Necrotizing placentitis in a cow caused by Bacillus cereus / Placentite necrotizante em uma vaca por Bacillus cereus

This report described a case of necrotizing placentitis caused by Bacillus cereus in a cow associated with abortion and maternal lethality. The etiological diagnosis of placentitis by B. cereus was based on histopathology of placenta, cytology and bacterial isolation from intrauterine aminiotic fluid in retained placenta and further characterization of the pathogen by the MALDI-TOF. Although, B. cereus abortions are sporadic, the bacterium has the ability to release necrotizing toxins that can lead to placentitis, fetal death and abortion.(AU)

Este relato descreve a placentite necrotizante causada por Bacillus cereus em uma vaca associada a aborto e mortalidade materna. O diagnóstico etiológico de placentite por B. cereus foi baseado na histopatologia da placenta, citologia e isolamento bacteriano partir do líquido aminiótico em placenta retida e identificação do patógeno pela técnica de MALDI-TOF. Embora abortos por B. cereus sejam esporádicos, a bactéria tem a capacidade de liberar toxinas necrotizantes que podem levar a placentite e aborto.(AU)

Mortalidade fetal e neonatal canina: etiologia e diagnóstico / Canine fetal and neonatal mortality: etiology and diagnosis

Mortalidade fetal e neonatal, devido a causas infecciosas e não-infecciosas, é um problema frequente na criação de cães. Fatores predisponentes envolvem erros no manejo e a imaturidade do feto canino ao final da gestação. Causas não-infecciosas incluem hipóxia, prematuridade, hipotermia, hipoglicemia, doenças genéticas,trauma e intoxicações. As causas infecciosas são bactérias, vírus e parasitas, sendo as doenças bacterianas as mais importantes. A identificação da etiologia da morte fetal e neonatal depende da realização de necropsia,exame histopatológico e pesquisa de agentes infecciosos nos tecidos.(AU)

Fetal and neonatal mortality due to infectious and non-infectious causes is an important problem in dog breeding. Predisposing factors include improper management and the immaturity of canine fetus at the end of pregnancy. Non-infectious causes include hypoxia, prematurity, hypothermia, hypoglycemia, genetic disorders, trauma, and toxicosis. Infectious causes include bacteria, virus, and parasites, with bacterial infections as themost important. A conclusive diagnosis in cases of fetal and neonatal death requires necropsy, histopathology,and detection of infectious agents in tissue samples.(AU)

Mortalidade fetal e neonatal canina: etiologia e diagnóstico / Canine fetal and neonatal mortality: etiology and diagnosis

Mortalidade fetal e neonatal, devido a causas infecciosas e não-infecciosas, é um problema frequente na criação de cães. Fatores predisponentes envolvem erros no manejo e a imaturidade do feto canino ao final da gestação. Causas não-infecciosas incluem hipóxia, prematuridade, hipotermia, hipoglicemia, doenças genéticas,trauma e intoxicações. As causas infecciosas são bactérias, vírus e parasitas, sendo as doenças bacterianas as mais importantes. A identificação da etiologia da morte fetal e neonatal depende da realização de necropsia,exame histopatológico e pesquisa de agentes infecciosos nos tecidos.

Fetal and neonatal mortality due to infectious and non-infectious causes is an important problem in dog breeding. Predisposing factors include improper management and the immaturity of canine fetus at the end of pregnancy. Non-infectious causes include hypoxia, prematurity, hypothermia, hypoglycemia, genetic disorders, trauma, and toxicosis. Infectious causes include bacteria, virus, and parasites, with bacterial infections as themost important. A conclusive diagnosis in cases of fetal and neonatal death requires necropsy, histopathology,and detection of infectious agents in tissue samples.

Serum biochemistry in dogs infected with Ehrlichia canis, Anaplasma platys and Leishmania sp / Bioquímica sérica de cães infectados por Ehrlichia canis, Anaplasma platys e Leishmania sp

Background: Hemoparasitoses have major importance in clinical diseases of small animals for infections are highly prevalent, easily transmitted and difficult to control. Clinical signs are nonspecific, but laboratory abnormalities are frequent, especially changes in serum biochemistry. In veterinary medicine, laboratorial findings may favor the diagnosis of hemoparasitoses and improve the quality of treatment given to these patients. The present study aimed at evaluating changes in serum biochemistry in dogs with molecular diagnosis of hemoparasites (Ehrlichia canis, Anaplasma platys and Leishmania sp.).Materials, Methods & Results: Molecular diagnosis for Ehrlichia canis, Anaplasma platys and Leishmania sp. were obtained in 26 dogs. A complete serum biochemical profile was determined in biochemical analyzer COBAS Mira. Biochemical analyzes, using commercial kits from Synermed, included total and partial protein by colorimetric method, creatinine by the kinetic method, urea, alanine aminotransferase (ALT), aspartate aminotrasnferase (AST), gamma glutamyl transferase (GGT) and alkaline phosphatase by enzymatic method. Considering reference values for canine species, it was observed an increase in the average value for serum urea concentration in dogs with leishmaniasis. These dogs also presented higher globulin concentration, while presenting reduction in serum albumin...(AU)

Serum biochemistry in dogs infected with Ehrlichia canis, Anaplasma platys and Leishmania sp / Bioquímica sérica de cães infectados por Ehrlichia canis, Anaplasma platys e Leishmania sp

Background: Hemoparasitoses have major importance in clinical diseases of small animals for infections are highly prevalent, easily transmitted and difficult to control. Clinical signs are nonspecific, but laboratory abnormalities are frequent, especially changes in serum biochemistry. In veterinary medicine, laboratorial findings may favor the diagnosis of hemoparasitoses and improve the quality of treatment given to these patients. The present study aimed at evaluating changes in serum biochemistry in dogs with molecular diagnosis of hemoparasites (Ehrlichia canis, Anaplasma platys and Leishmania sp.).Materials, Methods & Results: Molecular diagnosis for Ehrlichia canis, Anaplasma platys and Leishmania sp. were obtained in 26 dogs. A complete serum biochemical profile was determined in biochemical analyzer COBAS Mira. Biochemical analyzes, using commercial kits from Synermed, included total and partial protein by colorimetric method, creatinine by the kinetic method, urea, alanine aminotransferase (ALT), aspartate aminotrasnferase (AST), gamma glutamyl transferase (GGT) and alkaline phosphatase by enzymatic method. Considering reference values for canine species, it was observed an increase in the average value for serum urea concentration in dogs with leishmaniasis. These dogs also presented higher globulin concentration, while presenting reduction in serum albumin...

Resposta de células trofoblásticas bovinas à infecção por Brucella abortus: uma abordagem proteômica

Brucella abortus é considerada o principal agente etiológico da brucelose bovina, uma zoonose que provoca grandes perdas econômicas em todo o mundo. O objetivo desse estudo foi determinar o perfil diferencial de expressão proteica de células trofoblásticas bovinas nas fases iniciais da infecção por B. abortus. Foi usado o modelo de explantes confeccionados a partir de membrana cório-alantóidea de fetos no último trimestre de gestação infectados por suspensão de B. abortus 2308 (1.0 x 108 bactérias - MOI 1:1000). No estudo da cinética da infecção foram avaliados os tempos de 0,5, 2, 4 e 8 h pós-infecção no qual foi obtida reprodutibilidade alta entre triplicatas dos géis comparados (Match > 84%; IC > 0,77). O perfil de expressão de proteínas foi similar entre os tempos avaliados (Match > 75%). Os tempos de 0,5 e 4 h pósinfecção apresentaram as maiores diferenças qualitativas entre os géis e foram escolhidos para análise diferencial em gel (DIGE). Nessa análise não foram identificados spots com diferenças quantitativas significativas entre os géis comparados. Por outro lado, 103 spots presentes apenas em um grupo experimental foram selecionados para identificação por espectrometria de massa. As proteínas BLVRA, LGALS7, RPLP1, SCAMP2, TOLLIP, GALM, AHCY, PSAPL1/PSAP, OAT, C1QBP, NDUFS8, PRCII, RAB11A, CALM1, MDH1, PRDX3, ABHD14B, KRT14, KRT7, HMGB1, HEXB, AKR1B1, PDIA3 e TPM4 foram identificadas apenas nos extratos proteicos de células trofoblásticas infectadas. As proteínas identificadas nesse estudo servirão como alvos para novas pesquisas relacionadas à interação patógeno-hospedeiro na infecção por B. abortus

Brucella abortus is considered the main etiological agent of bovine brucellosis, a zoonotic disease that causes great economic losses worldwide. The aim of this study was to determine the differential profile of proteins expression of bovine trophoblast cells in the early stages of infection by B. abortus. Was used the explants model prepared from chorio-alantóidea of fetuses in the last trimester of pregnancy infected by suspending the B. abortus 2308 containing 1.0 x 108 bacteria (MOI 1:1000). In the study of the kinetics of infection was evaluated the times of 0.5, 2, 4 and 8 h post infection with B. abortus. Was obtained high reproducibility between triplicate of gels compared (Match> 84%; CI > 0.77) and the proteins expression profile was similar among the times evaluated (Match > 75%). Times of 0.5 and 4 h post infection showed the highest qualitative differences between the gels and were used for the differential in gel electrophoresis (DIGE). In this analysis have not been identified spots with significant quantitative differences between the compared gels. Moreover, 103 spots are present only in an experimental group were selected for identification by mass spectrometry. The proteins BLVRA, LGALS7, RPLP1, SCAMP2, TOLLIP, GALM, AHCY, PSAPL1/PSAP, OAT, C1QBP, NDUFS8, PRCII, RAB11A, CALM1, MDH1, PRDX3, ABHD14B, KRT14, KRT7, HMGB1, HEXB, AKR1B1, PDIA3 and TPM4 were identified only in protein extracts of trophoblastic cells infected by B. abortus. The proteins identified in this study will serve as targets for further research related to host-pathogen interaction in infection by B. abortus