Recent Concepts of Guillain-Barré Syndrome

Guillain-Barré syndrome (GBS) is a representative form of post-infectious autoimmune neuropathy with heterogenous manifestations. It was originally considered as an ascending demyelinating polyneuropathy in Western countries. However, the discovery of anti-ganglioside antibodies on the basis of molecular mimicry theory could help us better understand various kinds of focal and regional variants as well as axonal type of GBS those were frequently found from Asian countries. Recent development of new techniques about anti-ganglioside complex antibodies is making more detailed descriptions for specific or unusual clinical manifestations. It has been regarded that GBS has good prognosis if treated properly as early as possible, but it still shows high mortality and morbidity rate with frequent long term neurologic and medical complications. Unfortunately, there are only two options for medical treatment, intravenous immunoglobulin and plasmapheresis, for the last 100 years. Several clinical studies on new immunotherapy targeting complement activating system with background of molecular mimicry using animal model are underway. We hope that these new treatments will be helpful for the future patients.

A Variant Guillain-Barré Syndrome with Anti-Ganglioside Complex Antibody

BACKGROUND: Recently, anti-ganglioside complex (GSC) antibodies were discovered among the various subtypes of Guillain-Barré syndrome. GSC is the novel glycoepitopes formed by two individual ganglioside molecules. CASE REPORT: We present a 36-year-old man with overlap Miller Fisher syndrome and acute bulbar palsy who had anti-GSC antibody that provided diagnostic robustness. CONCLUSION: Anti-GSC testing could be considered important in patients who show atypical manifestation with negative antibody reaction against each constituent ganglioside.

Apoptosis Effects of GM3 Ganglioside on U266 Cells and Its Possible Mechanism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the proliferation- inhibitory and apoptosis inducing effect of ganglioside GM3 on human multiple myeloma cell line U266 cells and its possible mechanisms.</p><p><b>METHODS</b>MTT assay and flow cytometry were used to observe the effects of GM3 ganglioside on proliferation and apoptosis of human myeloma cell line U266. Effects of different concentration of ganglioside GM3 on the mRNA expression level of BCL-2 and BAX were detected by Real-time PCR.</p><p><b>RESULTS</b>MTT assay and Flow Cytometry showed that ganglioside GM3 could induce the apoptosis and inhibit the proliferation of multiple myeloma U266 cell line, and both the effects were enhanced with the increase of GM3 ganglioside concentration. Compared with the control group, the relative expression of BAX mRNA with the increase of GM3 concentration in experimental group was enhanced gradually(r=0.968), while the relative mRNA expression of anti-apoptotic gene BCL-2 was decreased gradually（r=-0.727).</p><p><b>CONCLUSION</b>GM3 ganglioside can induce apoptosis and inhibit the proliferation of U266 cell line in a concentration dependent manner. The mechanism may be related with up- regulation of BAX expression and down-regulation of BCL-2.</p>

Gangliosidose GM1 infantil ­ relato de caso / Child GM1 Gangliosidosis ­ Case Report

Introdução: A gangliosidose é uma doença caracterizada pelo acúmulo do substrato gangliosídeo nos lisossomos devido à deficiência da enzima betagalactosidase. É uma desordem rara, estimando-se uma incidência na população de 1:100.000 a 200.000. Clinicamente os pacientes apresentam graus variados de neurodegeneração e alterações esqueléticas, categorizadas pela gravidade e atividade residual da beta-galactosidase, podendo ocorrer dismorfismo facial, hepatoesplenomegalia, displasia esquelética, manchas maculares vermelhas, cegueira e até morte precoce. O atraso no desenvolvimento neuropsicomotor associada à degeneração do sistema nervoso central, pode levar o paciente a um quadro de hipotonia muscular generalizada progressiva, evoluindo para espasticidade e crises convulsivas. Objetivo: Relata-se caso de paciente masculino, apresentando alterações no desenvolvimento neuropsicomotor desde os oito meses, com elucidação diagnóstica através da clínica, exames de imagem e laboratoriais. Método: Busca em bancos de dados digitais artigos científicos que discorram sobre a gangliosidose. Resultados/Conclusão: A gangliosiodose é uma disordem rara, o que torna o relato de caso importante como fonte de pesquisa. (AU)

Introduction: Gangliosidosis is a disease characterized by accumulation of the ganglioside substrate in lysosomes due to beta-galactosidase enzyme deficiency. It is a rare disorder, with an incidence of 1: 100,000 to 200,000. Clinically the patients pres- ent varying degrees of neurodegeneration and skeletal changes, categorized by the gravity and residual activity of beta-galactosidase, being able to occur facial dysmorphism, hepatosplenomegaly, skeletal dysplasia, red macular spots, blind- ness and early death. Objective: Delayed neuropsychomotor development associated with degeneration of the central nervous system may lead the patient to progressive generalized muscular hypotonia, evolving into seizures and convulsive seizures. We report a case of male patient, presenting changes in neuropsychomotor devel- opment since eight months of age, with diagnostic elucidation through clinical exam, imaging and laboratory tests. Method: Search in digital databases for scientific articles that discuss gangliosidoses. Results/ conclusion: Gangliosidosis is rare disorder, which makes reporting an important source of research. (AU)

Clinical effects of Ganglioside and fructose-1, 6-diphosphate on neonatal heart and brain injuries after Asphyxia

Objective: To study the clinical effect of ganglioside [GM] and fructose-1, 6-diphosphate [FDP] on neonatal heart and brain injuries after asphyxia

Methods: Ninety-one neonates with asphyxia neonatal heart and brain injuries were randomly divided into an observation group and a control group. Both groups were given symptomatic treatment as soon as possible. On this basis, the observation group was given 200 ml of 5% glucose injection and 20 mg of GM and 250 mg/kg-d FDP by intravenous infusion. The above two drugs were given once a day for 14 days. The control group was given 20 ml of 5% glucose injection, 2 ml of cerebrolysin and 250 mg/kg-d FDP by intravenous infusion, once a day for 14 days. Both groups were administered on the first day after admission, and the course of treatment was 14 days. The treatment outcomes of the two groups were compared by detecting the levels of glycogen phosphorylase isoenzyme BB [GPBB], cTn-l and CK-MB, MRI results and Neonatal Behavioral Neurological Assessment [NBNA] scores before and after treatment

Results: The levels of GPBB, cTn-l and CK-MB in the observation group were significantly higher than those of normal neonates. After treatment, the levels of cTn-l and CK-MB in the observation group were closer to those of normal neonates compared with the control group, with significant differences [P<0.05]. There was a significant difference in the brain MRI examination between the two groups [P<0.05]. The NBNA scores of the two groups were significantly different before and after treatment [P<0.05]. The total effective rate of the observation group was significantly higher than that of the control group [P<0.05]. Conclusion: Neonatal heart and brain injuries after asphyxia can be well treated by combining GM with FDP

Co-occurrence of Guillain-Barré Syndrome and Acute Disseminated Encephalomyelitis with Dual Positive of Anti-GT1a and Anti-GM1 Antibodies

Acute disseminated encephalomyelitis (ADEM) and Guillain-Barré syndrome (GBS) are both rare post-infectious neurological disorders. The co-existence of these conditions has often been reported despite of low incidence. We describe a 20-year-old male, who presented with acute flaccid paralysis and encephalopathy. The patient showed reversible MRI lesions suggesting ADEM. This case showed anti-GT1a IgG and anti-GM1 IgM antibodies positivity. We suggest that certain immunogenicity within central and peripheral nervous system may share a common autoimmune process during the disease course.

Lentivirus-mediated microRNA-124 gene-modified bone marrow mesenchymal stem cell transplantation promotes the repair of spinal cord injury in rats

Our study aims to explore the effects of lentivirus-mediated microRNA-124 (miR-124) gene-modified bone marrow mesenchymal stem cell (BMSC) transplantation on the repair of spinal cord injury (SCI) in rats. BMSCs were isolated from the bone marrow of rats. The target gene miR-124 was identified using a luciferase-reporter gene assay. Seventy-two rats were selected for construction of the SCI model, and the rats were randomly divided into the blank group, sham group, SCI group, negative control (NC) group, overexpressed miR-124 group and si-PDXK group. The mRNA expression of miR-124 and the mRNA and protein expression of pyridoxal kinase (PDXK) were detected by quantitative real-time polymerase chain reaction and western blotting. The locomotor capacity of the rats was evaluated using the Basso, Beattie and Bresnahan (BBB) scale. Brdu, neuron-specific enolase (NSE), neurofilament (NF) and microtubule-associated protein 2 (MAP2) were detected using immunohistochemistry. The expression levels of thyrotropin-releasing hormone (TRH), prostacyclin (PGI2) and gangliosides (GM) were measured using an enzyme-linked immunosorbent assay. PDXK was identified as the target gene of miR-124. The overexpressed miR-124 group exhibited higher miR-124 expression than the SCI, NC and si-PDXK groups. Compared with the SCI and NC groups, the PDXK expression was downregulated in the overexpressed miR-124 and si-PDXK groups, and the BBB scores were significantly increased 7, 21 and 35 days after transplantation. The double-labeled positive cell densities (Brdu+NSE/NF/MAP2) and the expression levels of TRH, PGI2 and GM in the overexpressed miR-124 group were significantly higher than those in the NC and SCI groups. These results indicated that miR-124 targeted PDXK to accelerate the differentiation of BMSCs into neurocytes and promote SCI repair.

Recurrent Ophthalmoplegia Presenting Different Clinical Features in a Patient with Anti-GQ1b Antibody Syndrome

No abstract available.

Effects of gangliosides from deer bone extract on the gene expressions of matrix metalloproteinases and collagen type II in interleukin-1β-induced osteoarthritic chondrocytes

BACKGROUND/OBJECTIVES: We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-1β-induced osteoarthritis (OA) chondrocytes. MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-1β treatment), PC (IL-1β + 100 µg/mL glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-1β + 100 µg/mL deer bone extract). RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to 500 µg/mL inhibits cell death in chondrocytes induced by IL-1β. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-1β (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05). CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1β-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.

Autoantibody-Mediated Sensory Polyneuropathy Associated with Indolent B-Cell Non-Hodgkin's Lymphoma: A Report of Two Cases

BACKGROUND AND PURPOSE: Abnormalities of the peripheral nervous system occur in 5% of patients with lymphoma. Polyneuropathy has not been described in patients with mantle-cell and marginal-zone B-cell lymphomas. CASE REPORT: Two elderly patients with indolent non-Hodgkin's lymphoma developed a progressive sensory polyneuropathy that was associated with serum autoantibodies directed against asialosyl/sialosyl gangliosides and myelin-associated glycoprotein/sulfated glucuronyl paragloboside, respectively, which are peripheral-nerve antigens. The oligoclonal pattern of these antibodies hinted at a lymphoma-induced immune dysregulation. The neuropathy stabilized clinically during treatment with intravenous immunoglobulin G. B-cell lymphoma was managed with a "watchful waiting" approach. CONCLUSIONS: The concept of antigen-specific, immune-mediated neuropathy associated with slow-growing lymphoma of mature B-cells may be underrecognized. The principle of treating the illness underlying neuropathy may not be always indicated or necessary if risk-benefit and cost-benefit analyses are taken into account.

Efficacy and safety of phosphodiesterase inhibitors for erectile dysfunction in diabetic men: A meta analysis / 中华男科学杂志

<p><b>OBJECTIVE</b>To evaluate the clinical efficacy and safety of phosphodiesterase 5 (PDE-5) inhibitors for erectile dysfunction (ED) in patients with diabetes mellitus and provide some evidence for the clinical treatment of the disease.</p><p><b>METHODS</b>We searched MedMed, EMbase, Cochrane Library, CNKI, Wan Fang Data, VIP and ZADL for randomized controlled trials on PDE-5 inhibitors for ED in diabetic men and evaluated the methodology of the included trials with the Jadad scale. We used the erectile function domain in the IIEF (IIEF-EF), IIEF questions (IIEF-Q) 3 and 4, SEP-2 and -3, and Global Assessment Questions (GAQ) as the main evaluation indexes and employed the Review Manager 5. 1. 0 software for meta analysis.</p><p><b>RESULTS</b>A total of 13 studies were included, which were all high quality trials with Jadad score > 3. The IIEF-EF scores in 10 of the included studies were subjected to meta analysis using the random-effect model (REM), with a weighted mean difference (WMD) of 5.64 (95% CI 4.41 - 6.83, P < 0.001). The fixed-effect model (FEM) analysis of the IIEF-Q scores in 6 of the studies showed the WMD to be 0.96 (95% CI 0.83 -1.08, P < 0.001) for IIEF-Q3 and 1.11 (95% CI 0.98 - 1.25, P < 0.001) for IIEF-Q4. FEM analysis of the SEP-2 scores showed WMD = 17.67 (95% CI 12. 38 - 22. 97, P < 0.001) in 2 of the studies, and that of the SEP-3 scores WMD = 23.64 (95% CI 17. 49 - 29.79, P < 0.001) in 5 of the studies. The GAQ scores in 11 of the studies were subjected to REM analysis, with OR = 6. 20 and 95% CI 3.65 - 10.52 (P < 0.001). REM analysis was performed on the adverse reactions in 11 of the studies, with OR = 7.43 and 95% CI 4.11 - 13.44 (P < 0.001).</p><p><b>CONCLUSION</b>PDE-5 inhibitors can effectively and safely improve erectile function in patients with diabetes mellitus.</p>

Treatment of cerebral palsy children by integrative medical sequential method: a clinical efficacy observation / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the efficacy of integrative medical sequential method in treating cerebral palsy (CP) children's intelligence development, muscular tension, serum interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-alpha).</p><p><b>METHODS</b>Totally 111 CP children were randomly assigned to the control group (50 cases) and the treatment group (61 cases). All patients received comprehensive rehabilitation training and intravenous dripping of Monosialotetrahexosylganglioside Sodium Injection for 10 days. But those in the treatment group additionally received Chinese medical enema for brain resuscitation, relieving rigidity of muscles and activating collaterals for 14 days. Then they started another medication cycle and lasted for a total of 6 cycles. Serum IL-6 levels and TNF-alpha contents were determined before treatment. Scoring for muscular tension, Gesell score for intelligence development, contents of serum IL-6 and TNF-alpha were assessed before and after treatment in the two groups.</p><p><b>RESULTS</b>Compared with before treatment in this group, muscular tension, Gesell scores for intelligence development all decreased in the two groups (P < 0.05). As for inter-group comparison, the decrement was more obvious in the treatment group than in the control group (P < 0.05). The total effective rate was 86.9% in the treatment group and 76.0% in the control group (P < 0.05). The contents of IL-6 and TNF-alpha were obviously reduced in the treatment group and the control group after treatment (P < 0.01). The decrement was more obvious in the treatment group (P < 0.05).</p><p><b>CONCLUSION</b>The two treatment methods were effective for CP children, but the efficacy was superior in the treatment group than in the control group, indicating integrative medical methods could play a synergistic effect and optimize the treatment program for CP.</p>

Síndrome anti-GQ1b: Descripción de cuatro pacientes y revisión de la literatura / Anti-GQ1b syndrome: Report of four cases

Anti-GQ1b syndrome includes Miller Fisher Syndrome (MFS), Guillain Barré Syndrome (GBS), Bickerstaff`s brain stem encephalitis (BBE) and Acute Ophtamoplegia (AO). We report four patients aged 16 to 76 years, with anti-GQ1b syndrome. All presented with MFS, one of them evolved to GBS pharyngeal-cervical-brachial variant and other to GBS with BBE. All had a previous history of diarrhea or upper respiratory tract infection. All had positive anti-GQ1b serum antibodies. Both brain magnetic resonance imaging and cerebrospinal fluid analysis were normal. Electrophysiology studies were compatible with a demyelinating disease. Two patients needed airway protection with an orotracheal tube and developed dysautonomia. All four patients were treated with immunomodulation. On the sixth month follow-up, patients had only minimal alterations in the neurological examination.

An Adult Case of Fisher Syndrome Subsequent to Mycoplasma pneumoniae Infection

Reported herein is an adult case of Fisher syndrome (FS) that occurred as a complication during the course of community-acquired pneumonia caused by Mycoplasma pneumoniae. A 38-yr-old man who had been treated with antibiotics for serologically proven M. pneumoniae pneumonia presented with a sudden onset of diplopia, ataxic gait, and areflexia. A thorough evaluation including brain imaging, cerebrospinal fluid examination, a nerve conduction study, and detection of serum anti-ganglioside GQ1b antibody titers led to the diagnosis of FS. Antibiotic treatment of the underlying M. pneumoniae pneumonia was maintained without additional immunomodulatory agents. A complete and spontaneous resolution of neurologic abnormalities was observed within 1 month, accompanied by resolution of lung lesions.

Elevated in Anti-GQ1b and Anti-GT1a IgG Antibody Titers in an Overlap Case of Pharyngeal-Cervical-Brachial Variant of Guillain-Barre Syndrome and Miller-Fisher Syndrome / 대한임상신경생리학회지

No abstract available.

A Patient Presented With Unilateral Abducens Nerve Palsy: A Variant Form of Guillain-Barre Syndrome With Anti-GT1a Antibody

No abstract available.

The observation on curative effect of 90 cases of sudden hearing loss / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the efficiency between two treatments of sudden hearing loss.@*METHOD@#All patients were divided into two groups randomly, basic drug group was treated with ganglioside and vinpocetine injection, combined therapy group was treated with intratympanic dexamethasone and what was used in basic drug group.@*RESULT@#The effective rate of combined therapy group (73.53%) was significantly higher than that of basic drug group (37.78%) (P < 0.05).@*CONCLUSION@#The comprehensive therapy of intratympanic dexamethasone injection, ganglioside and vinpocetine injection have excellent efficiency for sudden hearing loss.

Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells / 한국실험동물학회지

Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-alpha. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-alpha (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-alpha. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.

Estudo dos efeitos do monossialogangliosídeo (GM1) administrado pela via transdérmica por laser a baixa temperatura, após lesão medular experimental em ratos

Objetivo: avaliar os efeitos do monossialogangliosídeo (GM1) administrado pela via de transdérmica por laser a baixa temperatura, após lesão medular experimental em ratos. Métodos: o estudo incluiu 40 ratos Wistar, machos, com idade entre 20 e 21 semanas, submetidos à lesão medular contusa pelo equipamento NYU Impactor, a altura de 25 mm, de acordo com o protocolo MASCIS. Foram formados 4 grupos de 10 animais. No grupo 1, os ratos receberam diariamente 0,2 ml de soro fisiológico, via intraperitoneal; no grupo 2, GM1 via intraperitoneal, na concentração 30 mg/kg por dia; no grupo 3, sessão diária de laser a baixa temperatura na topografia da lesão; no grupo 4 sessão diária de laser contendo GM1 na concentração de 30 mg/kg pela via transcutânea por Laser Ice. Todos os animais foram tratados por 42 dias. Foram avaliados por meio da escala de avaliação funcional Basso, Baettie e Bresnahan (BBB) em 7, 14, 21, 28, 35 e 42 dias após a lesão, pelo exame histopatológico e por potencial evocado motor após 42 dias da lesão. Resultados: os animais do grupo 4 apresentaram os escores da escala BBB superiores aos demais grupos até a quarta semana, sendo equiparado aos demais na sexta semana. Não houve diferença estatisticamente significante entre os grupos e as semanas. A avaliação histológica não demonstrou resultados com significância estatística. Os exames de potencial evocado motor demonstraram maior latência média no grupo 1, sem significância estatística...

Objective: To evaluate the effects of monossialoganglioside (GM1) administered transdermally, and laser at low temperature, in the functional and histological recovery of spinal cord injury in rats. Methods: Forty male Wistar rats, aged between 20 and 21 weeks, underwent spinal cord contusion at NYU Impactor, according to the MASCIS protocol. They were divided into four groups: in Group 1, rats received 0.2 ml of saline intraperitoneally daily; in Group 2, GM1 was administered intraperitoneally at a concentration 30 mg/kg per day; in Group 3, rats were treated with laser at low temperature on the skin, daily and in Group 4, the daily laser session also contained GM1. All the groups were treated for 42 days. The animals were evaluated by the Basso, Baettie and Bresnahan (BBB) functional scale on days 7, 14, 21, 28, 35 and 42 after injury, and by histopathology and motor evoked potentials after 42 days of injury. Results: The animals in Group 4 had higher BBB scores compared to the other groups, until the 4th week. There were no statistically significant differences between the groups, or in the comparisons over time, i.e. from one week to the next. Histological evaluation showed no statistically significant results, and no significant differences were found in the motor evoked potential tests either. Conclusion: GM1 associated with the use of low-temperature laser shows no superior functional, neurological or histological results in the treatment of spinal cord lesions in rats...

Relationship between ganglioside expression and anti-cancer effects of the monoclonal antibody against epithelial cell adhesion molecule in colon cancer

The human colorectal carcinoma-associated GA733 antigen epithelial cell adhesion molecule (EpCAM) was initially described as a cell surface protein selectively expressed in some myeloid cancers. Gangliosides are sialic acid-containing glycosphingolipids involved in inflammation and oncogenesis. We have demonstrated that treatment with anti-EpCAM mAb and RAW264.7 cells significant inhibited the cell growth in SW620 cancer cells, but neither anti-EpCAM mAb nor RAW264.7 cells alone induced cytotoxicity. The relationship between ganglioside expression and the anti-cancer effects of anti-EpCAM mAb and RAW264.7 was investigated by high-performance thin-layer chromatography. The results demonstrated that expression of GM1 and GD1a significantly increased in the ability of anti-EpCAM to inhibit cell growth in SW620 cells. Anti-EpCAM mAb treatment increased the expression of anti-apoptotic proteins such as Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-alpha, caspase-3, cleaved caspase-3, and cleaved caspase-8 were unaltered. We observed that anti-EpCAM mAb significantly inhibited the growth of colon tumors, as determined by a decrease in tumor volume and weight. The expression of anti-apoptotic protein was inhibited by treatment with anti-EpCAM mAb, whereas the expression of pro-apoptotic proteins was increased. These results suggest that GD1a and GM1 were closely related to anticancer effects of anti-EpCAM mAb. In light of these results, further clinical investigation should be conducted on anti-EpCAM mAb to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.