RESUMO
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
Assuntos
Proteínas de Transporte/metabolismo , Proteínas de Ligação a Ácido Graxo/metabolismo , Corantes Fluorescentes/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Células-Tronco Neurais/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Animais , Proliferação de Células , Proteína 7 de Ligação a Ácidos Graxos , Humanos , Camundongos , Células-Tronco Neurais/citologia , Ligação ProteicaRESUMO
"Aggregation-caused signal change" is a well-established mechanism by now and has been widely used as the basis for optical probe and sensor development. Compared to aggregation, its reverse process, disaggregation, has received much less attention and is not properly discussed in the literature so far. With the less established paradigm or mechanism, although some of the reported sensors and probes seem to work through disaggregation phenomena, the proper interpretation of the results and applying the concept to novel probe development is seriously hampered. The process from aggregation to disaggregation generally causes a recovery or enhancement of fluorescence signals, and thus provides an interesting new path to design "turn-on" probes. This tutorial review will provide the balanced comparison between aggregation and disaggregation mechanism, and focuses on the less explored advantages of "disaggregation" as a novel sensing mechanism and its recent applications in probe development.
Assuntos
Sondas Moleculares/química , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/metabolismo , DNA/química , DNA/metabolismo , Corantes Fluorescentes/química , Metais/química , Sondas Moleculares/metabolismo , Nanopartículas/químicaRESUMO
The first fluorescent sensor (GHB Orange) for date rape drug GHB was developed. It exhibits the fluorescence quenching property for GHB and allows its detection in various drinks. The interaction mechanism was elucidated as intramolecular charge transfer induced by a hydrogen bond. This discovery will help in solving the drug facilitated sexual assault problems.
Assuntos
Bebidas/análise , Depressores do Sistema Nervoso Central/análise , Corantes Fluorescentes/química , Hidroxibutiratos/análise , Drogas Ilícitas/análise , Depressores do Sistema Nervoso Central/química , Ligação de Hidrogênio , Hidroxibutiratos/química , Drogas Ilícitas/química , EstuproRESUMO
The dynamics of cellular heat production and propagation remains elusive at a subcellular level. Here we report the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum (ER thermo yellow), with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca(2+) changes in HeLa cells. Our thermometer anchored at nearly-zero distance from the ER, i.e. the heat source, allowed the detection of the heat as it readily dissipated, and revealed the dynamics of heat production in real time at a subcellular level.
Assuntos
Retículo Endoplasmático/metabolismo , Corantes Fluorescentes/metabolismo , Temperatura , Termômetros , Citoplasma/metabolismo , Corantes Fluorescentes/química , Células HeLa , Humanos , Nanopartículas/químicaRESUMO
Novel structures of an near-infrared (NIR) tetraarylazadipyrromethene (aza-BODIPY) series have been prepared. We designed the core structure containing two amido groups at the para-position of the aromatic rings. The amido group was incorporated to secure insensitivity to pH and to ensure a bathochromic shift to the NIR region. Forty members of aza-BODIPY compounds were synthesized by substitution of the acetyl group with commercial amines on the alpha bromide. The physicochemical properties and photostability were investigated and the fluorescence emission maxima (745~755 nm) were found to be in the near infrared (NIR) range of fluorescence.
RESUMO
Methods for the isolation of live neural stem cells from the brain are limited due to the lack of well-defined cell surface markers and tools to detect intracellular markers. To date most methods depend on the labeling of extracellular markers using antibodies, with intracellular markers remaining inaccessible in live cells. Using a novel intracellular protein FABP7 (Fatty Acid Binding Protein-7) selective fluorescent chemical probe CDr3, we have successfully isolated high FABP7 expressing cells from the embryonic and adult mouse brains. These cells are capable of forming neurospheres in culture, express neural stem cell marker genes and differentiate into neurons, astrocytes and oligodendrocytes. Characterization of cells sorted with Aldefluor or antibodies against CD133 or SSEA-1 showed that the cells isolated by CDr3 exhibit a phenotype distinct from the cells sorted with conventional methods. FABP7 labeling with CDr3 represents a novel method for rapid isolation of neural stem cells based on the expression of a single intracellular marker.
Assuntos
Compostos de Boro/metabolismo , Encéfalo/citologia , Separação Celular/métodos , Proteínas de Ligação a Ácido Graxo/metabolismo , Corantes Fluorescentes/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Células-Tronco Neurais/citologia , Antígeno AC133 , Animais , Antígenos CD/imunologia , Antígenos CD/metabolismo , Astrócitos/citologia , Astrócitos/metabolismo , Compostos de Boro/química , Diferenciação Celular , Proteína 7 de Ligação a Ácidos Graxos , Corantes Fluorescentes/química , Glicoproteínas/imunologia , Glicoproteínas/metabolismo , Antígenos CD15/imunologia , Antígenos CD15/metabolismo , Camundongos , Células-Tronco Neurais/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Oligodendroglia/citologia , Oligodendroglia/metabolismo , Peptídeos/imunologia , Peptídeos/metabolismo , Fenótipo , Ligação ProteicaRESUMO
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
Assuntos
Corantes Fluorescentes/química , Ensaios de Triagem em Larga Escala , Preparações Farmacêuticas/química , Albumina Sérica/química , Sítios de Ligação , Humanos , Modelos Moleculares , Estrutura MolecularRESUMO
A novel ratiometric biothiol probe Glutathione Green was developed. It allows quantitative measurement of glutathione in cell extracts and direct visualization of changes in glutathione levels in live cells. Remarkably, this is the first reported biothiol probe which can detect the carcinoma region of liver tissue based on the differences in the glutathione level.
Assuntos
Compostos de Boro/química , Corantes Fluorescentes/química , Glutationa/análise , Neoplasias Hepáticas/patologia , Animais , Células HeLa , Humanos , Neoplasias Hepáticas/metabolismo , Microscopia de Fluorescência , RatosRESUMO
The first fluorescent sensor for an illicit date rape drug, GBL, was developed and named Green Date. It shows high fluorescence enhancement to GBL and allows its detection in different drinks. The mechanism between GBL and Green Date was explored. This discovery may help to prevent the drug-facilitated sexual assault problems.
Assuntos
4-Butirolactona/análise , Bebidas/análise , Drogas Ilícitas/análise , Detecção do Abuso de Substâncias/métodos , Compostos de Boro/química , Fluorescência , Corantes Fluorescentes/química , Humanos , EstuproRESUMO
Caffeine has attracted abundant attention due to its extensive existence in beverages and medicines. However, to detect it sensitively and conveniently remains a challenge, especially in resource-limited regions. Here we report a novel aqueous phase fluorescent caffeine sensor named Caffeine Orange which exhibits 250-fold fluorescence enhancement upon caffeine activation and high selectivity. Nuclear magnetic resonance spectroscopy and Fourier transform infrared spectroscopy indicate that π-stacking and hydrogen-bonding contribute to their interactions while dynamic light scattering and transmission electron microscopy experiments demonstrate the change of Caffeine Orange ambient environment induces its fluorescence emission. To utilize this probe in real life, we developed a non-toxic caffeine detection kit and tested it for caffeine quantification in various beverages. Naked-eye sensing of various caffeine concentrations was possible based on color changes upon irradiation with a laser pointer. Lastly, we performed the whole system on a microfluidic device to make caffeine detection quick, sensitive and automated.
Assuntos
Cafeína/química , Corantes Fluorescentes/química , Técnicas Biossensoriais , Técnicas Analíticas Microfluídicas , Ressonância Magnética Nuclear Biomolecular , Sensibilidade e Especificidade , Espectroscopia de Infravermelho com Transformada de Fourier , Água/químicaRESUMO
We prepared a new library of 160 compounds by conjugation of a BODIPY core to a collection of aldehydes. This library was screened against 52 biologically relevant analytes and we identified one fluorescent sensor of fructose (Fructose Orange). Fructose Orange showed a 24-fold fluorescence increase upon recognition of fructose and an outstanding selectivity among 24 different saccharides. NMR studies confirmed that five different binding interactions were formed between the sensor and fructose. Furthermore, Fructose Orange was applied to the quantification of fructose in soft drinks, being the most selective fluorescent sensor for fructose reported to date.
Assuntos
Compostos de Boro/química , Corantes Fluorescentes/química , Frutose/análise , Espectrometria de Fluorescência/métodos , Bebidas/análise , Compostos de Boro/síntese química , Corantes Fluorescentes/síntese química , Sensibilidade e EspecificidadeRESUMO
A bodipy probe was developed for site-specific labeling of tagged proteins inside live cells which displays a large spectral change upon covalent coupling to the designed peptide that contains two pairs of Arg-Cys.
Assuntos
Acrilatos/química , Compostos de Boro/química , Imagem Molecular/métodos , Sondas Moleculares/química , Proteínas/química , Proteínas/metabolismo , Sequência de Aminoácidos , Sobrevivência Celular , Células HEK293 , Humanos , Proteínas Luminescentes/química , Proteínas Luminescentes/metabolismo , Modelos Moleculares , Estrutura Secundária de Proteína , Proteína Vermelha FluorescenteRESUMO
The diversification of the BODIPY scaffold has been hindered by its controversial adaptability to solid-phase chemistry. Herein we report the first solid-phase synthesis of a BODIPY library in high purities. We screened the library against a set of proteins, identified an immunoglobulin fluorescent sensor (Ig Orange) and confirmed its binding by SPR experiments.