Intake and apparent nutrient digestibility, ruminal parameters, and nitrogen balance of lambs fed with corn and forage-free diets with sodium bicarbonate / Consumo e digestibilidade aparente dos nutrientes, parâmetros ruminais e balanço de N de cordeiros confinados recebendo dietas com milho e sem forragem com bicarbonato de sódio

This study evaluated forage-free diets supplemented with sodium bicarbonate (SB) based on ground flint corn on intake, apparent nutrient digestibility, ruminal parameters, and N balance. Four rumen-cannulatedDorper × Santa Inês lambs, with a body weight of 43.4 ± 0.85 kg (mean ± standard deviation) were used. The experimental design was a 4×4 Latin square. Each experimental period lasted 21 days. Experimental diets were: positive control, a diet without SB containing 400 g/kg DM of soybean hulls (CONT), and three diets based on ground flint corn, containing 10 (10SB), 20 (20SB) or 30 (30SB) g/kg DM of SB. Intake of neutral and acid detergent fiber was greater for CONT (P < 0.01) when compared to SB. Intake and apparent nutrient digestibility of dry and organic matter, ether extract, and crude protein did not differ. Intake (P = 0.01) and digestibility (P < 0.01) of non-fiber carbohydrates were greater for SB diets and neutral detergent fiber digestibility was greater (P = 0.03) for CONT. The total digestible nutrients and metabolizable energy were greater for SB (P = 0.04). Rumen concentrations of acetate (P < 0.01), isobutyrate (P < 0.01), butyrate (P < 0.01), isovalerate (P < 0.01), and total short-chain fatty acids (P < 0.01) were greater for CONT. Ruminal pH showed a quadratic (P = 0.05) response to SB levels, with greater values for 20SB. Sodium bicarbonate was efficient in increasing ruminal pH at the level of 20 g/kg DM without interfering with the intake and apparent nutrient digestibility characteristics.

O presente estudo avaliou a suplementação de bicarbonato de sódio (BS) em dietas sem forragem à base de milho moído sobre o consumo e a digestibilidade aparente dos nutrientes, os parâmetros ruminais e o balanço de N. Foram utilizados quatro cordeiros Dorper × Santa Inês, com um peso corporal inicial de 43,4 ± 0,85 kg (média ± desvio padrão). O delineamento experimental foi quadrado latino 4×4. Cada período experimental durou 21 dias. As dietas experimentais foram: controle positivo, sem BS, contendo 400 g/kg de MS de casca de soja (CONT) e três dietas à base de milho moído, sem casca de soja, contendo 10 (10BS), 20 (20BS) ou 30 (30BS) g/kg de MS de BS. O consumo de fibra em detergente neutro e ácido foi maior (P < 0,01)para o CONT quando comparado com BS. O consumo e a digestibilidade aparente da matéria seca, matéria orgânica, extrato etéreo e proteína bruta não diferiram entre os tratamentos. O consumo (P = 0,01) e digestibilidade (P < 0,01) dos carboidratos não fibrosos foram superiores para as dietas contendo BS e a digestibilidade da fibra em detergente neutro foi maior para o CONT. Os teores de nutrientes digestivos totais e energia metabolizável foram superiores para as dietas contendo BS (P = 0,04). A concentração ruminal de acetato (P < 0,01), isobutirato (P < 0,01), butirato (P < 0,01), isovalerato (P < 0,01) e ácidos graxos totais de cadeia curta (P < 0,01) foram mais elevadas para o CONT. O pHruminal apresentou resposta quadrática (P = 0,05) em relação aos níveis de BS, sendo observado maior pH para os animais alimentados com a dieta 20BS. O BS foi eficiente em aumentar o pHruminal no teor de 20 g/kg MS sem interferir no consumo e na digestibilidade aparente dos nutrientes. Portanto, 20 g/kg de MS é a dose indicada de BS em dietas sem forragem para terminação de cordeiros em confinamento.

Chitosan combined with technical cashew nut shell liquid improves in vitro ruminal parameters and gas production kinetics

The objective was to evaluate the inclusion of chitosan (CHI) and technical cashew nut shell liquid (CNSLt) as natural feed additives in cattle diets on nutrient digestibility, ruminal fermentation, and in vitro gas production kinetics. We conducted a completely randomized design with 5×4 factorial arrangement, with 20, 35, 50, 65, and 100% Tifton 85 hay and four additives, monensin (200 mg/kg DM), CNSLt (500 mg/kg DM), CHI (500 mg/kg DM), and CNSLt+CHI (500 mg/kg DM/each). Dry matter (DM) and organic matter (OM) digestibility showed a linear reduction according to forage levels. The highest DM digestibility was observed with CHI on cattle diets. Inclusion of CHI increased DM digestibility. The highest in vitro organic matter and crude protein (CP) digestibilities were observed for CNSLt+CHI. The in vitro dry matter digestibility increased linearly with concentrate in the diet. There was interaction of forage:concentrate ratio and the additives for neutral detergent fiber, acid detergent fiber and hemicellulose digestibility. Chitosan, CNSLt, and CNSLt+CHI promoted the lowest acetate:propionate ratio compared with monensin. Total gas production showed interaction of the forage:concentrate ratio and additives. Lag time was lowest with CNSLt+CHI. Chitosan and CNSLt can be considered alternative fermentation modulators to ionophores by improving nutrient digestibility and increasing ruminal propionate concentrations.(AU)

Cellular components and TGF-beta1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse] / [Componentes celulares e TGF-beta1 do plasma rico em plaquetas obtido por sistema fechado em equinos]

Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-ß1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1.6-fold higher platelet and approximately 2.0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.

O plasma rico em plaquetas (PRP) tem sido proposto como um agente para acelerar o processo de cicatrização e estimular a capacidade regenerativa dos tecidos, devido à sua abundância de fatores de crescimento. Uma grande variedade de kits e de protocolos está disponível para obtenção de PRP, utilizando-se diferentes sistemas de separação de células. No entanto, a falta de padronização pode levar a resultados inconsistentes. O objetivo deste estudo foi caracterizar a composição celular, os parâmetros plaquetários pelo citômetro de fluxo ADVIA 120 e a concentração de TGF-ß1 do PRP obtido em sistema fechado, por centrifugação simples. Seis cavalos clinicamente saudáveis ​​foram usados ​​neste estudo. O protocolo no sistema fechado resultou em concentrações de plaquetas aproximadamente 1,6 vez maior e concentrações de leucócitos aproximadamente 2,0 vezes menores em comparação com os valores do sangue total. O sistema avaliado foi eficiente na concentração de plaquetas e na recuperação de um pequeno número de leucócitos, utilizando um protocolo de centrifugação única em baixa velocidade.

Plasticizer types and whey protein coatings on internal quality and shelf life of eggs stored for 42 days

ABSTRACT: Effects of plasticizer types and whey protein concentrate (WPC) as coatings were evaluated on internal quality and shelf life of eggs stored for 42 days. Eggs were coated with WPC at 8 % solution combined with the plasticizers glycerol (GLY), sorbitol (SOR), and propylene glycol (PRO). The eggs were stored at 20 °C. Weight loss, Haugh Unit (HU), albumen and yolk pH, and yolk index (YI) were evaluated weekly from day 0 to 42 days. After, electron microscopy of the eggshell structure was performed. The data was submitted to the analysis of variance and the effects of treatment, storage time, and the interaction between these factors were evaluated. There was significant interaction between factors (treatment and periods) for weight loss (p 0.0001), HU (p 0.0001), albumen (p 0.0001), and yolk pH (p 0.0001), and YI (p 0.0001). After 42 days, uncoated eggs showed greater weight loss (5.4 %), compared to WPC+GLY, (3.8 %), WPC+SOR (3.3 %) and WPC+PRO (3.9 %). Similar results were verified for HU at 42 days of storage. Uncoated eggs showed HU of 58.46 (B), while coated eggs showed higher values: WPC+GLY 66.58 (A), WPC+SOR 68.79 (A), and WPC + PRO 71.53 (A). The plasticizers GLY, SOR and PRO, associated with WPC, demonstrated effectiveness in maintaining the quality of eggs throughout the 42 days of storage. However, WPC+SOR showed superiority in preserving quality integrity of eggs. This result can be related to the chemical structure of SOR, making the combination more efficient for storage.

Effect of high hydrostatic pressure on the antioxidant capacity and peptic hydrolysis of whey proteins / Efeito da alta pressão hidrostática na hidrólise péptica das proteínas do soro de leite e na capacidade antioxidante

The effect of high hydrostatic pressure (HHP) application on whey protein concentrate was evaluated both before (pre-treatment - PT) and during (hydrolysis assisted - HA) hydrolysis processes. A factorial design 22 with 3 central points was used with pressure (100, 250, 400 MPa) and time (5, 20 and 35 minutes) as independent variables. The hydrolysis was evaluated and monitored by soluble protein, aromatic amino acid contents and RP-HPLC. ABTS and ORAC tests were used to evaluate the in vitro antioxidant capacity. The reduction of soluble protein content was approximately 20% for conventional hydrolysis and for all PT treatments up to 4 h of reaction, while HHP assisted hydrolysis at 100 MPa showed a 35% protein reduction after 35 minutes of reaction. In addition, pressurization favored peptic hydrolysis of -lactoglobulin by up to 98% and also improved the in vitro antioxidant capacity of the hydrolysates, which increased from 34.25 to 60.89 moles TE g-1 of protein in the best treatment. The results suggest that the use of HHP assisted hydrolysis favored the peptic hydrolysis, with a reduction in hydrolysis time and increased antioxidant activity.(AU)

Neste estudo, o efeito da aplicação de alta pressão hidrostática (HHP) sobre o concentrado proteico de soro de leite foi avaliado antes (pré-tratamento - PT) e durante os processos de hidrólise (assistida por hidrólise - HA). Utilizou-se o delineamento fatorial 22 com três pontos centrais, onde as variáveis independentes foram pressão (100, 250, 400 MPa) e tempo (5, 20 e 35 minutos). A hidrólise foi avaliada pelo conteúdo de proteínas solúveis e aminoácidos aromáticos, além do perfil peptídico por RP-HPLC. As análises de ABTS e ORAC foram utilizadas para avaliar a capacidade antioxidante in vitro. A redução do teor de proteína solúvel foi de aproximadamente 20% para a hidrólise convencional e para todos os pontos de PT até 4h de reação, enquanto a hidrólise assistida por HHP a 100 MPa mostrou uma redução de 35% de proteína em 35 minutos de reação. Além disso, a pressurização favoreceu a hidrólise péptica da -lactoglobulina em até 98% e também melhorou a capacidade antioxidante in vitro dos hidrolisados, que aumentaram de 34,25 para 60,89 moles de TE g-1 de proteína no melhor tratamento. Os resultados sugerem que o uso da hidrólise assistida por HHP favoreceu a hidrólise péptica, com redução no tempo de hidrólise e aumento da atividade antioxidante.(AU)

Meat quality of lambs fed phytogenic additives / Qualidade da carne de cordeiros alimentados com aditivos fitogênicos

The study was developed in the Sheep Production Laboratory of the Department of Animal Science, College of Agricultural and Veterinary Sciences, São Paulo State University, Jaboticabal, SP, Brazil. Thirtytwo non-castrated Ile de France lambs weighing 20.0 ± 0.2 kg received 4 diets with a roughage: concentrate ratio of 40: 60. The experimental treatments consisted of D1: corn silage + concentrate; D2: corn silage + concentrate + 30 mg rosemary extract kg body weight1; D3: corn silage + concentrate + 30 mg green tea extract kg body weight-1; and D4: corn silage + concentrate + 30 mg oregano extract kg body weight-1, up to 35.0 ± 0.2 kg body weight. The objectives were to evaluate rib eye area, cholesterol content, lipid oxidation, qualitative parameters, as well as perform sensory analysis, centesimal composition, and fatty acid profile of the meat. The experimental design was completely randomized with four treatments and eight replicates. The treatments were compared by Tukey's test through the SAS software (Statistical Analysis System Institute [SAS], 2003). Inclusion of phytogenic additives into the diets did not affect the qualitative parameters cholesterol content, lipid oxidation, and centesimal composition (except for protein content, which was lower in D2, 22.57%, compared to D1, 25.62%), as well as meat fatty acid profile (except for C18: 1n7 cis-vaccenic). The use of phytogenic additives provided suitable qualitative parameters. Moreover, despite the well-known positive effect of antioxidants in the diet, phytogenic additive inclusion was ineffective in reducing lipid oxidation in lamb meat. However, further studies with different inclusion percentages should be carried out to find out how to increase shelf life without harming lamb qualitative parameters, especially regarding the sensory analysis.(AU)

O trabalho foi realizado no Laboratório de Produção Ovina, pertencente ao Departamento de Zootecnia da FCAV, Unesp, Câmpus de Jaboticabal, SP. Trinta e dois cordeiros Ile de France, machos não castrados, com 20,0 ± 0,2 kg receberam 4 dietas, com relação volumoso:concentrado 40:60, sendo os tratamentos constituídos por D1: silagem de milho + concentrado, D2: silagem de milho + concentrado + 30 mg de extrato de alecrim kg peso corporal-1, D3: silagem de milho + concentrado + 30 mg de extrato de chá verde kg de peso corporal-1 e D4: silagem de milho + concentrado + 30 mg de extrato de orégano kg de peso corporal-1, até atingirem 35,0 ± 0,2 kg de peso corporal. Os objetivos foram avaliar a área de olho de lombo, o teor de colesterol, a oxidação lipídica, os parâmetros qualitativos, bem como a análise sensorial, a composição centesimal e o perfil de ácidos graxos da carne. O delineamento experimental foi o inteiramente casualizado, com quatro tratamentos e oito repetições, sendo as comparações entre as médias dos tratamentos feitas pelo teste Tukey a 5 %, e as análises de variância conforme procedimentos do SAS Software (Statistical Analysis System Institute [SAS], 2003). A inclusão de aditivos fitogênicos na dieta de cordeiros não afetou os parâmetros qualitativos da carne, o teor de colesterol, a oxidação lipídica e a composição centesimal (com exceção do teor de proteína que foi menor na D2, 22,57%, em relação à D1, de 25,62%) e o perfil de ácidos graxos (com exceção do C18:1n7 cis-vacênico). O uso dos aditivos fitogênicos na dieta dos cordeiros propiciou adequadas características qualitativas da carne ovina, e apesar do conhecido efeito positivo do uso de antioxidantes na dieta de cordeiros, a inclusão dos aditivos fitogênicos não foi eficaz em reduzir a oxidação lipídica da carne, entretanto, novos estudos com diferentes porcentagens de inclusão devem ser conduzidos para averiguar como aumentar a vida de prateleira da carne sem prejudicar as características qualitativas da carne, principalmente em relação à análise sensorial.(AU)

Soybean meal from damaged grains replacing standard soybean meal in diets of feedlot lambs

Two studies were carried out to evaluate the effects of levels of soybean meal produced from damaged soybean grains replacing normal soybean meal on in vitro and in situ digestibility of ruminant diets as well as on intake, digestibility, and animal performance of growing/finishing lambs. In trial 1, we evaluated the in vitro digestibility of each soybean meals (normal and damaged), as well as diets containing levels of the damaged replacing the normal (0, 333, 667 and 1,000 g.kg−1) soybean meal on kinetic parameters of in vitro cumulative gas production, in vitro dry matter and crude protein digestibility, in situ rumen-degradable protein, rumen-undegradable protein, and in vitro intestinal digestibility of rumen-undegradable protein. In trial 2, we used 48 growing/finishing lambs to evaluate the effects of damaged soybean meal levels (0, 333, 667, and 1,000 g.kg−1) replacing normal soybean meal in feedlot diets (20:80 roughage:concentrate ratio) on intake and digestibility of nutrients and on animal performance. The damaged soybean meal presented lower values for total gas production and in vitro dry matter digestibility than normal soybean meal. Higher rumen-undegradable protein was estimated for damaged soybean meal than for the normal and consequently lower rumen-degradable protein for damaged compared to normal. Because of the lower rumen-degradable protein, damaged soybean meal promoted lower in vitro ammonium nitrogen (NH3 -N) concentrations than the normal in feedlot diets. In the in vivo trial, there were no effects of damaged soybean meal levels in the diets on intake and digestibility of nutrients (dry matter, organic matter, crude protein, and fiber) as well as on total weight gain, average daily gain, carcass yield, or feeding efficiency. Thus, damaged soybean meal can fully replace the normal one in lamb feedlot diets (in up to of 1,000 g.kg−1 of the normal soybean meal) without causing adverse effects on intake and digestibility of nutrients and on animal performance.

Nutritional and quality changes in piglet concentrate affected by the mix / [Mudanças nutricionais e de qualidade no concentrado de leitão afetadas pela mistura]

The aim of this study was to evaluate the reasons of quality deviation of a concentrate from a predefined standard. Five treatments were established: T1 - Control, standard concentrate formulation (SCF); T2 - PXMore5, SCF with more 5% vitamin-mineral premix (VMP); T3 - PXLess5, SCF with less 5% VMP. All three treatments used a 400kg batches in an INTECNIAL mixer; T4 - FeedMixer, SCF using a 4,000kg batch in an IMOTO mixer; T5 - PremixMixer, SCF using a 1,200kg batch in an MUYANG mixer. For each treatment, bags of 20 kg were stored in three storage places for four months. Water activity of concentrate was affected by temperature and air relative humidity in different storage places. Regarding the kind of mixer, the greatest variation in concentration of crude protein, mineral residue, copper, zinc, and selenium was observed in the PremixMixer. Adjustments are imperative in the handling and use procedures of this kind of mixer to meet the quality requirements required in the concentrate production. Analyzing the effect of the mineral-vitamin premix level, no difference could be defined with the evaluated parameters.(AU)

O objetivo deste estudo foi avaliar as razões do desvio de qualidade de um concentrado de um padrão predefinido. Foram estabelecidos cinco tratamentos: T1 - controle, concentrado com formulação padrão (CFP); T2 - PXMais5, CFP com 5% a mais de vitaminas e minerais da pré-mistura (PVM); T3 - PXMenos5, CFP com 5% a menos de PVM. Todos esses três tratamentos utilizaram lotes de 400kg em um misturador INTECNIAL; T4 - FeedMixer, CFP usando um lote de 4.000kg em um misturador IMOTO; T5 - PremixMixer, CFP usando um lote de 1.200kg em um misturador MUYANG. Para cada tratamento, sacos de 20kg foram armazenados em três ambientes distintos por quatro meses. A atividade de água do concentrado foi afetada pela temperatura e umidade relativa do ar em diferentes locais de armazenamento. Em relação ao tipo de misturador, a maior variação na concentração de proteína bruta, resíduo mineral, cobre, zinco e selênio foi devido ao PremixMixer. Ajustes são imperativos nos procedimentos de manuseio e uso desse tipo de misturador para atender aos requisitos de qualidade exigidos na produção de concentrado. Ao se analisar o efeito do nível da pré-mistura de vitaminas e minerais, nenhuma diferença pôde ser definida com os parâmetros avaliados.(AU)

Yellow grease in sheep diets: intake and digestibility / [Óleo residual de fritura em dietas para ovinos: consumo e digestibilidade]

This study aimed to assess the effects of yellow grease supplementation on the intake, digestibility, and nitrogen balance in sheep. Twenty Santa Inês lambs with a mean age of 95 ± 10 d and body weight of 19.29 ± 3.17kg were evaluated in a completely randomized design. The diets were supplemented with oil at concentrations of 0, 20, 40, 60, and 80 gkg-1 of dry matter (DM) of the concentrate. The diets were based on roughage and concentrate (50:50). The experimental period lasted 19 d and included 14 adaptation days and five collection days for the total supplied diet, orts, feces, and urine. Supplementation with yellow grease had no significant effect on the intake of DM, crude protein (CP), neutral detergent fiber (NDF), or non-fiber carbohydrates (NFC). However, the ether extract (EE) intake increased linearly with supplementation of yellow grease. Moreover, no effect was observed for DM, CP, NDF, and NFC digestibility and nitrogen balance. EE digestibility increased linearly with the yellow grease dietary supplementation. Thus, sheep dietary supplementation with yellow grease may be used at a level of up to 80 gkg-1 of DM of concentrate without impairing nutrient intake and digestibility.(AU)

Objetivou-se, com o estudo, avaliar os efeitos do óleo residual de fritura, em dietas para ovinos, sob o consumo, a digestibilidade e o balanço de nitrogênio. Foram utilizados 20 cordeiros Santa Inês, com idade de 95 ± 10 dias e peso corporal de 19,29 ± 3,17kg, em delineamento inteiramente ao acaso. As dietas continham óleo de fritura nas concentrações de 0; 20; 40; 60 e 80gkg-1 da matéria seca (MS) do concentrado. As dietas tinham relação volumoso:concentrado de 50:50. O período experimental foi de 19 dias, incluindo 14 dias em adaptação e cinco dias de coleta do fornecido, das sobras, das fezes e da urina. A suplementação com óleo de fritura não alterou o consumo de MS, proteína bruta (PB), matéria orgânica (MO), fibra em detergente neutro (FDN) e carboidratos não fibrosos (CNF). Entretanto, o consumo de extrato etéreo (EE) aumentou com a inclusão do óleo. Não foi observado efeito na digestibilidade da MS, da PB, da FDN, dos CNF e no balanço de nitrogênio. A digestibilidade do EE aumentou com a inclusão do óleo. Assim, a inclusão de óleo de fritura em dietas para ovinos pode ser utilizada em até 80gkg-1 da MS do concentrado, sem limitar ingestão e digestibilidade dos nutrientes.(AU)

Plasticizer types and whey protein coatings on internal quality and shelf life of eggs stored for 42 days

Effects of plasticizer types and whey protein concentrate (WPC) as coatings were evaluated on internal quality and shelf life of eggs stored for 42 days. Eggs were coated with WPC at 8 % solution combined with the plasticizers glycerol (GLY), sorbitol (SOR), and propylene glycol (PRO). The eggs were stored at 20 °C. Weight loss, Haugh Unit (HU), albumen and yolk pH, and yolk index (YI) were evaluated weekly from day 0 to 42 days. After, electron microscopy of the eggshell structure was performed. The data was submitted to the analysis of variance and the effects of treatment, storage time, and the interaction between these factors were evaluated. There was significant interaction between factors (treatment and periods) for weight loss (p < 0.0001), HU (p < 0.0001), albumen (p < 0.0001), and yolk pH (p < 0.0001), and YI (p < 0.0001). After 42 days, uncoated eggs showed greater weight loss (5.4 %), compared to WPC+GLY, (3.8 %), WPC+SOR (3.3 %) and WPC+PRO (3.9 %). Similar results were verified for HU at 42 days of storage. Uncoated eggs showed HU of 58.46 (B), while coated eggs showed higher values: WPC+GLY – 66.58 (A), WPC+SOR – 68.79 (A), and WPC + PRO – 71.53 (A). The plasticizers GLY, SOR and PRO, associated with WPC, demonstrated effectiveness in maintaining the quality of eggs throughout the 42 days of storage. However, WPC+SOR showed superiority in preserving quality integrity of eggs. This result can be related to the chemical structure of SOR, making the combination more efficient for storage.

Plasticizer types and whey protein coatings on internal quality and shelf life of eggs stored for 42 days

Effects of plasticizer types and whey protein concentrate (WPC) as coatings were evaluated on internal quality and shelf life of eggs stored for 42 days. Eggs were coated with WPC at 8 % solution combined with the plasticizers glycerol (GLY), sorbitol (SOR), and propylene glycol (PRO). The eggs were stored at 20 °C. Weight loss, Haugh Unit (HU), albumen and yolk pH, and yolk index (YI) were evaluated weekly from day 0 to 42 days. After, electron microscopy of the eggshell structure was performed. The data was submitted to the analysis of variance and the effects of treatment, storage time, and the interaction between these factors were evaluated. There was significant interaction between factors (treatment and periods) for weight loss (p < 0.0001), HU (p < 0.0001), albumen (p < 0.0001), and yolk pH (p < 0.0001), and YI (p < 0.0001). After 42 days, uncoated eggs showed greater weight loss (5.4 %), compared to WPC+GLY, (3.8 %), WPC+SOR (3.3 %) and WPC+PRO (3.9 %). Similar results were verified for HU at 42 days of storage. Uncoated eggs showed HU of 58.46 (B), while coated eggs showed higher values: WPC+GLY 66.58 (A), WPC+SOR 68.79 (A), and WPC + PRO 71.53 (A). The plasticizers GLY, SOR and PRO, associated with WPC, demonstrated effectiveness in maintaining the quality of eggs throughout the 42 days of storage. However, WPC+SOR showed superiority in preserving quality integrity of eggs. This result can be related to the chemical structure of SOR, making the combination more efficient for storage.(AU)

Accuracy and physical characteristics of total mixed rations and feeding sorting behavior in dairy herds of Castro, Parana

The objective of this study was to assess the accuracy of the content of total mixed rations (TMR) offered to high-producing cows from 20 dairy herds of Castro, Paraná State, Brazil. The average milk yield during the sample collection period was 38.0±6.8 kg/day, with 3.47±0.25% milk fat and 3.05±0.18% milk protein. The particle size distribution of samples of fresh diet and leftovers was evaluated using the Penn State Particle Separator (PSPS) and chemical analysis was also conducted. The TMR homogeneity was estimated by the coefficient of variation (CV) of particles retained on the two intermediary sieves (1.18 mm), in which a CV higher than 5% indicated a poorly mixed TMR. The TMR offered exceeded the formulated TMR only for NDF (+7.3%) and was lower than the formulated TMR for CP (−6.5%), ADF (−10.1%), and ash (−18.6%) contents. These differences are due to daily variations in feed quality, mixing equipment, and ingredient mixing order, mostly for forage. Differences between leftovers and TMR offered were substantial: −9.4% for CP, +25.1% for NDF, +31.6% for ADF, and +13.1% for ash, suggesting a feed sorting effect against long forage particles and in favor of small concentrate particles. The proportion of the offered TMR retained in the top PSPS sieve showed positive correlations with NDF (r = 0.58) and ADF (r = 0.54) contents of the refusals, which indicates that cows sort against long particles. Positive correlations were also found between the CV in the second sieve and NDF (r = 0.56) and ADF (r = 0.47) contents of refusals, suggesting that poorly mixed diets are more easily sorted by cows. Therefore, evaluating the chemical and physical properties of TMR, especially for forage, is extremely important for providing a consistent diet.

Partial substitution of fishmeal with soybean protein-based diets for dourado Salminus brasiliensis

Cost and environmental restrictions limit the use of fishmeal (FM) in aquafeeds and plant protein (PP) sources. Soybean by-products are important alternative; however, their antinutritional factors may reduce fish growth. This study evaluates the effect of FM substitution for PP (soybean meal and soy protein concentrate) in diets of dourado (Salminus brasiliensis), a promising species for fish farming and empirically considered highly tolerant to PP-based diets. A control diet was formulated to contain 20 % of fishmeal and 61 % of plant protein (SBM) and other five diets with decreasing FM graded levels (16 %, 12 %, 8 %, 4 %, 0 %), which were replaced by soybean protein concentrate (SPC), comprising six treatments and four replicates. Fifteen dourados (6.73 ± 0.2 g) were stocked into 200 L tanks in a recirculating system, fed with experimental diets three times a day to apparent satiation for 90 days. Growth performance, food conversion ratio, protein efficiency ratio and protein retention efficiency of fish were impaired when FM substitution exceeded 13.05 % (the broken line analysis method). Histological analysis of intestinal mucosa showed a decrease in villus height when fishmeal was totally replaced by PP, which probably impaired digestive functions of fish and reflected in a severe loss on growth performance.

Partial substitution of fishmeal with soybean protein-based diets for dourado Salminus brasiliensis

Cost and environmental restrictions limit the use of fishmeal (FM) in aquafeeds and plant protein (PP) sources. Soybean by-products are important alternative; however, their antinutritional factors may reduce fish growth. This study evaluates the effect of FM substitution for PP (soybean meal and soy protein concentrate) in diets of dourado (Salminus brasiliensis), a promising species for fish farming and empirically considered highly tolerant to PP-based diets. A control diet was formulated to contain 20 % of fishmeal and 61 % of plant protein (SBM) and other five diets with decreasing FM graded levels (16 %, 12 %, 8 %, 4 %, 0 %), which were replaced by soybean protein concentrate (SPC), comprising six treatments and four replicates. Fifteen dourados (6.73 ± 0.2 g) were stocked into 200 L tanks in a recirculating system, fed with experimental diets three times a day to apparent satiation for 90 days. Growth performance, food conversion ratio, protein efficiency ratio and protein retention efficiency of fish were impaired when FM substitution exceeded 13.05 % (the broken line analysis method). Histological analysis of intestinal mucosa showed a decrease in villus height when fishmeal was totally replaced by PP, which probably impaired digestive functions of fish and reflected in a severe loss on growth performance.(AU)

Extruded feed in different volume-concentrate ratios: in-vitro fermentation kinetics, consumption, and evaluation of lambs’ metabolic profile / Ração extrusada em diferentes relações de volumoso e concentrado: cinética de fermentação in vitro e avaliação do perfil metabólico de borregas

This study aimed both to evaluate the metabolic profile of lambs fed with extruded forage and concentrate containing different roughageconcentrate ratios (R:C) and to evaluate in-vitro ruminal fermentation kinetics of these rations. An experiment was carried out with 20 weanling, female crossbred lambs (½Dorper x ½Santa Inês) aged 120 ± 8 days. The treatments were four R:C ratios of extruded forage (Foragge®) and extruded concentrate (Beef Total®) – 30%F:70%C, 40%F:60%C, 50%F:50%C, and 60%F:40%C – distributed randomly with five replicates. The analysis targeted energy metabolites (VLDL, triglycerides, and cholesterol), hepatic metabolites (alkaline phosphatase, gamma glutamyltransferase [GGT], and aspartate aminotransferase [AST]), and protein metabolites (total protein, uric acid, urea, creatinine, albumin, and globulin). In-vitro gas production was quantified by inoculating the extruded rations in bovine ruminal fluid with the following forage:concentrate proportions evaluated: 30%F:70%C, 40%F:60%C, 50%F:50%C, 60%F:40%C and 70%F:30%C. Fermentation kinetic values were compared using parallelism test and curve identity at the error probability level of 5%. A regression study was used to compare the means of the metabolites at the error probability level of 5%. Average dry matter intake data are provided descriptively. The extruded ration increased the hepatic metabolites (GGT and AST) without compromising the liver and kept the energy metabolites stable. Similarly, the serum concentration of protein metabolites remained at normal levels recommended to the ovine species. In-vitro fermentation pattern displayed high gas production associated with high ration digestibility. The extruded ration provides protein and energy supply to meet the animals’ nutritional requirements without compromising their health.

Objetivou-se avaliar (i) o perfil metabólico de borregas alimentadas com volumoso e concentrado extrusados em diferentes proporções de volumoso:concentrado (V:C) e (ii) a cinética de fermentação ruminal in vitro dessas rações. Foram utilizadas 20 fêmeas mestiças ½Dorper x ½Santa Inês recém-desmamadas com 120 ± 8 dias de idade. Os tratamentos foram quatro relações de volumoso extrusado (Foragge® ) e concentrado extrusado (Beef Total® ): 30%V:70%C, 40%V:60%C, 50%V:50%C e 60%V:40%C, distribuídos inteiramente ao acaso com cinco repetições. Os metabólitos energéticos analisados foram: VLDL, triglicerídeos e colesterol. Os metabólitos hepáticos foram: fosfatase alcalina, gama glutamiltransferase (GGT) e aspartatoaminotransferase (AST). Os metabólitos proteicos avaliados foram: proteínas totais, ácido úrico, ureia, creatinina, albumina e globulina. A quantificação da produção in vitro de gás foi feita com as rações extrusadas inoculadas em líquido ruminal de bovinos, avaliando as seguintes proporções volumoso:concentrado: 30%V:70%C, 40%V:60%C, 50%V:50%C, 60%V:40%C e 70%V:30%C. Os valores de cinética de fermentação foram comparados por meio do teste de paralelismo e identidade de curvas ao nível de 5% de probabilidade de erro. Para comparação das médias dos metabólitos, foi adotado estudo de regressão a 5 % de probabilidade de erro. Os dados médios de consumo de matéria seca são apresentados de forma descritiva. A ração extrusada provocou aumento nas enzimas hepáticas (GGT e AST), sem provocar lesões hepáticas e manteve estáveis os metabólitos energéticos. Similarmente, os níveis séricos proteicos permaneceram nos níveis normais recomendados para a espécie. O padrão de fermentação in vitro mostrou alta produção de gases associadas à alta digestibilidade da ração. A ração extrusada fornece aporte energético e proteico para atender às demandas nutricionais dos animais sem comprometer a sua saúde.

Extruded feed in different volume-concentrate ratios: in-vitro fermentation kinetics, consumption, and evaluation of lambs metabolic profile / Ração extrusada em diferentes relações de volumoso e concentrado: cinética de fermentação in vitro e avaliação do perfil metabólico de borregas

This study aimed both to evaluate the metabolic profile of lambs fed with extruded forage and concentrate containing different roughageconcentrate ratios (R:C) and to evaluate in-vitro ruminal fermentation kinetics of these rations. An experiment was carried out with 20 weanling, female crossbred lambs (½Dorper x ½Santa Inês) aged 120 ± 8 days. The treatments were four R:C ratios of extruded forage (Foragge®) and extruded concentrate (Beef Total®) 30%F:70%C, 40%F:60%C, 50%F:50%C, and 60%F:40%C distributed randomly with five replicates. The analysis targeted energy metabolites (VLDL, triglycerides, and cholesterol), hepatic metabolites (alkaline phosphatase, gamma glutamyltransferase [GGT], and aspartate aminotransferase [AST]), and protein metabolites (total protein, uric acid, urea, creatinine, albumin, and globulin). In-vitro gas production was quantified by inoculating the extruded rations in bovine ruminal fluid with the following forage:concentrate proportions evaluated: 30%F:70%C, 40%F:60%C, 50%F:50%C, 60%F:40%C and 70%F:30%C. Fermentation kinetic values were compared using parallelism test and curve identity at the error probability level of 5%. A regression study was used to compare the means of the metabolites at the error probability level of 5%. Average dry matter intake data are provided descriptively. The extruded ration increased the hepatic metabolites (GGT and AST) without compromising the liver and kept the energy metabolites stable. Similarly, the serum concentration of protein metabolites remained at normal levels recommended to the ovine species. In-vitro fermentation pattern displayed high gas production associated with high ration digestibility. The extruded ration provides protein and energy supply to meet the animals nutritional requirements without compromising their health.(AU)

Objetivou-se avaliar (i) o perfil metabólico de borregas alimentadas com volumoso e concentrado extrusados em diferentes proporções de volumoso:concentrado (V:C) e (ii) a cinética de fermentação ruminal in vitro dessas rações. Foram utilizadas 20 fêmeas mestiças ½Dorper x ½Santa Inês recém-desmamadas com 120 ± 8 dias de idade. Os tratamentos foram quatro relações de volumoso extrusado (Foragge® ) e concentrado extrusado (Beef Total® ): 30%V:70%C, 40%V:60%C, 50%V:50%C e 60%V:40%C, distribuídos inteiramente ao acaso com cinco repetições. Os metabólitos energéticos analisados foram: VLDL, triglicerídeos e colesterol. Os metabólitos hepáticos foram: fosfatase alcalina, gama glutamiltransferase (GGT) e aspartatoaminotransferase (AST). Os metabólitos proteicos avaliados foram: proteínas totais, ácido úrico, ureia, creatinina, albumina e globulina. A quantificação da produção in vitro de gás foi feita com as rações extrusadas inoculadas em líquido ruminal de bovinos, avaliando as seguintes proporções volumoso:concentrado: 30%V:70%C, 40%V:60%C, 50%V:50%C, 60%V:40%C e 70%V:30%C. Os valores de cinética de fermentação foram comparados por meio do teste de paralelismo e identidade de curvas ao nível de 5% de probabilidade de erro. Para comparação das médias dos metabólitos, foi adotado estudo de regressão a 5 % de probabilidade de erro. Os dados médios de consumo de matéria seca são apresentados de forma descritiva. A ração extrusada provocou aumento nas enzimas hepáticas (GGT e AST), sem provocar lesões hepáticas e manteve estáveis os metabólitos energéticos. Similarmente, os níveis séricos proteicos permaneceram nos níveis normais recomendados para a espécie. O padrão de fermentação in vitro mostrou alta produção de gases associadas à alta digestibilidade da ração. A ração extrusada fornece aporte energético e proteico para atender às demandas nutricionais dos animais sem comprometer a sua saúde.(AU)

Biodiesel co-products modified the rumen parameters of feedlot lambs but did not change methane production in vitro

Background: Intensification of livestock is a strategy that increases productivity, but the diets used to increase animal production efficiency are composed mainly of corn and soybean, thereby increasing competition between animals and humansfor the same food crops. This study evaluated nutrient intake, apparent digestibility of dry matter (DM) and nutrients, kinetics of gas production, and concentration of volatile fatty acids on diets with or without inclusion of biodiesel co-productsformulated for feedlot lambs. So, the hypothesis is that replace of traditional ingredients by biodiesel co-products changesrumen parameters and methane emissions.Materials, Methods & Results: The experiment was developed in São Paulo State University (Unesp), Jaboticabal, SP,Brazil, in Sheep Production Laboratory, which is owned to Animal Science Department. All trials developed in this studyused a feedlot system, where animals were kept in individual pen. Forty Ile de France lambs male non-castrated wereused in in vivo trial. To obtain rumen fluid, that was used in in vitro trial, four Santa Inês lambs with rumen cannula wereused. The treatments evaluated were four diets: Control diet: roughage + concentrate; PM20: roughage + concentrate withpeanut meal (PM) at 20% of DM; CG25: roughage + concentrate with crude glycerin (CG) at 25% of DM; and PMCG:roughage + concentrate with PM at 10% of DM and CG at 12.5% of DM. The roughage:concentrate ratio was 40:60 for allthese diets. The parameters of the in vitro and in vivo experiments used were completely randomized with four treatments.When significant, the means between treatments were compared using Tukey test (P < 0.05). There was no effect of coproduct inclusion on intake, except ether extract and neutral detergent fiber, which were higher for PM20 compared withCG25 diet. Apparent digestibility of dry matter (79.87%) and some nutrients (organic matter, crude protein, and neutraldetergent fiber)...(AU)

Biodiesel co-products modified the rumen parameters of feedlot lambs but did not change methane production in vitro

Background: Intensification of livestock is a strategy that increases productivity, but the diets used to increase animal production efficiency are composed mainly of corn and soybean, thereby increasing competition between animals and humansfor the same food crops. This study evaluated nutrient intake, apparent digestibility of dry matter (DM) and nutrients, kinetics of gas production, and concentration of volatile fatty acids on diets with or without inclusion of biodiesel co-productsformulated for feedlot lambs. So, the hypothesis is that replace of traditional ingredients by biodiesel co-products changesrumen parameters and methane emissions.Materials, Methods & Results: The experiment was developed in São Paulo State University (Unesp), Jaboticabal, SP,Brazil, in Sheep Production Laboratory, which is owned to Animal Science Department. All trials developed in this studyused a feedlot system, where animals were kept in individual pen. Forty Ile de France lambs male non-castrated wereused in in vivo trial. To obtain rumen fluid, that was used in in vitro trial, four Santa Inês lambs with rumen cannula wereused. The treatments evaluated were four diets: Control diet: roughage + concentrate; PM20: roughage + concentrate withpeanut meal (PM) at 20% of DM; CG25: roughage + concentrate with crude glycerin (CG) at 25% of DM; and PMCG:roughage + concentrate with PM at 10% of DM and CG at 12.5% of DM. The roughage:concentrate ratio was 40:60 for allthese diets. The parameters of the in vitro and in vivo experiments used were completely randomized with four treatments.When significant, the means between treatments were compared using Tukey test (P < 0.05). There was no effect of coproduct inclusion on intake, except ether extract and neutral detergent fiber, which were higher for PM20 compared withCG25 diet. Apparent digestibility of dry matter (79.87%) and some nutrients (organic matter, crude protein, and neutraldetergent fiber)...

Termination of lambs fed concentrate or pasture: performance and technical feasibility / Terminação de cordeiros alimentados com concentrado ou pastagem: desempenho e viabilidade técnica

A total of 28 uncastrated lambs, with 20kg of body mass (BM) were distributed into two different finishing systems where animals were fed either concentrate (CFS) or pasture (PFS) until 35 kg BM. The CFS diet consisted of 77.4% of corn grain; 20.2% soybean meal; 1.4% limestone and 1.0% sodium bicarbonate. The PFS diet consisted of both native pasture and ryegrass. The evaluated parameters were: dry matter intake (DMI), organic matter intake (OMI), crude protein intake (CPI), neutral detergent fiber intake (NDFI), feed conversion (FC), mean daily gain, days to reach 35kg BM, ruminal pH, in vivo biometric measurements, carcass composition, percentage of commercial cuts, pallet composition, and proportion of body components. The CFS animals had higher DMI, OMI, CPI and lower NDFI when compared to PFS. Also, animals feed CFS presented higher mean daily gain and better FC. Animals from CFS took 77 days to reach 35kg BM whereas animals from PFS took 278 days. The ruminal pH was higher, and more adequate, in the PFS group when compared to the CFS group. The CFS lambs presented better quantitative characteristics and conformation, and higher fat deposition. PFS lambs, on the other hand, provided leaner and more muscular carcasses. Thus, the termination of lambs without use of bulky feed is technically feasible and provides better zootechnical performance and carcass characteristics. However, the ruminal pH drop may compromise the system and must be observed.(AU)

Conduziu-se ensaio biológico com 28 cordeiros não castrados, com massa corporal (MC) inicial de 20 kg, distribuídos em dois diferentes sistemas alimentares de terminação: concentrado (STC) ou pastagem (STP). A dieta do STC foi constituída de 77,4% de grãos de milho; 20,2% de farelo de soja; 1,4% de calcário e 1,0% de bicarbonato de sódio. A dieta do STP foi constituída de pastagem nativa e azevém. Os animais permaneceram nos sistemas até atingirem 35 kg de MC. Os parâmetros avaliados foram: consumo de matéria seca (CMS), consumo de matéria orgânica (CMO), consumo de proteína bruta (CPB), consumo de fibra em detergente neutro (CFDN), conversão alimentar (CA), ganho médio diário, dias para atingir 35kg, pH ruminal, medidas biométricas in vivo, composição de carcaça, porcentagem de cortes comerciais, composição da paleta e proporção de componentes corporais. Os animais do SFC apresentaram maior CMS, CMO, CPB e menor CFDN quando comparados com o STP. Ainda os animais do STC apresentaram maior ganho médio diário e melhor CA. Os animais do STC levaram 77 dias para atingir 35kg de MC, enquanto os animais do STP levaram 278 dias. O pH ruminal foi maior e mais adequado no STP que no STP. Os cordeiros do STC apresentaram melhores características quantitativas e conformação, e maior deposição de gordura. Por outro lado, o STP forneceu carcaças mais magras e mais musculosas. Assim, a terminação de cordeiros sem utilização de ração volumosa é tecnicamente viável e proporciona melhor desempenho zootécnico e características de carcaça. Entretanto, a queda do pH ruminal pode comprometer o sistema e deve ser observada.(AU)

Milk fatty acid composition in Holstein x Gyr dairy cows fed chopped elephantgrass-based diets containing two types of sunflower oil associated with two methods of concentrate feeding / Perfil de ácidos graxos do leite de vacas Holandês x Gir alimentadas com dietas à base de capim-elefante picado, dois tipos de óleo de girassol e dois modos de fornecimento do concentrado

Two experiments were carried out in a 2 x 2 factorial arrangement with the objective of evaluating two methods of concentrate feeding for Holstein x Gyr cows fed 600 g kg-1 chopped elephantgrass-based diets supplemented at 45 g kg-1 DM with two types of sunflower oil (SO). The types of SO differed in the levels of oleic and linoleic fatty acids (FAs): high oleic/low linoleic acid – HO (73 and 10 g 100 g-1 FA, respectively) and medium oleic/medium linoleic acid – MO (43 and 34 g 100 g-1 FA, respectively). The concentrates containing HO SO or MO SO were supplied separately from the forage twice a day after the two milkings (TAD) or as part of a total mixed ration (TMR). In Experiment 1, a 4 x 4 Latin square design was used to evaluate the ruminal fermentation and degradation parameters in four rumen-cannulated cows (430±39 kg; 79±20 days in milk; 16.4±3.1 kg day-1 of milk). In Experiment 2, a randomized block design was used to evaluate the nutrient intake, plasma contents of metabolites and FAs, milk yield and composition, and FA profile of milk fat in 32 cows (444±84 kg; 75±31 days in milk; 15.4±4.8 kg day-1 of milk). The results were analyzed by mixed models (P < 0.05). The TMR diets promoted higher nutrient intake and rumen fermentation (higher ammonia N, acetate, propionate and total volatile FA contents) without affecting milk, fat, protein and lactose yields. TAD-fed cows presented higher feed efficiency and produced milk fat with a more nutritionally desirable FA composition, with higher vaccenic and rumenic acid contents and lower trans-10 C18:1 and palmitic acid contents. The DM intake, parameters of rumen fermentation and milk, fat, protein and lactose yields were similar for the HO SO and MO SO diets. The most nutritionally positive characteristics for human health in the milk fat of HO SO-fed cows were the higher eicosapentaenoic (+34%) and oleic acid (+11%) contents and lower palmitic acid content (-10%). Higher contents of vaccenic (+71%) and rumenic (+74%) acids and lower trans-10 C18:1 (-10%), elaidic (-32%), lauric (-14%) and myristic (-11%) acid contents were the most positive aspects of the milk fat of MO SO-fed cows. Considering the magnitudes of the differences in the levels of these FAs, it is concluded that the milk fat of cows fed MO SO showed a healthier milk FA profile.

Dois experimentos foram realizados em esquema de arranjo fatorial 2 x 2, com o objetivo de avaliar dois métodos de fornecimento de concentrado para vacas Holandês x Gir recebendo dietas à base de 600 g kg-1 de capim-elefante picado, suplementadas com dois tipos de óleo de girassol (OG) na base de 45 g kg-1 da matéria seca (MS) da dieta. Os OG diferiram por apresentar teores contrastantes dos ácidos graxos (AG) oleico e linoleico: alto teor de ácido oleico/baixo teor de ácido linoleico – HO (respectivamente, 73 e 10 g 100 g-1 de AG) e teores médios dos ácidos oleico e linoleico – MO (respectivamente, 43 e 34 g 100 g-1 de AG). Os concentrados contendo os OG foram fornecidos separadamente da forragem, duas vezes ao dia, após as duas ordenhas (TAD), ou como parte de uma dieta completa (TMR). No experimento 1, utilizou-se delineamento quadrado latino 4 x 4 para avaliar a fermentação e degradação ruminal em quatro vacas canuladas no rúmen (430±39 kg; 79±20 dias em lactação; 16,4±3,1 kg dia-1 de leite). No experimento 2, foi utilizado delineamento em blocos casualizados para avaliar o consumo de nutrientes, as concentrações plasmáticas de metabólitos e AG, e a produção, composição e o perfil de AG do leite em 32 vacas (444±84 kg; 75±31 dias em lactação; 15,4±4,8 kg dia-1 de leite). Os resultados foram analisados por modelos mistos (P < 0,05). As dietas fornecidas como TMR promoveram maior ingestão de nutrientes e fermentação ruminal (maiores teores de N amoniacal, acetato, propionato e AG voláteis totais), mas sem efeito nas produções de leite, gordura, proteína e lactose. As vacas que receberam concentrado duas vezes ao dia apresentaram maior eficiência alimentar e produziram leite com perfil de AG mais saudável, com maiores teores dos ácidos vacênico e rumênico, e menores de C18:1 trans-10 e ácido palmítico. O tipo de OG não influenciou a ingestão de MS, a fermentação ruminal e as produções de leite, gordura, proteína e lactose. As características nutricionais mais positivas na gordura do leite das vacas que receberam OG HO foram os maiores teores dos ácidos eicosapentaenoico (+34%) e oleico (+11%) e o menor teor de ácido palmítico (-10%). Maiores teores dos ácidos vacênico (+71%) e rumênico (+74%) e menores dos ácidos elaídico (-32%), láurico (-14%), mirístico (-11%) e C18:1 trans-10 (-10%) foram os aspectos nutricionais mais positivos na gordura do leite das vacas alimentadas com OG MO. Considerando as magnitudes das diferenças nos teores desses AG, conclui-se que a gordura do leite das vacas que receberam o OG MO apresentou perfil de AG mais saudável.