RESUMEN
BACKGROUND: Gaucher disease is classified into neuronopathic and non-neuronopathic forms with wide phenotypic variation among patients sharing the same genotype. While homozygosity for the common L444P allele usually correlates with the neuronopathic forms, how a defined genotype leads to a phenotype remains unknown. METHODS: The genetic and epigenetic factors causing phenotypic differences were approached by a clinical association study in 32 children homozygous for the point mutation L444P. Direct sequencing and Southern blots were utilised to establish the genotype and exclude recombinant alleles. Glucocerebrosidase activity was measured in lymphoblast and fibroblast cell lines. RESULTS: Residual enzyme activity was highly variable and did not correlate with the observed clinical course. There was also a wide spectrum of phenotypes. Average age at diagnosis was 15 months, and slowed saccadic eye movements were the most prevalent finding. The most severe systemic complications and highest mortality occurred in splenectomised patients before the advent of enzyme replacement therapy (ERT). On ERT, as morbidity and mortality decreased, developmental and language deficits emerged as a major issue. Some trends related to ethnic background were observed. CONCLUSION: The wide clinical spectrum observed in the L444P homozygotes implicates the contribution of genetic modifiers in defining the phenotype in Gaucher disease.
Asunto(s)
Enfermedad de Gaucher/diagnóstico , Enfermedad de Gaucher/genética , Glucosilceramidasa/genética , Preescolar , Femenino , Genotipo , Glucosilceramidasa/metabolismo , Homocigoto , Humanos , Lactante , Masculino , Fenotipo , Mutación PuntualRESUMEN
Mutations in the gene encoding for the lysosomal enzyme glucocerebrosidase (GBA) result in Gaucher disease. In this study, seven novel missense mutations in the glucocerebrosidase gene (A136E, H162P, K198E, Y205C, F251L, Q350X and I402F) and a splice site mutation (IVS10+2T-->A) were identified by direct sequencing of three amplified segments of the glucocerebrosidase gene. Five of the novel mutations were found in patients with neuronopathic forms of Gaucher disease, two of which, K198E and F251L, appear to be associated with type 2 Gaucher disease.
Asunto(s)
Enfermedad de Gaucher/genética , Glucosilceramidasa/genética , Mutación Missense/genética , Sitios de Empalme de ARN/genética , Alelos , Consanguinidad , Análisis Mutacional de ADN , Etnicidad/genética , Exones/genética , Enfermedad de Gaucher/clasificación , Humanos , Grupos Raciales/genéticaRESUMEN
Adjuvant-induced arthritis in rats is a chronic inflammatory disease, widely used as an animal model for rheumatoid arthritis. In our study the effect of various fractions of dialyzable leukocyte extract (DLE): DLE I-molecular weight below 10 kDa (commercial preparation), DLE II-molecular weight below 5 kDa (suppressor fraction), DLE III-molecular weight 5-10 kDa on rat adjuvant-induced arthritis was studied. The adjuvant arthritic (AA) rats were treated with DLE fractions i.p. in solutions containing an active substance isolated from 12.5 x 10(6) and 6.25 x 10(6) leukocytes from day 1 (adjuvant injected) through day 18, every second day (total 9 times). Various markers of inflammation, immune function and joint destruction were evaluated: hindpaw volume, serum hyaluronic acid, serum albumin and biopterin in urine. All these markers showed a significant improvement after using fraction DLE II in comparison with AA controls. Fractions DLE I and DLE III influenced only some markers of inflammation and immune function. Our results demonstrated a therapeutical effect of fraction DLE II on rat adjuvant-induced arthritis.
Asunto(s)
Artritis Experimental/terapia , Extractos Celulares/uso terapéutico , Leucocitos/química , Animales , Artritis Experimental/metabolismo , Artritis Experimental/patología , Artritis Reumatoide , Biopterinas/orina , Extractos Celulares/aislamiento & purificación , Diálisis , Modelos Animales de Enfermedad , Ácido Hialurónico/sangre , Masculino , Ratas , Ratas Endogámicas Lew , Albúmina Sérica/análisisRESUMEN
The antioxidative activity of stobadine and vinpocetine was studied in vitro by measuring their inhibition effect on the depolymerization of the high-molecular-weight hyaluronan by hydroxyl radicals. The radicals were generated by the Cu(2+)-H2O2 system. Hyaluronan depolymerization was monitored by means of size exclusion chromatography. The antioxidative activity of stobadine and vinpocetine was compared to that of D-mannitol. A 50% inhibition of hyaluronan depolymerization was reached at stobadine and vinpocetine concentrations of 1.7 x 10(-6) and 3.0 x 10(-7) mol l-1, respectively, while a D-mannitol level of 2.6 x 10(-3) mol l-1 was needed to achieve the same inhibitory effect.
Asunto(s)
Antioxidantes/química , Carbolinas/química , Ácido Hialurónico/química , Alcaloides de la Vinca/química , Cromatografía en Gel , Depuradores de Radicales Libres , Manitol/química , Peso MolecularRESUMEN
ConA was immobilized on an epoxy-activated copolymer of 2-hydroxyethyl-methacrylate and ethylene-dimethacrylate and commercially available high-pressure liquid chromatography (HPLC) sorbents Separon HEMA 1000 EL, Separon HEMA 1000 E, and Separon HEMA 1000 EH (Tessek, Prague, CSFR Denmark). Specific, sensitive, and rapid method for determination of immobilized ConA lectin activity was developed. beta-Galactosidase from Aspergilus oryzae oligomannosyl residues was used as specific affinant. After separation of bound and unbound beta-galactosidase, enzyme activity was measured in supernatant and thus immobilized ConA lectin activity was calculated easily. The use of the method for evaluating the properties of immobilized ConA, efficiency of immobilization, specific activity, and thermostability is shown. The method developed could be generalized by using artificially glycosylated enzyme for any lectin.
Asunto(s)
Aspergillus/enzimología , Concanavalina A/análisis , Lectinas/análisis , beta-Galactosidasa/química , Concanavalina A/química , Calor , Lectinas/química , Unión Proteica , Sensibilidad y Especificidad , Factores de TiempoRESUMEN
A simple High performance liquid chromatographic (HPLC) method for the specific determination of the molecular weight and concentration of hyaluronic acid (HA) in complex mixtures has been developed. Hyaluronate-binding proteins isolated from bovine cartilage labelled by 125I or fluoresceinisothiocyanate were used as specific markers. The specific binding affinities of the markers were compared and were found to have association constants of 1.6 x 10(7) M-1 and 1.2 x 10(7) M-1 respectively. The HA levels and molecular weight distributions can be easily determined in the range 10-500 ng/mL in complex mixtures by the use of markers, molecular sieving HPLC columns and appropriate detectors. It has been demonstrated clearly that the method is useful for the highly specific determination of the parameters in complex biological samples such as serum and synovial fluids and is recommended for clinical applications.
Asunto(s)
Líquidos Corporales/química , Proteínas Portadoras/metabolismo , Cromatografía Líquida de Alta Presión/métodos , Ácido Hialurónico/análisis , Animales , Artritis Reumatoide/metabolismo , Cartílago/química , Bovinos , Fluoresceína-5-Isotiocianato , Colorantes Fluorescentes , Receptores de Hialuranos , Ácido Hialurónico/química , Ácido Hialurónico/metabolismo , Radioisótopos de Yodo , Peso Molecular , Líquido Sinovial/químicaRESUMEN
Gaucher disease, the most common lysosomal storage disorder, results from the inherited deficiency of the enzyme glucocerebrosidase. Three clinical types are recognized: type 1, non-neuronopathic; type 2, acute neuronopathic; and type 3, subacute neuronopathic. Type 2 Gaucher disease, the rarest type, is progressive and fatal. We have performed molecular analyses of a cohort of 31 patients with type 2 Gaucher disease. The cases studied included fetuses presenting prenatally with hydrops fetalis, infants with the collodion baby phenotype, and infants diagnosed after several months of life. All 62 mutant glucocerebrosidase (GBA) alleles were identified. Thirty-three different mutant alleles were found, including point mutations, splice junction mutations, deletions, fusion alleles and recombinant alleles. Eleven novel mutations were identified in these patients: R131L, H255Q, R285H, S196P, H311R, c.330delA, V398F, F259L, c.533delC, Y304C and A190E. Mutation L444P was found on 25 patient alleles. Southern blots and direct sequencing demonstrated that mutation L444P occurred alone on 9 alleles, with E326K on one allele and as part of a recombinant allele on 15 alleles. There were no homozygotes for point mutation L444P. The recombinant alleles that included L444P resulted from either reciprocal recombination or gene conversion with the nearby glucocerebrosidase pseudogene, and seven different sites of recombination were identified. Homozygosity for a recombinant allele was associated with early lethality. We have also summarized the literature describing mutations associated with type 2 disease, and list 50 different mutations. This report constitutes the most comprehensive molecular study to date of type 2 Gaucher disease, and it demonstrates that there is significant phenotypic and genotypic heterogeneity among patients with type 2 Gaucher disease. Hum Mutat 15:181-188, 2000. Published 2000 Wiley-Liss, Inc.
Asunto(s)
Enfermedad de Gaucher/enzimología , Enfermedad de Gaucher/genética , Glucosilceramidasa/genética , Mutación/genética , Alelos , Etnicidad/genética , Exones/genética , Enfermedad de Gaucher/clasificación , Enfermedad de Gaucher/embriología , Genes Letales/genética , Heterogeneidad Genética , Humanos , Lactante , Seudogenes/genética , Proteínas Recombinantes de Fusión/genéticaRESUMEN
Methyl-alpha-D-mannopyranoside, mannooligosaccharides obtained by acetolysis of yeast mannan, and pure mannans isolated from the cell walls of pathogenic (Candida albicans) and nonpathogenic (Saccharomyces cerevisiae) yeasts were used for treatment of rat adjuvant arthritis. The arthritis was induced by the application of Freund's complete adjuvant into the tail region of rats. The mannose substances were injected into the arthritic rats intraperitonealy at different time intervals. Levels of serum albumin, changes in hindpaws swelling and radiographs were measured in infected rats as variables of the inflammation and destructive arthritic changes. While mannan from C. albicans inhibited both the inflammation and destructive arthritic changes, mannan from S. cerevisiae showed less effect. However, acetolysate of S. cerevisiae mannan as well as simple methyl-alpha-D-mannopyranoside inhibited both inflammation and destructive arthritic changes to a similar degree as mannan isolated from C. albicans. The effect, which is not dose dependent indicates its possible immunoregulatory mechanism. This is the first time a therapeutic effect of simple carbohydrates on rat adjuvant arthritis has been described.
Asunto(s)
Artritis Experimental/prevención & control , Mananos/farmacología , Metilmanósidos/farmacología , Oligosacáridos/farmacología , Levaduras/metabolismo , Animales , Artritis Experimental/complicaciones , Artritis Experimental/diagnóstico por imagen , Edema/etiología , Pie , Miembro Posterior , Masculino , Mananos/metabolismo , Radiografía , Ratas , Ratas Endogámicas Lew , Albúmina Sérica/análisisRESUMEN
Congenital disorders of glycosylation (CDG) are a group of multisystemic disorders resulting from defects in the synthesis and processing of N-linked oligosaccharides. The most common form, CDG type Ia (CDG-Ia), results from a deficiency of the enzyme phosphomannomutase (PMM). PMM converts mannose 6-phosphate (man-6-P) to mannose-1-phosphate (man-1-P), which is required for the synthesis of GDP-mannose, a substrate for dolichol-linked oligosaccharide synthesis. The traditional assay for PMM, a coupled enzyme system based on the reduction of NADP(+) to NADPH using man-1-P as a substrate, has limitations in accuracy and reproducibility. Therefore, a more sensitive, direct test for PMM activity, based on the detection of the conversion of man-1-P to man-6-P by high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), was developed. Using this assay, the activity of PMM was markedly deficient in fibroblasts and lymphoblasts from 23 patients with CDG-Ia (range 0-15.3% of control, average 4.9+/-4.7%) and also decreased in seven obligate heterozygotes (range 33.0-72.0% of control, average 52.2+/-14.7%). Unlike the spectrophotometric method, there was no overlap in PMM activity among patients, obligate heterozygotes, or controls. Thus, the PMM assay based on HPAEC-PAD has increased utility in the clinical setting, and can be used, together with transferrin isoelectric focusing, to diagnose patients with CDG-Ia and to identify heterozygotes when clinically indicated.
Asunto(s)
Errores Innatos del Metabolismo de los Carbohidratos/metabolismo , Cromatografía por Intercambio Iónico/métodos , Manosafosfatos/metabolismo , Fosfotransferasas (Fosfomutasas)/metabolismo , Aniones , Errores Innatos del Metabolismo de los Carbohidratos/enzimología , Errores Innatos del Metabolismo de los Carbohidratos/genética , Línea Celular , Fibroblastos/citología , Fibroblastos/enzimología , Glicosilación , Heterocigoto , Humanos , Concentración de Iones de Hidrógeno , Linfocitos/citología , Linfocitos/enzimología , Manosafosfatos/análisis , Fosfotransferasas (Fosfomutasas)/deficiencia , Fosfotransferasas (Fosfomutasas)/genética , Sensibilidad y EspecificidadRESUMEN
Gustin, a zinc-metalloprotein constituting about 3% of human parotid saliva protein was previously isolated and characterized as a single polypeptide chain of 37kDa with one mole of zinc tightly bound to the protein. It exhibited biological activity activating calmodulin dependent bovine brain cAMP phosphodiesterase and was decreased in saliva of patients with loss of taste in whom taste buds showed a specific pathological morphology. Determination of its primary structure by amino acid sequence revealed it was identical with carbonic anhydrase (CA) [EC 4.2.1.1] VI and had two N-linked glycosylation sites. Analysis by reverse phase HPLC and SDS-PAGE before and after deglycosylation confirmed a single peak with molecular weight of the purified protein being 37kDa, the deglycosylated protein, 33kDa. N-linked carbohydrate chains contained N-acetyl glucosamine, galactose, mannose, and fucose interior to di, tri and tetra sialyated termini. By isoelectric focusing five increasingly acidic pI values were determined consistent with addition of sialic acid as the terminal carbohydrate residue on the N-linked glycoforms of the protein. Gustin was found to exhibit CA activity but was inhibited by known CA inhibitors in a different manner than CA I or II. These findings, consistent with analysis of previous investigators, indicate that parotid saliva gustin is CA VI.
Asunto(s)
Anhidrasas Carbónicas/genética , Glándula Parótida/metabolismo , Saliva/metabolismo , Proteínas y Péptidos Salivales/genética , Secuencia de Aminoácidos , Animales , Anhidrasas Carbónicas/química , Anhidrasas Carbónicas/metabolismo , Bovinos , Cromatografía Líquida de Alta Presión , ADN Complementario/análisis , ADN Complementario/genética , Humanos , Datos de Secuencia Molecular , Proteínas y Péptidos Salivales/química , Proteínas y Péptidos Salivales/metabolismo , Alineación de Secuencia , Análisis de SecuenciaRESUMEN
Among the many phenotypes associated with Gaucher disease, the inherited deficiency of glucocerebrosidase, are reports of patients with parkinsonian symptoms. The basis for this association is unknown, but could be due to alterations in the gene or gene region. The human glucocerebrosidase gene, located on chromosome 1q21, has a nearby pseudogene that shares 96% identity. Immediately adjacent to the glucocerebrosidase pseudogene is a convergently transcribed gene, metaxin, which has a pseudogene that is located just downstream to the glucocerebrosidase gene. We describe a patient with mild Gaucher disease but impaired horizontal saccadic eye movements who developed a tremor at age 42, followed by rapid deterioration of her gait. A pallidotomy at age 47 was unsuccessful. Her motor and cognitive deterioration progressed despite enzyme replacement therapy. Sequencing of the glucocerebrosidase gene identified mutations L444P and D409H. Southern blot analysis using the enzyme SspI showed that the maternal allele had an additional 17-kb band. PCR amplifications and sequencing of this fragment demonstrated a duplication which included the glucocerebrosidase pseudogene, metaxin gene, and a pseudometaxin/metaxin fusion. Gene alterations associated with this novel rearrangement, resulting from a crossover between the gene for metaxin and its pseudogene, could contribute to the atypical phenotype encountered in this patient.
Asunto(s)
Enfermedad de Gaucher/genética , Enfermedad de Gaucher/fisiopatología , Glucosilceramidasa/genética , Enfermedad de Parkinson Secundaria/complicaciones , Enfermedad de Parkinson Secundaria/genética , Ubiquitina-Proteína Ligasas , Alelos , Southern Blotting , Cromosomas Humanos Par 1/genética , Intercambio Genético/genética , Análisis Mutacional de ADN , Femenino , Enfermedad de Gaucher/enzimología , Enfermedad de Gaucher/terapia , Genotipo , Glucosilceramidasa/deficiencia , Glucosilceramidasa/metabolismo , Glucosilceramidasa/uso terapéutico , Humanos , Hibridación Fluorescente in Situ , Ligasas/genética , Masculino , Persona de Mediana Edad , Proteínas de Transporte de Membrana Mitocondrial , Proteínas del Tejido Nervioso/genética , Enfermedad de Parkinson Secundaria/enzimología , Enfermedad de Parkinson Secundaria/fisiopatología , Fenotipo , Mapeo Físico de Cromosoma , Polimorfismo Genético/genética , Proteínas/genética , Seudogenes/genética , ARN Mensajero/análisis , ARN Mensajero/genética , SinucleínasRESUMEN
Gaucher disease, the most common of the sphingolipidoses, results from the inherited deficiency of the enzyme glucocerebrosidase (EC 3.2.1.45). Although type 2 (acute neuronopathic) Gaucher disease is associated with rapidly progressive and fatal neurologic deterioration, the pathophysiologic mechanisms leading to the neurologic symptoms and early demise remain uncharacterized. While the pathology encountered in Gaucher disease has been attributed to glucocerebroside storage, glucosylsphingosine (Glc-sph), a cytotoxic compound, also accumulates in the tissues. Elevations of brain Glc-sph have been reported in patients with types 2 and 3 Gaucher disease. In this study, Glc-sph levels were measured using HPLC in tissues from mice with type 2 Gaucher disease created with a null glucocerebrosidase allele. Compared with unaffected littermates, homozygous mice with type 2 Gaucher disease had approximately a 100-fold elevation of Glc-sph in brain, as well as elevated levels in other tissues. This accumulation was detected in utero by E 13 and increased progressively throughout gestation. Similarly, elevated Glc-sph levels were seen in human fetuses with type 2 Gaucher disease, indicating that therapy initiated after birth may be too late to prevent the sequelae of progressive neurologic damage that begins early in gestation. These findings suggest that the accumulation of Glc-sph may be responsible for the rapid demise of mice with type 2 Gaucher disease and the devastating clinical course seen in patients with type 2 Gaucher disease.
Asunto(s)
Desarrollo Embrionario y Fetal , Enfermedad de Gaucher/embriología , Enfermedad de Gaucher/metabolismo , Glucosilceramidasa/genética , Esfingosina/análogos & derivados , Animales , Cromatografía Líquida de Alta Presión , Enfermedad de Gaucher/fisiopatología , Edad Gestacional , Heterocigoto , Humanos , Hidropesía Fetal/patología , Ratones , Ratones Mutantes , Reacción en Cadena de la Polimerasa , Psicosina/análogos & derivados , Esfingosina/metabolismoRESUMEN
Among the phenotypes associated with Gaucher disease, the deficiency of glucocerebrosidase, are rare patients with early onset, treatment-refractory parkinsonism. Sequencing of glucocerebrosidase in 17 such patients revealed 12 different genotypes. Fourteen patients had the common "non-neuronopathic" N370S mutation, including five N370S homozygotes. While brain glucosylsphingosine levels were not elevated, Lewy bodies were seen in the four brains available for study. The shared clinical and neuropathologic findings in this subgroup suggest that the deficiency in glucocerebrosidase may contribute to a vulnerability to parkinsonism.
Asunto(s)
Enfermedad de Gaucher/genética , Predisposición Genética a la Enfermedad , Glucosilceramidasa/deficiencia , Glucosilceramidasa/genética , Enfermedad de Parkinson/etiología , Esfingosina/análogos & derivados , Adulto , Southern Blotting , Encéfalo/metabolismo , Encéfalo/patología , Desoxirribonucleasas de Localización Especificada Tipo II/genética , Femenino , Enfermedad de Gaucher/complicaciones , Enfermedad de Gaucher/metabolismo , Enfermedad de Gaucher/patología , Homocigoto , Humanos , Levodopa/uso terapéutico , Masculino , Persona de Mediana Edad , Mutación , Enfermedad de Parkinson/tratamiento farmacológico , Enfermedad de Parkinson/metabolismo , Enfermedad de Parkinson/patología , Psicosina/análogos & derivados , Esfingosina/metabolismoRESUMEN
"French type" sialuria, a presumably dominant disorder that, until now, had been documented in only five patients, manifests with mildly coarse facies, slight motor delay, and urinary excretion of large quantities (>1 g/d) of free N-acetylneuraminic acid (NeuAc). The basic defect consists of the very rare occurrence of failed feedback inhibition of a rate-limiting enzyme, in this case uridinediphosphate-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase, by a downstream product, in this case cytidine monophosphate (CMP)-NeuAc. We report a new patient with sialuria who has a heterozygous G-->A substitution in nucleotide 848 of the epimerase gene, which results in an R266Q change. The proband's other allele, as expected, had no mutation. However, the heterozygous R266Q mutation was detected in the patient's mother, who has similarly increased urinary levels of free NeuAc, thereby confirming, for the first time, the dominant mode of inheritance of this inborn error. The biochemical diagnosis of the proband was verified by the greatly increased level of free NeuAc in his cultured fibroblasts, the NeuAc distribution, mainly (59%) in the cytoplasm, and by the complete failure of 100 microM CMP-NeuAc to inhibit UDP-GlcNAc 2-epimerase activity in the mutant cells. These findings call for expansion of the phenotype to include adults and for more-extensive assaying of free NeuAc in the urine of children with mild developmental delay. The prevalence of sialuria is probably grossly underestimated.