Formation, application, and storage-reactivation of aerobic granular sludge: A review.

It was an important discovery in wastewater treatment that the microorganisms in the traditional activated sludge can form aerobic granular sludge (AGS) by self-aggregation under appropriate water quality and operation conditions. With a typical three-dimensional spherical structure, AGS has high sludge-water separation efficiency, great treatment capacity, and strong tolerance to toxic and harmful substances, so it has been considered to be one of the most promising wastewater treatment technologies. This paper comprehensively reviewed AGS from multiple perspectives over the past two decades, including the culture conditions, granulation mechanisms, metabolic and structural stability, storage, and its diverse applications. Some important issues, such as the reproducibility of culture conditions and the structural and functional stability during application and storage, were also summarized, and the research prospects were put forward. The aggregation behavior of microorganisms in AGS was explained from the perspectives of physiology and ecology of complex populations. The storage of AGS is considered to have large commercial potential value with the increase of large-scale applications. The purpose of this paper is to provide a reference for the systematic and in-depth study on the sludge aerobic granulation process.

Application of MicroResp™ for quick and easy detection of plastic degradation by marine bacterial isolates.

Microplastic debris in the marine environment is a global problem. Biodegradable polymers are being developed as alternatives to petroleum-based plastics, and quick and easy methods for screening for bacterial strains that can degrade such polymers are needed. As a screening method, the clear zone method has been widely used but has technical difficulties such as plate preparation and interpretation of results. In this study, we adapted the MicroResp™ system to easily detect biodegradation activity of marine bacteria in a 3-day assay. Among the 6 bacterial strains tested, 3, 2 and 1 strain degraded poly (butylene succinate-co-adipate) (PBSA), poly (ε-caprolactone) (PCL) and poly (3-hydroxybutyrate-co-3-hydroxyhexanoate), respectively. Only one strain that showed degradation activity of PBSA and PCL in the MicroResp™ system was also positive in the clear zone assay on the respective emulsion plates. Our results show that the adapted MicroResp™ system can screen for bacterial strains that degrade plastic.

Biodegradation of polysaccharide-based biocomposites with acetylated cellulose nanocrystals, alginate and chitosan in aqueous environment.

Biocomposite films from renewable sources are seen to be viable candidates as sustainable, zero-waste packaging materials. In this study, biocomposites films using chitosan and alginate as matrices, and pristine or acetylated cellulose nanocrystals (CNCs) as reinforcement agents, were fabricated, thoroughly characterized in terms of structure (with ATR-FTIR and XRD), morphology (SEM), thermal stability (TGA coupled with FTIR), water content and solubility and mechanical properties and subjected to controlled biological degradation in aqueous environment with added activated sludge. Biodegradation activity was followed through respirometry by measurement of change in partial O2 pressure using OxiTop® system. While the initial rate of biodegradation is higher in chitosan-based films with incorporated CNCs (both pristine and modified) compared to any other tested biocomposites, it was observed that chitosan-based films are not completely degradable in activated sludge medium, whereas alginate-based films reached complete biodegradation in 107 h to 112 h. Additional study of the aqueous medium with in situ FTIR during biodegradation offered an insight into biodegradation mechanisms. Use of advanced statistical methods indicated that selection of material (ALG vs CH) has the highest influence on biodegradability, followed by solubility of the material and its thermal stability.