ABSTRACT
AIM: To examine the association between treatment-achieved HbA1c values and incidence of both coronary artery disease (CAD) and severe eye disease with different diabetes treatments. METHODS: Associations of treatment-achieved HbA1c were investigated in various treatment groups [diet only; insulin; sulphonylurea (SU) alone; SU with glinides; and antihyperglycaemic agents other than glinides, SU or insulin] taken from a nationwide claims database of 14,633 Japanese diabetes patients. Cox's regression analysis examined risks over a 5.1-year follow-up. RESULTS: A significant linear trend was associated with HbA1c levels and CAD events in the diet-only group, and CAD risks were significantly higher in insulin and SU groups with HbA1c ≤ 7.0% and > 8.0% than in the diet-only group with HbA1c ≤ 7.0%. In contrast to CAD, a linear association was observed regardless of treatment modality between achieved HbA1c levels and risk of severe diabetic eye disease, but with no significant difference in eye disease risk between groups with HbA1c ≤ 7.0% and 7.1-8.0% in those treated with either SU alone, SU with glinides, or insulin. CONCLUSION: These findings suggest that the relationship between treatment-achieved HbA1c and incidence of both CAD and severe diabetic eye disease differed according to treatment, based on a large-scale real-life database. More research is now needed to confirm these findings and to further investigate the underlying mechanisms.
Subject(s)
Coronary Artery Disease/epidemiology , Diabetes Mellitus, Type 2/therapy , Diabetic Retinopathy/epidemiology , Diet, Diabetic , Glycated Hemoglobin/metabolism , Hypoglycemic Agents/therapeutic use , Macular Edema/epidemiology , Angiogenesis Inhibitors/therapeutic use , Diabetes Mellitus, Type 2/metabolism , Diabetic Retinopathy/therapy , Female , Humans , Incidence , Insulin/therapeutic use , Intravitreal Injections , Light Coagulation , Macular Edema/physiopathology , Macular Edema/therapy , Male , Middle Aged , Proportional Hazards Models , Severity of Illness Index , Sulfonylurea Compounds/therapeutic use , Treatment OutcomeABSTRACT
AIM: Primary aldosteronism, which is usually caused by an aldosterone-producing tumour, affects glucose metabolism. The effects of this condition on insulin secretion and insulin sensitivity have remained unclear, however. To gain insight into the influence of primary aldosteronism on glucose tolerance, various parameters related to insulin secretion or insulin sensitivity in patients with an aldosterone-producing tumour were comprehensively analyzed. METHODS: To assess 14 patients with an aldosterone-producing tumour, hyperglycaemic and hyperinsulinaemic-euglycaemic clamp tests as well as oral glucose tolerance tests (OGTTs) were performed before and after tumour excision. Time between presurgical analysis and surgery was 27-559 (194±132) days, and 14-142 (51±38) days between surgery and postsurgical analysis. Various parameters related to insulin secretion or sensitivity as determined by OGTT as well as hyperglycaemic and hyperinsulinaemic-euglycaemic clamp analyses were evaluated. RESULTS: Surgical treatment of tumours ameliorated hypokalaemia and reduced plasma aldosterone levels. First and second phases of insulin secretion during the hyperglycaemic clamp, as well as the insulinogenic index and total insulin secretion measured during OGTT, were also improved after surgery. In addition, the insulin sensitivity index determined during the hyperinsulinaemic-euglycaemic clamp was reduced after surgery. CONCLUSION: Primary aldosteronism impairs insulin secretion.
Subject(s)
Adrenal Cortex Neoplasms/surgery , Adrenocortical Adenoma/surgery , Aldosterone/blood , Hyperaldosteronism/surgery , Insulin Resistance/physiology , Insulin Secretion/physiology , Insulin/blood , Adrenal Cortex Neoplasms/blood , Adrenal Cortex Neoplasms/complications , Adrenocortical Adenoma/blood , Adrenocortical Adenoma/complications , Adult , Aged , Blood Glucose/analysis , Female , Glucose Clamp Technique , Glucose Tolerance Test , Glycated Hemoglobin/analysis , Humans , Hyperaldosteronism/blood , Hyperaldosteronism/etiology , Male , Middle AgedABSTRACT
AIMS: To verify the effect of awards after dental care in children's motivation in two visits to the dentist and if differences occur between genders. METHODS: This was a randomised and blinded study with a systematic convenience sample consisting of 306 children of 4.99 ± 0.89 years old, with no previous dental experience for both genders, who sought the public dental services. The children were divided into Control group (G1) and Experimental group (G2), who received a positive reinforcement technique with awards after dental care. A projective test with the Venham Picture Test self-analysis was applied and the inference test was the Chi square test with a significance level of p < 0.05. RESULTS: In G1, there was evidence of a significant association between the T1, T2 and T4 application times and anxiety levels measured in children (p < 10- 4; x2 = 15.43), this same association was observed for the G2 group (p < 10- 10; x2 > 29.46). For boys there was no significant difference of anxiety between G1 and G2 groups, however in G2, girls showed more anxiety before dental treatment (p = 0.0095; x2 = 6.71) and less anxiety than boys during the second visit (p = 0.0014; x2 = 10.20). CONCLUSIONS: The award after dental care demonstrated a positive result for the decrease of anxiety in preschool children for two visits to the dentist. Girls in the experimental group showed less anxiety than boys during the second visit.
Subject(s)
Dental Anxiety , Dental Care for Children , Reinforcement, Psychology , Child , Child Behavior , Child, Preschool , Dental Care , Female , Humans , Male , MotivationABSTRACT
The rat enhancer of split- and hairy-related protein-1 (SHARP-1) is a basic helix-loop-helix transcription factor. An issue of whether SHARP-1 is an insulin-inducible transcription factor was examined. Insulin rapidly increased the level of SHARP-1 mRNA both in vivo and in vitro. Then, signaling pathways involved with the increase of SHARP-1 mRNA by insulin were determined in H4IIE rat hepatoma cells. Pretreatments with LY294002, wortmannin, and staurosporine completely blocked the induction effect, suggesting the involvement of both phosphoinositide 3-kinase (PI 3-K) and protein kinase C (PKC) pathways. In fact, overexpression of a dominant negative form of atypical protein kinase C lambda (aPKCλ) significantly decreased the induction of the SHARP-1 mRNA. In addition, inhibitors for the small GTPase Rac or Jun N-terminal kinase (JNK) also blocked the induction of SHARP-1 mRNA by insulin. Overexpression of a dominant negative form of Rac1 prevented the activation by insulin. Furthermore, actinomycin D and cycloheximide completely blocked the induction of SHARP-1 mRNA by insulin. Finally, when a SHARP-1 expression plasmid was transiently transfected with various reporter plasmids into H4IIE cells, the promoter activity of PEPCK reporter plasmid was specifically decreased. Thus, we conclude that insulin induces the SHARP-1 gene expression at the transcription level via a both PI 3-K/aPKCλ/JNK- and a PI 3-K/Rac/JNK-signaling pathway; protein synthesis is required for this induction; and that SHARP-1 is a potential repressor of the PEPCK gene expression.
Subject(s)
Basic Helix-Loop-Helix Transcription Factors/genetics , Gene Expression Regulation/drug effects , Insulin/pharmacology , Signal Transduction/genetics , Animals , Basic Helix-Loop-Helix Transcription Factors/metabolism , Humans , Intracellular Signaling Peptides and Proteins/genetics , Isoenzymes/metabolism , Male , Phosphoenolpyruvate Carboxykinase (GTP)/genetics , Promoter Regions, Genetic/genetics , Protein Biosynthesis/drug effects , Protein Kinase C/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats, Sprague-Dawley , Signal Transduction/drug effects , Transcription, Genetic/drug effects , rac1 GTP-Binding Protein/metabolismABSTRACT
Moderate-intensity exercise at the lactate threshold (LT) is considered to be a safe and effective training regimen for improving metabolic syndrome. The aim of the current study was to investigate the effects of moderate exercise performed at the LT on skeletal muscle gene expression. 6 healthy men participated in cycle ergometer training at LT, 60 min/d, 5 d/wk for 12 wks. Muscle samples were collected after 5 d of training, and then 2 d after training at wks 6 and 12. Quantitative real-time PCR analysis revealed that the expression of peroxisome proliferator activated receptor co-activated 1alpha was significantly increased at 1 h after the training session on day 5. Moreover, using serial analysis gene expression, we found that moderate training for 6 and 12 wks simultaneously induced the expression of a number of metabolic genes involved in the TCA cycle, beta-oxidation, and electron transport. Furthermore, several genes encoding antioxidant enzymes and contractile apparatus were induced. The expression levels of 233 novel transcripts were also altered in response to moderate exercise. Thus, moderate training at the LT is a sufficient stimulus to induce the expression of numerous genes implicated in the development of metabolic syndrome, transcripts involved in the contractile apparatus, and novel transcripts.
Subject(s)
Bicycling/physiology , Gene Expression Regulation/physiology , Muscle, Skeletal/physiology , Adult , Anaerobic Threshold/physiology , Ergometry , Exercise/physiology , Humans , Lactic Acid/blood , Male , Polymerase Chain Reaction , Young AdultABSTRACT
The nucleus tractus solitarius (NTS) plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs) in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 microM DL-2-amino-5-phosphonopentanoic acid. The I-V relationship of the NMDA response shows that current, with a mean (+/- SEM) amplitude of -41.2 +/- 5.5 pA, is present even at a holding potential of -60 mV, suggesting that the NMDA receptors are weakly blocked by extracellular Mg2+ at near resting membrane potentials. This weak block can also be inferred from the value of 0.67 +/- 0.17 for parameter delta obtained from a fit of the Woodhull equation to the I-V relationship. The maximal inward current measured on the I-V relationship was at -38.7 +/- 4.2 mV. The decay phase of the NMDA currents was fitted with one exponential function with a decay time constant of 239 +/- 51 and 418 +/- 80 ms at a holding potential of -60 and +50 mV, respectively, which became slower with depolarization (e-fold per 145 mV). The biophysical properties of the NMDA receptors observed in the present study suggest that these receptors in the NTS contain NR2C subunits and may contribute to the synaptic signal integration.
Subject(s)
Neurons/chemistry , Receptors, N-Methyl-D-Aspartate/analysis , Solitary Nucleus/chemistry , Synapses/physiology , Synaptic Transmission/physiology , Animals , Electrophysiology , Female , Male , Membrane Potentials/physiology , Neurons/physiology , Patch-Clamp Techniques , Rats , Rats, Wistar , Solitary Nucleus/physiologyABSTRACT
Whole-cell patch clamp recordings were made from neurons of the rat subpostremal region of the nucleus tractus solitarius (NTS) in transverse brainstem slices. Neurotensin (NT) enhanced the firing rate of action potentials from 0.8 +/- 0.4 Hz in control to 1.9 +/- 1.3 Hz (n = 9) and increased their decay time. The peak amplitude of the after-hyperpolarization was decreased by 34+/-5% (n = 9). These effects were associated with a depolarization of 4 +/- 1 mV (n = 10) in the resting membrane potential and an increase in the input resistance (from 768 +/- 220 MOmega to 986+/-220 MOmega; n = 5) and were compensated by manually hyperpolarizing the cell to control values. In voltage clamp experiments NT decreased an outward current (from 488 +/- 161 to 340 +/- 96 pA at +40 mV; n = 5) which reversed near the potassium equilibrium potential. In addition, NT increased the frequency of both excitatory and inhibitory spontaneous synaptic currents, an effect blocked by tetrodotoxin, and did not change the evoked excitatory or inhibitory postsynaptic currents. The selective NTR1 receptor antagonist SR48692 reversibly blocked the effects of NT on both action potentials and spontaneous synaptic currents. Our results suggest that NTR1 receptors can modulate post-synaptic responses in neurons of the subpostremal NTS by increasing cell excitability as a result of blockade of a potassium conductance.
Subject(s)
Neurotensin/metabolism , Presynaptic Terminals/metabolism , Solitary Nucleus/metabolism , Synaptic Transmission/physiology , Action Potentials/drug effects , Action Potentials/physiology , Animals , Female , Male , Neural Inhibition/drug effects , Neural Inhibition/physiology , Neurotensin/pharmacology , Organ Culture Techniques , Patch-Clamp Techniques , Potassium Channels/drug effects , Potassium Channels/metabolism , Presynaptic Terminals/drug effects , Pyrazoles/pharmacology , Quinolines/pharmacology , Rats , Rats, Wistar , Receptors, Neurotensin/antagonists & inhibitors , Receptors, Neurotensin/metabolism , Solitary Nucleus/drug effects , Synaptic Transmission/drug effectsABSTRACT
The nucleus tractus solitarius (NTS) plays an important role in the control of autonomic reflex functions. Glutamate, acting on N-methyl-D-aspartate (NMDA) and non-NMDA ionotropic receptors, is the major neurotransmitter in this nucleus, and the relative contribution of each receptor to signal transmission is unclear. We have examined NMDA excitatory postsynaptic currents (NMDA-EPSCs) in the subpostremal NTS using the whole cell patch clamp technique on a transverse brainstem slice preparation. The NMDA-EPSCs were evoked by stimulation of the solitary tract over a range of membrane potentials. The NMDA-EPSCs, isolated pharmacologically, presented the characteristic outward rectification and were completely blocked by 50 æM DL-2-amino-5-phosphonopentanoic acid. The I-V relationship of the NMDA response shows that current, with a mean (± SEM) amplitude of -41.2 ± 5.5 pA, is present even at a holding potential of -60 mV, suggesting that the NMDA receptors are weakly blocked by extracellular Mg2+ at near resting membrane potentials. This weak block can also be inferred from the value of 0.67 ± 0.17 for parameter delta obtained from a fit of the Woodhull equation to the I-V relationship. The maximal inward current measured on the I-V relationship was at -38.7 ± 4.2 mV. The decay phase of the NMDA currents was fitted with one exponential function with a decay time constant of 239 ± 51 and 418 ± 80 ms at a holding potential of -60 and +50 mV, respectively, which became slower with depolarization (e-fold per 145 mV). The biophysical properties of the NMDA receptors observed in the present study suggest that these receptors in the NTS contain NR2C subunits and may contribute to the synaptic signal integration.
Subject(s)
Animals , Male , Female , Rats , Neurons/chemistry , Receptors, N-Methyl-D-Aspartate/analysis , Solitary Nucleus/cytology , Synapses/physiology , Synaptic Transmission/physiology , Electrophysiology , Membrane Potentials/physiology , Neurons/physiology , Patch-Clamp Techniques , Rats, Wistar , Solitary Nucleus/physiologyABSTRACT
Vascular diseases such as atherosclerosis are characterized by abnormal accumulation of vascular smooth muscle cells (VSMCs) within the intimal lining. The intimal VSMCs exhibit an increased expression of peroxisome proliferator-activated receptor gamma (PPARgamma), and the administration of pharmacological PPARgamma agonists attenuates vascular lesion formation. The factors that regulate PPARgamma expression in the vasculature are poorly defined. Here we report that platelet-derived growth factor (PDGF) upregulates PPARgamma by the phosphatidylinositol 3-kinase (PI3-kinase)/Akt signaling pathway. Using Northern-blotting and Western-blotting analyses, we observed that the levels of PPARgamma mRNA and protein were increased by 2- to 3.5-fold in human aortic smooth muscle cells (HASMCs) treated with PDGF (20 ng/mL). This was abolished by preincubation of HASMCs with a PI3-kinase inhibitor (LY294002, 50 micromol/L), and partially inhibited by a MEK1 inhibitor (U0126, 10 micromol/L), but not affected by a p38 kinase inhibitor (SB202190, 10 micromol/L). In addition, overexpression of the dominant-negative p85 subunit of PI3-kinase or Akt proteins blocked the PDGF-induced PPARgamma expression. Taken together, our results suggest that PDGF induces PPARgamma expression in VSMCs by a PI3-kinase/Akt signaling pathway. The characterization of factors and signaling pathways that modulate PPARgamma expression in VSMCs may have important implications for understanding the pathogenesis of vascular diseases.
Subject(s)
Muscle, Smooth, Vascular/metabolism , Phosphatidylinositol 3-Kinases/physiology , Platelet-Derived Growth Factor/pharmacology , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/physiology , Receptors, Cytoplasmic and Nuclear/biosynthesis , Signal Transduction , Transcription Factors/biosynthesis , Aorta/cytology , Cells, Cultured , Dose-Response Relationship, Drug , Humans , Kinetics , Muscle, Smooth, Vascular/drug effects , Proto-Oncogene Proteins c-akt , RNA Stability/drug effects , RNA, Messenger/biosynthesis , Receptors, Cytoplasmic and Nuclear/genetics , Transcription Factors/genetics , Transcriptional ActivationABSTRACT
Evidence has suggested that the insulin receptor substarate(IRS)-phosphoinositide 3-kinase(PI3K) pathway plays a central role in metabolic actions of insulin. The downstream effectors that mediate PI 3-kinase-dependent actions, such as Akt or atypical PKC, have been identified. Lipid and protein phosphatases that modulate the IRS-PI3 K pathway are implicated in the development of insulin resistance. The abundance of IRS, regulated at the level of both mRNA and protein, may also contribute to the sensitivity of cells to insulin. Tissue-specific insulin receptor knockout mice involved in insulin signaling are disrupted have reveled the impact of the defects of insulin signaling in a specific tissue.
Subject(s)
Insulin/physiology , Muscle Proteins , Signal Transduction , Animals , CCAAT-Enhancer-Binding Proteins/genetics , CCAAT-Enhancer-Binding Proteins/physiology , Cell Membrane/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/physiology , Energy Metabolism , Forkhead Box Protein O1 , Forkhead Transcription Factors , GTP-Binding Proteins/physiology , Glucose Transporter Type 4 , Humans , Insulin Resistance , Mice , Monosaccharide Transport Proteins/metabolism , Phosphatidylinositol 3-Kinases/physiology , Receptor, Insulin/physiology , Sterol Regulatory Element Binding Protein 1 , Transcription Factors/physiology , Transcription, GeneticABSTRACT
Insulin inhibits expression of certain liver genes through the phosphoinositol (PI) 3-kinase/Akt pathway. However, whether Akt activity is both necessary and sufficient to mediate these effects remains controversial. The forkhead proteins (Foxo1, Foxo3, and Foxo4, previously known as Fkhr or Afx) are transcriptional enhancers, the activity of which is inhibited by insulin through phosphorylation-dependent translocation and nuclear exclusion. Others and we have previously shown that the forkhead protein Foxo1 is phosphorylated at three different sites: S(253), T(24), and S(316). We have also shown that T(24) fails to be phosphorylated in hepatocytes lacking insulin receptors, and we have suggested that this residue is targeted by a kinase distinct from Akt. In this study, we have further analyzed the ability of Akt to phosphorylate different Foxo1 sites in control and insulin receptor-deficient hepatocytes. Expression of a dominant negative Akt (Akt-AA) in control hepatocytes led to complete inhibition of endogenous Akt, but failed to inhibit Foxo1 T(24) phosphorylation and, consequently, insulin suppression of IGFBP-1 promoter activity. Conversely, expression of a constitutively active Akt (Akt-Myr) in insulin receptor-deficient hepatocytes led to an overall increase in the level of Foxo1 phosphorylation, but failed to induce T(24) and S(316) phosphorylation. These data indicate that the Foxo1 T(24) and S(316) kinases are distinct from Akt, and suggest that the pathways required for insulin regulation of hepatic gene expression diverge downstream of PI 3-kinase.
Subject(s)
DNA-Binding Proteins/physiology , Gene Expression Regulation/drug effects , Insulin/pharmacology , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/physiology , Transcription Factors/physiology , Animals , Antibodies/immunology , Antibody Specificity , Cell Line, Transformed , DNA-Binding Proteins/immunology , Forkhead Box Protein O1 , Forkhead Transcription Factors , Hepatocytes/drug effects , Hepatocytes/metabolism , Insulin-Like Growth Factor Binding Protein 1/genetics , Insulin-Like Growth Factor I/pharmacology , Mice , Phosphorylation , Promoter Regions, Genetic , Proto-Oncogene Proteins c-akt , Subcellular Fractions/enzymology , Transcription Factors/immunologyABSTRACT
BACKGROUND: The novel serine-threonine kinase Akt is a critical enzyme in cell survival. We investigated the roles of the Akt pathway and apoptotic signals in (1) Madin-Darby canine kidney (MDCK) cells in a hyperosmotic condition in vitro and (2) in the inner medulla of dehydrated rat in vivo. METHODS: The in vivo experiments were performed in 24- and 48-hour water-restricted rats. Hyperosmolality-stimulated Akt phosphorylation was examined in MDCK cells. Phosphatidylinositol 3-kinase (PI3-K) inhibitors, the dominant-negative mutant of PI3-K, the dominant-negative mutant of Akt, and the dominant-active form of Akt were used to examine the roles of the PI3-K/Akt pathways in renal tubular cell apoptosis. RESULTS: The amount of phosphorylated Akt protein was increased in the inner medulla of dehydrated rats. Hyperosmolality induced by the addition of NaCl, urea, and raffinose phosphorylated Akt in MDCK cells in an osmolality-dependent manner. PI3-K inhibitors and the dominant-negative mutant of PI3-K inhibited the hyperosmolality-induced phosphorylation of Akt. Raising the media osmolality from a normal level to 500 or 600 mOsm/kg H2O final osmolality elicited apoptotic changes such as nucleosomal laddering of DNA and an increment of caspase-3 activity and increased activity in the cell death enzyme-linked immunosorbent assay. Dominant-active Akt prevented the mild hyperosmolality-induced apoptosis, while inhibition of the PI3-K/Akt pathways promoted apoptosis. CONCLUSION: The Akt pathway is activated by hyperosmolality in vitro and in vivo, and activation of Akt prevents the mild hyperosmolality-induced apoptotic changes in MDCK cells. PI3-K/Akt pathways are involved in a hypertonic condition that confers the balance between cell survival and apoptosis.
Subject(s)
Apoptosis/physiology , Dehydration/metabolism , Kidney Tubules/metabolism , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/metabolism , Water-Electrolyte Balance/physiology , Androstadienes/pharmacology , Animals , Caspase 3 , Caspases/metabolism , Chromones/pharmacology , Enzyme Inhibitors/pharmacology , Kidney Medulla/cytology , Kidney Medulla/metabolism , Kidney Tubules/cytology , LLC-PK1 Cells , Male , Morpholines/pharmacology , Mutagenesis/physiology , Osmotic Pressure , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositol 3-Kinases/metabolism , Phosphoinositide-3 Kinase Inhibitors , Phosphorylation , Proto-Oncogene Proteins c-akt , Rats , Rats, Sprague-Dawley , Swine , Transfection , Water Deprivation/physiology , WortmanninABSTRACT
AIM/HYPOTHESIS: We previously reported that repeated subcutaneous or intracerebroventricular injection of brain-derived neurotrophic factor (BDNF) reduces blood glucose concentrations in obese diabetic C57BL/KsJ-db/db mice. In this study, we assessed the effects of BDNF on insulin action in peripheral tissues of diabetic mice. METHODS: First, brain-derived neurotrophic factor (20 mg/kg) was subcutaneously given to male db/db mice for 14 days and then the insulin-stimulated tyrosine phosphorylation of insulin receptors and insulin-stimulated phosphatidylinositol (PI) 3-kinase activity in peripheral tissues was assessed. Second, we examined the effects of a single subcutaneous or intracerebroventricular brain-derived neurotrophic factor injection on insulin responsiveness in liver and skeletal muscle of streptozotocin (STZ)-induced diabetic mice. Third, the effects of brain-derived neurothrophic factor on insulin action were also examined in cultured cells. RESULTS: Repeated injection of BDNF to db/db mice for 14 days enhanced insulin-stimulated tyrosine phosphorylation of insulin receptors in liver and insulin-stimulated PI 3-kinase activity in liver, skeletal muscle and interscapular brown adipose tissue. We then examined the rapid effect of BDNF on insulin signalling in vivo. A single subcutaneous or intracerebroventricular injection of BDNF rapidly increased insulin-stimulated tyrosine phosphorylation of insulin receptors and PI 3-kinase activity in liver of STZ-mice. No direct effect of brain-derived neurothrophic factor was observed on insulin signalling in primary cultured hepatocytes, L6 muscle cells or 3T3-L1 adipocytes. Brain-derived neurothrophic factor did not affect either glucose uptake or gluconeogenesis in these cells. CONCLUSION/INTERPRETATION: These data indicate that brain-derived neurothrophic factor rapidly enhances insulin signal transduction in liver and shows hypoglycaemic action in diabetic mice.
Subject(s)
Brain-Derived Neurotrophic Factor/pharmacology , Diabetes Mellitus, Experimental/physiopathology , Insulin/pharmacology , Liver/metabolism , Receptor, Insulin/metabolism , 3T3 Cells , Adipose Tissue, Brown/drug effects , Adipose Tissue, Brown/metabolism , Animals , Blood Glucose/drug effects , Blood Glucose/metabolism , Brain-Derived Neurotrophic Factor/administration & dosage , Cell Line , Cerebral Ventricles/drug effects , Gluconeogenesis/drug effects , Glucose/metabolism , Hepatocytes/drug effects , Hepatocytes/physiology , Humans , Injections, Intraventricular , Insulin/physiology , Kinetics , Liver/drug effects , Male , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Rats , Rats, Wistar , Receptor, Insulin/drug effects , Recombinant Proteins/administration & dosage , Recombinant Proteins/pharmacology , Signal Transduction/drug effects , Signal Transduction/physiologyABSTRACT
The activation of phosphatidylinositol (PI) 3-kinase and Akt/protein kinase B (PKB) by tumor necrosis factor (TNF)-alpha and their roles on stimulation of protein synthesis were investigated in cultured neonatal rat cardiac myocytes. Treatment of cells with TNF-alpha resulted in enlargement of cell surface area and stimulation of protein synthesis without affecting myocyte viability. TNF-alpha induced marked activation of PI3-kinase and Akt/PKB, and the activation of PI3-kinase and Akt/PKB was rapid (maximal at 10 and 15 min, respectively) and concentration dependent. Akt/PKB activation by TNF-alpha was inhibited by a PI3-kinase-specific inhibitor LY-294002 and adenovirus-mediated expression of a dominant negative mutant of PI3-kinase, indicating that TNF-alpha activates Akt/PKB through PI3-kinase activation. Furthermore, TNF-alpha-induced protein synthesis was inhibited by pretreatment with LY-294002 and expression of a dominant negative mutant of PI3-kinase or Akt/PKB. These results indicate that activation of the PI3-kinase-Akt/PKB pathway plays an essential role in protein synthesis induced by TNF-alpha in cardiac myocytes.
Subject(s)
Myocardium/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Protein Serine-Threonine Kinases , Proto-Oncogene Proteins/metabolism , Signal Transduction/physiology , Tumor Necrosis Factor-alpha/pharmacology , Animals , Cell Membrane/drug effects , Cell Membrane/metabolism , Cell Survival , Cells, Cultured , Chromones/pharmacology , Enzyme Activation , Enzyme Inhibitors/pharmacology , Histones/metabolism , Kinetics , Morpholines/pharmacology , Myocardium/cytology , Phosphatidylinositol 3-Kinases/genetics , Phosphotyrosine/metabolism , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins c-akt , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects , Transfection , beta-Galactosidase/biosynthesis , beta-Galactosidase/geneticsABSTRACT
Phosphatidylinositol (PI) 3-kinase plays an important role in various metabolic actions of insulin including glucose uptake and glycogen synthesis. Although PI 3-kinase primarily functions as a lipid kinase which preferentially phosphorylates the D-3 position of phospholipids, the effect of hydrolysis of the key PI 3-kinase product PI 3,4,5-triphosphate [PI(3,4,5)P3] on these biological responses is unknown. We recently cloned rat SH2-containing inositol phosphatase 2 (SHIP2) cDNA which possesses the 5'-phosphatase activity to hydrolyze PI(3,4,5)P3 to PI 3,4-bisphosphate [PI(3,4)P2] and which is mainly expressed in the target tissues of insulin. To study the role of SHIP2 in insulin signaling, wild-type SHIP2 (WT-SHIP2) and 5'-phosphatase-defective SHIP2 (Delta IP-SHIP2) were overexpressed in 3T3-L1 adipocytes by means of adenovirus-mediated gene transfer. Early events of insulin signaling including insulin-induced tyrosine phosphorylation of the insulin receptor beta subunit and IRS-1, IRS-1 association with the p85 subunit, and PI 3-kinase activity were not affected by expression of either WT-SHIP2 or Delta IP-SHIP2. Because WT-SHIP2 possesses the 5'-phosphatase catalytic region, its overexpression marked by decreased insulin-induced PI(3,4,5)P3 production, as expected. In contrast, the amount of PI(3,4,5)P3 was increased by the expression of Delta IP-SHIP2, indicating that Delta IP-SHIP2 functions in a dominant-negative manner in 3T3-L1 adipocytes. Both PI(3,4,5)P3 and PI(3,4)P2 were known to possibly activate downstream targets Akt and protein kinase C lambda in vitro. Importantly, expression of WT-SHIP2 inhibited insulin-induced activation of Akt and protein kinase C lambda, whereas these activations were increased by expression of Delta IP-SHIP2 in vivo. Consistent with the regulation of downstream molecules of PI 3-kinase, insulin-induced 2-deoxyglucose uptake and Glut4 translocation were decreased by expression of WT-SHIP2 and increased by expression of Delta IP-SHIP2. In addition, insulin-induced phosphorylation of GSK-3beta and activation of PP1 followed by activation of glycogen synthase and glycogen synthesis were decreased by expression of WT-SHIP2 and increased by the expression of Delta IP-SHIP2. These results indicate that SHIP2 negatively regulates metabolic signaling of insulin via the 5'-phosphatase activity and that PI(3,4,5)P3 rather than PI(3,4)P2 is important for in vivo regulation of insulin-induced activation of downstream molecules of PI 3-kinase leading to glucose uptake and glycogen synthesis.
Subject(s)
Adipocytes/enzymology , Arabidopsis Proteins , Muscle Proteins , Phosphoric Monoester Hydrolases/biosynthesis , Phosphoric Monoester Hydrolases/physiology , 3T3 Cells , Adenoviridae/genetics , Animals , Blotting, Western , Catalysis , Chromatography, High Pressure Liquid , Cloning, Molecular , DNA, Complementary/metabolism , Deoxyglucose/pharmacokinetics , Gene Transfer Techniques , Genes, Dominant , Glucose Transporter Type 1 , Glucose Transporter Type 4 , Glycogen/biosynthesis , Glycogen Synthase/metabolism , Insulin/metabolism , Insulin/pharmacology , Insulin Receptor Substrate Proteins , Isoenzymes , Lac Operon , Mice , Microscopy, Confocal , Models, Genetic , Monosaccharide Transport Proteins/metabolism , Phosphatidylinositol 3-Kinases/metabolism , Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases , Phosphatidylinositols/metabolism , Phosphoproteins/metabolism , Phosphoric Monoester Hydrolases/chemistry , Phosphorylation , Plant Proteins/metabolism , Potassium Channels/metabolism , Precipitin Tests , Protein Kinase C/metabolism , Rats , Signal Transduction , Time Factors , Transfection , src Homology DomainsABSTRACT
Akt, also known as protein kinase B, is a protein-serine/threonine kinase that is activated by growth factors in a phosphoinositide (PI) 3-kinase-dependent manner. Although Akt mediates a variety of biological activities, the mechanisms by which its activity is regulated remain unclear. The potential role of the epsilon isozyme of protein kinase C (PKC) in the activation of Akt induced by insulin has now been examined. Expression of a kinase-deficient mutant of PKCepsilon (epsilonKD), but not that of wild-type PKCepsilon or of kinase-deficient mutants of PKCalpha or PKClambda, with the use of adenovirus-mediated gene transfer inhibited the phosphorylation and activation of Akt induced by insulin in Chinese hamster ovary cells or L6 myotubes. Whereas the epsilonKD mutant did not affect insulin stimulation of PI 3-kinase activity, the phosphorylation and activation of Akt induced by a constitutively active mutant of PI 3-kinase were inhibited by epsilonKD, suggesting that epsilonKD affects insulin signaling downstream of PI 3-kinase. PDK1 (3'-phosphoinositide-dependent kinase 1) is thought to participate in Akt activation. Overexpression of PDK1 with the use of an adenovirus vector induced the phosphorylation and activation of Akt; epsilonKD inhibited, whereas wild-type PKCepsilon had no effect on, these actions of PDK1. These results suggest that epsilonKD inhibits the insulin-induced phosphorylation and activation of Akt by interfering with the ability of PDK1 to phosphorylate Akt.
Subject(s)
Isoenzymes/chemistry , Mutation , Protein Kinase C/metabolism , Proto-Oncogene Proteins/metabolism , 3-Phosphoinositide-Dependent Protein Kinases , Adenoviridae/genetics , Adenoviridae/metabolism , Animals , Binding Sites , CHO Cells , Cell Line , Cricetinae , Enzyme Activation , Insulin/metabolism , Mice , Muscles/cytology , Phosphatidylinositol 3-Kinases/metabolism , Phosphorylation , Protein Binding , Protein Kinase C/chemistry , Protein Kinase C/physiology , Protein Serine-Threonine Kinases/metabolism , Protein Structure, Tertiary , Proto-Oncogene Proteins c-akt , Signal TransductionABSTRACT
Neuregulins have been implicated in a number of events in cells in the oligodendrocyte lineage, including enhanced survival, mitosis, migration, and differentiation. At least two signaling pathways have been shown to be involved in neuregulin signaling: the phosphatidylinositol (PI)-3 kinase and the mitogen-activated protein kinase pathways. In the present studies, we examined the signaling pathway involved in the survival function of heregulin, focusing on heregulin-induced changes in Akt activity in cultured glial cells, and the consequences of Akt activation in cells in the oligodendrocyte lineage. Heregulin binds erbB receptors, and in our studies, primary cultures of both oligodendrocyte progenitor cells and differentiating oligodendrocytes expressed erbB2, erbB3, and erbB4 receptors. In C6 glioma cells and primary cultures of oligodendrocytes, heregulin induced time- and dose-dependent Akt phosphorylation at Ser(473) in a wortmannin-sensitive manner. To investigate further the signaling pathway for heregulin in glial cells, BAD was overexpressed in C6 glioma cells. In these cells, heregulin induced phosphorylation of BAD at Ser(136). Apoptosis of oligodendrocyte progenitor cells induced by growth factor deprivation was effectively blocked by heregulin in a wortmannin-sensitive manner. Overexpression of dominant negative Akt but not of wild-type Akt by adenoviral gene transfer in primary cultures of both oligodendrocytes and their progenitors induced significant apoptosis through activation of the caspase cascade. The present data suggest that the survival function of heregulin is mediated through the PI-3 kinase/Akt pathway in cells in the oligodendrocyte lineage and that the Akt pathway may be quite important for survival of cells in this lineage.