[A case of acute purulent otitis media complicated by atypical mastoiditis on the background of an unverified HIV infection]. / Sluchai ostrogo gnoinogo srednego otita, oslozhnennogo atipichnym mastoiditom, na fone neverifitsirovannoi VICh-infektsii.

According to the literature, acute otitis media is complicated by mastoiditis in 0.15-1% of cases. In turn, mastoiditis can be complicated by meningitis, encephalitis, abscess of temporal lobe of brain and cerebellum, epidural and subdural abscesses, facial nerve paresis, labyrinthitis, phlegmon of soft tissues of neck, as well as subperiosteal abscess, which makes 7% in the structure of mastoiditis complications. Nowadays, when doctors have a wide range of antibacterial preparations at their disposal, a complicated course of acute otitis media and further mastoiditis is caused both by an aggressive atypical infectious agent and immunocompromised status of a patient. The article deals with a clinical case of a prolonged course of acute otitis media complicated by mastoiditis and subperiosteal abscess against the background of outpatient courses of antibacterial therapy. The examination revealed an atypical pathogen of otitis media Pseudomonas aeruginosa and HIV-positive status of the patient, previously unknown. Timely surgical intervention and the right combination of antibacterial drugs, meropenem and ciprofloxacin, prevented the development of intracranial and septic complications, despite the presence of multiple foci of bone destruction of the mastoid process and temporal bone pyramid, bordering the middle fossa and sigmoid sinus, according to multispiral head computed tomography. As a part of additional examination in the Center for AIDS and Infectious Diseases Prevention and Control, the patient was diagnosed with HIV infection, clinical stage 4C, progressing phase on the background of absence of antiretroviral therapy, and the necessary amount of treatment was prescribed.

[Characteristic of segments of transgenic animal genomes, adjacent to integrated sequences of foreign DNA]. / Kharakteristika uchastkov genoma transgennykh zhivotnykh, prelezhashchikh k integrirovannym posledovatel'nostiam chuzherodnoi DNK.

DNAs of seven transgenic mice and one transgenic rabbit was divided into fractions according to reassociation kinetics and GC-content. Moderate and/or frequent (reverse) repeated sequences of the genome were revealed in all cases next to different transgenes. DNA fractions containing foreign sequences differed by the GC-content in different transgenic animals.

[New data on the type of structural changes in DNA of simian adenovirus SA7 microinjected into mouse zygotes]. / Novye dannye o kharaktere strukturnykh izmenenii DNK adenovirusa obez'ian SA7, mikroin''etsirovannoi v zigoty myshei.

The structure of the transgene has been analysed in a new series of experiments on the transfer of adenovirus SA7 DNA into the mice zygotes by microinjection technique. The previous data on SA7 DNA elimination from the genomes of different organs (sceletal muscles, heart, tail) have been confirmed and detailed for the F0 and F1 generations of transgenic animals. The left end of adenoviral genome has been shown to be predominantly transfered after microinjections of SA7 DNA into the mice zygotes.

[Production of transgenic rabbits and mice carrying the gene for bovine growth hormone]. / Poluchenie transgennykh krolikov i myshei, soderzhashchikh gen gormona rosta byka.

The results of experiments on the transfer of bovine gene for growth hormone into mice and rabbits are presented. The gene was transferred by the technique of microinjection into the zygote. In all cases transgene in rabbits occurred to be changed. In two transgenic mice the bovine growth hormone gene represented some tandem arranged copies. One of the mice had accelerated growth. This phenotypic changes is found to be inheritable.

Microinjection of simian adenovirus SA7 (C-8) DNA into the mouse zygotes: differential distribution of viral DNA in organs.

A simian adenovirus SA7 (C-8) DNA was microinjected into fertilized mouse eggs. Thirty-five mice derived from eggs injected with SA7 DNA were screened for the presence of the adenovirus genome in their liver DNA. Eighteen of these mice contained the virus-specific sequences. SA7 DNA was detected in some tissues, but in all cases, viral sequences were absent from muscle and heart DNA. Viral DNA was inherited by 50-70% of the next generation. One mouse that contained about 1 copy of SA7 DNA per haploid genome has been shown to pass it on to five generations, although the integrated viral DNA sustained a considerable structural change between F1 and F3. RNA analysis in various organs of 12 mice has shown the transcription of SA7 DNA to be very infrequent: only in the kidney of one mouse and in the spleen of another did RNA contain SA7-specific sequences.

[Size and organization of repetitive sequences in pigeon genome]. / Razmer i organizatsiia povtoriaiushchikhsia posledovatel'nostei v genome golubia.

Organization of sequences in pigeon genome and the spectrum of their repetition frequencies were studied by means of DNA/DNA reassociation. Reassociation of 125I-labelled DNA fractions isolated from pigeon total DNA attested the presence of rare repetitions with an average of 50 copies within a gaploid genome. The disposition of repetitive and unique sequences was studied by reassociation of the labelled fragments of different length with an essential excess of short fragments of an unlabelled DNA. Additional evidence was provided by estimation of hyperchromicity and resistance to nuclease S1 of long DNA fragments, reassociated to the given C0t values. It was demonstrated that approximately one fourth of the pigeon genome consists of intermittent repetitive and unique sequences with individual elements of average length of 2 and 37 kb, respectively (1 kb = = 1000 nucleotide base pairs). A hypothetical organization of palindromic sequences in pigeon genome is discussed in terms of the dependence of the value of zero binding to hydroxyapatite on the fragment length.

[Metabolically stable classes of messenger-like nuclear RNA. II. Existence of homology between the 28S fraction of nuclear RNA, giant non-polyadenylated molecules of heterogeneous nuclear RNA and polyadenylated molecules of cytoplasmic RNA]. / Metabolicheski stabil'nye klassy iadernoi RNK informatsionnogo tipa. II. Nalichie gomologii mezhdu 28S fraktsiei iadernoi RNK, gigantskimi poli (A-)-molekulami geterogennoi iadernoi RNK i poli (A+)-molekulami tsitoplazmaticheskoi RNK.

The existence and extent of homology between the 28S fraction of messenger-like nuclear RNA on one hand, and giant (greater than 45S) molecules of heterogeneous nuclear RNA and various fractions of cytoplasmic RNA on the other hand were studied by the method of competitive hybridization. The results obtained lead to the conclusion that the poly(A)-subfraction of hnRNA molecules with the size of greater than 15 000 nucleotides contains molecules subjected to rapid but incomplete processing with the formation of chains approximately 5000 nucleotides long (approximately 28S), which are then stored in the nucleus for up to 30 hours. Later polyadenylated and non-polyadenylated molecules of cytoplasmic RNA with the size of less than or equal to 2000 nucleotides are formed from the stored precursors.

[Metabolically stable classes of nuclear messenger-like RNA. I. Detection and molecular characterization]. / Metabolicheski stabil'nye kalssy iadernoi RNK informatsionnogo tipa. I. Vyiavlenie i molekuliarnaia kharakteristika.

Two discreet in size molecular classes of metabolically stable messenger-like RNA molecules with sedimentation coefficientes about 28S and 18S have been revealed in the nuclei of pigeon bone marrow cells. The structural pecularities of 28S RNA class were investigated more carefully. It was shown to constitute the largest fraction of nuclear messenger-like RNA and is characterized by a GC/AU ratio 1.13. As pulse-labeled nuclear RNA, 28S stable nuclear RNA hybridizes with the unique and rare repeated DNA, it does not contain poly(A)-sequences and is found in a form of RNP particles with density of 1.41 g/sm3, tightly bound to chromatin.

[Isolation and characteristics of 2 transcribing families of pigeon-genome repetitive sequences]. / Vydelenie i kharakteristika dvukh transkribiruiushchikhsia semeistv povtoriaiushchikhsia posledovatel'nostei genoma golubia.

From pigeon DNA two families of repeats have been isolated: one (frequency of repetitions 30--40 times per haploid genome) actively transcribing and the second (frequency of repetitions 2000--2800 times) weakly transcribing in erythroid cells. The reassociation kinetics, GC-content and size of isolated repeats were investigated. Actively transcribing repeats (average length of 350 nucleotides) contain about 55% GC-nucleotides and alternate in the genome with AT-rich sequences of the unique type. Comparison of isolated families of repeats revealed differences in the structure and posttranscriptional fate of RNA transcribed from them.

Purification and characterization of two transcribed repetitive DNA fractions from the pigeon genome.

Two fractions of the repeats belonging to intermediate frequency repetitive DNA were isolated from the total pigeon nuclear DNA fragmented to about 450 nucleotides. One fraction was designated as "rare repeats" (repetition frequency about 35 per haploid genome) and another termed as "moderate repeats" (repetition frequency about 2500 per haploid genome). The rare repeats, which constitute about 7% of the total DNA, include at least 75% of the repetitive DNA sequences transcribed into the high molecular fraction (greater than 45S) of HnRNA in erythroid cells. These repeats have properties compatible with the characteristics of the class of low frequency interspersed DNA found in genomes of many other Metazoan species. The moderate repeats contribute only about 10-20% of the total repetitive DNA copies present in greater than 45S HnRNA and differ from the rare repeats in some other properties.--The possible role of the rare repeats in the genome is discussed.