[Interaction assessment of polysaccharides and minor bioactive compounds in functional food ingredients of plant origin].

At present, the scientific based view of creation enriched, specialized and functional products based on bioactive compounds (BAC) of plant origin has been formed. Interactions between polysaccharides (hydrocolloids), macronutrients of the food system and minor BAC are a determining factor in their bioavailability and should be taken into account when developing formulations and evaluated accordingly. The objective of the research was to consider the theoretical aspects of the interaction of polysaccharides and minor BAC in functional food ingredients of plant origin, as well as to provide an overview of currently available methods for their evaluation. Material and methods. The search and analysis of publications were carried out using the eLIBRARY, PubMed, Scopus, Web of Science databases, mainly in the last 10 years. Results. The main interaction mechanisms of the polysaccharides with minor BAC were determined using the example of the components of the polyphenol complex (flavonoids), ecdysteroids. These include: adsorption, the formation of an "inclusion complex", hydrogen bonding between OH-groups. The interaction of BAC with other macromolecules can occur with their significant modification as a result of the formation of complexes and cause a decrease in biological activity. The assessment of the degree of interaction of hydrocolloids with minor BAC can be carried out using both in vitro and in vivo methods. Most of these studies are carried out in vitro, do not take into account many factors that affect the bioavailability of BAC. Thus, it can be noted that, despite significant progress in the development of functional food ingredients based on medicinal plant materials, the studies of the interactions of BAC with polysaccharides using relevant models are not currently carried out to the extent necessary. Conclusion. Based on the data presented in the review, it can be concluded that plant polysaccharides (hydrocolloids) have a significant effect on the biological activity and availability of minor BAC (polyphenols, ecdysteroids). As an optimal technique for a preliminary assessment of the degree of interaction, it is recommended to use a model that includes the main enzymatic systems, which allows you to accurately reproduce the processes occurring in the gastrointestinal tract; at the final stage, it is necessary to confirm the biological activity in vivo.

[Study of the sorptiondesorption of ecdysterone (20 E) as part of adaptogenic compositions with inulin and functional food ingredients based on spinach and quinoa].

The main principle in the enrichment of food with minor bioactive compounds is the prediction and evaluation of possible chemical interactions of the components included in the matrix of the food. These interactions have a impact on the bioavailability of minor bioactive compounds. In our work, we studied the processes of sorption and desorption (release), the main processes affecting the bioavailability of the minor bioactive compound ecdysterone (20 E) in the composition of functional food ingredients obtained from spinach leaves (FFI-1) and quinoa grains (FFI-2) on hydrocolloid matrix - inulin. The objective of the research was to study the completeness of sorption-desorption processes of 20 E in adaptogenic compositions with inulin and functional food ingredients based on spinach and quinoa under the influence of hydrolytic enzymes of the gastrointestinal tract (GIT) in vitro. Material and methods. To obtain experimental compositions, containing FFI-1 and FFI-2 and the polysaccharide (inulin), a mechanical mixing method was used. To study the sorption properties, model solutions of the compositions were prepared. Using an in vitro enzymatic model, the ability of 20 E to be released from the matrix of the compositions was studied. The content of 20 E was determined by HPLC-MS/MS. Results. 6 compositions with different ratios of polysaccharide/FFI were obtained. At the first stage of the study, the maximum sorption of 20E in the model solution was observed for 4 compositions with the ratio of inulin : FFI = 2.50 or 3.75 g : 189.19 mg FFI-1 or 68.40 mg FFI-2. At the second stage of the study, when assessing the desorption of 20 E on the enzymatic GIT model, it was found that 20 E almost completely released only from 2 compositions, in other cases about 25% of 20 E remained in a bound state. Conclusion. The formulation of two compositions with the ratio of inulin (2.50 g) : FFI-1 (189.19 mg)/FFI-2 (68.40 mg) were obtained, which have the most optimal sorption / release parameters of 20 E under the influence of human gastrointestinal enzymes. These compositions can be considered promising for inclusion in the formulation of fortified foods.

[Development and validation of methods for the quantitative determination of vitamins B1 and B2 in foods by high performance liquid chromatography with diode array detection].

The main sources of vitamins, which are essential substances, are mainly aliment products, foods for special dietary uses and dietary supplements. Therefore, the study of the native content of vitamins in aliment foods has always been of interest. For the chromatographic separation of vitamins, rather versatile C18 columns are used as a stationary phase, which allow one to obtain reliable results using UV detection for vitamin-enriched foods, dietary supplements and vitamin premixes. However, for unfortified foods, this stationary phase in a UV detection system does not give acceptable results. The aim of the work was to develop a technique for the chromatographic separation of vitamins B1 and B2 in unfortified foods using a diode array detector. Material and methods. To prepare samples of foods, concentrated acid hydrolysis (1.0 g of sample and 4 ml of 0.1 N hydrochloric acid) was carried out in a water bath for 30 min at a temperature of 95 °C, followed by enzymatic hydrolysis and degreasing. Further studies of the samples were carried out on an Agilent Technologies 1100 chromatographic system with diode array detection. For the determination of vitamin B1, a Poroshell 120 Hilic column 4.6×150 mm, grain size 2.7 µm was used. As eluent A, a 10 mM aqueous solution of ammonium acetate with 0.5% acetic acid was used, eluent B was acetonitrile (gradient elution: 0-2 min - 90% B, 8-12 min - 50% B, 14-18 min - 90% B). To determine vitamin B2, a C18 Poroshell column 4.6×250 mm, grain size 5 µm was used. As eluent A, a classical phosphate buffer with pH 2.5 was used, eluent B - acetonitrile (gradient elution: 0-5 min - 0% B, 5-15 min - 90% B, 15-22 min - 90% B, 22-24 min - 0% B, 24-27 min - 0% B). Vitamin B1 was detected at a wavelength of 270 nm, vitamin B2 at 450 nm. Under selected conditions, good retention and efficient separation of vitamins B1 (over 16,000 theoretical plates) and B2 (over 20,000 theoretical plates) was observed. Results. It was demonstrated that the HPLC method with diode array detection can be used to quantify the native content of vitamins B1 and B2 in products with a complex food matrix. For the selective determination of these vitamins, a complex of chromatographic conditions is optimal: reverse-phase HPLC for vitamin B2 and hydrophilic interaction chromatography for vitamin B1. A suitable sample preparation of food products for the content of vitamins B1 and B2 under selected chromatographic conditions is concentrated acid-enzymatic hydrolysis. The limit of quantitation for vitamins B1 and B2 was 40 µg/100 g. Comparison of the enzymatic activity of amylorizin and thermostable α-amylase showed that during long-term hydrolysis for 16 hours (37 °Ð¡) with amylorizin, the degree of vitamin extraction was two fold higher than during hydrolysis (95 °Ð¡, 1 h) with α-amylase. Conclusion. The selected conditions for determining the native content of vitamins B1 and B2 in unfortified and low-fortified foods can be used in practice, which has been proven through their successful validation and practical application on real samples of cereals.

[Ways of nutritional value increasing of distillary grain fiber].

In the process of grain processing for ethyl alcohol, practically only carbohydrates are consumed, which are presented mainly in the form of starch. The remaining components (protein, fats, fiber, minerals) in transit pass into the grain fiber remaining after distillation of the alcohol from the mash. Distillery grain fiber surpasses wheat bran in its indicators, since during the processing it is enriched with biomass of alcohol yeast. In addition, there is a technological possibility of its additional enrichment with protein, amino acids, and vitamins due to changes in the modes of alcoholic fermentation. The aim of the work was to assess the influence of the mode and conditions of alcoholic fermentation on the composition of distillery grain fiber. Material and methods. Under laboratory conditions, samples of grain fiber of alcohol production were obtained by the method of fermentation samples from wheat under various fermentation conditions. In the obtained samples, the following parameters were determined: protein according to Barnstein, crude protein, vitamins B1, B2, B6, E, as well as the amino acid composition. The volume fraction of ethyl alcohol, the mass concentration of fermentable carbohydrates, and the concentration of yeast cells were determined in intermediate products of alcohol production. Results and discussion. Studies on the effect of yeast from various manufacturers on fermentation rates, biomass growth and grain fiber composition showed the advantage of race Y-717 in terms of alcohol accumulation (11.5% vol.), Fermentation rate (56 hours) and yeast cell concentration (260 million/cm3), which was 15-30% more than in other options. However, from the point of view of increasing the content of protein according to Barnstein and crude protein in samples of grain fiber with yeast Y-717, an increase of only 3-4% was noted compared with other options. This was associated with a decrease in the concentration of yeast by 72 h of fermentation due to autolysis. Studies on the influence of the initial yeast concentration on the growth of biomass, the fermentation rate and grain fiber indices showed that with an increase in the initial yeast concentration from 15 to 45 million/cm3, the fermentation time reduced to 48 h, the biomass growth at the end of fermentation was 20%, the protein in grain fiber increased by 15%, the content of vitamins B1, B2, B6 and E as well as amino acids increased by 13-17%. Conclusion. According to the results of the studies, the technological possibility of enriching distillery grain fiber with protein, amino acids, and vitamins due to a change in the fermentation process during the processing of grain raw materials to alcohol is shown. In particular, this could be achieved through the use of yeast with a high growth rate, by increasing the concentration of yeast biomass, shortening the fermentation period and preventing yeast autolysis at the maturation stage.

[Databases of the chemical composition of foods in the era of digital nutrition science].

By studying the chemical composition of foods, expanding the list of data on the content of nutrients, including minor biologically active substances, in the era of digital nutrition science, it became possible to create relevant systematic databases of the chemical composition of foods and rations in general. They allow us to solve various problems of modern society from the point of view of nutrition science. This review aim to analyze and generalize modern approaches to the formation and updating of databases of the chemical composition of food products from the standpoint of digital nutrition science. Results. This review considers the main provisions regarding creation of databases, directions for the development of food chemistry, discusses existing international programs for collecting and compiling data. The methods of systematizing data on the qualitative composition and content of biologically active and minor substances in products, as well as the problems associated with the development and metrological certification of highly selective highly sensitive analytical methods necessary to obtain reliable and reproducible data are considered. Conclusion. The development of digital nutrition science significantly increases the availability and quality of information on the chemical composition of foods, and allows it to be updated quickly. Further improvement of the quality of the data presented in the tables of chemical composition is associated with the establishment of stability and relationships between micro- and macro-components, their influence on the safety, stability of the chemical structure, the influence of the physic-chemical characteristics of the matrix on nutritive value of foods, determination of the content of specific minor components, development of relevant regulatory documents.

[Biological value of fruits and berries of Russian production].

Fruits and berries are the most important sources of a wide range of biologically active substances, including vitamin C, carotenoids, flavonoids, anthocyanins. In order to replenish and update data on the content of food and biologically active substances in the tables of the chemical composition of food products, a study of the content of mono- and disaccharides, dietary fiber was carried out; vitamins C, B1, B2 and E, minerals and trace elements; flavonoids (in terms of rutin), anthocyanins; organic and hydroxycinnamic acids, stilbenoids in various varieties of 16 fruit and berry crops. Material and methods. The material for the study was the fruits of promising varieties and selected forms of pome fruit (apple, pear), stone fruit (cherry, plum, apricot), berry (garden strawberry, raspberry, black currant, red currant, gooseberry), non-traditional crops (actinidia, honeysuckle, cornelian cherry, viburnum, sea buckthorn, rosehip) - a total of 208 samples grown at the I.V. Michurin Federal Scientific Center. The B vitamins were determined by the fluorometric method, and vitamin E, organic and hydroxycinnamic acids, carbohydrates, and stilbenoids were determined by HPLC. The amount of anthocyanin pigments was determined by pH differential spectrophotometry, dietary fiber - by enzymatic-gravimetric method, flavonoids - spectrophotometrically. Results and discussion. The main carbohydrate of apricot is sucrose, black currants, cherries, raspberries and honeysuckle contain mainly fructose and glucose. Raspberries and currants are high in fiber. Comparison of the obtained data on the content of vitamins B1, B2, E, flavonoids and anthocyanins in the studied population of varieties in comparison with the published data of tables of the chemical composition of food products in the USA and Russia was carried out. By the content of vitamin C in descending order, the fruits are arranged in a row: black currant > sea buckthorn > honeysuckle > strawberry > red currant > viburnum > gooseberry > raspberry > apple. Berries, making a significant contribution to providing the body with vitamin C, are not an essential source of vitamins B and E. The inclusion of 100 g of fresh fruit in the diet provides about 10% of the recommended dietary intake for potassium (apricot, gooseberry, cherry and black currant), magnesium (apple, cherry, strawberry) and dietary fiber. Honeysuckle and black currant are high in anthocyanins; consumption of 100 g of these berries will ensure adequate intake of these micronutrients. Some varieties of strawberries, apples and pears are rich in hydroxycinnamic acids. The data obtained can be used to refine the indicators in the existing tables of the chemical composition of food products. Conclusion. The obtained data on the composition of fruit and berry products will make it possible to more correctly calculate the nutritional value of rations using questionnaire-survey methods. Combined analysis of the composition of biologically active substances in berries and fruits makes it possible to more reasonably make the choice of a particular product in the dietary correction of the ration of healthy and sick people.

[Nutrient value of fruit in promising strawberry varieties].

One of the current areas in research of fruit and berry crops is the study of their nutritional value and micronutrient composition. Valuable chemical composition of the strawberry determines a wide spectrum of its utilization not only as fresh fruit, but also as a raw material for food industry. Breeding requirements for contemporary strawberry varieties together with yield, large-fruit, important taste and dietary qualities of fruit provide for higher content of biologic active substances. The aim of the research was an integrated assessment of strawberry fruit for content of nutrients and bioactive compounds, as well as mineral elements, and its antioxidant activity. Material and methods. The objects of investigation were berries of 5 strawberry varieties bred in the I.V. Michurin Scientific Centre (Lakomaya, Prazdnichnaya, Privlekatelnaya, Urozhaynaya CGL, Feyerverk) and 9 foreign varieties (Vima Zanta, Dukat, Zefir, Kama, Maryshka, Red Gauntlet, Festivalnaya romashka, Honey, Elsanta) which are promising for cultivation in the Black Soil Region of Russia. The content of soluble solids was determined by the refractometric method, sugars - by Bertrand's method, pectins - by the volumetric method, anthocyanins - by pH-differential spectrophotometry. Total content of antioxidants was defined by amperometric method with recalculation per quercetin. Determination of vitamin B1 was carried out using the fluorometric thiochrome method, vitamin B2 - by fluorometric titration with riboflavin-binding apoprotein after acid-enzymatic hydrolysis, ascorbic acid, vitamin E (tocopherols), niacin, organic and hydroxycinnamic acids - by HPLC, flavonols - spectrophotometrically, minerals and trace elements - by atomic absorption method. Results and discussion. A significant variation in the chemical composition indices depending on the varietal characteristics was found. Strawberry fruit accumulated 8.5-12.0% soluble solids, 5.9-8.7% sugars, 1.5-2.1% of fibre, 0.78-1.12% pectin substances, 0.89-1.45% organic acid; 100 g contain 33.5-48.2 mg vitamin C, 18.3-108.5 mg anthocyanins, 0.024-0.041 mg vitamin B1, 0.012-0.029 mg vitamin B2, 0.10-0.26 mg niacin, 0.26-0.60 mg TE vitamin E, 102-270 mg potassium, 0.15-0.95 mg iron; total antioxidant activity ranged within 180.8-350.0 mg/100 g. High level of anthocyan accumulation (over 80.0 mg/100 g) was observed in Privlekatelnaya, Lakomaya and Feyerverk strawberry varieties. Urozhaynaya CGL, Privlekatelnaya and Lakomaya varieties were characterized by dessert taste. According to the results of the studies Privlekatelnaya variety stood out because of the high complex accumulation of vitamin C, anthocyanins, and high total antioxidant activity. These varieties are recommended for both fresh use and for freezing, as well as for production of functional foods. Conclusion. The obtained data are of interest for further inclusion in the Tables of the chemical composition of Russian food products.

[Development of a modern methodological base for monitoring the content of vitamins in food and food supplements].

The determination of vitamins in various food matrices is necessary for monitoring the quality and safety indicators of food, including the control of the use of vitamins as food additives - food colorings and antioxidants. As well it is necessary to evaluate the level of consumption of vitamins by different age and sex categories of the population. The analysis of the regulatory and methodical basis in the field of determining the content of vitamins in food, including food supplements, has been held. It is shown that the sample preparation process plays an important role in the procedure of determination of vitamins. The modern problems of sample preparation of foods depending on their matrix are considered. The tasks to improve the methodological base, including the harmonization of interstate and national standards of the Russian Federation with international regulatory documents, are marked. It is emphasized that the most promising methods of vitamins' determination for further development are mass-spectrometry and capillary electrophoresis. The selected methods are characterized by high authenticity of the results. Mass-spectrometric detection is characterized by identification reliability. Capillary electrophoresis is characterized of simplicity of analysis.

[Phytoecdysteroids influence on the hormonal status and apoptosis in growing rats].

The impact of the 15-day consumption of Serratula coronata extract containing phytoecdysteroids on some indicators of hormonal status and activity of apoptosis in various organs of growing male Wistar rats (initial body weight 127.8 +/-2.5 sigma) has been studied. The extract from the leaves of Serratula coronata was added to the water of animals of experimental groups 2 and 3 (n = 8 in each group) daily at the dose of 5 and 15 mg phytoecdysteroids per kg of body weight respectively. Animals of the control group 1 (n = 8) received water alone throughout the experiment. Daily volume of drunk fluid was recorded. At the 15th day of the experiment animals were taken out using the decapitation under the light ether anesthesia. The content of corticosterone, prostaglandin E2 and beta-endorphin in rat blood plasma were determined by ELISA test. Plasma level of noradrenaline was determined by HPLC. DNA damage and percentage of apoptotic cells (apoptotic index) were measured in isolated cells of the thymus, heart and brain by single-cell gel electrophoresis (the comet assay). Significantly lower concentration of norepinephrine was detected in plasma of experimental animals from groups 2 and 3 (10.3 +/- 1.1 and 7.2 +/- 0.8 ng/ml, respectively) compared to the same index in the control group (20.4 +/- 3.4 ng/ml). Significant differences of other biochemical parameters for all groups of animals have not been identified. Statistical significant difference in the ratio of corticosterone/norepinephrine compared with control animals was detected for a group of rats consumed the highest dose of phytoecdysteroids. There was no statistically significant difference in DNA fragmentation and apoptosis index in animals consumed phytoecdysteroids in compare with the control group of animals. The absence of the activity of apoptosis in cells of the heart, brain and thymus of rats treated with phytoecdysteroid extract may indicate the safety of its use in the diet of the animals.

[Effect of intragastrically injection of phytoecdysteroids on some indicators of hormonal status in rats].

The experiment in vivo in growing male Wistar rats was carried out. The animals of the experimental 2-4 groups were daily intragastrically injected water solutions of the dried extract from the leaves of Seratulla coronata L. in volume of 1.0 ml, containing 2, 20 and 50 mg of phytoecdysteroids per kg of animal weight, accordingly. Animals of control group were daily injected 1.0 ml of water. The content of phytoecdysteroids in the dry extract was analyzed by high performance liquid chromatography (HPLC). The concentration of the sum of phytoecdysteroids in dry extract was 6.15%, 66% of which was 20-hydroxyecdysone and 23% was 25S-inokosteron. On the 15th day animals were taken out of the experiment by the decapitation. The content of corticosterone, prostaglandin E2 and beta-endorphin in rat blood plasma were determined by ELISA test. The pathological--anatomical analysis and weighing of the liver did not reveal any adverse changes of this organ in the animals of all groups. The average concentration of blood plasma corticosterone reduced with increasing of the dose of the extract injected to the animals, reaching significant differences relative to the control group (60.9 +/- 9.4 ng/ml) for 3 and 4 groups (22.7 +/- 6.6 and 17.6 +/- 7.3 ng/ml, accordingly). Beta-endorphin and prostaglandin E2 levels did not differ. The ratio of the mediator of stress (corticosterone) and inhibitors of stress (beta-endorphin and prostaglandin E2) has been calculated. A monotonic decrease of corticosterone/beta-endorphin and corticosterone/prostaglandin E2 ratio has been found with extract dose increasing. Taken together the results of determination of biochemical parameters of the general adaptation syndrome the dose-dependent stress-protective effect of Seratulla coronata L extract has been demonstrated.

[Determination of chondroitin sulfate in food supplements by capillary zone electrophoresis].

Chondroitin sulfate is widely used as an ingredient in food supplements. A method of capillary zone electrophoresis for qualitative and quantitative analysis of chondroitin sulfate in food supplements has been developed. The system of capillary electrophoresis Agilent 3D CE (USA) with diode array detector (spectral range 190-400 nm, 192 nm was used to quantity), quartz capillary Agilent with effective length 56 cm (USA) (internal diameter 50 microm, temperature 25 degrees C, 30 kV, negative polarity) and 50 mM phosphate buffer (pH 3.5) has been used. Quantity limit of this method was 0.5 g/kg. It was used for determination of content of chondroitin sulfate in 14 food supplements. The chondroitin sulfate was detected in all test samples with deviation from the declared content (25-600 mg per capsule or tablet) at the level of 1 to 9%. The applicability of the elaborated method for assessing of food supplements quality has been shown.

[Nutrition value of tropical and subtropical fruits].

The article is devoted to the study of the chemical composition of tropical and subtropical fruit (avocado, papaya and mango), which are now in great numbers are on the appeared on the Russian market. Due to use technology tropical and subtropical fruits can be implemented in almost all areas and regions of the country. Relatively low cost makes these products quite popular among the people. In domestic scientific literature there are no systematic data describing the chemical composition of these tropical and subtropical fruits sold in the domestic market, while the information needed to calculate food and energy value of diets and culinary products derived from tropical and subtropical fruit. Avocado fruits are sources of insoluble dietary fiber content of which was equal to 12.2%, as well as minerals. The study of the fatty acid composition of lipids avocados showed high content of oleic acid fruit, which accounts for 53.2% of total fatty acids in these fruits. Which makes them a valuable source of unsaturated fatty acids.

[Composition and content of biologically active substances in rose hips].

The paper studies the chemical composition of the powders obtained from the pulp with the skins and seeds of fruits of wild rose hips. Research results have shown that the main fraction of the powder is dietary fiber, powder of seeds of insoluble fiber in 1,6 and 2,3 higher than in the powder of the fruit with a thin skin and pulp, respectively. The greatest amount of carbohydrates and protein found in powders and pulp of the fruit with a thin skin, and lipids predominate in the powder from the seeds. Found that the lipid powder rosehip richest in oleic, linoleic and linolenic acids, the share of oleic acid has 6,4-19,2%, linoleic and linolenic 19,7-45,8 and 23,3-33,9% of the amount of fatty acids. Lipids powders of hips and seeds of rose have higher levels of essential linoleic acid and powder from the pulp with the skins - linolenic acid. In the study established the presence of sterols 7 fractions, the predominant of which is the beta-sitosterol. In the powder from the pulp with the skins found the greatest amount of ascorbic acid, carotenoids, and the powder of seeds - vitamin E. Carotenoids in powders are beta-carotene and lycopene. The high content of ascorbic acid, vitamin E and carotenoids in powder from wild rose hips makes them a good source of antioxidants. Therefore, we studied the possibility of using vegetable powders obtained from hips of wild rose, to enrich biologically active substances such as vitamins C, E and carotenoids, food supply, particularly of health care use. Rosehip powder from the pulp with the skins had the highest antioxidant activity, antioxidant activity of hips powders was 74% of the activity of powder from the pulp with the skins, the lowest antioxidant activity was observed in the powder from the wild rose seeds. That's way, based on the analysis of the chemical composition of rose hip powder found high levels they ascorbic acid, carotenoids, flavonoids,found their high antioxidant activity. It allows to recommend powders produced from the hips, as a source of physiologically functional ingredients for the production of fortified food products, especially medical and prophylactic purposes. The use of such additives will fill the gap in the body of P-active substances, vitamins C and E, beta-carotene, pectin substances.

[Methods quantitative for determination of water-soluble vitamins in premixes and fortified food products by micellar electrokinetic chromatography on short end of the capillary].

It was purposed new technique by micellar electrokinetic chromatography on short end of the capillary (capillary electrophoresis system Agilent 3D CE, DAD, quartz capillary HPCE stndrd cap 56 cm, 50 microm, 50 mM borate buffer pH=9,3, 100 mM sodium dodecil sulfate) for simultaneous determination of water-soluble vitamins (B1, B2, B6, B12, PP, B5, B9, C, B8) in fortified food products and premixes. It was observed on 6 samples of vitamin premixes and 28 samples of fortified food products using this technique. Our findings are consistent with the results of research on certain vitamins, conducted by other methods. The developed technique can be used in analysis of water-soluble vitamins in premixes and fortified food products.

[Development of methods for determining acrylamide in food products by gas-liquid chromatography].

The method of determination of acrylamide in various food (milk powder, potato chips, instant coffee) by gas-liquid chromatography after pre-bromination was developed. Studies have shown the possibility of using bromination of acrylamide to give it the necessary properties for better extraction, purification and detection. Also revealed the possibility of qualitative and quantitative determine a acrylamide in food by gas-liquid chromatography with detection by electron capture detector.

[Determination of melamine in milk and milk-containing products by capillary zone electrophoresis].

For determination of melamine methods of reversed phase and ion-pair high-performance liquid chromatography, and also immune-enzyme analysis are used. The purpose of our work was working out of a technique of determination melamine in milk and lactiferous products method CZE. As a result of researches determination conditions melamine in foodstuff by method CZE (sensitivity from 0.8-1.0 mg/l) are developed, and also conditions of its isolation from objects of research are adapted.

[The new method for simultaneous determination of natural and synthetic food dyes by high performance liquid chromatography].

In the food industry in Russia is currently allowed to use more than 30 different dyes. Existing approaches to monitoring their use in foods are based on spectrophotometry, thin layer chromatography and high performance liquid chromatography (HPLC). Methods of research focused on the analysis of a specific class of food dye--natural or synthetic, and can not be used in the analysis of their mixtures. The aim of work was to develop HPLC method for the joint determination of various classes of dyes in complex food additives and food products. As a result of the research suggested a method to allow a simultaneous determination of at least 15 natural and synthetic food dyes.