Quorum quenching affects biofilm development in an anaerobic membrane bioreactor (AnMBR): from macro to micro perspective.

The first experimental study on the influence of acyl homoserine lactones (AHLs) degrading quorum quenching (QQ) consortium on the dynamics of biofilm bio-communities (i.e., from suspended biomass to initial biofilm and mature biofilm) in an anaerobic membrane bioreactor (AnMBR) at a microscopic scale (denoted as QQAnMBR) was reported. QQ did not change the overall bacterial community of the suspended biomass, inclusive of the key functional bacteria. Moreover, the retarded initial biofilm formation was attributed to not only the lower extracellular polymeric substance content of suspended biomass, but also the decelerated colonization of the AHL-regulated low-abundance in suspended biomass but pioneering keystone taxa Rhodocyclaceae;g- on membrane surface. However, pioneering fouling-related taxa such as Sulfurovum and Rhodocyclaceae;g- still played paramount roles in the delayed initial biofilm formation in the QQAnMBR. Furthermore, the microbial assemblies of the mature biofilm were changed in the QQAnMBR, probably attributable to the abiotic microbial floc attachment.

Effect of quorum quenching on EPS and size-fractioned particles and organics in anaerobic membrane bioreactor for domestic wastewater treatment.

Quorum quenching (QQ) has been applied as a promising membrane fouling control strategy for anaerobic membrane bioreactors (AnMBRs). Nevertheless, long-term operation of AnMBRs for real domestic wastewater (DWW) treatment needs to be systematically studied to evaluate comprehensive membrane fouling mechanisms and bioprocess performance. In this study, the impact of QQ on membrane fouling was investigated using a quorum quenching AnMBR (QQAnMBR) deploying a bead-entrapped facultative quorum quenching consortium (FQQ) to treat DWW. FQQ was shown to prolong membrane filtration operation by an average of 75%. Reduced proteins (p < 0.005) and carbohydrates (p < 0.005) in the extracellular polymeric substances (EPS) of mixed liquor (ML) were key differentiators that led to lower cake layer (CL) formation. Additionally, reduced biopolymers production (p < 0.05) in EPS improved sludge dewaterability. The findings suggested that QQ could alter fluorescent microbial metabolites of both EPS and CL as unveiled by excitation-emission matrix spectra pattern. Furthermore, colloidal particles (i.e., particles with size larger than 0.45 µm in ML supernatants) production was retarded by QQ, thereafter, also contributed to the reduced CL formation. Pore blockage was slightly increased by QQ, which might be attributed to pore blockage by large (∼230 nm) and small organic compounds (∼51 nm) in soluble microbial products (SMP). However, QQ had no significant impact on organic concentration of SMP, and QQ was not associated with particle size distribution of biomass. QQ performance was further affirmed through suppressed production of C4-HSL, 3-OXO-C6-HSL, and C6-HSL. The overall AHLs degradability of FQQ was well-maintained even after five membrane service cycles (total operation of 70 d). Moreover, QQ had no compromised impact on treatment performance (i.e., chemical oxygen demand (COD) removal and methane yield). Collectively, this study bridged the knowledge gap to bring forward QQ technology in AnMBR for widespread domestic wastewater treatment application.