Effect of adding anethole to the cryopreservation medium (powdered coconut water) on morphology, kinetics, and oxidative stress of buck sperm / Efeito da adição de anethole ao meio de criopreserção (água de coco em pó) na morfologia, cinética e estresse oxidativo do sêmen de bode

The quality of post-thawing goat sperm is critical to the success of artificial insemination protocols and may be influenced by extenders, cryoprotectants, and antioxidant substances. Therefore, the objective of this study was to evaluate the effects of the antioxidant anethole on goat sperm diluted in preservation medium based on powdered coconut water (ACP-101c) and frozen. For that, each ejaculate was submitted to the following treatments: ACP-101c (control); control plus supplementation with 30, 300, or 2000 µg/ mL anethole. The samples were thawed and evaluated for morphology, kinetics, membrane integrity, and reactive oxygen species (ROS). The addition of anethole increased morphological abnormalities (P < 0.05), however, it did not affect sperm kinetics. Flow cytometry analysis showed that sperm cells cryopreserved with 300 µg/mL anethole had lower acrosome integrity than those cryopreserved in other treatments. Evaluation of oxidative stress revealed that cells stored in the presence of 2000 µg/mL anethole had small amounts of ROS when compared to those preserved in the control medium alone or supplemented with 300 µg/mL anethole (P < 0.05). After cryopreservation of sperm with 2000 µg/mL anethole, the highest percentage of viable sperm without ROS was observed (P < 0.05). In conclusion, despite reducing ROS levels, the supplementation of anethole in ACP-101c did not affect sperm kinetics or membrane integrity post-thawing, however, it did cause morphological damage to sperm.(AU)

A qualidade do espermatozoide caprino pós-descongelação é crítica para o sucesso dos protocolos de inseminação artificial e pode ser influenciada por extensores, crioprotetores e substâncias antioxidantes. Portanto, o objetivo deste estudo foi avaliar os efeitos do antioxidante anetole sobre espermatozoides caprinos diluídos em meio de conservação à base de água de coco em pó (ACP-101c) e congelados. Para tanto, cada ejaculado foi submetido aos seguintes tratamentos: ACP-101c (controle); controle mais suplementação com 30, 300 ou 2000 µg / mL de anetole. As amostras foram descongeladas e avaliadas quanto à morfologia, cinética, integridade de membranas e espécies reativas de oxigênio. A adição de anetole aumentou as anormalidades morfológicas (P < 0,05), no entanto, não afetou a cinética dos espermatozoides. A análise da citometria de fluxo mostrou que as células de esperma criopreservadas com 300 µg / mL anethole tinham integridade acrosma menor do que aquelas criopreservadas em outros tratamentos. A avaliação do estresse oxidativo revelou que as células armazenadas na presença de 2000 µg / mL anethole apresentaram pequenas quantidades de ROS quando comparadas às preservadas em meio de controle isoladamente ou suplementadas com 300 µg / mL anethole (P < 0,05). Após a criopreservação de espermatozoides com 2000 µg / mL anethole, observou-se a maior porcentagem de espermatozoides viáveis sem ROS (P < 0,05). A população com espermatozoides viáveis sem ROS foi maior quando utilizado 2.000 µg / mL (P < 0,05). Em conclusão, apesar de reduzir os níveis de ROS, a suplementação de anetole em ACP-101c não afetou a cinética espermática e a integridade da membrana pós-descongelação, entretanto, causou danos morfológicos nos espermatozoides.(AU)

Parâmetros reprodutivos de caprinos da raça Canindé e Alpina Britânica nos Estados do Piauí e Ceará / Reproductive parameters of goats of the breed Canindé and British Alpine in the States of Piauí and Ceará

The objective of this study was to evaluate reproductive parameters of Canindé and British Alpine goatsin the states of Piauí and Ceará by measuring scrotal circumference (CE) and seminal parameters of eightanimals (Canindé (4) and British Alpine (4). Four samples were collected from each animal with the aid of anartificial vagina, after which each ejaculate was evaluated for volume, sperm concentration, total andprogressive mass motility, percentage of motile spermatozoa and vigor. Data were expressed as mean andstandard deviation and analyzed using the statistical program Graphpad Prism®. Although the values ofprogressive motility, scrotal circumference and volume were significantly higher in the British Alpine breed, thetwo races of the present study presented values regarding total motility and vigor within the one recommendedby the CBRA. Thus, the semen of the two breeds can be used for artificial insemination, germplasm bankformation and genetic breeding.(AU)

Protocolos de sincronização do estro e ovulação seguidos de inseminação artificial em tempo fixo em ovelhas deslanadas: Resultados preliminares / Synchronization protocols of oestrus and ovulation followed artificial insemination in fixed time in wooless sheep: Preliminary results

The objective of this study was to evaluate the oestrus and pregnancy using seven and 14 dayssynchronization protocol followed by fixed-time artificial insemination (FTAI) in sheep, 50 ewes were selectedand divided into two groups: G-14 (n = 25) and G-07 (n = 25). Were inserted vaginal sponges that remained forseven and 14 days, time when were applied e.G.C. and Cloroprostenol, this only in the G-07. Oestrus and FTAIwere 55 hours after. It was used sperm diluted in ACP-102 with 5% egg yolk, and refrigerated at 4 °C for fourhours. The results were: oestrus rates of 60% and 84%; pregnancy rates of 56% and 72%; lambing of 48% and68%, for G-07 and G- 14, respectively (P > 0.05). In conclusion, the short protocol was effective insynchronizing estrus wooless sheep, as well as long-term protocol.(AU)

Parâmetros reprodutivos de caprinos da raça Canindé e Alpina Britânica nos Estados do Piauí e Ceará / Reproductive parameters of goats of the breed Canindé and British Alpine in the States of Piauí and Ceará

The objective of this study was to evaluate reproductive parameters of Canindé and British Alpine goatsin the states of Piauí and Ceará by measuring scrotal circumference (CE) and seminal parameters of eightanimals (Canindé (4) and British Alpine (4). Four samples were collected from each animal with the aid of anartificial vagina, after which each ejaculate was evaluated for volume, sperm concentration, total andprogressive mass motility, percentage of motile spermatozoa and vigor. Data were expressed as mean andstandard deviation and analyzed using the statistical program Graphpad Prism®. Although the values ofprogressive motility, scrotal circumference and volume were significantly higher in the British Alpine breed, thetwo races of the present study presented values regarding total motility and vigor within the one recommendedby the CBRA. Thus, the semen of the two breeds can be used for artificial insemination, germplasm bankformation and genetic breeding.

Protocolos de sincronização do estro e ovulação seguidos de inseminação artificial em tempo fixo em ovelhas deslanadas: Resultados preliminares / Synchronization protocols of oestrus and ovulation followed artificial insemination in fixed time in wooless sheep: Preliminary results

The objective of this study was to evaluate the oestrus and pregnancy using seven and 14 dayssynchronization protocol followed by fixed-time artificial insemination (FTAI) in sheep, 50 ewes were selectedand divided into two groups: G-14 (n = 25) and G-07 (n = 25). Were inserted vaginal sponges that remained forseven and 14 days, time when were applied e.G.C. and Cloroprostenol, this only in the G-07. Oestrus and FTAIwere 55 hours after. It was used sperm diluted in ACP-102 with 5% egg yolk, and refrigerated at 4 °C for fourhours. The results were: oestrus rates of 60% and 84%; pregnancy rates of 56% and 72%; lambing of 48% and68%, for G-07 and G- 14, respectively (P > 0.05). In conclusion, the short protocol was effective insynchronizing estrus wooless sheep, as well as long-term protocol.