A Jakobsian analysis of communication in veterinary anatomic pathology

Since I figured out that anatomic pathology is communication (5), I got interested in this subject and If I'm assuming myself as a communicator, then, I logically need to study and understand it. When I looked for this, Jakobson's explanation (4) was the most elegant, eloquent and simplest explanation I've found. If you doubt me, just search for this in youtube, there are a lot of animated videos about it. So, Jakobson divides communication into six crucial points: adresser, message, addressee, context, code and channel. The adresser is the person who will write and send the message to the addressee, the receptor, the person who will read it. The context is what the message is about and the code is the language used. Finally, the channel is the physical channel, a way where the message is written, it may be a paper or a video or any other. Keep in mind that those points are not always isolated from each other, they are frequently overlapped, and this is just a didactic organization that is much more easily to understand in a fluxogram(AU)

Hematological and biochemical changes in a cow with metastatic cholangiocarcinoma

We describe here hematological and biochemical findings in a cow with metastatic cholangiocarcinoma. On physical examination, apathy with congested mucous membranes, tachycardia and enlarged abdomen; hypoperistalsis in rumen, abomasum and intestines. In hematological examination, icteric plasma with leukocytosis by neutrophilia with regenerative left shift and inversion of the proportion between lymphocytes and segmented neutrophils, and hypofibrinemia. In serum biochemistry: hyperproteinemia, hypoalbuminemia, hyperglobulinemia, low albumin/globulin ratio and high levels of aspartate aminotransferase, gamma-glutamyltransferase. The cow died and in necropsy was observed a severe and diffuse increase in volume predominantly on the hepatic right lobe and moderate increase of volume in mesenteric lymph nodes. Which was diagnosed microscopically as a cholangiocarcinoma, characterized by arrangements in irregular and coalescent ducts. In conclusion, cholangiocarcinoma may cause important hematological and biochemical changes in cattle.(AU)

Histopathology and microscopic morphology of protozoan and metazoan parasites of free ranging armadillos in Brazil

ABSTRACT: This study assessed microscopic morphology of protozoan and metazoan parasites, as well as parasite-associated histopathologic changes in five Brazilian free-ranging armadillos. Three armadillos had intra sarcolemmal cysts of Sarcocystis sp. in skeletal muscles without microscopic changes. One Dasypus novemcinctus was found parasitized with a nematode morphologically compatible with an oxyurid in the small intestine. One Dasypus sp. had neutrophilic enteritis associated with adult and larval stages of Strongyloides sp. and one D. novemcinctus had multiple embryonated eggs free in the lumen of the small intestine with mild neutrophilic enteritis. These findings represent a contribution for expanding our knowledge on parasitic diseases of armadillos.

RESUMO: Este estudo avaliou a morfologia microscópica de parasitos protozoários e metazoários, bem como lesões associadas ao parasitismo em cinco tatus de vida livre no Brasil. Três tatus tinham cistos de Sarcocystis sp. Intra-sarcolemal em músculos esqueléticos sem alterações microscópicas. Um Dasypus novemcinctus estava parasitado com um nematodo morfologicamente compatível com oxiurideo no intestino delgado. Um Dasypus sp. apresentou enterite neutrofílica associada com estágios larvais de Strongyloides sp. e um D. novemcinctus apresentou múltiplos ovos embrionados livres no lúmen do intestino delgado, associado a enterite neutrofílica discreta. Estes achados representam uma contribuição para a expansão do conhecimento sobre doenças parasitárias de tatus.

Histopathology and microscopic morphology of protozoan and metazoan parasites of free ranging armadillos in Brazil / Histopatologia e morfologia microscópica de parasitos protozoários e metazoários de tatus de vida livre no Brasil

This study assessed microscopic morphology of protozoan and metazoan parasites, as well as parasite-associated histopathologic changes in five Brazilian free-ranging armadillos. Three armadillos had intra sarcolemmal cysts of Sarcocystis sp. in skeletal muscles without microscopic changes. One Dasypus novemcinctus was found parasitized with a nematode morphologically compatible with an oxyurid in the small intestine. One Dasypus sp. had neutrophilic enteritis associated with adult and larval stages of Strongyloides sp. and one D. novemcinctus had multiple embryonated eggs free in the lumen of the small intestine with mild neutrophilic enteritis. These findings represent a contribution for expanding our knowledge on parasitic diseases of armadillos.(AU)

Este estudo avaliou a morfologia microscópica de parasitos protozoários e metazoários, bem como lesões associadas ao parasitismo em cinco tatus de vida livre no Brasil. Três tatus tinham cistos de Sarcocystis sp. Intra-sarcolemal em músculos esqueléticos sem alterações microscópicas. Um Dasypus novemcinctus estava parasitado com um nematodo morfologicamente compatível com oxiurideo no intestino delgado. Um Dasypus sp. apresentou enterite neutrofílica associada com estágios larvais de Strongyloides sp. e um D. novemcinctus apresentou múltiplos ovos embrionados livres no lúmen do intestino delgado, associado a enterite neutrofílica discreta. Estes achados representam uma contribuição para a expansão do conhecimento sobre doenças parasitárias de tatus.(AU)

Histopathology and microscopic morphology of protozoan and metazoan parasites of free ranging armadillos in Brazil / Histopatologia e morfologia microscópica de parasitos protozoários e metazoários de tatus de vida livre no Brasil

This study assessed microscopic morphology of protozoan and metazoan parasites, as well as parasite-associated histopathologic changes in five Brazilian free-ranging armadillos. Three armadillos had intra sarcolemmal cysts of Sarcocystis sp. in skeletal muscles without microscopic changes. One Dasypus novemcinctus was found parasitized with a nematode morphologically compatible with an oxyurid in the small intestine. One Dasypus sp. had neutrophilic enteritis associated with adult and larval stages of Strongyloides sp. and one D. novemcinctus had multiple embryonated eggs free in the lumen of the small intestine with mild neutrophilic enteritis. These findings represent a contribution for expanding our knowledge on parasitic diseases of armadillos.(AU)

Este estudo avaliou a morfologia microscópica de parasitos protozoários e metazoários, bem como lesões associadas ao parasitismo em cinco tatus de vida livre no Brasil. Três tatus tinham cistos de Sarcocystis sp. Intra-sarcolemal em músculos esqueléticos sem alterações microscópicas. Um Dasypus novemcinctus estava parasitado com um nematodo morfologicamente compatível com oxiurideo no intestino delgado. Um Dasypus sp. apresentou enterite neutrofílica associada com estágios larvais de Strongyloides sp. e um D. novemcinctus apresentou múltiplos ovos embrionados livres no lúmen do intestino delgado, associado a enterite neutrofílica discreta. Estes achados representam uma contribuição para a expansão do conhecimento sobre doenças parasitárias de tatus.(AU)

Cholelithiasis, choledocholithiasis and hepatolithiasis with secondary necrotizing hepatitis and cholangiohepatitis in a dairy cow

Biliary calculi are rare in cattle and occur usually in the gallbladder, without clinical signs. In humans, cholelithiasis is a common cause of hepatic abscess due calculi microbiota. Here is described a case of cholelithiasis, choledocholithiasis and hepatolithiasis in a 10-year-old female mixed breed dairy cow. The animal died during physical examination with signs as cachexia, icterus, and fever. At necropsy, a large number of green calculi were observed in the gallbladder, common duct lumen and in markedly distended biliary ducts. The liver was firm and decreased in volume with multiple abscess and multiple red foci measuring 0.5 cm in diameter in the hepatic parenchyma. Microscopically in the liver, marked ductal proliferation and abscedative cholangiohepatitis with abundant fibrosis and multiple foci of hepatocytes necrosis. In conclusion, choledocholithiasis and hepatolithiasis may occur in cattle and cause significant clinical signs and pathological alterations.(AU)

Cecal dilatation and distension in a Holstein calf

Cecal dilatation and distention is an important disorder in early lactation dairy cows, however, reports describing the anatomical pathology findings of this condition are scarce in the literature. Etiopathogenesis of cecal dilatation and distention is often attributed to high concentrate feeds, but there is also evidence of myoelectrical dysfunction contributing to its occurrence. Diagnosis is often made based on physical exam findings, with the contribution of ancillary exams. This paper aims to describe a case of cecal dilatation with clinical, laboratorial and pathology findings of a 5-month-old Holstein calf that presented abdominal distension, positive succussion of the right flank and mild dehydration. Clinical pathology findings included neutrophilic leukocytosis with regenerative left shift and elevated ruminal chloride. Ultrasonographic examination of the right abdomen showed distended and hypomotile intestinal loops. Despite that, due to the patient’s age, which prevented rectal palpation, and lack of some characteristic clinical and clinical pathology findings, diagnosis was only possible post mortem. Macroscopical and microscopical findings demonstrated cecum dilatation with edema, hemorrhage and thrombi. Despite being well known by large animal clinics, anatomical pathologists must be aware of this condition.

Cholelithiasis, choledocholithiasis and hepatolithiasis with secondary necrotizing hepatitis and cholangiohepatitis in a dairy cow

Biliary calculi are rare in cattle and occur usually in the gallbladder, without clinical signs. In humans, cholelithiasis is a common cause of hepatic abscess due calculi microbiota. Here is described a case of cholelithiasis, choledocholithiasis and hepatolithiasis in a 10-year-old female mixed breed dairy cow. The animal died during physical examination with signs as cachexia, icterus, and fever. At necropsy, a large number of green calculi were observed in the gallbladder, common duct lumen and in markedly distended biliary ducts. The liver was firm and decreased in volume with multiple abscess and multiple red foci measuring 0.5 cm in diameter in the hepatic parenchyma. Microscopically in the liver, marked ductal proliferation and abscedative cholangiohepatitis with abundant fibrosis and multiple foci of hepatocytes necrosis. In conclusion, choledocholithiasis and hepatolithiasis may occur in cattle and cause significant clinical signs and pathological alterations.

Cecal dilatation and distension in a Holstein calf

Cecal dilatation and distention is an important disorder in early lactation dairy cows, however, reports describing the anatomical pathology findings of this condition are scarce in the literature. Etiopathogenesis of cecal dilatation and distention is often attributed to high concentrate feeds, but there is also evidence of myoelectrical dysfunction contributing to its occurrence. Diagnosis is often made based on physical exam findings, with the contribution of ancillary exams. This paper aims to describe a case of cecal dilatation with clinical, laboratorial and pathology findings of a 5-month-old Holstein calf that presented abdominal distension, positive succussion of the right flank and mild dehydration. Clinical pathology findings included neutrophilic leukocytosis with regenerative left shift and elevated ruminal chloride. Ultrasonographic examination of the right abdomen showed distended and hypomotile intestinal loops. Despite that, due to the patients age, which prevented rectal palpation, and lack of some characteristic clinical and clinical pathology findings, diagnosis was only possible post mortem. Macroscopical and microscopical findings demonstrated cecum dilatation with edema, hemorrhage and thrombi. Despite being well known by large animal clinics, anatomical pathologists must be aware of this condition.(AU)

Mammary carcinoma in a mixed tumor with myeloid metaplasia component presenting Leishmania spp. in a dog

Here is described a 9-years-old, intact female dog with cytopathological analysis from mammary tumors containing Leishmania spp. amastigotes in macrophages. The animal was submitted to euthanasia and necropsy was performed. Histopathological diagnosis was a mammary carcinoma in a mixed tumor, grade I, with myeloid metaplasia component containing macrophages laden with a large amount of amastigotes. For confirmation, immunohistochemistry was used in neoplastic tissue and resulted in strong positivity for Leishmania antibody. Myeloid metaplasia portion of mammary carcinoma in a mixed tumor may be, rarely, a site for diagnosis of Canine Visceral Leishmaniasis.(AU)~ien

Mammary carcinoma in a mixed tumor with myeloid metaplasia component presenting Leishmania spp. in a dog

Here is described a 9-years-old, intact female dog with cytopathological analysis from mammary tumors containing Leishmania spp. amastigotes in macrophages. The animal was submitted to euthanasia and necropsy was performed. Histopathological diagnosis was a mammary carcinoma in a mixed tumor, grade I, with myeloid metaplasia component containing macrophages laden with a large amount of amastigotes. For confirmation, immunohistochemistry was used in neoplastic tissue and resulted in strong positivity for Leishmania antibody. Myeloid metaplasia portion of mammary carcinoma in a mixed tumor may be, rarely, a site for diagnosis of Canine Visceral Leishmaniasis.~ien

Colangitis crónica asociada a la infestación por trematodo hepático causado por Platynosomum fastosum, concurrente con la duplicación de la vesícula biliar en el gato doméstico: presentación de un caso / Chronic cholangitis associated with liver fluke infestation caused by Platynosomum fastosum, concurrent with gallbladder duplication in a domestic cat: case report / Colangite crônica associada à infestação de trematódeo por Platynosomum fastosum, concomitante à vesícula biliar dupla em um gato: relato de caso

Este trabajo pretende informar un caso de un felino de cinco años mestizo, presentando intensa ictericia, petequias y equimosis cutánea generalizada, aumento de enzima ALT, aumento del colesterol sérico y disminución de las proteínas plasmáticas. Se instituyó el tratamiento médico, pero el animal murió dos días después de la intervención clínica. El examen histopatológico mostró colangitis crónica asociada con infestación por trematodos Platynosomum fastosumy verdadera duplicación de la vesícula biliar. Se conclui que los cambios anatómicos asociados con la infestación por parásitos pueden agravar significativamente el cuadro clínico de colangitis crónica y siempre debe ser considerado en el plan de diagnóstico.(AU)

This paper aims to report a case of a 5-years-old, mixed breed cat showing severe jaundice, skin petechiae and ecchymosis, high values of ALT enzyme and serum cholesterol and low plasmatic proteins. A medical treatment was instituted; however the animal died two days after clinical intervention. Anatomicopathological analisys showed a chronic cholangitis associated with liver fluke infestation caused by Platynosomum fastosum, and duplex gallbladder. We conclude that anatomical changes associated with infestation by parasites may significantly aggravate the clinical condition of chronic cholangitis and should always be considered in the diagnostic plan.(AU)

Este trabalho tem o objetivo de relatar um caso de um felino de cinco anos, sem raça definida, apresentando icterícia intensa, petéquias e equimoses cutâneas generalizadas, aumento da enzima ALT, aumento de colesterol sérico e diminuição de proteínas plasmáticas. Foi instituído tratamento médico, entretanto o animal morreu dois dias após a intervenção clínica. No exame anatomopatológico observou-se colangite crônica associada à infestação de trematódeo Platynosomum fastosum e vesícula biliar dupla verdadeira. Conclui-se que alterações anatômicas associadas à infestação por parasitas podem agravar significativamente o quadro clínico de colangite crônica e devem sempre ser consideradas no plano diagnóstico.(AU)

Colangitis crónica asociada a la infestación por trematodo hepático causado por Platynosomum fastosum, concurrente con la duplicación de la vesícula biliar en el gato doméstico: presentación de un caso / Chronic cholangitis associated with liver fluke infestation caused by Platynosomum fastosum, concurrent with gallbladder duplication in a domestic cat: case report / Colangite crônica associada à infestação de trematódeo por Platynosomum fastosum, concomitante à vesícula biliar dupla em um gato: relato de caso

Este trabajo pretende informar un caso de un felino de cinco años mestizo, presentando intensa ictericia, petequias y equimosis cutánea generalizada, aumento de enzima ALT, aumento del colesterol sérico y disminución de las proteínas plasmáticas. Se instituyó el tratamiento médico, pero el animal murió dos días después de la intervención clínica. El examen histopatológico mostró colangitis crónica asociada con infestación por trematodos Platynosomum fastosumy verdadera duplicación de la vesícula biliar. Se conclui que los cambios anatómicos asociados con la infestación por parásitos pueden agravar significativamente el cuadro clínico de colangitis crónica y siempre debe ser considerado en el plan de diagnóstico.

This paper aims to report a case of a 5-years-old, mixed breed cat showing severe jaundice, skin petechiae and ecchymosis, high values of ALT enzyme and serum cholesterol and low plasmatic proteins. A medical treatment was instituted; however the animal died two days after clinical intervention. Anatomicopathological analisys showed a chronic cholangitis associated with liver fluke infestation caused by Platynosomum fastosum, and duplex gallbladder. We conclude that anatomical changes associated with infestation by parasites may significantly aggravate the clinical condition of chronic cholangitis and should always be considered in the diagnostic plan.

Este trabalho tem o objetivo de relatar um caso de um felino de cinco anos, sem raça definida, apresentando icterícia intensa, petéquias e equimoses cutâneas generalizadas, aumento da enzima ALT, aumento de colesterol sérico e diminuição de proteínas plasmáticas. Foi instituído tratamento médico, entretanto o animal morreu dois dias após a intervenção clínica. No exame anatomopatológico observou-se colangite crônica associada à infestação de trematódeo Platynosomum fastosum e vesícula biliar dupla verdadeira. Conclui-se que alterações anatômicas associadas à infestação por parasitas podem agravar significativamente o quadro clínico de colangite crônica e devem sempre ser consideradas no plano diagnóstico.

Granulomatous rhinitis in a case of feline leishmaniasis

This is a case report of leishmaniasis with granulomatous rhinitis in a 2-year-old domestic cat. Clinically, an obstruction in the right nostril was observed, with cytopathological examination positive for Leishmania sp. Euthanasia was performed, and multinodular, diffuse and smooth pinkish lesions were observed in the mucosa of the nasal cavity. Microscopically, nodular lesions corresponded to an intense granulomatous inflammation with macrophages containing large numbers of Leishmania sp. amastigotes. Macrophages containing amastigotes were also occasionally observed in the popliteal lymph node, skin, and spleen. Based on immunohistochemistry, amastigotes were confirmed as Leishmania sp. and Leishmania sp. DNA was also detected by qPCR in lymph nodes samples. Although it is an uncommon manifestation, leishmaniasis should be considered as a differential diagnosis in diseases affecting the nasal cavity in domestic felines.

Detection of bluetongue virus in Brazilian cervids in São Paulo state / Detecção de vírus da língua azul em cervídeos brasileiros no estado de São Paulo

Viral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV - Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the bluetongue virus is involved in hemorrhagic disease outbreaks of deer in Brazil.(AU)

Doenças hemorrágicas virais em cervídeos ocorrem no mundo todo e incluem a doença epizoótica hemorrágica (DEH), língua azul (LA), e doença hemorrágica por adenovírus (DHA). Uma vez que as lesões nas três doenças hemorrágicas são idênticas (enteropatia hemorrágica, edema pulmonar, petéquias sistêmicas e sufusões hemorrágicas), é necessário utilizar técnicas precisas para um diagnóstico etiológico definitivo. Material de arquivo (blocos de parafina) do Departamento de Patologia Veterinária da FCAV - Unesp foi revisado para lesões de doenças hemorrágicas e 42 cervídeos brasileiros de cativeiro e de vida livre foram selecionados e incluídos neste estudo. Tecidos embebidos em parafina foram avaliados usando imunohistoquímica e foram negativos para adenovírus. Usando o RT-PCR em tempo real, o vírus da DEH não foi detectado nos tecidos de nenhum dos casos avaliados. A mesma técnica foi utilizada para detecção do vírus da LA e sete animais positivos (16,66%) foram confirmados após eletroforese em gel de agarose a 4% e sequenciamento genético. As principais alterações macroscópicas observadas nos animais positivos foram conteúdo intestinal hemorrágico, mucosa do trato gastrointestinal avermelhada, úlceras na língua e petéquias em vários órgãos. As alterações microscópicas observadas foram infiltrado inflamatório linfocítico em fígado, rins e pulmões, e hemorragia e congestão em vários órgãos. Todos os casos positivos foram de animais de cativeiro, três fêmeas (dois jovens e um adulto), e quatro jovens do sexo masculino. Este estudo demonstra que o vírus da lingual azul está envolvido nos surtos de doença hemorrágica em veados no Brasil.(AU)

Melanocitoma perivulvar congénito en bovino / Perivulvar bovine congenital melanocytoma / Melanocitoma perivulvar congênito em bovino

El objetivo de este relato es describir lós hallazgos clínico, histológico de um tratamiento quirúrgico de un caso diagnosticado como melanocitoma congênito. Localizado en región perivulvar en una vaca mestiza (cebuina). Neoplasia considerada rara en especie bovina, classificación histológica variable y comportamiento biológico difícil de ser previsto.(AU)

The objective of this report is to describe the clinical, histological and surgical treatment of a case diagnosed as congenital melanocytoma, located in perivulvar region in a half-breed cow (zebu). This neoplasia is considered rare in cattle, of variable histological classification and hard to predict biological behavior.(AU)

O objetivo deste relato é descrever os achados clínicos, histológicos e o tratamento cirúrgico de um caso de melanocitoma congênita. Esta neoplasia é considerada rara na espécie bovina, de classificação histológica variável e comportamento biológico difícil de ser previsto.(AU)

Detection of bluetongue virus in Brazilian cervids in São Paulo state / Detecção de vírus da língua azul em cervídeos brasileiros no estado de São Paulo

Viral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV - Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the bluetongue virus is involved in hemorrhagic disease outbreaks of deer in Brazil.(AU)

Doenças hemorrágicas virais em cervídeos ocorrem no mundo todo e incluem a doença epizoótica hemorrágica (DEH), língua azul (LA), e doença hemorrágica por adenovírus (DHA). Uma vez que as lesões nas três doenças hemorrágicas são idênticas (enteropatia hemorrágica, edema pulmonar, petéquias sistêmicas e sufusões hemorrágicas), é necessário utilizar técnicas precisas para um diagnóstico etiológico definitivo. Material de arquivo (blocos de parafina) do Departamento de Patologia Veterinária da FCAV - Unesp foi revisado para lesões de doenças hemorrágicas e 42 cervídeos brasileiros de cativeiro e de vida livre foram selecionados e incluídos neste estudo. Tecidos embebidos em parafina foram avaliados usando imunohistoquímica e foram negativos para adenovírus. Usando o RT-PCR em tempo real, o vírus da DEH não foi detectado nos tecidos de nenhum dos casos avaliados. A mesma técnica foi utilizada para detecção do vírus da LA e sete animais positivos (16,66%) foram confirmados após eletroforese em gel de agarose a 4% e sequenciamento genético. As principais alterações macroscópicas observadas nos animais positivos foram conteúdo intestinal hemorrágico, mucosa do trato gastrointestinal avermelhada, úlceras na língua e petéquias em vários órgãos. As alterações microscópicas observadas foram infiltrado inflamatório linfocítico em fígado, rins e pulmões, e hemorragia e congestão em vários órgãos. Todos os casos positivos foram de animais de cativeiro, três fêmeas (dois jovens e um adulto), e quatro jovens do sexo masculino. Este estudo demonstra que o vírus da lingual azul está envolvido nos surtos de doença hemorrágica em veados no Brasil.(AU)

Granulomatous rhinitis in a case of feline leishmaniasis

This is a case report of leishmaniasis with granulomatous rhinitis in a 2-year-old domestic cat. Clinically, an obstruction in the right nostril was observed, with cytopathological examination positive for Leishmania sp. Euthanasia was performed, and multinodular, diffuse and smooth pinkish lesions were observed in the mucosa of the nasal cavity. Microscopically, nodular lesions corresponded to an intense granulomatous inflammation with macrophages containing large numbers of Leishmania sp. amastigotes. Macrophages containing amastigotes were also occasionally observed in the popliteal lymph node, skin, and spleen. Based on immunohistochemistry, amastigotes were confirmed as Leishmania sp. and Leishmania sp. DNA was also detected by qPCR in lymph nodes samples. Although it is an uncommon manifestation, leishmaniasis should be considered as a differential diagnosis in diseases affecting the nasal cavity in domestic felines.(AU)

Detection of bluetongue virus in Brazilian cervids in São Paulo state

ABSTRACT: Viral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion hemorrhages), it is necessary to use accurate techniques for a definitive etiologic diagnosis. Archival material (paraffin blocks) at the Department of Veterinary Pathology of FCAV - Unesp was reviewed for lesions of hemorrhagic disease and 42 captive and free-living Brazilian deer were selected to include in this study. Paraffin-embedded tissues were evaluated using immunohistochemistry and tested negative for adenovirus. Using real time RT-PCR, EHD virus was not detected in paraffin-embedded tissues in any of the cases evaluated. The same technique was used for detection of BT virus and seven positive animals (16,66%) were confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in the positive animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Microscopic changes observed were lymphocytic inflammatory infiltrate in liver, kidney and lungs, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. This study demonstrates that the bluetongue virus is involved in hemorrhagic disease outbreaks of deer in Brazil.

RESUMO: Doenças hemorrágicas virais em cervídeos ocorrem no mundo todo e incluem a doença epizoótica hemorrágica (DEH), língua azul (LA), e doença hemorrágica por adenovírus (DHA). Uma vez que as lesões nas três doenças hemorrágicas são idênticas (enteropatia hemorrágica, edema pulmonar, petéquias sistêmicas e sufusões hemorrágicas), é necessário utilizar técnicas precisas para um diagnóstico etiológico definitivo. Material de arquivo (blocos de parafina) do Departamento de Patologia Veterinária da FCAV - Unesp foi revisado para lesões de doenças hemorrágicas e 42 cervídeos brasileiros de cativeiro e de vida livre foram selecionados e incluídos neste estudo. Tecidos embebidos em parafina foram avaliados usando imunohistoquímica e foram negativos para adenovírus. Usando o RT-PCR em tempo real, o vírus da DEH não foi detectado nos tecidos de nenhum dos casos avaliados. A mesma técnica foi utilizada para detecção do vírus da LA e sete animais positivos (16,66%) foram confirmados após eletroforese em gel de agarose a 4% e sequenciamento genético. As principais alterações macroscópicas observadas nos animais positivos foram conteúdo intestinal hemorrágico, mucosa do trato gastrointestinal avermelhada, úlceras na língua e petéquias em vários órgãos. As alterações microscópicas observadas foram infiltrado inflamatório linfocítico em fígado, rins e pulmões, e hemorragia e congestão em vários órgãos. Todos os casos positivos foram de animais de cativeiro, três fêmeas (dois jovens e um adulto), e quatro jovens do sexo masculino. Este estudo demonstra que o vírus da lingual azul está envolvido nos surtos de doença hemorrágica em veados no Brasil.

Melanocitoma perivulvar congénito en bovino / Perivulvar bovine congenital melanocytoma / Melanocitoma perivulvar congênito em bovino

El objetivo de este relato es describir lós hallazgos clínico, histológico de um tratamiento quirúrgico de un caso diagnosticado como melanocitoma congênito. Localizado en región perivulvar en una vaca mestiza (cebuina). Neoplasia considerada rara en especie bovina, classificación histológica variable y comportamiento biológico difícil de ser previsto.

The objective of this report is to describe the clinical, histological and surgical treatment of a case diagnosed as congenital melanocytoma, located in perivulvar region in a half-breed cow (zebu). This neoplasia is considered rare in cattle, of variable histological classification and hard to predict biological behavior.

O objetivo deste relato é descrever os achados clínicos, histológicos e o tratamento cirúrgico de um caso de melanocitoma congênita. Esta neoplasia é considerada rara na espécie bovina, de classificação histológica variável e comportamento biológico difícil de ser previsto.