Epidemiological aspects of cattle tick fever in Brazil / Aspectos epidemiológicos da tristeza parasitária bovina no Brasil

The present work reviews the epidemiologic situation of Anaplasma marginale and Babesia spp. infections and the occurrence of cattle tick fever outbreaks in Brazil. In areas of tick fever enzootic instability, environmental conditions interfere with the development of Rhipicephalus (Boophilus) microplus: chilly winter in the southern region, floods in the Pantanal, and low humidity in the Caatinga. In contrast, the climatic conditions of stable zones (Cerrado, Amazon and Atlantic Forest biomes) favor tick development. In enzootic areas, tick fever is uncommon because the animals are in frequent contact with the parasite, acquiring immunity naturally during the period of innate resistance; however, outbreaks may occur when calves become infested by considerable numbers of infected ticks during this period or in adults raised in tick-free environments that become infested for the first time when transporting to stable areas. It is necessary to better understand the disease's risk factors under stable conditions and the implications of the mechanical and other vector transmission of A. marginale. To prevent tick fever outbreaks in Brazil, it is important to develop and use anaplasmosis and babesiosis vaccines in cattle from enzootic unstable regions, especially when animals are moved to stable areas.(AU)

O presente trabalho revisa a situação epidemiológica das infecções por Anaplasma marginale e Babesia spp. e a ocorrência de surtos de Tristeza parasitária bovina (TPB) no Brasil. Em áreas de instabilidade enzoótica, as condições ambientais interferem no desenvolvimento do Rhipicephalus (Boophilus) microplus: o frio do inverno na região Sul; as inundações no Pantanal; e a baixa umidade na Caatinga. Por outra parte, as condições climáticas das zonas de estabilidade (os biomas Cerrado, Amazônia e Mata Atlântica) favorecem o desenvolvimento do carrapato. A TPB não é comum nas áreas estáveis, porque os animais estão em contato frequente com os parasitas, adquirindo imunidade naturalmente. Podem, porém, ocorrer surtos quando um número considerável de carrapatos infectados infesta bezerros, durante o período de resistência inata, ou quando adultos que foram criados em ambientes livres de carrapatos infestam-se, pela primeira vez, ao serem transportados para áreas estáveis. É necessário entender melhor os fatores de risco da doença em condições de estabilidade e a implicação da transmissão de A. marginale de forma mecânica, ou por meio de vetores diferentes do carrapato. Para prevenir surtos de TPB, no Brasil, é necessário desenvolver e usar vacinas em bovinos de regiões de instabilidade, especialmente antes de transportá-los para áreas estáveis.(AU)

Ocorrência de Babesia sp, Ehrlichia canis e Hepatozoon canis em cães domiciliados, em dois municípios do estado do Espírito Santo - Brasil / Occurrence of Babesia sp, Ehrlichia canis and Hepatozoon canis in domiciled dogs in two municipalities of the state of Espírito Santo - Brazil / Ocurrencia de Babesia sp, Ehrlichia canis y Hepatozoon canis em perros domiciliados em dos municipios del estado de Espírito Santo - Brasil

As doenças transmitidas por carrapatos são afecções de grande importância na clínica médica de pequenos animais, devido à alta casuística e ampla distribuição vetorial no território brasileiro. Os principais agentes responsáveis pelas infecções em cães são Babesia sp., Ehrlichia canis e Hepatozoon canis. Os animais infectados são assintomáticos ou apresentam sinais clínicos inespecíficos, sendo necessário a utilização de testes diagnósticos para definição do agente etiológico, e diagnóstico seguro. O objetivo do presente estudo foi determinar a ocorrência desses micro-organismos em cães naturalmente infectados, domiciliados nos municípios de Vila Velha e Anchieta, Espírito Santo, utilizando diferentes testes de detecção: Reação em cadeia polimerase (PCR), sorologia para detecção de anticorpos anti Ehrlichia canis e pesquisa de hematozoários em esfregaço sanguíneo. Foram analisadas 65 amostras de sangue obtidas por venopunção da veia cefálica. No teste de PCR, 4,62% dos animais foram positivos para Babesia vogeli e 1,54% para Ehrlichia canis sendo os resultados para Hepatozoon canis negativos. No teste sorológico para E. canis 90,77% dos animais foram positivos para a presença de anticorpos, e na pesquisa em lâminas de esfregaço sanguíneo 3,02% apresentavam outros hemoparasitas. Os resultados indicam a dispersão desses hemoparasitas na população canina da região de estudo, entretanto com baixa ocorrência. O teste de PCR demonstrou-se como o mais sensível no qual Babesia vogeli foi o agente mais observado.

Tick-borne diseases are of great importance in the medical practice of small animals, due to the high casuistry and wide vectorial distribution in the Brazilian territory. The main agents responsible for infections in dogs are Babesia sp., Ehrlichia canis and Hepatozoon canis. Infected animals are asymptomatic or present nonspecific clinical signs, requiring the use of diagnostic tests to define the etiologic agent, and safe diagnosis. The objective of the present study was to determine the occurrence of these microorganisms in naturally infected dogs domiciled in the municipalities of Vila Velha and Anchieta, Espírito Santo, using different detection tests: polymerase chain reaction (PCR), serology to detect antibodies against Ehrlichia canis and research of hematozoa in blood smears. Sixty-five blood samples obtained by venipuncture of the cephalic vein were analyzed. In the PCR test, 4.62% of the animals were positive for Babesia vogeli and 1.54% for Ehrlichia canis, and the results for Hepatozoon canis were negative. In the serological test for E. canis, 90.77% of the animals were positive for the presence of antibodies, and in there search in blood smear slides, 3.02% presented other hemoparasites. The results indicate the dispersion of these hemoparasites in the canine population of the study region, however with low occurrence. The PCR test proved to be the most sensitive, in which Babesia vogeli was the most observed agent.

Las enfermedades transmitidas por garrapatas son enfermedades de gran importancia en la práctica médica de los pequeños animales, debido a la alta casuística y amplia distribución vectorial en el territorio brasileño. Los principales agentes responsables de las infecciones en los perros son Babesia sp., Ehrlichia canis y Hepatozoon canis. Los animales infectados son asintomáticos o presentan signos clínicos inespecíficos, siendo necesario el uso de pruebas diagnósticas para la definición del agente etiológico, y el diagnóstico seguro. El objetivo del presente estudio fue determinar la ocurrencia de estos microorganismos en perros infectados naturalmente, domiciliados en los municipios de Vila Velha y Anchieta, Espírito Santo, utilizando diferentes pruebas de detección: reacción en cadena de la polimerasa (PCR), serología para detectar anticuerpos anti Ehrlichia canis e investigación de hematozoos en frotis de sangre. Se analizaron sesenta y cinco muestras de sangre obtenidas por venopunción de la vena cefálica. En la prueba PCR, el 4,62% de los animales fueron positivos para Babesia vogeliyel 1,54% paraEhrlichia canis, y los resultados para Hepatozoon canis fueron negativos. En la prueba serológica para E. canis, el 90,77% de los animales fueron positivos a la presencia de anticuerpos, y en la investigación en láminas de frotis de sangre el 3,02% presentaron otros hemoparásitos. Los resultados indican la dispersión de estos hemoparásitos en la población canina de la región de estudio, aunque con una baja presencia. La prueba PCR resultó ser la más sensible, en la que Babesia vogeli fue el agente más observado.

Perfil hematológico e epidemiológico em cães com babesiose, no município de Fortaleza/CE / Hematological and epidemiological profile in dogs with babesiosis in the municipality of Fortaleza/CE

A babesiose canina é uma hemoparasitose, causada por um protozoário do gênero Babesia spp., que parasita os eritrócitos de animais infectados. Este estudo teve como objetivo analisar o perfil hematológico e epidemiológico de cães diagnosticados com babesiose, através da visualização, no esfregaço sanguíneo, de merozoítos no interior dos eritrócitos, na cidade de Fortaleza/CE, durante cinco anos (2015 a 2019). No período, foram observados 38 cães acometidos. O segundo semestre, o período seco, foi o que apresentou um maior número de casos (60,5%). Observou-se maior frequência em cães machos (57,8%), sem raça definida (57,8%), com menos de um ano de idade (73,6%). As alterações hematológicas mais frequentes foram trombocitopenia (86,8%) e anemia (81,5%). Sugere-se, nesses casos, a inclusão da babesiose como diagnóstico diferencial. Apesar da baixa sensibilidade, a pesquisa de hemoparasitos, no esfregaço sanguíneo, pode ser utilizada na rotina clínica.

Canine babesiosis is a hemoparasitosis caused by a protozoan of the genus Babesia spp., that parasitize the erythrocytes of infected animals. This study aimed to analyze the hematological and epidemiological profile of dogs diagnosed with babesiosis, through visualization in blood smear of merozoites inside erythrocytes, in the city of Fortaleza/CE, for five years (2015 to 2019). During the period, 38 affected dogs were observed. The second half, the dry period, was the one with the highest number of cases (60.5%). A higher frequency was observed in male dogs (57.8%), mixed breed (57.8%) under one year of age (73.6%). The most frequent hematological changes were thrombocytopenia (86.8%) and anemia (81.5%). In these cases, it is suggested to include babesiosis as a differential diagnosis. Despite the low sensitivity, the search for hemoparasites in the blood smear can be used in the clinical routine.

Molecular epidemiology of bovine babesiosis in Punjab, Pakistan

Background: Babesiosis is endemic in Pakistan and is one of the most important bovine diseases that causes huge economic losses and high mortality in young animals. This disease is transmitted by a protozoan parasite, which belongs togenus Babesia (Apicomplexa: Piroplasmida: Babesiidae). This disease is very much prevalent in summers followed byrainy season because humid environment is favorable for the growth of these parasites. An epidemiological and molecularstudy was conducted to unveil the prevalence and associated risk factors of Babesia bigemina (B. bigemina) and Babesiabovis (B. bovis) in selected districts i.e., Faisalabad, Toba Tek Singh and Jhang of Punjab, Pakistan.Materials, Methods & Results: A total of 518 (Cattle = 360, Buffalo = 158) blood samples were collected. The sampleswere analyzed by polymerase chain reaction (PCR) and nested PCR (n-PCR) targeting apocytochrome b-genes (CYTb).Chi-square test for univariate analysis was used to analyze the data. The overall prevalence in summer based upon microscopic analysis was 20.55% (37/180) and 13.92% (11/79) in cattle and buffaloes respectively and in winter was 8.80%(16/180), 5.06% (4/79)) in cattle and buffaloes respectively. The samples were further analyzed through conventional PCR(c-PCR) and nested PCR (nPCR). The overall results of conventional PCR in summer showed that 72 cows and buffaloeswere infected with babesiosis. The conventional PCR based results of summer showed that prevalence of babesiosis was29.44% (53/180) in cows and 24.05% (19/79) buffaloes. The results of cPCR during the winter season showed that 12.77%(23/180) and 13.92% (11/79) buffaloes were positive for babesiosis. The overall results of conventional PCR in wintershowed that 34/259 cows and buffaloes were infected with babesiosis. On the other hand, the nested PCR results of summerseason showed that the prevalence of babesiosis in cows was 32.22% (58/180) and...

Molecular epidemiology of bovine babesiosis in Punjab, Pakistan

Background: Babesiosis is endemic in Pakistan and is one of the most important bovine diseases that causes huge economic losses and high mortality in young animals. This disease is transmitted by a protozoan parasite, which belongs togenus Babesia (Apicomplexa: Piroplasmida: Babesiidae). This disease is very much prevalent in summers followed byrainy season because humid environment is favorable for the growth of these parasites. An epidemiological and molecularstudy was conducted to unveil the prevalence and associated risk factors of Babesia bigemina (B. bigemina) and Babesiabovis (B. bovis) in selected districts i.e., Faisalabad, Toba Tek Singh and Jhang of Punjab, Pakistan.Materials, Methods & Results: A total of 518 (Cattle = 360, Buffalo = 158) blood samples were collected. The sampleswere analyzed by polymerase chain reaction (PCR) and nested PCR (n-PCR) targeting apocytochrome b-genes (CYTb).Chi-square test for univariate analysis was used to analyze the data. The overall prevalence in summer based upon microscopic analysis was 20.55% (37/180) and 13.92% (11/79) in cattle and buffaloes respectively and in winter was 8.80%(16/180), 5.06% (4/79)) in cattle and buffaloes respectively. The samples were further analyzed through conventional PCR(c-PCR) and nested PCR (nPCR). The overall results of conventional PCR in summer showed that 72 cows and buffaloeswere infected with babesiosis. The conventional PCR based results of summer showed that prevalence of babesiosis was29.44% (53/180) in cows and 24.05% (19/79) buffaloes. The results of cPCR during the winter season showed that 12.77%(23/180) and 13.92% (11/79) buffaloes were positive for babesiosis. The overall results of conventional PCR in wintershowed that 34/259 cows and buffaloes were infected with babesiosis. On the other hand, the nested PCR results of summerseason showed that the prevalence of babesiosis in cows was 32.22% (58/180) and...(AU)

Babesiose encefálica em cães: aspectos clínicos, epidemiológicos e patológicos / Encephalic babesiosis in dogs: clinical, epidemiological and pathological aspects

Background: Babesiosis is a hemolytic disease caused by protozoa of the genus Babesia spp. responsible for causinganemia, fever and splenomegaly in several animal species. The cerebral involvement of babesiosis in dogs manifests itselfin a hyperacute manner with neurological disorders such as motor incoordination, nystagmus, anisocoria and convulsions.In Brazil there still are no studies which describe the pathological aspects of cerebral babesiosis in dogs together withinformation relating to its clinical aspects and epidemiology. Therefore, this work had as an objective to describe sevencases of canine cerebral babesiosis, emphasizing its epidemiological, clinical and pathological aspects.Materials, Methods & Results: All the records of the necropsies performed in dogs at Animal Pathology Laboratory (LPA/HV) of the Federal University of Campina Grande (UFCG), Patos Campus, Paraíba, Northeast of Brazil were identified. In Theperiod from January 2003 to December 2017. Later, were selected all the cases of cerebral babesiosis. Were included in thestudy the cases in which was possible to detect structures that were morphologically compatible with Babesia spp. trophozoites,intra-erythrocyte in vessels, associated to the presence of lesions in the CNS. For the microscopical description all the histological slides of the cases were revised, in addition to new slides were made with the fragments stored in parafin blocks or withthe material fixed in a 10% formalin solution. The clinical course of the animals studied was hyperacute. In 4 cases, there wassudden death within 24 h, from the appearance of the first symptoms and case 7 presented an evolution of approximately 12 h.The main clinical symptoms described were vocalization, anorexia/hyporexia, jaundice and decubitus. Additionally, the mostconsistente macroscopical findings observed...

Molecular prevalence and factors associated with Babesia vogeli infection in dogs in the Cerrado Mato-Grossense region of Brazil / Prevalência molecular e fatores associados à infecção por Babesia vogeli em cães do cerrado Mato-Grossense

Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p>0.05), greater positivity was observed in dogs<1 year of age, male sex, those with free access to the street, and the presence of ticks. PCR samples positive for Babesia spp. were submitted to sequencing and compared in GenBank and exhibited a high degree of similarity with Babesia vogeli sequences.(AU)

Babesiose canina é uma hemoparasitose comum no Brasil. Causada por parasitos do gênero Babesia, é transmitida por carrapatos ixodídeos e acomete canídeos domésticos e silvestres. O objetivo deste trabalho foi verificar a prevalência molecular da infecção por Babesia spp. em cães residentes em áreas urbanas e rurais do município de Cuiabá, estado de Mato Grosso, Brasil, e relacionar os principais fatores associados à infecção. Para a pesquisa foram avaliados 407 cães usando a PCR. Das 407 amostras analisadas, 10 (2,5%) amplificaram DNA de Babesia spp. Não foi observada associação estatística entre as variáveis pesquisadas (p>0,05), porém observou-se maior positividade em cães com idade inferior a um ano, machos, com livre acesso à rua e com a presença de carrapatos. Amostras positivas nas PCRs para Babesia spp. foram submetidas a sequenciamento e comparadas no GenBank, mostrando alto grau de similaridade com as sequências de B. vogeli.(AU)

Babesiose encefálica em cães: aspectos clínicos, epidemiológicos e patológicos / Encephalic babesiosis in dogs: clinical, epidemiological and pathological aspects

Background: Babesiosis is a hemolytic disease caused by protozoa of the genus Babesia spp. responsible for causinganemia, fever and splenomegaly in several animal species. The cerebral involvement of babesiosis in dogs manifests itselfin a hyperacute manner with neurological disorders such as motor incoordination, nystagmus, anisocoria and convulsions.In Brazil there still are no studies which describe the pathological aspects of cerebral babesiosis in dogs together withinformation relating to its clinical aspects and epidemiology. Therefore, this work had as an objective to describe sevencases of canine cerebral babesiosis, emphasizing its epidemiological, clinical and pathological aspects.Materials, Methods & Results: All the records of the necropsies performed in dogs at Animal Pathology Laboratory (LPA/HV) of the Federal University of Campina Grande (UFCG), Patos Campus, Paraíba, Northeast of Brazil were identified. In Theperiod from January 2003 to December 2017. Later, were selected all the cases of cerebral babesiosis. Were included in thestudy the cases in which was possible to detect structures that were morphologically compatible with Babesia spp. trophozoites,intra-erythrocyte in vessels, associated to the presence of lesions in the CNS. For the microscopical description all the histological slides of the cases were revised, in addition to new slides were made with the fragments stored in parafin blocks or withthe material fixed in a 10% formalin solution. The clinical course of the animals studied was hyperacute. In 4 cases, there wassudden death within 24 h, from the appearance of the first symptoms and case 7 presented an evolution of approximately 12 h.The main clinical symptoms described were vocalization, anorexia/hyporexia, jaundice and decubitus. Additionally, the mostconsistente macroscopical findings observed...(AU)

Molecular survey for the presence of tick-borne haemoparasites in dogs and ticks from the Atlantic Forest environment, Rio de Janeiro state, Brazil / Pesquisa molecular sobre a presença de hemoparasitos transmitidos por carrapatos em cães e carrapatos presentes no ambiente de Mata Atlântica no Estado do Rio de Janeiro, Brasil

Tick-borne diseases have a significant worldwide impact in the veterinary and medical fields. The Serra da Tiririca State Park (PESET) is an Atlantic forest reserve that is home to a wide variety of wild animal species, but is suffering from unauthorised human activities, such as unregulated construction, such as private dwellings and the introduction of domestic pets. The objective of this study is to determine the prevalence of piroplasms and Hepatozoon spp. in dogs and ticks in the Atlantic Forest reserve. Blood samples were taken from 124 asymptomatic canines and 88 Rhipicephalus sanguineus ticks collected from animals inhabiting the PESET. Molecular techniques targeting fragments of the 18s rRNA gene from piroplasms and Hepatozoon spp. were applied to all samples obtained, and one blood sample (0.81%) positive for Babesia vogeli was discovered. All dogs tested negative to Hepatozoon spp. and no tick sample showed positive results for either PCR assay. Despite the low frequency of B. vogeli infection found in dogs that inhabit the PESET, parasitized dogs could be a source of transmission for new vectors, other dogs and even wild canines that are part of the native fauna of the park.(AU)

Doenças transmitidas por carrapatos são capazes de causar impacto significativo na medicina humana e veterinária em todo o mundo. O Parque Estadual Serra da Tiririca (PESET) é uma Reserva ambiental de mata atlântica que abriga uma grande variedade de espécies de animais silvestres, porém, vem sofrendo com a ação de atividades humanas não autorizadas, como as construções irregulares e introdução de animais domésticos. O objetivo deste trabalho foi a detecção de piroplasmas e Hepatozoon spp., em cães e carrapatos. Foram coletadas amostras sanguíneas de 124 caninos assintomáticos e de 88 carrapatos Rhipicephalus sanguineus coletados de animais que habitavam o PESET. Técnicas moleculares tendo como alvo fragmentos do gene 18s rRNA de piroplasmas e de Hepatozoon spp. foram empregadas em todas as amostras obtidas, sendo encontrada uma amostra de sangue (0.81%) positiva para Babesia vogeli. Todos os cães foram negativos à pesquisa de Hepatozoon spp. e nenhuma amostra de carrapato apresentou resultado positivo para ambos os ensaios de PCR. Apesar da baixa frequência de infecção por B. vogeli encontrada nos caninos que habitam o PESET, nós alertamos para a possibilidade de que cães parasitados possam servir de fonte de transmissão para novos vetores, outros cães e até canídeos silvestres, que façam parte da fauna nativa do parque.(AU)

Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil / Detecção molecular, sorológica e parasitológica de Babesia vogeli em cães do estado do Piauí, Brasil

Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region.

Estudos sobre a babesiose canina são escassos no Nordeste do Brasil, apesar das condições climáticas favoráveis ao desenvolvimento do carrapato vetor. Esta pesquisa objetivou determinar a ocorrência de Babesia vogeli em cães amostrados em Teresina, estado do Piauí, região Meio Norte do Brasil, através de métodos diretos e indiretos de diagnóstico, além de realizar análise filogenética das sequências 18S rRNA de piroplasmídeos obtidas no estudo. Foram avaliados 315 cães atendidos em clínicas veterinárias, sob qualquer suspeita clínica. Desses animais, foi colhido sangue por venopunção jugular para Reação de Imunofluorescência Indireta (RIFI) e Reação em Cadeia pela Polimerase (PCR). Além disso, esfregaços de sangue periférico foram realizados para pesquisa direta do parasita. A positividade dos animais foi de 2,2% (7/315) ao esfregaço sanguíneo, 4,8% (15/315) à PCR e 48,6% (153/315) à RIFI. O sequenciamento de amostras positivas à PCR resultou em um fragmento de 602 pb do gene 18S rRNA de piroplasmídeos, cujo alinhamento e análise da sequência revelaram 99% de homologia com isolados de B. vogeli de outras regiões do Brasil, além de outros países. É interessante ressaltar que, comparando isolados em diferentes estados do Nordeste, a homologia pode ser bastante variável. Esses são os primeiros resultados sobre a análise molecular de B. vogeli no Estado do Piauí. Além disso, este estudo demonstra que a babesiose canina é endêmica em cães de Teresina, Nordeste do Brasil, e que a PCR pode ser o método de escolha para diagnóstico da doença nessas áreas.

Molecular, serological, and parasitological detection of Babesia vogeli in dogs in the state of Piauí, Brazil / Detecção molecular, sorológica e parasitológica de Babesia vogeli em cães do estado do Piauí, Brasil

Studies on canine babesiosis in northeastern Brazil are scarce, although the weather conditions in this region are favorable for the development of the tick vector. This study determined the prevalence of Babesia vogeli in dogs sampled in Teresina, state of Piauí, northeast Brazil, using direct and indirect diagnostic methods and performed a phylogenetic analysis of 18S rRNA sequences. A total of 315 dogs were screened during routine care regardless of clinical suspicion. Blood was collected by jugular venipuncture to perform indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR) and for parasite screening in peripheral blood smears. Positivity was 2.2% (7/315) by microscopy, 4.8% (15/315) by PCR, and 48.6% (153/315) by IFA. PCR amplified a 602-bp fragment of the piroplasmid 18S rRNA gene, and sequence alignment and analysis revealed 99% homology with B. vogeli isolates from other regions of Brazil and other countries. In addition, there was high variability among sequences from other northeast states of Brazil. This study is the first to perform the molecular analysis of B. vogeli in Piauí. The results demonstrate that canine babesiosis is endemic in dogs sampled in Teresina and that PCR may be the method of choice to perform parasite screening in this region.(AU)

Estudos sobre a babesiose canina são escassos no Nordeste do Brasil, apesar das condições climáticas favoráveis ao desenvolvimento do carrapato vetor. Esta pesquisa objetivou determinar a ocorrência de Babesia vogeli em cães amostrados em Teresina, estado do Piauí, região Meio Norte do Brasil, através de métodos diretos e indiretos de diagnóstico, além de realizar análise filogenética das sequências 18S rRNA de piroplasmídeos obtidas no estudo. Foram avaliados 315 cães atendidos em clínicas veterinárias, sob qualquer suspeita clínica. Desses animais, foi colhido sangue por venopunção jugular para Reação de Imunofluorescência Indireta (RIFI) e Reação em Cadeia pela Polimerase (PCR). Além disso, esfregaços de sangue periférico foram realizados para pesquisa direta do parasita. A positividade dos animais foi de 2,2% (7/315) ao esfregaço sanguíneo, 4,8% (15/315) à PCR e 48,6% (153/315) à RIFI. O sequenciamento de amostras positivas à PCR resultou em um fragmento de 602 pb do gene 18S rRNA de piroplasmídeos, cujo alinhamento e análise da sequência revelaram 99% de homologia com isolados de B. vogeli de outras regiões do Brasil, além de outros países. É interessante ressaltar que, comparando isolados em diferentes estados do Nordeste, a homologia pode ser bastante variável. Esses são os primeiros resultados sobre a análise molecular de B. vogeli no Estado do Piauí. Além disso, este estudo demonstra que a babesiose canina é endêmica em cães de Teresina, Nordeste do Brasil, e que a PCR pode ser o método de escolha para diagnóstico da doença nessas áreas.(AU)

Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil / Levantamento molecular e diversidade genética de piroplasmídeos em equídeos do Centro-Oeste do Brasil

We evaluated the distribution of piroplasmids in equids from the Mato Grosso state in Midwestern Brazil using molecular methods and the interspecific genetic diversity. For this, 1,624 blood samples of equids from 973 farms were examined by PCR, using primer pairs that amplify a fragment of the genes rap-1 and ema-1 of Babesia caballi and Theileria equi, respectively. For molecular characterization and phylogenetic studies, 13 and 60 sequences of the rap-1 and ema-1 genes, respectively, were used to build a dendogram using maximum parsimony. B. caballi and T. equi were detected in 4.11% and 28.16% of the farms, respectively, and molecular prevalence was 2.74% for B. caballi and 25.91% for T. equi. The location of the farms and animals raised in the Pantanal ecoregion influence the probability of equids testing positive for B. caballi and T. equi . Moreover, age and herd purpose were variables significantly associated with T . equi infection. The sequences of B. caballi presented 1.95% intraspecific variability, contrasting with 2.99% in T. equi. Dendrograms for both species demonstrated the presence of subgroups with high values of support of branches. However, it is not possible to associate these groups with geographic origin and/or ecoregion.(AU)

Foi avaliada a distribuição de piroplasmídeos em equídeos do Estado de Mato Grosso, no Centro-Oeste do Brasil, utilizando-se métodos moleculares e a diversidade genética interespecífica. Para isso, 1.624 amostras de sangue de equídeos de 973 fazendas foram examinadas pela PCR, usando pares de oligonucleotídeos que amplificam um fragmento dos genes rap-1and ema-1 de Babesia caballi e Theileria equi, respectivamente. Para caracterização molecular e estudos filogenéticos, foram utilizadas 13 e 60 sequências dos genes rap-1 e ema-1, respectivamente, para construção de um dendograma utilizando máxima parcimônia. B. caballi e T. equi foram detectados em 4,11% e 28,16% das fazendas, respectivamente, e a prevalência molecular foi de 2,74% para B. caballi e 25,91% para T. equi. A localização das fazendas e animais criados na ecorregião do Pantanal influenciam a probabilidade de equídeos serem positivos para B. caballi e T. equi. Além disso, idade e propósito do rebanho foram variáveis, significativamente, associadas à infecção por T. equi. As sequências de B . caballi apresentaram variabilidade intraespecífica de 1,95%, contrastando com 2,99% em T. equi. Dendrogramas para ambas as espécies demonstraram a presença de subgrupos com altos valores de sustentação dos ramos. No entanto, não é possível associar esses grupos com origem geográfica e/ou ecorregião.(AU)

Dynamics of natural infection by Babesia bovis and Babesia bigemina in dairy cattle from an enzootic instability area in Northeastern Brazil

This study aimed to determine the dynamics of natural infection in the transmission of Babesia spp. to cattle in an enzootic instability area in Northeastern Brazil. Blood samples were collected from 30 calves located on two dairy farms to determine the packed cell volume (PCV) and the timing of the primo-infection using polymerase chain reaction (PCR) and their association with climatic factors and management practices. On Farm A, the determination of primo-infection was observed on average at 249.4 (±24.42) days of age for B. bigemina and at 252.6 (±17.07) days of age for B. bovis; there was no significant difference between the times of infection (P> 0.05). The infection coincided with a period of high rainfall in the region. On Farm B, primo-infection infection was not observed. There was no infection by Babesia spp. on Farm B due to the intensive use of acaricides that led to an absence of ticks. There was no significant difference between the average PCV of animals from Farms A and B (P> 0.05). The management practices on the properties, in addition to the weather conditions influenced the exposure of the animals to disease vectors and may have contributed to the maintenance of this enzootic area in Northeastern Brazil.(AU)

Este estudo teve como objetivo determinar a dinâmica da infecção natural na transmissão de Babesia spp. em bovinos de uma área de instabilidade enzoótica no Nordeste do Brasil. Foram coletadas amostras de sangue de 30 bezerras, proveniente de duas propriedades leiteiras para determinação do volume globular e da primo-infecção por meio da reação em cadeia da polimerase associando aos fatores climáticos e medidas de manejo. Na fazenda A, o período médio da primo-infecção para B. bigemina, determinado por meio da PCR, foi de 249,4 (±24,42) dias de idade, enquanto que para B. bovis foi aos 252,6 (±17,07) dias de idade, não existindo diferença estatística. A infecção coincidiu com o período de alta precipitação pluviométrica na região. Não houve infecção por Babesia spp. na fazenda B, na qual o uso intensivo de acaricidas determinou ausência de carrapatos. Não houve diferença significativa entre médias de VG dos animais das fazendas A e B. O manejo adotado nas fazendas estudadas, associado às condições climáticas, interferem na exposição dos animais aos vetores, podendo favorecer a manutenção de uma área de instabilidade enzoótica no Nordeste do Brasil.(AU)

Tristeza Parasitária em bovinos do semiárido pernambucano / Cattle Tick Fever in semi-arid of Pernambuco

This study aimed to determine the seroprevalence of Babesiosis and Anaplasmosis in cattle from the municipalities of Ouricuri and Petrolina, state of Pernambuco, Brazil, and to define the risk factors for the occurrence of the diseases. Blood samples were collected for serologic testing by Indirect Immunofluorescence Assay (IFA). Sanitary epidemiological questionnaires were applied to the producers aiming to identify possible risk factors. Ticks were collected, identified and tested by Polymerase Chain Reaction (PCR) for the diagnosis of infection by Anaplasma marginale, Babesia bigemina and Babesia bovis. The study was conducted with 861 cattle, being 468 in Petrolina and 393 in Ouricuri. The seroprevalence of A. marginale, B. bigemina and B. bovis in Petrolina was of 35.0% (164/468), 35.9% (168/468) and 32.3% (151/468), respectively; and in Ouricuri was 45.5% (179/393), 38.6% (152/393), and 54.9% (216/393), respectively. Co-infection for Anaplasma spp. and Babesia spp. was observed in 31.6% and 32.1% samples of Petrolina and Ouricuri, respectively. The detection of DNA of Babesia spp. by PCR was possible in 5.8% (8/137) ticks; which 62.5% (5/8) was detected later infection with B. bovis; and 23.3% (32/137) with A. marginale. The presence of ticks, the use of acaricide, age, race, and county of residence of the animals were identified as risk factors for TBD by univariate analysis and multivariate. This study allowed the characterization of the municipalities studied as enzootic instability areas for these hemoparasitic, and consequently alert for adoption of adequate control measures and new studies.

Este estudo objetivou determinar a soroprevalência da Babesiose e Anaplasmose em bovinos dos municípios de Ouricuri e Petrolina, estado de Pernambuco, Brasil; e definir os possíveis fatores de risco para a ocorrência dessas doenças. Amostras de sangue foram coletadas para realização de teste sorológico por Imunofluorescência Indireta (RIFI). Questionários epidemiológicos sanitários foram aplicados aos produtores com o objetivo de identificar possíveis fatores de risco. Carrapatos foram coletados, identificados e testados por Reação em Cadeia da Polimerase (PCR) para o diagnóstico da infecção por Anaplasma marginale, Babesia bigemina e Babaesia bovis. O estudo foi conduzido com 861 bovinos, sendo 468 de Petrolina e 393 de Ouricuri. A soroprevalência de A. marginale, B. bigemina e B. bovis em Petrolina foi de 35,0% (164/468), 35,9% (168/468) e 32,3% (151/468), respectivamente; e em Ouricuri foi de 45,5% (179/393), 38,6% (152/393) e 54,9% (216/393), respectivamente. A co-infecção por Anaplasma spp. e Babesia spp. foi observada em 31,6% e 32,1% de amostras de Petrolina e Ouricuri, respectivamente. A detecção de DNA de Babesia spp. por PCR foi possível em 5,8% (8/137) carrapatos, dos quais em 62,5 % (5/8) foi detectada posteriormente infecção por B. bovis, e em 23,3% (32/137) por A. marginale. A presença de carrapatos, o uso de acaricidas, idade, raça, e o município de residência dos animais foram identificados como fatores de risco para TPB pela análise univariável e multivariável. Este estudo permitiu caracterizar os municípios estudados como de instabilidade enzoótica para esses hemoparasitos, e consequentemente, alertar para adoção de medidas adequadas de controle e realização de novos estudos.

Tristeza Parasitária em bovinos do semiárido pernambucano / Cattle Tick Fever in semi-arid of Pernambuco

Este estudo objetivou determinar a soroprevalência da Babesiose e Anaplasmose em bovinos dos municípios de Ouricuri e Petrolina, estado de Pernambuco, Brasil; e definir os possíveis fatores de risco para a ocorrência dessas doenças. Amostras de sangue foram coletadas para realização de teste sorológico por Imunofluorescência Indireta (RIFI). Questionários epidemiológicos sanitários foram aplicados aos produtores com o objetivo de identificar possíveis fatores de risco. Carrapatos foram coletados, identificados e testados por Reação em Cadeia da Polimerase (PCR) para o diagnóstico da infecção por Anaplasma marginale, Babesia bigemina e Babaesia bovis. O estudo foi conduzido com 861 bovinos, sendo 468 de Petrolina e 393 de Ouricuri. A soroprevalência de A. marginale, B. bigemina e B. bovis em Petrolina foi de 35,0% (164/468), 35,9% (168/468) e 32,3% (151/468), respectivamente; e em Ouricuri foi de 45,5% (179/393), 38,6% (152/393) e 54,9% (216/393), respectivamente. A co-infecção por Anaplasma spp. e Babesia spp. foi observada em 31,6% e 32,1% de amostras de Petrolina e Ouricuri, respectivamente. A detecção de DNA de Babesia spp. por PCR foi possível em 5,8% (8/137) carrapatos, dos quais em 62,5 % (5/8) foi detectada posteriormente infecção por B. bovis, e em 23,3% (32/137) por A. marginale. A presença de carrapatos, o uso de acaricidas, idade, raça, e o município de residência dos animais foram identificados como fatores de risco para TPB pela análise univariável e multivariável. Este estudo permitiu caracterizar os municípios estudados como de instabilidade enzoótica para esses hemoparasitos, e consequentemente, alertar para adoção de medidas adequadas de controle e realização de novos estudos.(AU)

This study aimed to determine the seroprevalence of Babesiosis and Anaplasmosis in cattle from the municipalities of Ouricuri and Petrolina, state of Pernambuco, Brazil, and to define the risk factors for the occurrence of the diseases. Blood samples were collected for serologic testing by Indirect Immunofluorescence Assay (IFA). Sanitary epidemiological questionnaires were applied to the producers aiming to identify possible risk factors. Ticks were collected, identified and tested by Polymerase Chain Reaction (PCR) for the diagnosis of infection by Anaplasma marginale, Babesia bigemina and Babesia bovis. The study was conducted with 861 cattle, being 468 in Petrolina and 393 in Ouricuri. The seroprevalence of A. marginale, B. bigemina and B. bovis in Petrolina was of 35.0% (164/468), 35.9% (168/468) and 32.3% (151/468), respectively; and in Ouricuri was 45.5% (179/393), 38.6% (152/393), and 54.9% (216/393), respectively. Co-infection for Anaplasma spp. and Babesia spp. was observed in 31.6% and 32.1% samples of Petrolina and Ouricuri, respectively. The detection of DNA of Babesia spp. by PCR was possible in 5.8% (8/137) ticks; which 62.5% (5/8) was detected later infection with B. bovis; and 23.3% (32/137) with A. marginale. The presence of ticks, the use of acaricide, age, race, and county of residence of the animals were identified as risk factors for TBD by univariate analysis and multivariate. This study allowed the characterization of the municipalities studied as enzootic instability areas for these hemoparasitic, and consequently alert for adoption of adequate control measures and new studies.(AU)

Babesia spp. and Ehrlichia chaffeensis infection in dogs from southeastern Bahia, Brazil

Background: Tickborne diseases are frequent in tropical countries such as Brazil. Protozoa of the Babesia genus and bacteria of the Ehrlichia genus spread throughout the country with high prevalences in urban and rural areas, causing clinical or subclinical diseases in dogs. This study aimed to investigate the prevalence of infection from Babesia spp. and Ehrlichia chaffeensis in the dog population in the municipality of Ituberá, Bahia, Brazil, and to verify the risk factors associated with the infections. Materials, Methods & Results: A cross-sectional study was conducted, consisting of the following procedures: clinical examination and blood samples collection from 380 dogs and application of a structure questionnaire to dog owners to collect epidemiological data. All dogs were evaluated for the presence of ticks and clinical signs associated with the infections. Blood samples were collected and tested for Babesia spp. through capillary blood smears, indirect immunofluorescence assays (IFAT), and polymerase chain reaction (PCR); all the samples were also tested for E. chaffeensis through nested PCR. Intra-erythrocyte piroplasms were visualized in the blood smears of two animals (2/380; 0.5%) in the cytology exams. Anti-B. canis antibodies were detected in 140/380 (36.8%) dogs, at 1:40 dilution. By PCR, 147/380 (38.7%) dogs tested positive for infection by Babesia sp., but no animal was infected by E. chaffeensis. Only 115/380 dogs (30.3%) were infested by ticks. In total, 223/380 dogs (58.7%) were found infected by Babesia spp. No clinical signs were it found to be significant for the infection. The infected (Ht = 40%) and uninfected dogs’ (Ht = 39%) hematocrit averages were not found to significantly differ (P = 0.47). No hematological changes were found to be significant for the disease. The evaluated variables sex, habitat (urban or rural) […]

Babesia spp. and Ehrlichia chaffeensis infection in dogs from southeastern Bahia, Brazil

Background: Tickborne diseases are frequent in tropical countries such as Brazil. Protozoa of the Babesia genus and bacteria of the Ehrlichia genus spread throughout the country with high prevalences in urban and rural areas, causing clinical or subclinical diseases in dogs. This study aimed to investigate the prevalence of infection from Babesia spp. and Ehrlichia chaffeensis in the dog population in the municipality of Ituberá, Bahia, Brazil, and to verify the risk factors associated with the infections. Materials, Methods & Results: A cross-sectional study was conducted, consisting of the following procedures: clinical examination and blood samples collection from 380 dogs and application of a structure questionnaire to dog owners to collect epidemiological data. All dogs were evaluated for the presence of ticks and clinical signs associated with the infections. Blood samples were collected and tested for Babesia spp. through capillary blood smears, indirect immunofluorescence assays (IFAT), and polymerase chain reaction (PCR); all the samples were also tested for E. chaffeensis through nested PCR. Intra-erythrocyte piroplasms were visualized in the blood smears of two animals (2/380; 0.5%) in the cytology exams. Anti-B. canis antibodies were detected in 140/380 (36.8%) dogs, at 1:40 dilution. By PCR, 147/380 (38.7%) dogs tested positive for infection by Babesia sp., but no animal was infected by E. chaffeensis. Only 115/380 dogs (30.3%) were infested by ticks. In total, 223/380 dogs (58.7%) were found infected by Babesia spp. No clinical signs were it found to be significant for the infection. The infected (Ht = 40%) and uninfected dogs (Ht = 39%) hematocrit averages were not found to significantly differ (P = 0.47). No hematological changes were found to be significant for the disease. The evaluated variables sex, habitat (urban or rural) […](AU)

Tristeza Parasitária em bovinos do semiárido pernambucano / Cattle Tick Fever in semi-arid of Pernambuco

This study aimed to determine the seroprevalence of Babesiosis and Anaplasmosis in cattle from the municipalities of Ouricuri and Petrolina, state of Pernambuco, Brazil, and to define the risk factors for the occurrence of the diseases. Blood samples were collected for serologic testing by Indirect Immunofluorescence Assay (IFA). Sanitary epidemiological questionnaires were applied to the producers aiming to identify possible risk factors. Ticks were collected, identified and tested by Polymerase Chain Reaction (PCR) for the diagnosis of infection by Anaplasma marginale, Babesia bigemina and Babesia bovis. The study was conducted with 861 cattle, being 468 in Petrolina and 393 in Ouricuri. The seroprevalence of A. marginale, B. bigemina and B. bovis in Petrolina was of 35.0% (164/468), 35.9% (168/468) and 32.3% (151/468), respectively; and in Ouricuri was 45.5% (179/393), 38.6% (152/393), and 54.9% (216/393), respectively. Co-infection for Anaplasma spp. and Babesia spp. was observed in 31.6% and 32.1% samples of Petrolina and Ouricuri, respectively. The detection of DNA of Babesia spp. by PCR was possible in 5.8% (8/137) ticks; which 62.5% (5/8) was detected later infection with B. bovis; and 23.3% (32/137) with A. marginale. The presence of ticks, the use of acaricide, age, race, and county of residence of the animals were identified as risk factors for TBD by univariate analysis and multivariate. This study allowed the characterization of the municipalities studied as enzootic instability areas for these hemoparasitic, and consequently alert for adoption of adequate control measures and new studies.(AU)

Este estudo objetivou determinar a soroprevalência da Babesiose e Anaplasmose em bovinos dos municípios de Ouricuri e Petrolina, estado de Pernambuco, Brasil; e definir os possíveis fatores de risco para a ocorrência dessas doenças. Amostras de sangue foram coletadas para realização de teste sorológico por Imunofluorescência Indireta (RIFI). Questionários epidemiológicos sanitários foram aplicados aos produtores com o objetivo de identificar possíveis fatores de risco. Carrapatos foram coletados, identificados e testados por Reação em Cadeia da Polimerase (PCR) para o diagnóstico da infecção por Anaplasma marginale, Babesia bigemina e Babaesia bovis. O estudo foi conduzido com 861 bovinos, sendo 468 de Petrolina e 393 de Ouricuri. A soroprevalência de A. marginale, B. bigemina e B. bovis em Petrolina foi de 35,0% (164/468), 35,9% (168/468) e 32,3% (151/468), respectivamente; e em Ouricuri foi de 45,5% (179/393), 38,6% (152/393) e 54,9% (216/393), respectivamente. A co-infecção por Anaplasma spp. e Babesia spp. foi observada em 31,6% e 32,1% de amostras de Petrolina e Ouricuri, respectivamente. A detecção de DNA de Babesia spp. por PCR foi possível em 5,8% (8/137) carrapatos, dos quais em 62,5 % (5/8) foi detectada posteriormente infecção por B. bovis, e em 23,3% (32/137) por A. marginale. A presença de carrapatos, o uso de acaricidas, idade, raça, e o município de residência dos animais foram identificados como fatores de risco para TPB pela análise univariável e multivariável. Este estudo permitiu caracterizar os municípios estudados como de instabilidade enzoótica para esses hemoparasitos, e consequentemente, alertar para adoção de medidas adequadas de controle e realização de novos estudos.(AU)

Hematological parameters and seroprevalence of Ehrlichia canis and Babesia vogeli in dogs / Parâmetros hematológicos e soroprevalência de Ehrlichia canis e Babesia vogeli em cães

The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI95 17.7% 30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI95 40.0% 59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P 0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis.

O objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e anti-Babesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% - 30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% - 59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil / Epidemiologia molecular de Anaplasma platys, Ehrlichia canis e Babesia vogeli em cães não domiciliados do Paraná

Hemoparasitic infections are tick-borne diseases, which affect animals and humans. Considering the importance of canine hemoparasitic infections in veterinary clinics, this study aimed to determine the occurrence of Anaplasma platys, Ehrlichia canis and Babesia vogeli in blood samples from 182 dogs not domiciled in the city of Pato Branco, southwestern region of Paraná State, Brazil, using polymerase chain reaction (PCR). The prevalence of A. platys and B. vogeli was 32.9% and 10.9% respectively, and A. platys infection prevailed (p<0.001). The number of dogs positive for A. platys was larger in Winter (p<0.05). All blood samples were negative for E. canis. In the dogs, infestation by Amblyomma cajennense predominated over that by Rhipicephalus sanguineus (p<0.001); but there was no significant association between PCR and the variables presence of ticks, sex and age. Dogs infected by A. platys and B. vogeli showed thrombocytopenia, lymphopenia and leukocytosis; but there was no correlation between such hematological changes and infection by hemoparasites. This appears to be the first molecular study that demonstrates the existence of A. platys and B. vogeli in dogs from the southwestern region of Paraná.(AU)

As hemoparasitoses são enfermidades transmitidas por carrapatos que afetam os animais e os humanos. Considerando a importância das hemoparasitoses caninas na clínica médica veterinária, este estudo objetivou determinar a ocorrência de Anaplasma platys, Ehrlichia canis e Babesia vogeli em amostras de sangue de 182 cães não domiciliados do município de Pato Branco, região sudoeste paranaense, Brasil, utilizando a reação em cadeia da polimerase (PCR). A prevalência de A. platys e B. vogeli foi de 32,9% e 10.9%, respectivamente, predominando a infecção por A. platys (p<0,001). Constatou-se um maior número de cães positivos para A. platys no período do inverno (p<0.05). Todas as amostras de sangue foram negativas para E. canis. Nos cães, a infestação por Amblyomma cajennense prevaleceu sobre a infestação por Rhipicephalus sanguineus (p<0,001), mas não foi observada associação significativa entre a PCR e as variáveis presença de carrapatos, sexo e idade. Cães infectados por A. platys e B. vogeli apresentaram trombocitopenia, linfopenia e leucocitose, porém não houve correlação destas alterações hematológicas com a infecção pelos hemoparasitas. Este é o primeiro estudo molecular que demonstra a existência de A. platys e de B. vogeli em cães da região sudoeste paranaense.(AU)