ABSTRACT
<p><b>OBJECTIVE</b>To prepare flexible proanthocyanidins nanoliposomes, and explore the in vitro release behavior of proanthocyanidins flexible nanoliposomes and general nanoliposomes.</p><p><b>METHOD</b>Flexible proanthoeyanidins nanoliposomes were prepared proanthocyanidins using a film dispersion method, characterized by transmission electron microscope, and the in vitro release action was studied in different dissolution mediums using dynamic dialyse method with the content of total phenol as index.</p><p><b>RESULT</b>The in vitro release of both proanthocyanidins flexible nanoliposomes and general nanoliposomes were in accordance with Weibull distribution.</p><p><b>CONCLUSION</b>Proanthocyanidins flexible nanoliposomes without pressure had similar in vitro release behavior with general nanoliposomes.</p>
Subject(s)
Drug Delivery Systems , Methods , Liposomes , Chemistry , Nanospheres , Chemistry , Particle Size , Proanthocyanidins , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the regularity of Yulian Cataplasm in vitro release and transdermal behaviors.</p><p><b>METHOD</b>Improved Franz diffusion devices was used with four index ingredients as evodiamine, rutaecarpine, palmatine and berberine that were determined by HPLC in one mobile phase.</p><p><b>RESULT</b>The release rates of evodiamine, rutaecarpine, palmatine and berberine were 0.0239, 0.0156, 0.0725, 0.8191 mg x cm(-2) x h(-1/2), respectivley. The transdermal rates of evodiamine, rutaecarpine, palmatine and berberine were 1.256, 1.0302, 2.8029, 20.919 microg x cm(-2) x h(-1), respectively.</p><p><b>CONCLUSION</b>The releasing process of all index is in accordance with Higuchi equation and the transdermal proccess is in accordance with zero-level equation.</p>
Subject(s)
Animals , Mice , Berberine , Pharmacokinetics , Berberine Alkaloids , Pharmacokinetics , Drugs, Chinese Herbal , Pharmacokinetics , Indole Alkaloids , Pharmacokinetics , Plant Extracts , Pharmacokinetics , Quinazolines , Pharmacokinetics , Skin , Metabolism , Skin AbsorptionABSTRACT
<p><b>OBJECTIVE</b>To study the preparation ofnanostructured lipid carriers loaded with supercritical carbon dioxide fluid extraction of Xionggui powder.</p><p><b>METHOD</b>Prepared nanostructured lipid carriers (NLC) loaded with supercritical carbon dioxide fluid extraction of Xionggui powder (XG-CO2-SFE) with a microemulsion ultrasonication method , established the best prescription of XG-CO2-SFE-NLC by orthogonal design methods with entrapment efficiency of nanoparticles as index, and investigated their physicochemical characterizations.</p><p><b>RESULT</b>The best prescription was m(phospholipid) : m(S-40) : m(lipid carriers) = 5 : 2 : 1, the entrapment efficiency of nanopartices was 86.8%, results revealed that nanoparticles were sphere like with the mean size of 92.6 nm and the mean zeta potential was -20.68 mV.</p><p><b>CONCLUSION</b>The prep aration method of the XG-CO2-SFE-NLC is appropriate.</p>
Subject(s)
Carbon Dioxide , Chemistry , Chromatography, Supercritical Fluid , Drug Carriers , Chemistry , Drugs, Chinese Herbal , Chemistry , Emulsions , Linear Models , Lipids , Chemistry , Nanostructures , Chemistry , PowdersABSTRACT
<p><b>OBJECTIVE</b>To prepare and evaluate the injectable temperature responsive in situ gel for sustained release of Glabrous Sarcandra Herb extract in vitro.</p><p><b>METHOD</b>The novel temperature responsive poloxamer 407 was used as the carrier material of Glabrous Sarcandra Herb extract sustained release injection. The release of Glabrous Sarcandra Herb extract from poloxamer 407 based in situ gel in the phosphate buffer (pH 7. 6) was studied at 37 degrees C under agitation. HPLC method was used for the determination of Glabrous Sarcandra Herb extract.</p><p><b>RESULT</b>The best prescription was composed of by 16% P-407, 6% P-188, 0.2% HPMC, 0.5% benzil alcohol and 4% (g mL(-1)) Glabrous Sarcandra Herb extract. The optimal gelation temperature was around 33 degrees C. The data of release in vitro were analyzed according to the theoretical model of Korsmeyer-Peppas.</p><p><b>CONCLUSION</b>The preparation method of the injectable thermosensitive in situ gel of Glabrous Sarcandra Herb extract is appropriate. The release in vitro can reach the expecting request.</p>
Subject(s)
Ferns , Chemistry , Plant Extracts , Poloxamer , Chemistry , TemperatureABSTRACT
<p><b>OBJECTIVE</b>To optimize the matrix formulation of cataplasm.</p><p><b>METHOD</b>Face-centered design was used in the experimental design, and response surface was produced in quadratic polynomial after data fitting in order to explore the impacts of Sodium Polyacrylate, Carbomer and the cross-linking agent on stickiness of cataplasm, optimize the prescription of the cataplasm matrix and perform the evaluation analysis.</p><p><b>RESULT</b>The multiple correlation coefficient (R2) and adjusted R2 in the fitting method using quadratic polynomial were 0.970 and 0. 952 (F = 53.953, P = 0.0001), respectively, and the model was significant different. The ratio of optimum proportion of Sodium Polyacrylate, Carbomer and the cross-linking agent in the matrix of cataplasm was determined, which was proved efficaciously.</p><p><b>CONCLUSION</b>Face-centered design-response surface method is a simple method with good prediction result for the optimization of cataplasm matrix.</p>
Subject(s)
Chemistry, Pharmaceutical , Methods , Drugs, Chinese Herbal , Chemistry , Models, TheoreticalABSTRACT
Objective To establish a method for the determination of Bullatine A in Gutongling Babu plaster. Method The Bullatine A was determined by HPLC on Kromasil-C18 column, and the mobile phase consisted of phosphate buffered solution (pH 7.3) - methanol (28∶72), the detection wavelength was 210 nm. Result A good linear relationship of Bullatine A was showed in the range of 0.212 0～4.770 0 ?g (r =0.999 99), and the average recovery was 100.10%, RSD=2.53% (n =6). Conclusion The method is accurate and reliable, and can be used for quality control of Gubitong Babu plaster.