Recent decline in sperm motility among donor candidates at a sperm bank in Denmark.

STUDY QUESTION: Has there been variation in semen quality among men applying to be sperm donors (i.e. donor candidates) in Denmark in recent years (2017-2022)? SUMMARY ANSWER: The motile sperm concentration and total motile sperm count (TMSC) in ejaculates-both measures of sperm quality-declined by as much as 22% from 2019 to 2022. WHAT IS KNOWN ALREADY: Questions remain about whether human semen quality has declined in recent years. Whilst some studies provide evidence for a decline in human semen quality, these findings have been disputed owing to potential biases in the populations studied or in the methods used to measure semen quality. Resolution of this issue has important implications for human fertility, as well as for those involved in the recruitment of sperm donors for use in medically assisted reproduction. STUDY DESIGN, SIZE, DURATION: We obtained data on the semen quality of ejaculates previously collected from 2017 to 2022 at sperm bank locations in four cities in Denmark: Aarhus, Aalborg, Copenhagen, and Odense. Our study focuses on the single semen samples provided by 6758 donor candidates aged between 18 and 45 years old to determine whether their sperm quality met a minimum criterion for them to be accepted as sperm donors. PARTICIPANTS/MATERIALS, SETTING, METHODS: All ejaculates were analyzed within 1 hour of production. Semen volume (ml) was estimated by weight and both the concentration (106/ml) of sperm as well as the concentration of motile sperm (World Health Organization grades a and b) were measured using the same protocols and computer-assisted semen analysis system across all years at each site. Statistical analyses of the semen variables were controlled for age and donation site, as well as the average monthly high temperature when the ejaculate was produced. MAIN RESULTS AND THE ROLE OF CHANCE: From 2017 to 2019, semen volume, sperm concentration, and total sperm count in the ejaculates of donor candidates increased by 2-12%. Then, from 2019 to 2022, sperm concentration and total sperm count changed by 0.1-5% from year to year, but none of those changes were statistically significant. In contrast, both motile sperm concentration and TMSC declined significantly, by 16% and 22%, respectively, between 2019 and 2022. Thus, the concentration of motile sperm in donor candidates declined from 18.4 [95% CL: 17.0, 20.0] million/ml in 2019 to 15.5 [14.4, 16.7] million/ml in 2022, and TMSC declined from 61.4 [55.8, 67.5] million per ejaculate in 2019 to 48.1 [44.1, 52.4] million in 2022. LIMITATIONS, REASONS FOR CAUTION: We cannot determine from the available data the causes of the decline in semen quality of donor candidates from 2019 to 2022. However, as this period coincides with lockdowns and changes in work patterns during the coronavirus disease 2019 pandemic, it is possible that changes in motile sperm concentration and TMSC were the result of changes in the lifestyles of the men whose semen was analyzed. WIDER IMPLICATIONS OF THE FINDINGS: Men providing initial semen samples at sperm banks, when applying to be sperm donors, are a useful population in which to monitor changes in human semen quality over time. Our results have implications for human fertility and the recruitment of sperm donors for medically assisted reproduction, where motile sperm concentration is an essential selection criterion because it influences fertility. We suggest that gathering health and lifestyle data on donor candidates at sperm banks might help to identify causal factors for the decline of sperm quality that could be addressed and intervention, if desired, could be personalized for each accepted donor. STUDY FUNDING/COMPETING INTEREST(S): No external funding was obtained for this study. E.L. and A.-B.S. are employees of Cryos International. AP reports paid consultancy for Cryos International, Cytoswim Ltd, Exceed Health, and Merck Serono in the last 2 years of this study, but all monies were paid to the University of Sheffield (former employer). AP is also an unpaid trustee of the Progress Educational Trust (Charity Number 1139856). RM declares support from Cryos International to present results of this research at ESHRE 2023. None of the authors were directly involved in the collection or physical analysis of semen samples. TRIAL REGISTRATION NUMBER: N/A.

Low-Fat, High-Carbohydrate Diets Reduce Body Weight and Sperm Count but Increase Sperm Motility in Mice.

BACKGROUND: Male reproduction is impacted by both over- and under-nutrition, demonstrated by animal studies using high-fat and low-protein dietary interventions. Little is known about the impacts of low-fat, high-carb diets and types of dietary carbohydrates on sperm traits. OBJECTIVES: Using a nutritional geometry approach, we investigated the effects of partially or completely substituting glucose for fructose in isocaloric diets containing either 10%, 20%, or 30% fat (by energy) on sperm traits in mice. METHODS: Male C57BL/6J mice were fed 1 of 15 experimental diets for 18 wk starting from 8 wk of age. Reproductive organs were then harvested, and sperm concentration, motility, and velocity were measured using Computer-Assisted Sperm Analysis. RESULTS: Increasing dietary fat from 10% to 30% while maintaining energy density at 14.3 kJ/g and protein content at 20% resulted in increased body weight and sperm production but reduced the percentage of motile sperm. Body weight and seminal vesicle weight were maximized on diets containing a 50:50 mix of fructose and glucose, but carbohydrate type had few significant impacts on epididymal sperm traits. CONCLUSIONS: The opposing impacts of dietary fat on mouse sperm quantity and quality observed suggest that male fertility may not be optimized by a single diet; rather, context-specific dietary guidelines targeted to specific concerns in semen quality may prove useful in treating male infertility.

Nocturnal vestibular stimulation using a rocking bed improves a severe sleep disorder in a patient with mitochondrial disease.

Mitochondrial diseases are rare genetic disorders often accompanied by severe sleep disorders. We present the case of a 12-year-old boy diagnosed with a severe primary mitochondrial disease, exhibiting ataxia, spasticity, progressive external ophthalmoplegia, cardiomyopathy and severely disrupted sleep, but no cognitive impairment. Interestingly, his parents reported improved sleep during night train rides. Based on this observation, we installed a rocking bed in the patient's bedroom and performed different interventions, including immersive multimodal vestibular, kinesthetic and auditory stimuli, reminiscent of the sensory experiences encountered during train rides. Over a 5-month period, we conducted four 2-week nocturnal interventions, separated by 1-week washout phases, to determine the subjectively best-perceived stimulation parameters, followed by a final 4-week intervention using the optimal parameters. We assessed sleep duration and quality using the Mini Sleep Questionnaire, monitored pulse rate changes and used videography to document nocturnal interactions between the patient and caregivers. Patient-reported outcome measures, clinical examinations and personal outcomes of specific interests were used to document daytime sleepiness, restlessness, anxiety, fatigue, cognitive performance and physical posture. In the final 4-week intervention, sleep duration increased by 25%, required caregiver interactions reduced by 75%, and caregiving time decreased by 40%. Subjective fatigue, assessed by the Checklist Individual Strength, decreased by 40%, falling below the threshold of severe fatigue. Our study suggests that rocking beds could provide a promising treatment regime for selected patients with persistent severe sleep disorders. Further research is required to validate these findings in larger patient populations with sleep disorders and other conditions.

Improving the science and practice of using artificial roosts for bats.

Worldwide, artificial bat roosts (e.g., bat boxes, bark mimics, bat condos) are routinely deployed for conservation, mitigation, and community engagement. However, scant attention has been paid to developing best practices for the use of artificial roosts as conservation tools. Although bats readily occupy artificial roosts, occupancy and abundance data are misleading indicators of habitat quality. Lacking information on bat behavior, health, and fitness in artificial roosts, their conservation efficacy cannot be adequately validated. We considered the proximal and ultimate factors, such as evolutionarily reliable cues, that may prompt bats to preferentially use and show fidelity to suboptimal artificial roosts even when high-quality alternatives are available. Possible negative health and fitness consequences for artificial roost inhabitants include exposure to unstable and extreme microclimates in poorly designed roosts, and vulnerability to larger numbers of ectoparasites in longer lasting artificial roosts that house larger bat colonies than in natural roosts. Bats using artificial roosts may have lower survival rates if predators have easy access to roosts placed in conspicuous locations. Bats may be lured into occupying low-quality habitats if attractive artificial roosts are deployed on polluted urban and agricultural landscapes. To advance the science behind artificial bat roosts, we present testable research hypotheses and suggestions to improve the quality of artificial roosts for bats and decrease risks to occupants. Because continued loss of natural roosts may increase reliance on alternatives, such as artificial roosts, it is imperative that this conservation practice be improved.

Mejoras en la ciencia y práctica del uso de perchas artificiales para murciélagos Resumen Las perchas artificiales para murciélagos (cajas, imitaciones de corteza, condominios) se usan en todo el mundo para la conservación, mitigación y participación de la comunidad. Sin embargo, se ha prestado poca atención al desarrollo de las mejores prácticas en cuanto al uso de perchas artificiales como herramientas de conservación. Aunque los murciélagos ocupan fácilmente las perchas artificiales, los datos de su abundancia y ocupación son indicadores engañosos de la calidad del hábitat. La falta de información sobre el comportamiento, salud y adaptabilidad de los murciélagos en las perchas artificiales causa que no pueda validarse adecuadamente la eficiencia de conservación de las perchas. Consideramos los factores proximales y finales, como las pautas de confianza evolutiva, que pueden llevar a los murciélagos a usar con preferencia y mostrar fidelidad a las perchas artificiales subóptimas cuando existen alternativas de gran calidad. Las posibles consecuencias negativas para la salud y adaptabilidad de los habitantes de los nidos artificiales incluyen la exposición a microclimas inestables y extremos en perchas mal diseñadas y la vulnerabilidad a un mayor número de ectoparásitos en perchas artificiales de mayor duración que albergan colonias mayores a las perchas naturales. Los murciélagos que usan perchas artificiales pueden tener tasas menores de supervivencia si los depredadores tienen acceso fácil a las perchas colocadas en ubicaciones conspicuas. Puede que los murciélagos terminen ocupando hábitats de baja calidad si se construyen perchas artificiales atractivas sobre terrenos agrícolas y urbanos contaminados. Presentamos hipótesis comprobables de investigación y sugerencias para mejorar la calidad de las perchas artificiales y reducir los riesgos para sus habitantes y así avanzar la ciencia detrás de las perchas artificiales para murciélagos. Ya que la pérdida continua de perchas naturales puede incrementar la dependencia de alternativas, como las perchas artificiales, es imprescindible que se mejore esta práctica de conservación.

BBOF1 is required for sperm motility and male fertility by stabilizing the flagellar axoneme in mice.

The sperm flagellum is a specialized type of motile cilium composed of a typical "9 + 2" axonemal structure with peri-axonemal structures, such as outer dense fibers (ODFs). This flagellar arrangement is crucial for sperm movement and fertilization. However, the association of axonemal integrity with ODFs remains poorly understood. Here, we demonstrate that mouse BBOF1 could interact with both MNS1, an axonemal component, and ODF2, an ODF protein, and is required for sperm flagellar axoneme maintenance and male fertility. BBOF1 is expressed exclusively in male germ cells from the pachytene stage onwards and is detected in sperm axoneme fraction. Spermatozoa derived from Bbof1-knockout mice exhibit a normal morphology, however, reduced motility due to the absence of certain microtubule doublets, resulting in the failure to fertilize mature oocytes. Furthermore, BBOF1 is found to interact with ODF2 and MNS1 and is also required for their stability. Our findings in mice suggest that Bbof1 could also be essential for human sperm motility and male fertility, thus is a novel potential candidate gene for asthenozoospermia diagnosis.

Bioclimatic thermal stress indices and their relationships with andrological characteristics in hair rams.

This study evaluated relationships among reproductive parameters and the bioclimatic indices: temperature and humidity index (THI), equivalent temperature index (ETI), black globe temperature and humidity index (BGTHI), and thermal comfort index (TCI), during the first 45 days of spermatogenesis (SP-45) and during the 15 days of sperm transit through the epididymis (STP-15) that preceded the reproductive assessments (ReA). Such information is useful in determining the optimal breeding season in Northeast Brazil. Santa Inês rams (n = 25) underwent two ReA in three periods of the year (D-P = dry; R-P = rainy and RD-P = rainy/dry transition), and the bioclimatic indices were calculated at the corresponding SP-45 and STP-15 timepoints prior to each ReA. Sperm kinetic parameters in D-P were depressed compared to R-P and RD-P (P < 0.05). The index values had an antagonistic relationship with most parameters and regression analysis demonstrated that the BGTHI and the TCI had a negative association with the progressive motility, curvilinear, straight line, and average path velocities, and a positive association with slow sperm in the ejaculate in SP-45 and STP-15 phases (P < 0.01). Semen quality kinetics is affected throughout the year by the environment and it is apparent that it is impaired in D-P and better in R-P and RD-P seasons. The BGTHI and TCI measured in the sperm production phase classified the environment most coherently and presented better association with the behavior of sperm kinetics.

Investigating net primary production in climate regions of Khuzestan Province, Iran using CASA model.

This study aimed to investigate the vegetation production changes in Khuzestan province, Iran using MODIS data production, meteorological data, vegetation maps as well as topographic and field monitoring data in CASA model. The study area was divided into different climatic classes based on multivariate statistical method, so the vegetation of each climatic region was examined separately for changes in NPP values. Production changes due to degradation were calculated using the Miami model and subsequently, the rain use efficiency (RUE) and the light use efficiency (LUE) and correlation indices between the CASA model and ground data were determined. The results of this study (R2) showed that the accuracy of this model varies depending on the type of climatic regions (R2 = 0.80 to R2 = 0.15). In different climatic regions, the rate of NPP changes (very humid 68 gC/m2 to ultra-dry 15 gC/m2) varies in rangeland types. The highest rate of vegetation production is observed seasonally in May. Degradation conditions also reduced RUE and LUE. However, in hyper-arid regions, adaptations of plants in some different species (Hammada Spp.) increase their efficiency compared to other vegetation types. The results showed the importance of vegetation and climate classification in vegetation production studies.

Comparison of computer-assisted sperm analysis and smartphone-applied sperm analysis for evaluation of frozen-thawed bull semen.

This study aims to compare the efficacy of computer-assisted sperm analysis (CASA) and smartphone-applied sperm analysis (SASA) in assessing the quality of frozen-thawed bull semen. A total of 75 straws (n = 75) semen samples were used from different production batches of five Holstein bulls. The semen analyses were conducted in three groups: Group I (CASA-37°C), semen samples were evaluated using the CASA system at 37°C (n = 25); Group II (SASA-25°C), semen samples were assessed using the SASA system at a temperature of room heat (25°C) (n = 25); and Group III (SASA-37°C), semen samples were evaluated using the SASA system at 37°C (n = 25). The frozen-thawed bull semen samples were analysed in terms of total motility (TM), progressive motility (PM), immotile, velocity average path (VAP), velocity curve linear (VCL), velocity straight line (VSL) and sperm concentration. There was no significant difference between the groups in terms of spermatozoa concentration (p > .05). However, significant differences among the groups were observed for total motile spermatozoa values (p < .001). Values of progressive motile spermatozoa were lower in Group I and Group II compared to Group III (p < .001). The immotile spermatozoa values were significant between the groups (p < .001) and were found to be proportional to total motile spermatozoa values. Additionally, the VAP, VCL and VSL values were comparable between Group II and Group III, but lower when compared to Group I. In conclusion, the results of the study demonstrate that the Sperm Cell™ system can accurately analyse the concentration of frozen-thawed bull semen. The analyses performed at room temperature indicate a parallelism between the PM value and CASA results. However, it is thought that SASA devices require a series of standardization studies in different semen extenders, different sample concentrations and different animal species, analogous to the standardization evolution process of CASA devices in semen analysis.

Quantitative identification on spatial mismatch characteristics between supply and demand of carbon sequestration services.

Exploring the mismatch between supply and demand (SD) for carbon sequestration services (CSS) is essential for achieving the "double carbon" goal. However, more studies are needed on the traits of the spatial mismatch between SD in mountainous cities. We used the CASA model and the IPCC emission factor approach to address this issue and quantify the SD of CSS in Chongqing. Second, we established a matching relationship model for the SD of CSS in Chongqing. Finally, we applied the Structural Equation Model with the Partial Dependence Plots model to reveal the influencing factors and internal mechanisms of spatial mismatch between the SD of CSS in Chongqing. The outcomes confirmed a decrease in fashion in the total supply of CSS in Chongqing and growth in fashion in general demand from 2000 to 2020. The SD mismatch was mainly concentrated inside the central city and other built-up areas. The SD mismatch area had increased by 390%, indicating a continuous upward trend. In exploring the factors influencing the mismatch between the SD of CSS in Chongqing, supply is mainly positively influenced by NDVI, and demand and supply-demand relationships are influenced by population density and LUCC. We proposed policy suggestions to alleviate the spatial mismatch and practical significance for achieving the "double carbon" goal and promoting sustainable development.

Evaluating net primary productivity dynamics and their response to land-use change in the loess plateau after the 'Grain for Green' program.

Assessing net primary productivity (NPP) dynamics and the contribution of land-use change (LUC) to NPP can help guide scientific policy to better restore and control the ecological environment. Since 1999, the "Green for Grain" Program (GGP) has strongly affected the spatial and temporal pattern of NPP on the Loess Plateau (LP); however, the multifaceted impact of phased vegetation engineering measures on NPP dynamics remains unclear. In this study, the Carnegie-Ames-Stanford Approach (CASA) model was used to simulate NPP dynamics and quantify the relative contributions of LUC and climate change (CC) to NPP under two different scenarios. The results showed that the average NPP on the LP increased from 240.7 gC·m-2 to 422.5 gC·m-2 from 2001 to 2020, with 67.43% of the areas showing a significant increasing trend. LUC was the main contributor to NPP increases during the study period, and precipitation was the most important climatic factor affecting NPP dynamics. The cumulative amount of NPP change caused by LUC (ΔNPPLUC) showed a fluctuating growth trend (from 46.23 gC·m-2 to 127.25 gC·m-2), with a higher growth rate in period ΙΙ (2010-2020) than in period Ι (2001-2010), which may be related to the accumulation of vegetation biomass and the delayed effect of the GGP on NPP. The contribution rate of LUC to increased NPP in periods Ι and ΙΙ was 101.2% and 51.2%, respectively. Regarding the transformation mode, the transformation of grassland to forest had the greatest influence on ΔNPPLUC. Regarding land-use type, the increased efficiency of NPP was improved in cropland, grassland, and forest. This study provides a scientific basis for the scientific management and development of vegetation engineering measures and regional sustainable development.

Desmin mutations impact the autophagy flux in C2C12 cell in mutation-specific manner.

Desmin is the main intermediate filament of striated and smooth muscle cells and plays a crucial role in maintaining the stability of muscle fiber during contraction and relaxation cycles. Being a component of Z-disk area, desmin integrates autophagic pathways, and the disturbance of Z-disk proteins' structure negatively affects chaperone-assisted selective autophagy (CASA). In the present study, we focused on alteration of autophagy flux in myoblasts expressing various Des mutations. We applied Western blotting, immunocytochemistry, RNA sequencing, and shRNA approach to demonstrate that DesS12F, DesA357P, DesL345P, DesL370P, and DesD399Y mutations. Mutation-specific effect on autophagy flux being most severe in aggregate-prone Des mutations such as DesL345P, DesL370P, and DesD399Y. RNA sequencing data confirmed the most prominent effect of these mutations on expression profile and, in particular, on autophagy-related genes. To verify CASA contribution to desmin aggregate formation, we suppressed CASA by knocking down Bag3 and demonstrated that it promoted aggregate formation and lead to downregulation of Vdac2 and Vps4a and upregulation of Lamp, Pink1, and Prkn. In conclusion, Des mutations showed a mutation-specific effect on autophagy flux in C2C12 cells with either a predominant impact on autophagosome maturation or on degradation and recycling processes. Aggregate-prone desmin mutations lead to the activation of basal autophagy level while suppressing the CASA pathway by knocking down Bag3 can promote desmin aggregate formation.

The impact of climate change and human activities to vegetation carbon sequestration variation in Sichuan and Chongqing.

Exploring the vegetation carbon cycle and the factors influencing vegetation carbon sequestration in areas with complex plateau-basin topography and fragile ecosystems is crucial. In this study, spatial and temporal characteristics of carbon sequestration by vegetation in Sichuan and Chongqing from 2010 to 2020 and the influencing factors were investigated through simulations of net primary productivity (NPP) using the modified Carnegie-Ames-Stanford approach (CASA) and the Thornthwaite Memorial (TM) model and using chemical equations of photochemical reactions. The results indicated that: The spatial distribution of carbon sequestration capacity (CSC) trends showed an increase in the east (the most prominent increased trend along the mountainous areas of the basin) and a decrease in the west (western Sichuan plateau). Differences exist in the impact factors of CSC in different regions. In the basin margins and mountainous areas, where the proportion of forests was high, a combination of climate change and human activities contributed to the increase in CSC. The relatively warm and humid meteorological conditions in the hinterland of the basin were more conducive to the increase in CSC, and climate change also affected the region more significantly. In contrast, in the relatively high altitude of western Sichuan, controlled human activities were the key to improving CSC. The results of the study contribute to the understanding of the basic theory of vegetation carbon cycle in areas with complex plateau-basin topography and fragile ecosystems, as well as to provide suggestions for ecological shelter construction and ecological restoration in the upper Yangtze River.

Cryopreservation efficiency of red-rumped agouti (Dasyprocta leporina) sperm obtained from different origins through epididymal retrograde flushing or electroejaculation.

This study investigated whether the origin of sperm (epididymal vs. ejaculate) affects the cryopreservation efficiency in agouti (Dasyprocta leporina). Five sexually mature agoutis underwent electroejaculation, resulting in obtaining four semen samples. After 15 days, the same animals were euthanized, and through retrograde flushing, sperm samples were obtained from the epididymis tails. In both collection methods, samples were evaluated for sperm parameters (sperm concentration, motility, vigor, membrane integrity, osmotic response, and morphology). Then, samples were diluted in ACP 109c, added with 20% egg yolk, and a final concentration of 6% glycerol. Finally, the samples were packaged in 0.25 mL straws and frozen in liquid nitrogen. After one week, samples were thawed and evaluated in the same way as fresh samples, with the addition of membrane integrity analysis using fluorescent probes (C-FDA/PI) and computerized analysis (CASA). Immediately after obtaining the sperm, samples obtained directly from the epididymis presented higher values (P ≤ 0.05) than those obtained by electroejaculation concerning the parameters of volume, sperm concentration, and total number of sperm (1,398.25 ± 206.0 x106 and 184.5 ± 78.0 x106 sperm). On the other hand, in the classical evaluation of the other sperm parameters and the computerized analysis (CASA) after thawing, such as total motility, no statistical differences were observed between sperm from both origins (ejaculate: 16.7 ± 8.2% and epididymal: 24.8 ± 12.0%, P > 0.05). This demonstrates the possibility of direct application of the cryopreservation protocol for agouti (D. leporina) sperm obtained via the epididymis or ejaculate.

Current cough and sputum assessed by the cough and sputum assessment-questionnaire (CASA-Q) is associated with quality of life impairment in cystic fibrosis.

BACKGROUND: Cough and sputum are major symptoms in cystic fibrosis (CF) that contribute to the impairment of quality of life. METHODS: This prospective single centre cross-sectional pilot study aimed to evaluate the results of a self-administered questionnaire assessing cough and sputum symptoms (2 domains), and their impact (2 domains) on daily activities in the previous week, named the Cough and Sputum Assessment Questionnaire (CASA-Q) in CF adult patients at stable state, and to analyse associations with clinical, functional, microbiological, radiological data, and two quality of life scales: the Cystic Fibrosis Questionnaire Revised (CFQ-R) and the Saint George Respiratory Questionnaire (SGRQ). RESULTS: Forty-eight patients were included in this analysis (69% men; median age of 27.8 ± 8.1 years; median body mass index of 21.8 + 3.3 kg/m²; mean FEV1 of 64 ± 30% of the predicted value). The mean values of the CASA-Q domains were 58 ± 23 for cough symptoms, 77 ± 24 for cough impact, 62 ± 25 for sputum symptoms and 84 ± 21 for sputum impact. Impairment in CASA-Q cough and sputum domains was associated with dyspnea mMRC scale (p < 0.005 for all 4 domains of CASA-Q) and exacerbations in the previous year (p < 0.05 for CASA-Q symptoms domains). We also found correlations between all domains of the CASA-Q and quality of life questionnaires including SGRQ (p < 0.001) and to a lesser extend CFQ-R. We identified a clinical phenotype (female gender, ΔF508 heterozygous mutation, dyspnea mMRC scale) associated with an impairment of CASA-Q score and quality of life using a 2-step cluster analysis. CONCLUSIONS: CASA-Q allows the assessment of cough and sputum in CF adult patients and is associated with quality of life impairment. This simple easy-to-use tool could be used in routine clinical practice and in clinical studies to assess cough and sputum in CF patients. TRIAL REGISTRATION: The study was registered on ClinicalTrials.gov (NCT02924818, first posted on 5th October 2016).

Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions.

In a previous study, our group detected the cholecystokinin (CCK) protein in the porcine oviduct. This fact, together with the involvement of CCK in the regulation of sperm protein tyrosine phosphorylation by the modulation of HCO3 - uptake (in mice and humans) suggests a role for CCK during sperm capacitation. Therefore, on the one hand, the expression of CCK receptors (CCK1R and CCK2R) on boar testes has been investigated and probed; on the other hand, boar spermatozoa (from seminal doses of 1-day and 5-day storage) were exposed to different concentrations of CCK (0-control, 25 or 50 µM) in a medium supporting capacitation supplemented with 0, 5 or 25 mmol/L of HCO3 - for 1 h at 38.5°C. Sperm motion (total and progressive motility), kinetic parameters, viability, acrosome status, and mitochondrial activity were determined. No differences between groups (0, 25 or 50 µM of CCK) were observed when HCO3 - was absent in the media (p > .05). However, the results showed that when the media was supplemented with 5 mmol/L HCO3 - in 1-day seminal dose storage, the linearity index (LIN, %), straightness index (STR, %) and oscillation index (WOB, %) (sperm kinetics parameters) increased in the presence of CCK regardless the concentration (p < .05). Nevertheless, CCK in sperm from 5-day storage only increased the WOB parameter in comparison to the control (p < .05). Furthermore, the average amplitude of the lateral displacement of the sperm head (ALH, µm) and curvilinear velocity (VCL, µm/s) decreased when CCK was present, depending on its concentration and sperm aging (1-day vs. 5-days) (p < .05). In the case of the media supporting capacitation supplemented with 25 mmol/L HCO3 - , any differences were observed except for sperm viability in the 5-day seminal doses, which increased in the 50 µM-CCK group compared to the control (p < .05). In conclusion, these data suggest an implication of CCK protein during sperm capacitation under low bicarbonate concentration increasing the sperm linear trajectory.

"You do need to do the interaction": Mothers' perceptions of responsive parenting following a home-based parenting intervention.

Responsive parenting (also known as responsivity) is a dynamic and bidirectional exchange between the parent-child dyad and associated with a child's social and cognitive development. Optimal interactions require a sensitivity and understanding of a child's cues, responsiveness to the child's need, and a modification of the parent's behavior to meet this need. This qualitative study explored the impact of a home visiting program on mothers' perceptions of their responsivity to their children. This study is part of a larger body of research known as right@home, an Australian nurse home visiting program promoting children's learning and development. Preventative programs such as right@home prioritize population groups experiencing socioeconomic and psychosocial adversity. They provide opportunities to promote children's development through the enhancement of parenting skills and an increase in responsive parenting. Semi-structured interviews were conducted with 12 mothers, providing insight into their perceptions of responsive parenting. Four themes were extracted from the data using inductive thematic analysis. These indicated that: (1) mothers' perceived preparation for parenting, (2) recognition of mother and child needs, (3) response to mother and child needs, and (4) motivation to parent with responsiveness, were considered important. This research highlights the importance of interventions that focus on the parent-child relationship in increasing mother's parenting capabilities and promoting responsive parenting.

La crianza con sensibilidad (también conocida como crianza con capacidad de respuesta) es un dinámico intercambio en ambas direcciones entre la díada progenitor-niño, asociada con el desarrollo social y cognitivo del niño. Las interacciones óptimas requieren de una sensibilidad y comprensión de las señales del niño, capacidad de respuesta a la necesidad del niño, y de una modificación del comportamiento del progenitor para cumplir con esta necesidad. Este estudio cualitativo exploró el impacto de un programa de visita a casa sobre las percepciones de las madres acerca de su capacidad de respuesta a sus niños. Este estudio es parte de un mayor cuerpo investigativo conocido como right@home (justo en casa), un programa australiano de visita a casa por parte de enfermeras que promueve el aprendizaje y desarrollo de los niños. Los programas preventivos tales como right@home dan prioridad a grupos de población que experimentan adversidad socioeconómica y sicosocial. Ellos ofrecen oportunidades para promover el desarrollo de los niños por medio del mejoramiento de las habilidades de crianza y un incremento de la sensibilidad en la crianza. Se llevaron a cabo entrevistas semiestructuradas con 12 madres, lo cual aportó ideas sobre sus percepciones de la crianza con sensibilidad. Se extrajeron cuatro temas de la información usando análisis temáticos inductivos. Estos indicaron que se consideraban importantes (1) el cómo las madres percibían su preparación para la crianza, (2) el reconocimiento de las necesidades de la madre y del niño, (3) la respuesta a las necesidades de la madre y del niño, y (4) la motivación a criar con sensibilidad. Esta investigación subraya la importancia de intervenciones que se enfoquen en la relación progenitor-niño para aumentar las capacidades de crianza de la madre y promover una crianza con sensibilidad.

Le parentage réactif (aussi connu comme réactivité) est un échange dynamique et bidirectionnel entre la dyade parent-enfant, et lié au développement social et cognitive d'un enfant. Les interactions optimales demandent une sensibilité et une compréhension des signaux de l'enfant, la réactivité au besoin de l'enfant, et une modification du comportement du parent pour répondre à ce besoin. Cette étude qualitative a exploré l'impact d'un programme de visite à domicile sur les perceptions des mères de leur réactivité à leurs enfants. Cette étude fait partie de recherches plus étendues connues sous le nom de de right@home, un programme de visite infirmières à domicile australien promouvant l'apprentissage et le développement des enfants. Les programmes de prévention tels que right@home privilégient les groupes de population marqués par l'adversité socioéconomique et psychosociale. Ils offrent des opportunités de promouvoir le développement des enfants au travers du renforcement des capacités de parentage et une augmentation du parentage réactif. Des entretiens semi-structurés ont été faits avec 12 mères, offrant un aperçu de leurs perceptions du parentage réactif. Quatre thèmes ont été extraits des données en utilisant une analyse thématique inductive. Ces dernières ont indiqué que (1) la préparation perçue des mères pour le parentage, (2) la reconnaissance des besoins de la mère et de l'enfant, (3) la réponse aux besoins de la mère et de l'enfant, et (4) la motivation du parent avec la réaction ont toutes été considérées comme importantes. Ces recherches mettent en lumière l'importance d'interventions qui se concentrent sur la relation parent-enfant en augmentant les capacités de parentage des mères et en promouvant le parentage réactif.

Pilot test of a measure to assess clients' perceptions of their home visitors.

Home visiting services for caregivers of infants and young children have been found to be an effective method for promoting child development. Unfortunately, such services suffer from high rates of attrition, suggesting the need to identify factors related to client retention in services. Research has indicated that the client-provider relationship is an important predictor of client retention in home visiting services; however, measures to assess this relationship are limited. This study reports on a pilot test of the Client Perceptions of Home Visitors Questionnaire, developed to assess home visiting clients' perceptions of their relationship with their home visitor. The measure was completed by 39 diverse home visiting clients during pregnancy in a midwestern town in the United States. The scale was found to have good internal consistency. Validity analyses found that the scale was positively associated with client satisfaction with services and perceived provider cultural sensitivity. The scale was also positively associated with retention in services at an 8-month follow-up. Additional research should examine the measure's psychometrics with larger samples.

Se ha determinado que los servicios de visita a casa para quienes prestan el cuidado a infantes y niños pequeños es un eficaz método para promover el desarrollo del niño. Desafortunadamente, tales servicios sufren de altas tasas de deserción, lo cual sugiere que hay una necesidad de identificar los factores relacionados con la retención del cliente en los servicios mencionados. La investigación ha señalado que la relación entre cliente y proveedor es un importante factor de predicción de la retención del cliente en los servicios de visita a casa; sin embargo, las medidas para evaluar esta relación son limitadas. Este estudio reporta acerca de un examen experimental del Cuestionario sobre las Percepciones del Cliente acerca de los Visitadores a Casa, desarrollado para evaluar las precepciones que tienen los clientes de visitas a casa acerca de su relación con el visitador a casa. Treinta y nueve clientes diversos de las visitas a casa completaron la medida durante el embarazo en un pueblo del medio oeste de los Estados Unidos. Se determinó que la escala tenía una buena consistencia interna. Los análisis de validez determinaron que la escala se asociaba positivamente con la satisfacción del cliente en cuanto a los servicios y la percibida sensibilidad cultural del proveedor. También se asoció la escala positivamente con la retención en los servicios al momento del seguimiento a los 8 meses. La investigación adicional debe examinar los aspectos sicométricos de la medida utilizando grupos muestras más grandes.

On considère que les services de visite à domicile pour les personnes prenant soin de bébés et de jeunes enfants sont une méthode efficace pour promouvoir le développement de l'enfant. Malheureusement de tes services souffrent de taux élevés d'attrition, suggérant donc le besoin d'identifier des facteurs liés à la rétention du client dans ces services. Les recherches ont indiqué que la relation client-prestataire est un facteur de prédiction important pour la rétention du client dans les services de visite à domicile. Cependant les mesures pour évaluer cette relation sont limitées. Cette étude porte sur un test pilote du Questionnaire des Perceptions du Client de la Visite à Domicile, développé afin d'évaluer les perceptions des clients de visite à domicile de leur relation au visiteur à domicile. La mesure a été remplie par 39 clients de visite a domicile, de milieux divers, durant la grossesse dans une ville du centre nord des Etats-Unis d'Amérique. On a trouvé que l'échelle avait une bonne consistance interne. Des analyses de validité ont trouvé que l'échelle était liée de manière positive à la satisfaction du client avec les services et à la sensibilité culturelle perçue du prestataire. L'échelle était aussi liée de manière positive à la rétention dans les services à un suivi à 8 mois. Des recherches supplémentaires devraient examiner la psychométrie de la mesure avec des échantillons plus grands.

What works for whom? Mother's psychological distress as a moderator of the effectiveness of a home visiting intervention.

Attachment-based home visiting programs that serve new mothers experiencing psychological distress may advance health equity by helping families systemically exposed to adversity. This study examined whether one such program (Promoting First Relationships/PFR) had particularly beneficial effects on maternal and child relationship outcomes for mothers reporting the greatest psychological distress. A randomized controlled trial of the PFR program included a low-income sample of 252 Spanish- and English-speaking mother-child dyads referred prenatally for mental health concerns. The sample of mothers was 65.5% White, 17.5% Black, and 17.1% multiracial or other racial groups; 47.2% reported Hispanic ethnicity. The moderating variable of psychological distress was measured using maternal-reported screening tools for symptoms of depression, anxiety, anger, post-traumatic stress, and interpersonal sensitivity. Outcomes included observed parenting sensitivity and self-reported understanding of infants/toddlers, caregiving confidence, and child externalizing behavior. Results showed a significant treatment condition by baseline psychological distress interaction for observed parenting sensitivity such that differences in outcomes favoring the PFR condition were greatest among those with high baseline psychological distress (baseline child age 6-12 weeks). In a low-income sample of new mothers, those with the greatest need, as indicated by high psychological distress, showed greater improvements in their sensitive and responsive caregiving if they were randomized to the PFR treatment condition.

Los programas de visita a casa basados en la afectividad que sirven a madres nuevas que experimentan angustia sicológica pudieran mejorar la equidad de salud ayudando a familias sistemáticamente expuestas a circunstancias adversas. Este estudio examinó si uno de tales programas (Promover Primeras Relaciones /PFR) tiene particularmente efectos beneficiosos en los resultados de la relación materna y del niño para madres que reportan la mayor angustia sicológica. Un ensayo controlado al azar sobre el programa PFR incluyó un grupo muestra de bajos recursos económicos de 252 díadas de madre-niño que hablaban español o inglés, referidas prenatalmente por razón de salud mental. El grupo muestra de madres estaba compuesto de 65.5% blancas, 17.5% negras, y 17.1% multirracial o de otros grupos raciales; el 47.2% reportó origen étnico hispano. La variable moderadora de angustia sicológica se midió con herramientas de detección reportadas por las madres para síntomas de depresión, ansiedad, ira, estrés postraumático y sensibilidad interpersonal. Entre los resultados se incluyen la observada sensibilidad de crianza y la auto-reportada comprensión de infantes y niños pequeñitos, la confianza en la prestación de cuidado, así como la conducta de externalización del niño. Los resultados muestran una significativa condición de tratamiento por medio de interacción de angustia sicológica al nivel básico para la observada sensibilidad de crianza, de manera que las diferencias en resultados que favorecen la condición PFR fueron mayores entre quienes presentaban una alta angustia sicológica de base (punto base edad del niño 6 a 12 semanas). En un grupo muestra de madres nuevas de bajos recursos económicos, aquellas con las mayores necesidades, tal como indica la alta angustia sicológica, mostraron mayores logros en su sensibilidad y el cuidado sensible si se les había seleccionado al azar para la condición de tratamiento PFR.

Les programmes de visite à domicile basés sur l'attachement qui servant des nouvelles mères faisant l'expérience de détresse psychologique peuvent faire avancer l'équité en santé en aidant des familles systématiquement exposées à l'adversité. Cette étude a examiné si un tel programme (la Promotion de Premières Relations, soit en anglais Promoting First Relationships/PFR) avait des effets particulièrement bénéfiques sur les résultats de relation maternelle et enfant pour les mères faisant état de la plus grande détresse psychologique. Un essai contrôlé randomisé du programme PFR a inclus un échantillon de 252 dyades mères-enfants de milieu défavorisé, parlant espagnol et anglais, référées avant la naissance pour des problèmes de santé mentale. L'échantillon de mères était 65,5% caucasiennes/blanches, 17,5% noires, and 17,1% multiraciales ou autres groupes raciaux, 47,2% faisant état d'une ethnicité hispanique. La variable modératrice de détresse psychologique a été mesurée en utilisant des outils de dépistage rapportés par la mère de dépression, d'anxiété, de stress post-traumatique, et de sensibilité interpersonnelle. Les résultats ont inclus une sensibilité de parentage observée et une compréhension auto-rapportée des bébés/petits enfants, une confiance de parentage, et un comportement externalisant de l'enfant. Les résultats montrent une condition de traitement importante par l'interaction psychologique de base pour la sensibilité de parentage observée telles que les différences dans les résultats favorisant la condition PFR étaient les plus grandes parmi celles avec la base de détresse psychologique élevée (âge de base de l'enfant 6-12 semaines). Chez un échantillon de nouvelles mères de milieux défavorisés, celles ayant le besoin le plus élevé, indiqué par une haute détresse psychologique, ont fait preuve des plus améliorations dans leur parentage sensible et réactif si elles étaient randomisées pour la condition de traitement PFR.

Facilitating caregiver-child interactions in home visiting: A qualitative observational study.

Facilitating parent-child interactions is a key component of evidence-based early childhood home visiting programs. Several observational measures have been created to effectively capture home visiting activities from an operationalized perspective. While the field has a reasonable understanding of what home visitors can do to facilitate developmentally supportive interactions, and why, the how remains elusive for many home visitors. This qualitative study used a modified grounded theory approach to examine the facilitation of caregiver-child interactions during home visits. Home visit video recordings previously coded using the Home Visit Rating Scale (HOVRS) A+ were selected based on facilitation quality. Sixteen home visits (8 lower- and 8 higher-quality facilitation scores) were examined for differences in home visitor behaviors that support parent-child interactions during visits. We identified contextual and specific behaviors that home visitors used to actively engage caregivers and children together in developmentally supportive interactions. Results revealed 11 home visitor behaviors that discriminated higher quality facilitation. These behaviors were categorized into four domains: environmental conditions, executing the activity, strengths-based focus, and caregiver responses. This qualitative study highlights specific behaviors and professional development areas to support more effective home visiting services for a diverse population of families with young children.

Facilitar las interacciones progenitor-niño es un componente clave de los programas de visita a casa en la temprana niñez con base en la evidencia. Varias medidas de observación se han creado para captar eficazmente las actividades de visita a casa desde una perspectiva operacional. A pesar de que el campo tiene una comprensión razonable de lo que los visitantes a casa pueden hacer para facilitar las interacciones de apoyo al desarrollo, y el por qué, el cómo permanece aún esquivo para muchos visitantes a casa. Este estudio cualitativo usó un bien fundado acercamiento teórico modificado para examinar la manera de facilitar las interacciones cuidador-niño durante las visitas a casa. Con base en la calidad del proceso facilitador, se seleccionaron grabaciones en video de visitas a casa previamente codificadas usando la Escala de Puntuación de Visitas a Casa (HOVRS) A+. Dieciséis visitas a casa (8 con puntajes del proceso facilitador de más baja calidad y 8 de más alta calidad) se examinaron en cuanto a las diferencias en el comportamiento del visitador a casa que apoya las interacciones progenitor-niño durante las visitas. Identificamos comportamientos contextuales y específicos que los visitadores a casa usaron para hacer que los cuidadores y los niños participaran juntos activamente en las interacciones de apoyo al desarrollo. Los resultados revelaron 11 comportamientos del visitador a casa en que se comprometía la más alta calidad del proceso facilitador. Se categorizaron estos comportamientos en cuatro dominios: condiciones ambientales, ejecución de la actividad, enfoque con base en los puntos fuertes, así como las respuestas del cuidador. Este estudio cualitativo resalta comportamientos específicos y áreas de desarrollo profesional para apoyar servicios de visita a casa más eficaces para un diverso grupo de población de familias con niños pequeños.

La facilitation des interactions parent-enfant est une composante clé des programmes de visite à domicile de le petite enfance, fondés sur des données probantes. Plusieurs mesures d'observation ont été créées afin de capturer efficacement les activités de visite à domicile du point de vue de l'opération. Le domaine a une compréhension raisonnable de ce que les visiteurs à domicile peuvent faire pour facilité des interactions qui soutiennent le développement mais le pourquoi et le comment demeurent insaisissables pour bien des visiteurs à domicile. Cette étude qualitative a utilisé une approche théorique fondée modifiée pour faciliter les interactions personne prenant soin de l'enfant - enfant durant les visites à domicile. Des enregistrements vidéo de visite à domicile, préalablement codés en utilisant l'Echelle d'Evaluation de Visite à Domicile (HOVRS en anglais), A+ ont été sélectionnées basé sur la qualité de la facilitation. Seize visites à domicile (8 de scores de plus faible qualité et 8 de scores de la meilleure qualité de facilitation) ont été examinées pour leurs différences dans les comportements du visiteur à domicile qui soutiennent les interactions parent-enfant durant ces visites. Nous avons identifié des comportements contextuels et spécifiques que les visiteurs à domicile ont utilisés afin d'engager activement les personnes prenant soin des enfants et les enfants ensemble dans des interactions soutenant le développement. Les résultats ont révélé 11 comportements de visiteur à domicile qui dans la facilitation distinguant la facilitation de haute qualité. Ces comportements ont été catégorisés en quatre domaines: conditions environnementales, exécuter l'activité, attention mise sur les forces, et réponses de la personne prenant soin de l'enfant. Cette étude qualitative met en lumière des comportements spécifiques et des domaines de développement professionnel pour soutenir des services efficaces de visite à domicile pour une population diverse de familles avec de jeunes enfants.