Enhanced azadirachtin production in neem (Azadirachta indica) callus through NaCl elicitation: Insights into differential protein regulation via shotgun proteomics.

With their remarkable bioactivity and evolving commercial importance, plant secondary metabolites (PSMs) have gained significant research interest in recent years. Plant tissue culture serves as a credible tool to examine how abiotic stresses modulate the production of PSMs, enabling clear insights into plant stress responses and the prospects for controlled synthesis of bioactive compounds. Azadirachta indica, or neem has been recognized as a repository of secondary metabolites for centuries, particularly for the compound named azadirachtin, due to its bio-pesticidal and high antioxidant properties. Introducing salt stress as an elicitor makes it possible to enhance the synthesis of secondary metabolites, specifically azadirachtin. Thus, in this research, in vitro callus cultures of neem were micro-propagated and induced with salinity stress to explore their effects on the production of azadirachtin and identify potential proteins associated with salinity stress through comparative shotgun proteomics (LCMS/MS). To induce salinity stress, 2-month-old calli were subjected to various concentrations of NaCl (0.05-1.5%) for 4 weeks. The results showed that the callus cultures were able to adapt and survive in the salinity treatments, but displayed a reduction in fresh weight as the NaCl concentration increased. Notably, azadirachtin production was significantly enhanced in the salinity treatment compared to control, where 1.5% NaCl-treated calli produced the highest azadirachtin amount (10.847 ± 0.037 mg/g DW). The proteomics analysis showed that key proteins related to primary metabolism, such as defence, energy, cell structure, redox, transcriptional and photosynthesis, were predominantly differentially regulated (36 upregulated and 93 downregulated). While a few proteins were identified as being regulated in secondary metabolism, they were not directly involved in the synthesis of azadirachtin. In conjunction with azadirachtin elicitation, salinity stress treatment could therefore be successfully applied in commercial settings for the controlled synthesis of azadirachtin and other plant-based compounds. Further complementary omics approaches can be employed to enhance molecular-level modifications, to facilitate large-scale production of bioactive compounds in the future.

Fetal Glucocorticoid Mediates the Association between Prenatal Per- and Polyfluoroalkyl Substance Exposure and Neonatal Growth Index: Evidence from a Birth Cohort Study.

Glucocorticoid plays a key role in the growth and organ maturation of fetus. However, the effect of glucocorticoid on the association between per- and polyfluoroalkyl substance (PFAS) exposure and fetal growth is still unknown. We detected cord cortisol (active glucocorticoid in human) and 34 PFAS concentrations in the maternal serum samples, which were collected from 202 mother-fetus pairs in the Maoming Birth Cohort from 2015 to 2018. The mediation effect of cord cortisol on the association between maternal PFAS and the neonatal growth index (NGI) was estimated. We found that higher PFAS concentrations were associated with lower NGI in terms of ponderal index, birth weight (BW), head circumference (HC), and its z-scores (BWZ and HCZ) (P < 0.05). Fetal cortisol could mediate 12.6-27.3% of the associations between PFAS and NGI. Specifically, cord cortisol mediated the association between branched perfluorooctane sulfonate (branched PFOS) and HCZ by 20.4% and between perfluorooctanoate (PFOA) and HCZ by 27.3%. Our findings provide the first epidemiological data evincing that fetal cortisol could mediate the association between prenatal PFAS exposure and fetal growth. Further investigations are recommended to elucidate the interactions among cord cortisol, PFAS, and fetal growth.

Development of a time-resolved fluoroimmunoassay for salmonid insulin-like growth factor binding protein-2b.

Insulin-like growth factor (IGF)-1 promotes the growth of vertebrates, and its binding proteins (IGFBPs) regulate the activity of circulating IGF-1. Three IGFBPs, IGFBP-2b, -1a, and -1b, were consistently detected in the circulatory system of salmonids. IGFBP-2b is thought to be the main carrier of IGFs and promoter of IGF-1-mediated growth in salmonids. Currently, there are no immunoassays for detecting IGFBP-2b. In this study, we developed a time-resolved fluoroimmunoassay (TR-FIA) for IGFBP-2b detection in salmonid fishes. To establish TR-FIA, we produced two recombinant trout (rt) IGFBP-2bs expressed, one with thioredoxin (Trx) and a histidine (His) tag, and the other with His-tag only. We labeled both recombinant proteins with europium (Eu). Only Eu-Trx.His.rtIGFBP-2b cross-reacted with anti-IGFBP-2b, and the addition of increasing amounts of Trx.His.rtIGFBP-2b replaced the binding, indicating its utility as a tracer and assay standard. The addition of unlabeled salmon IGF-1 did not affect the binding of the standard or sample. Serial dilution curves of sera from rainbow trout, Chinook salmon, and chum salmon were parallel to those of the standard. The assay range (ED80-ED20) of the TR-FIA was 60.4 to 251.3 ng/ml, and its minimum detection limit of this assay was 21 ng/ml. The intra- and inter-assay coefficients of variation were 5.68% and 5.65%, respectively. Circulating IGFBP-2b levels in fed rainbow trout were higher than those in fasted fish and were correlated with individual growth rates. This TR-FIA is useful for further exploring the physiological responses of circulating IGFBP-2b and evaluating the growth status of salmonids.

Diverging or converging to a green world? Impact of green growth measures on countries' economic performance.

Green growth has emerged in recent years to respond to environmental problems caused by climate change and the scarcity of resources. However, today's green growth involves environmental, social and financial dimensions. In this context, many countries are currently seeking green growth for their economic development through the efficient use of their resources. This study aims to assess the impact of green growth performance on the economic development of countries. A quantitative approach was applied to a sample of 172 countries worldwide, and the formulated hypotheses were tested through multiple linear regressions estimated by the ordinary least squares method. The economic development of countries was measured by the Human Development Index (HDI) and measures the sustainability performance of countries by the Green Growth Index (GGI). The results of this study demonstrate that (i) the measures of green growth performance have a positive impact on the economic development of high-income, upper-middle-income, and lower-middle-income economies, (ii) in poorer economies, less is the contribution of green growth to economic development, mainly because they neglecting the social dimension despite the numerous existing projects in these economies for greater inclusion and (iii) green economic opportunities (green investment, green trade, green employment and green innovation) do not influence green economic development in all analysed economies. Consequently, suggestions were made for policymakers from different groups of countries to increase and accelerate their sustainable green growth. Literature on economic development and green growth is still scarce, especially at the empirical level, and few studies use the 2020 GGI data. In addition, this study also uses recent rankings of world economies to categorize the economic development of countries.

Estimation of Greenhouse Lettuce Growth Indices Based on a Two-Stage CNN Using RGB-D Images.

Growth indices can quantify crop productivity and establish optimal environmental, nutritional, and irrigation control strategies. A convolutional neural network (CNN)-based model is presented for estimating various growth indices (i.e., fresh weight, dry weight, height, leaf area, and diameter) of four varieties of greenhouse lettuce using red, green, blue, and depth (RGB-D) data obtained using a stereo camera. Data from an online autonomous greenhouse challenge (Wageningen University, June 2021) were employed in this study. The data were collected using an Intel RealSense D415 camera. The developed model has a two-stage CNN architecture based on ResNet50V2 layers. The developed model provided coefficients of determination from 0.88 to 0.95, with normalized root mean square errors of 6.09%, 6.30%, 7.65%, 7.92%, and 5.62% for fresh weight, dry weight, height, diameter, and leaf area, respectively, on unknown lettuce images. Using red, green, blue (RGB) and depth data employed in the CNN improved the determination accuracy for all five lettuce growth indices due to the ability of the stereo camera to extract height information on lettuce. The average time for processing each lettuce image using the developed CNN model run on a Jetson SUB mini-PC with a Jetson Xavier NX was 0.83 s, indicating the potential for the model in fast real-time sensing of lettuce growth indices.

Viability assessment for the use of floating treatment wetlands as alternative production and remediation systems for nursery and greenhouse operations.

Floating treatment wetlands (FTWs) are deployed in nursery and greenhouse water retention reservoirs to help manage and mitigate nutrient contaminants within irrigation return flow. One management issue for FTWs is the need for plant harvest to avoid releasing nutrients back into the water column when plant tissues begin to senesce. Some researchers recommend harvesting the entire plant to prevent nutrient release. Resale of plants harvested from FTWs is one possible use of this live biomass by the nursery and greenhouse industry. To determine if nursery and greenhouse operations could use FTWs to clean water and simultaneously produce saleable plants, the objectives of this study were to (1) quantify the survival and aesthetics of plants after transplant from a FTW and (2) compare the time to market between traditional (containerized) and FTW production systems. Overall, plants harvested from FTWs were resalable. Plants transplanted from FTWs had higher survival and aesthetic ratings (scale of 1-5, 5 = highest quality) when transplanted to containers (4.26) compared to directly into the ground (2.81). The growth of Panicum virgatum and Canna × generalis 'Firebird' in FTW systems was two weeks slower than that of plants grown in traditional production areas. Sustainability of FTW systems combined with the capacity to produce salable plants that will return a profit improves adoption feasibility for production horticulture.

A tale of two metrics: the EPA Risk Quotient Approach versus the delay in Population Growth Index for determination of pesticide risk to aquatic species.

The potential risk that two closely related insecticides, spinetoram and spinosad, posed to three Cladoceran species, Ceriodaphnia dubia, Daphnia pulex, and D. magna was determined using two approaches, the USEPA Risk Quotient method and the Delay in Population Growth Index (DPGI). Results of the RQ method showed that spinetoram posed a risk to all three species, but spinosad posed a risk only to C. dubia. The DPGI analysis showed that exposure to spinetoram resulted in populations of all three species being delayed ≥ 3 generation times. Exposure to the LC50 and the lower 95% CL resulted in delayed populations while exposure to the upper 95% CL concentration of spinetoram resulted in no recovery of any of the three species over the course of the modeling exercise (88 d). Exposure to the lower and upper 95% Cl and the LC50 of spinosad resulted in C. dubia populations being delayed ≥ 3 generations. D. pulex populations were not negatively affected after exposure to spinosad. D. magna populations were delayed ≥ 3 generations, but only after exposure to the upper 95% Cl of spinosad. These results illustrate that although the EPA risk quotient method indicated that spinetoram posed a risk to all three species and that spinosad only posed a risk to C. dubia, the DPGI showed that D. magna would be negatively affected by spinosad and none of the three species would reach a predetermined number of individuals after exposure to the upper 95% CL of spinetoram. Because the DPGI uses the 95% Cl as well as the LC50 in its calculation and produces a measure of population growth it provides more detailed information in terms of the potential risk of pesticides to populations than the RQ method.

Effect of different carbon sources on the growth and enzyme production of a toxigenic and a non-toxigenic strain of Aspergillus flavus.

Two strains of A. flavus one toxigenic (CECT 2687) and the other non-toxigenic (NRRL 6541) were studied for their genomic potential, growth capacity, and the production of enzymes on simple sugars, polysaccharides, and complex substrates under solid-state fermentation (SSF). According to the genome analysis, this fungus has many genes to degrade different types of polysaccharides and therefore it would be able to grow on different substrates. Both strains grow in all the carbon sources, but visibly CECT2687 grows slower than NRRL6541. However, we propose the growth index (GI) to establish a dry weight-diameter relationship as a more reliable measure that truly shows the growth preferences of the fungus. Considering this, the NRRL6541 shows less growth in 11 of the 16 evaluated carbon sources than CECT2687. Complex substrates were the best carbon source for the growth of both strains. Corncob (CC) induced the production of xylanases, pectinases, and almost all the accessory enzymes evaluated (except for α-xylosidase) this could make it an agricultural waste of interest to produce hemicellulolytic enzymes. Both strains produce a great variety of xylanases and pectinases (pathogenicity factors) making A. flavus a good potential candidate for the degradation of polysaccharides with a high content of xylan and pectin.

A spatially explicit empirical model of structural development processes in natural forests based on climate and topography.

Stand structure develops with stand age. Old-growth forests with well-developed stand structure support many species. However, development rates of stand structure likely vary with climate and topography. We modeled structural development of 4 key stand variables and a composite old-growth index as functions of climatic and topographic covariates. We used a hierarchical Bayesian method for analysis of extensive snap-shot National Forest Inventory (NFI) data in Japan (n = 9244) to account for differences in stand age. Development rates of structural variables and the old-growth index exhibited curvilinear responses to environmental covariates. Flat sites were characterized by high rates of structural development. Approximately 150 years were generally required to attain high values (approximately 0.8) of the old-growth index. However, the predicted age to achieve specific values varied depending on environmental conditions. Spatial predictions highlighted regional variation in potential structural development rates. For example, sometimes there were differences of >100 years among sites, even in the same catchment, in attainment of a medium index value (0.5) after timber harvesting. The NFI data suggested that natural forests, especially old natural forests (>150 years), remain generally on unproductive ridges, steep slopes, or areas with low temperature and deep snow, where many structural variables show slow development rates. We suggest that maintenance and restoration of old natural forests on flat sites should be prioritized for conservation due to the likely rapid development of stand structure, although remaining natural forests on low-productivity sites are still important and should be protected.

Un Modelo Empírico Espacialmente Explícito de Procesos de Desarrollo Estructural en Bosques Naturales Basado en el Clima y la Topografía Resumen La estructura de un rodal se desarrolla con la edad. Los bosques maduros con una estructura bien desarrollada dan sustento a muchas especies. Sin embargo, las tasas de desarrollo de los rodales probablemente varían con el clima y la topografía. Modelamos el desarrollo estructural de 4 variables clave de un rodal y un índice de crecimiento antiguo compuesto como funciones de covariables climáticas y topográficas. Utilizamos un método bayesiano jerárquico para analizar datos (n = 9,244) en fotografías del inventario nacional forestal (INF) de Japón para entender las diferencias en la edad de los rodales. Las tasas de desarrollo de las variables estructurales y el índice de crecimiento antiguo mostraron respuestas curvilíneas a las covariables ambientales. Los sitios planos se caracterizaron por altas tasas de desarrollo estructural. Por lo general, se requerían 150 años para alcanzar valores altos (∼0.8) en el índice de crecimiento antiguo. Sin embargo, la edad prevista para alcanzar valores específicos varió dependiendo de las condiciones ambientales. Las predicciones espaciales pusieron de relieve la variación regional en las potenciales tasas de desarrollo estructural. Por ejemplo, a veces había diferencia de >100 años entre sitios, aun en la misma cuenca, en el consecución de un valor de índice medio (0.5) después de la cosecha de madera. Los datos de INF sugieren que los bosques naturales, especialmente los bosques maduros (>150 años) permanecen generalmente en crestas improductivas, pendientes pronunciadas o en áreas con baja temperatura y nieve profunda, donde muchas variables estructurales muestran tasas de desarrollo lentas. Sugerimos que el mantenimiento y la restauración de bosques naturales maduros en sitios planos deben priorizarse para conservación debido al probable desarrollo rápido de la estructura del rodal, aunque los bosques naturales restantes en sitios de baja productividad también son importantes y deben ser protegidos.

Development of an Apparatus for Crop-Growth Monitoring and Diagnosis.

To non-destructively acquire leaf nitrogen content (LNC), leaf nitrogen accumulation (LNA), leaf area index (LAI), and leaf dry weight (LDW) data at high speed and low cost, a portable apparatus for crop-growth monitoring and diagnosis (CGMD) was developed according to the spectral monitoring mechanisms of crop growth. According to the canopy characteristics of crops and actual requirements of field operation environments, splitting light beams by using an optical filter and proper structural parameters were determined for the sensors. Meanwhile, an integral-type weak optoelectronic signal processing circuit was designed, which changed the gain of the system and guaranteed the high resolution of the apparatus by automatically adjusting the integration period based on the irradiance received from ambient light. In addition, a coupling processor system for a sensor information and growth model based on the microcontroller chip was developed. Field experiments showed that normalised vegetation index (NDVI) measured separately through the CGMD apparatus and the ASD spectrometer showed a good linear correlation. For measurements of canopy reflectance spectra of rice and wheat, their linear determination coefficients (R²) were 0.95 and 0.92, respectively while the root mean square errors (RMSEs) were 0.02 and 0.03, respectively. NDVI value measured by using the CGMD apparatus and growth indices of rice and wheat exhibited a linear relationship. For the monitoring models for LNC, LNA, LAI, and LDW of rice based on linear fitting of NDVI, R² were 0.64, 0.67, 0.63 and 0.70, and RMSEs were 0.31, 2.29, 1.15 and 0.05, respectively. In addition, R² of the models for monitoring LNC, LNA, LAI, and LDW of wheat on the basis of linear fitting of NDVI were 0.82, 0.71, 0.72 and 0.70, and RMSEs were 0.26, 2.30, 1.43, and 0.05, respectively.

Improved accumulation of betulin and betulinic acid in cell suspension culture of Betula pendula roth by abiotic and biotic elicitors.

Betulin (B) and betulinic acid (BA) are two triterpenes with diverse pharmacological and physiological actions. Elicitation of Betula pendula Roth cell cultures by elicitors is an excellent strategy to increase B and BA levels. Six abiotic and biotic elicitors were studied to improve accumulation of B and BA in the cell culture of B. pendula. The B and BA production in treated cells was verified by HPLC. The results showed the maximum growth index (7) on day 3 in cells treated with 0.5 mg L-1 chlorocholine chloride (CCC). The increased accumulation of BA in the cells treated with 200 mg L-1 of chitosan was found to be 5.9 × (6.5 mg g-1 DW) higher over control cells. Treating the cells with 2 mg L-1 of CCC, after 7 days, led to 149.3× enhancement of B content (19.4 mg g-1 DW) over the controls. Production of this triterpenoid at a much shorter time with a much higher growth rate can be economic and lead to producing large amounts of B and BA for anti-cancer and HIV drugs preparation.

Autochthonous lactic acid bacteria with probiotic aptitudes as starter cultures for fish-based products.

This study focused on the selection of lactic starters with probiotic properties for the production of fermented fish-products by the use of a multivariate approach (Cluster Analysis and Principal Component Analysis). Seventy-five isolates were recovered from fish intestinal microbiota and characterized by evaluating phenotypical, technological and probiotic traits; the most promising isolates were molecularly identified and then used into fish fermented sausage production. Namely, data from technological characterization were modelled through Growth Index and used as input to run a preliminary selection. Thus, 15 promising strains were selected and subjected to probiotic characterization; considering the results from probiotic tests, 3 promising strains were finally chosen (11, 68 and 69), identified as members of the genus Lactobacillus and used for the validation at laboratory level through the assessment of their performances for the production of fermented fish sausages. The results were promising as the use of the selected strains reduced the fermentation time (2 days) ensuring a good microbiological quality of the final product.

Effects of seawater transfer and fasting on the endocrine and biochemical growth indices in juvenile chum salmon (Oncorhynchus keta).

Insulin-like growth factor (IGF)-I, IGF-binding protein (IGFBP)-1 and RNA/DNA ratio are endocrine and biochemical parameters used as growth indices in fish, however, they are subjected to environmental modulation. Chum salmon (Oncorhynchus keta) migrate from freshwater (FW) to seawater (SW) at fry/juvenile stage weighing around 1g and suffer growth-dependent mortality during the early phase of their marine life. In order to reveal environmental modulation of the IGF/IGFBP system and establish a reliable growth index for juvenile chum salmon, we examined effects of SW transfer and fasting on IGF-I, IGFBP-1 and RNA/DNA ratio, and correlated them to individual growth rate. Among serum IGF-I, liver and muscle igf-1, igfbp-1a, igfbp-1b and RNA/DNA ratio examined, muscle RNA/DNA ratio and muscle igfbp-1a responded to SW transfer. Serum IGF-I, liver igf-1 and liver RNA/DNA ratio were sensitive to nutritional change by being reduced in 1week in both FW and SW while muscle igf-1 was reduced 2weeks after fasting. In contrast, igfbp-1a in both tissues was increased by 2weeks of fasting and igfbp-1b in the liver of SW fish was increased in 1week. These results suggest that the sensitivity of igf-1 and igfbp-1s to fasting differs between tissues and subtypes, respectively. When chum salmon juveniles in SW were marked and subjected to feeding or fasting, serum IGF-I showed the highest correlation with individual growth rate. Liver igfbp-1a and -1b, and muscle igf-1 exhibited moderate correlation coefficients with growth rate. These results show that serum IGF-I is superior to the other parameters as a growth index in juvenile chum salmon in term of its stability to salinity change, high sensitivity to fasting and strong relationship with growth rate. On the one hand, when collecting blood from chum salmon fry/juveniles is not practical, measuring liver igfbp-1a and -1b, or/and muscle igf-1 is an alternative.

The influence of a 21 kDa Kunitz-type trypsin inhibitor from nonhost madras thorn, Pithecellobium dulce, seeds on H. armigera (Hübner) (Lepidoptera: Noctuidae).

A trypsin inhibitor purified from the seeds of the Manila tamarind, Pithecellobium dulce (PDTI), was studied for its effects on growth parameters and developmental stages of Helicoverpa armigera. PDTI exhibited inhibitory activity against bovine trypsin (∼86%; ∼1.33 ug/ml IC50). The inhibitory activity of PDTI was unaltered over a wide range of temperature, pH, and in the presence of dithiothreitol. Larval midgut proteases were unable to digest PDTI for up to 12 h of incubation. Dixon and Lineweaver-Burk double reciprocal plots analysis revealed a competitive inhibition mechanism and a Ki of ∼3.9 × 10(-8) M. Lethal dose (0.50% w/w) and dosage for weight reduction by 50% (0.25% w/w) were determined. PDTI showed a dose-dependent effect on mean larval weight and a series of nutritional disturbances. In artificial diet at 0.25% w/w PDTI, the efficiency of conversion of ingested food, of digested food, relative growth rate, and growth index declined, whereas approximate digestibility, relative consumption rate, metabolic cost, consumption index, and total developmental period were increased in larvae. This is the first report of antifeedant and antimetabolic activities of PDTI on midgut proteases of H. armigera.

Selection of autochthonous strains as promising starter cultures for Fior di Latte, a traditional cheese of southern Italy.

BACKGROUND: This paper describes the selection of promising strains intended as starter cultures from the autochthonous lactic acid bacteria of Fior di Latte cheese (Apulia region, southern Italy). RESULTS: Ninety-five isolates were randomly selected from whey and Fior di Latte. After preliminary characterization based upon Gram staining, deamination of arginine, hydrolysis of esculine and production of CO2 from glucose and citrate, the isolates were studied for their growth at different temperatures (10, 15 and 45 °C), with salt addition (20, 40 and 65 g L(-1) ) and at pH 4.4 and their acidification score in MRS broth after 6 and 24 h. Data were modeled through the growth index and used as input to run a preliminary cluster analysis and a principal component analysis. In this way, nine promising strains were selected and used for validation at laboratory level, to study the acidification score in milk and propose some possible microbial mixtures. CONCLUSION: This paper reports the first research focusing on the design of a lactic starter for the production of Fior di Latte cheese, using a quantitative approach based on the evaluation of growth index and acidification score as well as on the use of a multivariate approach to select the most promising nine strains.

Effect of Taekwondo Training on Physical Fitness and Growth Index According to IGF-1 Gene Polymorphism in Children.

This study analyzed the effect of regular Taekwondo training for 16 weeks on physical fitness and growth index depending on different IGF-1 gene polymorphisms. The subjects of the study were 44 male students who were 8 year years old. The IGF-1 gene showed the highest frequency of 18 CA repeat (190 bp) in 50% of subjects, and was found in the homozygote (n=11), heterozygote (n=22) and non-carriers (n=11). The results of the physical fitness and growth index among the gene polymorphism groups indicated no significant differences but the expected height of the non-carrier group was significantly high (p<0.05). After Taekwondo training, the homozygote group and the non-carrier groups demonstrated significant (p<0.05) increase in grip strength and in time in the standing with one leg while closing eyes test, respectively. Only the homozygote group had a significant (p< 0.05) increase in thigh circumference. IGF-1 concentration significantly (p<0.05) increased in the heterozygote group, while HOMA-IR significantly (p<0.05) decreased in the homozygote group. Furthermore, there was a significant (p<0.05) decrease in glucose in both the homozygote and the non-carriers groups. The difference between physical fitness and growth index depending on the IGF-1 gene polymorphism after Taekwondo training did not show consistent impact.

Selection of promising lactic acid bacteria as starter cultures for sourdough: using a step-by-step approach through quantitative analyses and statistics.

BACKGROUND: The main goal of this research was to show how to use a qualitative assessment of some technological properties of lactic acid bacteria (LAB), combined with the evaluation of the growth index (GI), to select promising starter cultures for sourdough. RESULTS: Fifty-four strains of LAB were isolated from a single factory, identified by molecular tools and studied for their growth as a function of NaCl (20, 40 and 65 g L(-1)), temperature (45, 15 and 10 °C), pH 9.2 and acidification in MRS broth. The growth was evaluated through absorbance and data were modelled as GI. GIs were used to build frequency histograms and to run a principal component analysis (PCA). In this way, six strains, identified as Lactobacillus plantarum and able to grow in a wide range of conditions (temperature, pH and salt) and/or able to decrease the pH by 1.77-2.0 units, were selected and tested in a model system (flour and water) to study the acidification after 24 h and their viability after 14 days. CONCLUSION: The main result of this paper was to show how a simple step-by-step approach could be a useful tool to select promising starter cultures for sourdough. The method was based on (1) strain identification, (2) assessment of some traits through the GI, combined with simple statistical approaches (frequency histograms and PCA), and (3) preliminary validation in model systems.

Reconstruction of ENSO variability using the standardized growth index of a Tridacna shell from Yongshu Reef, South China Sea.

El Niño-Southern Oscillation (ENSO) is the strongest signal of global interannual climate anomaly and reconstructing past ENSO variations using high-resolution paleoclimate archives can improve our understanding of ENSO variability, as well as improve our ability to predict future climate changes. Here, a daily resolution standardized growth index (SGI) was established using a giant clam (Tridacna spp.) shell specimen MD2 (life span: 1994-2013 CE), collected from the Yongshu Reef, southern South China Sea (SCS). The cross-spectral and correlation analysis indicated that the SGI variation of MD2 was strongly influenced by ENSO variability on an interannual timescale. Tridacna spp. is in symbiosis with zooxanthellae, and its growth index is usually modulated by the photosynthetic efficiency of zooxanthellae. During the El Niño (La Niña) period, the convective anomalies stimulated in western Pacific would increase (decrease) the effective solar radiation on Yongshu Reef, and in turn influence the photosynthesis rate of zooxanthellae and enzyme activity for the calcification site and thus the SGI of giant clam MD2. The SGI can explain 54.7 % of ENSO variance, demonstrating the potential for Tridacna SGI in ENSO reconstruction. Compared with conventional ENSO reconstruction using high-resolution geochemical proxies, the method of giant clam SGI is rapid and economical.

Off-season survival and life history of beet armyworm, Spodoptera exigua (Hubner) on various host plants.

The beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae), has become a significant pest of chickpea in recent years. The polyphagous nature allows it to survive on various hosts during the off-season, creating a great menace to the crop in the following season. To assess the incidence and document the alternate hosts of S. exigua, a rapid roving survey was conducted in 11 chickpea-growing areas of Prakasam district, Andhra Pradesh, India. Additionally, the life history traits of S. exigua were studied on major alternate host plants under laboratory conditions (27 ± 1 °C and 70 ± 2% RH) to understand the survival, life expectancy and potential contribution to future populations. The results show that, among the different crops surveyed, the maximum larval incidence was noticed in maize (1.93 larvae/plant), cowpea (1.73 larvae/plant), and sunflower (1.68 larvae/plant) during the off-season. Life history studies of S. exigua showed that highest larval survival percentage was observed on chickpea (83.6%), while the lowest was on maize (44.5%). The mean developmental time for larvae was longest on maize (27.1 days) and shortest on chickpea (14.9 days). Larvae did not develop beyond the third instar when fed with chilli. The growth index statistics showed chickpea (9.2) was the most suitable host plant, whereas maize (0.9) was the least suitable host. The age-stage-specific survival rate (Sxj) varied across developmental stages, and the survival curves overlapped, indicating different growth rates among individuals. The life expectancy (exj) at age zero was highest on groundnut (37.06 days). The intrinsic rate of increase (r) of S. exigua was lowest on maize (0.10 ± 0.0013) and highest on chickpea (0.22 ± 0.0010). Similarly, the net reproductive rate (R0) was highest on chickpea (846.39 ± 18.22) and lowest on maize (59.50 ± 2.06). The population doubled every 3.08 ± 0.011 days on chickpea compared to 7.22 ± 0.80 days on maize. The study conclusively indicates that chickpea and sunflower, primarily cultivated during the rabi season in India, are the most preferred hosts for S. exigua. In contrast, maize and cotton, mainly grown during the kharif season, are less preferred and merely support the pest's survival. Consequently, S. exigua switches hosts between different crops growing seasons, so effective management of S. exigua during the kharif season can help prevent pest outbreaks during the rabi season.

Comparative analysis of physiological responses to environmental stress in Hedysarum scoparium and Caragana korshinskii seedlings due to roots exposure.

Hedysarum scoparium and Caragana korshinskii seedlings were used as the test materials to carry out the roots exposure stress model test. By comparing the physiological growth indexes in the leaves of the tested plants, the stress resistance ability was evaluated. The results showed (1) root exposure resulted in excessive oxygen free radicals, which resulted in membrane lipid peroxidation and increased MDA content in two plants. The increase of MDA content in H. scoparium was greater than that in C. korshinskii. (2) H. scoparium mainly regulate their own adaptation to the stress by regulating carotenoids. C. korshinskii adjusts itself to adapt to the stress by regulating chlorophyll. (3) H. scoparium resist this stress mainly by regulating their respiration rate. (4) H. scoparium mainly through the mobilization of proline, by adjusting the proline concentration to reduce their water potential; C. korshinskii reduced its water potential mainly by regulating the concentration of soluble sugar to adapt to the stress. (5) H. scoparium and C. korshinskii activated peroxidase (H. scoparium) and catalase (C. korshinskii) to clean up intracellular peroxides, respectively. To sum up, under the same root exposure ratio, there were significant differences in physiological regulation and morphological indexes between H. and C. korshinskii, but their stress resistance mechanisms were quite different.