PHBA stress improved plant growth, decreased PHBA concentration in soil, and increased the activities of five soil enzymes and eight antioxidant enzymes in leaves. Metabolomic and transcriptomic analysis highlighted that P620 application decreased the intensities of MAG(18:3) isomer 4, MAG(18:3) isomer 2, lysoPC 18:3 (2n isomer), 2'-deoxyadenosine-5'-monophosphate, pyridoxine, and glucarate O-phosphoric acid in PHBA-stressed leaves and down-regulated the expression of genes related to these metabolites. We propose a mechanism that P620 application alters microbial communities in PHBA-contaminated soil. Thus, the application reduces PHBA concentration in soil, activates antioxidant and soil enzymes, and also influences metabolites in leaves by affecting plant transcriptome, mitigating PHBA stress in cucumber.