Familywise type I error of ANOVA and ANOVA on ranks in factorial experiments / Erro tipo I familiar da ANOVA e da ANOVA on ranks em experimentos fatoriais

ABSTRACT: This research evaluated the importance of a preliminary general analysis of variance (ANOVA) in the interpretation of data from factorial experiments under total nullity. For this, we evaluated the familywise type I error rate (accumulated FWER) of the F test for the unfolding of factorial ANOVA and factorial ANOVA on ranks, which were compared with the FWER for the global effect of treatments. In addition, we evaluated the FWER of the Tukey's test under total nullity for factorial experiments in the presence or absence of preliminary ANOVA protection (omnibus F test). The study was conducted by simulating data from 2,000 experiments, which were separated into four representative agricultural research scenarios. For both the parametric factorial ANOVA and the non-parametric factorial ANOVA, the FWER significantly exceeded the nominal level of 5%, even under total nullity. While the tests that control the total FWER in the factorials are not being used, the factorial ANOVA should not be performed without the preliminary ANOVA F test showing a significant effect. This, of course, does not apply to tests that are not multiple comparisons, such as Bonferroni, Dunn-Sidak and others, which do not need ANOVA protection. The same recommendation applies to the factorial ANOVA on ranks.

RESUMO: Este trabalho teve como objetivo avaliar a importância da análise de variância (ANOVA) geral preliminar na interpretação dos dados de experimentos fatoriais sob nulidade total. Para isso, avaliou-se as taxas de erro tipo I familiar (FWER acumulada) do teste F para os componentes do desdobramento da ANOVA fatorial e da ANOVA fatorial on ranks, em comparação à FWER para o efeito global de tratamentos. Além disso, avaliou-se a FWER do teste de Tukey sob nulidade total para experimentos fatoriais na presença ou ausência da proteção da ANOVA preliminar (teste F global). O estudo foi conduzido a partir da simulação de dados de 2000 experimentos, separados em quatro cenários representativos da pesquisa agrícola. Tanto para a ANOVA fatorial paramétrica, quanto para a ANOVA fatorial não-paramétrica, as FWER ultrapassaram significativamente o nível nominal de 5%, mesmo sob nulidade total. Enquanto os testes que controlam a FWER total nos fatoriais não estiverem sendo utilizados, a ANOVA fatorial não deve ser realizada sem que o teste F da ANOVA preliminar acuse um efeito significativo. O mesmo, evidentemente, não se aplica aos testes que não são de comparações múltiplas, como Bonferroni, Dunn-Sidak e outros, que não precisam proteção da ANOVA. A mesma recomendação se aplica à ANOVA fatorial on ranks.

AgroReg: main regression models in agricultural sciences implemented as an R Package

Regression analysis is highly relevant to agricultural sciences since many of the factors studied are quantitative. Researchers have generally used polynomial models to explain their experimental results, mainly because much of the existing software perform this analysis and a lack of knowledge of other models. On the other hand, many of the natural phenomena do not present such behavior; nevertheless, the use of non-linear models is costly and requires advanced knowledge of language programming such as R. Thus, this work presents several regression models found in scientific studies, implementing them in the form of an R package called AgroReg. The package comprises 44 analysis functions with 66 regression models such as polynomial, non-parametric (loess), segmented, logistic, exponential, and logarithmic, among others. The functions provide the coefficient of determination (R2), model coefficients and the respective p-values from the t-test, root mean square error (RMSE), Akaike's information criterion (AIC), Bayesian information criterion (BIC), maximum and minimum predicted values, and the regression plot. Furthermore, other measures of model quality and graphical analysis of residuals are also included. The package can be downloaded from the CRAN repository using the command: install.packages("AgroReg"). AgroReg is a promising analysis tool in agricultural research on account of its user-friendly and straightforward functions that allow for fast and efficient data processing with greater reliability and relevant information.

Salmonella Serovars Isolated from Poultry Breeding Flocks under the Brazilian Official Control Programme Between 2016 and 2018

The control of Salmonella in the poultry production chain combined with biosecurity measures is an important tool to maintain and guarantee the sanitary status of Brazilian flocks. The aim of this work was to compare official laboratory data on molecular typification of Salmonella isolates from poultry breeding flocks in different Brazilian states between 2016 and 2018 and identify the production category with the most positive flocks, in light of current legislation. Surveillance data of positive samples from the official Brazilian Salmonella Control Programme sent to Federal Agricultural Defence Laboratory of São Paulo (LFDA-SP) after molecular characterization were analysed. These data were subject to an exploratory study, undergoing a descriptive statistical analysis followed by the use of frequency and non-parametric hypothesis tests. Overall, 49 serovars were detected in poultry broiler-breeder and layer-breeder flocks. Salmonella ser. Heidelberg, Salmonella ser. Anatum, Salmonella ser. Newport, Salmonella ser. Schwarzengrund and Salmonella ser. Mbandaka were the five most common isolated serovars. The data shows that there is an opportunity to improve biosecurity measures in parent breeder flocks. A total of 16 serovars were identified in turkey-breeders. Salmonella ser. Anatum, Salmonella ser. Newport, Salmonella ser. Brandenburg, Salmonella ser. Litchfield, and Salmonella ser. Livingstone were the most common ones. The four official controlled serovars represented a small part of the isolated strains. These data demonstrate the importance of an official program in Brazil for Salmonella surveillance in breeder flocks combined with biosecurity measures.(AU)

Replacing corn silage with extruded forage in sheep feeding

The objective was to evaluate levels of replacement of corn silage with extruded forage (Foragge®) in sheep feeding. Twenty adult Santa Inês ewes in maintenance, and 55.8 kg average weight were housed in metabolic cages, and the treatments consisted of 20, 40, 60 and 80% replacement of corn silage with Foragge®extruded forage with 60% Uruchloa brizanthain the diet. The design was completely randomized, with regression analysis and non-parametric analysis at 5% probability. The supply of up to 80% extruded roughage to replace corn silage increased dry matter intake and digestibility, and fecal output in dry matter (p < 0.05). Also, it increased the efficiency of ingestion, rumination and chewing activities of sheep, but did not alter urinary parameters and energy metabolites (p > 0.05). However, it reduced the concentration of uric acid (p < 0.05). Foragge®extruded roughage can replace corn silage by up to 80% with changes in intake, digestibility, fecal parameters, ingestive behavior and concentration of uric acid and urea, with no modifications in urinary parameters and energy metabolites.(AU)

Testicular morphometry as a tool to evaluate the efficiency of immunocastration in lambs

This study aimed to evaluate the efficiency of immunocastration in lambs using testicular morphometry. Thirty lambs were randomly divided into two treatments (subcutaneous administration of 1.0 mL and 0.5 mL of an anti-GnRH vaccine) and a control group (1.0 mL saline solution). The animals were vaccinated at four months of age, received a second dose 30 days later, and were slaughtered 90 days after the first vaccine dose. After slaughter, testicles were collected, and samples were removed for histological processing and evaluation of testicular morphometric parameters. Analysis of variance, Tukey's test, and Kruskal-Wallis test were performed, with a 5% level of significance. There was a reduction in testicular weight, gonadosomatic index, seminiferous tubule diameter, germinal epithelium height, leydigosomatic index, and total tubule length. The total length per testicular gram increased in the immunocastrated group. Intrinsic spermatogenesis yield, Sertoli cell indices, and estimates of sperm and Sertoli cell production were reduced in the immunized groups (P < 0.05). The anti-GnRH vaccine in lambs at doses of 1.0 mL and 0.5 mL is sufficient to promote immunocastration, verified through severe changes in testicular morphometry from animals.(AU)

Docosahexaenoic acid in diluent for goat semen cryopreservation

Abstract The effects of docosahexaenoic acid (DHA) in the diluent for cryopreservation of goat semen on seminal quality and the optimal levels to be used were evaluated. After collection, semen was pooled and physically evaluated, then divided into four aliquots with different DHA levels in the diluent: 0, 10, 20, and 30 ng mL-1. The semen was cryopreserved in a TK 3000® freezing machine and then thawed for assessment at 37 °C. Sperm motility and vigor, membrane integrity, acrosomal integrity, mitochondrial activity, and sperm chromatin compaction were evaluated after thawing. A completely randomized design was used. For normally distributed variables, ANOVA and regression analysis were used to test for differences between treatments, and for non-parametric data, the Kruskal Wallis test was used at the 5% significance level. There were no differences among groups in terms of membrane integrity, acrosomal integrity, or chromatin compaction. There was a decrease in class I mitochondrial activity with increasing DHA level (P<0.05), but no differences in classes II, III, and IV (P>0.05). The inclusion of 10 to 30 ng mL-1 of DHA in the diluent did not result in improvements in seminal quality parameters after thawing, with some impairment observed in the mitochondrial activity of the sperm cells.

Surface quality of furniture components using a band saw, milling machine, and two species / Qualidade superficial de componentes de móveis utilizando serra fita e fresadora e duas espécies

This study is dedicated to considering the use of eucalyptus and Ocotea porosa to produce classic-style furniture, assessing the machining time and surface quality. For such, the survey of data on the surface quality of eucalyptus and Ocotea porosa pieces was carried out through a visual assessment with the adaptation of the methodology described in standard ASTM 1666. This analysis of the wood surface quality occurred using two different species and two machining modalities (a band saw and a copy milling machine) for parts intended to produce components (feet) for classic furniture. After making the feet, the pieces of furniture were assembled and reassessed. Forty samples were selected and evaluated for initial defects through visual inspection, and the non-parametric Kruskal-Wallis statistical test was applied. The values found for surface quality were considered good quality, with more time being necessary to perform the finishing of the parts processed with the band saw than the milling machine. One may conclude thatthe milling time spent on each treatment influences the production capacity of the company, with the cuts carried out with the milling machine being faster and with better surface finishing.

Esse estudo dedicou-se a considerar a utilização de eucalipto e Ocotea porosa na produção de móveis em estilo clássico, avaliando o tempo de usinagem e a qualidade superficial. Para tal, foi realizado o levantamento de dados sobre a qualidade superficial de peças de eucalipto e Ocotea porosa, por meio de uma avaliação visual com adaptação da metodologia descrita na norma ASTM 1666. Essa análise da qualidade superficial da madeira ocorreu por meio de duas diferentes espécies e conduzido por duas modalidades de usinagem (uma serra-fita e uma fresadora copiadora) de peças destinadas a produção de componentes (pés) para móveis clássicos. Após a confecção dos pés os móveis foram montados e novamente avaliados. Selecionou-se 40 amostras, nas quais foram avaliados os defeitos iniciais por meioda inspeção visual, foi aplicada a estatística não paramétrica de Kruskal-Wallis. Os valores encontrados sobre a qualidade de superfície foram considerados de boa qualidade, sendo necessário maior tempo para realizar o acabamento nas peças processadas na serra-fita se comparado com a fresadora. Pôde-se concluir que o tempo de usinagem gasto em cada tratamento influencia na capacidade de produção da empresa, sendo mais rápidos os cortes realizados pela fresadora com melhor acabamento superficial.

Surface quality of furniture components using a band saw, milling machine, and two species / Qualidade superficial de componentes de móveis utilizando serra fita e fresadora e duas espécies

This study is dedicated to considering the use of eucalyptus and Ocotea porosa to produce classic-style furniture, assessing the machining time and surface quality. For such, the survey of data on the surface quality of eucalyptus and Ocotea porosa pieces was carried out through a visual assessment with the adaptation of the methodology described in standard ASTM 1666. This analysis of the wood surface quality occurred using two different species and two machining modalities (a band saw and a copy milling machine) for parts intended to produce components (feet) for classic furniture. After making the feet, the pieces of furniture were assembled and reassessed. Forty samples were selected and evaluated for initial defects through visual inspection, and the non-parametric Kruskal-Wallis statistical test was applied. The values found for surface quality were considered good quality, with more time being necessary to perform the finishing of the parts processed with the band saw than the milling machine. One may conclude thatthe milling time spent on each treatment influences the production capacity of the company, with the cuts carried out with the milling machine being faster and with better surface finishing.(AU)

Esse estudo dedicou-se a considerar a utilização de eucalipto e Ocotea porosa na produção de móveis em estilo clássico, avaliando o tempo de usinagem e a qualidade superficial. Para tal, foi realizado o levantamento de dados sobre a qualidade superficial de peças de eucalipto e Ocotea porosa, por meio de uma avaliação visual com adaptação da metodologia descrita na norma ASTM 1666. Essa análise da qualidade superficial da madeira ocorreu por meio de duas diferentes espécies e conduzido por duas modalidades de usinagem (uma serra-fita e uma fresadora copiadora) de peças destinadas a produção de componentes (pés) para móveis clássicos. Após a confecção dos pés os móveis foram montados e novamente avaliados. Selecionou-se 40 amostras, nas quais foram avaliados os defeitos iniciais por meioda inspeção visual, foi aplicada a estatística não paramétrica de Kruskal-Wallis. Os valores encontrados sobre a qualidade de superfície foram considerados de boa qualidade, sendo necessário maior tempo para realizar o acabamento nas peças processadas na serra-fita se comparado com a fresadora. Pôde-se concluir que o tempo de usinagem gasto em cada tratamento influencia na capacidade de produção da empresa, sendo mais rápidos os cortes realizados pela fresadora com melhor acabamento superficial.(AU)

Use of active and inactive yeasts in lamb diets: intake, digestibility, and metabolism / Uso de leveduras ativas e inativas na dieta de cordeiros: consumo, digestibilidade e metabolismo

This study aims to evaluate the use of active, inactive plus active, and inactive yeasts on the consumption of dry matter, water, and the apparent digestibility of dry matter, urinary parameters, and serum metabolites of lambs. Twenty crossbred lambs (Dorper x Santa Inês) were used, with an initial average body weight of 31.89 kg and seven months of age, distributed in a completely randomized design. Treatments were Control group (without yeasts); Active Flora® (ICC), live yeast plus inactivated yeast (Saccharomyces cerevisiae, 2.0 x 1010 UFC g-1) in the dose of 0.003 kg of animal dry matter -1 day-1,; Milk Sacc X® (Alltech®), active yeast - Saccharomyces cerevisiae strain 1026, 5.0 x 108 UFC g-1 - at the dose of 0.0015 kg of animal dry matter-1 day-1; and Rumen Yeast® (York Ag Products INC.), inactive yeast - Saccharomyces cerevisiae,1.5 x 104 UFC g-1 - in the dose of 0.0045 kg of animal dry matter-1 day-1. Variance analysis and the SNK (Student-Newman-Keuls) test were performed considering 5% significance. For glycemic concentrations over time, regression analysis at 5% significance were performed. The fecal score, as a non-parametric variable, was assessed by the Kruskal and Wallis test at a 5% significance level. There was a statistical difference (P 0.05). The average dry matter intake was 1.16 ± 0.16 kg day-1, whereas the dry matter digestibility was 85.40 ± 2.73%. It was concluded that active and/or inactive yeasts can be used as additives in the diet of lambs, maintaining the intake and digestibility of dry matter without causing metabolic disturbances.

Objetivou-se avaliar o efeito de leveduras ativas, inativas mais ativas e inativas sobre o consumo e digestibilidade aparente da matéria seca, de água, dos parâmetros urinários e dos metabólitos séricos de borregas. Utilizou-se 20 borregas mestiças Dorper x Santa Inês, com peso corporal médio inicial de 31,89 kg e sete meses de idade, distribuídas em delineamento inteiramente casualizado. Os tratamentos consistiram em Controle, sem uso de enzimas, e no uso das leveduras Active Flora® (levedura viva junto a levedura inativada - Saccharomyces Cerevisiae, com 2,0 x 1010 UFC g-1), Milk Sacc X® (levedura ativa - Saccharomyces C. 1026, 5,0 x 108 UFC g-1) e Rúmen Yeast® (levedura inativa - Saccharomyces C. com 1,5 x 104 UFC g-1). Foi realizado uma análise de variância e um teste SNK considerando 5% de significância. Para as concentrações glicêmicas ao longo do tempo foi realizada uma análise de regressão a 5% de significância. O escore fecal, por ser uma variável não paramétrica, foi avaliado pelo teste Kruskal e Wallis à significância de 5%. Observou-se a diferença estatística (P 0,05). A média de consumo da matéria seca foi de 1,16±0,16 kg dia-1, já a digestibilidade da matéria seca foi de 85,40 ±2,73%. Concluiu-se que as leveduras ativas e/ou inativas podem ser utilizadas como aditivos na dieta de borregas, mantendo o consumo e a digestibilidade da matéria seca sem causar distúrbios metabólicos.

Docosahexaenoic acid in diluent for goat semen cryopreservation

The effects of docosahexaenoic acid (DHA) in the diluent for cryopreservation of goat semen on seminal quality and the optimal levels to be used were evaluated. After collection, semen was pooled and physically evaluated, then divided into four aliquots with different DHA levels in the diluent: 0, 10, 20, and 30 ng mL-1. The semen was cryopreserved in a TK 3000® freezing machine and then thawed for assessment at 37 °C. Sperm motility and vigor, membrane integrity, acrosomal integrity, mitochondrial activity, and sperm chromatin compaction were evaluated after thawing. A completely randomized design was used. For normally distributed variables, ANOVA and regression analysis were used to test for differences between treatments, and for non-parametric data, the Kruskal Wallis test was used at the 5% significance level. There were no differences among groups in terms of membrane integrity, acrosomal integrity, or chromatin compaction. There was a decrease in class I mitochondrial activity with increasing DHA level (P<0.05), but no differences in classes II, III, and IV (P>0.05). The inclusion of 10 to 30 ng mL-1 of DHA in the diluent did not result in improvements in seminal quality parameters after thawing, with some impairment observed in the mitochondrial activity of the sperm cells.(AU)

Use of active and inactive yeasts in lamb diets: intake, digestibility, and metabolism / Uso de leveduras ativas e inativas na dieta de cordeiros: consumo, digestibilidade e metabolismo

This study aims to evaluate the use of active, inactive plus active, and inactive yeasts on the consumption of dry matter, water, and the apparent digestibility of dry matter, urinary parameters, and serum metabolites of lambs. Twenty crossbred lambs (Dorper x Santa Inês) were used, with an initial average body weight of 31.89 kg and seven months of age, distributed in a completely randomized design. Treatments were Control group (without yeasts); Active Flora® (ICC), live yeast plus inactivated yeast (Saccharomyces cerevisiae, 2.0 x 1010 UFC g-1) in the dose of 0.003 kg of animal dry matter -1 day-1,; Milk Sacc X® (Alltech®), active yeast - Saccharomyces cerevisiae strain 1026, 5.0 x 108 UFC g-1 - at the dose of 0.0015 kg of animal dry matter-1 day-1; and Rumen Yeast® (York Ag Products INC.), inactive yeast - Saccharomyces cerevisiae,1.5 x 104 UFC g-1 - in the dose of 0.0045 kg of animal dry matter-1 day-1. Variance analysis and the SNK (Student-Newman-Keuls) test were performed considering 5% significance. For glycemic concentrations over time, regression analysis at 5% significance were performed. The fecal score, as a non-parametric variable, was assessed by the Kruskal and Wallis test at a 5% significance level. There was a statistical difference (P< 0.05) in fecal dry matter (FDM), where the Active Flora® treatment was inferior to the others. The use of different yeasts did not change the intake and digestibility of dry matter, water, urinary parameters, and serum concentrations of energy, protein, and liver metabolites (P> 0.05). The average dry matter intake was 1.16 ± 0.16 kg day-1, whereas the dry matter digestibility was 85.40 ± 2.73%. It was concluded that active and/or inactive yeasts can be used as additives in the diet of lambs, maintaining the intake and digestibility of dry matter without causing metabolic disturbances.(AU)

Objetivou-se avaliar o efeito de leveduras ativas, inativas mais ativas e inativas sobre o consumo e digestibilidade aparente da matéria seca, de água, dos parâmetros urinários e dos metabólitos séricos de borregas. Utilizou-se 20 borregas mestiças Dorper x Santa Inês, com peso corporal médio inicial de 31,89 kg e sete meses de idade, distribuídas em delineamento inteiramente casualizado. Os tratamentos consistiram em Controle, sem uso de enzimas, e no uso das leveduras Active Flora® (levedura viva junto a levedura inativada - Saccharomyces Cerevisiae, com 2,0 x 1010 UFC g-1), Milk Sacc X® (levedura ativa - Saccharomyces C. 1026, 5,0 x 108 UFC g-1) e Rúmen Yeast® (levedura inativa - Saccharomyces C. com 1,5 x 104 UFC g-1). Foi realizado uma análise de variância e um teste SNK considerando 5% de significância. Para as concentrações glicêmicas ao longo do tempo foi realizada uma análise de regressão a 5% de significância. O escore fecal, por ser uma variável não paramétrica, foi avaliado pelo teste Kruskal e Wallis à significância de 5%. Observou-se a diferença estatística (P< 0,05) na matéria seca fecal (MSF), onde o tratamento Active Flora® se mostrou inferior aos demais. O uso de diferentes leveduras não modificou o consumo e a digestibilidade da matéria seca, de água, dos parâmetros urinários e das concentrações séricas dos metabólitos energéticos, proteicos e hepáticos (P> 0,05). A média de consumo da matéria seca foi de 1,16±0,16 kg dia-1, já a digestibilidade da matéria seca foi de 85,40 ±2,73%. Concluiu-se que as leveduras ativas e/ou inativas podem ser utilizadas como aditivos na dieta de borregas, mantendo o consumo e a digestibilidade da matéria seca sem causar distúrbios metabólicos.(AU)

Glutathione and IGF-1 in bovine seminal cryopreservation: oxidative stress response and pregnancy rate / [Glutationa e IGF-1 na criopreservação seminal bovina: resposta do estresse oxidativo e taxa de gestação]

The objective of this study was to evaluate the rate of conception, metabolic, and structural conditions of cryopreserved bovine sperm cells, plus extender with IGF-1 and glutathione (GSH). 12 ejaculations of Nelore bulls were used, submitted to treatments: control, gSH (2mM/mL), IGF-1 (100ng/mL) and gSH (1mM/mL) + IGF-1 (50ng/mL). After cryopreservation and thawing the semen passed the fast thermo resistance test (TTR), plasma membrane and acrosomal integrity (PIAI), mitochondrial membrane potential (AP), oxidative stress, and conception rate. Tukey test was used for the statistical analysis of the parametric variables and the Friedman test for nonparametric. The gestation percentage was compared by the Chi-square test. There was no statistical difference (P<0.05) between treatments for the TTRr variable. Otherwise in the oxidative stress evaluated with the CellROX probe was noted that the IGF-1 showed the highest number of reactive cells (P<0.05). The PIAI, AP and gestation rate showed no difference among treatments (P>0.05), with an average of conceptions of 36.58%. It is concluded that IGF-1, gSH and their association did not cause changes in sperm motility, mitochondrial potential, plasma and acrosomal membrane integrity. IGF-1 increased oxidative stress, however, there was no difference in the gestation rate among the treatments.(AU)

Objetivou-se avaliar a taxa de concepção, as condições metabólicas e estrutural das células espermáticas bovinas criopreservadas, acrescidas de diluidores com fator de crescimento semelhante à insulina do tipo 1(IGF-1) e glutationa (GSH). Foram utilizados 12 ejaculados de touros da raça Nelore, submetidos aos tratamentos: controle, gSH (2mM/mL), IGF-1 (100ng/mL) e gSH (1mM/mL) + IGF-1 (50ng/mL). Após a criopreservação e descongelação, o sêmen passou pelos testes de termorresistência rápida (TTRr), integridade de membrana plasmática e acrossomal (PIAI), alto potencial mitocondrial (AP), estresse oxidativo e taxa de concepção. Utilizou-se o teste de Tukey para as análises estatísticas das variáveis paramétricas e o teste de Friedman para as não paramétricas, com significância de 5%. A percentagem de gestação foi comparada pelo teste do qui-quadrado. Não hove diferença estatística (P<0,05) entre os tratamentos para a variável TTRr. Já no estresse oxidativo avaliado com a sonda CellROX, observou-se que o IGF-1 apresentou maior quantidade de células reativas (P<0,05), 36,38± 24,10. A PIAI, o AP e a taxa de gestação não apresentaram diferença entre tratamentos (P>0,05), com média de concepções de 36,58%. Conclui-se que o IGF-1, a gSH e a sua associação não causaram mudanças na motilidade espermática, no potencial mitocondrial, na integridade da membrana plasmática e acrossomal. O IGF-1 aumentou o estresse oxidativo, porém sem diferença na taxa de gestação entre os tratamentos.(AU)

A transversal study of biochemical profile, urinalysis, UPC, electrolytes and blood pressure in dogs with chronic kidney disease

Background: Chronic kidney disease (CKD) affects both dogs and cats, mainly elderly animals, due to tubulointerstitialinflammation associated with the increase of fibrosis through the excess deposition of extracellular matrix (ECM) whichleads to decrease glomerular filtration. Many different underlying renal diseases can affect the kidneys of dogs such ascongenital or acquired in origin. Therefore, the main objective of this transversal study was to evaluate the epidemiologythrough clinical and laboratory evaluation of 225 client-owned dogs with CKD.Materials, Methods & Results: Complete blood count (CBC), urinalysis, and biochemical profile were retrospectivelyselected and evaluated from 225 client-owned dogs with CKD of both sexes, different ages, and breeds from the patientpopulation of the Nephrology and Urology Small Animal Service of the Teaching Hospital of the School of VeterinaryMedicine and Animal Science - São Paulo State University from 2011 to 2017. All dogs were divided in groups according to the International Renal Interest Society (IRIS) CKD grading and statistical analysis was performed according toKruskal-Wallis non-parametric test complemented with Dunn’s multiple comparisons test, and analysis of variance for themodel with a factor complemented with the test of multiple comparisons of Tukey. In this retrospective study, we observedthat most dogs in all groups were elderly (≥ 9 years old). CBC demonstrated lower RBC’s (P < 0.005), hemoglobin (P <0.001), hematocrit (Ht%) [P < 0.001] at the highest stage of the disease. However, urinary specific gravity (USG) did notdemonstrate significant differences between the disease stages, but urinary protein: creatinine ratio (UPC) was statistically different (P < 0.01) between IRIS-CKD stages 1 and 4. Furthermore, serum phosphate concentrations demonstrated...

A transversal study of biochemical profile, urinalysis, UPC, electrolytes and blood pressure in dogs with chronic kidney disease

Background: Chronic kidney disease (CKD) affects both dogs and cats, mainly elderly animals, due to tubulointerstitialinflammation associated with the increase of fibrosis through the excess deposition of extracellular matrix (ECM) whichleads to decrease glomerular filtration. Many different underlying renal diseases can affect the kidneys of dogs such ascongenital or acquired in origin. Therefore, the main objective of this transversal study was to evaluate the epidemiologythrough clinical and laboratory evaluation of 225 client-owned dogs with CKD.Materials, Methods & Results: Complete blood count (CBC), urinalysis, and biochemical profile were retrospectivelyselected and evaluated from 225 client-owned dogs with CKD of both sexes, different ages, and breeds from the patientpopulation of the Nephrology and Urology Small Animal Service of the Teaching Hospital of the School of VeterinaryMedicine and Animal Science - São Paulo State University from 2011 to 2017. All dogs were divided in groups according to the International Renal Interest Society (IRIS) CKD grading and statistical analysis was performed according toKruskal-Wallis non-parametric test complemented with Dunns multiple comparisons test, and analysis of variance for themodel with a factor complemented with the test of multiple comparisons of Tukey. In this retrospective study, we observedthat most dogs in all groups were elderly (≥ 9 years old). CBC demonstrated lower RBCs (P < 0.005), hemoglobin (P <0.001), hematocrit (Ht%) [P < 0.001] at the highest stage of the disease. However, urinary specific gravity (USG) did notdemonstrate significant differences between the disease stages, but urinary protein: creatinine ratio (UPC) was statistically different (P < 0.01) between IRIS-CKD stages 1 and 4. Furthermore, serum phosphate concentrations demonstrated...(AU)

Occurrence of heat waves and the prediction of feed intake of sows raised in a tropical environment

The objective with this study was to estimate the voluntary feed intake (VFI) of sows during the occurrence of heat waves in the Triângulo Mineiro and Alto Paranaíba mesoregion, Minas Gerais, Brazil. The Instituto Nacional de Meteorologia (INMET) database was used to calculate the temperature-humidity index (THI) from 2008 to 2017 for Araxá, Frutal, Ituiutaba, Patos de Minas, Patrocínio, Uberaba, and Uberlândia, and the estimated VFI with and without effective occurrence of heat waves (EOH). An economic estimation of the revenue based on the VFI was performed. The data were analyzed by Friedman's non-parametric test (two-way non parametric analysis), using the significance level of 5%. The sows raised in Patrocínio showed a higher average prediction of VFI (6.611 kg without EOH and 5.743 kg with EOH) than Ituiutaba (6.424 kg without EOH and 3.969 kg with EOH) and Frutal (5.868 kg without EOH and 4.648 kg with EOH). In the classification "without EOH", the microregions of Araxá (6.502 kg), Uberaba (6.452 kg), and Uberlândia (6.478 kg) did not differ from the other cities, but "with EOH", Araxá (4.864 kg) differed from Patrocínio (5.743 kg). The reduction of VFI due to thermal stress represented a revenue reduction of R$ 74.60 per litter. The averages for predicted VFI were lower in the EOH category than in the probable occurrences of heat waves category for all cities. The predominance of heat waves reduces the VFI of sows, particularly in Ituiutaba and Frutal.(AU)

Testicular and seminal evaluation of goats fed hay Cenostigma pyramidale / Avaliação testicular e seminal de caprinos alimentados com feno de Cenostigma pyramidale

This study aimed to assess the possible occurrence of reproductive changes in male goats associated with ingestion of Cenostigma pyramidale hay. Sixteen animals divided into two experimental groups, G1 and G2 (control group) were used. Animals in G1 received 2% of forage, based on live weight (LW), composed of 100% of C. pyramidale, and animals in G2 received 2% of Panicum maximum "Massai' grass hay, based on LW. Both groups received 1% of concentrated feed supplementation based on LW, along with mineralized salt and water ad libitum. The goats were subjected to weighing, testicular biometry, and semen and blood collection every 30 days. After 120 days, the animals were castrated and their testes were collected. Testicular measurements were performed and fragments were collected for histological processing to determine the gonadosomatic index (GSI), diameter of the seminiferous tubules, height of the germinal epithelium (HGE), volumetric proportion and volume of the testicular parenchyma components, total length of the seminiferous tubules, length of the seminiferous tubules per gram of testis, and leydigosomatic and tubulosomatic indexes. The data were evaluated for normality using the Student's t-test. Data with normal distribution were assessed using analysis of variance (ANOVA) and the non-parametric data were evaluated using the Kruskal-Wallis test, both at 5% probability. Statistically significant differences (p<0.05) were observed for GSI (G1=0.48 ±0.08 and G2=0.34 ±0.09) and HGE (G1=52.95 ±2.99 and G2=.47.63 ±2.67) between treatments. Consumption of C. pyramidale hay increased LW and, consequently, testicular weight, contributing to high GSI. In conclusion, ingestion of C. pyramidale has no toxic effect on the testicular, seminal and histological parameters of goat testis. Due to its nutritional characteristics, consumption of this plant improves animal body development. Because C. pyramidale is adapted to semi-arid regions, it can be an alternative source of feed for goats during periods of shortage.(AU)

Para avaliar a possível ocorrência de alterações reprodutivas em caprinos machos associado ao consumo de Cenostigma pyramidale, foram utilizados 16 animais divididos em dois grupos experimentais, G1 e G2 (grupo controle). Animais pertencentes ao G1 receberam 2% de volumoso, com base no peso vivo (PV), constituído de 100% de C. pyramidale e o G2 receberam 2%, com base no PV, de feno de Panicum maximum 'Massai'. Todos os grupos receberam 1%, com base no PV, de suplementação concentrada, além de sal mineralizado e água ad libitum. A cada 30 dias os animais eram submetidos à pesagem, biometria testicular e coletas de sêmen. O sêmen foi avaliado quanto ao volume, turbilhonamento, vigor, motilidade, concentração espermática, defeitos maiores, menores e totais. Após 120 dias os animais foram castrados e os testículos coletados. Foram realizadas as mensurações testiculares e coletados fragmentos para o processamento histológico, para determinação do índice gonadossomático, diâmetro dos túbulos seminíferos, altura do epitélio germinativo, proporção volumétrica e volume dos componentes do parênquima testicular, comprimento total dos túbulos seminíferos, comprimento de túbulo seminífero por grama de testículo, índices leydigossomático e tubulossomático. Os dados foram avaliados quanto à normalidade pelo teste t de Student, os dados com distribuição normal foram analisados por análise de variância com 5% de probabilidade e os não paramétricos, pelo teste de Kruskal-Wallis, a 5% de probabilidade. Houve diferenças significativas (p<0,05) para índice gonadossomático (G1=0,48±0,08 e G2=0,34±0,09) e altura do epitélio germinativo (G1=52,95±2,99 e G2=47,63±2,67) entre os tratamentos. O feno de catingueira promoveu aumento no peso corporal e consequentemente maior peso testicular, o que contribui para elevação do IGS. Concluiu-se que o consumo da C. pyramidale não possui efeito tóxico sobre os parâmetros testiculares, seminais e histológicos do testículo dos caprinos e que a planta, por suas características nutricionais promoveu melhora no desenvolvimento corporal dos animais e por ser uma planta adaptada ao clima do semiárido, se constitui uma fonte alternativa de alimentação para esta categoria animal, durante períodos de escassez de alimento.(AU)

Testicular and seminal evaluation of goats fed hay Cenostigma pyramidale / Avaliação testicular e seminal de caprinos alimentados com feno de Cenostigma pyramidale

This study aimed to assess the possible occurrence of reproductive changes in male goats associated with ingestion of Cenostigma pyramidale hay. Sixteen animals divided into two experimental groups, G1 and G2 (control group) were used. Animals in G1 received 2% of forage, based on live weight (LW), composed of 100% of C. pyramidale, and animals in G2 received 2% of Panicum maximum "Massai' grass hay, based on LW. Both groups received 1% of concentrated feed supplementation based on LW, along with mineralized salt and water ad libitum. The goats were subjected to weighing, testicular biometry, and semen and blood collection every 30 days. After 120 days, the animals were castrated and their testes were collected. Testicular measurements were performed and fragments were collected for histological processing to determine the gonadosomatic index (GSI), diameter of the seminiferous tubules, height of the germinal epithelium (HGE), volumetric proportion and volume of the testicular parenchyma components, total length of the seminiferous tubules, length of the seminiferous tubules per gram of testis, and leydigosomatic and tubulosomatic indexes. The data were evaluated for normality using the Student's t-test. Data with normal distribution were assessed using analysis of variance (ANOVA) and the non-parametric data were evaluated using the Kruskal-Wallis test, both at 5% probability. Statistically significant differences (p<0.05) were observed for GSI (G1=0.48 ±0.08 and G2=0.34 ±0.09) and HGE (G1=52.95 ±2.99 and G2=.47.63 ±2.67) between treatments. Consumption of C. pyramidale hay increased LW and, consequently, testicular weight, contributing to high GSI. In conclusion, ingestion of C. pyramidale has no toxic effect on the testicular, seminal and histological parameters of goat testis. Due to its nutritional characteristics, consumption of this plant improves animal body development. Because C. pyramidale is adapted to semi-arid regions, it can be an alternative source of feed for goats during periods of shortage.(AU)

Para avaliar a possível ocorrência de alterações reprodutivas em caprinos machos associado ao consumo de Cenostigma pyramidale, foram utilizados 16 animais divididos em dois grupos experimentais, G1 e G2 (grupo controle). Animais pertencentes ao G1 receberam 2% de volumoso, com base no peso vivo (PV), constituído de 100% de C. pyramidale e o G2 receberam 2%, com base no PV, de feno de Panicum maximum 'Massai'. Todos os grupos receberam 1%, com base no PV, de suplementação concentrada, além de sal mineralizado e água ad libitum. A cada 30 dias os animais eram submetidos à pesagem, biometria testicular e coletas de sêmen. O sêmen foi avaliado quanto ao volume, turbilhonamento, vigor, motilidade, concentração espermática, defeitos maiores, menores e totais. Após 120 dias os animais foram castrados e os testículos coletados. Foram realizadas as mensurações testiculares e coletados fragmentos para o processamento histológico, para determinação do índice gonadossomático, diâmetro dos túbulos seminíferos, altura do epitélio germinativo, proporção volumétrica e volume dos componentes do parênquima testicular, comprimento total dos túbulos seminíferos, comprimento de túbulo seminífero por grama de testículo, índices leydigossomático e tubulossomático. Os dados foram avaliados quanto à normalidade pelo teste t de Student, os dados com distribuição normal foram analisados por análise de variância com 5% de probabilidade e os não paramétricos, pelo teste de Kruskal-Wallis, a 5% de probabilidade. Houve diferenças significativas (p<0,05) para índice gonadossomático (G1=0,48±0,08 e G2=0,34±0,09) e altura do epitélio germinativo (G1=52,95±2,99 e G2=47,63±2,67) entre os tratamentos. O feno de catingueira promoveu aumento no peso corporal e consequentemente maior peso testicular, o que contribui para elevação do IGS. Concluiu-se que o consumo da C. pyramidale não possui efeito tóxico sobre os parâmetros testiculares, seminais e histológicos do testículo dos caprinos e que a planta, por suas características nutricionais promoveu melhora no desenvolvimento corporal dos animais e por ser uma planta adaptada ao clima do semiárido, se constitui uma fonte alternativa de alimentação para esta categoria animal, durante períodos de escassez de alimento.(AU)

Avaliação cardiorrespiratória e de sedação da associação de dexmedetomidina-butorfanol-midazolam ou detomidina-butorfanol-midazolam em catetos (Pecari tajacu) / Cardiorespiratory and sedation evaluation of the combination of dexmedetomidine-butorphanol-midazolam or detomidine butorphanol-midazolam in collared peccaries (Pecari tajacu)

A presente pesquisa comparou os efeitos cardiorrespiratórios, hemogasométricos e sedativos da associação midazolam (0,41mg/kg) e butorfanol (0,31mg/kg) acrescida de detomidina (157µg/kg) (DTMB) ou dexmedetomidina (36µg/kg) (DXMB) em catetos. Catetos adultos (n=20) foram distribuídos em dois grupos, DTMB ou DXMB. As variáveis (FC, f, PAM, SpO2, EtCO2 e TR) foram avaliadas após aplicação dos fármacos. A sedação foi avaliada por meio de escala analógica visual, relaxamento muscular, postura e resposta auditiva. Foi realizada ANOVA, seguida de teste t pareado (paramétricos) e teste de Mann-Whitney rank-sum test (não paramétricos), com P<0,05. Não foi observada diferença estatística entre os grupos para o período de latência. Observou-se diferença significativa entre grupos para as variáveis f, PAM e SpO2, com maiores valores para DTMB, e EtCO2, com maiores valores para DXMB. Os dois grupos apresentaram redução da FC e da concentração de lactato, bem como aumento da concentração de bicarbonato. A SpO2 permaneceu abaixo de 90%, durante todo o período experimental, nos dois grupos estudados. Os animais dos dois grupos apresentaram sedação profunda e relaxamento muscular máximo. Conclui-se que os dois protocolos testados proporcionaram adequada sedação, podendo ser indicados para contenção química de catetos adultos.(AU)

The study compared the cardiorespiratory, hemogasometric and sedative effects of the combination of midazolam (0.41mg/kg) and butorphanol (0.31mg/kg) plus detomidine (157µg/kg) (DTMB) or dexmedetomidine (36µg/kg) (DXMB) in collared peccaries. Collared peccaries (n= 20) were divided into two groups, either DTMB or DXMB. The variables (FC, f, PAM, SpO2, EtCO2 and TR) were evaluated after application of the drugs. Sedation was assessed by visual analogue scale, muscle relaxation, posture and auditory response. ANOVA followed by paired t-test (parametric) and Mann Whitney Rank Sum Test (non-parametric) with P< 0.05 were performed. No statistical difference was observed for the latency period. A significant increase was observed between groups for the variables f, PAM and SpO2 with higher values for DTMB and EtCO2 with higher values for DXMB. The two groups presented a reduction in HR and lactate concentration, and an increase in bicarbonate concentration. SpO2 remained below 90% throughout the experiment in both groups. The animals of the two groups presented deep sedation and maximum muscle relaxation. It is concluded that the two protocols tested provided adequate sedation and could be indicated for chemical containment of collared peccaries.(AU)

Avaliação cardiorrespiratória e de sedação da associação de dexmedetomidina-butorfanol-midazolam ou detomidina-butorfanol-midazolam em catetos (Pecari tajacu) / Cardiorespiratory and sedation evaluation of the combination of dexmedetomidine-butorphanol-midazolam or detomidine butorphanol-midazolam in collared peccaries (Pecari tajacu)

A presente pesquisa comparou os efeitos cardiorrespiratórios, hemogasométricos e sedativos da associação midazolam (0,41mg/kg) e butorfanol (0,31mg/kg) acrescida de detomidina (157µg/kg) (DTMB) ou dexmedetomidina (36µg/kg) (DXMB) em catetos. Catetos adultos (n=20) foram distribuídos em dois grupos, DTMB ou DXMB. As variáveis (FC, f, PAM, SpO2, EtCO2 e TR) foram avaliadas após aplicação dos fármacos. A sedação foi avaliada por meio de escala analógica visual, relaxamento muscular, postura e resposta auditiva. Foi realizada ANOVA, seguida de teste t pareado (paramétricos) e teste de Mann-Whitney rank-sum test (não paramétricos), com P<0,05. Não foi observada diferença estatística entre os grupos para o período de latência. Observou-se diferença significativa entre grupos para as variáveis f, PAM e SpO2, com maiores valores para DTMB, e EtCO2, com maiores valores para DXMB. Os dois grupos apresentaram redução da FC e da concentração de lactato, bem como aumento da concentração de bicarbonato. A SpO2 permaneceu abaixo de 90%, durante todo o período experimental, nos dois grupos estudados. Os animais dos dois grupos apresentaram sedação profunda e relaxamento muscular máximo. Conclui-se que os dois protocolos testados proporcionaram adequada sedação, podendo ser indicados para contenção química de catetos adultos.(AU)

The study compared the cardiorespiratory, hemogasometric and sedative effects of the combination of midazolam (0.41mg/kg) and butorphanol (0.31mg/kg) plus detomidine (157µg/kg) (DTMB) or dexmedetomidine (36µg/kg) (DXMB) in collared peccaries. Collared peccaries (n= 20) were divided into two groups, either DTMB or DXMB. The variables (FC, f, PAM, SpO2, EtCO2 and TR) were evaluated after application of the drugs. Sedation was assessed by visual analogue scale, muscle relaxation, posture and auditory response. ANOVA followed by paired t-test (parametric) and Mann Whitney Rank Sum Test (non-parametric) with P< 0.05 were performed. No statistical difference was observed for the latency period. A significant increase was observed between groups for the variables f, PAM and SpO2 with higher values for DTMB and EtCO2 with higher values for DXMB. The two groups presented a reduction in HR and lactate concentration, and an increase in bicarbonate concentration. SpO2 remained below 90% throughout the experiment in both groups. The animals of the two groups presented deep sedation and maximum muscle relaxation. It is concluded that the two protocols tested provided adequate sedation and could be indicated for chemical containment of collared peccaries.(AU)

Comparison of non-parametric indexes to select soybean genotypes obtained by recurrent selection / Comparação de índices não paramétricos na seleção de genótipos superiores de soja obtidos por seleção recorrente

In the final steps of a breeding program, it is necessary to evaluate several traits, which makes it difficult to select the superior genotypes. This study aimed to compare nonlinear indexes in the selection of superior soybean inbred lines obtained by recurrent selection. The experiment was carried out in Londrina, Paraná State, Brazil. During the 2010/11 and 2011/12 seasons, 67 soybean inbred lines and two commercial controls (BMX Potência RR and NK 7059 RR) were evaluated. The experimental design was a randomized complete block with three replicates. The evaluated traits were: days to maturity, agronomic value, lodging, first pod insertion height, and grain yield. A combined analysis of variance for seasons was performed and the sum of ranks index, ideotype distance index, and cultivars selection index were applied. All traits were significant for the genotypic source of variation and showed complex genotype × environment interactions. The selection indexes were consistent with each other. The ideotype and cultivar selection index showed higher concordance on indication of 15 superior inbred lines with a coefficient of coincidence of 80%. The inbred lines SR-03, SR-12, SR-22, SR-41, SR-49, SR-55, SR-61, and SR-62 were indicated for all indexes and are therefore considered superior to other inbred lines.(AU)

Nas etapas finais de um programa de melhoramento é necessário a avaliação de diversos caracteres, o que gera dificuldade na escolha dos melhores genótipos. O presente trabalho teve como objetivo comparar índices não lineares na seleção de linhagens superiores de soja, obtidas por seleção recorrente. Os experimentos foram conduzidos em Londrina, Paraná, Brasil. Durante as safras 2010/11 e 2011/12 foram avaliados sessenta e sete linhagens de soja e duas testemunhas comerciais ((BMX Potência RR e NK 7059 RR). O delineamento experimental adotado foi em blocos completos ao acaso, com três repetições. As características avaliadas foram: dias para maturação, valor agronômico, acamamento, inserção da primeira vagem e produtividade de grãos. Realizou-se a análise de variância conjunta para anos agrícolas e empregaram-se os índices de soma de ranks, de distância ao ideótipo e de seleção de cultivares. Todas características para a fonte de variação genótipo foram significativas e apresentaram predomínio da interação complexa na interação genótipo x ambiente. Os índices de seleção se mostraram concordantes entre si. O índice de distância ao ideótipo e o índice de seleção de cultivar apresentaram maior concordância na indicação das 15 melhores linhagens com coeficiente de coincidência de 80%. As linhagens SR-03, SR-12, SR-22, SR-41, SR-49, SR-55, SR-61 e SR-62 foram indicadas em todos os índices e, portanto, consideradas superiores às demais.(AU)