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1.
Methods Mol Biol ; 2654: 345-362, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37106193

RESUMEN

The study of lymphocyte polarization upon antigen encounter typically relies on the random pairing between the cells of interest and a stimulating particle (micro bead) that mimics only some of the properties of the antigen-presenting cells. Here, we show how to build and use a microfluidic chip that allows to multiplex and synchronize the encounter between a lymphocyte and an antigen-presenting object: a functionalized oil-in-water droplet. We also explain how to fabricate and functionalize lipid droplets, an antigen-presenting tool that is, at the same time, deformable, fluid, and spherical.


Asunto(s)
Gotas Lipídicas , Microfluídica , Polaridad Celular , Agua , Microesferas
2.
Curr Top Dev Biol ; 154: 197-222, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37100518

RESUMEN

Immune cells are responsible for pathogen detection and elimination, as well as for signaling to other cells the presence of potential danger. In order to mount an efficient immune response, they need to move and search for a pathogen, interact with other cells, and diversify the population by asymmetric cell division. All these actions are regulated by cell polarity: cell polarity controls cell motility, which is crucial for scanning peripheral tissues to detect pathogens, and recruiting immune cells to sites of infection; immune cells, in particular lymphocytes, communicate with each other by a direct contact called immunological synapse, which entails a global polarization of the cell and plays a role in activating lymphocyte response; finally, immune cells divide asymmetrically from a precursor, generating a diversity of phenotypes and cell types among daughter cells, such as memory and effector cells. This review aims at providing an overview from both biology and physics perspectives of how cell polarity shapes the main immune cell functions.


Asunto(s)
División Celular Asimétrica , Transducción de Señal , Linfocitos , Polaridad Celular
3.
Cell Rep Methods ; 2(11): 100335, 2022 11 21.
Artículo en Inglés | MEDLINE | ID: mdl-36452873

RESUMEN

The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formation of the synapse, their impact on polarization has not yet been studied. Using functional lipid droplets as tunable artificial presenting cells combined with a microfluidic pairing device, we simultaneously observe synchronized synapses and dynamically quantify polarization patterns of individual B cells. By assessing how ligand concentration, surface fluidity, and substrate rigidity impact lysosome polarization, we show that its onset and kinetics depend on the local antigen concentration at the synapse and on substrate rigidity. Our experimental system enables a fine phenotyping of monoclonal cell populations based on their synaptic readout.


Asunto(s)
Gotas Lipídicas , Microfluídica , Gotas Lipídicas/metabolismo , Sinapsis Inmunológicas , Transducción de Señal , Linfocitos B , Antígenos/metabolismo
4.
Elife ; 112022 09 16.
Artículo en Inglés | MEDLINE | ID: mdl-36111670

RESUMEN

Immune synapse formation is a key step for lymphocyte activation. In B lymphocytes, the immune synapse controls the production of high-affinity antibodies, thereby defining the efficiency of humoral immune responses. While the key roles played by both the actin and microtubule cytoskeletons in the formation and function of the immune synapse have become increasingly clear, how the different events involved in synapse formation are coordinated in space and time by actin-microtubule interactions is not understood. Using a microfluidic pairing device, we studied with unprecedented resolution the dynamics of the various events leading to immune synapse formation and maintenance in murine B cells. Our results identify two groups of events, local and global, dominated by actin and microtubules dynamics, respectively. They further highlight an unexpected role for microtubules and the GEF-H1-RhoA axis in restricting F-actin polymerization at the lymphocyte-antigen contact site, thereby allowing the formation and maintenance of a unique competent immune synapse.


Asunto(s)
Actinas , Microtúbulos , Ratones , Animales , Factores de Intercambio de Guanina Nucleótido Rho , Polimerizacion , Linfocitos B , Sinapsis
5.
J Vis Exp ; (161)2020 07 23.
Artículo en Inglés | MEDLINE | ID: mdl-32773764

RESUMEN

Traction force microscopy (TFM) enables the measurement of forces produced by a cell on a substrate. This technique infers traction force measurements from an experimentally observed displacement field produced by a cell pulling on an elastic substrate. Here, we adapted TFM to investigate the spatial and temporal structure of the force field exerted by B cells when activated by antigen engagement of the B cell receptor. Gel rigidity, bead density, and protein functionalization must be optimized for the study of relatively small cells (~ 6 µm) that interact with, and respond specifically to ligands for cell surface receptors.


Asunto(s)
Activación de Linfocitos/fisiología , Microscopía de Fuerza Atómica/métodos , Humanos
6.
Sci Signal ; 13(627)2020 04 14.
Artículo en Inglés | MEDLINE | ID: mdl-32291315

RESUMEN

Diacylglycerol kinases (DGKs) limit antigen receptor signaling in immune cells by consuming the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA). Here, we showed that DGKζ promotes lymphocyte function-associated antigen 1 (LFA-1)-mediated adhesion and F-actin generation at the immune synapse of B cells with antigen-presenting cells (APCs), mostly in a PA-dependent manner. Measurement of single-cell mechanical force generation indicated that DGKζ-deficient B cells exerted lower forces at the immune synapse than did wild-type B cells. Nonmuscle myosin activation and translocation of the microtubule-organizing center (MTOC) to the immune synapse were also impaired in DGKζ-deficient B cells. These functional defects correlated with the decreased ability of B cells to present antigen and activate T cells in vitro. The in vivo germinal center response of DGKζ-deficient B cells was also reduced compared with that of wild-type B cells, indicating that loss of DGKζ in B cells impaired T cell help. Together, our data suggest that DGKζ shapes B cell responses by regulating actin remodeling, force generation, and antigen uptake-related events at the immune synapse. Hence, an appropriate balance in the amounts of DAG and PA is required for optimal B cell function.


Asunto(s)
Linfocitos B/metabolismo , Citoesqueleto/inmunología , Diacilglicerol Quinasa/inmunología , Sinapsis Inmunológicas/inmunología , Animales , Citoesqueleto/genética , Diacilglicerol Quinasa/genética , Sinapsis Inmunológicas/genética , Ratones , Ratones Noqueados
7.
J Cell Sci ; 133(5)2020 03 02.
Artículo en Inglés | MEDLINE | ID: mdl-32122988

RESUMEN

Studies in recent years have shed light on the particular features of cytoskeleton dynamics in immune cells, challenging the classical picture drawn from typical adherent cell lines. New mechanisms linking the dynamics of the membrane-cytoskeleton interface to the mechanical properties of immune cells have been uncovered and shown to be essential for immune surveillance functions. In this Essay, we discuss these features, and propose immune cells as a new playground for cell biologists who try to understand how cells adapt to different microenvironments to fulfil their functions efficiently.


Asunto(s)
Citoesqueleto
8.
Nat Commun ; 10(1): 2870, 2019 06 28.
Artículo en Inglés | MEDLINE | ID: mdl-31253773

RESUMEN

An important channel of cell-to-cell communication is direct contact. The immune synapse is a paradigmatic example of such type of interaction: it forms upon engagement of antigen receptors in lymphocytes by antigen-presenting cells and allows the local exchange of molecules and information. Although mechanics has been shown to play an important role in this process, how forces organize and impact on synapse function is unknown. We find that mechanical forces are spatio-temporally patterned at the immune synapse: global pulsatile myosin II-driven tangential forces are observed at the synapse periphery while localised forces generated by invadosome-like F-actin protrusions are detected at its centre. Noticeably, we observe that these force-producing actin protrusions constitute the main site of antigen extraction and endocytosis and require myosin II contractility to form. The interplay between global and local forces dictated by the organization of the actomyosin cytoskeleton therefore controls endocytosis at the immune synapse.


Asunto(s)
Citoesqueleto de Actina/fisiología , Actomiosina/metabolismo , Linfocitos B/fisiología , Endocitosis/fisiología , Miosina Tipo II/metabolismo , Actomiosina/genética , Animales , Comunicación Celular , Cruzamientos Genéticos , Integrasas/genética , Integrasas/metabolismo , Ratones , Ratones Noqueados , Ratones Transgénicos , Miosina Tipo II/genética , Receptores de Complemento 3d
9.
J Cell Biol ; 217(12): 4092-4105, 2018 12 03.
Artículo en Inglés | MEDLINE | ID: mdl-30348749

RESUMEN

Caveolae are small invaginated pits that function as dynamic mechanosensors to buffer tension variations at the plasma membrane. Here we show that under mechanical stress, the EHD2 ATPase is rapidly released from caveolae, SUMOylated, and translocated to the nucleus, where it regulates the transcription of several genes including those coding for caveolae constituents. We also found that EHD2 is required to maintain the caveolae reservoir at the plasma membrane during the variations of membrane tension induced by mechanical stress. Metal-replica electron microscopy of breast cancer cells lacking EHD2 revealed a complete absence of caveolae and a lack of gene regulation under mechanical stress. Expressing EHD2 was sufficient to restore both functions in these cells. Our findings therefore define EHD2 as a central player in mechanotransduction connecting the disassembly of the caveolae reservoir with the regulation of gene transcription under mechanical stress.


Asunto(s)
Proteínas Portadoras/metabolismo , Caveolas/metabolismo , Mecanotransducción Celular , Estrés Mecánico , Transcripción Genética , Proteínas Portadoras/genética , Células HeLa , Humanos
10.
J Cell Biol ; 216(1): 17-19, 2017 01 02.
Artículo en Inglés | MEDLINE | ID: mdl-27998992

RESUMEN

Using an exquisite cell imaging approach based on DNA nanosensors, Spillane and Tolar (2016. J. Cell Biol. https://doi.org/10.1083/jcb.201607064) explore how the physical properties of antigen-presenting cell surfaces affect how B cells internalize surface-tethered antigens. Soft and flexible surfaces promote mechanical force-mediated antigen extraction, whereas stiff surfaces lead to enzyme-mediated antigen release before subsequent internalization.


Asunto(s)
Antígenos de Superficie , Receptores de Antígenos de Linfocitos B/inmunología , Células Presentadoras de Antígenos , Antígenos/inmunología , Linfocitos B/inmunología , Humanos
11.
Immunol Rev ; 272(1): 39-51, 2016 07.
Artículo en Inglés | MEDLINE | ID: mdl-27319341

RESUMEN

Antigen presentation refers to the ability of cells to show MHC-associated determinants to T lymphocytes, leading to their activation. MHC class II molecules mainly present peptide-derived antigens that are internalized by endocytosis in antigen-presenting cells (APCs). Here, we describe how the interface between cellular membranes and the cytoskeleton regulates the various steps that lead to the presentation of exogenous antigens on MHC class II molecules in the two main types of APCs: dendritic cells (DCs) and B lymphocytes. This includes antigen uptake, processing, APC migration, and APC-T cell interactions. We further discuss how the interaction between APC-specific molecules and cytoskeleton elements allows the coordination of antigen presentation and cell migration in time and space.


Asunto(s)
Presentación de Antígeno , Linfocitos B/inmunología , Membrana Celular/metabolismo , Citoesqueleto/metabolismo , Células Dendríticas/inmunología , Linfocitos T/inmunología , Animales , Antígenos/metabolismo , Movimiento Celular , Endocitosis , Antígenos de Histocompatibilidad/metabolismo , Humanos , Sinapsis Inmunológicas , Activación de Linfocitos , Péptidos/metabolismo
13.
Nat Cell Biol ; 18(1): 43-53, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26641718

RESUMEN

Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA-mDia1-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42-Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4-MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDia1-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.


Asunto(s)
Actinas/metabolismo , Diferenciación Celular/fisiología , Movimiento Celular/fisiología , Quimiotaxis/fisiología , Células Dendríticas/metabolismo , Animales , Células Cultivadas , Ratones
14.
Mol Biol Cell ; 26(7): 1273-85, 2015 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-25631815

RESUMEN

B-cell receptor (BCR) engagement with surface-tethered antigens leads to the formation of an immune synapse, which facilitates antigen uptake for presentation to T-lymphocytes. Antigen internalization and processing rely on the early dynein-dependent transport of BCR-antigen microclusters to the synapse center, as well as on the later polarization of the microtubule-organizing center (MTOC). MTOC repositioning allows the release of proteases and the delivery of MHC class II molecules at the synapse. Whether and how these events are coordinated have not been addressed. Here we show that the ancestral polarity protein Par3 promotes BCR-antigen microcluster gathering, as well as MTOC polarization and lysosome exocytosis, at the synapse by facilitating local dynein recruitment. Par3 is also required for antigen presentation to T-lymphocytes. Par3 therefore emerges as a key molecule in the coupling of the early and late events needed for efficient extraction and processing of immobilized antigen by B-cells.


Asunto(s)
Presentación de Antígeno/inmunología , Antígenos/metabolismo , Linfocitos B/inmunología , Moléculas de Adhesión Celular/fisiología , Sinapsis Inmunológicas/inmunología , Receptores de Antígenos de Linfocitos B/metabolismo , Proteínas Adaptadoras Transductoras de Señales , Animales , Antígenos/inmunología , Linfocitos B/metabolismo , Proteínas de Ciclo Celular , Ratones , Receptores de Antígenos de Linfocitos B/inmunología
15.
Mol Biol Cell ; 25(20): 3195-209, 2014 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-25143404

RESUMEN

Membrane fusion underlies multiple processes, including exocytosis of hormones and neurotransmitters. Membrane fusion starts with the formation of a narrow fusion pore. Radial expansion of this pore completes the process and allows fast release of secretory compounds, but this step remains poorly understood. Here we show that inhibiting the expression of the small GTPase Cdc42 or preventing its activation with a dominant negative Cdc42 construct in human neuroendocrine cells impaired the release process by compromising fusion pore enlargement. Consequently the mode of vesicle exocytosis was shifted from full-collapse fusion to kiss-and-run. Remarkably, Cdc42-knockdown cells showed reduced membrane tension, and the artificial increase of membrane tension restored fusion pore enlargement. Moreover, inhibiting the motor protein myosin II by blebbistatin decreased membrane tension, as well as fusion pore dilation. We conclude that membrane tension is the driving force for fusion pore dilation and that Cdc42 is a key regulator of this force.


Asunto(s)
Membrana Celular/metabolismo , Exocitosis/fisiología , Fusión de Membrana/fisiología , Vesículas Secretoras/metabolismo , Proteína de Unión al GTP cdc42/metabolismo , Transporte Biológico , Humanos , Miosina Tipo II/metabolismo
16.
Nat Rev Immunol ; 13(7): 475-86, 2013 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-23797063

RESUMEN

B cells are key components of the adaptive immune response. Their differentiation into either specific memory B cells or antibody-secreting plasma cells is a consequence of activation steps that involve the processing and presentation of antigens. The engagement of B cell receptors by surface-tethered antigens leads to the formation of an immunological synapse that coordinates cell signalling events and that promotes antigen uptake for presentation on MHC class II molecules. In this Review, we discuss membrane trafficking and the associated molecular mechanisms that are involved in antigen extraction and processing at the B cell synapse, and we highlight how B cells use cell polarity to coordinate the complex events that ultimately lead to efficient humoral responses.


Asunto(s)
Presentación de Antígeno/inmunología , Linfocitos B/inmunología , Polaridad Celular/inmunología , Receptores de Antígenos de Linfocitos B/inmunología , Animales , Linfocitos B/citología , Antígenos de Histocompatibilidad Clase II/inmunología , Humanos , Sinapsis Inmunológicas/inmunología , Activación de Linfocitos/inmunología
17.
Nucleic Acids Res ; 41(9): 4926-37, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23543461

RESUMEN

During the past years, exogenous DNA molecules have been used in gene and molecular therapy. At present, it is not known how these DNA molecules reach the cell nucleus. We used an in cell single-molecule approach to observe the motion of exogenous short DNA molecules in the cytoplasm of eukaryotic cells. Our observations suggest an active transport of the DNA along the cytoskeleton filaments. We used an in vitro motility assay, in which the motion of single-DNA molecules along cytoskeleton filaments in cell extracts is monitored; we demonstrate that microtubule-associated motors are involved in this transport. Precipitation of DNA-bound proteins and mass spectrometry analyses reveal the preferential binding of the kinesin KIFC1 on DNA. Cell extract depletion of kinesin KIFC1 significantly decreases DNA motion, confirming the active implication of this molecular motor in the intracellular DNA transport.


Asunto(s)
ADN/metabolismo , Cinesinas/metabolismo , Transporte Biológico Activo , Citoesqueleto/metabolismo , ADN/análisis , Dineínas/metabolismo , Células HeLa , Humanos , Cinesinas/análisis , Microscopía Fluorescente , Microscopía de Contraste de Fase , Microtúbulos/metabolismo
18.
Adv Drug Deliv Rev ; 64(2): 167-78, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-21729726

RESUMEN

In the last two decades, the single particle and single molecule approach became more and more popular to investigate the activity and the mechano-chemical properties of biological molecules. The inherent limit of these assays was that the molecules of interest were observed in vitro, out of their natural environment, the cell. Several recent works have shown the possibility to overcome this limit, to extend this approach to living cells and to observe the details of many cellular processes at the molecular level. In this review we discuss the use of semiconductor quantum dots to perform single particle and single molecule tracking in the cell. We refer to other articles for the technical aspects of this method. Here, after an introduction on the advantages provided by these nanoparticles, we restrict ourselves to some examples, mainly related to intracellular transport and molecular motor activity. These will illustrate the important role played by semiconductor quantum dots as fluorescent nano-reporters in in cell single molecule approach in modern biology and biophysics.


Asunto(s)
Técnicas Citológicas , Puntos Cuánticos , Animales , Fenómenos Fisiológicos Celulares , Semiconductores
19.
Immunity ; 35(3): 361-74, 2011 Sep 23.
Artículo en Inglés | MEDLINE | ID: mdl-21820334

RESUMEN

Engagement of the B cell receptor (BCR) by surface-tethered antigens (Ag) leads to formation of a synapse that promotes Ag uptake for presentation onto major histocompatibility complex class II (MHCII) molecules. We have highlighted the membrane trafficking events and associated molecular mechanisms involved in Ag extraction and processing at the B cell synapse. MHCII-containing lysosomes are recruited to the synapse where they locally undergo exocytosis, allowing synapse acidification and the extracellular release of hydrolases that promote the extraction of the immobilized Ag. Lysosome recruitment and secretion results from the polarization of the microtubule-organizing center (MTOC), which relies on the cell division cycle (Cdc42)-downstream effector, atypical protein kinase C (aPKCζ). aPKCζ is phosphorylated upon BCR engagement, associates to lysosomal vesicles, and is required for their polarized secretion at the B cell synapse. Regulation of B lymphocyte polarity therefore emerges as a central mechanism that couples Ag extraction to Ag processing and presentation.


Asunto(s)
Presentación de Antígeno , Linfocitos B/inmunología , Sinapsis Inmunológicas , Lisosomas , Receptores de Antígenos de Linfocitos B/fisiología , Animales , Polaridad Celular , Lisosomas/metabolismo , Ratones , Proteína Quinasa C/inmunología , Receptores de Antígenos de Linfocitos B/inmunología , Proteína de Unión al GTP cdc42/inmunología
20.
Phys Rev E Stat Nonlin Soft Matter Phys ; 82(6 Pt 1): 061904, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-21230687

RESUMEN

Proteins involved in homologous recombination such as RecA and hRad51 polymerize on single- and double-stranded DNA according to a nucleation-growth kinetics, which can be monitored by single-molecule in vitro assays. The basic models currently used to extract biochemical rates rely on ensemble averages and are typically based on an underlying process of bidirectional polymerization, in contrast with the often observed anisotropic polymerization of similar proteins. For these reasons, if one considers single-molecule experiments, the available models are useful to understand observations only in some regimes. In particular, recent experiments have highlighted a steplike polymerization kinetics. The classical model of one-dimensional nucleation growth, the Kolmogorov-Avrami-Mehl-Johnson (KAMJ) model, predicts the correct polymerization kinetics only in some regimes and fails to predict the steplike behavior. This work illustrates by simulations and analytical arguments the limitation of applicability of the KAMJ description and proposes a minimal model for the statistics of the steps based on the so-called stick-breaking stochastic process. We argue that this insight might be useful to extract information on the time and length scales involved in the polymerization kinetics.


Asunto(s)
Modelos Biológicos , Multimerización de Proteína , Proteínas/química , Cinética , Estructura Cuaternaria de Proteína , Proteínas/metabolismo , Procesos Estocásticos , Termodinámica
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