RESUMO
Rice starch has been used in various agri-food products due to its hypoallergenic properties. However, rice starch has poor solubility, lower resistant starch content with reduced retrogradation and poor functional properties. Hence, its industrial applications are rather limited. The lack of comprehensive information and a holistic understanding of the interaction between rice starch and endo/exogenous constituents to improve physico-chemical properties is a prerequisite in designing industrial products with enhanced functional attributes. In this comprehensive review, we highlight the potentials of physically mixing of biopolymers in upgrading the functional characteristics of rice starch as a raw material for industrial applications. Specifically, this review tackles rice starch modifications by adding natural/synthetic polymers and plasticizers, leading to functional blends or composites in developing sustainable packaging materials, pharma- and nutraceutical products. Moreover, a brief discussion on rice starch chemical and genetic modifications to alter starch quality for the deployment of rice starch industrial application is also highlighted.
Assuntos
Biopolímeros/química , Oryza/química , Amido/química , Materiais Biocompatíveis/química , Biopolímeros/isolamento & purificação , Biotecnologia , Celulose/química , Fracionamento Químico , Quitosana , Suplementos Nutricionais , Estrutura Molecular , Pectinas/química , Alga Marinha/química , Amido/isolamento & purificaçãoRESUMO
The nanoparticles for the preparation of nanocomposite starch films were synthesized from potato starch using the acid hydrolysis method. The films were prepared by incorporating starch nanoparticles into the film formulation at 0.5, 1, 2, 5, and 10% level of total starch. The control starch film was prepared without the incorporation of starch nanoparticles (SNPs) in film formulation. The starch and SNPs were analyzed for physicochemical and morphological properties. The absorption capacity of SNPs for water and oil was significantly (p < 0.05) lower as compared to native starch. Whereas, the swelling power and solubility of SNPs were significantly (p < 0.05) higher than the swelling power and solubility of starch, respectively. The starch granules were oval and spherical with regular surfaces whereas the SNPs had irregular cracked exteriors spaces. The water vapor transmission rate (WVTR) from nanocomposite starch films was significantly (p < 0.05) lower than the control starch film. The burst strength of films was increased significantly (p < 0.05) with an increased level of SNPs incorporation in film formulation. The incorporation of SNPs increased film thickness and biodegradability. Thus, the present study revealed that the incorporation of SNPs in film formulation resulted in improved film properties.
Assuntos
Nanocompostos , Nanopartículas , Solanum tuberosum/química , Amido/isolamento & purificação , Ácidos/química , Concentração de Íons de Hidrogênio , Hidrólise , Solubilidade , Óleo de Soja/química , Propriedades de Superfície , Resistência à Tração , Água/químicaRESUMO
A new starch was isolated from ramie root, and its physicochemical properties were investigated. Ramie dry root contained 45.9% starch. Starch had truncated, ellipsoidal, and spherical granule shapes with size from 7 to 30 µm and D[4,3] about 14.1 µm. Starch contained 38.9% apparent amylose content and 22.4% true amylose content, exhibited B-type crystallinity, and had 26.6% relative crystallinity, 0.82 ordered degree, and 9.2 nm lamellar thickness. Starch had 71.8 °C gelatinization peak temperature and 15.6 J/g gelatinization enthalpy, and exhibited 31.4 g/g swelling power and 17.1% water solubility at 95 °C. Starch had peak, hot, breakdown, final, and setback viscosities at 3048, 2768, 279, 4165, and 1397 mPa s, respectively, and showed peak time at 4.36 min and pasting temperature at 75.0 °C. The native, gelatinized, and retrograded starches contained 15.1%, 94.0%, and 86.5% rapidly digestible starch and 83.3%, 4.0%, and 10.7% resistant starch, respectively. Compared with potato and rice starches, ramie starch was somewhat similar to potato starch but significantly different from rice starch in starch component, crystalline structure, and functional properties. Therefore, ramie starch exhibited the potential to be used as a thickening agent, resistant-digesting food additive, and alternative to potato starch in food and nonfood industries.
Assuntos
Boehmeria/química , Amido/química , Indústria Alimentícia , Estrutura Molecular , Oryza/química , Raízes de Plantas/química , Solanum tuberosum/química , Amido/isolamento & purificação , Difração de Raios XRESUMO
BACKGROUND: The use of damaged beans for starch isolation comprises an end-use alternative for a product that is not accepted by the consumer. For that reason, isolation and modification of Carioca bean starch should be explored and evaluated as a suitable source for biodegradable material. The present study aimed to evaluate the synergism of physical and chemical modifications on Carioca bean starch with respect to improving the properties of biodegradable films. A heat-moisture treatment (HMT) followed by oxidation by sodium hypochlorite was performed and vice versa. RESULTS: Synergism was noted in the starch properties compared to the single modification. When the oxidation was applied first, a higher amylose and carbonyl content was noted. HMT, isolated and as a second modification, caused a more pronounced effect on viscosity profile than the oxidized starch, with an increase in paste temperature and a decrease in viscosity, breakdown and final viscosity. CONCLUSION: The results obtained in the present study reflect a decrease in water vapor permeability, although a higher tensile strength was noted when oxidation was applied, as a single and as a first modification. © 2020 Society of Chemical Industry.
Assuntos
Fabaceae/química , Extratos Vegetais/química , Amido/química , Oxirredução , Permeabilidade , Extratos Vegetais/isolamento & purificação , Amido/isolamento & purificação , Vapor/análise , Resistência à Tração , ViscosidadeRESUMO
This study describes the effects of hydroxypropylation (HP) on sorghum and corn cold water soluble (CWS) starches prepared via alcoholic alkaline treatment (AAT). Propylene oxide (5% and 12% on starch weight basis) was used to modify both sorghum and corn starches. SEM analysis revealed that HP modification prior to AAT altered the granular morphology of native CWS starches. The characteristic peaks at 2980.28 cm-1 and 2979.87 cm-1 indicated the presence of hydroxypropyl groups and the complete loss of the granular order for HS12-CWS (hydroxypropylated sorghum CWS starch treated with 12% propylene oxide based on dry starch weight) and HC12-CWS (hydroxypropylated corn CWS starch treated with 12% propylene oxide based on dry starch weight) starches. Increase in swelling power and water binding capacity of HP modified CWS starches was observed. However, Percent transmittance was significantly reduced due to fragmented water-soluble granules. HP-modified CWS starch gels exhibited a more rigid gel network. Broader linear viscoelastic range suggesting greater stability and well dispersed behavior of HP-CWS starches. High G' values of HP-CWS starches were due to the ordered and elastic gel network that resisted deformation. Furthermore, all HP-CWS starches exhibited higher shear and thermal resistance compared to unmodified CWS starches.
Assuntos
Celulose/química , Sorghum/química , Zea mays/química , Celulose/análogos & derivados , Celulose/isolamento & purificação , Grão Comestível , Compostos de Epóxi , Reologia , Solubilidade , Amido/química , Amido/isolamento & purificação , Água/químicaRESUMO
Ariá (Goeppertia allouia) is a tuber from the arrowroot's family widely found in the Brazilian Amazon. The tuber has a flavor similar to corn, besides high retrogradation when cooked, differing from other commercial starches. To enhance its added value, the Ariá starch was extracted to evaluate its potential as a food ingredient. The Ariá starch was compared to the commercially available corn and potato starches regarding their physicochemical, thermal, structural, and rheological properties based on the Duncan's test (p-value <0.05). The Ariá starch presented high amylose content (~38% w/w). Furthermore, the X-ray diffraction pattern confirmed its Type-C crystalline structure. The rheological properties showed that the starch gels presented high hardness and retrogradation as other studied starches. Ariá has great potential as a source of starch with low digestibility, increasing the satiety of food products.
Assuntos
Marantaceae/metabolismo , Tubérculos/química , Amido/química , Amilose/química , Brasil , Géis/química , Reologia , Solanum tuberosum/química , Solubilidade , Amido/isolamento & purificação , Difração de Raios X/métodos , Zea mays/químicaRESUMO
Starch based gluten-free bread (formulations containing mixture of corn and potato starch with hydrocolloids) are deficient in nutrients and do not contain health promoting compounds. Therefore they could be supplemented with raw materials rich in such components, especially antioxidants. Among them pseudo-cereals, seeds, fruits and vegetables are often applied to this purpose. Potato pulp produced by processing red fleshed (Magenta Love) and purple fleshed (Violetta) varieties could become a new innovative substrate for gluten-free bread enrichment, because of high levels of endogenous polyphenols, namely flavonoids, flavonols, phenolic acids and especially anthocyanins with high antioxidant potential, as well as dietary fiber. Study material consisted of gluten-free bread enriched in the pulp. Dietary fiber, acrylamide content and antioxidant and antiradical potential of the bread were determined. Sensory evaluation included crumb elasticity, porosity and other characteristics, taste and smell. Among all analyzed gluten-free breads, the sample containing 7.5% share of freeze-dried red potato pulp Magenta Love was characterized by high content of phenolic compounds and dietary fiber, pronounced antioxidant activity, low levels of potentially dangerous acrylamide and good physical and sensory characteristics. Therefore such an addition (7.5% Magenta Love) could be recommended for industrial production of gluten-free bread.
Assuntos
Pão , Farinha/análise , Ingredientes de Alimentos/análise , Solanum tuberosum/química , Verduras/química , Acrilamida/análise , Antioxidantes/análise , Doença Celíaca/dietoterapia , Doença Celíaca/imunologia , Fibras na Dieta/análise , Liofilização/métodos , Glutens/imunologia , Humanos , Valor Nutritivo , Polifenóis/análise , Porosidade , Olfato , Amido/isolamento & purificação , PaladarRESUMO
Common beans (Phaseolus vulgaris L.) are rich in starch with a high content of amylose, which is associated with the production of retrograded and pregelatinized starch through thermal treatments. The purpose of this study was to evaluate the composition, morphology, thermal, functional, and physicochemical properties of carbohydrate extracts (CE) obtained from autoclaved (100 and 121 °C) and extruded (90, 105, and 120 °C) black beans. After evaluation of the functional properties, the CE from autoclaved beans at 100 °C for 30 min and 121 °C for 15 min 2×, and extruded beans at 120 °C and 10 rpm, were selected to continue the remaining analysis. Autoclaving treatments at 100 °C for 30 min and 121 °C for 15 min 2× showed a reduction of resistant starch by 14.4% and 26.6%, respectively, compared to dehulled raw bean CE. Meanwhile, extrusion showed a reduction in resistant starch of 54.2%. Autoclaving and extrusion treatments also decreased the dietary fiber content. Extrusion reduced almost entirely the content of α-galactooligosaccharides, in comparison to dehulled raw bean CE. The results showed differences in color and granule morphology. The onset, peak, and conclusion temperatures, transition temperature range, and enthalpy of autoclaved and extruded bean CE were lower than dehulled raw bean CE. The CE from autoclaved and extruded beans contain retrograded and pregelatinized starch, which could be incorporated in food products as a thickening agent for puddings, sauces, creams, or dairy products. PRACTICAL APPLICATION: Thermally treated black bean carbohydrate extracts are rich in starch, fiber, and protein. Because these extracts are already cooked, they can be added to products that do not require a thermal process such as puddings, sauces, creams, or dairy products, acting as a thickening agent.
Assuntos
Amilose/química , Culinária/métodos , Phaseolus/química , Extratos Vegetais/química , Amido/química , Amilose/isolamento & purificação , Culinária/instrumentação , Fibras na Dieta/análise , Sementes/química , Amido/isolamento & purificação , TemperaturaRESUMO
Starch nanoparticles (SNP) were prepared from different botanical sources (wheat, potato, mung bean, water chestnut and mango kernels) and these were further coupled with zinc oxide (ZnO) to form starch nanocomposites. The nanocomposites were characterized for their particle size, morphological properties, energy dispersive X-ray spectroscopy (EDX) and their biocompatibility was analyzed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, using HeLa cells. From the morphological results, it was observed that ZnO forms super molecules with SNP. Further, EDX studies also confirmed the presence of zinc in coupled molecules. The size distribution of ZnO coupled SNP from different botanical sources revealed that the average diameter of nanocomposites ranged between 506 and 1209 nm. ZnO coupled starch nanocomposites were found to be biocompatible with 77-90% cell viability up to 24 h on HeLa cells. Among all botanical sources studied, ZnO coupled mung bean starch nanocomposite showed the highest cell viability (75% up to 50 h) while ZnO coupled potato starch nanocomposite showed the lowest cell viability (65% up to 50 h).
Assuntos
Nanocompostos/química , Nanopartículas/química , Plantas/química , Amido/química , Óxido de Zinco/química , Materiais Biocompatíveis/química , Sobrevivência Celular/efeitos dos fármacos , Eleocharis/química , Células HeLa , Humanos , Mangifera/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Nanocompostos/ultraestrutura , Nanopartículas/ultraestrutura , Tamanho da Partícula , Solanum tuberosum/química , Espectrometria por Raios X , Amido/isolamento & purificação , Triticum/química , Vigna/químicaRESUMO
Sweet potato is attracting increased research attention because of its high nutritional value (e.g., carotene, anthocyanin, and minerals) and the wide application of its starch in foods and nonfoods. Herein, eight Chinese sweet potato varieties were investigated in terms of the physicochemical properties of starches. The lightness values of the eight sweet potato starches were higher than 90, which was satisfactory for starch purity. The average molecular weight (MW) and amylopectin average chain length (ACL) of sweet potato starches ranged from 6.93 × 107 g/mol to 16.57 × 107 g/mol and from 21.85% to 23.00%, respectively. Su16 starch with low amylose content and a large amount of short chains exhibited low crystallinity and thermal properties. These results suggested that the molecular structure of amylose and amylopectin was the main influencing factor in determining sweet potato starch physicochemical properties. The swelling power and water solubility of the starches ranged within 20.14-30.51 g/g and 5.28%-11.71% at 95 °C, respectively. Regarding pasting properties, all the starch samples presented high peak viscosity (>5500 cP) and peak temperature (>78 °C), indicating that sweet potato starch can be used as a thickener. All eight sweet potatoes varieties showed great application potential in the food industry.
Assuntos
Solanum tuberosum/química , Solanum tuberosum/metabolismo , Amido/química , Amido/isolamento & purificação , Amilose/química , Amilose/metabolismo , Temperatura , ViscosidadeRESUMO
Gelatinization and retrogradation characteristics of starches from tigernut (Cyperus esculentus) tuber before and after various oil extraction processes were studied in this investigation. The results indicated that starches isolated from tigernut tuber after the various oil extraction processes varied significantly in gelatinization and retrogradation properties. The starches isolated from the cakes of tigernut tuber after hot press extraction exhibited higher retrogradation tendency and relatively less shear-thinning than other starch samples. The results of FT-IR, XRD, and NMR analysis indicated that oil extraction had an unfavorable influence on starch retrogradation, which may be due to structural changes caused by oil extraction processes. In particular, oil extraction led to more efficient packing of double helices in the crystalline lamella of the starches during storage. Retrogradation of the starch gels also reduced the water holding capacities of the starches. The starch sample isolated from the cake after cold press extraction exhibited the highest water absorption capacity among the five samples for all storage times. This investigation provides valuable novel information for the industrial utilization of tigernut tuber starches isolated from meals and cakes after oil extraction.
Assuntos
Cyperus/química , Gelatina/química , Óleos de Plantas/isolamento & purificação , Tubérculos/química , Amido/química , Fracionamento Químico/métodos , Cristalização , Tecnologia de Alimentos , Géis/química , Espectroscopia de Ressonância Magnética , Microscopia Eletrônica de Varredura , Pomadas/química , Óleos de Plantas/química , Reologia , Resistência ao Cisalhamento , Espectroscopia de Infravermelho com Transformada de Fourier , Amido/isolamento & purificação , Amido/ultraestrutura , Termogravimetria , Viscosidade , Água/química , Difração de Raios XRESUMO
Global warming may affect the development of rice at different growth stages, thereby decreasing rice yield and deteriorating grain quality. The difference in rice responses to high temperature during primordial differentiation (PD) and pollen filling (PF) stages has been rarely studied. In this paper, two temperature treatments (40 °C and 30 °C) at the two stages (PD and PF) were imposed to four rice groups under the controlled temperature chambers. Compared with rice under normal temperature, high temperature-stressed rice showed accelerated growth rate, smaller caryopsis and decreased yield. Moreover, high temperature affected the starch physicochemical properties, resulting in lower apparent amylose content and higher order degree, gelatinization temperatures, and thereby increased peak, trough and final viscosities in starch. High temperature during PD stage inhibited cell development and starch deposition, thus leading to small starch granule and low retrogradation. However, temperature-stressed rice during PF stage showed increased starch accumulation and larger granule size. Therefore, effects of high temperature during the two stages on caryopsis development and starch properties were partly similar but also notably different. These results enriched and deepened the study of high temperature-stressed rice and served as an important reference for the processing and utilization of rice starch in food industry.
Assuntos
Amilose/química , Inflorescência/metabolismo , Oryza/metabolismo , Pólen/metabolismo , Amido/química , Adaptação Fisiológica , Amilose/isolamento & purificação , Grão Comestível , Temperatura Alta , Humanos , Inflorescência/química , Inflorescência/crescimento & desenvolvimento , Oryza/química , Oryza/crescimento & desenvolvimento , Pólen/química , Pólen/crescimento & desenvolvimento , Amido/isolamento & purificação , Estresse Fisiológico , ViscosidadeRESUMO
Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content. In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes. Starch extracted from juçara seed (JS) was characterized in approximate composition, crystallinity, thermal profile, morphology, and equilibrium moisture data. Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP). JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces. JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve). Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]). JSS-F presented B-type crystallinity and conventional starch-like thermal stability. JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data. As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries. PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits. Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value. This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye. Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.
Assuntos
Euterpe/química , Extratos Vegetais/análise , Amido/análise , Antocianinas/análise , Antocianinas/isolamento & purificação , Antioxidantes/análise , Antioxidantes/isolamento & purificação , Fibras na Dieta/análise , Farinha/análise , Frutas/química , Glicosídeos/análise , Glicosídeos/isolamento & purificação , Valor Nutritivo , Fenóis/análise , Fenóis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Sementes/química , Amido/isolamento & purificação , Resíduos/análiseRESUMO
Structural modification of starch using efficient α-amylases to improve its properties is an established method in the starch industry. In our previous research, the novel maltogenic α-amylase CoMA that catalyzes multi-molecular reactions has been identified. In this study, the impact of CoMA on the structure and retrogradation properties of potato starch was evaluated. CoMA cleaves internal starch chains to change the proportion of amylose and amylopectin in starch. Following treatment, visible pores and microporous on the surface of starch granules were observed from SEM analysis. CoMA modification led to increased insoluble blue complex formation and hydrolysis to shorten the outer chains, which was found to reduce the development rate of starch according to network interactions from the dynamic rheological analysis. Furthermore, maltose accumulation with water competition was also deduced to be involved in the inhibition of retrogradation. Its activities in the cleavage of internal starch granules, shortening of outer chains of starch, and maltose formation make CoMA a powerful agent for the inhibition of starch retrogradation with a very low effective dose of 0.5 mg/kg, which may find potential applications in the starch processing industry.
Assuntos
Proteínas de Bactérias/química , Solanum tuberosum/química , Amido/química , alfa-Amilases/química , Proteínas de Bactérias/isolamento & purificação , Tecnologia de Alimentos/métodos , Humanos , Hidrólise , Maltose/química , Myxococcales/química , Myxococcales/enzimologia , Porosidade , Solubilidade , Amido/isolamento & purificação , Água/química , alfa-Amilases/isolamento & purificaçãoRESUMO
The present study isolated starch from corn and sorghum grains through wet milling procedure. Sorghum starch is considered an alternative to corn starch in future, due to similar functional properties. However, agronomically sorghum is a cheap input cost crop compared to sorghum and can grow in drought hit areas. Lactic acid and citric acid modifications along with heat-moisture treatments were performed on both sorghum and corn grains followed by their comparison in terms of functional, textural, thermal, pasting and digestibility characteristics. For both corn and sorghum starches, the resistant starch increased after chemical modifications. The RS content of acid and acid-heat moisture treated starches were in the range of 77.9-90%, significantly higher than those of native starches (64.6-68.8%). The modifications increased the gelatinization temperature, decreased the peak and cold paste viscosity of starches. Chewiness significantly reduced after lactic and citric acid treatment along with heat-moisture treatments. The crystallinity to amorphous ratio measured through Fourier Transform infrared reduced after all chemical treatments. Percent light transmittance was further reduced after heat-moisture treatments, however the effect on corn starch was more pronounced i.e. it declined from 16.5 to 5.2%. The acid-heat moisture treatments had considerably lowered the glycemic index of starch. The GI reduced from 74 to 49.7 and 60 to 48.5 when treated with acid in the presence of heat and moisture. Thus, these starches could be used in production of low-calorie foods.
Assuntos
Ácido Cítrico/química , Índice Glicêmico , Ácido Láctico/química , Sorghum/química , Amido/química , Zea mays/química , Fenômenos Químicos , Ácido Cítrico/farmacologia , Índice Glicêmico/efeitos dos fármacos , Hidrólise , Ácido Láctico/farmacologia , Tamanho da Partícula , Solubilidade , Análise Espectral , Amido/isolamento & purificação , Amido/ultraestrutura , TermodinâmicaRESUMO
Lycium ruthenicum Murr. is a functional food with abundant anthocyanins. Since anthocyanins can change colors under different pH conditions, pH-sensitive packaging films were developed based on cassava starch and L. ruthenicum anthocyanins (LRA). Effect of LRA content on the physical, structural, antioxidant and pH-sensitive properties of starch-LRA films were evaluated. In addition, starch-LRA films were applied to monitor the freshness of pork. Spectroscopic analysis showed LRA contained six kinds of anthocyanins. The incorporation of LRA significantly enhanced the water vapor and ultraviolet-visible light barrier ability, tensile strength and antioxidant potential of starch film. Moreover, the barrier, antioxidant and pH-sensitive properties of starch-LRA films were closely related with LRA content. However, the thermal stability of starch film was not affected by LRA. Fourier transform infrared and X-ray diffraction analyses revealed intermolecular interactions (hydrogen bonds) formed between starch and LRA in the films. Starch-LRA films are pH-sensitive and could change their colors in different buffer solutions (pHâ¯2-13). When applied to monitor the freshness of pork, starch-LRA films exhibited remarkable color variations with the quality change of pork. Our results suggested starch-LRA films could be used as active and intelligent packaging films in food industry.
Assuntos
Antocianinas/química , Embalagem de Alimentos , Lycium/química , Manihot/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Amido/química , Antocianinas/isolamento & purificação , Materiais Biocompatíveis/química , Materiais Biocompatíveis/isolamento & purificação , Materiais Biocompatíveis/farmacologia , Fenômenos Químicos , Teste de Materiais , Fenômenos Mecânicos , Compostos Fitoquímicos/química , Extratos Vegetais/isolamento & purificação , Análise Espectral , Amido/isolamento & purificaçãoRESUMO
BACKGROUND: The natural variation of starch phosphate content in potatoes has been previously reported. It is known that, in contrast to raw starch, commercially phosphorylated starch is more stable at high temperatures and shear rates and has higher water capacity. The genetic improvement of phosphate content in potato starch by selection or engineering would allow the production of phosphorylated starch in a natural, environmentally friendly way without chemicals. The aim of the current research is to identify genomic SNPs associated with starch phosphorylation by carrying out a genome-wide association study in potatoes. RESULTS: A total of 90 S. tuberosum L. varieties were used for phenotyping and genotyping. The phosphorus content of starch in 90 potato cultivars was measured and then statistically analysed. Principal component analysis (PCA) revealed that the third and eighth principal components appeared to be sensitive to variation in phosphorus content (p = 0.0005 and p = 0.002, respectively). PC3 showed the correlation of starch phosphorus content with allelic variations responsible for higher phosphorylation levels, found in four varieties. Similarly, PC8 indicated that hybrid 785/8-5 carried an allele associated with high phosphorus content, while the Impala and Red Scarlet varieties carried alleles for low phosphorus content. Genotyping was carried out using an Illumina 22 K SNP potato array. A total of 15,214 scorable SNPs (71.7% success rate) was revealed. GWAS mapping plots were obtained using TASSEL based on several statistical models, including general linear models (GLMs), with and without accounting for population structure, as well as MLM. A total of 17 significant SNPs was identified for phosphorus content in potato starch, 14 of which are assigned to 8 genomic regions on chromosomes 1, 4, 5, 7, 8, 10, and 11. Most of the SNPs identified belong to protein coding regions; however, their allelic variation was not associated with changes in protein structure or function. CONCLUSIONS: A total of 8 novel genomic regions possibly associated with starch phosphorylation on potato chromosomes 1, 4, 5, 7, 8, 10, and 11 was revealed. Further validation of the SNPs identified and the analysis of the surrounding genomic regions for candidate genes will allow better understanding of starch phosphorylation biochemistry. The most indicative SNPs may be useful for developing diagnostic markers to accelerate the breeding of potatoes with predetermined levels of starch phosphorylation.
Assuntos
Polimorfismo de Nucleotídeo Único , Solanum tuberosum/genética , Solanum tuberosum/metabolismo , Amido/metabolismo , Cromossomos de Plantas , Estudo de Associação Genômica Ampla , Fosforilação , Tubérculos/química , Tubérculos/metabolismo , Federação Russa , Solanum tuberosum/química , Solanum tuberosum/enzimologia , Amido/isolamento & purificaçãoRESUMO
Nitrogen is an essential nutrient for Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) because this substance affects the yield and quality of Tartary buckwheat. The physicochemical characteristics of starch represent the important parameters of Tartary buckwheat quality. The effects of different levels of nitrogen application at different levels (0, 45, 135 and 225kg/ha in 2015 and 0, 90, 180 and 270kg/ha in 2017) on the physicochemical characteristics of Tartary buckwheat starch were studied. The amylose content, particle size and retrogradation of Tartary buckwheat starch with nitrogen were lower than those of without nitrogen. The structure complexity, pasting temperature, gelatinization enthalpy, relative crystallinity, light transmittance and solubility of the former were higher than those of the latter. Nitrogen application did not change the 'A'-type crystalline pattern of Tartary buckwheat starch. This study indicated that nitrogen level and years and the interactions among nitrogen fertilizer levels and years significantly affected the physicochemical properties of Tartary buckwheat starch. These integrated results also provided information about the management of fertilization conditions to obtain starches with special properties for applications in food or nonfood industries.
Assuntos
Fenômenos Químicos , Fagopyrum/química , Fagopyrum/metabolismo , Nitrogênio/metabolismo , Amido/química , Amido/metabolismo , Amilose/química , Tamanho da Partícula , Solubilidade , Análise Espectral , Amido/isolamento & purificação , Amido/ultraestrutura , TemperaturaRESUMO
Expansion characteristics of cornstarch-based extrudates incorporating fiber-rich food processing byproducts was explored. Waxy and regular cornstarch were used as the base materials with apple pomace and sugarcane bagasse incorporated at two addition levels (0%, 15%, and 30% w/w). Extrusions were conducted at three different screw speeds (150, 200, and 250 rpm) with other parameters optimized and kept constant. Apple pomace inclusion resulted in higher initial expansion index (4.23 to 5.60) and higher stable expansion index (2.76 to 4.43), but also showed higher shrinkage (8.50% to 34.72%) than sugarcane bagasse extrudates at the same inclusion levels. Inclusion of apple pomace showed potential of producing extrudates with significantly higher expansion than cornstarch control, with relatively lower energy inputs. Extrusion methods used here have the potential to preserve the textural quality and nutritional value of the fiber-enriched extrudates, providing the base for healthier snack food items. PRACTICAL APPLICATION: Findings from this study can be extended to the other fiber-rich food processing byproducts, such as other fruit and vegetable pomace, cereal brans, and pulse hulls among other materials. This data will help the development of fiber-enriched extruded snacks that would have favorable consumer traits.