ABSTRACT
OBJECTIVE@#To explore the expression of fibroblast activation protein alpha (FAPalpha) and transforming growth factor-beta1 (TGF-beta1) in myocardial cytoplasm for the cases of sudden death due to acute myocardial ischemia.@*METHODS@#The heart tissues of 47 cases were collected. All cases were divided into three groups: control group, acute myocardial infarction group and recurrent myocardial infarction group. FAPalpha and TGF-beta1 expressions were explored in myocardial cytoplasm by immunohistochemistry technology. The staining results were collected by image analysis system and then the positive area ratio and average optical density were detected. The positive signal differences were compared among the groups.@*RESULTS@#Strong FAPalpha and TGF-beta1 expressions were detected in myocardial cytoplasm in both acute and recurrent myocardial infarction groups. The expression of FAPalpha was not detected in myocardial cytoplasm in control group and TGF-beta1 expression showed a weak positive result. FAPalpha and TGF-beta1 expressions showed the statistical difference (P < 0.05) in myocardial infarction (acute and recurrent) groups and control group.@*CONCLUSION@#FAPalpha and TGF-beta1 can be the diagnostic markers for determing acute myocardial infarction.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Acute Disease , Autopsy , Death, Sudden, Cardiac , Endopeptidases , Forensic Pathology , Gelatinases/metabolism , Immunohistochemistry , Membrane Proteins/metabolism , Myocardial Infarction/pathology , Myocardial Ischemia/pathology , Myocardium/metabolism , Myocytes, Cardiac/metabolism , Recurrence , Retrospective Studies , Serine Endopeptidases/metabolism , Transforming Growth Factor beta1/metabolismABSTRACT
OBJECTIVE@#To investigate the expression of heme oxygenase-1 (HO-1) at different intervals and to provide evidence for estimation on injury intervals after brain contusion in human.@*METHODS@#Twenty-four patients died of serious brain injury were assigned as injury group and 4 patients died of non-brain injury were served as control group. HO-1 expression was analyzed in brain tissue at different time intervals (3 h, 6-9 h, 12-24 h, 36 h-3d, 5-8d, 17-20d) by immunohistochemistry and auto-image analysis system.@*RESULTS@#The level of HO-1 expression started to increase in 3 h after brain contusion compared to the control group (P < 0.05). The level of HO-1 expression highest level in 12-24 h group, and maintained high level in 36 h-3 d, then decreased gradually.@*CONCLUSION@#The expression of HO-1 might be a strong evidence for human brain contusion time estimation.