Parasitos intestinais identificados em pelos de cães em Teresina/Piauí / Intestinal parasites identified in dog hair in Teresina/Piauí

A presença de cães em ambientes domiciliares se tornou bastante comum, porém é preciso manter cuidados com relação aos animais, uma vez que eles podem transmitir patógenos ao homem, causando, assim, doenças zoonóticas; e que uma das formas de transmissão pode ser por ingestão acidental de ovos de parasitos presentes nos pelos. Diante disso, o objetivo deste trabalho foi avaliar a ocorrência de parasitos intestinais em pelos de cães atendidos em duas clínicas veterinárias do município de Teresina/PI. Os pelos colhidos foram levados ao Laboratório de Parasitologia da UFPI para avaliação e passaram por um processo de lavagem por meio de uma técnica modificada para essa finalidade. De 59 amostras de pelos de cães, 11 foram positivas para helmintos ou protozoários, sendo encontrados ovos da família Taeniidae e do gênero Ancylostoma, além de cistos de Giardia spp. e oocistos de Cryptosporidium spp., importantes parasitos de potencial zoonótico. Uma vez parasitados, os cães podem oferecer risco de contaminação para os humanos, tanto por meio das fezes como por meio dos pelos. Conclui-se que ovos de helmintos da família Taeniidae e do gênero Ancylostoma e os protozoários do gênero Giardia e Cryptosporidium podem ficar aderidos nos pelos da região perianal de cães, sendo que as ocorrências destes últimos parasitos são os primeiros relatos nos pelos dessa espécie animal.

The presence of dogs in domestic environments has become quite common, but it is necessary to maintain care of the animals, since they can transmit pathogens to humans causing zoonotic diseases and one of the forms of transmission can be by accidental ingestion of parasite eggs present in the hairs. The objective of this study was to evaluate the occurrence of intestinal parasites in the hair of dogs attended at two veterinary clinics in the city of Teresina, PI. The collected hairs were taken to the Parasitology Laboratory at UFPI for evaluation and underwent a washing process through a technique modified for this purpose. Of 59 dog hair samples, 11 were positive for helminths or protozoa, being found eggs of the family Taeniidae and the genus Ancylostoma, besides to cysts Giardia spp. and oocysts of Cryptosporidium spp., important zoonotic potential parasites. Once parasitized, the dogs can pose a risk of contamination to humans through their faeces or through their hair. It is concluded that the eggs helminths of the Taeniidae family and of the Ancylostoma genus and the protozoa of the Giardia and Cryptosporidium genus can be adhered to the hairs of the perianal region of dogs, and these last parasites are the first reports in the hair in this animals species.

Isolation, expansion, differentiation and growth kinetics essay in mesenchymal stem cells culture from the bone marrow of collared peccaries (Tayassu tajacu)

Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...](AU)

Isolation, expansion, differentiation and growth kinetics essay in mesenchymal stem cells culture from the bone marrow of collared peccaries (Tayassu tajacu)

Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...]

Isolation, culture and differentiation potential of collared peccary (Tayassu tajacu) adipose-derived stem cells

Background: The understanding of cell biology and the isolation of mesenchymal stem cells in wild animals show prospects for conducting pre-clinical trials in these unconventional animals. The collared peccary (Tayassu tajacu) are suiforms that belong to the Artiodáctyla order, Tayassuidae family and Tayassu genus. They adapt easily to captivity conditions that favors their commercial rearing and is an alternative for biodiversity conservation. To evaluate the collared peccary (Tayassu tajacu) as a potential animal model for the isolation of mesenchymal progenitor cells, cell culture and cell differentiation protocols. Materials, Methods & Results: To perform this research we used four collared peccaries (Tayassu tajacu) from the Nucleus of Study and Preservation of Wild Animals (IBAMA/PI No . 02/08-618) from Federal University of Piauí (UFPI). Adipose tissue fragments were collected from the dorsocervical region and dissociated mechanically in laboratory. The material was placed in an incubator containing CO2 - 95% at 37C and the cultures were expanded to fifth passage, evalluating cell concentration and viability. The culture medium alfa-MEM supplemented was changed every three days. The cell kinetics was evaluated in triplicate using growth curve performed during ten days, plating the initial concentration of 5 x 104 cells/mL per well in P3 six-well culture plate...(AU)

Isolation, culture and differentiation potential of collared peccary (Tayassu tajacu) adipose-derived stem cells

Background: The understanding of cell biology and the isolation of mesenchymal stem cells in wild animals show prospects for conducting pre-clinical trials in these unconventional animals. The collared peccary (Tayassu tajacu) are suiforms that belong to the Artiodáctyla order, Tayassuidae family and Tayassu genus. They adapt easily to captivity conditions that favors their commercial rearing and is an alternative for biodiversity conservation. To evaluate the collared peccary (Tayassu tajacu) as a potential animal model for the isolation of mesenchymal progenitor cells, cell culture and cell differentiation protocols. Materials, Methods & Results: To perform this research we used four collared peccaries (Tayassu tajacu) from the Nucleus of Study and Preservation of Wild Animals (IBAMA/PI No . 02/08-618) from Federal University of Piauí (UFPI). Adipose tissue fragments were collected from the dorsocervical region and dissociated mechanically in laboratory. The material was placed in an incubator containing CO2 - 95% at 37C and the cultures were expanded to fifth passage, evalluating cell concentration and viability. The culture medium alfa-MEM supplemented was changed every three days. The cell kinetics was evaluated in triplicate using growth curve performed during ten days, plating the initial concentration of 5 x 104 cells/mL per well in P3 six-well culture plate...