Beneficial Impact of Hypercapnic Conditions During Early Incubation on Broiler Hatchability. Embryo Mortality and Postnatal Performance

We assessed the extent to which CO2 levels altered different hatching and chick parameters. In Experiments 1 and 2, a total of 16,184 eggs from Cobb 500 breeders were incubated in single stage incubators under three different conditions: (a) standard ventilated incubator (CON, Exp.1 and 2); (b) increasing CO2 levels during the first 10 days of incubation until 0.7% (V7000, Exp. 1) and (c) until 0.8% (V8000, Exp. 2). High levels of CO2 improved hatchability, possibly due to lower embryo mortality from ED18 to ED21. Internal and external pipping in experiment V8000 started later than in CON; nevertheless, the hatch still occurred before in V8000 as a result of the shorter durations of external pipping and hatch. In Experiment 3, a total of 12,138 eggs from Cobb 500 were incubated in single stage incubators under three different conditions: (a) standard ventilated incubator (CON); (b) increasing CO2 levels until 1.0% with ventilation (V10000); and (c) increasing CO2 levels until 1.0% without ventilation (NV10000). Hypercapnic conditions led to better hatchability and lower embryo mortality from ED18 to ED21. Internal pipping started earlier in NV10000, but only V10000 differed from CON in terms of the average time for hatch. Hypercapnic groups also showed shorter durations of external pipping and hatch when compared to CON. Post-hatch analysis revealed no differences among incubation conditions in terms of body weight gain, feed conversion ratio, mortality by sudden death syndrome, and production factor. Nevertheless, V10000 showed a lower mortality by ascites and a better viability when compared to CON, while NV10000 presented a higher mortality by other causes. Altogether, our findings indicate that in addition to not being detrimental to embryo survival, high CO2 levels reduce embryonic mortality at 18-21 days of incubation and increase hatchability.(AU)

Effect of Dietary Growth Promoter Supplementations on the Serum Biochemistry and Morphometric Characteristics of Tibia Bone in Broiler Chicken

Bone mineralization is considered an essential source of minerals for metabolic needs that provide strength and hardness to the bone tissues and in birds its development can be enhanced by the use of dietary supplements. The present study was executed to evaluate the influence of prebiotic, probiotic and antibiotic on the morphometric aspects of tibia bone in broilers. One-hundred and sixty-one-day-old broiler chick were divided randomly into four groups (n=40) with 4 replicates (n=10 in each replicate). Group-I fed with only corn based basal diet and served as controlled group. Group-II with antibiotic supplement (Zinc bacitracin 0.04%), Group-III with probiotic supplement (lactobacillus 0.1%) & Group-IV with prebiotic supplement (mannan oligosaccharide 0.1%). On the 35th day, two birds were selected randomly from each replicate and slaughtered to record the morphometric parameters of both right and left tibia bones from each bird. The findings showed that the length, weight, thickness of lateral and medial wall, tibiotarsal index of bone and bone ash percentage of supplemented groups have had significant (p<0.05) difference when compared to that of the controlled group. The weight/length index in birds supplemented with prebiotic and probiotic was significantly higher (p<0.05) than in the antibiotic supplemented and control group. Medullary canal diameter of all the supplemented groups was significantly lower (p<0.05) than the control group. No significant (p>0.05) difference of robusticity index and diaphysis diameter was observed among various groups. A significant (p<0.05) low level of Alkaline Phosphatase while higher level of Calcium and Phosphorus was recorded in the supplemented groups compared to that of the control group. In conclusion, the use of prebiotic and probiotic as growth promoter can exhibit some underline mechanisms in the form of enhancement of bone quality, density and characteristics in broiler chicks as compared to common antibiotics.(AU)

In ovo feeding: a review / Alimentação in ovo: uma revisão

The poultry industry plays a large role in the Brazilian economy. In fact,Brazil ranks as the worlds leading exporter and second largest producer ofpoultry meat as the result of genetic improvement, animal nutrition,management, and biosecurity. During the development of bird embryos, thenutrients are stored within the egg, but carbohydrate supplementation isunsatisfactory upon hatching. Poor carbohydrate supplementation candamage the embryo development and negatively affect poultry weight. Inovo feeding is a promising technique to improve the quality of newly hatchedchicks as it uses systems for feeding the embryos by inoculating nutrientsinto the egg amniotic fluid. However, further studies are needed in order toimprove this technique and create effective implementations and protocolsthat can be widely used in the industry. This study aims to both review theliterature on the use of in-ovo feeding and understand the perspectives ofits use in the broiler production chain.

A avicultura tem tido papel expressivo na economia brasileira, estando o país classificado como o segundo maior produtor e o maior exportador do mundo. Esse resultado é proveniente de avanços no melhoramento genético, nutrição, manejo e biosseguridade. Nas aves, os nutrientes utilizados durante o desenvolvimento embrionário estão contidos no ovo, sendo escassa a reserva de carboidratos logo após a eclosão do pintainho. Essa restrição pode causar prejuízos ao desenvolvimento embrionário, impactando negativamente no peso das aves. Diante disso, a nutrição in ovo mostra-se como uma técnica promissora, que visa prover nutrientes ao embrião por meio da inoculação de soluções com efeitos benéficos ao seu desenvolvimento. Contudo, são necessários mais estudos no intuito de aprimorar a técnica e criação de protocolos efetivos que possam ser amplamente utilizados pela indústria. Nesse contexto, o objetivo desta revisão de literatura foi abordar e depreender as perspectivas da utilização da técnica nutrição in ovo na cadeia produtiva de frangos de corte.

In ovo feeding: a review / Alimentação in ovo: uma revisão

The poultry industry plays a large role in the Brazilian economy. In fact,Brazil ranks as the worlds leading exporter and second largest producer ofpoultry meat as the result of genetic improvement, animal nutrition,management, and biosecurity. During the development of bird embryos, thenutrients are stored within the egg, but carbohydrate supplementation isunsatisfactory upon hatching. Poor carbohydrate supplementation candamage the embryo development and negatively affect poultry weight. Inovo feeding is a promising technique to improve the quality of newly hatchedchicks as it uses systems for feeding the embryos by inoculating nutrientsinto the egg amniotic fluid. However, further studies are needed in order toimprove this technique and create effective implementations and protocolsthat can be widely used in the industry. This study aims to both review theliterature on the use of in-ovo feeding and understand the perspectives ofits use in the broiler production chain.(AU)

A avicultura tem tido papel expressivo na economia brasileira, estando o país classificado como o segundo maior produtor e o maior exportador do mundo. Esse resultado é proveniente de avanços no melhoramento genético, nutrição, manejo e biosseguridade. Nas aves, os nutrientes utilizados durante o desenvolvimento embrionário estão contidos no ovo, sendo escassa a reserva de carboidratos logo após a eclosão do pintainho. Essa restrição pode causar prejuízos ao desenvolvimento embrionário, impactando negativamente no peso das aves. Diante disso, a nutrição in ovo mostra-se como uma técnica promissora, que visa prover nutrientes ao embrião por meio da inoculação de soluções com efeitos benéficos ao seu desenvolvimento. Contudo, são necessários mais estudos no intuito de aprimorar a técnica e criação de protocolos efetivos que possam ser amplamente utilizados pela indústria. Nesse contexto, o objetivo desta revisão de literatura foi abordar e depreender as perspectivas da utilização da técnica nutrição in ovo na cadeia produtiva de frangos de corte.(AU)

Development of copper hatch baskets and evaluation of the productive indexes of a commercial hatchery

Background: The dissemination of pathogenic microorganisms in hatcheries leads to a higher number of contaminated eggs, causing reduction in hatchability and increase of discarded chicks. Sanitation programs are crucial for maximum hatchability and chick quality. Efforts have been made to find alternative approaches to the conventional disinfectants, and surfaces with copper, which have antimicrobial properties, could assist in this process. However, the possible adverse effects of copper surfaces on chicks in hatcheries have not yet been evaluated. The present study aimed at developing hatch baskets composed of copper and evaluating the effect of these baskets on the productive indexes of a hatchery. Materials, Methods & Results: For this experiment, 3.15 kg hatch tray prototypes with 99.9% Cu (Cu11000) were developed to fit inside conventional polypropylene hatch baskets (580 × 755 × 83 mm). Six polypropylene hatch baskets (control group) and six polypropylene hatch baskets covered by 99.9% copper (Cu11000) hatch trays (test group) were evaluated during 5 hatchings. Hatched eggs and chicks remained in contact with the hatch basket surfaces for at least 72 h, corresponding to the entire period in which they were located in the hatcher. Cleaning and disinfection programs of the hatchery were not modified. The level of microbial contamination on the hatch baskets was evaluated at 6 different periods: 0 h (initial contamination after disinfection and egg transfer to the trays); 24 h, 30 h, 45 h and 60 h after the first sampling; and at the moment when chicks were removed from the hatching cabinet and transferred to the chick-holding room (> 60 h). Counting of total moulds and yeasts, mesophilic microorganisms, Enterobacteria and Escherichia coli colonies was performed. The number of hatched chicks, non-hatched eggs, and chicks discarded were registered for each hatching. Microbiologic analyses showed no growth on hatch baskets neither of the..

Evaluation of the Relationship Between the Breeders Age, Breed and the Incubator Types on Quality and Animal Welfare in the Hatchery

About 33.3% of chicken life is conditioned by the result of embryonic development. Therefore, understanding and improving the early phase of the broilers life is critical to guarantee their well-being. Thus, the objective of this research was to evaluate the relationship between the breeders age, breed and the incubator types on quality and animal welfare in the hatchery. The experimental design was completely randomized in a 2 x 2 x 3 factorial arrangement (incubator, breed and age), with 12 treatments and 6 replicates each. The eggs derived of Cobb 500 and Rosss breeders at the beginning of the laying period, middle age and at the end of the laying period. It is concluded that the size of the eggs is related to the breeders age and breed, which influences directly the number of bled eggs. In addition, well-being in the hatchery can be affected by decreased hatching and contamination that is higher in older breeders. Cobb eggs also need more attention because they are larger, therefore, they have a higher risk of breakage and they are more sensitive to the low concentration of CO2 at the hatcher.(AU)

Evaluation of the Relationship Between the Breeders Age, Breed and the Incubator Types on Quality and Animal Welfare in the Hatchery

About 33.3% of chicken life is conditioned by the result of embryonic development. Therefore, understanding and improving the early phase of the broilers life is critical to guarantee their well-being. Thus, the objective of this research was to evaluate the relationship between the breeders age, breed and the incubator types on quality and animal welfare in the hatchery. The experimental design was completely randomized in a 2 x 2 x 3 factorial arrangement (incubator, breed and age), with 12 treatments and 6 replicates each. The eggs derived of Cobb 500 and Rosss breeders at the beginning of the laying period, middle age and at the end of the laying period. It is concluded that the size of the eggs is related to the breeders age and breed, which influences directly the number of bled eggs. In addition, well-being in the hatchery can be affected by decreased hatching and contamination that is higher in older breeders. Cobb eggs also need more attention because they are larger, therefore, they have a higher risk of breakage and they are more sensitive to the low concentration of CO2 at the hatcher.

Is the eggshell quality influenced by the egg weight or the breeder age?

For a long time, it has been said that eggshell quality decreases as eggs increase in size, but this increase is seen as the breeder age advances. Therefore, the present study aimed to evaluate the age and egg weight effect on Cobb 500 broiler breeders on eggshell quality. The quality measures tested were eggshell and mineral matter percentage, eggshell surface, shape and eggshell index, eggshell and membrane thickness and specific gravity. Eggs from 27, 31, 36, 40, 46, 48, 53, 58 and 63 week-old breeders were classified as eggs with a constant weight ranging from 63.8 ± 0.2; and eggs with increasing weight, according to age, being: 55.8g; 60g; 62.2g; 64.2g; 65.2g, 66.6g, 68.1g, 69.9g and 72.5g, respectively. The eggs weight did not influence the eggshell percentage and the specific gravity in seven of the nine tested ages, while for mineral matter in six of the nine studied ages. The shape and shell index, shell and membrane thickness are characteristics independent of egg weight. As the Cobb 500 broiler breeder age advanced, the mineral matter concentration and specific gravity maintained constant, the membrane thickness (R2>70) reduced, and other studied variables were influenced in the same way for both treatments. Thus, the eggshell quality decrease with the advancing age cannot be attributed solely to the increase of egg weight with consequent increase in the eggshell volume and the surface, but there are also age-related factors.(AU)

Is the eggshell quality influenced by the egg weight or the breeder age?

For a long time, it has been said that eggshell quality decreases as eggs increase in size, but this increase is seen as the breeder age advances. Therefore, the present study aimed to evaluate the age and egg weight effect on Cobb 500 broiler breeders on eggshell quality. The quality measures tested were eggshell and mineral matter percentage, eggshell surface, shape and eggshell index, eggshell and membrane thickness and specific gravity. Eggs from 27, 31, 36, 40, 46, 48, 53, 58 and 63 week-old breeders were classified as eggs with a constant weight ranging from 63.8 ± 0.2; and eggs with increasing weight, according to age, being: 55.8g; 60g; 62.2g; 64.2g; 65.2g, 66.6g, 68.1g, 69.9g and 72.5g, respectively. The eggs weight did not influence the eggshell percentage and the specific gravity in seven of the nine tested ages, while for mineral matter in six of the nine studied ages. The shape and shell index, shell and membrane thickness are characteristics independent of egg weight. As the Cobb 500 broiler breeder age advanced, the mineral matter concentration and specific gravity maintained constant, the membrane thickness (R2>70) reduced, and other studied variables were influenced in the same way for both treatments. Thus, the eggshell quality decrease with the advancing age cannot be attributed solely to the increase of egg weight with consequent increase in the eggshell volume and the surface, but there are also age-related factors.

Embryonic mortality and broiler chick quality (Gallus gallus) from glass-shelled eggs / Mortalidade embrionária e qualidade do pinto ao nascer oriundo de ovo de casca vítrea de matriz pesada (Gallus gallus)

The glass-shelled egg has various points on the surface with light gray coloration, which becomes more visible with the passage of egg storage period. The objective of this work was to evaluate the stage of embryonic mortality, egg weight during the incubation period, hatch window, and quality of chicks at hatch from glass-shelled egg. The research was divided into two parts. In the first part, 80 eggs from 45 weeks old hens were incubated, being half normal eggs and half glass-shelled eggs, while in the second part, 80 eggs from 70 weeks old hens from the same flock as the previous one were incubated. Again, half were normal shelled and half were glass-shelled eggs. No differences were observed for embryonic mortality, egg weight, hatch quality (hatch weight, uniformity, and navel quality), and hatch window between normal and glass-shelled eggs from hens of 45 and 70 weeks of age. However, there was higher contamination in glass-shelled egg from 70-week-old breeder hens. The results show that the presence of glass-shelled egg does not seem to interfere with the quality of the chick at hatch in the breed, ages, and studied conditions, but causes higher contamination in glass-shelled egg from 70-week-old breeder hens.

Ovos de casca vítrea apresentam em suas superfícies pontos com coloração cinza claro de vários diâmetros, que ficam mais visíveis com o decorrer do período de armazenagem. Objetivou-se no trabalho, avaliar a fase de mortalidade embrionária, peso do ovo durante o período de incubação, intervalo de tempo de nascimento e a qualidade dos pintos ao nascerem oriundos de ovos de casca vítrea. A pesquisa foi dividida em duas partes. Na primeira parte, ocorreu a incubação de 80 ovos provenientes de matrizes com 45 semanas de idade sendo metade ovos normais e metade ovos vítreos e na segunda parte, foram incubados 80 ovos provenientes de matrizes com 70 semanas de idade do mesmo lote do anterior. Desses, metade eram normais e metade eram vítreos. Não houve diferença para mortalidade embrionária, peso do ovo, qualidade dos pintos ao nascerem (peso do pinto ao nascer, qualidade do umbigo e uniformidade) e intervalo de tempo de nascimento entre os ovos vítreos e normais oriundos de matrizes de 45 e 70 semanas de idade. Porém ovos de casca vítrea oriundos de matrizes de 70 semanas de idade apresentaram maior índice de contaminação.

Embryonic mortality and broiler chick quality (Gallus gallus) from glass-shelled eggs / Mortalidade embrionária e qualidade do pinto ao nascer oriundo de ovo de casca vítrea de matriz pesada (Gallus gallus)

The glass-shelled egg has various points on the surface with light gray coloration, which becomes more visible with the passage of egg storage period. The objective of this work was to evaluate the stage of embryonic mortality, egg weight during the incubation period, hatch window, and quality of chicks at hatch from glass-shelled egg. The research was divided into two parts. In the first part, 80 eggs from 45 weeks old hens were incubated, being half normal eggs and half glass-shelled eggs, while in the second part, 80 eggs from 70 weeks old hens from the same flock as the previous one were incubated. Again, half were normal shelled and half were glass-shelled eggs. No differences were observed for embryonic mortality, egg weight, hatch quality (hatch weight, uniformity, and navel quality), and hatch window between normal and glass-shelled eggs from hens of 45 and 70 weeks of age. However, there was higher contamination in glass-shelled egg from 70-week-old breeder hens. The results show that the presence of glass-shelled egg does not seem to interfere with the quality of the chick at hatch in the breed, ages, and studied conditions, but causes higher contamination in glass-shelled egg from 70-week-old breeder hens.(AU)

Ovos de casca vítrea apresentam em suas superfícies pontos com coloração cinza claro de vários diâmetros, que ficam mais visíveis com o decorrer do período de armazenagem. Objetivou-se no trabalho, avaliar a fase de mortalidade embrionária, peso do ovo durante o período de incubação, intervalo de tempo de nascimento e a qualidade dos pintos ao nascerem oriundos de ovos de casca vítrea. A pesquisa foi dividida em duas partes. Na primeira parte, ocorreu a incubação de 80 ovos provenientes de matrizes com 45 semanas de idade sendo metade ovos normais e metade ovos vítreos e na segunda parte, foram incubados 80 ovos provenientes de matrizes com 70 semanas de idade do mesmo lote do anterior. Desses, metade eram normais e metade eram vítreos. Não houve diferença para mortalidade embrionária, peso do ovo, qualidade dos pintos ao nascerem (peso do pinto ao nascer, qualidade do umbigo e uniformidade) e intervalo de tempo de nascimento entre os ovos vítreos e normais oriundos de matrizes de 45 e 70 semanas de idade. Porém ovos de casca vítrea oriundos de matrizes de 70 semanas de idade apresentaram maior índice de contaminação.(AU)

Nutrição precoce de pintos de corte / Early nutrition of broiler chicks

Devido à precocidade e a grande capacidade de ganho de peso dos frangos de corte, a qualidade do pintinho se tornou um parâmetro fundamental. Pintos de baixa qualidade (refugos) tendem a ganhar menos peso e obter pior conversão alimentar, gerando prejuízo aos avicultores. Dentre os vários fatores que impactam na qualidade de pintos de corte, o jejum pós-eclosão é responsável por significativas perdas iniciais de desempenho, sendo essas, muitas vezes, não compensadas futuramente. Durante a vida embrionária, os pintos de corte já possuem enzimas digestivas ativas, assim como mecanismos de absorção de nutrientes no intestino; mas, tanto as enzimas, quanto a capacidade intestinal não se encontram no seu ápice de funcionamento. Após a eclosão, devido à falta de alimento, uma escassez de carboidratos de origem alimentar, leva ao pintinho necessitar prover glicose via gliconeogênese de origem proteica, processo esse que pode levar o pintainho à cetose e também redução da disponibilidade de água metabólica; sendo todo esse quadro agravado, quanto maior for o tempo em que a ave ficar em jejum, após o nascimento. O jejum pré-alojamento pode gerar inúmeros impactos negativos no desempenho da ave: no desenvolvimento muscular, no crescimento visceral e na microbiota intestinal. O fornecimento de uma ração logo após a eclosão, durante o trajeto incubatório-granja, se mostra uma alternativa para reduzir os malefícios ocorridos devido ao jejum pós-eclosão; no entanto, é necessário antes um estudo econômico, visto que dependendo do tempo de jejum entre o nascimento e o alojamento das aves essa prática pode não ser viável.

Due to the earliness and the large weight gain capacity of broiler chick quality has become a very important parameter. Low quality chicks (scrap) tend to gain less weight and get worse feed conversion, generating damage to poultry farmers. Among the many factors that impact the quality of broiler chicks, the post-hatching fasting is responsible for significant initial losses of performance, and these often not compensated in the future. During embryonic life, the broilers already have their active digestive enzymes, as well as nutrient uptake mechanisms in the gut, but both enzymes as the intestinal ability are not in their working apex. After hatching, due to lack of food, shortage of food-borne carbohydrates leads to chick need to provide glucose via protein source gluconeogenesis, a process that can take the chick to ketosis and also reducing the availability of metabolic water and all that table exacerbated the longer the time that the bird to fast after birth. The pre-accommodation fasting can generate numerous negative impacts on bird performance: in muscle development, visceral growth and intestinal microbiota. Providing a feed immediately after hatching in the hatchery-farm path shown an alternative to reduce the harm occurred because the post-hatching fasting, however is required before an economic study, since depending on the time of fasting between birth and the housing of birds this practice may not be feasible.

Nutrição precoce de pintos de corte / Early nutrition of broiler chicks

Devido à precocidade e a grande capacidade de ganho de peso dos frangos de corte, a qualidade do pintinho se tornou um parâmetro fundamental. Pintos de baixa qualidade (refugos) tendem a ganhar menos peso e obter pior conversão alimentar, gerando prejuízo aos avicultores. Dentre os vários fatores que impactam na qualidade de pintos de corte, o jejum pós-eclosão é responsável por significativas perdas iniciais de desempenho, sendo essas, muitas vezes, não compensadas futuramente. Durante a vida embrionária, os pintos de corte já possuem enzimas digestivas ativas, assim como mecanismos de absorção de nutrientes no intestino; mas, tanto as enzimas, quanto a capacidade intestinal não se encontram no seu ápice de funcionamento. Após a eclosão, devido à falta de alimento, uma escassez de carboidratos de origem alimentar, leva ao pintinho necessitar prover glicose via gliconeogênese de origem proteica, processo esse que pode levar o pintainho à cetose e também redução da disponibilidade de água metabólica; sendo todo esse quadro agravado, quanto maior for o tempo em que a ave ficar em jejum, após o nascimento. O jejum pré-alojamento pode gerar inúmeros impactos negativos no desempenho da ave: no desenvolvimento muscular, no crescimento visceral e na microbiota intestinal. O fornecimento de uma ração logo após a eclosão, durante o trajeto incubatório-granja, se mostra uma alternativa para reduzir os malefícios ocorridos devido ao jejum pós-eclosão; no entanto, é necessário antes um estudo econômico, visto que dependendo do tempo de jejum entre o nascimento e o alojamento das aves essa prática pode não ser viável.(AU)

Due to the earliness and the large weight gain capacity of broiler chick quality has become a very important parameter. Low quality chicks (scrap) tend to gain less weight and get worse feed conversion, generating damage to poultry farmers. Among the many factors that impact the quality of broiler chicks, the post-hatching fasting is responsible for significant initial losses of performance, and these often not compensated in the future. During embryonic life, the broilers already have their active digestive enzymes, as well as nutrient uptake mechanisms in the gut, but both enzymes as the intestinal ability are not in their working apex. After hatching, due to lack of food, shortage of food-borne carbohydrates leads to chick need to provide glucose via protein source gluconeogenesis, a process that can take the chick to ketosis and also reducing the availability of metabolic water and all that table exacerbated the longer the time that the bird to fast after birth. The pre-accommodation fasting can generate numerous negative impacts on bird performance: in muscle development, visceral growth and intestinal microbiota. Providing a feed immediately after hatching in the hatchery-farm path shown an alternative to reduce the harm occurred because the post-hatching fasting, however is required before an economic study, since depending on the time of fasting between birth and the housing of birds this practice may not be feasible.(AU)

HISTOMORFOMETRIA DE ORGÃOS LINFOIDES E DESENVOLVIMENTO INTESTINAL DE PINTOS DE CORTE ORIGINADOS DE MATRIZES COM DIFERENTES IDADES E SUBMETIDOS AO ESTRESSE POR CALOR NA INCUBAÇÃO

Abstract: This study was carried out to evaluate the effect of breeder age and hatching temperature variations on chick quality. The experiment was a completely randomized design in a 3x2 factorial arrangement (three breeder ages and two hatching temperatures). We used 720 eggs from three breeder ages (30, 42, or 64 weeks). The hatching temperature for control group was 37.5 ºC during all the hatching period and for the elevated temperature group, it was 39.0 ºC during 6h between 10 and 18 days of incubation. Five chicks per treatment were euthanized by cervical dislocation for bursal and spleen morphological measurements. The data were analyzed using ANOVA and the differences among means were compared by Tukey test (5%). Chicks from older flock had larger follicular area of bursa de Fabricius when hatched under normal temperature. The higher hatching temperature induced intestinal development because chicks hatched under high temperature had bigger crypt depth. Changes in hatching machine temperature affected young flock chick quality. Chicks from older flocks had better immune response.

Resumo: Objetivou-se avaliar o efeito da idade da matriz e da variação da temperatura de incubação sobre a qualidade do pinto neonato. O delineamento foi inteiramente casualizado em esquema fatorial 3x2 (três idades x duas temperaturas de incubação). Foram incubados 720 ovos, provenientes de matrizes com diferentes idades (30, 42 ou 64 semanas). A temperatura da máquina de incubar para os grupos controle foi mantida constante (37,5 ºC) e para os tratamentos com estresse por calor foi aplicada uma temperatura de 39,0 ºC por 6h durante o período de 10 a 18 dias de incubação. Cinco pintos por tratamento foram sacrificados para coletas de órgãos e realizada a histomorfometria intestinal, de baço e bursa. Os dados foram submetidos a análise de variância e foi utilizado o teste de Tukey (5%). A área dos folículos da bursa foi maior nos pintos oriundos de matrizes mais velhas quando os ovos foram incubados em temperatura ideal. A temperatura de 39,0 ºC estimulou o desenvolvimento intestinal. A variação na temperatura de incubação prejudicou a qualidade de pintos, sobretudo para pintos originados de matrizes jovens. Pintos oriundos de matrizes velhas apresentaram melhor resposta imune e desenvolvimento intestinal, independentemente da variação da temperatura de incubação.

Óleo de soja e sebo bovino na ração de poedeiras semipesadas criadas em regiões de clima quente / Soybean oil and beef tallow in the diet of semi-heavy laying hens reared in hot climate regions

The objective of this study was to evaluate the productive performance and physical quality of eggs from semi-heavy laying hens reared in hot climate regions and fed diets containing soybean oil or beef tallow. The experiment had a duration of 63 days divided into three cycles of 21 days each. A total of 160 semi-heavy Hisex Brown laying hens at 50 weeks of age and with an initial weight of 1.755 ± 0.172 kg were used. The birds were reared in a floor system and housed in boxes. A completely randomized design consisting of two treatments and five replicates was used, with 16 birds per experimental unit (box). Two experimental diets based on corn and soybean meal were formulated and soybean oil or beef tallow was added, corresponding to treatments 1 and 2, respectively. The following parameters were evaluated: egg production (%), feed intake (g/bird/day), egg mass (g/bird/day), feed conversion per egg mass (kg/kg), feed conversion per dozen eggs (kg/dozen), egg weight, percentage of yolk, egg white and shell (%), specific gravity (g/cm3), animal viability (%), and body weight variation (g). No differences (P>0.05) were observed in any of the parameters studied. The dietary inclusion of soybean oil or beef tallow does not influence the productive performance or egg quality of semi-heavy laying hens reared in hot climate regions.

O objetivo do estudo foi avaliar o desempenho produtivo e qualidade física dos ovos de poedeiras semipesadas criadas em regiões de clima quente, alimentadas com rações contendo óleo de soja ou sebo bovino. O período experimental teve duração de 63 dias, sendo dividido em três ciclos de 21 dias cada. Foram utilizadas 160 poedeiras semipesadas da linhagem Hisex Brown, com 50 semanas de idade e peso inicial de 1,755 ± 0,172 kg. As aves foram criadas em sistema de piso, sendo alojadas em boxes. O delineamento utilizado foi o inteiramente casualizado, composto por dois tratamentos e cinco repetições, com 16 aves por unidade experimental (boxe). Foram formuladas duas dietas experimentais a base de milho e farelo de soja, com a inclusão de óleo de soja e sebo bovino constituindo os tratamentos um e dois respectivamente. Os parâmetros avaliados foram: produção de ovos (%), consumo de ração (g/ave/dia), massa de ovos (g/ave/dia), conversão alimentar por massa de ovos (kg/kg), conversão alimentar por dúzia de ovos (kg/dúzia), peso dos ovos, porcentagem da gema, albúmen e casca (%), gravidade específica (g/cm3), viabilidade das aves (%) e variação do peso corporal (g). Não foram observadas diferenças (P>0,05) em nenhum dos parâmetros avaliados. O uso de óleo de soja ou sebo bovino na ração, não influencia o desempenho produtivo e qualidade dos ovos de poedeiras semipesadas, criadas em regiões de clima quente.

Óleo de soja e sebo bovino na ração de poedeiras semipesadas criadas em regiões de clima quente / Soybean oil and beef tallow in the diet of semi-heavy laying hens reared in hot climate regions

The objective of this study was to evaluate the productive performance and physical quality of eggs from semi-heavy laying hens reared in hot climate regions and fed diets containing soybean oil or beef tallow. The experiment had a duration of 63 days divided into three cycles of 21 days each. A total of 160 semi-heavy Hisex Brown laying hens at 50 weeks of age and with an initial weight of 1.755 ± 0.172 kg were used. The birds were reared in a floor system and housed in boxes. A completely randomized design consisting of two treatments and five replicates was used, with 16 birds per experimental unit (box). Two experimental diets based on corn and soybean meal were formulated and soybean oil or beef tallow was added, corresponding to treatments 1 and 2, respectively. The following parameters were evaluated: egg production (%), feed intake (g/bird/day), egg mass (g/bird/day), feed conversion per egg mass (kg/kg), feed conversion per dozen eggs (kg/dozen), egg weight, percentage of yolk, egg white and shell (%), specific gravity (g/cm3), animal viability (%), and body weight variation (g). No differences (P>0.05) were observed in any of the parameters studied. The dietary inclusion of soybean oil or beef tallow does not influence the productive performance or egg quality of semi-heavy laying hens reared in hot climate regions.(AU)

O objetivo do estudo foi avaliar o desempenho produtivo e qualidade física dos ovos de poedeiras semipesadas criadas em regiões de clima quente, alimentadas com rações contendo óleo de soja ou sebo bovino. O período experimental teve duração de 63 dias, sendo dividido em três ciclos de 21 dias cada. Foram utilizadas 160 poedeiras semipesadas da linhagem Hisex Brown, com 50 semanas de idade e peso inicial de 1,755 ± 0,172 kg. As aves foram criadas em sistema de piso, sendo alojadas em boxes. O delineamento utilizado foi o inteiramente casualizado, composto por dois tratamentos e cinco repetições, com 16 aves por unidade experimental (boxe). Foram formuladas duas dietas experimentais a base de milho e farelo de soja, com a inclusão de óleo de soja e sebo bovino constituindo os tratamentos um e dois respectivamente. Os parâmetros avaliados foram: produção de ovos (%), consumo de ração (g/ave/dia), massa de ovos (g/ave/dia), conversão alimentar por massa de ovos (kg/kg), conversão alimentar por dúzia de ovos (kg/dúzia), peso dos ovos, porcentagem da gema, albúmen e casca (%), gravidade específica (g/cm3), viabilidade das aves (%) e variação do peso corporal (g). Não foram observadas diferenças (P>0,05) em nenhum dos parâmetros avaliados. O uso de óleo de soja ou sebo bovino na ração, não influencia o desempenho produtivo e qualidade dos ovos de poedeiras semipesadas, criadas em regiões de clima quente. (AU)

Effect of Sources and Levels of Dietary Zinc on the Performance, Carcass Traits and Blood Parameters of Broilers

ABSTRACT A total of 400 one-day-old, straight-run, commercial (Ross 308) broiler chicks were used to evaluate the effects of two dietary levels of zinc (Zn) sources on broiler chick performance, carcass traits and blood parameters. Corn-soybean diets were formulated for three rearing phases (starter, grower and finisher). The two dietary treatments applied consisted of the addition per kg of diet of 80mg of inorganic Zn (ZnO) (T1), or 80mg of ZnO plus 42mg of an organic Zn-amino acid complex (Availa-Zn120; Zinpro Corporation, Eden Prairie, MN, USA), totaling 122mg of the combined organic and inorganic Zn sources (T2). Birds were distributed according to a completely randomized design in the two treatments with eight replicates (pens) of 25 birds each. Feed and water were provided ad libitum. On day 42, blood samples were taken from four birds closest to the group average weight per replicate (32 per treatment) and then slaughtered for carcass evaluation. The results of this study did not find any significant effect of either of the evaluated Zn sources on broiler growth performance. Mortality rate was significantly lower (p 0.05) by the higher Zn concentration and the Zn sources group (T2). Carcass yields were not significantly influenced by the treatments. Breast quality showed significant improvement (p 0.05) in shear force (T2), indicating juicier meat. Higher concentrations (p 0.05) of Zn, Phosphorus (P), and total protein in blood were noted in (T2). Birds fed a mixture of organic and inorganic Zn source (T2) presented overall better results.(AU)

Effect of Sources and Levels of Dietary Zinc on the Performance, Carcass Traits and Blood Parameters of Broilers

ABSTRACT A total of 400 one-day-old, straight-run, commercial (Ross 308) broiler chicks were used to evaluate the effects of two dietary levels of zinc (Zn) sources on broiler chick performance, carcass traits and blood parameters. Corn-soybean diets were formulated for three rearing phases (starter, grower and finisher). The two dietary treatments applied consisted of the addition per kg of diet of 80mg of inorganic Zn (ZnO) (T1), or 80mg of ZnO plus 42mg of an organic Zn-amino acid complex (Availa-Zn120; Zinpro Corporation, Eden Prairie, MN, USA), totaling 122mg of the combined organic and inorganic Zn sources (T2). Birds were distributed according to a completely randomized design in the two treatments with eight replicates (pens) of 25 birds each. Feed and water were provided ad libitum. On day 42, blood samples were taken from four birds closest to the group average weight per replicate (32 per treatment) and then slaughtered for carcass evaluation. The results of this study did not find any significant effect of either of the evaluated Zn sources on broiler growth performance. Mortality rate was significantly lower (p 0.05) by the higher Zn concentration and the Zn sources group (T2). Carcass yields were not significantly influenced by the treatments. Breast quality showed significant improvement (p 0.05) in shear force (T2), indicating juicier meat. Higher concentrations (p 0.05) of Zn, Phosphorus (P), and total protein in blood were noted in (T2). Birds fed a mixture of organic and inorganic Zn source (T2) presented overall better results.

Histomorfometria de orgãos linfoides e desenvolvimento intestinal de pintos de corte originados de matrizes com diferentes idades e submetidos ao estresse por calor na incubação / Morphologigal measurements of lymphoid tissues and intestinal development of chicks from different breeder ages and hatched under heat stress

This study was carried out to evaluate the effect of breeder age and hatching temperature variations on chick quality. The experiment was a completely randomized design in a 3x2 factorial arrangement (three breeder ages and two hatching temperatures). We used 720 eggs from three breeder ages (30, 42, or 64 weeks). The hatching temperature for control group was 37.5 C during all the hatching period and for the elevated temperature group, it was 39.0 C during 6h between 10 and 18 days of incubation. Five chicks per treatment were euthanized by cervical dislocation for bursal and spleen morphological measurements. The data were analyzed using ANOVA and the differences among means were compared by Tukey test (5%). Chicks from older flock had larger follicular area of bursa de Fabricius when hatched under normal temperature. The higher hatching temperature induced intestinal development because chicks hatched under high temperature had bigger crypt depth. Changes in hatching machine temperature affected young flock chick quality. Chicks from older flocks had better immune response.

Objetivou-se avaliar o efeito da idade da matriz e da variação da temperatura de incubação sobre a qualidade do pinto neonato. O delineamento foi inteiramente causalizado em esquema fatorial 3x2 (três idades x duas temperaturas de incubação). Foram incubados 720 ovos, provenientes de matrizes com diferentes idades (30, 42 ou 64 semanas). A temperatura da máquina de incubar para os grupos controle foi mantida constante (37,5 ºC) e para os tratamentos com estresse por calor foi aplicada uma temperatura de 39,0 ºC por 6h durante o período de 10 a 18 dias de incubação. Cinco pintos por tratamento foram sacrificados para coletas de órgãos e realizada a histomorfometria intestinal, de baço e bursa. Os dados foram submetidos a análise de variância e foi utilizado o teste de Tukey (5%). A área dos folículos da bursa foi maior nos pintos oriundos de matrizes mais velhas quando os ovos foram incubados em temperatura ideal. A temperatura de 39,0 ºC estimulou o desenvolvimento intestinal. A variação na temperatura de incubação prejudicou a qualidade de pintos, sobretudo para pintos originados de matrizes jovens. Pintos oriundos de matrizes velhas apresentaram melhor resposta imune e desenvolvimento intestinal, independentemente da variação da temperatura de incubação.

Histomorfometria de orgãos linfoides e desenvolvimento intestinal de pintos de corte originados de matrizes com diferentes idades e submetidos ao estresse por calor na incubação / Morphologigal measurements of lymphoid tissues and intestinal development of chicks from different breeder ages and hatched under heat stress

This study was carried out to evaluate the effect of breeder age and hatching temperature variations on chick quality. The experiment was a completely randomized design in a 3x2 factorial arrangement (three breeder ages and two hatching temperatures). We used 720 eggs from three breeder ages (30, 42, or 64 weeks). The hatching temperature for control group was 37.5 C during all the hatching period and for the elevated temperature group, it was 39.0 C during 6h between 10 and 18 days of incubation. Five chicks per treatment were euthanized by cervical dislocation for bursal and spleen morphological measurements. The data were analyzed using ANOVA and the differences among means were compared by Tukey test (5%). Chicks from older flock had larger follicular area of bursa de Fabricius when hatched under normal temperature. The higher hatching temperature induced intestinal development because chicks hatched under high temperature had bigger crypt depth. Changes in hatching machine temperature affected young flock chick quality. Chicks from older flocks had better immune response.(AU)

Objetivou-se avaliar o efeito da idade da matriz e da variação da temperatura de incubação sobre a qualidade do pinto neonato. O delineamento foi inteiramente causalizado em esquema fatorial 3x2 (três idades x duas temperaturas de incubação). Foram incubados 720 ovos, provenientes de matrizes com diferentes idades (30, 42 ou 64 semanas). A temperatura da máquina de incubar para os grupos controle foi mantida constante (37,5 ºC) e para os tratamentos com estresse por calor foi aplicada uma temperatura de 39,0 ºC por 6h durante o período de 10 a 18 dias de incubação. Cinco pintos por tratamento foram sacrificados para coletas de órgãos e realizada a histomorfometria intestinal, de baço e bursa. Os dados foram submetidos a análise de variância e foi utilizado o teste de Tukey (5%). A área dos folículos da bursa foi maior nos pintos oriundos de matrizes mais velhas quando os ovos foram incubados em temperatura ideal. A temperatura de 39,0 ºC estimulou o desenvolvimento intestinal. A variação na temperatura de incubação prejudicou a qualidade de pintos, sobretudo para pintos originados de matrizes jovens. Pintos oriundos de matrizes velhas apresentaram melhor resposta imune e desenvolvimento intestinal, independentemente da variação da temperatura de incubação.(AU)