Analyzing the neuropsychological characteristics and changes in serum markers of patients with chronic cerebral circulation insufficiency

Summary Objective: To investigate the neuropsychological characteristics and changes in CRP, S100B, MBP, HSP-7, and NSE in serum. Method: Sixty-six (66) patients treated in our hospital as CCCI group were chosen for our study, and 90 patients with depression were selected as the depression group. The patients in both groups were examined with CT perfusion, depression, anxiety and cognition evaluation. Their serum CRP, S100B, MBP, HSP-70 and NSE levels were detected. Neuropsychological and serum markers characteristics were compared. Results: The CBF and CBV in bilateral basal ganglia, frontal lobes, greater oval center, brain stem, and left and right regions of occipital lobes of the patients in CCCI group were significantly lower than in the depression group. The HAMD and HAMA scores of CCCI group patients were significantly lower than in the depression group; CCCI group performed better regarding attention, memory, abstract terms and delayed recall. CCCI also had significantly higher total scores than the depression group. Serum CRP, S100B, MBP, HSP-70 and NSE levels in CCCI group were significantly higher than in the depression group. The differences reach statistical significance (p<0.05). Conclusion: CCCI patients who are accompanied by minor depressive disorder have different degrees of cognitive impairment and experience a significant rise in serum CRP, S100B, MBP, HSP-70 and NSE.

Serum heat shoch protein 70 levels in pre-eclampsia and adverse pregnancy outcomes

Heat shock protein 70 [Hsp 70], a marker of cellular stress, was suggested to be elevated in pregnancies complicated by pre-eclampsia. Adverse pregnancy outcomes [APOs] are a group of common obstetric diseases and many studies have been conducted in an effort to clarify their risk factors It is well known that these risk factors can induce the synthesis of a group of highly conserved proteins, called heat shock proteins [Hsps]. The aim of the study was to measure serum heat shock protein [Hsp]70 in mothers with pre-eclampsia and adverse pregnancy outcomes [APOs] and to evaluate whether it can be applied as a useful indicator for the development of these conditions. Eighty pregnant women were included in this study [20 pre-eclamptic women, 20 women with threatened preterm labor, 20 women with intrauterine growth restriction and 20 healthy women with non-complicated pregnancy]. After obtaining informed consent, serum samples were collected from all participants to measure Hsp70 levels. The levels of Hsp 70 were measured using enzyme-linked immunosorbent assay. Measurement of serum Hsp 70 levels showed statistically higher values among pre-eclamptic patients compared to preterm, Intrauterine growth restriction [IUGR] and control groups [24.6 +/- 12.7 ng/ml, 15.l +/- 5.4 ng/ml, 14.3 +/- 6.1 ng/ml, 11 .7 +/- 4.9 ng/ml respectively, p

study of the effect of estrogen hormonal modulation on liver ischemic reperfusion in rats

Ischemia/reperfusion [I/R] injury of the liver impaired hepatic regeneration and predisposed to liver failure. The prime factors contributing to injury are decreased nitric oxide [NO] level which favor vasoconstriction, increased production of reactive oxygen species [ROS], increased level of adhesion molecules and leukostasis, which further impair the hepatic microcirculation in the early reperfusion phase. Heat shock protein 70 [HSP70] was shown to be induced by stress. Of the present work was to study the effect of estrogen hormonal modulation on hepatic injury during I/R model in male rats and to investigate the possible involvement of HSP 70 in the gender dimorphic response of the liver to this injury. Forty adult male albino rats were used in this work. They were divided into 5 groups [each of eight rats]; eight rats were taken as normal sham operated group.The remaining rats were subjected to total hepatic I/R. Each group received a specific treatment given intraperitoneally [ip] including: 17-beta estradiol [E[2]], raloxifene [Ral.] and genistein [Gen.] one hour before surgical procedure. The sham operated rats were injected with the veichle dimethyl sulfoxide solution. The I/R group was pretreated with the vehicle. Several parameters including: serum transaminases, HSP 70, ROS, Myeloperoxidase activity[MPO] and nitrite content were measured using standard assay procedures. I/R produced a significant increase in serum transaminases, MPO and ROS in liver tissue. HSP 70 was induced to a significantly higher level in I/R versus sham operated group but nitrite was significantly reduced in I/R versus sham operated rats. E[2+] I/R group had significantly lower transaminases, MPO and ROS versus I/R untreated group. A significantly higher nitrite level and HSP 70 was detected in liver tissue of E[2+] I/R versus I/R group. Ral.+I/R group showed significant lower serum transaminases, MPO and ROS in liver tissue versus I/R. Also Ral+ I/R showed significant higher nitrite and HSP70 versus I/R group. Gen.+I/R group showed decreased serum transaminases, lower MPO and ROS versus I/R. Nitrite level was significantly higher in Gen.+I/R versus I/R .Compared to E[2+] I/R or Ral.+I/R, geinstein pretreatment showed significantely lower HSP70. 17-beta estradiol pretreatment produced significant protection during hepatic I/R injury through multiple pathways. The protection was related to HSP70 induction, greater NO release, inhibition of ROS and MPO activity in liver tissue. Both the synthetic estrogen receptor modulator [SERM] raloxifene and the phytoestrogen genistein exerted significant estrogen agonist protective effects on the liver I/R injury. The mechanism of raloxifene protection was similar to estradiol, although protection due to genistein pretreatment did not involve HSP70 induction. Protection by genistein could be attributed to enhanced NO release and inhibition of ROS

Expression of HSP70 in peripheral lymphocytes of the patients with allergic rhinitis / 华中科技大学学报（医学）（英德文版）

The expression levels of heat shock protein 70 (HSP70) from peripheral lymphocytes of the patients with allergic rhinitis (AR) and the clinical implication were investigated. In the morning, 3 ml of fasting venous blood was taken out. The lymphocytes were isolated by using Ficoll-Hypaque and the expression of HSP70 in the lymphocytes was detected by using Western blot. In the AR patients the HSP70 level (41.49 +/- 15.77 integrated optical density, IOD) were significantly higher than that in the control group (23.89 +/- 10.13 IOD, P < 0.05). Western blot demonstrated that HSP70 bands in AR patients were more intensive than those in the control group. It was concluded that the elevated HSP70 level in peripheral lymphocytes of the AR patients might contribute to the development of AR.