RESUMEN
Time-temperature data for queso fresco (QF) cheese varieties stored in a residential refrigerator operating at 5°C and a predictive microbiology secondary model for Listeria monocytogenes in QF were used to estimate a refrigerator performance indicator (RPI) of microbial preservation. RPI values were used to assess how compressor technology (single [SS] and variable speed [VS]), ambient temperature (21.1°C [LT] and 32.2°C [HT]), and refrigerator load (22.5 kg regular load and 39 kg higher load) affected preservation performance. All deterministic and probabilistic RPI estimations slightly exceeded the desirable 1.0 value, i.e., the variable temperatures for the QF kept in the refrigerator were worse than keeping it constantly at the temperature recommended by food safety agencies for QF. Furthermore, the mean comparison of estimates of the time-temperature equivalent indicator previously developed by French researchers showed similar behavior to those observed for RPI. Finally, statistical analysis showed that Tambient was the factor with the highest impact on refrigerator performance because of its impact on the sample temperature increase during door openings and when exposed to ambient temperature during product use. This highlights the need to reduce the time for product temperature recovery by improving the compressor operation logic. Also important are consumer behavior changes such as a reduction in product exposure to ambient temperature and in the door opening duration and frequency. PRACTICAL APPLICATION: This study demonstrated how a quantitative tool (RPI) can assess refrigerator preservation performance. Although the findings presented can be applied to any cold chain segment, the data used was collected for its weakest link, the domestic refrigerator. Surveys show that 77% of them operate above the recommended 4°C. The RPI methodology is ready for use by refrigerator designers to assess performance improvements possible by modifications of the compressor operation logic. Moreover, it can be integrated into smart-hubs monitoring the frequency and duration of refrigerator door openings to inform consumers when their habits are compromising the preservation performance of the refrigerator.
RESUMEN
Home preservation depends on the food matrix, refrigerator design/technology, consumer actions, and ambient temperature. Storing different food matrices in product-relevant refrigerator locations generating different temperature histories can be used to develop an indicator of how refrigerator technology, consumer habits, and environment conditions impact the refrigerator food preservation performance. In this study, poultry, particularly prone to spoilage reflecting its pH, nutrient availability, and high aw, was used to evaluate refrigerator preservation performance as affected by compressor technology (single [SS] and variable speed [VS]), ambient temperature (21.1°C [LT] and 32.2°C [HT]), and refrigerator load (22.5 kg [RL] and 39 kg [HL]). Time-temperature values collected for chicken breast stored in a drawer independently controlled at 0°C in a refrigerator set 5°C, and a Pseudomonas predictive microbiology model, were used to estimate a normalized refrigerator performance indicator (RPI). Values <1, â¼1, and >1 described excellent, good, or poor performance, respectively. A first analysis revealed that up to 54% of chicken breast temperatures were above its recommended refrigerated storage value. When ignoring variability sources, SS technology yielded RPI values ranging 0.61-0.70, whereas the more energy efficient VS compressor yielded values ranging 0.86-1.14. The higher and wider VS RPI range reflects a compressor control logic optimized for energy efficiency compliance while disregarding effects on food preservation. When considering the variability of model parameters and temperature measurements through one-sided 95% confidence intervals yielded RPI reaching 1.16. Although the independently controlled drawer preservation performance was near optimal, it can improve by considering energy use and preservation impact when optimizing the compressor speed control protocol. PRACTICAL APPLICATION: Worldwide poultry meat consumption has reached 15 kg per person. Refrigeration is widely used for its safety and quality preservation. Efficiency regulations decreased the energy use of residential refrigerators by nearly tenfold even though their size increased by 50% in the last half century. In this study, we provide quantitative evidence that their preservation performance must be improved. This is particularly true for upper end units typically equipped with quieter and more energy-efficient variable speed compressors. The same methodology can be used to evaluate the preservation performance of the storage units, trucks, and display cases used for refrigerated products.
Asunto(s)
Pollos , Refrigeración , Animales , Temperatura , Refrigeración/métodos , Conservación de Alimentos/métodos , Microbiología de AlimentosRESUMEN
An undesirable crispiness loss occurs when some dry fruits reach a critical moisture content (Xc ) and their glass transition temperature (Tg ) matches the storage temperature. Models for sorption isotherms and onset Tg values for dry mango, apple, and banana were used to estimate Xc values at 25 and 32 °C. All models yielded R2 > 0.97 but information theory criteria strongly supported GAB in all but one case (40 °C, mango). The Gordon-Taylor Tg model (GT) yielded high R2 values for apple and banana but resulted in R2 = 0.834 for mango. As moisture approached zero, mango Tg estimates displayed a downward concavity contrasting with a rapidly increasing trend for apple and banana. The Khalloufi-Maslouhi-Ratti (KMR) model for Tg as a function of aw showed a linear behavior. Although the KMR model fitted data with R2 > 0.996, it requires more parameters and when aw approached 0, estimated Tg values increased at a slower rate than for the GT model. In the case of banana and mango, both models predicted approximately the same Xc at 25 °C but not at 32 °C. Finally, all Xc values estimated based on Tg were lower than the monolayer values obtained with the GAB (apple and banana) and BET (mango) models. These results indicate that the glass transition induced by moisture uptake dominates the quality degradation of these dry fruits.
RESUMEN
Sigmoidal microbial survival curves are observed in high-pressure carbon dioxide (HPCD) pasteurization treatments. The objectives of this study were to use the Gompertz primary model to describe the inactivation in apple juice of the pathogen Escherichia coli CGMCC1.90 and to apply probabilistic engineering to select HPCD treatments meeting at least 5 log10 reductions (SV ≥ 5) at 95% confidence. This required secondary models for the temperature (T, °C) and pressure (P, MPa) dependence of the Gompertz model parameters. The expressions [Formula: see text] and [Formula: see text] selected using goodness-of-fit measures and assessments based on Akaike and Bayesian information criteria were consistent with proposed mechanistic models for HPCD bactericidal effects. Monte Carlo simulations accounting for the variability and uncertainty of the parameter b and c estimates were used to predict SV values for a given time, temperature and CO2 pressure combination and desired confidence boundary. A similar approach used to estimate process times meeting SV ≥ 5 at 95% confidence for a given temperature and CO2 pressure combination, showed that HPCD processes met this requirement only for relatively long processing times, i.e., 35-124 min in the experimental range of 32-42 °C and 10-30 MPa. Therefore, further HPCD research is required to reduce processing time.
RESUMEN
Arthrospira platensis was used to obtain functional extracts through supercritical carbon dioxide extraction (SFE-CO2). Pressure (P), temperature (T), co-solvent (CX), static extraction (SX), dispersant (Di) and dynamic extraction (DX) were evaluated as process parameters through a Plackett-Burman design. The maximum extract yield obtained was 7.48 ± 0.15% w/w. The maximum contents of bioactive metabolites in extracts were 0.69 ± 0.09 µg/g of riboflavin, 5.49 ± 0.10 µg/g of α-tocopherol, 524.46 ± 0.10 µg/g of ß-carotene, 1.44 ± 0.10 µg/g of lutein and 32.11 ± 0.12 mg/g of fatty acids with 39.38% of palmitic acid, 20.63% of linoleic acid and 30.27% of γ-linolenic acid. A. platensis extracts had an antioxidant activity of 76.47 ± 0.71 µg GAE/g by Folin-Ciocalteu assay, 0.52 ± 0.02, 0.40 ± 0.01 and 1.47 ± 0.02 µmol TE/g by DPPH, FRAP and TEAC assays, respectively. These extracts showed antimicrobial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Overall, co-solvent was the most significant factor for all measured effects (p < 0.05). Arthrospira platensis represents a sustainable source of bioactive compounds through SFE using the following extraction parameters P: 450 bar, CX: 11 g/min, SX: 15 min, DX: 25 min, T: 60 °C and Di: 35 g.
Asunto(s)
Factores Biológicos/química , Dióxido de Carbono/química , Spirulina/química , Antiinfecciosos/química , Antiinfecciosos/farmacología , Antioxidantes/química , Antioxidantes/farmacología , Bacterias/efectos de los fármacos , Factores Biológicos/farmacología , Candida albicans/efectos de los fármacos , Ácidos Grasos/química , Ácidos Grasos/farmacología , Ácido Linoleico/química , Ácido Linoleico/farmacología , Luteína/química , Luteína/farmacología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Presión , Riboflavina/química , Riboflavina/farmacología , Solventes/química , Temperatura , alfa-Tocoferol/química , alfa-Tocoferol/farmacología , beta Caroteno/química , beta Caroteno/farmacologíaRESUMEN
Three alternatives for bioethanol production from pretreated mango stem bark after maceration (MSBAM) were evaluated as a biorefinery component for the mango agroindustry. These included separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and pre-saccharification followed by simultaneous saccharification and fermentation (PSSF). The effects on ethanol concentration, yield and productivity of pretreated MSBAM solids loading, Tween 20 addition, and temperature were used for process comparisons. The highest yields for the SHF, SSF, and PSSF process alternatives were 58.8, 81.6, and 84.5%, respectively. Since saccharification and fermentation are carried out in the same vessel in the SSF alternative, and no significant SSF and PSSF differences in ethanol concentration were observed, SSF is recommended as the best process configuration.
Asunto(s)
Biocombustibles , Mangifera , Etanol , Fermentación , Hidrólisis , TemperaturaRESUMEN
Clostridium difficile is frequently found in meat and meat products. Germination efficiency, defined as colony formation, was previously investigated at temperatures found in meat handling and processing for spores of strain M120 (animal isolate), R20291 (human isolate), and DK1 (beef isolate). In this study, germination efficiency of these spore strains was assessed in phosphate buffered saline (PBS, aw â¼1.00), commercial beef jerky (aw â¼0.82/0.72), and aw-adjusted PBS (aw â¼0.82/0.72). Surface hydrophobicity was followed for spores stored in PBS. After three months and for all PBS aw levels tested, M120 and DK1 spores showed a â¼1 decimal reduction in colony formation but this was not the case when kept in beef jerky suggesting a protective food matrix effect. During storage, and with no significant aw effect, an increase in colony formation was observed for R20291 spores kept in PBS (â¼2 decimal log increase) and beef jerky (â¼1 decimal log increase) suggesting a loss of spore superdormancy. For all strains, no significant changes in spore surface hydrophobicity were observed after storage. Collectively, these results indicate that depending on the germination properties of C. difficile spores and the media properties, their germination efficiency may increase or decrease during long term food storage.
Asunto(s)
Clostridioides difficile/fisiología , Esporas Bacterianas/efectos de los fármacos , Esporas Bacterianas/fisiología , Agua , Carga Bacteriana , Clostridioides difficile/efectos de los fármacos , Medios de Cultivo/química , Microbiología de Alimentos , Interacciones Hidrofóbicas e Hidrofílicas , Esporas Bacterianas/crecimiento & desarrollo , TemperaturaRESUMEN
A beta Poisson dose-response model for Vibrio vulnificus food poisoning cases leading to septicemia was used to evaluate the effect of depuration at 15 °C on the estimated health risk associated with raw oyster consumption. Statistical variability sources included V. vulnificus level at harvest, time and temperature during harvest and transportation to processing plants, decimal reductions (SV) observed during experimental circulation depuration treatments, refrigerated storage time before consumption, oyster size, and number of oysters per consumption event. Although reaching nondetectable V. vulnificus levels (<30 most probable number per gram) throughout the year and a 3.52 SV were estimated not possible at the 95% confidence level, depuration for 1, 2, 3, and 4 days would reduce the warm season (June through September) risk from 2,669 cases to 558, 93, 38, and 47 cases per 100 million consumption events, respectively. At the 95% confidence level, 47 and 16 h of depuration would reduce the warm and transition season (April through May and October through November) risk, respectively, to 100 cases per 100 million consumption events, which is assumed to be an acceptable risk; 1 case per 100 million events would be the risk when consuming untreated raw oysters in the cold season (December through March).
Asunto(s)
Ostreidae , Alimentos Crudos/microbiología , Vibriosis , Vibrio vulnificus , Animales , Conservación de Alimentos/métodos , Humanos , Factores de Riesgo , Estaciones del Año , Mariscos/microbiología , TemperaturaRESUMEN
High hydrostatic pressure (HHP) is the most-widely adopted novel non-thermal technology for the commercial pasteurization of foods. However, HHP-induced inactivation of bacterial spores remains a challenge due to spore resistance to the treatment limits of currently available industrial HHP units (i.e. ~650 MPa and 50 °C). Several reports have demonstrated that high pressure can modulate the germination machinery of bacterial spores, rendering them susceptible to subsequent inactivation treatments. Unfortunately, high pressure-induced germination is a unique phenomenon for spores of the genus Bacillus but not of Clostridium. Alternative strategies to inactivate bacterial spores at commercially available pressure and temperature levels include promoting the germination step by inclusion of known germinants into the food formulation to increase the lethality of HHP treatments on bacterial spores. The aim of this review is to provide an overview of the molecular basis involved in pressure-triggered germination of bacterial spores and of novel strategies to inactivate bacterial spores with HHP treatments.
Asunto(s)
Presión Hidrostática , Pasteurización/métodos , Esporas Bacterianas , Bacillus , Clostridium , Microbiología de AlimentosRESUMEN
Clostridium difficile's presence has been reported in meat products stored typically at low temperatures. This study evaluated the viability in phosphate buffer saline (PBS) of spores from epidemic C. difficile strain R20291 (4.6 log CFU/ml) and M120 (7.8 log CFU/ml). Viability was assessed during 4 months at -80 °C, -20 °C, 4 °C (refrigeration), and 23 °C (room temperature), and after 10 freeze (-20 °C)/thaw (+23 °C) cycles. Although spore viability decreased, significant viability was still observed after 4 months at -20 °C, i.e., 3.5 and 3.9 log CFU/ml and -80 °C, i.e., 6.0 and 6.1 log CFU/ml for strains R20291 and M120, respectively. The same trend was observed for M120 at 4 °C and 23 °C, while for R20291 the viability change was non-significant at 4 °C but increased significantly at 23 °C (p > 0.05). After 10 freeze-thaw cycles, viability of both strains decreased but a significant fraction remained viable (4.3 and 6.3 log CFU/ml for strain R20291 and M120, respectively). Strikingly, both strains showed higher viability in a meat model than in PBS. A small but significant decrease (p < 0.05) from 6.7 to 6.3 log CFU/ml in M120 viability was observed after 2-month storage in the meat model while the decrease from an initial 3.4 log CFU/ml observed for R20291 was non-significant (p = 0.12). In summary, C. difficile spores can survive low-temperature conditions for up to 4 months.
Asunto(s)
Clostridioides difficile/crecimiento & desarrollo , Viabilidad Microbiana , Frío , Productos de la Carne/microbiología , Esporas Bacterianas/crecimiento & desarrolloRESUMEN
UNLABELLED: Consumer demand for food safety and quality improvements, combined with new regulations, requires determining the processor's confidence level that processes lowering safety risks while retaining quality will meet consumer expectations and regulatory requirements. Monte Carlo calculation procedures incorporate input data variability to obtain the statistical distribution of the output of prediction models. This advantage was used to analyze the survival risk of Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) and Clostridium botulinum spores in high-temperature short-time (HTST) milk and canned mushrooms, respectively. The results showed an estimated 68.4% probability that the 15 sec HTST process would not achieve at least 5 decimal reductions in M. paratuberculosis counts. Although estimates of the raw milk load of this pathogen are not available to estimate the probability of finding it in pasteurized milk, the wide range of the estimated decimal reductions, reflecting the variability of the experimental data available, should be a concern to dairy processors. Knowledge of the C. botulinum initial load and decimal thermal time variability was used to estimate an 8.5 min thermal process time at 110 °C for canned mushrooms reducing the risk to 10â»9 spores/container with a 95% confidence. This value was substantially higher than the one estimated using average values (6.0 min) with an unacceptable 68.6% probability of missing the desired processing objective. Finally, the benefit of reducing the variability in initial load and decimal thermal time was confirmed, achieving a 26.3% reduction in processing time when standard deviation values were lowered by 90%. PRACTICAL APPLICATION: In spite of novel technologies, commercialized or under development, thermal processing continues to be the most reliable and cost-effective alternative to deliver safe foods. However, the severity of the process should be assessed to avoid under- and over-processing and determine opportunities for improvement. This should include a systematic approach to consider variability in the parameters for the models used by food process engineers when designing a thermal process. The Monte Carlo procedure here presented is a tool to facilitate this task for the determination of process time at a constant lethal temperature.
Asunto(s)
Agaricales , Clostridium botulinum tipo B/crecimiento & desarrollo , Alimentos en Conserva/microbiología , Leche/microbiología , Modelos Biológicos , Mycobacterium avium subsp. paratuberculosis/crecimiento & desarrollo , Pasteurización/métodos , Animales , Carga Bacteriana , Botulismo/prevención & control , Clostridium botulinum tipo B/aislamiento & purificación , Clostridium botulinum tipo B/fisiología , Almacenamiento de Alimentos , Cuerpos Fructíferos de los Hongos , Calor/efectos adversos , Cinética , Viabilidad Microbiana , Método de Montecarlo , Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación , Mycobacterium avium subsp. paratuberculosis/fisiología , Paratuberculosis/prevención & control , Control de Calidad , Reproducibilidad de los Resultados , Esporas Bacterianas/crecimiento & desarrollo , Esporas Bacterianas/aislamiento & purificación , Esporas Bacterianas/fisiologíaRESUMEN
The current study aimed to develop an inactivation strategy for Clostridium perfringens spores in meat through a combination of spore activation at low pressure (100-200 MPa, 7 min) and elevated temperature (80 degrees C, 10 min); spore germination at high temperatures (55, 60 or 65 degrees C); and inactivation of germinated spores with elevated temperatures (80 and 90 degrees C, 10 and 20 min) and high pressure (586 MPa, at 23 and 73 degrees C, 10 min). Low pressures (100-200 MPa) were insufficient to efficiently activate C. perfringens spores for germination. However, C. perfringens spores were efficiently activated with elevated temperature (80 degrees C, 10 min), and germinated at temperatures lethal for vegetative cells (>or= 55 degrees C) when incubated for 60 min with a mixture of L-asparagine and KCl (AK) in phosphate buffer (pH 7) and in poultry meat. Inactivation of spores (approximately 4 decimal reduction) in meat by elevated temperatures (80-90 degrees C for 20 min) required a long germination period (55 degrees C for 60 min). However, similar inactivation level was reached with shorter germination period (55 degrees C for 15 min) when spore contaminated-meat was treated with pressure-assisted thermal processing (568 MPa, 73 degrees C, 10 min). Therefore, the most efficient strategy to inactivate C. perfringens spores in poultry meat containing 50 mM AK consisted: (i) a primary heat treatment (80 degrees C, 10 min) to pasteurize and denature the meat proteins and to activate C. perfringens spores for germination; (ii) cooling of the product to 55 degrees C in about 20 min and further incubation at 55 degrees C for about 15 min for spore germination; and (iii) inactivation of germinated spores by pressure-assisted thermal processing (586 MPa at 73 degrees C for 10 min). Collectively, this study demonstrates the feasibility of an alternative and novel strategy to inactivate C. perfringens spores in meat products formulated with germinants specific for C. perfringens.
Asunto(s)
Clostridium perfringens/fisiología , Contaminación de Alimentos/análisis , Manipulación de Alimentos/métodos , Productos Avícolas/microbiología , Esporas Bacterianas/crecimiento & desarrollo , Animales , Asparagina/farmacología , Pollos , Recuento de Colonia Microbiana , Seguridad de Productos para el Consumidor , Contaminación de Alimentos/prevención & control , Microbiología de Alimentos , Conservación de Alimentos/métodos , Calor , Humanos , Presión Hidrostática , Cloruro de Potasio/farmacología , Factores de TiempoRESUMEN
Previous work showed that C. perfringens spores lacking the majority of alpha/beta-type small, acid-soluble spore proteins (SASPs) (termed alpha(-) beta(-) spores) exhibit greatly decreased resistance to moist heat and UV radiation. The current study demonstrated that these alpha(-) beta(-) spores had reduced resistance to hydrogen peroxide, hydrochloric acid, nitrous acid and formaldehyde. These results clearly demonstrate the important role of alpha/beta-type SASPs in the resistance of C. perfringens spores to chemicals.
Asunto(s)
Proteínas Bacterianas/química , Clostridium perfringens/efectos de la radiación , Calor , Esporas Bacterianas/química , Esporas Bacterianas/efectos de la radiación , Rayos Ultravioleta , Farmacorresistencia Bacteriana , Contaminación de Alimentos/prevención & controlRESUMEN
Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the important infectious cell morphotype, and after inoculation into a suitable host, these spores must germinate and return to active growth to cause GI disease. We have found differences in the germination of spores of C-cpe and P-cpe isolates in that (i) while a mixture of L-asparagine and KCl was a good germinant for spores of C-cpe and P-cpe isolates, KCl and, to a lesser extent, L-asparagine triggered spore germination in C-cpe isolates only; and (ii) L-alanine or L-valine induced significant germination of spores of P-cpe but not C-cpe isolates. Spores of a gerK mutant of a C-cpe isolate in which two of the proteins of a spore nutrient germinant receptor were absent germinated slower than wild-type spores with KCl, did not germinate with L-asparagine, and germinated poorly compared to wild-type spores with the nonnutrient germinants dodecylamine and a 1:1 chelate of Ca2+ and dipicolinic acid. In contrast, spores of a gerAA mutant of a C-cpe isolate that lacked another component of a nutrient germinant receptor germinated at the same rate as that of wild-type spores with high concentrations of KCl, although they germinated slightly slower with a lower KCl concentration, suggesting an auxiliary role for GerAA in C. perfringens spore germination. In sum, this study identified nutrient germinants for spores of both C-cpe and P-cpe isolates of C. perfringens and provided evidence that proteins encoded by the gerK operon are required for both nutrient-induced and non-nutrient-induced spore germination.
Asunto(s)
Proteínas Bacterianas/metabolismo , Clostridium perfringens/fisiología , Esporas Bacterianas/fisiología , Alanina/metabolismo , Alanina/farmacología , Asparagina/metabolismo , Asparagina/farmacología , Proteínas Bacterianas/genética , Cromosomas Bacterianos/genética , Clostridium perfringens/efectos de los fármacos , Clostridium perfringens/genética , Relación Dosis-Respuesta a Droga , Regulación Bacteriana de la Expresión Génica/efectos de los fármacos , Genes Bacterianos , Modelos Genéticos , Mutación , Operón/genética , Plásmidos/genética , Cloruro de Potasio/metabolismo , Cloruro de Potasio/farmacología , Protones , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Esporas Bacterianas/efectos de los fármacos , Esporas Bacterianas/genética , Temperatura , Valina/metabolismo , Valina/farmacologíaRESUMEN
Soluble proteins from surimi wash water (SWW) precipitated using a chitosan-alginate (Chi-Alg) complex and recovered by centrifugation were freeze-dried. Analysis showed that SWW proteins (SWWP) had a crude protein content of 73.1% and a high concentration of essential amino acids, for example, 3% histidine, 9.4% lysine, 3.7% methionine, and 5.1% phenylalanine. In a rat-feeding trial, SWWP as a single protein source showed higher (P < 0.05) modified protein efficiency ratio and net protein ratio than the casein control. Blood chemistry analysis revealed no deleterious effect from the full protein substitution or the chitosan in SWWP. Therefore, this preliminary study revealed that proteins recovered from SWW using the Chi-Alg complex could be used in feed formulations. They could be used for food production in countries where regulatory agencies allow the use of chitosan in the production of food ingredients.
Asunto(s)
Alginatos/química , Quitosano/química , Seguridad de Productos para el Consumidor , Proteínas/análisis , Aminoácidos Esenciales/análisis , Animales , Análisis Químico de la Sangre , Peces , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Masculino , Valor Nutritivo , Proteínas/normas , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Eliminación de Residuos Líquidos/métodos , Agua/químicaRESUMEN
Previous research has shown that soluble protein recovery by chitosan (Chi) complexes with polyanions such as alginate (Alg) is more effective than using chitosan alone. In this study, Chi-Alg complexes were used to recover soluble proteins from surimi wash water (SWW) slightly acidified to pH 6. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were used at 20, 40 and 100mg/L SWW Chi-Alg complexes prepared with a Chi:Alg mixing ratio previously optimized (MR=0.2). FTIR analysis of the solids recovered revealed the three characteristic amide bands observed in the same region for untreated SWW confirming protein adsorption by Chi-Alg. The superior effectiveness of Chi complexes was confirmed but differences among chitosan types could not be correlated to MW and DD. Experimental Chi samples with 94%, 93%, 75% and 93% DD and 22, 47, 225 and 3404 x 10(3)Da, respectively, showed 73-76% protein adsorption while a commercial chitosan sample with 84% DD and 3832 x 10(3)Da had 74-83% protein adsorption. An experimental chitosan, SY-1000 with 94% DD and 1.5 x 10(6)Da, showed the highest protein adsorption (79-86%) and turbidity reduction (85-92%) when used at 20mg/L SWW.
Asunto(s)
Alginatos/química , Quitosano/química , Peces , Proteínas/química , Agua/química , Animales , Ácido Glucurónico/química , Ácidos Hexurónicos/química , Eliminación de Residuos Líquidos/métodosRESUMEN
Surimi processors are seeking means to improve the utilization of seafood resources to increase productivity and also in response to the strong public pressure on this industry to reduce the organic matter in processing water discharged into the environment. Insoluble solids (IS) can be recovered from surimi wash water (SWW) by centrifugation. The quality implications of adding 0 (control), 1%, 3% and 5% of solids (SWW-IS) into surimi paste and gels were evaluated by determining their mechanical properties, moisture retention and color. This study showed that adding 1% SWW-IS improved the mechanical properties of Pacific whiting surimi with a minimal effect on color. Higher additions resulted in quality deterioration in mechanical properties and color.
Asunto(s)
Productos Pesqueros , Tecnología de Alimentos , Agua Dulce/química , Gadiformes , Animales , Color , Proteínas de Peces/química , Proteínas de Peces/aislamiento & purificación , SolubilidadRESUMEN
The effects of high hydrostatic pressure on volatile generation in milk were investigated in this study. Raw milk samples were treated under different pressures (482, 586, and 620 MPa), temperatures (25 and 60 degrees C), and holding times (1, 3, and 5 min). Samples submitted to heat treatments alone (25, 60, and 80 degrees C for 1, 3, and 5 min) were used for comparison. Trace volatile sulfur compounds were analyzed using solid-phase microextraction (SPME) and gas chromatography (GC) with pulsed-flame photometric detection (PFPD), whereas the rest of the volatile compounds were analyzed using SPME-GC with flame ionization detection (FID). Multivariate analysis of variance (MANOVA) and principal component analysis (PCA) were used to study the effect of pressure, temperature, and time on volatile generation. Relative concentration increases of 27 selected volatile compounds were compared to an untreated sample. It was found that pressure, temperature, and time, as well as their interactions, all had significant effects (P < 0.001) on volatile generation in milk. Pressure and time effects were significant at 60 degrees C, whereas their effects were almost negligible at 25 degrees C. The PCA plot indicated that the volatile generation of pressure-heated samples at 60 degrees C was different from that of heated-alone samples. Heat treatment tended to promote the formation of methanethiol, hydrogen sulfide, methyl ketones, and aldehydes, whereas high-pressure treatment favored the formation of hydrogen sulfide and aldehydes.
Asunto(s)
Leche/química , Animales , Manipulación de Alimentos , Presión , Compuestos de Azufre/química , Temperatura , VolatilizaciónRESUMEN
Chitosan (Chi), a protein recovery agent for the treatment of aqueous food processing streams, appears to work by mechanical entrapment and electrostatic interaction of chitosan amino groups with anionic groups on proteins. Chitosan effectiveness for recovering soluble proteins from surimi wash water (SWW) is increased by complexation with alginate (Alg) and by adjusting complex concentration and treatment time. Flocculation at 20 degrees C with Chi-Alg at a 0.2 mixing ratio added as 20, 40, 100 and 150 mg/L SWW was aided by 5 min agitation at 130 rpm and then held at the same temperature for 30 min, 1 and 24 h. Turbidity measurements, protein determinations and qualitative FTIR analysis confirmed SWW protein adsorption which depended on Chi-Alg concentration and reaction time while turbidity reduction was affected by concentration only. No differences (p < 0.05) in protein adsorption were found between 1 and 24 h. Using 100 mg Chi-Alg complex/L SWW for 1 h achieved 83% protein adsorption and 97% turbidity reduction.